The Experts below are selected from a list of 186 Experts worldwide ranked by ideXlab platform
Stephen F Donovan - One of the best experts on this subject based on the ideXlab platform.
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lewisite metabolites in urine by solid phase extraction dual column reversed phase liquid chromatography isotope dilution tandem mass spectrometry
Journal of Analytical Toxicology, 2016Co-Authors: Jason Palcic, Janet S Jones, Lindsay E Flagg, Stephen F Donovan, Redentor A Salonga, Walter E Mock, Victor S AsirvathamAbstract:: Lewisite (2-chlorovinyldichloroarsine) is a chemical warfare agent developed during World War I. A quantitative method using solid phase extraction (SPE) followed by dual column liquid chromatography (LC)-isotope dilution tandem mass spectrometry (MS-MS) was developed for the determination of (2-chlorovinyl)arsonic Acid (CVAOA), a metabolite of Lewisite, in human urine. The sample was treated with hydrogen peroxide to oxidize any (2-chlorovinyl)arsonous Acid (CVAA) that remained in the trivalent arsenic oxidation state. There was 1.19% (arsenic purity) of bis-(2-chlorovinyl)Arsinic Acid (BCVAOA), a minor Lewisite metabolite, in the stock CVAA material. The high-throughput method qualitatively assessed BCVAOA simultaneously utilizing normal-phase silica SPE followed by reversed-phase C18 LC for an orthogonal separation. The chromatographic method results in a 5.8-min cycle time with adequate retention (k' = 2.4) of CVAOA. The mass spectrometer was operated in positive electrospray ionization mode with quantitative m/z 186.9→61.0 and confirmation 186.9→91.0 mass transitions. This selective method demonstrated linearity, accuracy and reproducibility for the clinically relevant calibration range (25-3,200 µg/L as CVAA). The method detection limit was 3.3 µg/L as CVAA from a 10 µL injection. This LC-MS-MS emergency response method has a throughput of >240 samples (2.5 extracted 96-well plates) per day.
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lewisite exposure biomarkers in urine by liquid chromatography inductively coupled plasma tandem mass spectrometry with an accelerated matrix matched stability study
Journal of Analytical Atomic Spectrometry, 2015Co-Authors: Jason Palcic, Janet S Jones, Lindsay E Flagg, Stephen F DonovanAbstract:A simple and robust LC-ICP-MS/MS method is described for quantitative analysis of human urine for (2-chlorovinyl)arsonic Acid (CVAOA), a metabolite of Lewisite. This method oxidizes (2-chlorovinyl)arsenous Acid (CVAA) with the addition of hydrogen peroxide to measure total Lewisite-1 metabolites as CVAOA, with m/z 75 → 91 detection specific for arsenic. The percentage of CVAA to CVAOA is clinically insignificant, because the amount of CVAA conversion to CVAOA is dependent upon residence time in the body. Once excreted into the urine, conversion of CVAA to CVAOA is dependent upon temperature and oxidative potential of the urine. The method also allowed for qualitative analysis for bis(2-chlorovinyl)Arsinic Acid (BCVAOA) and (1-chlorovinyl)arsonic Acid (gem-CVAOA), minor Lewisite metabolites. Traditional methods have ignored these minor metabolites; the bis-metabolites can comprise ∼30% of total Lewisite metabolites from chemical munitions and must be accounted for in the exposure measurement. The ion-pairing chromatography method results in a 5.73 min injection-to-injection cycle time with adequate retention (k′ = 2.9) of CVAOA. The weighted (1/x2) linear least squares regression results have correlation coefficients (r2 > 0.998) for the clinically relevant calibration range of 50–3500 μg L−1. The selectivity of the method is measured by chromatographic resolution from other common arsenic compounds that may interfere with the analysis. The 96-well plate preparation of 0.1 mL sample of human urine results in a method detection limit of 2.2 μg L−1. Quantitative results from proficiency testing specimens demonstrate the accuracy (−7.1 to +4.3%) of the method. Quality control data demonstrate inter-analyst precise (3.1 to 3.3%) quantitative results of the method. An accelerated Arrhenius matrix-matched stability study demonstrates Lewisite metabolites are stabile in urine far greater than a year. The trivalent arsenic, CVAA oxidation half-life is estimated at normal body temperature in vitro at 6.2 days. The combined sample preparation and analysis portions of this emergency response method have a throughput of 250 samples per day.
