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Maxime Madder - One of the best experts on this subject based on the ideXlab platform.
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invasive cattle ticks in east africa morphological and molecular confirmation of the presence of Rhipicephalus microplus in south eastern uganda
Parasites & Vectors, 2020Co-Authors: Dennis Muhanguzi, Joseph K Byaruhanga, Robert Tweyongyere, Wilson Amanyire, Christian Ndekezi, Sylvester Ochwo, Joseph Nkamwesiga, Frank Norbert Mwiine, Josephus J Fourie, Maxime MadderAbstract:Rhipicephalus microplus, an invasive tick species of Asian origin and the main vector of Babesia species, is considered one of the most widespread ectoparasites of livestock. The tick has spread from its native habitats on translocated livestock to large parts of the tropical world, where it has replaced some of the local populations of Rhipicephalus decoloratus ticks. Although the tick was reported in Uganda 70 years ago, it has not been found in any subsequent surveys. This study was carried out to update the national tick species distribution on livestock in Uganda as a basis for tick and tick-borne disease control, with particular reference to R. microplus. The study was carried out in Kadungulu, Serere district, south-eastern Uganda, which is dominated by small scale livestock producers. All the ticks collected from 240 cattle from six villages were identified microscopically. Five R. microplus specimens were further processed for phylogenetic analysis and species confirmation. The predominant tick species found on cattle was Rhipicephalus appendiculatus (86.9 %; n = 16,509). Other species found were Amblyomma variegatum (7.2 %; n = 1377), Rhipicephalus evertsi (2.3 %; n = 434) and R. microplus (3.6 %; n = 687). Phylogenetic analysis of the 12S rRNA, 16S rRNA and ITS2 gene sequences of R. microplus confirmed the morphological identification. It is concluded that R. microplus has replaced R. decoloratus in the sampled villages in Kadungulu sub-county, since the latter was not any longer found in this area. There is currently no livestock movement policy in force in Uganda, which could possibly limit the further spread of R. microplus ticks. Future surveys, but also retrospective surveys of museum specimens, will reveal the extent of distribution of R. microplus in Uganda and also for how long this tick has been present on livestock without being noticed.
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A comparative test of ixodid tick identification by a network of European researchers
Ticks and Tick-borne Diseases, 2017Co-Authors: Agustin Estrada-pena, Manoj Fonville, Gianluca D'amico, Ana Maria Palomar, Marlène Dupraz, Dieter J.a. Heylen, Miguel A. Habela, Sandor Hornok, Laetitia Lempereur, Maxime MadderAbstract:This study reports the results of a comparative test of identification of ticks occurring in Western Europe and Northern Africa. A total of 14 laboratories were voluntarily enrolled in the test. Each participant received between 22 and 25 specimens of adult and nymphal ticks of 11 species: Dermacentor marginatus, D. reticulatus, Haemaphysalis punctata, Hyalomma lusitanicum, Hy. marginatum, Ixodes ricinus, I. hexagonus, Rhipicephalus annulatus, R. bursa, R. rossicus, and/or R. sanguineus s.l. Ticks were morphologically identified by three of the co-authors and the identification confirmed by a fourth co-author who used molecular methods based on several genes. Then ticks were randomly selected and blindly distributed among participants, together with a questionnaire. Only specimens collected while questing and, if possible, in the same survey, were circulated. Because of the random nature of the test, a participant could receive several specimens of the same species. Species in the different genera had variable misidentification rates (MR) of 7% (Derrnacentor), 14% (Ixodes), 19% (Haemapbysalis), 36% (Hyalomma), and 54% (Rhipicephalus). Within genera, the MR was also variable ranging from 5.4% for I. ricinus or 7.4% for D. marginatus or D. reticulatus to 100% for R. rossicus. The test provided a total misidentification rate of 29.6% of the species of ticks. There are no significant differences in MR according to the sex of the tick. Participants were requested to perform a second round of identifications on the same set of ticks, using only purposely prepared keys (without illustrations), circulated to the enrolled participants, including 2 species of the genus Dermacentor, 8 of Haemaphysalis, 10 of Hyalomma, 23 of Ixodes, and 6 of Rhipicephalus. The average MR in the second round was 28%: 0% (Dermacentor), 33% (Haemaphysalis), 30% (Hyalomma) 18% (Ixodes), and 50% (Rhipicephalus). Species which are not reported in the countries of a participating laboratory had always highest MR, i.e. purely Mediterranean species had highest MR by laboratories in Central and Northern Europe. Participants expressed their concerns about a correct identification for almost 50% of the ticks of the genera Hyalomma and Rhipicephalus. The results revealed less than total confidence in identifying the most prominent species of ticks in the Western Palearctic, and underpin the need for reference libraries for specialists involved in this task. Results also showed that a combination of certain genes may adequately identify the target species of ticks.
