The Experts below are selected from a list of 312 Experts worldwide ranked by ideXlab platform
James A. North - One of the best experts on this subject based on the ideXlab platform.
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In vitro virucidal activity of selected Anthraquinones and Anthraquinone derivatives
Antiviral Research, 1991Co-Authors: Douglas O. Andersen, Norbert D. Weber, Byron K. Murray, Bronwyn G. Hughes, Steven G. Wood, James A. NorthAbstract:Abstract Anthraquinones and Anthraquinone derivatives were characterized for their antiviral and virucidal activities against viruses representing several taxonomic groups. One of these compounds, hypericin, had activity against vesicular stomatitis virus, herpes simplex virus types 1 and 2, parainfluenza virus, and vaccinia virus (from 0.5 to 3.8 log10 reductions in infectivity) at concentrations of less than 1 μg/ml as determined by a direct pre-infection incubation assay. Human rhinovirus was not sensitive to hypericin at concentrations up to 10 μg/ml. Addition of small amounts of Tween-80 to solutions containing hypericin enhanced, by up to 2.6 log10, hypericin's virucidal activity. Anthraquinones and Anthraquinone derivatives with the hydroxyl and alkyl substitution pattern of emodin (i.e. emodin, emodin anthrone, emodin bianthrone and hypericin) were active against the enveloped viruses tested. The following general pattern of activity was found: hypericin > emodin bianthrone > emodin anthrone > emodin. Chrysophanic acid, aloe-emodin, and sennosides A and B did not possess activity against any of the viruses tested.
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In vitro virucidal activity of selected Anthraquinones and Anthraquinone derivatives
Antiviral research, 1991Co-Authors: Douglas O. Andersen, Norbert D. Weber, Byron K. Murray, Bronwyn G. Hughes, Steven G. Wood, James A. NorthAbstract:Anthraquinones and Anthraquinone derivatives were characterized for their antiviral and virucidal activities against viruses representing several taxonomic groups. One of these compounds, hypericin, had activity against vesicular stomatitis virus, herpes simplex virus types 1 and 2, parainfluenza virus, and vaccinia virus (from 0.5 to 3.8 log10 reductions in infectivity) at concentrations of less than 1 microgram/ml as determined by a direct pre-infection incubation assay. Human rhinovirus was not sensitive to hypericin at concentrations up to 10 micrograms/ml. Addition of small amounts of Tween-80 to solutions containing hypericin enhanced, by up to 2.6 log10, hypericin's virucidal activity. Anthraquinones and Anthraquinone derivatives with the hydroxyl and alkyl substitution pattern of emodin (i.e. emodin, emodin anthrone, emodin bianthrone and hypericin) were active against the enveloped viruses tested. The following general pattern of activity was found: hypericin greater than emodin bianthrone greater than emodin anthrone greater than emodin. Chrysophanic acid, aloe-emodin, and sennosides A and B did not possess activity against any of the viruses tested.
So Youn Won - One of the best experts on this subject based on the ideXlab platform.
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Genome-enabled discovery of Anthraquinone biosynthesis in Senna tora
Nature Communications, 2020Co-Authors: Sang-ho Kang, Ramesh Prasad Pandey, Chang-muk Lee, Joon-soo Sim, Jin-tae Jeong, Beom-soon Choi, Myunghee Jung, Daniel Ginzburg, Kangmei Zhao, So Youn WonAbstract:Anthraquinones are aromatic polyketides and have been used for treating various diseases, but the biosynthetic pathway is unclear. Here, the authors assemble the genome of an Anthraquinone-producing medicinal plant Senna tora and show the evidences that CHS-like genes may be involved in Anthraquinone biosynthesis. Senna tora is a widely used medicinal plant. Its health benefits have been attributed to the large quantity of Anthraquinones, but how they are made in plants remains a mystery. To identify the genes responsible for plant Anthraquinone biosynthesis, we reveal the genome sequence of S. tora at the chromosome level with 526 Mb (96%) assembled into 13 chromosomes. Comparison among related plant species shows that a chalcone synthase-like (CHS-L) gene family has lineage-specifically and rapidly expanded in S. tora . Combining genomics, transcriptomics, metabolomics, and biochemistry, we identify a CHS-L gene contributing to the biosynthesis of Anthraquinones. The S. tora reference genome will accelerate the discovery of biologically active Anthraquinone biosynthesis pathways in medicinal plants.
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genome enabled discovery of Anthraquinone biosynthesis in senna tora
Nature Communications, 2020Co-Authors: Sang-ho Kang, Ramesh Prasad Pandey, Chang-muk Lee, Joon-soo Sim, Jin-tae Jeong, Beom-soon Choi, Myunghee Jung, Daniel Ginzburg, Kangmei Zhao, So Youn WonAbstract:Senna tora is a widely used medicinal plant. Its health benefits have been attributed to the large quantity of Anthraquinones, but how they are made in plants remains a mystery. To identify the genes responsible for plant Anthraquinone biosynthesis, we reveal the genome sequence of S. tora at the chromosome level with 526 Mb (96%) assembled into 13 chromosomes. Comparison among related plant species shows that a chalcone synthase-like (CHS-L) gene family has lineage-specifically and rapidly expanded in S. tora. Combining genomics, transcriptomics, metabolomics, and biochemistry, we identify a CHS-L gene contributing to the biosynthesis of Anthraquinones. The S. tora reference genome will accelerate the discovery of biologically active Anthraquinone biosynthesis pathways in medicinal plants.
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de novo transcriptome sequence of senna tora provides insights into Anthraquinone biosynthesis
PLOS ONE, 2020Co-Authors: Sang-ho Kang, Chang-muk Lee, Joon-soo Sim, So Youn Won, Woohaeng Lee, Sora Han, Soojin Kwon, Jung Sun Kim, Changkug KimAbstract:Senna tora is an annual herb with rich source of Anthraquinones that have tremendous pharmacological properties. However, there is little mention of genetic information for this species, especially regarding the biosynthetic pathways of Anthraquinones. To understand the key genes and regulatory mechanism of Anthraquinone biosynthesis pathways, we performed spatial and temporal transcriptome sequencing of S. tora using short RNA sequencing (RNA-Seq) and long-read isoform sequencing (Iso-Seq) technologies, and generated two unigene sets composed of 118,635 and 39,364, respectively. A comprehensive functional annotation and classification with multiple public databases identified array of genes involved in major secondary metabolite biosynthesis pathways and important transcription factor (TF) families (MYB, MYB-related, AP2/ERF, C2C2-YABBY, and bHLH). Differential expression analysis indicated that the expression level of genes involved in Anthraquinone biosynthetic pathway regulates differently depending on the degree of tissues and seeds development. Furthermore, we identified that the amount of Anthraquinone compounds were greater in late seeds than early ones. In conclusion, these results provide a rich resource for understanding the Anthraquinone metabolism in S. tora.
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genome enabled discovery of Anthraquinone biosynthesis in senna tora
bioRxiv, 2020Co-Authors: Sang-ho Kang, Ramesh Prasad Pandey, Chang-muk Lee, Joon-soo Sim, Jin-tae Jeong, Beom-soon Choi, Myunghee Jung, So Youn Won, Namhoon Kim, Ok Ran LeeAbstract:Senna tora is a widely used medicinal plant. Its health benefits have been attributed to the large quantity of Anthraquinones, but how they are made in plants remains a mystery. To identify the genes responsible for plant Anthraquinone biosynthesis, we sequenced and annotated the genome of S. tora at the chromosome level with contig N50 and super-scaffold N50 of 4.03 Mb and 41.7 Mb. Comparison among related plant species showed that a chalcone synthase-like (CHS-L) gene family has lineage-specifically and rapidly expanded in S. tora. Combining genomics, transcriptomics, metabolomics, and biochemistry, we identified a CHS-L responsible for biosynthesis of Anthraquinones, the first example in plants. The S. tora reference genome will accelerate the discovery of biologically active Anthraquinone biosynthesis pathways in medicinal plants.
Sang-ho Kang - One of the best experts on this subject based on the ideXlab platform.