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Lewisite exposure biomarkers in urine by liquid chromatography – inductively coupled plasma tandem mass spectrometry: with an accelerated matrix-matched stability study
Journal of Analytical Atomic Spectrometry, 2015Co-Authors: Jason Palcic, Janet S Jones, E. Lindsay Flagg, Stephen F DonovanAbstract:A simple and robust LC-ICP-MS/MS method is described for quantitative analysis of human urine for (2-chlorovinyl)arsonic Acid (CVAOA), a metabolite of Lewisite. This method oxidizes (2-chlorovinyl)arsenous Acid (CVAA) with the addition of hydrogen peroxide to measure total Lewisite-1 metabolites as CVAOA, with m/z 75 → 91 detection specific for arsenic. The percentage of CVAA to CVAOA is clinically insignificant, because the amount of CVAA conversion to CVAOA is dependent upon residence time in the body. Once excreted into the urine, conversion of CVAA to CVAOA is dependent upon temperature and oxidative potential of the urine. The method also allowed for qualitative analysis for bis(2-chlorovinyl)Arsinic Acid (BCVAOA) and (1-chlorovinyl)arsonic Acid (gem-CVAOA), minor Lewisite metabolites. Traditional methods have ignored these minor metabolites; the bis-metabolites can comprise ∼30% of total Lewisite metabolites from chemical munitions and must be accounted for in the exposure measurement. The ion-pairing chromatography method results in a 5.73 min injection-to-injection cycle time with adequate retention (k′ = 2.9) of CVAOA. The weighted (1/x2) linear least squares regression results have correlation coefficients (r2 > 0.998) for the clinically relevant calibration range of 50–3500 μg L−1. The selectivity of the method is measured by chromatographic resolution from other common arsenic compounds that may interfere with the analysis. The 96-well plate preparation of 0.1 mL sample of human urine results in a method detection limit of 2.2 μg L−1. Quantitative results from proficiency testing specimens demonstrate the accuracy (−7.1 to +4.3%) of the method. Quality control data demonstrate inter-analyst precise (3.1 to 3.3%) quantitative results of the method. An accelerated Arrhenius matrix-matched stability study demonstrates Lewisite metabolites are stabile in urine far greater than a year. The trivalent arsenic, CVAA oxidation half-life is estimated at normal body temperature in vitro at 6.2 days. The combined sample preparation and analysis portions of this emergency response method have a throughput of 250 samples per day.
Jason Palcic - One of the best experts on this subject based on the ideXlab platform.