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Seasonal abundance of ticks (Acari/ Ixodidae) infesting cattle in four irrigated regions in Morocco
Institut Agronomique et Vétérinaire Hassan II, 2016Co-Authors: Tarik Rahali, Maxime Madder, Abdelkbir Ghalem, Abderrahim Sadak, Sanaa Aithamou, Aouatif Saadi, Bertrand Losson, Hamid SahibiAbstract:A study has been conducted between April 2007 and March 2008 to identify tick species infesting cattle in four irrigated regions in Morocco and to determine their abundance and phenology. The influence of climate, type of farm, age, sex and origin of the animals on the tick infestation was analyzed. 5,777 ticks have been collected and ten species identified, Hyalomma scupense 1,544 (26.7%), Hyalomma marginatum 1,489 (25.8%), Hyalomma lusitanicum 199 (3.4%), Hyalomma excavatum 554 (6.1%), Hyalomma dromedarii 59 (1.0%), Hyalomma impeltatum 26 (0.5%), Rhipicephalus sanguineus 266 (4.6%), Rhipicephalus turanicus 232 (4.0%), Rhipicephalus bursa 391 (10.2%) and Rhipicephalus (Boophilus) annulatus 1,017 (17.6%). The activity of most of the species was limited to spring and summer. According to the study, age and type of farm have a significant influence on the degree of infestation of cattle by ticks, whereas the origin and sex of the animals and climate had less influence
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geographic distribution of the invasive cattle tick Rhipicephalus microplus a country wide survey in benin
Experimental and Applied Acarology, 2012Co-Authors: E De Clercq, Safiou Adehan, R Lokossou, Sophie O Vanwambeke, Marvelous Sungirai, Maxime MadderAbstract:The cattle tick Rhipicephalus microplus is currently invading the West African region, and little information is available on the spread of this exotic tick in this region. We set out a country-wide field survey to determine its current distribution in Benin. Ticks were collected on cattle from 106 farms selected by random sampling covering all regions of the country. Rhipicephalus annulatus was found on 70 % of all farms, R. decoloratus on 42 %, R. geigyi on 58 %, and R. microplus on 49 %. There is a clear geographic separation between the indigenous Rhipicephalus species and R. microplus. Rhipicephalus annulatus occurs mainly in the northern departments, but it was also observed in lower numbers in locations in the south. The presence of R. decoloratus is limited to the northern region, and in most locations, this tick makes up a small proportion of the collected ticks. The tick R. geigyi tends to be dominant, but occurs only in the four northern departments. The observations concerning R. microplus are entirely different, this species occurs in the southern and central region. The results of this survey confirm the invasive character and displacement properties of R. microplus, since in less than a decade it has colonized more than half of the country and has displaced indigenous ticks of the same genus in many of the sampled locations.
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new foci of Rhipicephalus microplus in west africa
Experimental and Applied Acarology, 2012Co-Authors: Maxime Madder, Safiou Adehan, R Adehan, Redgi De Deken, R LokossouAbstract:The invasive character of Rhipicephalus microplus was observed in Benin, the second West-African country from which this ticks species has been collected after the initial confirmed record in Ivory Coast in 2007. A cross-sectional study was carried out in the Department of Mono to examine the presence of the tick R. microplus. The survey covered 9 herds (villages) in an agro-ecological zone inhabited by agro-pastoralists, including the State Farm of Kpinnou that imported Girolando cattle from Brazil. Almost 800 ticks were sampled from 36 cattle, on average four cattle per village. The morphological identification revealed ticks of two different genera: Rhipicephalus and Amblyomma. Rhipicephalus microplus was the only representative of the species previously known as Boophilus or blue ticks. Its taxonomic identity was confirmed molecularly by PCR–RFLP. A comparison was made with the situation of R. microplus in Brazil.
Domenico Otranto - One of the best experts on this subject based on the ideXlab platform.
