The Experts below are selected from a list of 194568 Experts worldwide ranked by ideXlab platform

Laura Lazo - One of the best experts on this subject based on the ideXlab platform.

  • Anamnestic Antibody Response after viral challenge in monkeys immunized with dengue 2 recombinant fusion proteins
    Archives of Virology, 2008
    Co-Authors: Lidice Bernardo, Jorge Martin, Lisset Hermida, Mayling Alvarez, Irina Prado, Carlos López, Rafael Martínez, Rosmari Rodríguez-roche, Aida Zulueta, Laura Lazo
    Abstract:

    The suitability of dengue 2 envelope domain III recombinant fusion proteins [(fusion (PD5) and insertion (PD3) variants)] for inducing functional antibodies and a protective immune Response in nonhuman primates has been reported. However, the evaluation of the Antibody Response after immunization did not correlate with the protection data as measured by viremia detection. Here, we characterized the anamnestic immune Response after viral challenge in monkeys immunized with the dengue 2 recombinant proteins in an attempt to define correlates of protection useful for vaccine studies. Monkeys immunized with PD5 (most protected group) exhibited an earlier increase in the anti-DENV-2 IgM Response after challenge compared to control animals. Hemagglutination-inhibiting (HAI) antibodies were increased significantly earlier in PD5-immunized animals compared to those immunized with PD3. The fully protected monkeys showed the earliest HAI Antibody Response. These results underline the usefulness of the anamnestic Antibody Response for supporting protection data. The induction of an early HAI and IgM Antibody Response after challenge suggest a protective role against dengue virus (DENV) infection in monkeys, supporting their use as correlates of protection in vaccine studies.

  • Anamnestic Antibody Response after viral challenge in monkeys immunized with dengue 2 recombinant fusion proteins
    'Springer Science and Business Media LLC', 2008
    Co-Authors: Lidice Bernardo, Jorge Martin, Lisset Hermida, Mayling Alvarez, Irina Prado, Carlos López, Rafael Martínez, Rosmari Rodríguez-roche, Aida Zulueta, Laura Lazo
    Abstract:

    The suitability of dengue 2 envelope domain III recombinant fusion proteins [(fusion (PD5) and insertion (PD3) variants)] for inducing functional antibodies and a protective immune Response in nonhuman primates has been reported. However, the evaluation of the Antibody Response after immunization did not correlate with the protection data as measured by viremia detection. Here, we characterized the anamnestic immune Response after viral challenge in monkeys immunized with the dengue 2 recombinant proteins in an attempt to define correlates of protection useful for vaccine studies. Monkeys immunized with PD5 (most protected group) exhibited an earlier increase in the anti-DENV-2 IgM Response after challenge compared to control animals. Hemagglutination- inhibiting (HAI) antibodies were increased significantly earlier in PD5-immunized animals compared to those immunized with PD3. The fully protected monkeys showed the earliest HAI Antibody Response. These results underline the usefulness of the anamnestic Antibody Response for supporting protection data. The induction of an early HAI and IgM Antibody Response after challenge suggest a protective role against dengue virus (DENV) infection in monkeys, supporting their use as correlates of protection in vaccine studies. © 2008 Springer-Verlag

Lidice Bernardo - One of the best experts on this subject based on the ideXlab platform.

  • Anamnestic Antibody Response after viral challenge in monkeys immunized with dengue 2 recombinant fusion proteins
    Archives of Virology, 2008
    Co-Authors: Lidice Bernardo, Jorge Martin, Lisset Hermida, Mayling Alvarez, Irina Prado, Carlos López, Rafael Martínez, Rosmari Rodríguez-roche, Aida Zulueta, Laura Lazo
    Abstract:

    The suitability of dengue 2 envelope domain III recombinant fusion proteins [(fusion (PD5) and insertion (PD3) variants)] for inducing functional antibodies and a protective immune Response in nonhuman primates has been reported. However, the evaluation of the Antibody Response after immunization did not correlate with the protection data as measured by viremia detection. Here, we characterized the anamnestic immune Response after viral challenge in monkeys immunized with the dengue 2 recombinant proteins in an attempt to define correlates of protection useful for vaccine studies. Monkeys immunized with PD5 (most protected group) exhibited an earlier increase in the anti-DENV-2 IgM Response after challenge compared to control animals. Hemagglutination-inhibiting (HAI) antibodies were increased significantly earlier in PD5-immunized animals compared to those immunized with PD3. The fully protected monkeys showed the earliest HAI Antibody Response. These results underline the usefulness of the anamnestic Antibody Response for supporting protection data. The induction of an early HAI and IgM Antibody Response after challenge suggest a protective role against dengue virus (DENV) infection in monkeys, supporting their use as correlates of protection in vaccine studies.

