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Teruhiro Takabe - One of the best experts on this subject based on the ideXlab platform.
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Requirement of alkanes for salt tolerance of Cyanobacteria: characterization of alkane synthesis genes from salt-sensitive Synechococcus elongatus PCC7942 and salt-tolerant Aphanothece halophytica.
Letters in applied microbiology, 2018Co-Authors: T. Yamamori, Yoshito Tanaka, Hakuto Kageyama, Teruhiro TakabeAbstract:Cyanobacteria have been attracting great interest in the research area of biofuel production. All Cyanobacteria contain C15 -C19 hydrocarbons, but physiological roles of hydrocarbons remain to be clarified. Recently, two universal but mutually exclusive hydrocarbon production pathways in Cyanobacteria were discovered. In this study, we constructed a deletion mutant of alkane synthesis genes in fresh water cyanobacterium Synechococcus elongates PCC 7942. The mutant was incapable to produce alkanes and exhibited normal growth phenotype at low salinity. But, the mutant became salt sensitive. Overexpression of alkane synthesis genes from halotolerant Aphanothece halophytica in Synechococcus PCC7942 restored the growth defect. The alkane synthesis gene from halotolerant cyanobacterium A. halophytica was salt induced and produced a significant amount of alkanes at high salinity. These results indicate the requirement of alkanes for salt tolerance, and the alkane synthesis genes from A. halophytica could be a promising candidate for future biofuel application. SIGNIFICANCE AND IMPACT OF THE STUDY Cyanobacteria have been attracting great interest in the research area of biofuel production. All Cyanobacteria contain C15 -C19 hydrocarbons, but physiological roles of hydrocarbons remain to be clarified. In this study, it was found that the deletion mutant of alkane synthesis genes in fresh water cyanobacterium Synechococcus elongates PCC 7942 was incapable to produce alkanes and salt sensitive. The alkane synthesis gene from halotolerant cyanobacterium Aphanothece halophytica was salt induced and produced a significant amount of alkanes at high salinity. These results demonstrate the alkane synthesis genes from A. halophytica could be a promising candidate for future biofuel application.
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Identification and upregulation of biosynthetic genes required for accumulation of mycosporine-2-glycine under salt stress conditions in the halotolerant cyanobacterium Aphanothece halophytica
Applied and Environmental Microbiology, 2014Co-Authors: Rungaroon Waditee-sirisattha, Warangkana Sopun, Yoshito Tanaka, Hakuto Kageyama, Teruhiro TakabeAbstract:Mycosporine-like amino acids (MAAs) are valuable molecules that are the basis for important photoprotective constituents. Here we report molecular analysis of mycosporine-like amino acid biosynthetic genes from the halotolerant cyanobacterium Aphanothece halophytica , which can survive at high salinity and alkaline pH. This extremophile was found to have a unique MAA core (4-deoxygadusol)-synthesizing gene separated from three other genes. In vivo analysis showed accumulation of the mycosporine-2-glycine but not shinorine or mycosporine-glycine. Mycosporine-2-glycine accumulation was stimulated more under the stress condition of high salinity than UV-B radiation. The Aphanothece MAA biosynthetic genes also manifested a strong transcript level response to salt stress. Furthermore, the transformed Escherichia coli and Synechococcus strains expressing four putative Aphanothece MAA genes under the control of a native promoter were found to be capable of synthesizing mycosporine-2-glycine. The accumulation level of mycosporine-2-glycine was again higher under the high-salinity condition. In the transformed E. coli cells, its level was approximately 85.2 ± 0.7 μmol/g (dry weight). Successful production of a large amount of mycosporine in these cells provides a new opportunity in the search for an alternative natural sunscreen compound source.
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Physiological, biochemical and molecular responses of the halophilic cyanobacterium Aphanothece halophytica to Pi-deficiency
European Journal of Phycology, 2013Co-Authors: Keshwanand Tripathi, Hakuto Kageyama, Teruhiro Takabe, Naveen K. Sharma, Ashwani K. RaiAbstract:We studied the responses of a halophilic cyanobacterium Aphanothece halophytica at surplus (normal composition of growth medium containing 125 µM PO43−), sufficient (the minimum concentration supporting optimal growth, 22 µM PO43−) and deficient (no external supply of Pi) concentrations of inorganic phosphate (Pi). The cyanobacterium was able to grow well in Pi-deficient conditions until the end of incubation (14 days), though at a marginally reduced rate. The cellular P-quota in Pi-surplus cells at the end of incubation was 2.7 times that of their initial P-quota (0.75 µmol mg protein−1), and remained fairly high (0.442 µmol mg protein−1) even in Pi-deficient medium. However, cultures growing in Pi-sufficient medium (22 µM PO43−), upon transfer to Pi-deficient medium, exhibited a rapid decline in cellular P level. Furthermore, cells growing in Pi-surplus medium showed a rapid efflux of P into the external medium. Aphanothece halophytica exhibited a biphasic phosphate transport system involving both high-...
