Anticarsia gemmatalis

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Flavio Moscardi - One of the best experts on this subject based on the ideXlab platform.

  • Biological and molecular characterization of two Anticarsia gemmatalis multiple nucleopolyhedrovirus clones exhibiting contrasting virulence.
    Journal of Invertebrate Pathology, 2019
    Co-Authors: B. C. Ferreira, Flavio Moscardi, Fernando L Melo, Ana Maria Rodrigues Da Silva, Márcio Martinello Sanches, B. M. Ribeiro, Marlinda Lobo De Souza
    Abstract:

    Abstract Baculovirus natural populations are known to be genetically heterogeneous and such genotypic diversity could have implications in the performance of biocontrol agents. The Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV) has been widely used to control the velvetbean caterpillar, Anticarsia gemmatalis, in Brazil. In the present work, morphological and molecular analyses as well as the biological activity of AgMNPV genotypes derived from a Brazilian field isolate (AgMNPV-79) were carried out. The existence of genotypic variants in the population was confirmed by DNA restriction analysis. Although difference in virulence was observed among the variants, the most (Ag79-01) and the least (AgL-16) virulent clones do not show any morphological and cytopathological changes when compared to the most studied isolate (AgMNPV-2D). The complete genome analysis of the two viral clones showed the presence of single open reading frames (ORFs) of the pe-38 and he65 genes, which contrasts with the two split ORFs present in the genome of the AgMNPV-2D isolate. The viral clone AgL-16 has many variations in the ie-2 and pe-38 genes, which are transcription regulatory genes responsible for the regulation of viral early gene expression during insect cell infection. Furthermore, other genes showed alterations like the odv-e56, which have an essential role in the maturation and envelopment of the ODVs, and bro-a and bro-b genes which were fused to form a single ORF. For the Ag79-01, although the total number of single nucleotide variants (SNVs) was more prominent in the pe-38 gene, its genome showed very few modifications in comparison to the AgMNPV-2D genome.

  • high genetic stability of peroral infection factors from Anticarsia gemmatalis mnpv over 20years of sampling
    Journal of Invertebrate Pathology, 2014
    Co-Authors: Marlinda Lobo De Souza, Flavio Moscardi, Briana C Ferreira, Fernando L Melo, Bergmann Morais Ribeiro
    Abstract:

    Abstract The Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV) has been used as a biopesticide since the early 1980s in Brazil to control the major pest of soybean crops, the velvetbean caterpillar, Anticarsia gemmatalis. To monitor the genetic diversity over space and time we sequenced four pif genes (pif1, pif2, pif3 and pif4) from AgMNPV isolates collected from different regions of South America, as well as of seasonal isolates, sampled during a two-decade field experiment. Although all genes presented low levels of polymorphism, the pif-2 carries a slightly higher number of polymorphic sites. Overall, this study reveals that pif genes have remained stable after 20 years of repeated field application.

  • hemocyte quantitative changes in Anticarsia gemmatalis lepidoptera noctuidae larvae infected by agmnpv
    Brazilian Archives of Biology and Technology, 2010
    Co-Authors: Fabio Andrade, Flavio Moscardi, Maria Claudia Cordeiro De Negreiro, Sheila Michele Levy, Ines Cristina De Batista Fonseca, ângela Maria Ferreira Falleiros
    Abstract:

    The initial effects of the infection by AgMNPV in the total and differential counts of the hemocytes in Anticarsia gemmatalis (Lepidoptera: Noctuidae) larvae were studied. The total number of the hemocytes did not decrease in infected larvae, as it occurred in non infected larvae. In infected larvae, the hemocyte types showed the following frequencies: plasmatocytes - 47.8%, esferulocytes - 25.9%, granulocytes - 15.8%, oenocytoids - 7.2%, prohemocytes - 2.8%, vermicytes - 0,5%. Only the percentage of the granulocytes was different among infected and non infected larvae, indicating that these cells responded quickly to the initial viral infection. These results showed the effective role of the hemocytes in the response of the A. gemmatalis to the infection by AgMNPV. The comprehension of the immunological mechanisms of this insect is an important tool to understand its biological control.

