The Experts below are selected from a list of 12738 Experts worldwide ranked by ideXlab platform
Yoshito Ikada - One of the best experts on this subject based on the ideXlab platform.
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Re-freeze dried bilayer Artificial Skin.
Biomaterials, 1993Co-Authors: Matsuda Kazuya, Shigehiko Suzuki, Nobuhiko Isshiki, Yoshito IkadaAbstract:A bilayer Artificial Skin composed of a silicone sheet and a collagen sponge sheet was developed by us in 1986, modifying Yannas and Burke's technique. It was used in experimental animals and clinically, both with success; but the Artificial Skin was inconvenient for clinical use in terms of disinfection and preservation. In an attempt to reduce the drawbacks, we developed a re-freeze dried Artificial Skin. Experiments were conducted to examine whether the re-freeze dried material is equivalent to the prototype one. A significant difference between the two was found in tensile tests but not in experimental and clinical effects.
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Evaluation of a bilayer Artificial Skin capable of sustained release of an antibiotic.
Biomaterials, 1992Co-Authors: Matsuda Kazuya, Shigehiko Suzuki, Nobuhiko Isshiki, Kazuo Yoshioka, Ryoichi Wada, S.-h. Hyon, Yoshito IkadaAbstract:A bilayer Artificial Skin, composed of an upper silicone sheet and a lower collagen sponge, has been developed by modifying a technique proposed by Yannas and Burke. We have applied it clinically with success, but infection sometimes occurred in the area where the Artificial Skin was placed. To use it safely in an infected wound, we developed a new type of Artificial Skin capable of sustained release of antibiotic. Microspheres of poly-l-lactic acid containing an antibiotic, were installed in the upper silicone sheet. The usefulness of the new type of Artificial Skin was suggested by in vitro studies.
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A bilayer "Artificial Skin" capable of sustained release of an antibiotic.
British journal of plastic surgery, 1991Co-Authors: Matsuda Kazuya, Shigehiko Suzuki, Nobuhiko Isshiki, Kazuo Yoshioka, S.-h. Hyon, Toshiyuki Okada, Yoshito IkadaAbstract:Abstract The most frequent complication of the bilayer "Artificial Skin", composed of a silicone sheet and collagen sponge described in a previous paper, was infection beneath it. This paper describes a new type of "Artificial Skin" in which microspheres containing antibiotics were installed beneath the silicone sheet, allowing a continuous release of antibiotics.
Adalberto Vieira Corazza - One of the best experts on this subject based on the ideXlab platform.
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The effect of combined curcumin-mediated photodynamic therapy and Artificial Skin on Staphylococcus aureus–infected wounds in rats
Lasers in Medical Science, 2020Co-Authors: Fernanda Rossi Paolillo, Phamilla Gracielli Sousa Rodrigues, Vanderlei Salvador Bagnato, Fernanda Alves, Layla Pires, Adalberto Vieira CorazzaAbstract:Healing wounds represent a major public health problem, mainly when it is infected. Besides that, the antibiotics misuse and overuse favor the development of bacterial resistance. This study evaluated the effects of antimicrobial photodynamic therapy (aPDT) combined with Artificial Skin on disinfection of infected Skin wound in rats. Twenty-four Wistar rats were randomly distributed into 4 groups ( n = 6): (i) control—untreated; (ii) aPDT—treated with curcumin-mediated aPDT (blue light); (iii) Artificial Skin—treated with Artificial Skin alcohol-based; and (iv) aPDT plus Artificial Skin—treated with aPDT associated with Artificial Skin alcohol-based. For the in vivo model, a full-thickness biopsy with 0.80 cm was performed in order to inoculate the microorganism Staphylococcus aureus (ATCC 25923). The aPDT was performed with a curcumin gel and a blue LED light (450 nm, 80 mW/cm^2) at the dose of 60 J/cm^2 and the treatment with alcohol-based Artificial Skin was done with the topical application of 250 μL. Additional animals were submitted to aPDT combined with the Artificial Skin. After treatments, the number of colony-forming units (CFU) and the damage area were determined. Data were analyzed by two-way repeated measures ANOVA and Tukey tests. The highest reduction of the bacterial viability was observed in the PDT plus Artificial Skin group (4.14 log10), followed by Artificial Skin (2.38 log10) and PDT (2.22 log10) groups. In addition, all treated groups showed higher relative area of wound contraction (36.21% for the PDT, 38.41% for Artificial Skin, and 35.02% for PDT plus Artificial) in comparison with the control group. These findings provide evidence for the positive benefits of aPDT with blue light and curcumin associated with Artificial Skin to decontaminate and accelerate the wound contraction.