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halotolerant cyanobacterium Aphanothece halophytica contains an na dependent f1f0 atp synthase with a potential role in salt stress tolerance
Journal of Biological Chemistry, 2011Co-Authors: Hakuto Kageyama, Teruhiro Takabe, Aran Incharoensakdi, Worrawat Promden, Kanteera Soontharapirakkul, Nana Yamada, Atsuko IwamotokiharaAbstract:Aphanothece halophytica is a halotolerant alkaliphilic cyanobacterium that can grow in media of up to 3.0 m NaCl and pH 11. Here, we show that in addition to a typical H+-ATP synthase, Aphanothece halophytica contains a putative F1F0-type Na+-ATP synthase (ApNa+-ATPase) operon (ApNa+-atp). The operon consists of nine genes organized in the order of putative subunits β, ϵ, I, hypothetical protein, a, c, b, α, and γ. Homologous operons could also be found in some cyanobacteria such as Synechococcus sp. PCC 7002 and Acaryochloris marina MBIC11017. The ApNa+-atp operon was isolated from the A. halophytica genome and transferred into an Escherichia coli mutant DK8 (Δatp) deficient in ATP synthase. The inverted membrane vesicles of E. coli DK8 expressing ApNa+-ATPase exhibited Na+-dependent ATP hydrolysis activity, which was inhibited by monensin and tributyltin chloride, but not by the protonophore, carbonyl cyanide m-chlorophenyl hydrazone (CCCP). The Na+ ion protected the inhibition of ApNa+-ATPase by N,N′-dicyclohexylcarbodiimide. The ATP synthesis activity was also observed using the Na+-loaded inverted membrane vesicles. Expression of the ApNa+-atp operon in the heterologous cyanobacterium Synechococcus sp. PCC 7942 showed its localization in the cytoplasmic membrane fractions and increased tolerance to salt stress. These results indicate that A. halophytica has additional Na+-dependent F1F0-ATPase in the cytoplasmic membrane playing a potential role in salt-stress tolerance.
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Halotolerant cyanobacterium Aphanothece halophytica contains an Na+-dependent F1F0-ATP synthase with a potential role in salt-stress tolerance.
The Journal of biological chemistry, 2011Co-Authors: Kanteera Soontharapirakkul, Hakuto Kageyama, Aran Incharoensakdi, Worrawat Promden, Nana Yamada, Atsuko Iwamoto-kihara, Teruhiro TakabeAbstract:Aphanothece halophytica is a halotolerant alkaliphilic cyanobacterium that can grow in media of up to 3.0 m NaCl and pH 11. Here, we show that in addition to a typical H+-ATP synthase, Aphanothece halophytica contains a putative F1F0-type Na+-ATP synthase (ApNa+-ATPase) operon (ApNa+-atp). The operon consists of nine genes organized in the order of putative subunits β, ϵ, I, hypothetical protein, a, c, b, α, and γ. Homologous operons could also be found in some cyanobacteria such as Synechococcus sp. PCC 7002 and Acaryochloris marina MBIC11017. The ApNa+-atp operon was isolated from the A. halophytica genome and transferred into an Escherichia coli mutant DK8 (Δatp) deficient in ATP synthase. The inverted membrane vesicles of E. coli DK8 expressing ApNa+-ATPase exhibited Na+-dependent ATP hydrolysis activity, which was inhibited by monensin and tributyltin chloride, but not by the protonophore, carbonyl cyanide m-chlorophenyl hydrazone (CCCP). The Na+ ion protected the inhibition of ApNa+-ATPase by N,N′-dicyclohexylcarbodiimide. The ATP synthesis activity was also observed using the Na+-loaded inverted membrane vesicles. Expression of the ApNa+-atp operon in the heterologous cyanobacterium Synechococcus sp. PCC 7942 showed its localization in the cytoplasmic membrane fractions and increased tolerance to salt stress. These results indicate that A. halophytica has additional Na+-dependent F1F0-ATPase in the cytoplasmic membrane playing a potential role in salt-stress tolerance.
Aran Incharoensakdi - One of the best experts on this subject based on the ideXlab platform.
