The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Ylva Hellsten - One of the best experts on this subject based on the ideXlab platform.
-
the impact of lower limb immobilization and rehabilitation on Angiogenic Proteins and capillarization in skeletal muscle
Medicine and Science in Sports and Exercise, 2021Co-Authors: Lasse Gliemann, Nicolai Rytter, Tue Smith Jorgensen, Peter Piil, Howard H Carter, Michael Nyberg, Marcello Grassi, Martin Daumer, Ylva HellstenAbstract:Purpose Skeletal muscle vascularization is important for tissue regeneration after injury and immobilization. We examined whether complete immobilization influences capillarization and oxygen delivery to the muscle and assessed the efficacy of rehabilitation by aerobic exercise training. Methods Young healthy males had one leg immobilized for 14 days and subsequently completed four weeks of intense aerobic exercise training. Biopsies were obtained from m.vastus lateralis and a-v blood sampling for assessment of oxygen extraction and leg blood flow during exercise was done before and after immobilization and training. Muscle capillarization, muscle and platelet content of vascular endothelial growth factor (VEGF) and muscle thrombospondin-1 were determined. Results Immobilization did not have a significant impact on capillary per fiber ratio or capillary density. The content of VEGF protein in muscle samples was reduced by 36% (P=0.024) and VEGF to thrombospondin-1 ratio was 94 % lower (P=0.046). The subsequent four-week training period increased the muscle VEGF content and normalized the muscle VEGF to thrombospondin-1 ratio but did not influence capillarization. Platelet VEGF content followed the trend of muscle VEGF. At the functional level, oxygen extraction, blood flow and oxygen delivery at rest and during submaximal exercise were not affected by immobilization or training. Conclusion The results demonstrate that just two weeks of leg immobilization leads to a strongly reduced Angiogenic potential as evidenced by reduced muscle and platelet VEGF content and a reduced muscle VEGF to thrombospondin-1 ratio. Moreover, a subsequent period of intensive aerobic exercise training fails to increase capillarization in the previously immobilized leg, possibly due to the angiostatic condition caused by immobilization.
-
early time course of change in Angiogenic Proteins in human skeletal muscle and vascular cells with endurance training
Scandinavian Journal of Medicine & Science in Sports, 2020Co-Authors: Birgitte Hoier, Karina Olsen, Dorte Jessing Agerby Hanskov, Maria Monster Jorgensen, Liselotte Rothmann Norup, Ylva HellstenAbstract:Angiogenic, mitochondrial, and related transcriptional Proteins were assessed in human skeletal muscle and isolated vascular cells during the early phase of endurance training. Thigh muscle biopsies were obtained in healthy young subjects, after one acute bout (n = 9) and after 3, 5, 7, and 14 days (n = 9) of cycle ergometer training. Whole muscle homogenates were analyzed for Angiogenic, mitochondrial, and regulatory mRNA and protein levels. Angiogenic Proteins were determined in muscle-derived endothelial cells and pericytes sorted by fluorescence-activated cell sorting. Acute exercise induced an increase in whole muscle mRNA of peroxisome proliferator-activated receptor gamma coactivator 1α (4.5-fold; P = .002) and vascular endothelial growth factor (VEGF) (2.4-fold; P = .001) at 2 hours post. After 14 days of training, there was an increase in CD31 protein (63%; P = .010) in whole muscle indicating capillary growth. There was also an increase in muscle VEGF receptor 2 (VEGFR2) (1.5-fold; P = .013), in OXPHOS Proteins (complex I, II, IV, V; 1.4- to 1.9-fold; P < .05) after 14 days of training and an increase in estrogen-related receptorα protein (1.5-fold; P = .039) at 14 days compared to 5 days of training. Both endothelial cells and pericytes expressed VEGF and other Angiogenic factors at the protein level but with a distinctively lower expression of VEGFR2 and thrombospondin-1 (TSP-1) in pericytes. The findings illustrate that initiation of capillary and mitochondrial adaptations occurs within 14 days of training and suggest that sustained changes in Angiogenic Proteins including VEGF and TSP-1 are moderate in whole muscle and vascular cells.