  • susceptibility resistance of Anticarsia gemmatalis larvae to its nucleopolyhedrovirus agmnpv structural study of the peritrophic membrane
    Journal of Invertebrate Pathology, 2007
    Co-Authors: Sheila Michele Levy, Flavio Moscardi, ângela Maria Ferreira Falleiros, Elisa Aparecida Gregorio
    Abstract:

    This investigation compares the peritrophic membrane (PM) morphology along the midgut of susceptible (SL) and resistant (RL) Anticarsia gemmatalis larvae to the AgMNPV. The PM increased the thickness from the anterior to the posterior midgut region in both insects strain; however, the intensity of FITC-WGA reaction of the PM in the RL were greater than in SL. The PM in RL was ultrastructurally constituted by several layers of fibrous/vesicular materials in comparison with the few ones in SL. Our results showed that the structure of PM in the RL could be one of the resistance barriers to AgMNPV.

  • Susceptibility/resistance of Anticarsia gemmatalis larvae to its nucleopolyhedrovirus (AgMNPV): Structural study of the peritrophic membrane
    Journal of Invertebrate Pathology, 2007
    Co-Authors: Sheila Michele Levy, Flavio Moscardi, ângela Maria Ferreira Falleiros, Elisa Aparecida Gregorio
    Abstract:

    This investigation compares the peritrophic membrane (PM) morphology along the midgut of susceptible (SL) and resistant (RL) Anticarsia gemmatalis larvae to the AgMNPV. The PM increased the thickness from the anterior to the posterior midgut region in both insects strain; however, the intensity of FITC-WGA reaction of the PM in the RL were greater than in SL. The PM in RL was ultrastructurally constituted by several layers of fibrous/vesicular materials in comparison with the few ones in SL. Our results showed that the structure of PM in the RL could be one of the resistance barriers to AgMNPV.

Bergmann Morais Ribeiro - One of the best experts on this subject based on the ideXlab platform.

  • Production of viral progeny in insect cells undergoing apoptosis induced by a mutant Anticarsia gemmatalis nucleopolyhedrovirus.
    Microbiological Research, 2020
    Co-Authors: M E B Castro, Bergmann Morais Ribeiro
    Abstract:

    The Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV) is the most successful viral biopesticide in use worldwide. We have demonstrated that despite widespread apoptosis and no protein synthesis at 48 h p.i., UFL-AG-286 cells infected with a mutant of AgMNPV (vApAg), produced significant amounts of budded virus (BVs) and viral DNA late in infection. However, a different susceptible cell line (BTI-Tn5B1-4) showed no signs of apoptosis and produced 3.5 times more budded virus when infected with vApAg. A comparison of DNA from AgMNPV and vApAg digested with the same restriction enzymes showed differences in the restriction pattern, indicating that the vApAg phenotype might be due to a mutation in a gene or genes responsible for directly or indirectly inhibiting apoptosis in UFL-AG-286 cells.

  • high genetic stability of peroral infection factors from Anticarsia gemmatalis mnpv over 20years of sampling
    Journal of Invertebrate Pathology, 2014
    Co-Authors: Marlinda Lobo De Souza, Flavio Moscardi, Briana C Ferreira, Fernando L Melo, Bergmann Morais Ribeiro
    Abstract:

    Abstract The Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV) has been used as a biopesticide since the early 1980s in Brazil to control the major pest of soybean crops, the velvetbean caterpillar, Anticarsia gemmatalis. To monitor the genetic diversity over space and time we sequenced four pif genes (pif1, pif2, pif3 and pif4) from AgMNPV isolates collected from different regions of South America, as well as of seasonal isolates, sampled during a two-decade field experiment. Although all genes presented low levels of polymorphism, the pif-2 carries a slightly higher number of polymorphic sites. Overall, this study reveals that pif genes have remained stable after 20 years of repeated field application.

