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Ascorbic Acids
The Experts below are selected from a list of 264 Experts worldwide ranked by ideXlab platform
Wageh Sobhy Darwish – 1st expert on this subject based on the ideXlab platform
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identification of lead produced lipid hydroperoxides in human hepg2 cells and protection using rosmarinic and Ascorbic Acids with a reference to their regulatory roles on nrf2 keap1 antioxidant pathway
Chemico-Biological Interactions, 2019Co-Authors: Yonghan Li, Wageh Sobhy Darwish, Zhen Chen, Yue Wu, Suzuki Hirotaka, Hitoshi ChibaAbstract:Abstract Lead (Pb) is one of the toxic heavy metals that have several toxicological implications including cytotoxicities and oxidative stress. The release of reactive oxygen species (ROS) usually initiates lipid peroxidation and resulting in inflammation and tissue injury. However, the detailed identification of the Pb-produced lipid hydroperoxides has received little attention. Furthermore, the mechanisms behind such effects are less informed. Therefore, this study firstly investigated Pb-produced lipid hydroperoxides in human HepG2 cells using LC/MS. The effects of Pb on the antioxidant enzymes were additionally examined using qPCR and their dependent activities. As a protection trial, the ameliorative effects of rosmarinic (RMA) and Ascorbic (ASA) Acids on Pb-induced cytotoxicity and oxidative stress and their regulatory effects on Nrf2/Keap1 pathway were investigated. The achieved results confirmed cytotoxicity and oxidative damage of Pb on HepG2 cells. In addition, 20 lipid hydroperoxides (LOOH) were identified including 11 phosphatidylcholine hydroperoxides (PCOOH), 5 triacylglycerol hydroperoxides (TGOOH) and 4 cholesteryl ester hydroperoxides (CEOOH). The most dominant LOOH species were PCOOH 34:2, PCOOH 34:3, PCOOH 38:7, TGOOH 60:14, TGOOH 60:15, CEOOH 18:3 and CEOOH 20:4. Pb significantly downregulated Nrf2-regulated antioxidant enzymes at both the pretranscriptional and functional levels. Co-exposure of HepG2 cells to RMA and ASA significantly reduced Pb-produced adverse outcomes. This protection occurred via activation Nrf2-Keap1 antioxidant pathway.
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determination of polycyclic aromatic hydrocarbon content in heat treated meat retailed in egypt health risk assessment benzo a pyrene induced mutagenicity and oxidative stress in human colon caco 2 cells and protection using rosmarinic and Ascorbic a
Food Chemistry, 2019Co-Authors: Wageh Sobhy Darwish, Abdelazim Elsayed Elhelaly, Hitoshi Chiba, Waleed R ElghareebAbstract:Abstract This study was undertaken to estimate the concentrations of the formed polycyclic aromatic hydrocarbons (PAHs) in heat-treated (boiled, pan-fried and grilled) meats collected from Egypt. Dietary intakes and cancer risks of PAHs among Egyptian adults were calculated. Benzo[a]pyrene (B[a]P)-induced mutagenicity and oxidative stress in human colon (CaCo-2) cell line and mechanisms behind such effects were also investigated. Finally, protection trials using rosmarinic (RMA) and Ascorbic Acids (ASA) were carried out. The results indicated formation of PAHs at high levels in the heat-treated meats. Calculated incremental life time cancer risk among Egyptian adults were 7.05179E−07, 7.00604 E−06 and 1.86069 E−05 due to ingestion of boiled, pan-fried and grilled meats, respectively. B[a]P-exposed CaCo-2 cells had high abilities for mutagenicity (490.05 ± 21.37 His + revertants) and production of reactive oxygen species. RMA and ASA protected CaCo-2 cells via reduction of B[a]P-induced mutagenicity and oxidative stress and upregulation of phase II detoxification enzymes and xenobiotic transporters.