Hans-eberhard Schaller - One of the best experts on this subject based on the ideXlab platform.
-
Fetuin-A influences vascular cell growth and production of proinflammatory and Angiogenic Proteins by human perivascular fat cells
Diabetologia, 2014Co-Authors: Dorothea Siegel-axel, Susanne Ullrich, Norbert Stefan, Kilian Rittig, F Gerst, C Klingler, Ulrike Schmidt, Birgit Schreiner, Elko Randrianarisoa, Hans-eberhard SchallerAbstract:Aims/hypothesis Fetuin-A (alpha2-Heremans-Schmid glycoprotein), a liver-derived circulating glycoprotein, contributes to lipid disorders, diabetes and cardiovascular diseases. In a previous study we found that perivascular fat cells (PVFCs) have a higher Angiogenic potential than other fat cell types. The aim was to examine whether fetuin-A influences PVFC and vascular cell growth and the expression and secretion of proinflammatory and Angiogenic Proteins, and whether TLR4-independent pathways are involved.
-
fetuin a influences vascular cell growth and production of proinflammatory and Angiogenic Proteins by human perivascular fat cells
Diabetologia, 2014Co-Authors: Dorothea Siegelaxel, Susanne Ullrich, Norbert Stefan, Kilian Rittig, F Gerst, C Klingler, Ulrike Schmidt, Birgit Schreiner, Elko Randrianarisoa, Hans-eberhard SchallerAbstract:Fetuin-A (alpha2-Heremans-Schmid glycoprotein), a liver-derived circulating glycoprotein, contributes to lipid disorders, diabetes and cardiovascular diseases. In a previous study we found that perivascular fat cells (PVFCs) have a higher Angiogenic potential than other fat cell types. The aim was to examine whether fetuin-A influences PVFC and vascular cell growth and the expression and secretion of proinflammatory and Angiogenic Proteins, and whether TLR4-independent pathways are involved. Mono- and co-cultures of human PVFCs and endothelial cells were treated with fetuin-A and/or palmitate for 6–72 h. Proteins were quantified by ELISA and Luminex, mRNA expression by real-time PCR, and cell growth by BrDU-ELISA. Some PVFCs were preincubated with a nuclear factor κB NFκBp65 inhibitor, or the toll-like receptor 4 (TLR4) inhibitor CLI-095, or phosphoinositide 3-kinase (PI3K)/Akt inhibitors and/or stimulated with insulin. Intracellular forkhead box protein O1 (FoxO1), NFκBp65 and inhibitor of κB kinase β (IKKβ) localisation was visualised by immunostaining. PVFCs expressed and secreted IL-6, IL-8, plasminogen activator inhibitor 1 (PAI-1), basic fibroblast growth factor (bFGF), platelet-derived growth factor (PDGF)-BB, monocyte chemotactic protein-1 (MCP-1), vascular endothelial growth factor (VEGF), placental growth factor (PLGF) and hepatocyte growth factor (HGF). Fetuin-A upregulated IL-6 and IL-8, and this was potentiated by palmitate and blocked by CLI-095. Immunostaining and electrophoretic mobility shift assay (EMSA) showed partial NFκBp65 activation. MCP-1 was upregulated and blocked by CLI-095, but not by palmitate. However, HGF was downregulated, which was slightly potentiated by palmitate. This effect persisted after TLR4 pathway blockade. Stimulation of insulin–PI3K–Akt signalling by insulin resulted in nuclear FoxO1 extrusion and HGF upregulation. Fetuin-A counteracted these insulin effects. Fetuin-A and/or palmitate influence the expression of proinflammatory and Angiogenic Proteins only partially via TLR4 signalling. HGF downregulation seems to be mediated by interference with the insulin-dependent receptor tyrosine kinase pathway. Fetuin-A may also influence Angiogenic and proinflammatory Proteins involved in atherosclerosis.