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Genome-enabled discovery of Anthraquinone biosynthesis in Senna tora
Nature Communications, 2020Co-Authors: Sang-ho Kang, Ramesh Prasad Pandey, Chang-muk Lee, Joon-soo Sim, Jin-tae Jeong, Beom-soon Choi, Myunghee Jung, Daniel Ginzburg, Kangmei Zhao, So Youn WonAbstract:Anthraquinones are aromatic polyketides and have been used for treating various diseases, but the biosynthetic pathway is unclear. Here, the authors assemble the genome of an Anthraquinone-producing medicinal plant Senna tora and show the evidences that CHS-like genes may be involved in Anthraquinone biosynthesis. Senna tora is a widely used medicinal plant. Its health benefits have been attributed to the large quantity of Anthraquinones, but how they are made in plants remains a mystery. To identify the genes responsible for plant Anthraquinone biosynthesis, we reveal the genome sequence of S. tora at the chromosome level with 526 Mb (96%) assembled into 13 chromosomes. Comparison among related plant species shows that a chalcone synthase-like (CHS-L) gene family has lineage-specifically and rapidly expanded in S. tora . Combining genomics, transcriptomics, metabolomics, and biochemistry, we identify a CHS-L gene contributing to the biosynthesis of Anthraquinones. The S. tora reference genome will accelerate the discovery of biologically active Anthraquinone biosynthesis pathways in medicinal plants.
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genome enabled discovery of Anthraquinone biosynthesis in senna tora
Nature Communications, 2020Co-Authors: Sang-ho Kang, Ramesh Prasad Pandey, Chang-muk Lee, Joon-soo Sim, Jin-tae Jeong, Beom-soon Choi, Myunghee Jung, Daniel Ginzburg, Kangmei Zhao, So Youn WonAbstract:Senna tora is a widely used medicinal plant. Its health benefits have been attributed to the large quantity of Anthraquinones, but how they are made in plants remains a mystery. To identify the genes responsible for plant Anthraquinone biosynthesis, we reveal the genome sequence of S. tora at the chromosome level with 526 Mb (96%) assembled into 13 chromosomes. Comparison among related plant species shows that a chalcone synthase-like (CHS-L) gene family has lineage-specifically and rapidly expanded in S. tora. Combining genomics, transcriptomics, metabolomics, and biochemistry, we identify a CHS-L gene contributing to the biosynthesis of Anthraquinones. The S. tora reference genome will accelerate the discovery of biologically active Anthraquinone biosynthesis pathways in medicinal plants.
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de novo transcriptome sequence of senna tora provides insights into Anthraquinone biosynthesis
PLOS ONE, 2020Co-Authors: Sang-ho Kang, Chang-muk Lee, Joon-soo Sim, So Youn Won, Woohaeng Lee, Sora Han, Soojin Kwon, Jung Sun Kim, Changkug KimAbstract:Senna tora is an annual herb with rich source of Anthraquinones that have tremendous pharmacological properties. However, there is little mention of genetic information for this species, especially regarding the biosynthetic pathways of Anthraquinones. To understand the key genes and regulatory mechanism of Anthraquinone biosynthesis pathways, we performed spatial and temporal transcriptome sequencing of S. tora using short RNA sequencing (RNA-Seq) and long-read isoform sequencing (Iso-Seq) technologies, and generated two unigene sets composed of 118,635 and 39,364, respectively. A comprehensive functional annotation and classification with multiple public databases identified array of genes involved in major secondary metabolite biosynthesis pathways and important transcription factor (TF) families (MYB, MYB-related, AP2/ERF, C2C2-YABBY, and bHLH). Differential expression analysis indicated that the expression level of genes involved in Anthraquinone biosynthetic pathway regulates differently depending on the degree of tissues and seeds development. Furthermore, we identified that the amount of Anthraquinone compounds were greater in late seeds than early ones. In conclusion, these results provide a rich resource for understanding the Anthraquinone metabolism in S. tora.
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genome enabled discovery of Anthraquinone biosynthesis in senna tora
bioRxiv, 2020Co-Authors: Sang-ho Kang, Ramesh Prasad Pandey, Chang-muk Lee, Joon-soo Sim, Jin-tae Jeong, Beom-soon Choi, Myunghee Jung, So Youn Won, Namhoon Kim, Ok Ran LeeAbstract:Senna tora is a widely used medicinal plant. Its health benefits have been attributed to the large quantity of Anthraquinones, but how they are made in plants remains a mystery. To identify the genes responsible for plant Anthraquinone biosynthesis, we sequenced and annotated the genome of S. tora at the chromosome level with contig N50 and super-scaffold N50 of 4.03 Mb and 41.7 Mb. Comparison among related plant species showed that a chalcone synthase-like (CHS-L) gene family has lineage-specifically and rapidly expanded in S. tora. Combining genomics, transcriptomics, metabolomics, and biochemistry, we identified a CHS-L responsible for biosynthesis of Anthraquinones, the first example in plants. The S. tora reference genome will accelerate the discovery of biologically active Anthraquinone biosynthesis pathways in medicinal plants.