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lewisite metabolites in urine by solid phase extraction dual column reversed phase liquid chromatography isotope dilution tandem mass spectrometry
Journal of Analytical Toxicology, 2016Co-Authors: Jason Palcic, Janet S Jones, Lindsay E Flagg, Stephen F Donovan, Redentor A Salonga, Walter E Mock, Victor S AsirvathamAbstract:: Lewisite (2-chlorovinyldichloroarsine) is a chemical warfare agent developed during World War I. A quantitative method using solid phase extraction (SPE) followed by dual column liquid chromatography (LC)-isotope dilution tandem mass spectrometry (MS-MS) was developed for the determination of (2-chlorovinyl)arsonic Acid (CVAOA), a metabolite of Lewisite, in human urine. The sample was treated with hydrogen peroxide to oxidize any (2-chlorovinyl)arsonous Acid (CVAA) that remained in the trivalent arsenic oxidation state. There was 1.19% (arsenic purity) of bis-(2-chlorovinyl)Arsinic Acid (BCVAOA), a minor Lewisite metabolite, in the stock CVAA material. The high-throughput method qualitatively assessed BCVAOA simultaneously utilizing normal-phase silica SPE followed by reversed-phase C18 LC for an orthogonal separation. The chromatographic method results in a 5.8-min cycle time with adequate retention (k' = 2.4) of CVAOA. The mass spectrometer was operated in positive electrospray ionization mode with quantitative m/z 186.9→61.0 and confirmation 186.9→91.0 mass transitions. This selective method demonstrated linearity, accuracy and reproducibility for the clinically relevant calibration range (25-3,200 µg/L as CVAA). The method detection limit was 3.3 µg/L as CVAA from a 10 µL injection. This LC-MS-MS emergency response method has a throughput of >240 samples (2.5 extracted 96-well plates) per day.
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lewisite exposure biomarkers in urine by liquid chromatography inductively coupled plasma tandem mass spectrometry with an accelerated matrix matched stability study
Journal of Analytical Atomic Spectrometry, 2015Co-Authors: Jason Palcic, Janet S Jones, Lindsay E Flagg, Stephen F DonovanAbstract:A simple and robust LC-ICP-MS/MS method is described for quantitative analysis of human urine for (2-chlorovinyl)arsonic Acid (CVAOA), a metabolite of Lewisite. This method oxidizes (2-chlorovinyl)arsenous Acid (CVAA) with the addition of hydrogen peroxide to measure total Lewisite-1 metabolites as CVAOA, with m/z 75 → 91 detection specific for arsenic. The percentage of CVAA to CVAOA is clinically insignificant, because the amount of CVAA conversion to CVAOA is dependent upon residence time in the body. Once excreted into the urine, conversion of CVAA to CVAOA is dependent upon temperature and oxidative potential of the urine. The method also allowed for qualitative analysis for bis(2-chlorovinyl)Arsinic Acid (BCVAOA) and (1-chlorovinyl)arsonic Acid (gem-CVAOA), minor Lewisite metabolites. Traditional methods have ignored these minor metabolites; the bis-metabolites can comprise ∼30% of total Lewisite metabolites from chemical munitions and must be accounted for in the exposure measurement. The ion-pairing chromatography method results in a 5.73 min injection-to-injection cycle time with adequate retention (k′ = 2.9) of CVAOA. The weighted (1/x2) linear least squares regression results have correlation coefficients (r2 > 0.998) for the clinically relevant calibration range of 50–3500 μg L−1. The selectivity of the method is measured by chromatographic resolution from other common arsenic compounds that may interfere with the analysis. The 96-well plate preparation of 0.1 mL sample of human urine results in a method detection limit of 2.2 μg L−1. Quantitative results from proficiency testing specimens demonstrate the accuracy (−7.1 to +4.3%) of the method. Quality control data demonstrate inter-analyst precise (3.1 to 3.3%) quantitative results of the method. An accelerated Arrhenius matrix-matched stability study demonstrates Lewisite metabolites are stabile in urine far greater than a year. The trivalent arsenic, CVAA oxidation half-life is estimated at normal body temperature in vitro at 6.2 days. The combined sample preparation and analysis portions of this emergency response method have a throughput of 250 samples per day.