Tzu-hao Wang - One of the best experts on this subject based on the ideXlab platform.
-
Early anti-Angiogenic Proteins expression in amniotic fluid of twin fetuses.
Twin Research and Human Genetics, 2011Co-Authors: Chao-nin Wang, Jannie Ying-syuan Chen, An-shine Chao, Po-jen Cheng, Shuenn-dyh Chang, Tzu-hao WangAbstract:Multiple pregnancies are thought to be associated with a high incidence of perinatal complications such as preterm labor, preeclampsia and low birth weight. But the true mechanisms of these obstetric complications are still uncertain. The components of amniotic fluid reflect the pathophysiology features of the fetus. Amniotic fluid soluble fms-like tyrosine kinase 1(sFLT1), soluble endoglin (sENG), and adiponectin reflect the oxidative stress and pro-inflammatory status and are associated with preeclampsia and fetal growth restriction. We prospectively collected amniotic fluids during amniocentesis from singleton and twin pregnancies. Samples were analyzed for levels of sFLT1, sENG, and adiponectin by enzyme-linked immunosorbent assay. The levels of sENG and sFLT1 were significantly increased in twin pregnancies. Adiponectin was not significantly different between the two groups. These findings would suggest that twin fetuses suffer from more oxidative stress and pro-inflammatory status from the early trimesters.
-
change in amniotic fluid levels of multiple anti Angiogenic Proteins before development of preeclampsia and intrauterine growth restriction
The Journal of Clinical Endocrinology and Metabolism, 2010Co-Authors: Chao-nin Wang, An-shine Chao, Po-jen Cheng, Shuenn-dyh Chang, Tzu-hao Wang, Hsiuhuei Peng, Yunshien Lee, Yaolung ChangAbstract:Context: The cause of preeclampsia remains unknown. Excessive antiAngiogenic Proteins have been proposed to play a pathogenic role in preeclampsia. Objective: Our objective was to determine the differences in soluble endoglin (sEndoglin), soluble fms-like tyrosine kinase receptor-1 (sFLT1), leptin, adiponectin, and endothelin 1 concentrations between normal and preeclampsia amniotic fluid (AF). Such results may help us understand the pathophysiology of preeclampsia. Methods: We performed a nested case-control study. Seventy-one women with preeclampsia were matched to 71 normotensive controls. The preeclamptic women were broken into two subgroups according to the association with fetal intrauterine growth restriction (IUGR). AF concentrations of sEndoglin, sFLT1, leptin, adiponectin, and endothelin 1 were measured by ELISA. Receiver-operating characteristics curve analysis was used to compare the discriminative values of these potential biomarkers. Functional network analysis was performed using MetaCore t...
Eric Jauniaux - One of the best experts on this subject based on the ideXlab platform.
-
in vitro study of the effect of anti hypertensive drugs on placental hormones and Angiogenic Proteins synthesis in pre eclampsia
PLOS ONE, 2014Co-Authors: S Gangooly, Shanthi Muttukrishna, Eric JauniauxAbstract:Antihypertensive drugs lower the maternal blood pressure in pre-eclampsia (PE) by direct or central vasodilatory mechanisms but little is known about the direct effects of these drugs on placental functions.
-
in vitro study of the effect of anti hypertensive drugs on placental hormones and Angiogenic Proteins synthesis in pre eclampsia
PLOS ONE, 2014Co-Authors: S Gangooly, Shanthi Muttukrishna, Eric JauniauxAbstract:Antihypertensive drugs lower the maternal blood pressure in pre-eclampsia (PE) by direct or central vasodilatory mechanisms but little is known about the direct effects of these drugs on placental functions.