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biological compatibility between two temperate lineages of brown dog ticks Rhipicephalus sanguineus sensu lato
Parasites & Vectors, 2018Co-Authors: Maria Stefania Latrofa, Filipe Dantastorres, Rafael Antonio Nascimento Ramos, Riccardo Paolo Lia, Gioia Capelli, Antonio Parisi, Daniele Porretta, Sandra Urbanelli, Domenico OtrantoAbstract:Background The brown dog tick Rhipicephalus sanguineus (sensu stricto) is reputed to be the most widespread tick of domestic dogs worldwide and has also been implicated in the transmission of many pathogens to dogs and humans. For more than two centuries, Rh. sanguineus (s.s.) was regarded as a single taxon, even considering its poor original description and the inexistence of a type specimen. However, genetic and crossbreeding experiments have indicated the existence of at least two distinct taxa within this name: the so-called “temperate” and “tropical” lineages of Rh. sanguineus (sensu lato). Recent genetic studies have also demonstrated the existence of additional lineages of Rh. sanguineus (s.l.) in Europe and Asia. Herein, we assessed the biological compatibility between two lineages of Rh. sanguineus (s.l.) found in southern Europe, namely Rhipicephalus sp. I (from Italy) and Rhipicephalus sp. II (from Portugal).
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detection of anaplasma platys in dogs and Rhipicephalus sanguineus group ticks by a quantitative real time pcr
Veterinary Parasitology, 2014Co-Authors: Rafael Antonio Nascimento Ramos, Maria Stefania Latrofa, Alessio Giannelli, Filipe Dantastorres, Vita Lacasella, Bronwyn E Campbell, Domenico OtrantoAbstract:Anaplasma platys is an obligate intracellular, tick-borne pathogen of dogs, which causes canine infectious cyclic thrombocytopenia (CICT). The vector role of Rhipicephalus sanguineus group ticks has been only suggested, but definitive evidence is lacking. This study aimed to detect and quantify A. platys DNA in infected dogs and in their respective ticks through a quantitative real-time PCR assay. From March to May 2009, blood and tick samples from dogs residing in a CICT-endemic area were collected and molecularly analysed. Differences (p 0.05). The mean bacterial load detected in positive dogs was lower than that in their respective ticks (p>0.05). This study provides circumstantial evidence of the putative role of Rhipicephalus sp. I as a vector of this pathogen.
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Molecular detection of tick-borne pathogens in Rhipicephalus sanguineus group ticks.
Ticks and tick-borne diseases, 2014Co-Authors: Maria Stefania Latrofa, Filipe Dantas-torres, Alessio Giannelli, Domenico OtrantoAbstract:a b s t r a c t The Rhipicephalus sanguineus group includes some of the most widespread dog ticks, whose identifica- tion currently represents a difficult task due to the morphological similarities of these ixodid species. Recently, following the morphological and molecular characterization of tick specimens collected from dogs in all continents, Rhipicephalus sanguineus sensu lato (s.l.), Rhipicephalus turanicus and three different operational taxonomic units (namely Rhipicephalus sp. I-III) have been identified. Therefore, a compre- hensive molecular study has been herein carried out to detect selected canine vector-borne pathogens (i.e., Anaplasma platys, Cercopithifilaria spp., Ehrlichia canis and Hepatozoon canis) in ticks belonging to the R. sanguineus group. A total of 204 tick specimens from dogs were examined and identified as R. sanguineus s.l. (n = 81), R. turanicus (n = 17), Rhipicephalus sp. I (n = 66), Rhipicephalus sp. II (n = 37), and Rhipicephalus sp. III (n = 3). PCR assays were performed to detect mitochondrial and ribosomal target genes of Cercop- ithifilaria spp., A. platys, E. canis and H. canis. Out of 204 specimens examined, 2.5%, 7.4% and 21.6% scored positive to A. platys, H. canis and Cercopithifilaria spp., respectively. In addition, co-infections with two pathogens (i.e., Cercopithifilaria bainae and A. platys or H. canis) were detected in four tick specimens. This study suggests a relationship between ticks belonging to the R. sanguineus group and the geographical distribution of A. platys, H. canis and Cercopithifilaria spp. However, the role of each representative tick species within the R. sanguineus group in the transmission of these canine pathogens needs to be further investigated.
Filipe Dantastorres - One of the best experts on this subject based on the ideXlab platform.
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biological compatibility between two temperate lineages of brown dog ticks Rhipicephalus sanguineus sensu lato
Parasites & Vectors, 2018Co-Authors: Maria Stefania Latrofa, Filipe Dantastorres, Rafael Antonio Nascimento Ramos, Riccardo Paolo Lia, Gioia Capelli, Antonio Parisi, Daniele Porretta, Sandra Urbanelli, Domenico OtrantoAbstract:Background The brown dog tick Rhipicephalus sanguineus (sensu stricto) is reputed to be the most widespread tick of domestic dogs worldwide and has also been implicated in the transmission of many pathogens to dogs and humans. For more than two centuries, Rh. sanguineus (s.s.) was regarded as a single taxon, even considering its poor original description and the inexistence of a type specimen. However, genetic and crossbreeding experiments have indicated the existence of at least two distinct taxa within this name: the so-called “temperate” and “tropical” lineages of Rh. sanguineus (sensu lato). Recent genetic studies have also demonstrated the existence of additional lineages of Rh. sanguineus (s.l.) in Europe and Asia. Herein, we assessed the biological compatibility between two lineages of Rh. sanguineus (s.l.) found in southern Europe, namely Rhipicephalus sp. I (from Italy) and Rhipicephalus sp. II (from Portugal).
