The Experts below are selected from a list of 258 Experts worldwide ranked by ideXlab platform
Ajeet Kumar Mohanty - One of the best experts on this subject based on the ideXlab platform.
-
proteome data of female Anopheles Stephensi antennae
Data in Brief, 2019Co-Authors: Ajeet Kumar Mohanty, Gourav Dey, Manish Kumar, Sreelakshmi K Sreenivasamurthy, Sandeep Garg, T Keshava S Prasad, Ashwani KumarAbstract:Abstract Antennae of female Anopheles Stephensi mosquitoes were dissected and lysed with 1% SDS. Proteins were extracted using ultra sonication and analyzed on high resolution mass spectrometer. Proteomic data was analyzed using two search algorithms SEQUEST and Mascot, resulting in the identification of 22,729 peptides corresponding to 3262 proteins. These proteins were characterized using different bioinformatics tools. VectorBase resource was used to assign Gene Ontology (GO) terms. Using Biomart tool ortholog information was fetched from the VectorBase database. Raw mass spectrometric data was deposited in ProteomeXchange Consortium via PRIDE partner repository in the public dataset PXD001128. Proteins involved in insecticide resistance and odorant binding were the most abundant in the antennae. The proteins identified in this study could be targeted for developing novel vector control strategy.
-
Susceptibility of wild and colonized Anopheles Stephensi to Plasmodium vivax infection
Malaria journal, 2018Co-Authors: Ajeet Kumar Mohanty, Praveen Balabaskaran Nina, Shuvankar Ballav, Smita Vernekar, Sushma Parkar, Maria D’souza, Wenyun Zuo, Edwin Gomes, Laura Chery, Shripad TuljapurkarAbstract:As much as 80% of global Plasmodium vivax infections occur in South Asia and there is a shortage of direct studies on infectivity of P. vivax in Anopheles Stephensi, the most common urban mosquito carrying human malaria. In this quest, the possible effects of laboratory colonization of mosquitoes on infectivity and development of P. vivax is of interest given that colonized mosquitoes can be genetically less divergent than the field population from which they originated. Patient-derived P. vivax infected blood was fed to age-matched wild and colonized An. Stephensi. Such a comparison requires coordinated availability of same-age wild and colonized mosquito populations. Here, P. vivax infection are studied in colonized An. Stephensi in their 66th–86th generation and fresh field-caught An. Stephensi. Wild mosquitoes were caught as larvae and pupae and allowed to develop into adult mosquitoes in the insectary. Parasite development to oocyst and sporozoite stages were assessed on days 7/8 and 12/13, respectively. While there were batch to batch variations in infectivity of individual patient-derived P. vivax samples, both wild and colonized An. Stephensi were roughly equally susceptible to oocyst stage Plasmodium infection. At the level of sporozoite development, significantly more mosquitoes with sporozoite load of 4+ were seen in wild than in colonized populations. Overall at the level of oocyst development, significant difference was found between the colonized and wild Anopheles Stephensi in their susceptibility to P. vivax. For initial understanding of infections with local strains of P. vivax, colonized Anopheles Stephensi will serve as a good model. For experiments, where high number of sporozoites are necessary, wild mosquitoes provide distinct advantage over the colonized vector populations. Understanding the molecular mechanism modulating this variability between these two populations will be prime area of focus in future studies.
-
Proteome data of Anopheles Stephensi ovary using high-resolution mass spectrometry
Elsevier, 2018Co-Authors: Gourav Dey, Ajeet Kumar Mohanty, Manish Kumar, Sreelakshmi K Sreenivasamurthy, Arun H. Patil, T.s. Keshava Prasad, Ashwani KumarAbstract:This article contains data on the proteins expressed in the ovaries of Anopheles Stephensi, a major vector of malaria in India. Data acquisition was performed using a high-resolution Orbitrap-Velos mass spectrometer. The acquired MS/MS data was searched against An. Stephensi protein database comprising of 11,789 sequences. Overall, 4407 proteins were identified, functional analysis was performed for the identified proteins and a protein-protein interaction map predicted. The data provided here is also related to a published article - “Integrating transcriptomics and proteomics data for accurate assembly and annotation of genomes” (Prasad et al., 2017) [1]
Shripad Tuljapurkar - One of the best experts on this subject based on the ideXlab platform.