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The effect of combined curcumin-mediated photodynamic therapy and Artificial Skin on Staphylococcus aureus-infected wounds in rats.
Lasers in medical science, 2020Co-Authors: Fernanda Rossi Paolillo, Phamilla Gracielli Sousa Rodrigues, Vanderlei Salvador Bagnato, Fernanda Alves, Layla Pires, Adalberto Vieira CorazzaAbstract:Healing wounds represent a major public health problem, mainly when it is infected. Besides that, the antibiotics misuse and overuse favor the development of bacterial resistance. This study evaluated the effects of antimicrobial photodynamic therapy (aPDT) combined with Artificial Skin on disinfection of infected Skin wound in rats. Twenty-four Wistar rats were randomly distributed into 4 groups (n = 6): (i) control-untreated; (ii) aPDT-treated with curcumin-mediated aPDT (blue light); (iii) Artificial Skin-treated with Artificial Skin alcohol-based; and (iv) aPDT plus Artificial Skin-treated with aPDT associated with Artificial Skin alcohol-based. For the in vivo model, a full-thickness biopsy with 0.80 cm was performed in order to inoculate the microorganism Staphylococcus aureus (ATCC 25923). The aPDT was performed with a curcumin gel and a blue LED light (450 nm, 80 mW/cm2) at the dose of 60 J/cm2 and the treatment with alcohol-based Artificial Skin was done with the topical application of 250 μL. Additional animals were submitted to aPDT combined with the Artificial Skin. After treatments, the number of colony-forming units (CFU) and the damage area were determined. Data were analyzed by two-way repeated measures ANOVA and Tukey tests. The highest reduction of the bacterial viability was observed in the PDT plus Artificial Skin group (4.14 log10), followed by Artificial Skin (2.38 log10) and PDT (2.22 log10) groups. In addition, all treated groups showed higher relative area of wound contraction (36.21% for the PDT, 38.41% for Artificial Skin, and 35.02% for PDT plus Artificial) in comparison with the control group. These findings provide evidence for the positive benefits of aPDT with blue light and curcumin associated with Artificial Skin to decontaminate and accelerate the wound contraction.
Jung Keug Park - One of the best experts on this subject based on the ideXlab platform.
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Tissue engineered Artificial Skin composed of dermis and epidermis.
Artificial organs, 2000Co-Authors: Eun-kyung Yang, Young Kwon Seo, Hee Hun Youn, Doo Hoon Lee, Sue Nie Park, Jung Keug ParkAbstract:: We made an Artificial Skin comprised of a stratified layer of keratinocytes and a dermal matrix with a type I collagen containing fibroblasts. In this work, we showed keratinocyte behavior under primary culture, gel contractions varying with concentration of collagen solution, and cell growth plots in the collagen gel. The optimum behavior of dermal equivalent could be obtained using 3.0 mg/ml collagen solution and attached gel culture. The attached gel culture had a jumping effect of growth factor on cell growth at the lag phase. To develop the Artificial Skin, 1× 105 cells/cm2 of keratinocytes were cultured on the dermal equivalent at air-liquid interface. Finally, to overcome the problem that Artificial Skin of collagen gel was torn easily during suturing of grafting, we prepared histocompatible collagen mesh and attached the mesh to the bottom of the gel. Cultured Artificial Skins were successfully grafted onto rats.
Matsuda Kazuya - One of the best experts on this subject based on the ideXlab platform.
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Further applications of “bilayer Artificial Skin”
British journal of plastic surgery, 1995Co-Authors: Shigehiko Suzuki, Matsuda Kazuya, T. Maruguchi, Yoshihiko Nishimura, Y. IkadaAbstract:Abstract A "bilayer Artificial Skin", composed of an inner layer of collagen sponge and an outer silicone layer, was developed by modifying the material reported by Yannas and Burke. Since our early results from experimental and clinical use of the original version of the "bilayer Artificial Skin" were reported, several improvements have been made in stages to eliminate some drawbacks related to disinfection and preservation and to reduce the primary cost of manufacture. The latest version of the material was successfully used in 27 sites on 23 patients. In this paper, the improvements in the material and the clinical results are described.
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Re-freeze dried bilayer Artificial Skin.
Biomaterials, 1993Co-Authors: Matsuda Kazuya, Shigehiko Suzuki, Nobuhiko Isshiki, Yoshito IkadaAbstract:A bilayer Artificial Skin composed of a silicone sheet and a collagen sponge sheet was developed by us in 1986, modifying Yannas and Burke's technique. It was used in experimental animals and clinically, both with success; but the Artificial Skin was inconvenient for clinical use in terms of disinfection and preservation. In an attempt to reduce the drawbacks, we developed a re-freeze dried Artificial Skin. Experiments were conducted to examine whether the re-freeze dried material is equivalent to the prototype one. A significant difference between the two was found in tensile tests but not in experimental and clinical effects.
