The Experts below are selected from a list of 216 Experts worldwide ranked by ideXlab platform
Mingren Fuh - One of the best experts on this subject based on the ideXlab platform.
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determination of sulfonamides in Animal Tissues by modified quechers and liquid chromatography tandem mass spectrometry
Talanta, 2017Co-Authors: Chinghsuan Wen, Shuling Lin, Mingren FuhAbstract:Abstract In this study, the salting-out solvent extraction and dispersive solid-phase extraction (dSPE) clean-up steps in QuEChERS (quick, easy, cheap, effective, rugged, and safe) method were optimized to reduce matrix effect and efficiently extract target sulfonamides from a variety of edible Animal Tissues. The extracted sulfonamides were then analyzed using liquid chromatography tandem mass spectrometry (LC-MS/MS). Good extraction recoveries (74.0−100.3% in five different sources of Animal Tissues; n=3) with acceptable matrix effect (
Kaname Tsutsumiuchi - One of the best experts on this subject based on the ideXlab platform.
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determination of avoparcin in Animal Tissues and milk using lc esi ms ms and tandem spe
Journal of Separation Science, 2008Co-Authors: Koichi Inoue, Yasuomi Mizuno, Yukiko Yoshimi, Mari Nunome, Tomoaki Hino, Kaname TsutsumiuchiAbstract:A highly sensitive and selective method using LC-ESI-MS/MS and tandem-SPE was developed to detect trace amounts of avoparcin (AV) antibiotics in Animal Tissues and milk. Data acquisition using MS/MS was achieved by applying multiple reaction monitoring of the product ions of [M + 3H] 3+ and the major product ions of AV-α and -β at m/z 637 → 86/113/130 and m/z 649 → 86/113/130 in ESI(+) mode. The calculated instrumental LODs were 3 ng/mL. The sample preparation was described that the extraction using 5% TFA and the tandem-SPE with an ion-exchange (SAX) and Inert-Sep C18-A cartridge clean-up enable us to determine AV in samples. Ion suppression was decreased by concentration rates of each sample solution. These SPE concentration levels could be used to detect quantities of 5 ppb (milk), 10 ppb (beef), and 25 ppb (chicken muscle and liver). The matrix matching calibration graphs obtained for both AV-a (r >0.996) and -β (r >0.998) from Animal Tissues and milk were linear over the calibration ranges. AV recovery from samples was higher than 73.3% and the RSD was less than 12.0% (n = 5).
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Determination of avoparcin in Animal Tissues and milk using LC‐ESI‐MS/MS and tandem‐SPE
Journal of Separation Science, 2008Co-Authors: Koichi Inoue, Yasuomi Mizuno, Yukiko Yoshimi, Mari Nunome, Tomoaki Hino, Kaname TsutsumiuchiAbstract:A highly sensitive and selective method using LC-ESI-MS/MS and tandem-SPE was developed to detect trace amounts of avoparcin (AV) antibiotics in Animal Tissues and milk. Data acquisition using MS/MS was achieved by applying multiple reaction monitoring of the product ions of [M + 3H] 3+ and the major product ions of AV-α and -β at m/z 637 → 86/113/130 and m/z 649 → 86/113/130 in ESI(+) mode. The calculated instrumental LODs were 3 ng/mL. The sample preparation was described that the extraction using 5% TFA and the tandem-SPE with an ion-exchange (SAX) and Inert-Sep C18-A cartridge clean-up enable us to determine AV in samples. Ion suppression was decreased by concentration rates of each sample solution. These SPE concentration levels could be used to detect quantities of 5 ppb (milk), 10 ppb (beef), and 25 ppb (chicken muscle and liver). The matrix matching calibration graphs obtained for both AV-a (r >0.996) and -β (r >0.998) from Animal Tissues and milk were linear over the calibration ranges. AV recovery from samples was higher than 73.3% and the RSD was less than 12.0% (n = 5).
Indrajit D. Desai - One of the best experts on this subject based on the ideXlab platform.
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VITAMIN E ANALYSIS METHODS FOR Animal Tissues
Methods in Enzymology, 2004Co-Authors: Indrajit D. DesaiAbstract:Publisher Summary A variety of methods have been developed by various investigators for the analysis of vitamin E in Animal Tissues, but many of the earlier procedures involving column chromotography and gas–liquid chromatography are rather complicated and time consuming. The most commonly used methods are based on the saponification and solvent extraction of lipids, the removal or destruction of interfering substances, and the determination of tocopherol spectrophotometricaily or spectrofluorometrically. The methods chosen are for common Animal Tissues, such as blood and organ Tissues. A high-pressure liquid chromatography (HPLC) method for vitamin E has been introduced as a method of choice, but HPLC equipment is expensive and not readily available in every laboratory. The standard colorimetric and fluorometric methods are easily carried out using common laboratory equipment and are often adequate for routine analysis of vitamin E in Animal Tissues for biological research and for clinical testing. For more sophisticated and sensitive detection of commonly occurring α-tocopherol along with trace amounts of other forms of tocopherol and tocotrienol in Animal Tissues, the HPLC method is highly recommended.
Chinghsuan Wen - One of the best experts on this subject based on the ideXlab platform.
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determination of sulfonamides in Animal Tissues by modified quechers and liquid chromatography tandem mass spectrometry
Talanta, 2017Co-Authors: Chinghsuan Wen, Shuling Lin, Mingren FuhAbstract:Abstract In this study, the salting-out solvent extraction and dispersive solid-phase extraction (dSPE) clean-up steps in QuEChERS (quick, easy, cheap, effective, rugged, and safe) method were optimized to reduce matrix effect and efficiently extract target sulfonamides from a variety of edible Animal Tissues. The extracted sulfonamides were then analyzed using liquid chromatography tandem mass spectrometry (LC-MS/MS). Good extraction recoveries (74.0−100.3% in five different sources of Animal Tissues; n=3) with acceptable matrix effect (
Jie Han - One of the best experts on this subject based on the ideXlab platform.
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Determination of sulphonamides in Animal Tissues by high performance liquid chromatography with pre-column derivatization of 9-fluorenylmethyl chloroformate
Journal of separation science, 2007Co-Authors: Qiong-hui Zou, Meng-xia Xie, Xiang-feng Wang, Yuan Liu, Jin Wang, Jia Song, Hui Gao, Jie HanAbstract:A novel approach for simultaneous determination of 12 sulphonamides (sulphadiazine, sulphamethazine, sulphathiazole, sulphadimethoxine, sulphamerazine, sulphapyridine, sulphamethoxazole, suphamethizole, sulphaquinoxaline, sulphameter, sulphamonomethoxine, and sulphachloropyridazine) in Animal Tissues (swine muscle and liver, chicken muscle, beef muscle) by HPLC with UV detection has been developed. A pre-column derivatization of the sulphonamide compounds with 9-fluorenylmethyl chloroformate (FMOC-Cl) has been proposed and the reaction conditions have been optimized. The FMOC-sulphonamide derivatives were purified by SPE with silica gel as solid support prior to HPLC separation. The limits of detection for the sulphonamide compounds were greatly improved after the derivatization and purification step for the derivatives. Sulphonamide residues in Animal Tissues were extracted by acetonitrile and purified by solid phase extraction with C(18) as the solid support. The method developed has high sensitivity and good repeatability, and the average recoveries for most of the sulphonamides at various spiking levels were above 70% with relative standard deviations below 13.7%. The limits of detection for most sulphonamides can reach 3-5 microg/kg.