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Lewisite exposure biomarkers in urine by liquid chromatography – inductively coupled plasma tandem mass spectrometry: with an accelerated matrix-matched stability study
Journal of Analytical Atomic Spectrometry, 2015Co-Authors: Jason Palcic, Janet S Jones, E. Lindsay Flagg, Stephen F DonovanAbstract:A simple and robust LC-ICP-MS/MS method is described for quantitative analysis of human urine for (2-chlorovinyl)arsonic Acid (CVAOA), a metabolite of Lewisite. This method oxidizes (2-chlorovinyl)arsenous Acid (CVAA) with the addition of hydrogen peroxide to measure total Lewisite-1 metabolites as CVAOA, with m/z 75 → 91 detection specific for arsenic. The percentage of CVAA to CVAOA is clinically insignificant, because the amount of CVAA conversion to CVAOA is dependent upon residence time in the body. Once excreted into the urine, conversion of CVAA to CVAOA is dependent upon temperature and oxidative potential of the urine. The method also allowed for qualitative analysis for bis(2-chlorovinyl)Arsinic Acid (BCVAOA) and (1-chlorovinyl)arsonic Acid (gem-CVAOA), minor Lewisite metabolites. Traditional methods have ignored these minor metabolites; the bis-metabolites can comprise ∼30% of total Lewisite metabolites from chemical munitions and must be accounted for in the exposure measurement. The ion-pairing chromatography method results in a 5.73 min injection-to-injection cycle time with adequate retention (k′ = 2.9) of CVAOA. The weighted (1/x2) linear least squares regression results have correlation coefficients (r2 > 0.998) for the clinically relevant calibration range of 50–3500 μg L−1. The selectivity of the method is measured by chromatographic resolution from other common arsenic compounds that may interfere with the analysis. The 96-well plate preparation of 0.1 mL sample of human urine results in a method detection limit of 2.2 μg L−1. Quantitative results from proficiency testing specimens demonstrate the accuracy (−7.1 to +4.3%) of the method. Quality control data demonstrate inter-analyst precise (3.1 to 3.3%) quantitative results of the method. An accelerated Arrhenius matrix-matched stability study demonstrates Lewisite metabolites are stabile in urine far greater than a year. The trivalent arsenic, CVAA oxidation half-life is estimated at normal body temperature in vitro at 6.2 days. The combined sample preparation and analysis portions of this emergency response method have a throughput of 250 samples per day.
Janet S Jones - One of the best experts on this subject based on the ideXlab platform.
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lewisite metabolites in urine by solid phase extraction dual column reversed phase liquid chromatography isotope dilution tandem mass spectrometry
Journal of Analytical Toxicology, 2016Co-Authors: Jason Palcic, Janet S Jones, Lindsay E Flagg, Stephen F Donovan, Redentor A Salonga, Walter E Mock, Victor S AsirvathamAbstract:: Lewisite (2-chlorovinyldichloroarsine) is a chemical warfare agent developed during World War I. A quantitative method using solid phase extraction (SPE) followed by dual column liquid chromatography (LC)-isotope dilution tandem mass spectrometry (MS-MS) was developed for the determination of (2-chlorovinyl)arsonic Acid (CVAOA), a metabolite of Lewisite, in human urine. The sample was treated with hydrogen peroxide to oxidize any (2-chlorovinyl)arsonous Acid (CVAA) that remained in the trivalent arsenic oxidation state. There was 1.19% (arsenic purity) of bis-(2-chlorovinyl)Arsinic Acid (BCVAOA), a minor Lewisite metabolite, in the stock CVAA material. The high-throughput method qualitatively assessed BCVAOA simultaneously utilizing normal-phase silica SPE followed by reversed-phase C18 LC for an orthogonal separation. The chromatographic method results in a 5.8-min cycle time with adequate retention (k' = 2.4) of CVAOA. The mass spectrometer was operated in positive electrospray ionization mode with quantitative m/z 186.9→61.0 and confirmation 186.9→91.0 mass transitions. This selective method demonstrated linearity, accuracy and reproducibility for the clinically relevant calibration range (25-3,200 µg/L as CVAA). The method detection limit was 3.3 µg/L as CVAA from a 10 µL injection. This LC-MS-MS emergency response method has a throughput of >240 samples (2.5 extracted 96-well plates) per day.