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Evaluation of a bilayer Artificial Skin capable of sustained release of an antibiotic.
Biomaterials, 1992Co-Authors: Matsuda Kazuya, Shigehiko Suzuki, Nobuhiko Isshiki, Kazuo Yoshioka, Ryoichi Wada, S.-h. Hyon, Yoshito IkadaAbstract:A bilayer Artificial Skin, composed of an upper silicone sheet and a lower collagen sponge, has been developed by modifying a technique proposed by Yannas and Burke. We have applied it clinically with success, but infection sometimes occurred in the area where the Artificial Skin was placed. To use it safely in an infected wound, we developed a new type of Artificial Skin capable of sustained release of antibiotic. Microspheres of poly-l-lactic acid containing an antibiotic, were installed in the upper silicone sheet. The usefulness of the new type of Artificial Skin was suggested by in vitro studies.
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A bilayer "Artificial Skin" capable of sustained release of an antibiotic.
British journal of plastic surgery, 1991Co-Authors: Matsuda Kazuya, Shigehiko Suzuki, Nobuhiko Isshiki, Kazuo Yoshioka, S.-h. Hyon, Toshiyuki Okada, Yoshito IkadaAbstract:Abstract The most frequent complication of the bilayer "Artificial Skin", composed of a silicone sheet and collagen sponge described in a previous paper, was infection beneath it. This paper describes a new type of "Artificial Skin" in which microspheres containing antibiotics were installed beneath the silicone sheet, allowing a continuous release of antibiotics.
M W Strohmayr - One of the best experts on this subject based on the ideXlab platform.
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the dlr Artificial Skin step i uniting sensitivity and collision tolerance
International Conference on Robotics and Automation, 2013Co-Authors: M W Strohmayr, Heinz Worn, G HirzingerAbstract:The integration of Artificial Skin into robotic systems has long been foreseen. For the last decades the introduction was always just a couple of years away. One of the possible reasons for the slow progress is the focus of the research efforts on high sensitivity and spatial resolution. The resulting tactile sensor prototypes are presented as laboratory prototypes with no or little chance to be successfully integrated into a robotic system. In order to enable the operation of an Artificial Skin on a robotic system in real-world applications the conflict of goals between high sensitivity and an overload-proof, collision tolerant, design needs to be solved. Within this paper we present a stretchable tactile surface sensor as a major functional component of an Artificial Skin setup that is able to unite the required sensitivity with the ability to withstand collisions. The results of first experiments regarding sensitivity and the ability of the DLR Artificial Skin setup to withstand high impact forces are presented.
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ICRA - The DLR Artificial Skin step I: Uniting sensitivity and collision tolerance
2013 IEEE International Conference on Robotics and Automation, 2013Co-Authors: M W Strohmayr, Heinz Worn, G HirzingerAbstract:The integration of Artificial Skin into robotic systems has long been foreseen. For the last decades the introduction was always just a couple of years away. One of the possible reasons for the slow progress is the focus of the research efforts on high sensitivity and spatial resolution. The resulting tactile sensor prototypes are presented as laboratory prototypes with no or little chance to be successfully integrated into a robotic system. In order to enable the operation of an Artificial Skin on a robotic system in real-world applications the conflict of goals between high sensitivity and an overload-proof, collision tolerant, design needs to be solved. Within this paper we present a stretchable tactile surface sensor as a major functional component of an Artificial Skin setup that is able to unite the required sensitivity with the ability to withstand collisions. The results of first experiments regarding sensitivity and the ability of the DLR Artificial Skin setup to withstand high impact forces are presented.
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IROS - The DLR Artificial Skin step II: Scalability as a prerequisite for whole-body covers
2013 IEEE RSJ International Conference on Intelligent Robots and Systems, 2013Co-Authors: M W Strohmayr, D. SchneiderAbstract:In human Skin, the ability to spatially discriminate an individual indentation from two simultaneous indentations is tailored to the need of the specific area of application on the human body. While the spatial resolution is comparatively low over wide areas of the human body, there are no insensitive spots. In addition, the measuring range is tuned to the expected loads on the respective part of the human body. Within this study these observations are utilized to solve some of the key challenges on the way towards an Artificial Skin as a whole-body cover for robotic systems. To enable the reliable detection of collision events which are commonly of very short duration the reaction time of the Artificial Skin system has to be minimized. In order to do so, the goal conflict between the required number of taxels and the required high readout frequencies has to be solved. We present the DLR approach towards scalable transduction hardware and readout electronics as a basis for the acquisition of tactile information from future whole-body covers. First experiments with prototypes of the DLR Artificial Skin demonstrate the scalability of the transduction hardware with respect to size, spatial resolution and measuring range.