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estimation of cadmium content in egyptian foodstuffs health risk assessment biological responses of human hepg2 cells to food relevant concentrations of cadmium and protection trials using rosmarinic and Ascorbic Acids
Environmental Science and Pollution Research, 2019Co-Authors: Wageh Sobhy Darwish, Hitoshi Chiba, Abdelazim Elsayed ElhelalyAbstract:Cadmium (Cd) is an environmental pollutant that can get entry into human body via ingestion of contaminated foods causing multiple organ damage. This study aimed at monitoring Cd residues in 20 foodstuffs of animal origin that are commonly consumed in Egypt. Health risk assessment was conducted via calculation of Cd dietary intakes and non-carcinogenic target hazard quotient. An in vitro approach was performed to investigate the constitutive effects of Cd on human hepatoma (HepG2) cells under food-relevant concentrations. Trials to reduce Cd-induced adverse effects on HepG2 cells were done using rosmarinic (RMA) and Ascorbic Acids (ASA). The achieved results indicated contamination of the tested foodstuffs with Cd at high levels with potential human health hazards. Cd at food-relevant concentrations caused significant cytotoxicity to HepG2 cells. This may be attributed to induction of oxidative stress and inflammation, as indicated by the overexpression of stress and inflammatory markers. At the same time, Cd downregulated xenobiotic transporters and upregulated the proliferation factors. Co-exposure of HepG2 cells to Cd and micronutrients such as RMA and ASA led to recovery of cells from the oxidative damage, and subsequently cell viability was strongly improved. RMA and ASA ameliorated the biological responses of HepG2 cells to Cd exposure.
Alfredo Montano – 2nd expert on this subject based on the ideXlab platform
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fermented vegetables containing benzoic and Ascorbic Acids as additives benzene formation during storage and impact of additives on quality parameters
Journal of Agricultural and Food Chemistry, 2011Co-Authors: Francisco Javier Casado, Antonio Higinio Sanchez, Antonio De Castro, Luis Rejano, Victor Manuel Beato, Alfredo MontanoAbstract:Chemical and sensorial changes related to the use of benzoates and Ascorbic acid as additives in packed fermented vegetables were investigated. For this, three selected vegetables (green olives, cucumbers, and caperberries) stored under different conditions (glass or plastic containers, ambient or refrigerated storage) were used. In all cases, benzoic acid remained unchanged (glass bottle) or decreased slightly (plastic pouch) at prolonged storage. Ascorbic acid was partially or totally degraded during storage, the degradation rate depending on the storage conditions and the vegetable matrix. Benzene levels higher than 10 μg/L were found in cucumbers and caperberries containing both additives, but only when packed in plastic pouches and after prolonged storage at room temperature. In these conditions, an appreciable browning of brine, related to AA degradation, was also found. The use of benzoate alone had a significant influence on vegetable color, but flavor was not significantly affected at the benzoat…
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Fermented vegetables containing benzoic and Ascorbic Acids as additives: benzene formation during storage and impact of additives on quality parameters.
Journal of Agricultural and Food Chemistry, 2011Co-Authors: Francisco Javier Casado, Antonio Higinio Sanchez, Antonio De Castro, Luis Rejano, Victor Manuel Beato, Alfredo MontanoAbstract:Chemical and sensorial changes related to the use of benzoates and Ascorbic acid as additives in packed fermented vegetables were investigated. For this, three selected vegetables (green olives, cucumbers, and caperberries) stored under different conditions (glass or plastic containers, ambient or refrigerated storage) were used. In all cases, benzoic acid remained unchanged (glass bottle) or decreased slightly (plastic pouch) at prolonged storage. Ascorbic acid was partially or totally degraded during storage, the degradation rate depending on the storage conditions and the vegetable matrix. Benzene levels higher than 10 μg/L were found in cucumbers and caperberries containing both additives, but only when packed in plastic pouches and after prolonged storage at room temperature. In these conditions, an appreciable browning of brine, related to AA degradation, was also found. The use of benzoate alone had a significant influence on vegetable color, but flavor was not significantly affected at the benzoate levels tested. On the basis of the present study, benzoates should be removed from fermented vegetable formulations containing Ascorbic acid to eliminate possible benzene formation during long-term storage.
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fermented vegetables containing benzoic and Ascorbic Acids as additives benzene formation during storage and impact of additives on quality parameters
Journal of Agricultural and Food Chemistry, 2011Co-Authors: Francisco Javier Casado, Antonio Higinio Sanchez, Antonio De Castro, Luis Rejano, Victor Manuel Beato, Alfredo MontanoAbstract:Chemical and sensorial changes related to the use of benzoates and Ascorbic acid as additives in packed fermented vegetables were investigated. For this, three selected vegetables (green olives, cu…
Abdelazim Elsayed Elhelaly – 3rd expert on this subject based on the ideXlab platform
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determination of polycyclic aromatic hydrocarbon content in heat treated meat retailed in egypt health risk assessment benzo a pyrene induced mutagenicity and oxidative stress in human colon caco 2 cells and protection using rosmarinic and Ascorbic a
Food Chemistry, 2019Co-Authors: Wageh Sobhy Darwish, