The Experts below are selected from a list of 58578 Experts worldwide ranked by ideXlab platform

Peter Braude - One of the best experts on this subject based on the ideXlab platform.

  • derivation and propagation of human embryonic stem cell lines from frozen embryos in an Animal Product free environment
    Nature Protocols, 2012
    Co-Authors: Emma Stephenson, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Laureen Jacquet, Cristian Miere, Yaser Dajani, Peter Braude
    Abstract:

    Derivation and propagation of human embryonic stem cell lines from frozen embryos in an Animal Product–free environment

  • derivation and propagation of human embryonic stem cell lines from frozen embryos in an Animal Product free environment
    Nature Protocols, 2012
    Co-Authors: Emma Stephenson, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Laureen Jacquet, Cristian Miere, Yaser Dajani, Peter Braude
    Abstract:

    The protocols described here are comprehensive instructions for deriving human embryonic stem (hES) cell lines in xeno-free conditions from cryopreserved embryos. Details are included for propagation, cryopreservation and characterization. Initial derivation is on feeder cells and is followed by adaptation to a feeder-free environment; competent technicians can perform these simplified methods easily. From derivation to cryopreservation of fully characterized initial stocks takes 3-4 months. These protocols served as the basis for standard operating procedures (SOPs), with both operational and technical components, that we set to meet good manufacturing practice (GMP) and UK regulatory body requirements for derivation of clinical-grade cells. As such, these SOPs are currently used in our current GMP compliant facility to derive hES cell lines ab initio, in an Animal Product-free environment; these lines are suitable for research and potentially for clinical use in cell therapy. So far, we have derived eight clinical-grade lines, which will be freely available to the scientific community after submission/accession to the UK Stem Cell Bank.

Emma Stephenson - One of the best experts on this subject based on the ideXlab platform.

  • generation of kcl026 research grade human embryonic stem cell line carrying a mutation in smn1 gene
    Stem Cell Research, 2016
    Co-Authors: Heema Hewitson, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Emma Stephenson, Dusko Ilic
    Abstract:

    The KCL026 human embryonic stem cell line was derived from an embryo donated for research that carried a mutation in the SMN1 gene encoding survival of motor neuron 1, telomeric (exons 7 and 8 deletion). Mutations in this gene are associated with spinal muscular atrophy. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an Animal Product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.

  • derivation and propagation of human embryonic stem cell lines from frozen embryos in an Animal Product free environment
    Nature Protocols, 2012
    Co-Authors: Emma Stephenson, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Laureen Jacquet, Cristian Miere, Yaser Dajani, Peter Braude
    Abstract:

    Derivation and propagation of human embryonic stem cell lines from frozen embryos in an Animal Product–free environment

  • derivation and propagation of human embryonic stem cell lines from frozen embryos in an Animal Product free environment
    Nature Protocols, 2012
    Co-Authors: Emma Stephenson, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Laureen Jacquet, Cristian Miere, Yaser Dajani, Peter Braude
    Abstract:

    The protocols described here are comprehensive instructions for deriving human embryonic stem (hES) cell lines in xeno-free conditions from cryopreserved embryos. Details are included for propagation, cryopreservation and characterization. Initial derivation is on feeder cells and is followed by adaptation to a feeder-free environment; competent technicians can perform these simplified methods easily. From derivation to cryopreservation of fully characterized initial stocks takes 3-4 months. These protocols served as the basis for standard operating procedures (SOPs), with both operational and technical components, that we set to meet good manufacturing practice (GMP) and UK regulatory body requirements for derivation of clinical-grade cells. As such, these SOPs are currently used in our current GMP compliant facility to derive hES cell lines ab initio, in an Animal Product-free environment; these lines are suitable for research and potentially for clinical use in cell therapy. So far, we have derived eight clinical-grade lines, which will be freely available to the scientific community after submission/accession to the UK Stem Cell Bank.

Victoria Wood - One of the best experts on this subject based on the ideXlab platform.

  • generation of kcl026 research grade human embryonic stem cell line carrying a mutation in smn1 gene
    Stem Cell Research, 2016
    Co-Authors: Heema Hewitson, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Emma Stephenson, Dusko Ilic
    Abstract:

    The KCL026 human embryonic stem cell line was derived from an embryo donated for research that carried a mutation in the SMN1 gene encoding survival of motor neuron 1, telomeric (exons 7 and 8 deletion). Mutations in this gene are associated with spinal muscular atrophy. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an Animal Product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.

  • derivation and propagation of human embryonic stem cell lines from frozen embryos in an Animal Product free environment
    Nature Protocols, 2012
    Co-Authors: Emma Stephenson, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Laureen Jacquet, Cristian Miere, Yaser Dajani, Peter Braude
    Abstract:

    Derivation and propagation of human embryonic stem cell lines from frozen embryos in an Animal Product–free environment

  • derivation and propagation of human embryonic stem cell lines from frozen embryos in an Animal Product free environment
    Nature Protocols, 2012
    Co-Authors: Emma Stephenson, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Laureen Jacquet, Cristian Miere, Yaser Dajani, Peter Braude
    Abstract:

    The protocols described here are comprehensive instructions for deriving human embryonic stem (hES) cell lines in xeno-free conditions from cryopreserved embryos. Details are included for propagation, cryopreservation and characterization. Initial derivation is on feeder cells and is followed by adaptation to a feeder-free environment; competent technicians can perform these simplified methods easily. From derivation to cryopreservation of fully characterized initial stocks takes 3-4 months. These protocols served as the basis for standard operating procedures (SOPs), with both operational and technical components, that we set to meet good manufacturing practice (GMP) and UK regulatory body requirements for derivation of clinical-grade cells. As such, these SOPs are currently used in our current GMP compliant facility to derive hES cell lines ab initio, in an Animal Product-free environment; these lines are suitable for research and potentially for clinical use in cell therapy. So far, we have derived eight clinical-grade lines, which will be freely available to the scientific community after submission/accession to the UK Stem Cell Bank.

Neli Kadeva - One of the best experts on this subject based on the ideXlab platform.

  • generation of kcl026 research grade human embryonic stem cell line carrying a mutation in smn1 gene
    Stem Cell Research, 2016
    Co-Authors: Heema Hewitson, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Emma Stephenson, Dusko Ilic
    Abstract:

    The KCL026 human embryonic stem cell line was derived from an embryo donated for research that carried a mutation in the SMN1 gene encoding survival of motor neuron 1, telomeric (exons 7 and 8 deletion). Mutations in this gene are associated with spinal muscular atrophy. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an Animal Product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.

  • derivation and propagation of human embryonic stem cell lines from frozen embryos in an Animal Product free environment
    Nature Protocols, 2012
    Co-Authors: Emma Stephenson, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Laureen Jacquet, Cristian Miere, Yaser Dajani, Peter Braude
    Abstract:

    Derivation and propagation of human embryonic stem cell lines from frozen embryos in an Animal Product–free environment

  • derivation and propagation of human embryonic stem cell lines from frozen embryos in an Animal Product free environment
    Nature Protocols, 2012
    Co-Authors: Emma Stephenson, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Laureen Jacquet, Cristian Miere, Yaser Dajani, Peter Braude
    Abstract:

    The protocols described here are comprehensive instructions for deriving human embryonic stem (hES) cell lines in xeno-free conditions from cryopreserved embryos. Details are included for propagation, cryopreservation and characterization. Initial derivation is on feeder cells and is followed by adaptation to a feeder-free environment; competent technicians can perform these simplified methods easily. From derivation to cryopreservation of fully characterized initial stocks takes 3-4 months. These protocols served as the basis for standard operating procedures (SOPs), with both operational and technical components, that we set to meet good manufacturing practice (GMP) and UK regulatory body requirements for derivation of clinical-grade cells. As such, these SOPs are currently used in our current GMP compliant facility to derive hES cell lines ab initio, in an Animal Product-free environment; these lines are suitable for research and potentially for clinical use in cell therapy. So far, we have derived eight clinical-grade lines, which will be freely available to the scientific community after submission/accession to the UK Stem Cell Bank.

Glenda Cornwell - One of the best experts on this subject based on the ideXlab platform.

  • generation of kcl026 research grade human embryonic stem cell line carrying a mutation in smn1 gene
    Stem Cell Research, 2016
    Co-Authors: Heema Hewitson, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Emma Stephenson, Dusko Ilic
    Abstract:

    The KCL026 human embryonic stem cell line was derived from an embryo donated for research that carried a mutation in the SMN1 gene encoding survival of motor neuron 1, telomeric (exons 7 and 8 deletion). Mutations in this gene are associated with spinal muscular atrophy. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an Animal Product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.

  • derivation and propagation of human embryonic stem cell lines from frozen embryos in an Animal Product free environment
    Nature Protocols, 2012
    Co-Authors: Emma Stephenson, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Laureen Jacquet, Cristian Miere, Yaser Dajani, Peter Braude
    Abstract:

    Derivation and propagation of human embryonic stem cell lines from frozen embryos in an Animal Product–free environment

  • derivation and propagation of human embryonic stem cell lines from frozen embryos in an Animal Product free environment
    Nature Protocols, 2012
    Co-Authors: Emma Stephenson, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Laureen Jacquet, Cristian Miere, Yaser Dajani, Peter Braude
    Abstract:

    The protocols described here are comprehensive instructions for deriving human embryonic stem (hES) cell lines in xeno-free conditions from cryopreserved embryos. Details are included for propagation, cryopreservation and characterization. Initial derivation is on feeder cells and is followed by adaptation to a feeder-free environment; competent technicians can perform these simplified methods easily. From derivation to cryopreservation of fully characterized initial stocks takes 3-4 months. These protocols served as the basis for standard operating procedures (SOPs), with both operational and technical components, that we set to meet good manufacturing practice (GMP) and UK regulatory body requirements for derivation of clinical-grade cells. As such, these SOPs are currently used in our current GMP compliant facility to derive hES cell lines ab initio, in an Animal Product-free environment; these lines are suitable for research and potentially for clinical use in cell therapy. So far, we have derived eight clinical-grade lines, which will be freely available to the scientific community after submission/accession to the UK Stem Cell Bank.