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Wageh Sobhy Darwish - One of the best experts on this subject based on the ideXlab platform.

  • identification of lead produced lipid hydroperoxides in human hepg2 cells and protection using rosmarinic and Ascorbic Acids with a reference to their regulatory roles on nrf2 keap1 antioxidant pathway
    Chemico-Biological Interactions, 2019
    Co-Authors: Yonghan Li, Wageh Sobhy Darwish, Zhen Chen, Yue Wu, Suzuki Hirotaka, Hitoshi Chiba
    Abstract:

    Abstract Lead (Pb) is one of the toxic heavy metals that have several toxicological implications including cytotoxicities and oxidative stress. The release of reactive oxygen species (ROS) usually initiates lipid peroxidation and resulting in inflammation and tissue injury. However, the detailed identification of the Pb-produced lipid hydroperoxides has received little attention. Furthermore, the mechanisms behind such effects are less informed. Therefore, this study firstly investigated Pb-produced lipid hydroperoxides in human HepG2 cells using LC/MS. The effects of Pb on the antioxidant enzymes were additionally examined using qPCR and their dependent activities. As a protection trial, the ameliorative effects of rosmarinic (RMA) and Ascorbic (ASA) Acids on Pb-induced cytotoxicity and oxidative stress and their regulatory effects on Nrf2/Keap1 pathway were investigated. The achieved results confirmed cytotoxicity and oxidative damage of Pb on HepG2 cells. In addition, 20 lipid hydroperoxides (LOOH) were identified including 11 phosphatidylcholine hydroperoxides (PCOOH), 5 triacylglycerol hydroperoxides (TGOOH) and 4 cholesteryl ester hydroperoxides (CEOOH). The most dominant LOOH species were PCOOH 34:2, PCOOH 34:3, PCOOH 38:7, TGOOH 60:14, TGOOH 60:15, CEOOH 18:3 and CEOOH 20:4. Pb significantly downregulated Nrf2-regulated antioxidant enzymes at both the pretranscriptional and functional levels. Co-exposure of HepG2 cells to RMA and ASA significantly reduced Pb-produced adverse outcomes. This protection occurred via activation Nrf2-Keap1 antioxidant pathway.

  • determination of polycyclic aromatic hydrocarbon content in heat treated meat retailed in egypt health risk assessment benzo a pyrene induced mutagenicity and oxidative stress in human colon caco 2 cells and protection using rosmarinic and Ascorbic a
    Food Chemistry, 2019
    Co-Authors: Wageh Sobhy Darwish, Hitoshi Chiba, Abdelazim Elsayed Elhelaly, Waleed R Elghareeb
    Abstract:

    Abstract This study was undertaken to estimate the concentrations of the formed polycyclic aromatic hydrocarbons (PAHs) in heat-treated (boiled, pan-fried and grilled) meats collected from Egypt. Dietary intakes and cancer risks of PAHs among Egyptian adults were calculated. Benzo[a]pyrene (B[a]P)-induced mutagenicity and oxidative stress in human colon (CaCo-2) cell line and mechanisms behind such effects were also investigated. Finally, protection trials using rosmarinic (RMA) and Ascorbic Acids (ASA) were carried out. The results indicated formation of PAHs at high levels in the heat-treated meats. Calculated incremental life time cancer risk among Egyptian adults were 7.05179E−07, 7.00604 E−06 and 1.86069 E−05 due to ingestion of boiled, pan-fried and grilled meats, respectively. B[a]P-exposed CaCo-2 cells had high abilities for mutagenicity (490.05 ± 21.37 His + revertants) and production of reactive oxygen species. RMA and ASA protected CaCo-2 cells via reduction of B[a]P-induced mutagenicity and oxidative stress and upregulation of phase II detoxification enzymes and xenobiotic transporters.

  • estimation of cadmium content in egyptian foodstuffs health risk assessment biological responses of human hepg2 cells to food relevant concentrations of cadmium and protection trials using rosmarinic and Ascorbic Acids
    Environmental Science and Pollution Research, 2019
    Co-Authors: Wageh Sobhy Darwish, Hitoshi Chiba, Abdelazim Elsayed Elhelaly
    Abstract:

