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Wan Fusheng - One of the best experts on this subject based on the ideXlab platform.

  • effects of solanum lyratum thumb extract on notch1 gene expression and Apoptosis in human stomach sgc 7901 cells
    Journal of Liaoning University of Traditional Chinese Medicine, 2011
    Co-Authors: Wan Fusheng
    Abstract:

    Objective:To investigate the effects of Solanum Lyratum Thunb Extract(SLT)on Apoptosis and the expression of Notch1,Caspase-9 genes in Human stomach cancer SGC-7901 cells.Methods:Dried whole herbs of Solanum Lyratum Thunb were extracted by boiling distilled water.Human Stomach Cancer SGC-7901 Cells were randomly divided into the control group and SLT-treated groups(12.5mg/mL,25mg/mL,50mg/mL),the growth inhibitory Rate was evaluated by MTT assay,cell Apoptosis Rate was determined by flow cytometry;Expression of Notch1,Caspase-9 mRNA were detected by semi-quantitive RT-PCR,Expression level of Notch1 and Caspase-9 proteins were detected by two-step immunhistochemical staining.Results:Compared with control group,the proliferation inhibitory Rate and Apoptosis Rate were increased obviously(P0.05),the expression of Notch1 mRNA and protein were obvious decreased respectively(P0.05),the expression of Caspase-9 mRNA and protein were obvious increased respectively(P0.05),and display a effect in a dose-dependent manner in the SGC-7901 cells of the SLT-treated groups.Conclusion:SLT can downregulate expression level of Notch1 and induce Apoptosis of the Human stomach cancer SGC-7901 cells.

  • study on Apoptosis of human stomach sgc 7901 cells induced by solanum lyratum thunb extract
    Lishizhen Medicine and Materia Medica Research, 2009
    Co-Authors: Wan Fusheng
    Abstract:

    Objective To investigate the effects of Solanum lyratum Thunb.Extract(SLT) on Apoptosis of human stomach cancer SGC-7901 cells.Methods Dried whole herbs of Solanum Lyratum Thunb were extracted by boiling distilled water.Human stomach cancer SGC-7901 cells were randomly divided into control group,SLT-treated groups(12.5 mg/ml,25 mg/ml,50 mg/ml) and positive control(25μg/ ml DDP).The growth inhibitory Rate was evaluated by MTT assay.Morphological changes of Apoptosis were observed with fluorescence microscope.Cell Apoptosis Rate was determined by flow cytometry and the express of bcl-xl,caspase-9,caspase-3 and bid mRNA were detected by semi-quantity RT-PCR.Results Compared with control group,the cell proliferation inhibitory Rate and Apoptosis Rate of Human stomach cancer SGC-7901 cells were increased obviously(P0.05) in SLT-treated groups.Morphology of SGC-7901 cells showed the nuclear shrinkage,chromatin condensation and margination,and no changes were observed in control group.The expression of bcl-xl mRNA was obviously decreased(P0.05),the expression of caspase-9,caspase-3 and bid mRNA were obviously increased respectively(P0.05),in a dose-dependent manner in SGC-7901 cells of the SLT-treated groups.Conclusion SLT can induce Apoptosis and inhibit proliferation of the human stomach cancer SGC-7901 cells,by regulating expression of bcl-xl,caspase-9,caspase-3 and bid genes.

  • influence of solanum lyratum thunb extract on Apoptosis and the expression of bcl xl bid genes in human stomach cancer sgc 7901 cells
    Chinese Traditional Patent Medicine, 2008
    Co-Authors: Wan Fusheng
    Abstract:

