The Experts below are selected from a list of 8121 Experts worldwide ranked by ideXlab platform
Hail Z. Rihan - One of the best experts on this subject based on the ideXlab platform.
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upregulation of cbf dreb1 and cold tolerance in Artificial Seeds of cauliflower brassica oleracea var botrytis
Scientia Horticulturae, 2017Co-Authors: Hail Z. Rihan, Mohammed Alissawi, M P FullerAbstract:Abstract An effective protocol for cauliflower micropropagation and Artificial seed production was optimized and applied in this study. However, in order to be a viable alternative to traditional Seeds, cauliflower Artificial Seeds need to show a high capacity to withstand abiotic stresses such as cold and desiccation. Therefore, in order to increase cauliflower abiotic stress tolerance, the effect of cold acclimation and drought on the cold tolerance of both cauliflower microshoots and mature plants were investigated. Moreover, the effect of cold and drought treatments on the induction of CBF/DREB1 gene regulation was tested. Both cold acclimation and drought improved the cold tolerance in both cauliflower microshoots and mature plants. However, whilst cold acclimation up-regulated CBF/DREB1 in cauliflower mature plants and microshoots, drought had the capacity only to up-regulate this gene in mature plants. Therefore, the high effect of cauliflower developmental stage on the CBF/DREB1 regulation was confirmed. Moreover, a small reduction in soil moisture had the capacity to unregulated this gene in mature cauliflower plants. The results presented in this study have an important role in the improvement of cauliflower micropropagation and the effectiveness of the Artificial seed production protocol. Furthermore, the results contribute to an understanding of the cold tolerance mechanism in Brassica oleraceae var botrytis.
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Artificial Seeds principle aspects and applications
Agronomy, 2017Co-Authors: Hail Z. Rihan, Fakhriya Kareem, Mohammed Elsayed Elmahrouk, M P FullerAbstract:Artificial Seeds are Artificially encapsulated somatic embryos (usually) or other vegetative parts such as shoot buds, cell aggregates, auxiliary buds, or any other micropropagules which can be sown as a seed and converted into a plant under in vitro or in vivo conditions. An improved Artificial seed production technique is considered a valuable alternate technology of propagation in many commercially important crops and a significant method for mass propagation of elite plant genotypes. The production of plant clones multiplied by tissue culture and distributed as Artificial Seeds could be a useful alternative to the costly F1 hybrids for different plant crops. The delivery of Artificial Seeds also facilitates issues such as undertaking several ways for scaling up in vitro cultures and acclimatization to ex vitro conditions. The development of an Artificial seed technique also provides a great approach for the improvement of various plant species such as trees and crops.
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The effect of molybdenum on the molecular control of cold tolerance in cauliflower (Brassica oleracea var. botrytis) Artificial Seeds
Plant Cell Tissue and Organ Culture (PCTOC), 2014Co-Authors: Hail Z. Rihan, Mohammed Al-issawi, Magda Al Shamari, Wondwossen Abate Woldie, Michele Kiernan, Michael P. FullerAbstract:Molybdenum (Mo) was used to improve the cold tolerance of cauliflower microshoots/Artificial Seeds. The optimal stage to introduce Mo to the micropropagation system was found to be the microshoots liquid culture stage, since its use within Artificial seed capsules or conversion media had negative effects on conversion rate and viability. Mo was found to improve the cold tolerance of both acclimated and non-acclimated microshoots/Artificial Seeds. The capacity of Mo to up-regulate CBF/DREB1 in cauliflower microshoots was confirmed. Moreover, this study is the first to confirm the ability of this element to up-regulate CBF/DREB1 without any low temperature treatment. Mo significantly increased the accumulation of 23 kDa polypeptide when it was used at 15 ppm concentration. However, Mo had a negative effect on the accumulation of dehydrin proteins which suggest that this group of proteins have no significant role in the cold tolerance of cauliflower microshoots. The study could help in improving the understanding of the abiotic stress network in plants and in improving the quality and efficiency of cauliflower Artificial seed production systems.