  • molecular analysis of a mutant Anticarsia gemmatalis multiple nucleopolyhedrovirus agmnpv shows an interruption of an inhibitor of apoptosis gene iap 3 by a new class ii piggybac related insect transposon
    Insect Molecular Biology, 2009
    Co-Authors: M P Carpes, Paolo Marinho De Andrade Zanotto, M E B Castro, J F Nunes, T L Sampaio, Bergmann Morais Ribeiro
    Abstract:

    : A new piggyBac-related transposable element (TE) was found in the genome of a mutant Anticarsia gemmatalis multiple nucleopolyhedrovirus interrupting an inhibitor of apoptosis gene. This mutant virus induces apoptosis upon infection of an Anticarsia gemmatalis cell line, but not in a Trichoplusia ni cell line. The sequence of the new TE (which was named IDT for iap disruptor transposon) has 2531 bp with two DNA sequences flanking a putative Transposase (Tpase) ORF of 1719 bp coding for a protein with 572 amino acids. These structural features are similar to the piggyBac TE, also reported for the first time in the genome of a baculovirus. We have also isolated variants of this new TE from different lepidopteran insect cells and compared their Tpase sequences.

  • Molecular analysis of a mutant Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV) shows an interruption of an inhibitor of apoptosis gene (iap‐3) by a new class‐II piggyBac‐related insect transposon
    Insect Molecular Biology, 2009
    Co-Authors: M P Carpes, Paolo Marinho De Andrade Zanotto, M E B Castro, J F Nunes, T L Sampaio, Bergmann Morais Ribeiro
    Abstract:

    : A new piggyBac-related transposable element (TE) was found in the genome of a mutant Anticarsia gemmatalis multiple nucleopolyhedrovirus interrupting an inhibitor of apoptosis gene. This mutant virus induces apoptosis upon infection of an Anticarsia gemmatalis cell line, but not in a Trichoplusia ni cell line. The sequence of the new TE (which was named IDT for iap disruptor transposon) has 2531 bp with two DNA sequences flanking a putative Transposase (Tpase) ORF of 1719 bp coding for a protein with 572 amino acids. These structural features are similar to the piggyBac TE, also reported for the first time in the genome of a baculovirus. We have also isolated variants of this new TE from different lepidopteran insect cells and compared their Tpase sequences.

  • an Anticarsia gemmatalis multiple nucleopolyhedrovirus mutant vapag induces hemocytes apoptosis in vivo and displays reduced infectivity in larvae of Anticarsia gemmatalis hubner lepidoptera noctuidae
    Virus Research, 2007
    Co-Authors: Eni Braga Da Silveira, Bergmann Morais Ribeiro, Bruno Arrivabene Cordeiro, M E B Castro, Elisa Filgueiras Soares
    Abstract:

    Abstract An Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV) mutant, vApAg, induces apoptosis in a cell culture derived from Anticarsia gemmatalis (UFL-AG-286), reducing viral progeny. We have investigated apoptosis induction in vivo by vApAg in A. gemmatalis larvae and its correlation to infectivity reduction. LC 50 , LD 50 , LT 50 and the mean time to death of larvae were determined for vApAg and AgMNPV. Apoptosis was accessed for hemocytes of infected larvae using light and transmission electron microscopy. All types of hemocytes can be infected by vApAg. After 12 h post-infection (h p.i.), typical cellular modifications associated to nucleopolyhedrovirus infection were observed. Apoptosis becomes evident after 24 h p.i., and massive after 72 h p.i. Necrosis of infected cells was also observed. Despite cell death, hemocytes produced budded viruses and polyhedra. Pl and gh1-type hemocytes presented phagocytic activity. Agarose gel electrophoresis revealed fragmentation of hemocytes DNA at late times post-infection. The LC 50 and LD 50 were between five- and six-fold higher for vApAg. The LT 50 and the mean time to death were higher for vApAg in a same treatment or for a similar mortality induced by AgMNPV. These results show correlation of apoptosis and the reduced infectivity of vApAg in A. gemmatalis larvae.

James E Maruniak - One of the best experts on this subject based on the ideXlab platform.