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lewisite exposure biomarkers in urine by liquid chromatography inductively coupled plasma tandem mass spectrometry with an accelerated matrix matched stability study
Journal of Analytical Atomic Spectrometry, 2015Co-Authors: Jason Palcic, Janet S Jones, Lindsay E Flagg, Stephen F DonovanAbstract:A simple and robust LC-ICP-MS/MS method is described for quantitative analysis of human urine for (2-chlorovinyl)arsonic Acid (CVAOA), a metabolite of Lewisite. This method oxidizes (2-chlorovinyl)arsenous Acid (CVAA) with the addition of hydrogen peroxide to measure total Lewisite-1 metabolites as CVAOA, with m/z 75 → 91 detection specific for arsenic. The percentage of CVAA to CVAOA is clinically insignificant, because the amount of CVAA conversion to CVAOA is dependent upon residence time in the body. Once excreted into the urine, conversion of CVAA to CVAOA is dependent upon temperature and oxidative potential of the urine. The method also allowed for qualitative analysis for bis(2-chlorovinyl)Arsinic Acid (BCVAOA) and (1-chlorovinyl)arsonic Acid (gem-CVAOA), minor Lewisite metabolites. Traditional methods have ignored these minor metabolites; the bis-metabolites can comprise ∼30% of total Lewisite metabolites from chemical munitions and must be accounted for in the exposure measurement. The ion-pairing chromatography method results in a 5.73 min injection-to-injection cycle time with adequate retention (k′ = 2.9) of CVAOA. The weighted (1/x2) linear least squares regression results have correlation coefficients (r2 > 0.998) for the clinically relevant calibration range of 50–3500 μg L−1. The selectivity of the method is measured by chromatographic resolution from other common arsenic compounds that may interfere with the analysis. The 96-well plate preparation of 0.1 mL sample of human urine results in a method detection limit of 2.2 μg L−1. Quantitative results from proficiency testing specimens demonstrate the accuracy (−7.1 to +4.3%) of the method. Quality control data demonstrate inter-analyst precise (3.1 to 3.3%) quantitative results of the method. An accelerated Arrhenius matrix-matched stability study demonstrates Lewisite metabolites are stabile in urine far greater than a year. The trivalent arsenic, CVAA oxidation half-life is estimated at normal body temperature in vitro at 6.2 days. The combined sample preparation and analysis portions of this emergency response method have a throughput of 250 samples per day.
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Lewisite exposure biomarkers in urine by liquid chromatography – inductively coupled plasma tandem mass spectrometry: with an accelerated matrix-matched stability study
Journal of Analytical Atomic Spectrometry, 2015Co-Authors: Jason Palcic, Janet S Jones, E. Lindsay Flagg, Stephen F DonovanAbstract:A simple and robust LC-ICP-MS/MS method is described for quantitative analysis of human urine for (2-chlorovinyl)arsonic Acid (CVAOA), a metabolite of Lewisite. This method oxidizes (2-chlorovinyl)arsenous Acid (CVAA) with the addition of hydrogen peroxide to measure total Lewisite-1 metabolites as CVAOA, with m/z 75 → 91 detection specific for arsenic. The percentage of CVAA to CVAOA is clinically insignificant, because the amount of CVAA conversion to CVAOA is dependent upon residence time in the body. Once excreted into the urine, conversion of CVAA to CVAOA is dependent upon temperature and oxidative potential of the urine. The method also allowed for qualitative analysis for bis(2-chlorovinyl)Arsinic Acid (BCVAOA) and (1-chlorovinyl)arsonic Acid (gem-CVAOA), minor Lewisite metabolites. Traditional methods have ignored these minor metabolites; the bis-metabolites can comprise ∼30% of total Lewisite metabolites from chemical munitions and must be accounted for in the exposure measurement. The ion-pairing chromatography method results in a 5.73 min injection-to-injection cycle time with adequate retention (k′ = 2.9) of CVAOA. The weighted (1/x2) linear least squares regression results have correlation coefficients (r2 > 0.998) for the clinically relevant calibration range of 50–3500 μg L−1. The selectivity of the method is measured by chromatographic resolution from other common arsenic compounds that may interfere with the analysis. The 96-well plate preparation of 0.1 mL sample of human urine results in a method detection limit of 2.2 μg L−1. Quantitative results from proficiency testing specimens demonstrate the accuracy (−7.1 to +4.3%) of the method. Quality control data demonstrate inter-analyst precise (3.1 to 3.3%) quantitative results of the method. An accelerated Arrhenius matrix-matched stability study demonstrates Lewisite metabolites are stabile in urine far greater than a year. The trivalent arsenic, CVAA oxidation half-life is estimated at normal body temperature in vitro at 6.2 days. The combined sample preparation and analysis portions of this emergency response method have a throughput of 250 samples per day.