    Cadmium (Cd) is an environmental pollutant that can get entry into human body via ingestion of contaminated foods causing multiple organ damage. This study aimed at monitoring Cd residues in 20 foodstuffs of animal origin that are commonly consumed in Egypt. Health risk assessment was conducted via calculation of Cd dietary intakes and non-carcinogenic target hazard quotient. An in vitro approach was performed to investigate the constitutive effects of Cd on human hepatoma (HepG2) cells under food-relevant concentrations. Trials to reduce Cd-induced adverse effects on HepG2 cells were done using rosmarinic (RMA) and Ascorbic Acids (ASA). The achieved results indicated contamination of the tested foodstuffs with Cd at high levels with potential human health hazards. Cd at food-relevant concentrations caused significant cytotoxicity to HepG2 cells. This may be attributed to induction of oxidative stress and inflammation, as indicated by the overexpression of stress and inflammatory markers. At the same time, Cd downregulated xenobiotic transporters and upregulated the proliferation factors. Co-exposure of HepG2 cells to Cd and micronutrients such as RMA and ASA led to recovery of cells from the oxidative damage, and subsequently cell viability was strongly improved. RMA and ASA ameliorated the biological responses of HepG2 cells to Cd exposure.

Alfredo Montano - One of the best experts on this subject based on the ideXlab platform.

  • fermented vegetables containing benzoic and Ascorbic Acids as additives benzene formation during storage and impact of additives on quality parameters
    Journal of Agricultural and Food Chemistry, 2011
    Co-Authors: Francisco Javier Casado, Antonio Higinio Sanchez, Victor Manuel Beato, Antonio De Castro, Luis Rejano, Alfredo Montano
    Abstract:

    Chemical and sensorial changes related to the use of benzoates and Ascorbic acid as additives in packed fermented vegetables were investigated. For this, three selected vegetables (green olives, cucumbers, and caperberries) stored under different conditions (glass or plastic containers, ambient or refrigerated storage) were used. In all cases, benzoic acid remained unchanged (glass bottle) or decreased slightly (plastic pouch) at prolonged storage. Ascorbic acid was partially or totally degraded during storage, the degradation rate depending on the storage conditions and the vegetable matrix. Benzene levels higher than 10 μg/L were found in cucumbers and caperberries containing both additives, but only when packed in plastic pouches and after prolonged storage at room temperature. In these conditions, an appreciable browning of brine, related to AA degradation, was also found. The use of benzoate alone had a significant influence on vegetable color, but flavor was not significantly affected at the benzoat...

  • Fermented vegetables containing benzoic and Ascorbic Acids as additives: benzene formation during storage and impact of additives on quality parameters.
    Journal of Agricultural and Food Chemistry, 2011
    Co-Authors: Francisco Javier Casado, Antonio Higinio Sanchez, Victor Manuel Beato, Antonio De Castro, Luis Rejano, Alfredo Montano
    Abstract:

    Chemical and sensorial changes related to the use of benzoates and Ascorbic acid as additives in packed fermented vegetables were investigated. For this, three selected vegetables (green olives, cucumbers, and caperberries) stored under different conditions (glass or plastic containers, ambient or refrigerated storage) were used. In all cases, benzoic acid remained unchanged (glass bottle) or decreased slightly (plastic pouch) at prolonged storage. Ascorbic acid was partially or totally degraded during storage, the degradation rate depending on the storage conditions and the vegetable matrix. Benzene levels higher than 10 μg/L were found in cucumbers and caperberries containing both additives, but only when packed in plastic pouches and after prolonged storage at room temperature. In these conditions, an appreciable browning of brine, related to AA degradation, was also found. The use of benzoate alone had a significant influence on vegetable color, but flavor was not significantly affected at the benzoate levels tested. On the basis of the present study, benzoates should be removed from fermented vegetable formulations containing Ascorbic acid to eliminate possible benzene formation during long-term storage.

  • fermented vegetables containing benzoic and Ascorbic Acids as additives benzene formation during storage and impact of additives on quality parameters
    Journal of Agricultural and Food Chemistry, 2011
    Co-Authors: Francisco Javier Casado, Antonio Higinio Sanchez, Victor Manuel Beato, Antonio De Castro, Luis Rejano, Alfredo Montano
    Abstract:

    Chemical and sensorial changes related to the use of benzoates and Ascorbic acid as additives in packed fermented vegetables were investigated. For this, three selected vegetables (green olives, cu...

  • stability of sorbic and Ascorbic Acids in packed green table olives during long term storage as affected by different packing conditions and its influence on quality parameters
    Food Chemistry, 2010
    Co-Authors: Francisco Javier Casado, Antonio Higinio Sanchez, Antonio De Castro, Luis Rejano, Alfredo Montano
    Abstract:

    Abstract The effect of different packing conditions on sorbate stability was studied in Spanish-type green table olives stored for up to one year. The factors studied were preservation treatment (pasteurisation vs. non-pasteurisation), storage temperature (room temperature vs refrigeration), packaging material (glass bottle vs. plastic pouch), storage time, and the presence or not of an antioxidant additive (Ascorbic acid). Sorbic acid was stable in pasteurised samples as well as in the refrigerated unpasteurised samples, both in the absence and in the presence of Ascorbic acid. In unpasteurised samples stored at room temperature, sorbic acid gradually disappeared, with a concomitant increase in the concentration of trans -4-hexenoic acid, which could be attributable to the action of lactic acid bacteria. Ascorbic acid was always less stable than sorbate. Sorbate addition negatively affected the colour parameters of both fruit and brine. An appreciable off-flavour was detected in the samples in which sorbate had been degraded.

Abdelazim Elsayed Elhelaly - One of the best experts on this subject based on the ideXlab platform.

  • determination of polycyclic aromatic hydrocarbon content in heat treated meat retailed in egypt health risk assessment benzo a pyrene induced mutagenicity and oxidative stress in human colon caco 2 cells and protection using rosmarinic and Ascorbic a
    Food Chemistry, 2019
    Co-Authors: Wageh Sobhy Darwish, Hitoshi Chiba, Abdelazim Elsayed Elhelaly, Waleed R Elghareeb
    Abstract:

    Abstract This study was undertaken to estimate the concentrations of the formed polycyclic aromatic hydrocarbons (PAHs) in heat-treated (boiled, pan-fried and grilled) meats collected from Egypt. Dietary intakes and cancer risks of PAHs among Egyptian adults were calculated. Benzo[a]pyrene (B[a]P)-induced mutagenicity and oxidative stress in human colon (CaCo-2) cell line and mechanisms behind such effects were also investigated. Finally, protection trials using rosmarinic (RMA) and Ascorbic Acids (ASA) were carried out. The results indicated formation of PAHs at high levels in the heat-treated meats. Calculated incremental life time cancer risk among Egyptian adults were 7.05179E−07, 7.00604 E−06 and 1.86069 E−05 due to ingestion of boiled, pan-fried and grilled meats, respectively. B[a]P-exposed CaCo-2 cells had high abilities for mutagenicity (490.05 ± 21.37 His + revertants) and production of reactive oxygen species. RMA and ASA protected CaCo-2 cells via reduction of B[a]P-induced mutagenicity and oxidative stress and upregulation of phase II detoxification enzymes and xenobiotic transporters.

  • estimation of cadmium content in egyptian foodstuffs health risk assessment biological responses of human hepg2 cells to food relevant concentrations of cadmium and protection trials using rosmarinic and Ascorbic Acids
    Environmental Science and Pollution Research, 2019
    Co-Authors: Wageh Sobhy Darwish, Hitoshi Chiba, Abdelazim Elsayed Elhelaly
    Abstract:

    Cadmium (Cd) is an environmental pollutant that can get entry into human body via ingestion of contaminated foods causing multiple organ damage. This study aimed at monitoring Cd residues in 20 foodstuffs of animal origin that are commonly consumed in Egypt. Health risk assessment was conducted via calculation of Cd dietary intakes and non-carcinogenic target hazard quotient. An in vitro approach was performed to investigate the constitutive effects of Cd on human hepatoma (HepG2) cells under food-relevant concentrations. Trials to reduce Cd-induced adverse effects on HepG2 cells were done using rosmarinic (RMA) and Ascorbic Acids (ASA). The achieved results indicated contamination of the tested foodstuffs with Cd at high levels with potential human health hazards. Cd at food-relevant concentrations caused significant cytotoxicity to HepG2 cells. This may be attributed to induction of oxidative stress and inflammation, as indicated by the overexpression of stress and inflammatory markers. At the same time, Cd downregulated xenobiotic transporters and upregulated the proliferation factors. Co-exposure of HepG2 cells to Cd and micronutrients such as RMA and ASA led to recovery of cells from the oxidative damage, and subsequently cell viability was strongly improved. RMA and ASA ameliorated the biological responses of HepG2 cells to Cd exposure.

Roberto Ballini - One of the best experts on this subject based on the ideXlab platform.

  • inhibition of microbiological activity during sole solea solea l chilled storage by applying ellagic and Ascorbic Acids
    Lwt - Food Science and Technology, 2008
    Co-Authors: B Zambuchini, Dennis Fiorini, Maria Cristina Verdenelli, C Orpianesi, Roberto Ballini
    Abstract:

    Abstract Fresh fish products are commonly sold at storage temperature. However, many undesirable changes can occur in the products during storage, due to microbial growth and fish spoilage, affording evident economic loss. The effects of ellagic acid (EA) and l -Ascorbic acid ( l -AA)/sodium ascorbate (SA), either alone or in combination, on the microbiological quality of fresh finfish Solea solea during storage at 0 °C, were investigated. The results showed that addition of EA (0.03%) alone or in combination with l -AA (1.71%)/SA (1.98%) significantly delayed the proliferation of aerobic plate counts, psychrotrophic counts and Pseudomonas bacteria extending the product shelf life up to 10 days, versus 8 days for control. During the storage, the pH increase is slowed down when the fish samples are subjected to the treatment with preservative agents. The effect of lowering the pH increase is particularly pronounced when a combination of EA and l -AA/SA is used. Therefore, EA alone or in combination with l -AA and SA can be successfully utilized to reduce the microbial growth, extending the shelf life of fish during storage at 0 °C.

Itaru Yamamoto - One of the best experts on this subject based on the ideXlab platform.

  • permeation and metabolism of a series of novel lipophilic Ascorbic acid derivatives 6 o acyl 2 o α d glucopyranosyl l Ascorbic Acids with a branched acyl chain in a human living skin equivalent model
    Bioorganic & Medicinal Chemistry Letters, 2004
    Co-Authors: Satomi Goto, Yutaka Ishiguro, Kazuko Suzuki, Teruhiko Nitoda, Itaru Yamamoto
    Abstract:

    Abstract A series of novel lipophilic vitamin C derivatives, 6- O -acyl-2- O -α- d -glucopyranosyl- l -Ascorbic Acids possessing a branched-acyl chain of varying length from C 8 to C 16 (6-bAcyl-AA-2G), were evaluated as topical prodrugs of Ascorbic acid (AA) with transdermal activity in a human living skin equivalent model. The permeability of 6-bAcyl-AA-2G was compared with those of the derivatives having a straight-acyl chain (6-sAcyl-AA-2G). Out of 10 derivatives of 6-sAcyl-AA-2G and 6-bAcyl-AA-2G, 6-sDode-AA-2G and 6-bDode-AA-2G exhibited most excellent permeability in this model. Measurement of the metabolites permeated from the skin model suggested that 6-bDode-AA-2G was mainly hydrolyzed via 6- O -acyl AA to AA by tissue enzymes, while 6-sDode-AA-2G was hydrolyzed via 2- O -α- d -glucopyranosyl- l -Ascorbic acid to AA. The former metabolic pathway seems to be advantageous for a readily available source of AA, because 6- O -acyl AA, as well as AA, is able to show vitamin C activity.

  • synthesis and characterization of 6 o acyl 2 o α d glucopyranosyl l Ascorbic Acids with a branched acyl chain
    Chemical & Pharmaceutical Bulletin, 2003
    Co-Authors: Daisuke Kawasaki, Kenji Sasaki, Eiichi Gohda, Itaru Yamamoto
    Abstract:

    : We previously reported the chemical synthesis of a series of novel monoacylated vitamin C derivatives, 6-O-acyl-2-O-alpha-D-glucopyranosyl-L-Ascorbic Acids (6-Acyl-AA-2G) possessing a straight-acyl chain of varying length from C(4) to C(18), as effective skin antioxidants. In this paper, we describe branched type of 6-Acyl-AA-2G derivatives (6-bAcyl-AA-2G) synthesized by use of a 2-branched-chain fatty acid anhydride as an acyl donor. The stability of 6-bAcyl-AA-2G in neutral solution was much higher than that of 6-Acyl-AA-2G, while they were susceptible to enzymatic hydrolysis for exerting vitamin C effect. These branched derivatives as well as 6-Acyl-AA-2G increased the radical scavenging activity against 1, 1-diphenyl-2-picrylhydrazyl and the lipophilicity in octanol/water-partitioning systems with increasing length of their acyl group. In addition, the 6-bAcyl-AA-2G derivative with an acyl chain of C(12), 6-bDode-AA-2G had the excellent solubility to various solvents, suggesting easy handling in cosmetic use. These characteristics of 6-bAcyl-AA-2G may be available for skin care application as an effective antioxidant.

  • bioavailability of a series of novel acylated Ascorbic acid derivatives 6 o acyl 2 o α d glucopyranosyl l Ascorbic Acids as an Ascorbic acid
    Bioscience Biotechnology and Biochemistry, 2002
    Co-Authors: Yoshihito Fujinami, Kyoko Matsumoto, Daisuke Kawasaki, Itaru Yamamoto
    Abstract:

    The bioavailability of a series of novel acylated Ascorbic acid derivatives, 6-O-acyl-2-O-α-D-glucopyranosyl-L-Ascorbic Acids (6-Acyl-AA-2G), as an Ascorbic acid (AA) supplement was investigated in rats and guinea pigs. Oral administration of 6-Acyl-AA-2G to rats resulted in an increase in the plasma AA level. However, the intact form was not detectable in the plasma by high-performance liquid chromatography, indicating its hydrolysis through the process of absorption. After an intravenous injection to rats of 6-Octa-AA-2G as a representative derivative, the intact form rapidly disappeared from the plasma, being followed by a prolonged and marked elevation of the plasma AA level. Various tissue homogenates from guinea pigs were examined for their releasing activity of AA, 2-O-α-D-glucopyranosyl-L-Ascorbic acid (AA-2G) and 6-O-acyl-AA from 6-Acyl-AA-2G. High activity was observed in the small intestine. These hydrolytic activities to AA and 6-O-acyl-AA were completely inhibited by castanospermine, an α-glu...

  • synthesis and characterization of a series of novel monoacylated Ascorbic acid derivatives 6 o acyl 2 o α d glucopyranosyl l Ascorbic Acids as skin antioxidants
    Journal of Medicinal Chemistry, 2002
    Co-Authors: Itaru Yamamoto, Yoshihito Fujinami, Kenji Sasaki, Shino Okazaki
    Abstract:

    A series of novel monoacylated vitamin C derivatives were chemically synthesized with a stable ascorbate derivative, 2-O-α-d-glucopyranosyl-l-Ascorbic acid (AA-2G), and acid anhydrides in pyridine. Their solubility in organic phase, thermal stability, radical scavenging activity, and in vitro skin permeability was evaluated. These monoacylated derivatives were identified as 6-O-acyl-2-O-α-d-glucopyranosyl-l-Ascorbic Acids (6-Acyl-AA-2G) by UV spectra, elemental analyses, and nuclear magnetic resonance spectroscopy. The reactions afforded 6-Acyl-AA-2G in high yields (30−60%). 6-Acyl-AA-2G exhibited satisfactory stability in neutral solution comparable to that of a typical stable derivative, AA-2G, and also showed the radical scavenging activity. The lipid solubility of 6-Acyl-AA-2G was increased with increasing length of their acyl group. Increased skin permeability was superior to those of AA-2G and Ascorbic acid (AsA). 6-Acyl-AA-2G that is susceptible to enzymatic hydrolysis by tissue esterase and/or α-g...

  • Bioavailability of a series of novel acylated Ascorbic acid derivatives, 6-O-acyl-2-O-alpha-D-glucopyranosyl-L-Ascorbic Acids, as an Ascorbic acid supplement in rats and guinea pigs.
    Bioscience biotechnology and biochemistry, 2002
    Co-Authors: Akihiro Tai, Yoshihito Fujinami, Kyoko Matsumoto, Daisuke Kawasaki, Itaru Yamamoto
    Abstract:

    The bioavailability of a series of novel acylated Ascorbic acid derivatives, 6-O-acyl-2-O-alpha-D-glucopyranosyl-L-Ascorbic Acids (6-Acyl-AA-2G), as an Ascorbic acid (AA) supplement was investigated in rats and guinea pigs. Oral administration of 6-Acyl-AA-2G to rats resulted in an increase in the plasma AA level. However, the intact form was not detectable in the plasma by high-performance liquid chromatography, indicating its hydrolysis through the process of absorption. After an intravenous injection to rats of 6-Octa-AA-2G as a representative derivative, the intact form rapidly disappeared from the plasma, being followed by a prolonged and marked elevation of the plasma AA level. Various tissue homogenates from guinea pigs were examined for their releasing activity of AA, 2-O-alpha-D-glucopyranosyl-L-Ascorbic acid (AA-2G) and 6-O-acyl-AA from 6-Acyl-AA-2G. High activity was observed in the small intestine. These hydrolytic activities to AA and 6-O-acyl-AA were completely inhibited by castanospermine, an alpha-glucosidase inhibitor, and AA-2G was observed as the only resulting hydrolysate, suggesting the participation of alpha-glucosidase and esterase in the in vivo hydrolysis of 6-Acyl-AA-2G. 6-Octa-AA-2G was found to exhibit an obvious therapeutic effect in scorbutic guinea pigs from its repeated oral administration. These results indicate that 6-Acyl-AA-2G is a readily available source of AA activity in vivo, and may be useful as an effective pharmacological agent and as a promising food additive.