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Factors affecting biohydrogen production by unicellular halotolerant cyanobacterium Aphanothece halophytica
Journal of Applied Phycology, 2012Co-Authors: Samart Taikhao, Aran Incharoensakdi, Suwannee Junyapoon, Saranya PhunpruchAbstract:The effects of several physiological parameters on H2 production rate in the unicellular halotolerant cyanobacterium Aphanothece halophytica were investigated. Under nitrogen deprivation, the growth of cells was inhibited, but H2 production rate was enhanced approximately fourfold. Interestingly, cells grown under sulfur deprivation exhibited a decrease in cell growth, H2 production rate, and bidirectional hydrogenase activity. Glucose was the preferred sugar source for H2 production by A. halophytica, but H2 production decreased at high glucose concentrations. H2 production rate was optimum when cells were grown in the presence of 0.75 M NaCl, or 0.4 μM Fe3+, or 1 μM Ni2+. The optimum light intensity and temperature for H2 production were 30 μmol photons m−2 s−1 and 35 °C, respectively. A two-stage culture of A. halophytica was performed in order to overcome the reduction of cell growth in N-free medium. In the first stage, cells were grown in normal medium to accumulate biomass, and in the second stage, H2 production by the obtained biomass was induced by growing cells in N-free medium supplemented with various chemicals for 24 h. A. halophytica grown in N-free medium containing various MgSO4 concentrations had a high H2 production rate between 11.432 and 12.767 μmol H2 mg chlorophyll a (chl a)−1 h−1, a 30-fold increase compared to cells grown in normal medium. The highest rate of 13.804 μmol H2 mg chl a −1 h−1 was obtained when the N-free growth medium contained 0.4 μM Fe3+. These results suggested the possibility of using A. halophytica and some other halotolerant cyanobacteria thriving under extreme environmental conditions in the sea as potential sources for H2 production in the future.
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halotolerant cyanobacterium Aphanothece halophytica contains an na dependent f1f0 atp synthase with a potential role in salt stress tolerance
Journal of Biological Chemistry, 2011Co-Authors: Hakuto Kageyama, Teruhiro Takabe, Aran Incharoensakdi, Worrawat Promden, Kanteera Soontharapirakkul, Nana Yamada, Atsuko IwamotokiharaAbstract:Aphanothece halophytica is a halotolerant alkaliphilic cyanobacterium that can grow in media of up to 3.0 m NaCl and pH 11. Here, we show that in addition to a typical H+-ATP synthase, Aphanothece halophytica contains a putative F1F0-type Na+-ATP synthase (ApNa+-ATPase) operon (ApNa+-atp). The operon consists of nine genes organized in the order of putative subunits β, ϵ, I, hypothetical protein, a, c, b, α, and γ. Homologous operons could also be found in some cyanobacteria such as Synechococcus sp. PCC 7002 and Acaryochloris marina MBIC11017. The ApNa+-atp operon was isolated from the A. halophytica genome and transferred into an Escherichia coli mutant DK8 (Δatp) deficient in ATP synthase. The inverted membrane vesicles of E. coli DK8 expressing ApNa+-ATPase exhibited Na+-dependent ATP hydrolysis activity, which was inhibited by monensin and tributyltin chloride, but not by the protonophore, carbonyl cyanide m-chlorophenyl hydrazone (CCCP). The Na+ ion protected the inhibition of ApNa+-ATPase by N,N′-dicyclohexylcarbodiimide. The ATP synthesis activity was also observed using the Na+-loaded inverted membrane vesicles. Expression of the ApNa+-atp operon in the heterologous cyanobacterium Synechococcus sp. PCC 7942 showed its localization in the cytoplasmic membrane fractions and increased tolerance to salt stress. These results indicate that A. halophytica has additional Na+-dependent F1F0-ATPase in the cytoplasmic membrane playing a potential role in salt-stress tolerance.
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Halotolerant cyanobacterium Aphanothece halophytica contains an Na+-dependent F1F0-ATP synthase with a potential role in salt-stress tolerance.
The Journal of biological chemistry, 2011Co-Authors: Kanteera Soontharapirakkul, Hakuto Kageyama, Aran Incharoensakdi, Worrawat Promden, Nana Yamada, Atsuko Iwamoto-kihara, Teruhiro TakabeAbstract:Aphanothece halophytica is a halotolerant alkaliphilic cyanobacterium that can grow in media of up to 3.0 m NaCl and pH 11. Here, we show that in addition to a typical H+-ATP synthase, Aphanothece halophytica contains a putative F1F0-type Na+-ATP synthase (ApNa+-ATPase) operon (ApNa+-atp). The operon consists of nine genes organized in the order of putative subunits β, ϵ, I, hypothetical protein, a, c, b, α, and γ. Homologous operons could also be found in some cyanobacteria such as Synechococcus sp. PCC 7002 and Acaryochloris marina MBIC11017. The ApNa+-atp operon was isolated from the A. halophytica genome and transferred into an Escherichia coli mutant DK8 (Δatp) deficient in ATP synthase. The inverted membrane vesicles of E. coli DK8 expressing ApNa+-ATPase exhibited Na+-dependent ATP hydrolysis activity, which was inhibited by monensin and tributyltin chloride, but not by the protonophore, carbonyl cyanide m-chlorophenyl hydrazone (CCCP). The Na+ ion protected the inhibition of ApNa+-ATPase by N,N′-dicyclohexylcarbodiimide. The ATP synthesis activity was also observed using the Na+-loaded inverted membrane vesicles. Expression of the ApNa+-atp operon in the heterologous cyanobacterium Synechococcus sp. PCC 7942 showed its localization in the cytoplasmic membrane fractions and increased tolerance to salt stress. These results indicate that A. halophytica has additional Na+-dependent F1F0-ATPase in the cytoplasmic membrane playing a potential role in salt-stress tolerance.