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detection of anaplasma platys in dogs and Rhipicephalus sanguineus group ticks by a quantitative real time pcr
Veterinary Parasitology, 2014Co-Authors: Rafael Antonio Nascimento Ramos, Maria Stefania Latrofa, Alessio Giannelli, Filipe Dantastorres, Vita Lacasella, Bronwyn E Campbell, Domenico OtrantoAbstract:Anaplasma platys is an obligate intracellular, tick-borne pathogen of dogs, which causes canine infectious cyclic thrombocytopenia (CICT). The vector role of Rhipicephalus sanguineus group ticks has been only suggested, but definitive evidence is lacking. This study aimed to detect and quantify A. platys DNA in infected dogs and in their respective ticks through a quantitative real-time PCR assay. From March to May 2009, blood and tick samples from dogs residing in a CICT-endemic area were collected and molecularly analysed. Differences (p 0.05). The mean bacterial load detected in positive dogs was lower than that in their respective ticks (p>0.05). This study provides circumstantial evidence of the putative role of Rhipicephalus sp. I as a vector of this pathogen.
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Rhipicephalus sanguineus acari ixodidae the brown dog tick parasitizing humans in brazil
Revista Da Sociedade Brasileira De Medicina Tropical, 2006Co-Authors: Filipe Dantastorres, Luciana Aguiar Figueredo, Sinval Pinto BrandaofilhoAbstract:ABSTRACT The objective of this paper is to describe four cases of human parasitism by Rhipicephalus sanguineus (Latrielle) in Brazil.During an investigation regarding the species of ectoparasites of domestic dogs from the metropolitan region of Recife,Pernambuco state, four dog owners were found to be parasitized by ticks. The ticks were collected from these individuals andtheir dogs. All the ticks were identified as Rhipicephalus sanguineus. These are, to our knowledge, the first four cases ofhuman parasitism by this tick species in Brazil. The possible implications of this finding are discussed here. Key-words : Brown dog tick. Human parasitism. Tick-borne diseases. Epidemiology. RESUMO O objetivo deste artigo e descrever quatro casos de parasitismo humano por Rhipicephalus sanguineus (Latrielle) no Brasil.Durante uma investigacao sobre as especies de ectoparasitas de caes domesticos provenientes da Regiao Metropolitana deRecife, Pernambuco, quatro proprietarios de caes foram encontrados parasitados por carrapatos. Foram coletados carrapatosdos individuos e de seus caes. Todos os carrapatos foram identificados como Rhipicephalus sanguineus, sendo, portanto,descritos os primeiros quatro casos de parasitismo humano por esta especie de carrapato, no Brasil. Neste trabalho, saodiscutidas as possiveis implicacoes epidemiologicas deste achado.
Maria Stefania Latrofa - One of the best experts on this subject based on the ideXlab platform.
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biological compatibility between two temperate lineages of brown dog ticks Rhipicephalus sanguineus sensu lato
Parasites & Vectors, 2018Co-Authors: Maria Stefania Latrofa, Filipe Dantastorres, Rafael Antonio Nascimento Ramos, Riccardo Paolo Lia, Gioia Capelli, Antonio Parisi, Daniele Porretta, Sandra Urbanelli, Domenico OtrantoAbstract:Background The brown dog tick Rhipicephalus sanguineus (sensu stricto) is reputed to be the most widespread tick of domestic dogs worldwide and has also been implicated in the transmission of many pathogens to dogs and humans. For more than two centuries, Rh. sanguineus (s.s.) was regarded as a single taxon, even considering its poor original description and the inexistence of a type specimen. However, genetic and crossbreeding experiments have indicated the existence of at least two distinct taxa within this name: the so-called “temperate” and “tropical” lineages of Rh. sanguineus (sensu lato). Recent genetic studies have also demonstrated the existence of additional lineages of Rh. sanguineus (s.l.) in Europe and Asia. Herein, we assessed the biological compatibility between two lineages of Rh. sanguineus (s.l.) found in southern Europe, namely Rhipicephalus sp. I (from Italy) and Rhipicephalus sp. II (from Portugal).