  • Anamnestic Antibody Response after viral challenge in monkeys immunized with dengue 2 recombinant fusion proteins
    'Springer Science and Business Media LLC', 2008
    Co-Authors: Lidice Bernardo, Jorge Martin, Lisset Hermida, Mayling Alvarez, Irina Prado, Carlos López, Rafael Martínez, Rosmari Rodríguez-roche, Aida Zulueta, Laura Lazo
    Abstract:

    The suitability of dengue 2 envelope domain III recombinant fusion proteins [(fusion (PD5) and insertion (PD3) variants)] for inducing functional antibodies and a protective immune Response in nonhuman primates has been reported. However, the evaluation of the Antibody Response after immunization did not correlate with the protection data as measured by viremia detection. Here, we characterized the anamnestic immune Response after viral challenge in monkeys immunized with the dengue 2 recombinant proteins in an attempt to define correlates of protection useful for vaccine studies. Monkeys immunized with PD5 (most protected group) exhibited an earlier increase in the anti-DENV-2 IgM Response after challenge compared to control animals. Hemagglutination- inhibiting (HAI) antibodies were increased significantly earlier in PD5-immunized animals compared to those immunized with PD3. The fully protected monkeys showed the earliest HAI Antibody Response. These results underline the usefulness of the anamnestic Antibody Response for supporting protection data. The induction of an early HAI and IgM Antibody Response after challenge suggest a protective role against dengue virus (DENV) infection in monkeys, supporting their use as correlates of protection in vaccine studies. © 2008 Springer-Verlag

Danuta J Herzyk - One of the best experts on this subject based on the ideXlab platform.

  • the t dependent Antibody Response to keyhole limpet hemocyanin in rodents
    Methods of Molecular Biology, 2010
    Co-Authors: Lisa M Plitnick, Danuta J Herzyk
    Abstract:

    Central to the evaluation of potential immunotoxicants is the concept that measurement of multiple parameters is required for the determination of toxicity toward the immune system. A carefully considered integration of endpoints involved in the immune Response should be used to determine an immunotoxic effect. A functional evaluation, specifically the rodent T-cell-dependent Antibody Response (TDAR) model developed for regulated immunotoxicity evaluations, has been established to detect potential immunotoxicity, especially immunosuppression, caused by chemicals and novel pharmaceuticals in development. This chapter provides an overview and detailed procedures involved in the TDAR assay that measures the immune Response (i.e., Antibody production) to an introduced antigen (i.e., keyhole limpet hemocyanin (KLH)) in rats or mice treated with a chemical (e.g., a known immunotoxicant and/or a new drug candidate). The TDAR model of competent immune function requires the participation of multiple effector cells such as antigen presenting cells, T lymphocytes, and B lymphocytes to produce the final product, the antigen-specific Antibody Response. Thus, alterations in the level of Antibody production to the specific antigen may reflect effects on any or all of the cell populations involved in TDAR.

  • primary Antibody Response to keyhole limpet hemocyanin in rat as a model for immunotoxicity evaluation
    Toxicology, 2004
    Co-Authors: Elizabeth R Gore, Jill Gower, Edit Kurali, Juilan Sui, Jane Bynum, Daniela Ennulat, Danuta J Herzyk
    Abstract:

    To address current regulatory expectations on immunotoxicity testing of new chemicals, we describe an animal model that measures the primary Antibody Response to the T-cell dependent antigen, keyhole limpet hemocyanin (KLH). Single immunization with KLH by either footpad (300microg/rat) or intravenous (300microg/kg) route in Sprague Dawley rats resulted in increased germinal center formation in the spleen and a robust anti-KLH IgM (70-388microg/ml) and IgG (230-470microg/ml) Antibody Response with peak detection on Days 5 and 14 post-immunization, respectively. Subcutaneous immunization with KLH (300microg/kg) resulted in a much weaker anti-KLH IgM and IgG ( or =90%) Antibody Responses in the absence of overt toxicity based on evaluation of the standard toxicology parameters. In conclusion, measurement of a rat primary Antibody Response to KLH by ELISA is a reliable and readily standardized method for assessing immunotoxicity of pharmaceuticals.

Carlos López - One of the best experts on this subject based on the ideXlab platform.

  • Anamnestic Antibody Response after viral challenge in monkeys immunized with dengue 2 recombinant fusion proteins
    Archives of Virology, 2008
    Co-Authors: Lidice Bernardo, Jorge Martin, Lisset Hermida, Mayling Alvarez, Irina Prado, Carlos López, Rafael Martínez, Rosmari Rodríguez-roche, Aida Zulueta, Laura Lazo
    Abstract:

    The suitability of dengue 2 envelope domain III recombinant fusion proteins [(fusion (PD5) and insertion (PD3) variants)] for inducing functional antibodies and a protective immune Response in nonhuman primates has been reported. However, the evaluation of the Antibody Response after immunization did not correlate with the protection data as measured by viremia detection. Here, we characterized the anamnestic immune Response after viral challenge in monkeys immunized with the dengue 2 recombinant proteins in an attempt to define correlates of protection useful for vaccine studies. Monkeys immunized with PD5 (most protected group) exhibited an earlier increase in the anti-DENV-2 IgM Response after challenge compared to control animals. Hemagglutination-inhibiting (HAI) antibodies were increased significantly earlier in PD5-immunized animals compared to those immunized with PD3. The fully protected monkeys showed the earliest HAI Antibody Response. These results underline the usefulness of the anamnestic Antibody Response for supporting protection data. The induction of an early HAI and IgM Antibody Response after challenge suggest a protective role against dengue virus (DENV) infection in monkeys, supporting their use as correlates of protection in vaccine studies.