Douglas O. Andersen - One of the best experts on this subject based on the ideXlab platform.
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In vitro virucidal activity of selected Anthraquinones and Anthraquinone derivatives
Antiviral Research, 1991Co-Authors: Douglas O. Andersen, Norbert D. Weber, Byron K. Murray, Bronwyn G. Hughes, Steven G. Wood, James A. NorthAbstract:Abstract Anthraquinones and Anthraquinone derivatives were characterized for their antiviral and virucidal activities against viruses representing several taxonomic groups. One of these compounds, hypericin, had activity against vesicular stomatitis virus, herpes simplex virus types 1 and 2, parainfluenza virus, and vaccinia virus (from 0.5 to 3.8 log10 reductions in infectivity) at concentrations of less than 1 μg/ml as determined by a direct pre-infection incubation assay. Human rhinovirus was not sensitive to hypericin at concentrations up to 10 μg/ml. Addition of small amounts of Tween-80 to solutions containing hypericin enhanced, by up to 2.6 log10, hypericin's virucidal activity. Anthraquinones and Anthraquinone derivatives with the hydroxyl and alkyl substitution pattern of emodin (i.e. emodin, emodin anthrone, emodin bianthrone and hypericin) were active against the enveloped viruses tested. The following general pattern of activity was found: hypericin > emodin bianthrone > emodin anthrone > emodin. Chrysophanic acid, aloe-emodin, and sennosides A and B did not possess activity against any of the viruses tested.
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In vitro virucidal activity of selected Anthraquinones and Anthraquinone derivatives
Antiviral research, 1991Co-Authors: Douglas O. Andersen, Norbert D. Weber, Byron K. Murray, Bronwyn G. Hughes, Steven G. Wood, James A. NorthAbstract:Anthraquinones and Anthraquinone derivatives were characterized for their antiviral and virucidal activities against viruses representing several taxonomic groups. One of these compounds, hypericin, had activity against vesicular stomatitis virus, herpes simplex virus types 1 and 2, parainfluenza virus, and vaccinia virus (from 0.5 to 3.8 log10 reductions in infectivity) at concentrations of less than 1 microgram/ml as determined by a direct pre-infection incubation assay. Human rhinovirus was not sensitive to hypericin at concentrations up to 10 micrograms/ml. Addition of small amounts of Tween-80 to solutions containing hypericin enhanced, by up to 2.6 log10, hypericin's virucidal activity. Anthraquinones and Anthraquinone derivatives with the hydroxyl and alkyl substitution pattern of emodin (i.e. emodin, emodin anthrone, emodin bianthrone and hypericin) were active against the enveloped viruses tested. The following general pattern of activity was found: hypericin greater than emodin bianthrone greater than emodin anthrone greater than emodin. Chrysophanic acid, aloe-emodin, and sennosides A and B did not possess activity against any of the viruses tested.
Joon-soo Sim - One of the best experts on this subject based on the ideXlab platform.
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Genome-enabled discovery of Anthraquinone biosynthesis in Senna tora
Nature Communications, 2020Co-Authors: Sang-ho Kang, Ramesh Prasad Pandey, Chang-muk Lee, Joon-soo Sim, Jin-tae Jeong, Beom-soon Choi, Myunghee Jung, Daniel Ginzburg, Kangmei Zhao, So Youn WonAbstract:Anthraquinones are aromatic polyketides and have been used for treating various diseases, but the biosynthetic pathway is unclear. Here, the authors assemble the genome of an Anthraquinone-producing medicinal plant Senna tora and show the evidences that CHS-like genes may be involved in Anthraquinone biosynthesis. Senna tora is a widely used medicinal plant. Its health benefits have been attributed to the large quantity of Anthraquinones, but how they are made in plants remains a mystery. To identify the genes responsible for plant Anthraquinone biosynthesis, we reveal the genome sequence of S. tora at the chromosome level with 526 Mb (96%) assembled into 13 chromosomes. Comparison among related plant species shows that a chalcone synthase-like (CHS-L) gene family has lineage-specifically and rapidly expanded in S. tora . Combining genomics, transcriptomics, metabolomics, and biochemistry, we identify a CHS-L gene contributing to the biosynthesis of Anthraquinones. The S. tora reference genome will accelerate the discovery of biologically active Anthraquinone biosynthesis pathways in medicinal plants.
