The Experts below are selected from a list of 1245 Experts worldwide ranked by ideXlab platform
Rana Khalife - One of the best experts on this subject based on the ideXlab platform.
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The pro-apoptotic effect of a Terpene-rich Annona Cherimola leaf extract on leukemic cell lines.
BMC complementary and alternative medicine, 2019Co-Authors: Carl Ammoury, Tony Haykal, Peter Nasr, Mohammad Hassan Hodroj, Robin I. Taleb, Rita Sarkis, Maria Younes, Marianne El Khoury, Marilyne Sily, Rana KhalifeAbstract:The edible fruit Annona Cherimola has previously shown many nutritional and medicinal properties. The current study evaluates the anti-cancer and anti-proliferative properties of Annona Cherimola ethanolic leaf extract (AELE) on Acute Myeloid Leukemia (AML) cell lines cultured in vitro (Monomac-1 and KG-1). The anti-proliferative effect of A. Cherimola ethanolic leaf extract was evaluated via cell viability assay. Its pro-apoptotic effect was assessed through Cell Death ELISA and dual Annexin V/PI staining. To further investigate the molecular mechanism by which the extract promoted apoptosis and inhibited the proliferation of the AML cells used, apoptotic protein expression was determined through western blots. Extract composition was elucidated by Gas Chromatography-Mass Spectrometry (GC-MS). Our results showed that the treatment with A. Cherimola ethanolic leaf extract exhibited an inhibitory effect on the proliferation of both cancer cell lines used in a dose- and time-dependent manner, with no toxic effects on normal mononuclear cells (MNCs) isolated from human bone marrow. This effect was mediated by DNA fragmentation and apoptosis, as revealed by Cell Death ELISA and dual Annexin V/PI staining. Western blot analysis revealed a Bax/Bcl2 dependent mechanism of apoptosis, as well as PARP cleavage, confirming the apoptotic results observed previously. These effects may be attributed to the presence of terpenes which constitute a large component of the leafy extract, as revealed via GC-MS. All the data presented in our study show that the terpene-rich A. Cherimola ethanolic leaf extract exhibits an anti-proliferative and pro-apoptotic effect on the AML cell lines used.
Fang Rong Chang - One of the best experts on this subject based on the ideXlab platform.
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Four alkaloids from Annona Cherimola.
Phytochemistry, 2001Co-Authors: Chung-yi Chen, Fang Rong Chang, Wen Bin PanAbstract:Four alkaloids, annocherine A, annocherine B, cherianoine, and romucosine H, along with one known alkaloid, artabonatine B, were isolated from the MeOH extract of the stems of Annona Cherimola. Their structures were identified on the basis of both analysis of their spectral data and from chemical evidence.
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Aromin-A, an Annonaceous acetogenin from Annona Cherimola.
Phytochemistry, 1999Co-Authors: Chung-yi Chen, Fang Rong Chang, Hui Fen ChiuAbstract:Chromatographic fractionation of a MeOH extract from the stems of Annona Cherimola led to the isolation of a new non-adjacent, bis-THF ring acetogenin (one THF ring being at C-4 and the other at C-16), aromin-A (1) along with the known cytotoxic acetogenin squamocin (2). The structures of these isolates were established by means of mass spectrometry and spectroscopic techniques.
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Amides from stems of Annona Cherimola
Phytochemistry, 1998Co-Authors: Chung-yi Chen, Fang Rong Chang, Hsin Fu YenAbstract:Abstract Four new amides, N - cis -caffeoyltyramine, dihydro-feruloyltyramine, N - trans -feruloylmethoxytyramine and N - cis -feruloylmethoxytyramine, along with two known amides, N - p -coumaroyltyramine and N - cis -feruloyltyramine, were isolated from the fresh stem parts of Annona Cherimola . Their structures were characterized and identified by spectral analysis.
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Cherinonaine, a novel dimeric amide from the stems of Annona Cherimola
Tetrahedron Letters, 1998Co-Authors: Chung-yi Chen, Fang Rong ChangAbstract:Abstract Cherinonaine ( 1 ), a novel Annona dimeric amide, has been isolated from Annona Cherimola , and its structure was determined on the basis of spectroscopic analysis. Amide 1 consists a unique ether bridge between two monomeric amides which are composed from ferulic acid and 6-methoxyl-7-hydroxyl amphetamine.
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LIGNANS AND KAURANES FROM THE STEMS OF Annona Cherimola
Journal of the Chinese Chemical Society, 1998Co-Authors: Chung-yi Chen, Fang Rong ChangAbstract:Four lignans and six kauranes, (+)-epi-syringaresinol (1), annocherin A (16β-hydroxy-17,19-diacetoxy-ent-kaurane) (2), (+)-syringaresinol (3), (+)-dia-syringaresinol (4), liriodendrin[(+)-syringaresinol-di-O-β-D-glucopyranoside] (5), 16α-hydro-17-acetoxy-ent-kauran-19-oic acid (6), 16β-hydro-17-hydroxy-ent-kauran-19-al (7), 16α-hydro-17-hydroxy-ent-kauran-19-al (8), 16β-hydro-17-hydroxy-ent-kauran-19-oic acid (9) and 16α-hydro-17-hydroxy-ent-kauran-19-oic acid (10) were isolated from the stems of Annona Cherimola. Among them, 1 is obtained for the first time from natural sources and 2 is a new compound. The structures of these compounds were characterized and identified by spectral analyses.