  • methods for detection of Anticarsia gemmatalis nucleopolyhedrovirus dna in soil
    Applied and Environmental Microbiology, 1999
    Co-Authors: R R De Moraes, James E Maruniak, J E Funderburk
    Abstract:

    Two methods, phenol-ether and magnetic capture-hybridization (MCH), were developed and compared with regard to their sensitivities and abilities to extract the DNA of the insect baculovirus Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV) from soil and to produce DNA amplifiable by PCR. Laboratory experiments were performed with 0.25 g of autoclaved soil inoculated with different viral concentrations to optimize both methods of baculovirus DNA extraction and to determine their sensitivities. Both procedures produced amplifiable DNA; however, the MCH method was 100-fold more sensitive than the phenol-ether procedure. The removal of PCR inhibitors from the soil appeared to be complete when MCH was used as the viral DNA isolation method, because undiluted aliquots of the DNA preparations could be amplified by PCR. The phenol-ether procedure probably did not completely remove PCR inhibitors from the soil, since PCR products were observed only when the AgMNPV DNA preparations were diluted 10- or 100-fold. AgMNPV DNA was detected in field-collected soil samples from 15 to 180 days after virus application when the MCH procedure to isolate DNA was coupled with PCR amplification of the polyhedrin region.

  • physical maps and virulence of Anticarsia gemmatalis nucleopolyhedrovirus genomic variants
    Archives of Virology, 1999
    Co-Authors: James E Maruniak, Alejandra Garciamaruniak, Marlinda Lobo De Souza, Paolo Marinho De Andrade Zanotto, Flavio Moscardi
    Abstract:

    Seventeen plaque purified isolates of two viral preparations of Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV), were analyzed in terms of the genomic changes after digestion of their DNAs with HindIII and PstI restriction enzymes. The 1979 AgMNPV wild type preparation (AgMNPV-’79) resulted in six different variants and the 1985 viral commercial preparation (AgMNPV-’85), in eleven. The genomic variation of all the isolates was mapped showing that those from 1985 presented more heterogeneity with changes mapped in additional sites in comparison to the AgMNPV-’79 variants. Their virulence was compared by infecting two Lepidopteran cell lines, Spodoptera frugiperda (IPLB-SF-21AE) and Anticarsia gemmatalis (UFL-AG-286). The results indicated that there was some difference in virulence within the AgMNPV-’85 variants. This commercial preparation had been applied in soybean fields in Brazil over several years to control the velvetbean caterpillar defoliation.

  • a variable region of Anticarsia gemmatalis nuclear polyhedrosis virus contains tandemly repeated dna sequences
    Virus Research, 1996
    Co-Authors: Alejandra Garciamaruniak, Octavio Henrique O Pavan, James E Maruniak
    Abstract:

    Abstract A variable region (PstI-T fragment) of two genotypic variants of the Anticarsia gemmatalis multiple nucleocapsid nuclear polyhedrosis virus (AgMNPV) was sequenced and compared. This region, which is known to have deletions and duplications in AgMNPV variants, was shown to be formed of units of a 127 bp tandemly repeated sequence containing two 30 bp imperfect palindromes. Southern blot experiments showed that the PstI-T fragment contained one of the four homologous regions mapped interspersed in the AgMNPV genome. The comparison of the nucleotide sequences of the two variants, AgMNPV-2D and AgMNPV-D7, showed that the difference between these two variants in one of these regions (hr4) was caused by the addition or elimination of 381 bp corresponding exactly to three of the 127 bp repeated sequences. An analysis of the sequence showed homology to the homologous regions (hr) of other baculoviruses. The sequence upstream of the repetitive region contained a sequence homologous to the N-terminal portion of the Autographa californica MNPV and Choristoneura fumiferana MNPV p74 gene.

Marlinda Lobo De Souza - One of the best experts on this subject based on the ideXlab platform.

  • Biological and molecular characterization of two Anticarsia gemmatalis multiple nucleopolyhedrovirus clones exhibiting contrasting virulence.
    Journal of Invertebrate Pathology, 2019
    Co-Authors: B. C. Ferreira, Flavio Moscardi, Fernando L Melo, Ana Maria Rodrigues Da Silva, Márcio Martinello Sanches, B. M. Ribeiro, Marlinda Lobo De Souza
    Abstract:

    Abstract Baculovirus natural populations are known to be genetically heterogeneous and such genotypic diversity could have implications in the performance of biocontrol agents. The Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV) has been widely used to control the velvetbean caterpillar, Anticarsia gemmatalis, in Brazil. In the present work, morphological and molecular analyses as well as the biological activity of AgMNPV genotypes derived from a Brazilian field isolate (AgMNPV-79) were carried out. The existence of genotypic variants in the population was confirmed by DNA restriction analysis. Although difference in virulence was observed among the variants, the most (Ag79-01) and the least (AgL-16) virulent clones do not show any morphological and cytopathological changes when compared to the most studied isolate (AgMNPV-2D). The complete genome analysis of the two viral clones showed the presence of single open reading frames (ORFs) of the pe-38 and he65 genes, which contrasts with the two split ORFs present in the genome of the AgMNPV-2D isolate. The viral clone AgL-16 has many variations in the ie-2 and pe-38 genes, which are transcription regulatory genes responsible for the regulation of viral early gene expression during insect cell infection. Furthermore, other genes showed alterations like the odv-e56, which have an essential role in the maturation and envelopment of the ODVs, and bro-a and bro-b genes which were fused to form a single ORF. For the Ag79-01, although the total number of single nucleotide variants (SNVs) was more prominent in the pe-38 gene, its genome showed very few modifications in comparison to the AgMNPV-2D genome.

  • high genetic stability of peroral infection factors from Anticarsia gemmatalis mnpv over 20years of sampling
    Journal of Invertebrate Pathology, 2014
    Co-Authors: Marlinda Lobo De Souza, Flavio Moscardi, Briana C Ferreira, Fernando L Melo, Bergmann Morais Ribeiro
    Abstract:

    Abstract The Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV) has been used as a biopesticide since the early 1980s in Brazil to control the major pest of soybean crops, the velvetbean caterpillar, Anticarsia gemmatalis. To monitor the genetic diversity over space and time we sequenced four pif genes (pif1, pif2, pif3 and pif4) from AgMNPV isolates collected from different regions of South America, as well as of seasonal isolates, sampled during a two-decade field experiment. Although all genes presented low levels of polymorphism, the pif-2 carries a slightly higher number of polymorphic sites. Overall, this study reveals that pif genes have remained stable after 20 years of repeated field application.

  • accumulation of few polyhedra mutants upon serial passage of Anticarsia gemmatalis multiple nucleopolyhedrovirus in cell culture
    Journal of Invertebrate Pathology, 2009
    Co-Authors: Syomara Hakiko Matusita Soares De Rezende, M E B Castro, Marlinda Lobo De Souza
    Abstract:

    Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV) has been widely used to control the velvetbean caterpillar, Anticarsia gemmatalis, in Brazil. To date, AgMNPV has been produced by larval infection and, due to in vivo production limitations and the continuing high demand for the biopesticide, attempts should be made to develop in vitro production of this virus. In order to investigate the effects caused by serial passage of AgMNPV in cell culture, we carried out a total of ten passages and analyzed the morphological and the genomic changes of the virus. After six passages, the many-polyhedra (MP) phenotype started to switch to the few-polyhedra (FP) phenotype which rapidly accumulated in the virus population. Ultrastructural analysis showed typical signs of FP mutant formation such as decrease in the number of polyhedra per cell, polyhedra aberrant morphology and low numbers of virions occluded in the protein matrix. Also enhanced BV production was observed from the fifth passage indicating that FP mutants were becoming predominant in comparison to the wild type virus. Restriction endonuclease analysis of the viral DNA revealed that lower and higher passages had similar profiles indicating that there were no large insertions or deletions or rearrangements in their genomes and indicating the generation of FP mutants instead of defective interfering viruses.

  • characterization of the ecdysteroid udp glucosyltransferase egt gene of Anticarsia gemmatalis nucleopolyhedrovirus
    Virus Genes, 2001
    Co-Authors: Julio C M Rodrigues, Marlinda Lobo De Souza, Fernando Barcellos Razuck, Berghem Ribeiro, David R Oreilly, Lucas Malard Velloso, Francisco Jose Rivera Pinedo, Bergmann Morais Ribeiro
    Abstract:

    The Anticarsia gemmatalis nucelopolyhedrovirus (AgMNPV) egt gene was cloned, sequenced and its expression characterized by RT-PCR and western blot analysis. Sequence analysis of the gene indicated the presence of an open reading frame (ORF) of 1482 nucleotides, which codes for a polypeptide of 494 amino acids. A TATA box and a conserved regulatory sequence (CATT) found in other baculovirus early genes were present in the promoter region of the egt gene. A poly-A consensus sequence was present in the 3′ untranslated region (3′-UTR) of the gene. Homology comparisons showed that the EGT protein of AgMNPV is most closely related (95.9% amino acid sequence identity) to the EGT from the Choristoneura fumiferana DEF nucleopolyhedrovirus (CfDEF). Transcriptional analysis of the AgMNPV egt gene showed that egt-specific transcripts can be detected both early and late in infection. The EGT protein was detected, by western blot analysis, in the intra- (from 12 to 48 h post-infection) and extra-cellular (from 12 to 96 h post-infection) fractions of infected insect cells. The AgMNPV Bgl II-F fragment, which has homology to the AcMNPV ie-1 gene, was cloned and used to cotransfect SF21 cells with the cloned AgMNPV egt gene. EGT activity was observed, suggesting that AgMNPV ie-1 can transactivate egt expression.

  • physical maps and virulence of Anticarsia gemmatalis nucleopolyhedrovirus genomic variants
    Archives of Virology, 1999
    Co-Authors: James E Maruniak, Alejandra Garciamaruniak, Marlinda Lobo De Souza, Paolo Marinho De Andrade Zanotto, Flavio Moscardi
    Abstract:

    Seventeen plaque purified isolates of two viral preparations of Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV), were analyzed in terms of the genomic changes after digestion of their DNAs with HindIII and PstI restriction enzymes. The 1979 AgMNPV wild type preparation (AgMNPV-’79) resulted in six different variants and the 1985 viral commercial preparation (AgMNPV-’85), in eleven. The genomic variation of all the isolates was mapped showing that those from 1985 presented more heterogeneity with changes mapped in additional sites in comparison to the AgMNPV-’79 variants. Their virulence was compared by infecting two Lepidopteran cell lines, Spodoptera frugiperda (IPLB-SF-21AE) and Anticarsia gemmatalis (UFL-AG-286). The results indicated that there was some difference in virulence within the AgMNPV-’85 variants. This commercial preparation had been applied in soybean fields in Brazil over several years to control the velvetbean caterpillar defoliation.

Paolo Marinho De Andrade Zanotto - One of the best experts on this subject based on the ideXlab platform.

  • the pangenome of the Anticarsia gemmatalis multiple nucleopolyhedrovirus agmnpv
    Genome Biology and Evolution, 2016
    Co-Authors: Anderson F Brito, Carla Torres Braconi, Manfred Weidmann, Meik Dilcher, Joao M P Alves, Arthur Gruber, Paolo Marinho De Andrade Zanotto
    Abstract:

    : The alphabaculovirus Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV) is the world's most successful viral bioinsecticide. Through the 1980s and 1990s, this virus was extensively used for biological control of populations of Anticarsia gemmatalis (Velvetbean caterpillar) in soybean crops. During this period, genetic studies identified several variable loci in the AgMNPV; however, most of them were not characterized at the sequence level. In this study we report a full genome comparison among 17 wild-type isolates of AgMNPV. We found the pangenome of this virus to contain at least 167 hypothetical genes, 151 of which are shared by all genomes. The gene bro-a that might be involved in host specificity and carrying transporter is absent in some genomes, and new hypothetical genes were observed. Among these genes there is a unique rnf12-like gene, probably implicated in ubiquitination. Events of gene fission and fusion are common, as four genes have been observed as single or split open reading frames. Gains and losses of genomic fragments (from 20 to 900 bp) are observed within tandem repeats, such as in eight direct repeats and four homologous regions. Most AgMNPV genes present low nucleotide diversity, and variable genes are mainly located in a locus known to evolve through homologous recombination. The evolution of AgMNPV is mainly driven by small indels, substitutions, gain and loss of nucleotide stretches or entire coding sequences. These variations may cause relevant phenotypic alterations, which probably affect the infectivity of AgMNPV. This work provides novel information on genomic evolution of the AgMNPV in particular and of baculoviruses in general.