-
Susceptibility of wild and colonized Anopheles Stephensi to Plasmodium vivax infection
Malaria journal, 2018Co-Authors: Ajeet Kumar Mohanty, Praveen Balabaskaran Nina, Shuvankar Ballav, Smita Vernekar, Sushma Parkar, Maria D’souza, Wenyun Zuo, Edwin Gomes, Laura Chery, Shripad TuljapurkarAbstract:As much as 80% of global Plasmodium vivax infections occur in South Asia and there is a shortage of direct studies on infectivity of P. vivax in Anopheles Stephensi, the most common urban mosquito carrying human malaria. In this quest, the possible effects of laboratory colonization of mosquitoes on infectivity and development of P. vivax is of interest given that colonized mosquitoes can be genetically less divergent than the field population from which they originated. Patient-derived P. vivax infected blood was fed to age-matched wild and colonized An. Stephensi. Such a comparison requires coordinated availability of same-age wild and colonized mosquito populations. Here, P. vivax infection are studied in colonized An. Stephensi in their 66th–86th generation and fresh field-caught An. Stephensi. Wild mosquitoes were caught as larvae and pupae and allowed to develop into adult mosquitoes in the insectary. Parasite development to oocyst and sporozoite stages were assessed on days 7/8 and 12/13, respectively. While there were batch to batch variations in infectivity of individual patient-derived P. vivax samples, both wild and colonized An. Stephensi were roughly equally susceptible to oocyst stage Plasmodium infection. At the level of sporozoite development, significantly more mosquitoes with sporozoite load of 4+ were seen in wild than in colonized populations. Overall at the level of oocyst development, significant difference was found between the colonized and wild Anopheles Stephensi in their susceptibility to P. vivax. For initial understanding of infections with local strains of P. vivax, colonized Anopheles Stephensi will serve as a good model. For experiments, where high number of sporozoites are necessary, wild mosquitoes provide distinct advantage over the colonized vector populations. Understanding the molecular mechanism modulating this variability between these two populations will be prime area of focus in future studies.
L. Triest - One of the best experts on this subject based on the ideXlab platform.
-
Effect of Ambrosia maritima on Anopheles Stephensi and Aedes aegypti
Journal of ethnopharmacology, 1994Co-Authors: Stanny Geerts, K. Van Blerk, L. TriestAbstract:The toxicity of the molluscicidal plant Ambrosia maritima L. was evaluated against Anopheles Stephensi and Aedes aegypti. In the larvicidal assays a negligible mortality was observed in both species after application of the dried leaves in the water at concentrations up to 2000 mg/l. When the powdered leaves were applied at the surface of the water, however, up to 38% of the larvae of A. Stephensi were killed at 2000 mg/l. Virtually no inhibitory effect on the larval growth of both species was noticed. It can be concluded that Ambrosia maritima has little or no effect on the larvae of A. Stephensi or Ae. aegypti.
Shan Feng-ping - One of the best experts on this subject based on the ideXlab platform.
-
Changes of Gametocyte Activating Factor in the Salivary Glands of Anopheles Stephensi During Growth and Development
Journal of Microbiology, 2007Co-Authors: Shan Feng-pingAbstract:The dynamic changes of gametocyte activating factor(GAF) activity in the salivary gland extracts were compared between non-blood-fed and blood-fed male Anopheles Stephensi post-emergence by in vitro exflagellation assay of Plasmodium berghei in order to investigate the rise and fall of GAF in the salivary glands of Anopheles Stephensi during growth and development.After emergence,the varying tendency of GAF activity in the salivary glands of non-blood-fed mosquitoes was consistent with their growth and development.On the day 0 to day 6 after emergence,GAF activity in the salivary glands of blood-fed mosquitoes was similar to that of non-blood-fed mosquitoes,it declined after blood feeding,and recovered to the original level of pre-feeding on day 14 after emergence.The fall of GAF activity in the anopheline salivary glands after blood feeding may be related to development of mosquito eggs.
Stanny Geerts - One of the best experts on this subject based on the ideXlab platform.
-
Effect of Ambrosia maritima on Anopheles Stephensi and Aedes aegypti
Journal of ethnopharmacology, 1994Co-Authors: Stanny Geerts, K. Van Blerk, L. TriestAbstract:The toxicity of the molluscicidal plant Ambrosia maritima L. was evaluated against Anopheles Stephensi and Aedes aegypti. In the larvicidal assays a negligible mortality was observed in both species after application of the dried leaves in the water at concentrations up to 2000 mg/l. When the powdered leaves were applied at the surface of the water, however, up to 38% of the larvae of A. Stephensi were killed at 2000 mg/l. Virtually no inhibitory effect on the larval growth of both species was noticed. It can be concluded that Ambrosia maritima has little or no effect on the larvae of A. Stephensi or Ae. aegypti.