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Na+-stimulated ATPase of alkaliphilic halotolerant cyanobacterium Aphanothece halophytica translocates Na+ into proteoliposomes via Na+ uniport mechanism
BMC biochemistry, 2010Co-Authors: Kanteera Soontharapirakkul, Aran IncharoensakdiAbstract:Background When cells are exposed to high salinity conditions, they develop a mechanism to extrude excess Na+ from cells to maintain the cytoplasmic Na+ concentration. Until now, the ATPase involved in Na+ transport in cyanobacteria has not been characterized. Here, the characterization of ATPase and its role in Na+ transport of alkaliphilic halotolerant Aphanothece halophytica were investigated to understand the survival mechanism of A. halophytica under high salinity conditions.
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na stimulated atpase of alkaliphilic halotolerant cyanobacterium Aphanothece halophytica translocates na into proteoliposomes via na uniport mechanism
BMC Biochemistry, 2010Co-Authors: Kanteera Soontharapirakkul, Aran IncharoensakdiAbstract:Background When cells are exposed to high salinity conditions, they develop a mechanism to extrude excess Na+ from cells to maintain the cytoplasmic Na+ concentration. Until now, the ATPase involved in Na+ transport in cyanobacteria has not been characterized. Here, the characterization of ATPase and its role in Na+ transport of alkaliphilic halotolerant Aphanothece halophytica were investigated to understand the survival mechanism of A. halophytica under high salinity conditions.
Maria Isabel Queiroz - One of the best experts on this subject based on the ideXlab platform.
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produccion de pigmentos por Aphanothece microscopica nageli a partir de residuos industriales lacteos
Ingeniare. Revista chilena de ingeniería, 2017Co-Authors: Nivia Maria Streit, Eduardo Jacoblopes, Leila Queiroz Zepka, Luis G Ramirezmerida, Maria Isabel QueirozAbstract:espanolLos sistemas basados en el uso de microalgas para la obtencion de bioproductos son considerados un area prometedora para aplicaciones industriales. El objetivo del trabajo fue evaluar la produccion de ficobiliproteinas y clorofila-a cuando Aphanothece microscopica Nageli es cultivada en sistemas autotroficos y heterotroficos. Para ello, se desarrollaron sistemas de cultivos heterotroficos (en ausencia de luz) y autotroficos (2 klux de intensidad luminosa) en biorreactores a pH 7,6, 20 °C, 1 vvm de aireacion, empleando como medios de cultivo un efluente de industria lactea con relacion C/N 20 y N/P 10, y BG11. Inoculo inicial 200 mg/L. Se efectuo el monitoreo de concentraciones de clorofila-a y ficobiliproteinas en fase logaritmica y estacionaria. Se observaron las mayores concentraciones de pigmento en la fase logaritmica: mayores concentraciones de clorofila-a en cultivos autotroficos y predominio de aloficocianina y ficoeritrina en cultivos heterotroficos. Los resultados mostraron el efecto de las variables en estudio para la produccion de pigmentos, en la fase de crecimiento logaritmico, lo que indica la posibilidad de producir en sistemas heterotroficos de Aphanothece microscopica Nageli, aproximadamente 3.185 ton/ ano de biomasa, para 3.127 kg/ano clorofila, 232.825 kg/ano ficocianina, 47.775 kg/ano aloficocianina y 7.008 kg/ano ficoeritrina, cuando se utiliza efluente de industria lactea como medio de cultivo. EnglishThe systems that use microalgae to obtain bioproducts are considered a promising area for industrial applications. The objective of this work was to evaluate the production of phycobiliproteins and chlorophyll-a by Aphanothece microscopica Nageli, when it is cultivated in autotrophic and heterotrophic systems. Thus, the cultures were grown in heterotrophic (absence of light) and autotrophic (2 klux of light intensity) system, in bioreactors at pH 7.6, 20 oC, 1 vvm aeration, using a dairy industry effluent, with C/N 20 and N/P 10, and BG11 as culture media. Initial inoculum 200 mg/L. The concentrations of chlorophyll-a-andphycobiliproteins in logarithmic and stationary phase were monitored. The highest concentrations of pigment were observed in logarithmic phase: The highest concentrations of chlorophyll-a in autotrophic cultures, and predominance of allophycocyanin and phycoerythrin in heterotrophic cultures. The results show the effect of the variables under study for pigment production, bearing in mind the logarithmic growth phase, suggesting the possibility of producing in heterotrophic systems by Aphanothece microscopica Nageli, around 3,185 ton/year of biomass, and 3,127 kg/year of chlorophyll, 232,825 kg/year of phycocyanin, 47,775 kg/year of allophycocyanin and 7,008 kg/year of phycoerythrin, when dairy effluent is used as culture medium