-
effect of antihypertensive therapy with alpha methyldopa on levels of Angiogenic factors in pregnancies with hypertensive disorders
PLOS ONE, 2008Co-Authors: Asma Khalil, Shanthi Muttukrishna, K Harrington, Eric JauniauxAbstract:Background: Antihypertensive drugs are believed to lower blood pressure in pre-eclampsia by direct or central vasodilatory mechanisms. However, they could also act by decreasing production of anti-Angiogenic Proteins involved in the pathophysiology of hypertension and proteinuria in pre-eclampsia (PE). The aim of our study was to evaluate the impact of antihypertensive therapy with alpha methyldopa on maternal circulating levels and placental production of soluble fms-like tyrosine kinase 1 (sFlt-1), soluble endoglin (sEng), vascular endothelial growth factor (VEGF) and placental growth factor (PlGF) in hypertensive disorders of pregnancy. Methodology/Principal Findings: In a study conducted at University College Hospital and the Homerton University Hospital in London, we recruited 51 women with PE, 29 with gestational hypertension (GH), and 80 matched normotensive controls. Eight (16%) of the women with PE had severe disease. Placental samples were obtained from a further 48 women (14 PE, 10 GH and 24 matched controls). Serum levels of Angiogenic factors were measured before and 24–48 hours after commencing antihypertensive therapy with alpha methyldopa for clinical indications. The same parameters were measured in placental extracts. In both PE (P,0.0001) and GH (P,0.05), serum sFlt-1 was increased and PlGF reduced at all gestations (P,0.001) compared to controls. Serum sEng levels were also increased in PE. Placental concentration of sFlt-1 and sEng was significantly higher in women with PE compared to controls and women with GH (P,0.0001). The concentration of PlGF was significantly lower in the placental tissue of women with PE compared to GH (P=0.008). Antihypertensive treatment was associated with a significant fall in serum and placental content of sFlt1 and sEng in PE only. Conclusions: Our data suggest that alpha methyldopa may have a specific effect on placental and/or endothelial cell function in pre-eclampsia patients, altering Angiogenic Proteins.
Birgitte Hoier - One of the best experts on this subject based on the ideXlab platform.
-
early time course of change in Angiogenic Proteins in human skeletal muscle and vascular cells with endurance training
Scandinavian Journal of Medicine & Science in Sports, 2020Co-Authors: Birgitte Hoier, Karina Olsen, Dorte Jessing Agerby Hanskov, Maria Monster Jorgensen, Liselotte Rothmann Norup, Ylva HellstenAbstract:Angiogenic, mitochondrial, and related transcriptional Proteins were assessed in human skeletal muscle and isolated vascular cells during the early phase of endurance training. Thigh muscle biopsies were obtained in healthy young subjects, after one acute bout (n = 9) and after 3, 5, 7, and 14 days (n = 9) of cycle ergometer training. Whole muscle homogenates were analyzed for Angiogenic, mitochondrial, and regulatory mRNA and protein levels. Angiogenic Proteins were determined in muscle-derived endothelial cells and pericytes sorted by fluorescence-activated cell sorting. Acute exercise induced an increase in whole muscle mRNA of peroxisome proliferator-activated receptor gamma coactivator 1α (4.5-fold; P = .002) and vascular endothelial growth factor (VEGF) (2.4-fold; P = .001) at 2 hours post. After 14 days of training, there was an increase in CD31 protein (63%; P = .010) in whole muscle indicating capillary growth. There was also an increase in muscle VEGF receptor 2 (VEGFR2) (1.5-fold; P = .013), in OXPHOS Proteins (complex I, II, IV, V; 1.4- to 1.9-fold; P < .05) after 14 days of training and an increase in estrogen-related receptorα protein (1.5-fold; P = .039) at 14 days compared to 5 days of training. Both endothelial cells and pericytes expressed VEGF and other Angiogenic factors at the protein level but with a distinctively lower expression of VEGFR2 and thrombospondin-1 (TSP-1) in pericytes. The findings illustrate that initiation of capillary and mitochondrial adaptations occurs within 14 days of training and suggest that sustained changes in Angiogenic Proteins including VEGF and TSP-1 are moderate in whole muscle and vascular cells.