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genome enabled discovery of Anthraquinone biosynthesis in senna tora
Nature Communications, 2020Co-Authors: Sang-ho Kang, Ramesh Prasad Pandey, Chang-muk Lee, Joon-soo Sim, Jin-tae Jeong, Beom-soon Choi, Myunghee Jung, Daniel Ginzburg, Kangmei Zhao, So Youn WonAbstract:Senna tora is a widely used medicinal plant. Its health benefits have been attributed to the large quantity of Anthraquinones, but how they are made in plants remains a mystery. To identify the genes responsible for plant Anthraquinone biosynthesis, we reveal the genome sequence of S. tora at the chromosome level with 526 Mb (96%) assembled into 13 chromosomes. Comparison among related plant species shows that a chalcone synthase-like (CHS-L) gene family has lineage-specifically and rapidly expanded in S. tora. Combining genomics, transcriptomics, metabolomics, and biochemistry, we identify a CHS-L gene contributing to the biosynthesis of Anthraquinones. The S. tora reference genome will accelerate the discovery of biologically active Anthraquinone biosynthesis pathways in medicinal plants.
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de novo transcriptome sequence of senna tora provides insights into Anthraquinone biosynthesis
PLOS ONE, 2020Co-Authors: Sang-ho Kang, Chang-muk Lee, Joon-soo Sim, So Youn Won, Woohaeng Lee, Sora Han, Soojin Kwon, Jung Sun Kim, Changkug KimAbstract:Senna tora is an annual herb with rich source of Anthraquinones that have tremendous pharmacological properties. However, there is little mention of genetic information for this species, especially regarding the biosynthetic pathways of Anthraquinones. To understand the key genes and regulatory mechanism of Anthraquinone biosynthesis pathways, we performed spatial and temporal transcriptome sequencing of S. tora using short RNA sequencing (RNA-Seq) and long-read isoform sequencing (Iso-Seq) technologies, and generated two unigene sets composed of 118,635 and 39,364, respectively. A comprehensive functional annotation and classification with multiple public databases identified array of genes involved in major secondary metabolite biosynthesis pathways and important transcription factor (TF) families (MYB, MYB-related, AP2/ERF, C2C2-YABBY, and bHLH). Differential expression analysis indicated that the expression level of genes involved in Anthraquinone biosynthetic pathway regulates differently depending on the degree of tissues and seeds development. Furthermore, we identified that the amount of Anthraquinone compounds were greater in late seeds than early ones. In conclusion, these results provide a rich resource for understanding the Anthraquinone metabolism in S. tora.
-
genome enabled discovery of Anthraquinone biosynthesis in senna tora
bioRxiv, 2020Co-Authors: Sang-ho Kang, Ramesh Prasad Pandey, Chang-muk Lee, Joon-soo Sim, Jin-tae Jeong, Beom-soon Choi, Myunghee Jung, So Youn Won, Namhoon Kim, Ok Ran LeeAbstract:Senna tora is a widely used medicinal plant. Its health benefits have been attributed to the large quantity of Anthraquinones, but how they are made in plants remains a mystery. To identify the genes responsible for plant Anthraquinone biosynthesis, we sequenced and annotated the genome of S. tora at the chromosome level with contig N50 and super-scaffold N50 of 4.03 Mb and 41.7 Mb. Comparison among related plant species showed that a chalcone synthase-like (CHS-L) gene family has lineage-specifically and rapidly expanded in S. tora. Combining genomics, transcriptomics, metabolomics, and biochemistry, we identified a CHS-L responsible for biosynthesis of Anthraquinones, the first example in plants. The S. tora reference genome will accelerate the discovery of biologically active Anthraquinone biosynthesis pathways in medicinal plants.