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Influence of temperature in phosphate removal by microalgae in heterotrophic bioreactors
Instituto de Pesquisas Ambientais em Bacias Hidrográficas (IPABHi), 2012Co-Authors: Maria Isabel Queiroz, Eduardo Jacob-lopes, Adriana Goncalves Da Silva Manetti, Juliana Guerra VieiraAbstract:The removal of total dissolved phosphate by the cyanobacteria Aphanothece microscopica Nägeli cultivated heterotrophically in dairy processing wastewater was investigated in this work. The experiments were carried out in bioreactors operating in a batch mode, fed with 4.5 L of wastewater. The experimental conditions were as follows: initial cell concentration of 0.2 g/L, pH adjusted to 7.6, isothermal reactor operating at temperatures of 10, 20 and 30ºC with absence of light and continuous aeration of 1 VVM. The results showed that phosphate removal is strongly dependent on process temperature. A. microscopica Nägeli was effective in the removal phosphate achieving removal rates of 3.77 mg/L.h, which reflected in the conversion of 98.4% in hydraulic detention times of 24 h
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Growth of Aphanothece microscopica nägeli on exogenous sugars = Cultivo da Aphanothece microscopica nägeli a partir de açúcares
Bioscience Journal, 2011Co-Authors: Reinaldo Gaspar Bastos, Paola Rizzo De Paiva, Maurício Rigo, Graziela Veiga, Maria Isabel QueirozAbstract:Biological processes for wastewater treatment generally produce a lot of biomass or active sludge without reuse. In this context, incorporation of organic matter and nutrients from agro industrial effluents into cell mass for single-cell protein allowed application of sustainable process. Cyanobacteria could be used due to its versatile metabolism. So, the aim of this paper was evaluate the growth of cyanobacteria Aphanothece microscopica Nageli growth on heterotrophic medium with glucose, lactose and sucrose. Growth curves indicated that cultivation of cyanobaterial on the dark depend the type of carbon source and there are different mechanisms for glucose, fructose and sucrose consumption. Results suggest a useful application of cyanobacteria on organic matter removal from wastewater.
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growth of Aphanothece microscopica nageli on exogenous sugars cultivo da Aphanothece microscopica nageli a partir de acucares
Bioscience Journal, 2011Co-Authors: Reinaldo Gaspar Bastos, Paola Rizzo De Paiva, Maurício Rigo, Graziela Veiga, Maria Isabel QueirozAbstract:Biological processes for wastewater treatment generally produce a lot of biomass or active sludge without reuse. In this context, incorporation of organic matter and nutrients from agro industrial effluents into cell mass for single-cell protein allowed application of sustainable process. Cyanobacteria could be used due to its versatile metabolism. So, the aim of this paper was evaluate the growth of cyanobacteria Aphanothece microscopica Nageli growth on heterotrophic medium with glucose, lactose and sucrose. Growth curves indicated that cultivation of cyanobaterial on the dark depend the type of carbon source and there are different mechanisms for glucose, fructose and sucrose consumption. Results suggest a useful application of cyanobacteria on organic matter removal from wastewater.
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cod removal of parboilized rice wastewater by cyanobacteria Aphanothece microscopica nageli remocao de dqo do efluente da parboilizacao do arroz pela cianobacteria Aphanothece microscopica nageli
Revista Brasileira de Engenharia de Biossistemas, 2011Co-Authors: Reinaldo Gaspar Bastos, Maria Isabel Queiroz, L. Q. Zepka, G. Volpato, M. S. Garcia, E JacoblopesAbstract:Cyanobacteria are photosynthetic prokaryotes organisms used on single-cell protein production and wastewater treatment for nitrogen and phosphorus removal. Moreover, some strains have the particular characteristic of growing in the dark on simple molecules such as acetate, glucose and organic acids, and consequently reduction of Chemical Oxygen Demand (COD) from nutrient medium. Aphanothece microscopica Nageli is a cyanobacteria frequently found in south of Brazil, which has been studied with respect to residues valorization in the single-cell protein production. Wastewater from rice parboilization process presents characteristics that suggest the removal of nutrients and organic matter by incorporation into a biomass. The aim of this paper is to extend the findings of the previous studies about heterotrophic metabolism application of these cyanobacteria on wastewater treatment, considering influence of temperature and inoculum amount. Experiments indicated that Aphanothece microscopica Nageli presented high potential of organic matter removal from parboilized rice effluent by 300 mgL-1 inoculum at temperature range 25-35oC.