  • Anamnestic Antibody Response after viral challenge in monkeys immunized with dengue 2 recombinant fusion proteins
    'Springer Science and Business Media LLC', 2008
    Co-Authors: Lidice Bernardo, Jorge Martin, Lisset Hermida, Mayling Alvarez, Irina Prado, Carlos López, Rafael Martínez, Rosmari Rodríguez-roche, Aida Zulueta, Laura Lazo
    Abstract:

    The suitability of dengue 2 envelope domain III recombinant fusion proteins [(fusion (PD5) and insertion (PD3) variants)] for inducing functional antibodies and a protective immune Response in nonhuman primates has been reported. However, the evaluation of the Antibody Response after immunization did not correlate with the protection data as measured by viremia detection. Here, we characterized the anamnestic immune Response after viral challenge in monkeys immunized with the dengue 2 recombinant proteins in an attempt to define correlates of protection useful for vaccine studies. Monkeys immunized with PD5 (most protected group) exhibited an earlier increase in the anti-DENV-2 IgM Response after challenge compared to control animals. Hemagglutination- inhibiting (HAI) antibodies were increased significantly earlier in PD5-immunized animals compared to those immunized with PD3. The fully protected monkeys showed the earliest HAI Antibody Response. These results underline the usefulness of the anamnestic Antibody Response for supporting protection data. The induction of an early HAI and IgM Antibody Response after challenge suggest a protective role against dengue virus (DENV) infection in monkeys, supporting their use as correlates of protection in vaccine studies. © 2008 Springer-Verlag

Rafael Martínez - One of the best experts on this subject based on the ideXlab platform.

  • Anamnestic Antibody Response after viral challenge in monkeys immunized with dengue 2 recombinant fusion proteins
    Archives of Virology, 2008
    Co-Authors: Lidice Bernardo, Jorge Martin, Lisset Hermida, Mayling Alvarez, Irina Prado, Carlos López, Rafael Martínez, Rosmari Rodríguez-roche, Aida Zulueta, Laura Lazo
    Abstract:

    The suitability of dengue 2 envelope domain III recombinant fusion proteins [(fusion (PD5) and insertion (PD3) variants)] for inducing functional antibodies and a protective immune Response in nonhuman primates has been reported. However, the evaluation of the Antibody Response after immunization did not correlate with the protection data as measured by viremia detection. Here, we characterized the anamnestic immune Response after viral challenge in monkeys immunized with the dengue 2 recombinant proteins in an attempt to define correlates of protection useful for vaccine studies. Monkeys immunized with PD5 (most protected group) exhibited an earlier increase in the anti-DENV-2 IgM Response after challenge compared to control animals. Hemagglutination-inhibiting (HAI) antibodies were increased significantly earlier in PD5-immunized animals compared to those immunized with PD3. The fully protected monkeys showed the earliest HAI Antibody Response. These results underline the usefulness of the anamnestic Antibody Response for supporting protection data. The induction of an early HAI and IgM Antibody Response after challenge suggest a protective role against dengue virus (DENV) infection in monkeys, supporting their use as correlates of protection in vaccine studies.

  • Anamnestic Antibody Response after viral challenge in monkeys immunized with dengue 2 recombinant fusion proteins
    'Springer Science and Business Media LLC', 2008
    Co-Authors: Lidice Bernardo, Jorge Martin, Lisset Hermida, Mayling Alvarez, Irina Prado, Carlos López, Rafael Martínez, Rosmari Rodríguez-roche, Aida Zulueta, Laura Lazo
    Abstract:

    The suitability of dengue 2 envelope domain III recombinant fusion proteins [(fusion (PD5) and insertion (PD3) variants)] for inducing functional antibodies and a protective immune Response in nonhuman primates has been reported. However, the evaluation of the Antibody Response after immunization did not correlate with the protection data as measured by viremia detection. Here, we characterized the anamnestic immune Response after viral challenge in monkeys immunized with the dengue 2 recombinant proteins in an attempt to define correlates of protection useful for vaccine studies. Monkeys immunized with PD5 (most protected group) exhibited an earlier increase in the anti-DENV-2 IgM Response after challenge compared to control animals. Hemagglutination- inhibiting (HAI) antibodies were increased significantly earlier in PD5-immunized animals compared to those immunized with PD3. The fully protected monkeys showed the earliest HAI Antibody Response. These results underline the usefulness of the anamnestic Antibody Response for supporting protection data. The induction of an early HAI and IgM Antibody Response after challenge suggest a protective role against dengue virus (DENV) infection in monkeys, supporting their use as correlates of protection in vaccine studies. © 2008 Springer-Verlag