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Artificial Skin in Robotics --A Comprehensive Interface for System-Environment Interaction
2012Co-Authors: M W StrohmayrAbstract:Modern robotic systems are gradually escaping their fenced workspace and begin to physically interact with humans. If the physical barriers between the workspace of the robotic system and the human fall away, new safety and interaction concepts for the physical human robot interaction have to be developed. These concepts have to make sure, that, e.g. during a joint assembly task within a car body neither the human nor the interacting robotic system are endangered. Sensor systems are a key element to detect changes in the environment and sense actions of the human user. One aspect herein is the detection of direct physical contact between the robotic system and the environment or the human user. In order to ensure safety of human and robotic system a close surveillance of the covering structure of the robotic system with respect to physical contact is desired. A promising solution are spatially distributed tactile sensors that are mounted on the covering structure of the robotic system. The acquired tactile information can be utilized to initiate adequate collision reaction strategies or as a means of intuitive human machine communication. A major challenge for the development of tactile sensors for robotic systems is the adaptation to the complex, often 3D-curved surfaces of modern robotic systems. This adaptation is not or only insufficiently possible with the available tactile sensors. The analysis of the state-of-the-art reveals, that the applied materials and manufacturing technologies restricts the majority of tactile sensors to the application on planar or developable surfaces. If robotic systems operate in an unstructured and time varying environment collisions can no longer be avoided. That is why, amongst other means, the surface of the covering structure of the robotic system has to be equipped with a passive mechanical damping layer. In order not to constrain the sensitivity of the tactile sensors, the tactile sensors have to be integrated on top of the mechanical damping layer. Consequently, the tactile surface sensors have to be stretchable to allow for the underlying mechanical damping layer to deform in case of a collision. In addition, the tactile surface sensors have to be overload proof to withstand the high indentation forces that occur in case of a collision. In the past, the majority of approaches towards tactile sensors for robotic systems focussed on high spatial resolution and sensitivity. A future integration into a robotic system or the mechanical robustness of the tactile sensors have often been neglected. However, if the development was focussed on mechanical robustness, the resulting tactile sensors lack the required sensitivity. The analysis of the current state-of-the-art of science and technology impressively shows that, considered individually, all requirements can be fulfilled – but not their combination. Therefore the focus of this thesis is the derivation of a solution of the goal conflict between the desired high sensitivity and the required mechanical robustness. Human Skin is considered as a design metaphor for the development of a multi functional Artificial Skin concept that, next to providing the required sensory capabilities, exhibits mechanical deformation and damping properties required for the operation on a robotic system. Based on the analysis of the current state-of-the-art of science and technology and the outcome of own previous work design paradigms for the solution of the described goal conflict are proposed. The development and the structure of this thesis are based on the design methodology for mechatronic systems presented in VDI 2206. Herein the V-model, known from software engineering, is adapted for the development of mechatronic systems. During the system design an overall concept for a scalable Artificial Skin is derived. The concept allows the adaptation of the properties of the Artificial Skin to the respective application site on the robotic system. Besides the scalability of sensor surface area, spatial resolution and sensitivity the pursued approach accounts for scalability of the underlying manufacturing processes that is required for the successful integration into robotic systems. In addition, a concept for the acquisition and preprocessing of the tactile data is proposed. Based on the functional partitioning, known from software design, the desired functional range of an Artificial Skin is divided in adjustable functional components. During the domain specific design concepts for the individual functional components are derived based on the integrative design of tailored materials and scalable manufacturing processes. As an example, novel electrically conductive polymer based circuit tracks are developed in order to enable the required elastic deformability of the sensitive surface area of the Artificial Skin. The properties of a future Artificial Artificial Skin system can be anticipated based on the conducted FEM simulation. Currently no standardized test procedure for the assessment of the functionality of tactile sensor exists, therefore a simplified test procedure for the verification of the desired properties of the Artificial Skin is proposed. Within the system integration exemplarily a tactile surface sensor for the acquisition of normal indentation forces is implemented. For this, the required functional components are combined and the underlying manufacturing processes are field-tested. The resulting Artificial Skin prototypes are identified on a specialized testbed and applied on a robotic system. The suitability for daily use of the Artificial Skin prototypes is examined in a collision detection scenario on the DLR LWR III. The conducted tests demonstrate, that the proposed overall design concept allows for the development of an Artificial Skin that is scalable with respect to sensor surface area, spatial resolution and sensitivity. The prototypes and the conducted experiments verify that the presented Artificial Skin can be operated on 3D-curved surfaces of modern robotic systems and that the goal conflict between sensitivity and a collision tolerant design can be solved.