Kanteera Soontharapirakkul - One of the best experts on this subject based on the ideXlab platform.
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halotolerant cyanobacterium Aphanothece halophytica contains an na dependent f1f0 atp synthase with a potential role in salt stress tolerance
Journal of Biological Chemistry, 2011Co-Authors: Hakuto Kageyama, Teruhiro Takabe, Aran Incharoensakdi, Worrawat Promden, Kanteera Soontharapirakkul, Nana Yamada, Atsuko IwamotokiharaAbstract:Aphanothece halophytica is a halotolerant alkaliphilic cyanobacterium that can grow in media of up to 3.0 m NaCl and pH 11. Here, we show that in addition to a typical H+-ATP synthase, Aphanothece halophytica contains a putative F1F0-type Na+-ATP synthase (ApNa+-ATPase) operon (ApNa+-atp). The operon consists of nine genes organized in the order of putative subunits β, ϵ, I, hypothetical protein, a, c, b, α, and γ. Homologous operons could also be found in some cyanobacteria such as Synechococcus sp. PCC 7002 and Acaryochloris marina MBIC11017. The ApNa+-atp operon was isolated from the A. halophytica genome and transferred into an Escherichia coli mutant DK8 (Δatp) deficient in ATP synthase. The inverted membrane vesicles of E. coli DK8 expressing ApNa+-ATPase exhibited Na+-dependent ATP hydrolysis activity, which was inhibited by monensin and tributyltin chloride, but not by the protonophore, carbonyl cyanide m-chlorophenyl hydrazone (CCCP). The Na+ ion protected the inhibition of ApNa+-ATPase by N,N′-dicyclohexylcarbodiimide. The ATP synthesis activity was also observed using the Na+-loaded inverted membrane vesicles. Expression of the ApNa+-atp operon in the heterologous cyanobacterium Synechococcus sp. PCC 7942 showed its localization in the cytoplasmic membrane fractions and increased tolerance to salt stress. These results indicate that A. halophytica has additional Na+-dependent F1F0-ATPase in the cytoplasmic membrane playing a potential role in salt-stress tolerance.
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Halotolerant cyanobacterium Aphanothece halophytica contains an Na+-dependent F1F0-ATP synthase with a potential role in salt-stress tolerance.
The Journal of biological chemistry, 2011Co-Authors: Kanteera Soontharapirakkul, Hakuto Kageyama, Aran Incharoensakdi, Worrawat Promden, Nana Yamada, Atsuko Iwamoto-kihara, Teruhiro TakabeAbstract:Aphanothece halophytica is a halotolerant alkaliphilic cyanobacterium that can grow in media of up to 3.0 m NaCl and pH 11. Here, we show that in addition to a typical H+-ATP synthase, Aphanothece halophytica contains a putative F1F0-type Na+-ATP synthase (ApNa+-ATPase) operon (ApNa+-atp). The operon consists of nine genes organized in the order of putative subunits β, ϵ, I, hypothetical protein, a, c, b, α, and γ. Homologous operons could also be found in some cyanobacteria such as Synechococcus sp. PCC 7002 and Acaryochloris marina MBIC11017. The ApNa+-atp operon was isolated from the A. halophytica genome and transferred into an Escherichia coli mutant DK8 (Δatp) deficient in ATP synthase. The inverted membrane vesicles of E. coli DK8 expressing ApNa+-ATPase exhibited Na+-dependent ATP hydrolysis activity, which was inhibited by monensin and tributyltin chloride, but not by the protonophore, carbonyl cyanide m-chlorophenyl hydrazone (CCCP). The Na+ ion protected the inhibition of ApNa+-ATPase by N,N′-dicyclohexylcarbodiimide. The ATP synthesis activity was also observed using the Na+-loaded inverted membrane vesicles. Expression of the ApNa+-atp operon in the heterologous cyanobacterium Synechococcus sp. PCC 7942 showed its localization in the cytoplasmic membrane fractions and increased tolerance to salt stress. These results indicate that A. halophytica has additional Na+-dependent F1F0-ATPase in the cytoplasmic membrane playing a potential role in salt-stress tolerance.
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Na+-stimulated ATPase of alkaliphilic halotolerant cyanobacterium Aphanothece halophytica translocates Na+ into proteoliposomes via Na+ uniport mechanism
BMC biochemistry, 2010Co-Authors: Kanteera Soontharapirakkul, Aran IncharoensakdiAbstract:Background When cells are exposed to high salinity conditions, they develop a mechanism to extrude excess Na+ from cells to maintain the cytoplasmic Na+ concentration. Until now, the ATPase involved in Na+ transport in cyanobacteria has not been characterized. Here, the characterization of ATPase and its role in Na+ transport of alkaliphilic halotolerant Aphanothece halophytica were investigated to understand the survival mechanism of A. halophytica under high salinity conditions.