    AIM:To investigate the effects of Solanum lyratum Thunb Extract(SLT) on Apoptosis and the expression of bcl-xl,bid genes in human stomach cancer SGC-7901 cells.METHODS:Dried whole herbs of Solanum lyratum Thunb were extracted by boilingdistilled water.Human stomach cancer SGC-7901 cells were randomly divided into the control group and SLT-treated groups(12.5 mg/mL,25 mg/mL,50 mg/mL),cell Apoptosis Rate wase determined by flow cytometry.Expression of bcl-xl and bid mRNA were detected by semi-quantitive RT-PCR.Expression level of Bcl-xl and Bid proteins were detected by two-step immunhistochemical staining.RESULTS:Compared with control group,the proliferation inhibitory Rate and Apoptosis Rate increased obviously(P0.05).The expression of bcl-xl mRNA and Bcl-xl protein obviously decreased respectively(P0.05).The expression of bid mRNA and Bid protein obviously increased respectively(P0.05).CONCLUSION:SLT can induce Apoptosis and inhibit proliferation of the Human stomach cancer SGC-7901 cells,and display effect in a dose-dependent manner by regulating expression of bcl-xl and bid genes.

Haitao Guan - One of the best experts on this subject based on the ideXlab platform.

  • matrine induces Apoptosis in gastric carcinoma cells via alteration of fas fasl and activation of caspase 3
    Journal of Ethnopharmacology, 2009
    Co-Authors: Zhijun Dai, Jie Gao, Xijing Wang, Hongtao Ren, Xiaoxu Liu, Huafeng Kang, Haitao Guan
    Abstract:

    Abstract Aim of the study Matrine, an alkaloid purified from the chinese herb Sophora flavescens Ait, is well known to possess activities including anti-inflammation, anti-fibrotic and anticancer. In this study, the mechanism of matrine inducing the Apoptosis of gastric carcinoma cells was investigated. Materials and Methods Proliferation of SGC-7901 cells was examined by MTT assay. Cellular morphology was observed under transmission electron microscope. Flow cytometry (FCM) was used to observe the Apoptosis of SGC-7901 cells by staining with annexinV-FITC/PI. The expression levels of Fas/FasL in SGC-7901 cells were monitored by FCM analysis using an indirect immunoflurenscence method. Activity of caspase-3 enzyme was measured by spectrofluorometry. Results MTT assay showed that matrine inhibited SGC-7901 cells proliferation in a dose-dependent and time-dependent manner. Apoptosis induction was demonstRated by morphological changes under electron microscope and FCM analysis. Florescence intensity levels of Fas and FasL were found to be equally up-regulated after matrine treatment, which were both correlated with Apoptosis Rate. The activity of caspase-3 enzyme increased in matrine groups, positively correlated with Apoptosis Rate. Conclusions Matrine could inhibit cell proliferation and induce Apoptosis of SGC-7901 cells in vitro. The Apoptosis induction appears to proceed by up-regulating Fas/FasL expression and activating caspase-3 enzyme.

Rolf E Brenner - One of the best experts on this subject based on the ideXlab platform.

  • antioxidative therapy in an ex vivo human cartilage trauma model attenuation of trauma induced cell loss and ecm destructive enzymes by n acetyl cysteine
    Osteoarthritis and Cartilage, 2016
    Co-Authors: Jana Riegger, Helga Joos, Hansgeorg Palm, Benedikt Friemert, Heiko Reichel, Anita Ignatius, Rolf E Brenner
    Abstract:

    Summary Objective Mechanical trauma of articular cartilage results in cell loss and cytokine-driven inflammatory response. Subsequent accumulation of reactive oxygen (ROS) and nitrogen (RNS) species enhances the enzymatic degradation of the extracellular matrix (ECM). This study aims on the therapeutic potential of N-acetyl cysteine (NAC) in a human ex vivo cartilage trauma-model, focusing on cell- and chondroprotective features. Design Human full-thickness cartilage explants were subjected to a defined impact trauma (0.59 J) and treated with NAC. Efficiency of NAC administration was evaluated by following outcome parameters: cell viability, Apoptosis Rate, anabolic/catabolic gene expression, secretion and activity of matrix metalloproteinases (MMPs) and proteoglycan (PG) release. Results Continuous NAC administration increased cell viability and reduced the Apoptosis Rate after trauma. It also suppressed trauma-induced gene expression of ECM-destructive enzymes, such as ADAMTS-4, MMP-1, -2, -3 and -13 in a dosage- and time-depending manner. Subsequent suppression of MMP-2 and MMP-13 secretion reflected these findings on protein level. Moreover, NAC inhibited proteolytic activity of MMPs and reduced PG release. Conclusion In the context of this ex vivo study, we showed not only remarkable cell- and chondroprotective features, but also revealed new encouraging findings concerning the therapeutically effective concentration and treatment-time regimen of NAC. Its defense against chondrocyte Apoptosis and catabolic enzyme secretion recommends NAC as a multifunctional add-on reagent for pharmaceutical intervention after cartilage injury. Taken together, our data increase the knowledge on the therapeutic potential of NAC after cartilage trauma and presents a basis for future in vivo studies.