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detection of anaplasma platys in dogs and Rhipicephalus sanguineus group ticks by a quantitative real time pcr
Veterinary Parasitology, 2014Co-Authors: Rafael Antonio Nascimento Ramos, Maria Stefania Latrofa, Alessio Giannelli, Filipe Dantastorres, Vita Lacasella, Bronwyn E Campbell, Domenico OtrantoAbstract:Anaplasma platys is an obligate intracellular, tick-borne pathogen of dogs, which causes canine infectious cyclic thrombocytopenia (CICT). The vector role of Rhipicephalus sanguineus group ticks has been only suggested, but definitive evidence is lacking. This study aimed to detect and quantify A. platys DNA in infected dogs and in their respective ticks through a quantitative real-time PCR assay. From March to May 2009, blood and tick samples from dogs residing in a CICT-endemic area were collected and molecularly analysed. Differences (p 0.05). The mean bacterial load detected in positive dogs was lower than that in their respective ticks (p>0.05). This study provides circumstantial evidence of the putative role of Rhipicephalus sp. I as a vector of this pathogen.
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Molecular detection of tick-borne pathogens in Rhipicephalus sanguineus group ticks.
Ticks and tick-borne diseases, 2014Co-Authors: Maria Stefania Latrofa, Filipe Dantas-torres, Alessio Giannelli, Domenico OtrantoAbstract:a b s t r a c t The Rhipicephalus sanguineus group includes some of the most widespread dog ticks, whose identifica- tion currently represents a difficult task due to the morphological similarities of these ixodid species. Recently, following the morphological and molecular characterization of tick specimens collected from dogs in all continents, Rhipicephalus sanguineus sensu lato (s.l.), Rhipicephalus turanicus and three different operational taxonomic units (namely Rhipicephalus sp. I-III) have been identified. Therefore, a compre- hensive molecular study has been herein carried out to detect selected canine vector-borne pathogens (i.e., Anaplasma platys, Cercopithifilaria spp., Ehrlichia canis and Hepatozoon canis) in ticks belonging to the R. sanguineus group. A total of 204 tick specimens from dogs were examined and identified as R. sanguineus s.l. (n = 81), R. turanicus (n = 17), Rhipicephalus sp. I (n = 66), Rhipicephalus sp. II (n = 37), and Rhipicephalus sp. III (n = 3). PCR assays were performed to detect mitochondrial and ribosomal target genes of Cercop- ithifilaria spp., A. platys, E. canis and H. canis. Out of 204 specimens examined, 2.5%, 7.4% and 21.6% scored positive to A. platys, H. canis and Cercopithifilaria spp., respectively. In addition, co-infections with two pathogens (i.e., Cercopithifilaria bainae and A. platys or H. canis) were detected in four tick specimens. This study suggests a relationship between ticks belonging to the R. sanguineus group and the geographical distribution of A. platys, H. canis and Cercopithifilaria spp. However, the role of each representative tick species within the R. sanguineus group in the transmission of these canine pathogens needs to be further investigated.
Atiya-nasagi Yafit - One of the best experts on this subject based on the ideXlab platform.
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\u3cem\u3eRickettsia africae\u3c/em\u3e and \u3cem\u3eCandidatus\u3c/em\u3e Rickettsia barbariae in Ticks in Israel
'American Society of Tropical Medicine and Hygiene', 2014Co-Authors: Waner Trevor, Keysary Avi, Eremeeva, Marina E., Din, Adi Beth, Mumcuoglu, Kosta Y., King Roni, Atiya-nasagi YafitAbstract:DNA of several spotted fever group rickettsiae was found in ticks in Israel. The findings include evidence for the existence of Rickettsia africae and CandidatusRickettsia barbariae in ticks in Israel. The DNA of R. africae was detected in aHyalomma detritum tick from a wild boar and DNA of C. Rickettsia barbariae was detected in Rhipicephalus turanicus and Rhipicephalus sanguineuscollected from vegetation. The DNA of Rickettsia massiliae was found in Rh. sanguineus and Haemaphysalis erinacei, whereas DNA of Rickettsia sibirica mongolitimonae was detected in a Rhipicephalus (Boophilus) annulatus. Clinicians should be aware that diseases caused by a variety of rickettsiae previously thought to be present only in other countries outside of the Middle East may infect residents of Israel who have not necessarily traveled overseas. Furthermore, this study reveals again that the epidemiology of the spotted fever group rickettsiae may not only involve Rickettsia conorii but may include other rickettsiae