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na stimulated atpase of alkaliphilic halotolerant cyanobacterium Aphanothece halophytica translocates na into proteoliposomes via na uniport mechanism
BMC Biochemistry, 2010Co-Authors: Kanteera Soontharapirakkul, Aran IncharoensakdiAbstract:Background When cells are exposed to high salinity conditions, they develop a mechanism to extrude excess Na+ from cells to maintain the cytoplasmic Na+ concentration. Until now, the ATPase involved in Na+ transport in cyanobacteria has not been characterized. Here, the characterization of ATPase and its role in Na+ transport of alkaliphilic halotolerant Aphanothece halophytica were investigated to understand the survival mechanism of A. halophytica under high salinity conditions.
Hakuto Kageyama - One of the best experts on this subject based on the ideXlab platform.
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Requirement of alkanes for salt tolerance of Cyanobacteria: characterization of alkane synthesis genes from salt-sensitive Synechococcus elongatus PCC7942 and salt-tolerant Aphanothece halophytica.
Letters in applied microbiology, 2018Co-Authors: T. Yamamori, Yoshito Tanaka, Hakuto Kageyama, Teruhiro TakabeAbstract:Cyanobacteria have been attracting great interest in the research area of biofuel production. All Cyanobacteria contain C15 -C19 hydrocarbons, but physiological roles of hydrocarbons remain to be clarified. Recently, two universal but mutually exclusive hydrocarbon production pathways in Cyanobacteria were discovered. In this study, we constructed a deletion mutant of alkane synthesis genes in fresh water cyanobacterium Synechococcus elongates PCC 7942. The mutant was incapable to produce alkanes and exhibited normal growth phenotype at low salinity. But, the mutant became salt sensitive. Overexpression of alkane synthesis genes from halotolerant Aphanothece halophytica in Synechococcus PCC7942 restored the growth defect. The alkane synthesis gene from halotolerant cyanobacterium A. halophytica was salt induced and produced a significant amount of alkanes at high salinity. These results indicate the requirement of alkanes for salt tolerance, and the alkane synthesis genes from A. halophytica could be a promising candidate for future biofuel application. SIGNIFICANCE AND IMPACT OF THE STUDY Cyanobacteria have been attracting great interest in the research area of biofuel production. All Cyanobacteria contain C15 -C19 hydrocarbons, but physiological roles of hydrocarbons remain to be clarified. In this study, it was found that the deletion mutant of alkane synthesis genes in fresh water cyanobacterium Synechococcus elongates PCC 7942 was incapable to produce alkanes and salt sensitive. The alkane synthesis gene from halotolerant cyanobacterium Aphanothece halophytica was salt induced and produced a significant amount of alkanes at high salinity. These results demonstrate the alkane synthesis genes from A. halophytica could be a promising candidate for future biofuel application.
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Identification and upregulation of biosynthetic genes required for accumulation of mycosporine-2-glycine under salt stress conditions in the halotolerant cyanobacterium Aphanothece halophytica
Applied and Environmental Microbiology, 2014Co-Authors: Rungaroon Waditee-sirisattha, Warangkana Sopun, Yoshito Tanaka, Hakuto Kageyama, Teruhiro TakabeAbstract:Mycosporine-like amino acids (MAAs) are valuable molecules that are the basis for important photoprotective constituents. Here we report molecular analysis of mycosporine-like amino acid biosynthetic genes from the halotolerant cyanobacterium Aphanothece halophytica , which can survive at high salinity and alkaline pH. This extremophile was found to have a unique MAA core (4-deoxygadusol)-synthesizing gene separated from three other genes. In vivo analysis showed accumulation of the mycosporine-2-glycine but not shinorine or mycosporine-glycine. Mycosporine-2-glycine accumulation was stimulated more under the stress condition of high salinity than UV-B radiation. The Aphanothece MAA biosynthetic genes also manifested a strong transcript level response to salt stress. Furthermore, the transformed Escherichia coli and Synechococcus strains expressing four putative Aphanothece MAA genes under the control of a native promoter were found to be capable of synthesizing mycosporine-2-glycine. The accumulation level of mycosporine-2-glycine was again higher under the high-salinity condition. In the transformed E. coli cells, its level was approximately 85.2 ± 0.7 μmol/g (dry weight). Successful production of a large amount of mycosporine in these cells provides a new opportunity in the search for an alternative natural sunscreen compound source.