Juha P Vayrynen - One of the best experts on this subject based on the ideXlab platform.

  • putative anoikis resistant subpopulations in colorectal carcinoma a marker of adverse prognosis
    Apmis, 2020
    Co-Authors: Madhura Patankar, Taneli Mattila, Juha P Vayrynen, Kai Klintrup, Jyrki Makela
    Abstract:

    Anoikis is a form of Apoptosis induced when a cell loses contact with the extracellular matrix (ECM). Anoikis resistance is essential for metastasis formation, yet only detectable by in vitro experiments. We present a method for quantitation of putative anoikis-resistant (AR) subpopulations in colorectal carcinoma (CRC) and evaluate their prognostic significance. We studied 137 CRC cases and identified cell subpopulations with and without stromal or extracellular matrix (ECM) contact with hematoxylin-and-eosin-stained sections and immunohistochemistry for laminin and type IV collagen. Suprabasal cells of micropapillary structures and inner cells of cribriform and solid structures lacked both stromal contact and contact with ECM proteins. Apoptosis Rate (M30) was lower in these subpopulations than in the other carcinoma cells, consistent with putative AR subpopulation. We determined the areal density of these subpopulations (number/mm2 tumor tissue), and their high areal density independently indicates low cancer-specific survival. In conclusion, we show evidence that subpopulations of carcinoma cells in micropapillary, cribriform, and solid structures are resistant to anoikis as shown by lack of ECM contact and low Apoptosis Rate. Abundance of these subpopulations is a new independent indicator of poor prognosis in CRC, consistent with the importance of anoikis resistance in the formation of metastasis.

Madhura Patankar - One of the best experts on this subject based on the ideXlab platform.

  • putative anoikis resistant subpopulations in colorectal carcinoma a marker of adverse prognosis
    Apmis, 2020
    Co-Authors: Madhura Patankar, Taneli Mattila, Juha P Vayrynen, Kai Klintrup, Jyrki Makela
    Abstract:

    Anoikis is a form of Apoptosis induced when a cell loses contact with the extracellular matrix (ECM). Anoikis resistance is essential for metastasis formation, yet only detectable by in vitro experiments. We present a method for quantitation of putative anoikis-resistant (AR) subpopulations in colorectal carcinoma (CRC) and evaluate their prognostic significance. We studied 137 CRC cases and identified cell subpopulations with and without stromal or extracellular matrix (ECM) contact with hematoxylin-and-eosin-stained sections and immunohistochemistry for laminin and type IV collagen. Suprabasal cells of micropapillary structures and inner cells of cribriform and solid structures lacked both stromal contact and contact with ECM proteins. Apoptosis Rate (M30) was lower in these subpopulations than in the other carcinoma cells, consistent with putative AR subpopulation. We determined the areal density of these subpopulations (number/mm2 tumor tissue), and their high areal density independently indicates low cancer-specific survival. In conclusion, we show evidence that subpopulations of carcinoma cells in micropapillary, cribriform, and solid structures are resistant to anoikis as shown by lack of ECM contact and low Apoptosis Rate. Abundance of these subpopulations is a new independent indicator of poor prognosis in CRC, consistent with the importance of anoikis resistance in the formation of metastasis.