  • molecular analysis of a mutant Anticarsia gemmatalis multiple nucleopolyhedrovirus agmnpv shows an interruption of an inhibitor of apoptosis gene iap 3 by a new class ii piggybac related insect transposon
    Insect Molecular Biology, 2009
    Co-Authors: M P Carpes, Paolo Marinho De Andrade Zanotto, M E B Castro, J F Nunes, T L Sampaio, Bergmann Morais Ribeiro
    Abstract:

    : A new piggyBac-related transposable element (TE) was found in the genome of a mutant Anticarsia gemmatalis multiple nucleopolyhedrovirus interrupting an inhibitor of apoptosis gene. This mutant virus induces apoptosis upon infection of an Anticarsia gemmatalis cell line, but not in a Trichoplusia ni cell line. The sequence of the new TE (which was named IDT for iap disruptor transposon) has 2531 bp with two DNA sequences flanking a putative Transposase (Tpase) ORF of 1719 bp coding for a protein with 572 amino acids. These structural features are similar to the piggyBac TE, also reported for the first time in the genome of a baculovirus. We have also isolated variants of this new TE from different lepidopteran insect cells and compared their Tpase sequences.

  • Molecular analysis of a mutant Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV) shows an interruption of an inhibitor of apoptosis gene (iap‐3) by a new class‐II piggyBac‐related insect transposon
    Insect Molecular Biology, 2009
    Co-Authors: M P Carpes, Paolo Marinho De Andrade Zanotto, M E B Castro, J F Nunes, T L Sampaio, Bergmann Morais Ribeiro
    Abstract:

    : A new piggyBac-related transposable element (TE) was found in the genome of a mutant Anticarsia gemmatalis multiple nucleopolyhedrovirus interrupting an inhibitor of apoptosis gene. This mutant virus induces apoptosis upon infection of an Anticarsia gemmatalis cell line, but not in a Trichoplusia ni cell line. The sequence of the new TE (which was named IDT for iap disruptor transposon) has 2531 bp with two DNA sequences flanking a putative Transposase (Tpase) ORF of 1719 bp coding for a protein with 572 amino acids. These structural features are similar to the piggyBac TE, also reported for the first time in the genome of a baculovirus. We have also isolated variants of this new TE from different lepidopteran insect cells and compared their Tpase sequences.

  • Structural and phylogenetic relationship of ORF 31 from the Anticarsia gemmatalis MNPV to poly (ADP-ribose) polymerases (PARP)
    Virus Genes, 2008
    Co-Authors: Juliana Velasco De Castro Oliveira, Fernando L Melo, Camila Malta Romano, Atila Iamarino, Thais Sampaio Rizzi, Fernanda Peres Yeda, Charlotte Marianna Hársi, José Luiz Caldas Wolff, Paolo Marinho De Andrade Zanotto
    Abstract:

    ORF 31 is a unique baculovirus gene in the genome of Anticarsia gemmatalis multiple nucleopolyhedrovirus isolate 2D (AgMNPV-2D). It encodes a putative polypeptide of 369 aa homologous to poly (ADP-ribose) polymerase (PARP) found in the genomes of several organisms. Moreover, we found a phylogenetic association with Group I PARP proteins and a 3D homology model of its conserved PARP C-terminal catalytic domain indicating that had almost an exact spatial superimposition of

  • structural and phylogenetic relationship of orf 31 from the Anticarsia gemmatalis mnpv to poly adp ribose polymerases parp
    Virus Genes, 2008
    Co-Authors: Juliana Velasco De Castro Oliveira, Fernando L Melo, Camila Malta Romano, Atila Iamarino, Thais Sampaio Rizzi, Fernanda Peres Yeda, Charlotte Marianna Hársi, José Luiz Caldas Wolff, Paolo Marinho De Andrade Zanotto
    Abstract:

    ORF 31 is a unique baculovirus gene in the genome of Anticarsia gemmatalis multiple nucleopolyhedrovirus isolate 2D (AgMNPV-2D). It encodes a putative polypeptide of 369 aa homologous to poly (ADP-ribose) polymerase (PARP) found in the genomes of several organisms. Moreover, we found a phylogenetic association with Group I PARP proteins and a 3D homology model of its conserved PARP C-terminal catalytic domain indicating that had almost an exact spatial superimposition of <1 A with other PARP available structures. The 5′ end of ORF 31 mRNA was located at the first nucleotide of a CATT motif at position −27. Using real-time PCR we detected transcripts at 3 h post-infection (p.i.) increasing until 24 h p.i., which coincides with the onset of DNA replication, suggestive of a possible role in DNA metabolism.