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Physiological, biochemical and molecular responses of the halophilic cyanobacterium Aphanothece halophytica to Pi-deficiency
European Journal of Phycology, 2013Co-Authors: Keshwanand Tripathi, Hakuto Kageyama, Teruhiro Takabe, Naveen K. Sharma, Ashwani K. RaiAbstract:We studied the responses of a halophilic cyanobacterium Aphanothece halophytica at surplus (normal composition of growth medium containing 125 µM PO43−), sufficient (the minimum concentration supporting optimal growth, 22 µM PO43−) and deficient (no external supply of Pi) concentrations of inorganic phosphate (Pi). The cyanobacterium was able to grow well in Pi-deficient conditions until the end of incubation (14 days), though at a marginally reduced rate. The cellular P-quota in Pi-surplus cells at the end of incubation was 2.7 times that of their initial P-quota (0.75 µmol mg protein−1), and remained fairly high (0.442 µmol mg protein−1) even in Pi-deficient medium. However, cultures growing in Pi-sufficient medium (22 µM PO43−), upon transfer to Pi-deficient medium, exhibited a rapid decline in cellular P level. Furthermore, cells growing in Pi-surplus medium showed a rapid efflux of P into the external medium. Aphanothece halophytica exhibited a biphasic phosphate transport system involving both high-...
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halotolerant cyanobacterium Aphanothece halophytica contains an na dependent f1f0 atp synthase with a potential role in salt stress tolerance
Journal of Biological Chemistry, 2011Co-Authors: Hakuto Kageyama, Teruhiro Takabe, Aran Incharoensakdi, Worrawat Promden, Kanteera Soontharapirakkul, Nana Yamada, Atsuko IwamotokiharaAbstract:Aphanothece halophytica is a halotolerant alkaliphilic cyanobacterium that can grow in media of up to 3.0 m NaCl and pH 11. Here, we show that in addition to a typical H+-ATP synthase, Aphanothece halophytica contains a putative F1F0-type Na+-ATP synthase (ApNa+-ATPase) operon (ApNa+-atp). The operon consists of nine genes organized in the order of putative subunits β, ϵ, I, hypothetical protein, a, c, b, α, and γ. Homologous operons could also be found in some cyanobacteria such as Synechococcus sp. PCC 7002 and Acaryochloris marina MBIC11017. The ApNa+-atp operon was isolated from the A. halophytica genome and transferred into an Escherichia coli mutant DK8 (Δatp) deficient in ATP synthase. The inverted membrane vesicles of E. coli DK8 expressing ApNa+-ATPase exhibited Na+-dependent ATP hydrolysis activity, which was inhibited by monensin and tributyltin chloride, but not by the protonophore, carbonyl cyanide m-chlorophenyl hydrazone (CCCP). The Na+ ion protected the inhibition of ApNa+-ATPase by N,N′-dicyclohexylcarbodiimide. The ATP synthesis activity was also observed using the Na+-loaded inverted membrane vesicles. Expression of the ApNa+-atp operon in the heterologous cyanobacterium Synechococcus sp. PCC 7942 showed its localization in the cytoplasmic membrane fractions and increased tolerance to salt stress. These results indicate that A. halophytica has additional Na+-dependent F1F0-ATPase in the cytoplasmic membrane playing a potential role in salt-stress tolerance.
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Halotolerant cyanobacterium Aphanothece halophytica contains an Na+-dependent F1F0-ATP synthase with a potential role in salt-stress tolerance.
The Journal of biological chemistry, 2011Co-Authors: Kanteera Soontharapirakkul, Hakuto Kageyama, Aran Incharoensakdi, Worrawat Promden, Nana Yamada, Atsuko Iwamoto-kihara, Teruhiro TakabeAbstract:Aphanothece halophytica is a halotolerant alkaliphilic cyanobacterium that can grow in media of up to 3.0 m NaCl and pH 11. Here, we show that in addition to a typical H+-ATP synthase, Aphanothece halophytica contains a putative F1F0-type Na+-ATP synthase (ApNa+-ATPase) operon (ApNa+-atp). The operon consists of nine genes organized in the order of putative subunits β, ϵ, I, hypothetical protein, a, c, b, α, and γ. Homologous operons could also be found in some cyanobacteria such as Synechococcus sp. PCC 7002 and Acaryochloris marina MBIC11017. The ApNa+-atp operon was isolated from the A. halophytica genome and transferred into an Escherichia coli mutant DK8 (Δatp) deficient in ATP synthase. The inverted membrane vesicles of E. coli DK8 expressing ApNa+-ATPase exhibited Na+-dependent ATP hydrolysis activity, which was inhibited by monensin and tributyltin chloride, but not by the protonophore, carbonyl cyanide m-chlorophenyl hydrazone (CCCP). The Na+ ion protected the inhibition of ApNa+-ATPase by N,N′-dicyclohexylcarbodiimide. The ATP synthesis activity was also observed using the Na+-loaded inverted membrane vesicles. Expression of the ApNa+-atp operon in the heterologous cyanobacterium Synechococcus sp. PCC 7942 showed its localization in the cytoplasmic membrane fractions and increased tolerance to salt stress. These results indicate that A. halophytica has additional Na+-dependent F1F0-ATPase in the cytoplasmic membrane playing a potential role in salt-stress tolerance.