Chung-yi Chen - One of the best experts on this subject based on the ideXlab platform.
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annocherine c a new c α hydroxy benzylisoquinoline and other constituents from the leaves of Annona Cherimola
Journal of The Chinese Chemical Society, 2001Co-Authors: Chung-yi ChenAbstract:A new C-α hydroxy benzylisoquinoline, annocherine C (1), along with nine teen compounds including six alkaloids, liriodenine (2), oxoxylopine (3), oxonantenine (4), (-)-asimilobine (5), (-)-xylopine (6) and (-)-anolobine (7); four steroids, β-sitosterol, stigmasterol, β-sitosteryl-D-glucoside and stigmasteryl-D-glucoside and nine benzenoids, methylparabene, vanillin, trans-methyl p-coumarate, cis -methyl p-coumarate, p-hydroxybenzadehyde, p-hydroxybenzoic acid, 4-methoxybenzoic acid methylester, methyl vanillate and p-dihydrocoumaric acid, were isolated from the leaves of Annona Cherimola. These compounds were identified by spectral analysis.
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Annocherine C, A New C‐α Hydroxy Benzylisoquinoline and Other Constituents from the Leaves of Annona Cherimola
Journal of the Chinese Chemical Society, 2001Co-Authors: Chung-yi ChenAbstract:A new C-α hydroxy benzylisoquinoline, annocherine C (1), along with nine teen compounds including six alkaloids, liriodenine (2), oxoxylopine (3), oxonantenine (4), (-)-asimilobine (5), (-)-xylopine (6) and (-)-anolobine (7); four steroids, β-sitosterol, stigmasterol, β-sitosteryl-D-glucoside and stigmasteryl-D-glucoside and nine benzenoids, methylparabene, vanillin, trans-methyl p-coumarate, cis -methyl p-coumarate, p-hydroxybenzadehyde, p-hydroxybenzoic acid, 4-methoxybenzoic acid methylester, methyl vanillate and p-dihydrocoumaric acid, were isolated from the leaves of Annona Cherimola. These compounds were identified by spectral analysis.
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Four alkaloids from Annona Cherimola.
Phytochemistry, 2001Co-Authors: Chung-yi Chen, Fang Rong Chang, Wen Bin PanAbstract:Four alkaloids, annocherine A, annocherine B, cherianoine, and romucosine H, along with one known alkaloid, artabonatine B, were isolated from the MeOH extract of the stems of Annona Cherimola. Their structures were identified on the basis of both analysis of their spectral data and from chemical evidence.
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Aromin-A, an Annonaceous acetogenin from Annona Cherimola.
Phytochemistry, 1999Co-Authors: Chung-yi Chen, Fang Rong Chang, Hui Fen ChiuAbstract:Chromatographic fractionation of a MeOH extract from the stems of Annona Cherimola led to the isolation of a new non-adjacent, bis-THF ring acetogenin (one THF ring being at C-4 and the other at C-16), aromin-A (1) along with the known cytotoxic acetogenin squamocin (2). The structures of these isolates were established by means of mass spectrometry and spectroscopic techniques.
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Amides from stems of Annona Cherimola
Phytochemistry, 1998Co-Authors: Chung-yi Chen, Fang Rong Chang, Hsin Fu YenAbstract:Abstract Four new amides, N - cis -caffeoyltyramine, dihydro-feruloyltyramine, N - trans -feruloylmethoxytyramine and N - cis -feruloylmethoxytyramine, along with two known amides, N - p -coumaroyltyramine and N - cis -feruloyltyramine, were isolated from the fresh stem parts of Annona Cherimola . Their structures were characterized and identified by spectral analysis.
Carl Ammoury - One of the best experts on this subject based on the ideXlab platform.
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The pro-apoptotic effect of a Terpene-rich Annona Cherimola leaf extract on leukemic cell lines.