Olujide Akinbo - One of the best experts on this subject based on the ideXlab platform.
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Development and Application of a Robust Speciation Method for Determination of Six Arsenic Compounds Present in Human Urine
2013Co-Authors: Lisa S. Milstein, Amal Essader, Edo D. Pellizzari, Reshan A. Fern, James H. Raymer, Keith E. Levine, Olujide AkinboAbstract:dimethyl Arsinic Acid] present in human urine were determined using ion-exchange chromatography combined with inductively coupled plasma mass spectrometry (IC-ICP-MS). Baseline separation was achieved for all six species as well as for the internal standard (potassium hexahydroxy antimonate V) in a single chromatographic run of less than 30 min, using an ammonium carbonate buffer gradient (between 10 and 50 mM) at ambient temperature, in conjunction with cation- and anion-exchange columns in series. The performance of the method was evaluated with respect to linearity, precision, accuracy, and detection limits. This method was applied to determine the concentration of these six arsenic species in human urine samples (n = 251) collected from a population-based exposure assessment survey. Method precision was demonstrated by the analysis of duplicate samples that were prepared over a 2-year analysis period. Total arsenic was also determined for the urine samples using flow injection analysis coupled to ICP-MS. The summed concentration of the arsenic species was compared with the measured arsenic total to demonstrate mass balance. Key words: arsenic, HPLC, ion chromatography, plasma mass spectrometry, speciation, urine. Environ Health Perspect 111:293–296 (2003). doi:10.1289/ehp.5525 available vi
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Selection of a suitable mobile phase for the speciation of four arsenic compounds in drinking water samples using ion-exchange chromatography coupled to inductively coupled plasma mass spectrometry.
Environment International, 2002Co-Authors: Lisa S. Milstein, Amal Essader, Edo D. Pellizzari, Reshan A. Fernando, Olujide AkinboAbstract:Abstract The performance of two mobile phase buffers, phosphate and TRIS, were compared for the speciation of four arsenic species: arsenate (As(V)), arsenite (As(III)), mono methylarsonic Acid (MMA), and dimethyl Arsinic Acid (DMA) in drinking water, using ion-exchange chromatography inductivelycoupled plasma mass spectrometry (IEC-ICP-MS). The mobile phase containing TRIS acetate buffer (“TRIS”) demonstrated superior performance in baseline separation of all four arsenic species and the internal standard. It is also applicable to high-throughput sample analysis as it minimized the frequency required to clean the sampling interface due to salt build-up when compared to the phosphate mobile phase. The method was evaluated for its precision, accuracy, linearity and detection limits. The method was successfully applied for the analysis of drinking water samples.
Lindsay E Flagg - One of the best experts on this subject based on the ideXlab platform.
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lewisite metabolites in urine by solid phase extraction dual column reversed phase liquid chromatography isotope dilution tandem mass spectrometry
Journal of Analytical Toxicology, 2016Co-Authors: Jason Palcic, Janet S Jones, Lindsay E Flagg, Stephen F Donovan, Redentor A Salonga, Walter E Mock, Victor S AsirvathamAbstract:: Lewisite (2-chlorovinyldichloroarsine) is a chemical warfare agent developed during World War I. A quantitative method using solid phase extraction (SPE) followed by dual column liquid chromatography (LC)-isotope dilution tandem mass spectrometry (MS-MS) was developed for the determination of (2-chlorovinyl)arsonic Acid (CVAOA), a metabolite of Lewisite, in human urine. The sample was treated with hydrogen peroxide to oxidize any (2-chlorovinyl)arsonous Acid (CVAA) that remained in the trivalent arsenic oxidation state. There was 1.19% (arsenic purity) of bis-(2-chlorovinyl)Arsinic Acid (BCVAOA), a minor Lewisite metabolite, in the stock CVAA material. The high-throughput method qualitatively assessed BCVAOA simultaneously utilizing normal-phase silica SPE followed by reversed-phase C18 LC for an orthogonal separation. The chromatographic method results in a 5.8-min cycle time with adequate retention (k' = 2.4) of CVAOA. The mass spectrometer was operated in positive electrospray ionization mode with quantitative m/z 186.9→61.0 and confirmation 186.9→91.0 mass transitions. This selective method demonstrated linearity, accuracy and reproducibility for the clinically relevant calibration range (25-3,200 µg/L as CVAA). The method detection limit was 3.3 µg/L as CVAA from a 10 µL injection. This LC-MS-MS emergency response method has a throughput of >240 samples (2.5 extracted 96-well plates) per day.
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lewisite exposure biomarkers in urine by liquid chromatography inductively coupled plasma tandem mass spectrometry with an accelerated matrix matched stability study
Journal of Analytical Atomic Spectrometry, 2015Co-Authors: Jason Palcic, Janet S Jones, Lindsay E Flagg, Stephen F DonovanAbstract:A simple and robust LC-ICP-MS/MS method is described for quantitative analysis of human urine for (2-chlorovinyl)arsonic Acid (CVAOA), a metabolite of Lewisite. This method oxidizes (2-chlorovinyl)arsenous Acid (CVAA) with the addition of hydrogen peroxide to measure total Lewisite-1 metabolites as CVAOA, with m/z 75 → 91 detection specific for arsenic. The percentage of CVAA to CVAOA is clinically insignificant, because the amount of CVAA conversion to CVAOA is dependent upon residence time in the body. Once excreted into the urine, conversion of CVAA to CVAOA is dependent upon temperature and oxidative potential of the urine. The method also allowed for qualitative analysis for bis(2-chlorovinyl)Arsinic Acid (BCVAOA) and (1-chlorovinyl)arsonic Acid (gem-CVAOA), minor Lewisite metabolites. Traditional methods have ignored these minor metabolites; the bis-metabolites can comprise ∼30% of total Lewisite metabolites from chemical munitions and must be accounted for in the exposure measurement. The ion-pairing chromatography method results in a 5.73 min injection-to-injection cycle time with adequate retention (k′ = 2.9) of CVAOA. The weighted (1/x2) linear least squares regression results have correlation coefficients (r2 > 0.998) for the clinically relevant calibration range of 50–3500 μg L−1. The selectivity of the method is measured by chromatographic resolution from other common arsenic compounds that may interfere with the analysis. The 96-well plate preparation of 0.1 mL sample of human urine results in a method detection limit of 2.2 μg L−1. Quantitative results from proficiency testing specimens demonstrate the accuracy (−7.1 to +4.3%) of the method. Quality control data demonstrate inter-analyst precise (3.1 to 3.3%) quantitative results of the method. An accelerated Arrhenius matrix-matched stability study demonstrates Lewisite metabolites are stabile in urine far greater than a year. The trivalent arsenic, CVAA oxidation half-life is estimated at normal body temperature in vitro at 6.2 days. The combined sample preparation and analysis portions of this emergency response method have a throughput of 250 samples per day.