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encapsulation of cauliflower brassica oleracea var botrytis microshoots as Artificial Seeds and their conversion and growth in commercial substrates
Plant Cell Tissue and Organ Culture, 2011Co-Authors: Hail Z. Rihan, Mohammed Alissawi, S Burchett, M P FullerAbstract:An effective protocol for the mass production of cauliflower microshoots was refined using the meristematic layer of cauliflower curd. After the meristematic layer was surface sterilized and shaved off, a commercial blender was used for homogenization and several blending treatments were tested in the range 15–120 s and 30 s was found to be optimal in terms of the amount explants produced and their subsequent growth ability. Explants were cultivated in S23 liquid medium (4.4 g L−1 MS (Murashige and Skoog) and 3% v/w sucrose) supplemented with several combinations of plant growth regulators (PGRs) including 1 and 2 mg L−1 of Kinetin in combination with three types of auxins (indole butyric acid (IBA), Naphthaleneacetic acid (NAA) and Indole-3-acetic acid (IAA)), each at 1 and 2 mg L−1 concentration. The use of 2 mg L−1 Kinetin and 1 mg L−1 IBA gave the best results in terms of its effects on explant induction. Microshoots of different sizes were encapsulated in a sodium alginate matrix and the optimal stage suitable for the production of Artificial Seeds was assessed in terms of both subsequent conversion and plantlet viability. The feasibility of cultivating cauliflower Artificial Seeds in commercial substrates (compost, vermiculite, perlite and sand) irrigated with different solution mixtures including sterilized distilled water (SDW), PGRs-free S23 medium and S23 medium supplemented with Kinetin (1 and 2 mg L−1) and IBA or NAA at (1 and 2 mg L−1) was investigated. The use of 2 mg L−1 Kinetin and 2 mg L−1 NAA applied with S23 gave the optimal response with both perlite and compost. This study showed high growth capacity of cauliflower Artificial Seeds in commercial substrates which is considered a promising step for their direct use in vivo.
M P Fuller - One of the best experts on this subject based on the ideXlab platform.
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upregulation of cbf dreb1 and cold tolerance in Artificial Seeds of cauliflower brassica oleracea var botrytis
Scientia Horticulturae, 2017Co-Authors: Hail Z. Rihan, Mohammed Alissawi, M P FullerAbstract:Abstract An effective protocol for cauliflower micropropagation and Artificial seed production was optimized and applied in this study. However, in order to be a viable alternative to traditional Seeds, cauliflower Artificial Seeds need to show a high capacity to withstand abiotic stresses such as cold and desiccation. Therefore, in order to increase cauliflower abiotic stress tolerance, the effect of cold acclimation and drought on the cold tolerance of both cauliflower microshoots and mature plants were investigated. Moreover, the effect of cold and drought treatments on the induction of CBF/DREB1 gene regulation was tested. Both cold acclimation and drought improved the cold tolerance in both cauliflower microshoots and mature plants. However, whilst cold acclimation up-regulated CBF/DREB1 in cauliflower mature plants and microshoots, drought had the capacity only to up-regulate this gene in mature plants. Therefore, the high effect of cauliflower developmental stage on the CBF/DREB1 regulation was confirmed. Moreover, a small reduction in soil moisture had the capacity to unregulated this gene in mature cauliflower plants. The results presented in this study have an important role in the improvement of cauliflower micropropagation and the effectiveness of the Artificial seed production protocol. Furthermore, the results contribute to an understanding of the cold tolerance mechanism in Brassica oleraceae var botrytis.