BMC complementary and alternative medicine, 2019Co-Authors: Carl Ammoury, Tony Haykal, Peter Nasr, Mohammad Hassan Hodroj, Robin I. Taleb, Rita Sarkis, Maria Younes, Marianne El Khoury, Marilyne Sily, Rana KhalifeAbstract:The edible fruit Annona Cherimola has previously shown many nutritional and medicinal properties. The current study evaluates the anti-cancer and anti-proliferative properties of Annona Cherimola ethanolic leaf extract (AELE) on Acute Myeloid Leukemia (AML) cell lines cultured in vitro (Monomac-1 and KG-1). The anti-proliferative effect of A. Cherimola ethanolic leaf extract was evaluated via cell viability assay. Its pro-apoptotic effect was assessed through Cell Death ELISA and dual Annexin V/PI staining. To further investigate the molecular mechanism by which the extract promoted apoptosis and inhibited the proliferation of the AML cells used, apoptotic protein expression was determined through western blots. Extract composition was elucidated by Gas Chromatography-Mass Spectrometry (GC-MS). Our results showed that the treatment with A. Cherimola ethanolic leaf extract exhibited an inhibitory effect on the proliferation of both cancer cell lines used in a dose- and time-dependent manner, with no toxic effects on normal mononuclear cells (MNCs) isolated from human bone marrow. This effect was mediated by DNA fragmentation and apoptosis, as revealed by Cell Death ELISA and dual Annexin V/PI staining. Western blot analysis revealed a Bax/Bcl2 dependent mechanism of apoptosis, as well as PARP cleavage, confirming the apoptotic results observed previously. These effects may be attributed to the presence of terpenes which constitute a large component of the leafy extract, as revealed via GC-MS. All the data presented in our study show that the terpene-rich A. Cherimola ethanolic leaf extract exhibits an anti-proliferative and pro-apoptotic effect on the AML cell lines used.
Tony Haykal - One of the best experts on this subject based on the ideXlab platform.
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The pro-apoptotic effect of a Terpene-rich Annona Cherimola leaf extract on leukemic cell lines.
BMC complementary and alternative medicine, 2019Co-Authors: Carl Ammoury, Tony Haykal, Peter Nasr, Mohammad Hassan Hodroj, Robin I. Taleb, Rita Sarkis, Maria Younes, Marianne El Khoury, Marilyne Sily, Rana KhalifeAbstract:The edible fruit Annona Cherimola has previously shown many nutritional and medicinal properties. The current study evaluates the anti-cancer and anti-proliferative properties of Annona Cherimola ethanolic leaf extract (AELE) on Acute Myeloid Leukemia (AML) cell lines cultured in vitro (Monomac-1 and KG-1). The anti-proliferative effect of A. Cherimola ethanolic leaf extract was evaluated via cell viability assay. Its pro-apoptotic effect was assessed through Cell Death ELISA and dual Annexin V/PI staining. To further investigate the molecular mechanism by which the extract promoted apoptosis and inhibited the proliferation of the AML cells used, apoptotic protein expression was determined through western blots. Extract composition was elucidated by Gas Chromatography-Mass Spectrometry (GC-MS). Our results showed that the treatment with A. Cherimola ethanolic leaf extract exhibited an inhibitory effect on the proliferation of both cancer cell lines used in a dose- and time-dependent manner, with no toxic effects on normal mononuclear cells (MNCs) isolated from human bone marrow. This effect was mediated by DNA fragmentation and apoptosis, as revealed by Cell Death ELISA and dual Annexin V/PI staining. Western blot analysis revealed a Bax/Bcl2 dependent mechanism of apoptosis, as well as PARP cleavage, confirming the apoptotic results observed previously. These effects may be attributed to the presence of terpenes which constitute a large component of the leafy extract, as revealed via GC-MS. All the data presented in our study show that the terpene-rich A. Cherimola ethanolic leaf extract exhibits an anti-proliferative and pro-apoptotic effect on the AML cell lines used.
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Annona Cherimola Seed Extract Activates Extrinsic and Intrinsic Apoptotic Pathways in Leukemic Cells.
Toxins, 2019Co-Authors: Tony Haykal, Peter Nasr, Mohammad Hassan Hodroj, Robin I. Taleb, Rita Sarkis, Marvy Nadine El Moujabber, Sandra RizkAbstract:Annona Cherimola Mill is a large green fruit with black seeds widely known to possess toxic properties due to the presence of Annonaceous acetogenins. The present study investigates the anti-cancer properties of an Annona Cherimola Mill ethanolic seed extract on Acute Myeloid Leukemia (AML) cell lines in vitro and elucidates the underlying cellular mechanism. The anti-proliferative effects of the extract on various AML cell lines and normal mesenchymal cells (MSCs) were assessed using WST-1 viability reagent. The pro-apoptotic effect of the extract was evaluated using Annexin V/PI staining and Cell Death ELISA. The underlying mechanism was deciphered by analyzing the expression of various proteins using western blots. Treatment with an A. Cherimola seed ethanolic extract promotes a dose- and time-dependent inhibition of the proliferation of various AML cell lines, but not MSCs. Positive Annexin V staining, as well as DNA fragmentation, confirm an increase in apoptotic cell death by upregulating the expression of pro-apoptotic proteins which control both intrinsic and extrinsic pathways of apoptosis. GC/MS analysis revealed the presence of phytosterols, in addition to other bioactive compounds. In conclusion, Annona Cherimola Mill seed extract, previously known to possess a potent toxic activity, induces apoptosis in AML cell lines by the activation of both the extrinsic and the intrinsic pathways.