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Artificial Seeds principle aspects and applications
Agronomy, 2017Co-Authors: Hail Z. Rihan, Fakhriya Kareem, Mohammed Elsayed Elmahrouk, M P FullerAbstract:Artificial Seeds are Artificially encapsulated somatic embryos (usually) or other vegetative parts such as shoot buds, cell aggregates, auxiliary buds, or any other micropropagules which can be sown as a seed and converted into a plant under in vitro or in vivo conditions. An improved Artificial seed production technique is considered a valuable alternate technology of propagation in many commercially important crops and a significant method for mass propagation of elite plant genotypes. The production of plant clones multiplied by tissue culture and distributed as Artificial Seeds could be a useful alternative to the costly F1 hybrids for different plant crops. The delivery of Artificial Seeds also facilitates issues such as undertaking several ways for scaling up in vitro cultures and acclimatization to ex vitro conditions. The development of an Artificial seed technique also provides a great approach for the improvement of various plant species such as trees and crops.
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encapsulation of cauliflower brassica oleracea var botrytis microshoots as Artificial Seeds and their conversion and growth in commercial substrates
Plant Cell Tissue and Organ Culture, 2011Co-Authors: Hail Z. Rihan, Mohammed Alissawi, S Burchett, M P FullerAbstract:An effective protocol for the mass production of cauliflower microshoots was refined using the meristematic layer of cauliflower curd. After the meristematic layer was surface sterilized and shaved off, a commercial blender was used for homogenization and several blending treatments were tested in the range 15–120 s and 30 s was found to be optimal in terms of the amount explants produced and their subsequent growth ability. Explants were cultivated in S23 liquid medium (4.4 g L−1 MS (Murashige and Skoog) and 3% v/w sucrose) supplemented with several combinations of plant growth regulators (PGRs) including 1 and 2 mg L−1 of Kinetin in combination with three types of auxins (indole butyric acid (IBA), Naphthaleneacetic acid (NAA) and Indole-3-acetic acid (IAA)), each at 1 and 2 mg L−1 concentration. The use of 2 mg L−1 Kinetin and 1 mg L−1 IBA gave the best results in terms of its effects on explant induction. Microshoots of different sizes were encapsulated in a sodium alginate matrix and the optimal stage suitable for the production of Artificial Seeds was assessed in terms of both subsequent conversion and plantlet viability. The feasibility of cultivating cauliflower Artificial Seeds in commercial substrates (compost, vermiculite, perlite and sand) irrigated with different solution mixtures including sterilized distilled water (SDW), PGRs-free S23 medium and S23 medium supplemented with Kinetin (1 and 2 mg L−1) and IBA or NAA at (1 and 2 mg L−1) was investigated. The use of 2 mg L−1 Kinetin and 2 mg L−1 NAA applied with S23 gave the optimal response with both perlite and compost. This study showed high growth capacity of cauliflower Artificial Seeds in commercial substrates which is considered a promising step for their direct use in vivo.
Mohammad Anis - One of the best experts on this subject based on the ideXlab platform.
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nutrient alginate encapsulation of nodal segments of althaea officinalis l for short term conservation and germplasm exchange
Plant Biosystems, 2018Co-Authors: Mohammad Anis, Abdulrahman A Alatar, Altaf Ahmad, Afshan NaazAbstract:AbstractIn the present study, an alternate method for germplasm storage in the form of Artificial Seeds was standardized via nodal explants excised from in vitro proliferated shoots. The explants were encapsulated using sodium alginate and calcium chloride as gelling matrix. For development of root along with shoot, excised nodal segments were pretreated with ½ MS medium along with 20 μM IBA for 24 h and encapsulation was carried thereafter. Combination of 3% sodium alginate augmented with 100 mM CaCl2.2H2O was found appropriate for the formation of clear and uniform beads and subsequent conversion of encapsulated nodal segments into plantlets. Maximum (66%) encapsulated nodal segments were converted into plantlets on MS medium supplemented with 7.5 μM BA and 0.5 μM NAA after eight weeks. Regeneration frequency of auxin-pretreated encapsulated and non-encapsulated nodal segments (stored at 4 oC) was evaluated at different storage time (0 to 6 weeks). After four weeks of storage, encapsulated propagules ex...
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nutrient alginate encapsulation of nodal segments of althaea officinalis l for short term conservation and germplasm exchange
Plant Biosystems, 2018Co-Authors: Ruphi Naz, Mohammad Anis, Abdulrahman A Alatar, Altaf Ahmad, Afshan NaazAbstract:In the present study, an alternate method for germplasm storage in the form of Artificial Seeds was standardized via nodal explants excised from in vitro proliferated shoots. The explants were enca...
Afshan Naaz - One of the best experts on this subject based on the ideXlab platform.
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nutrient alginate encapsulation of nodal segments of althaea officinalis l for short term conservation and germplasm exchange
Plant Biosystems, 2018Co-Authors: Mohammad Anis, Abdulrahman A Alatar, Altaf Ahmad, Afshan NaazAbstract:AbstractIn the present study, an alternate method for germplasm storage in the form of Artificial Seeds was standardized via nodal explants excised from in vitro proliferated shoots. The explants were encapsulated using sodium alginate and calcium chloride as gelling matrix. For development of root along with shoot, excised nodal segments were pretreated with ½ MS medium along with 20 μM IBA for 24 h and encapsulation was carried thereafter. Combination of 3% sodium alginate augmented with 100 mM CaCl2.2H2O was found appropriate for the formation of clear and uniform beads and subsequent conversion of encapsulated nodal segments into plantlets. Maximum (66%) encapsulated nodal segments were converted into plantlets on MS medium supplemented with 7.5 μM BA and 0.5 μM NAA after eight weeks. Regeneration frequency of auxin-pretreated encapsulated and non-encapsulated nodal segments (stored at 4 oC) was evaluated at different storage time (0 to 6 weeks). After four weeks of storage, encapsulated propagules ex...
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nutrient alginate encapsulation of nodal segments of althaea officinalis l for short term conservation and germplasm exchange
Plant Biosystems, 2018Co-Authors: Ruphi Naz, Mohammad Anis, Abdulrahman A Alatar, Altaf Ahmad, Afshan NaazAbstract:In the present study, an alternate method for germplasm storage in the form of Artificial Seeds was standardized via nodal explants excised from in vitro proliferated shoots. The explants were enca...
Christian Möllers - One of the best experts on this subject based on the ideXlab platform.
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Towards Artificial Seeds from microspore derived embryos of Brassica napus
Plant Cell Tissue and Organ Culture (PCTOC), 2019Co-Authors: Mohammed Cassim Mohammed Iqbal, Christian MöllersAbstract:Artificial Seeds from microspore derived embryos enhance breeding of Brassica species. Microspore derived embryos are haploid and their immediate diploidization generates doubled haploid homozygous plants, whereas a normal breeding process would take 7–8 generations to attain homozygosity. However, the flexibility available in conventional Seeds—storage, transport, variable planting time, and handling—is not possible with microspore derived embryos in breeding programs; they are continuously growing from induction of embryogenesis to planting in the soil, without a pause. Artificial seed technology can by-pass the expensive and time-consuming process of acclimatizing and loss of in vitro derived embryos in the green house. The doubled haploid technology in Brassica species has advanced considerably on many fronts—reliable induction of embryogenesis, in vitro diploidization, desiccation and conversion of embryos to plantlets. Although microspore derived embryos are bipolar, they are not considered within the scheme of Artificial Seeds. The development of Artificial Seeds in brassica, however, needs the input from other biotechnologies to develop vigorous embryos capable of conversion to plants. It is now necessary for empirical research on microspore derived embryos of Brassica to expand the applications available to the plant breeder by including the biotechnologies of encapsulation and embryo priming to develop Artificial Seeds. The objective of this review is to draw the attention of researchers to make the transition from microspore-derived embryos to Artificial Seeds in Brassica crop species, by bringing together relevant studies from the relevant biotechnologies. This would expand the present scope of Artificial Seeds and thus provide more options and flexibility to the Brassica plant breeder.
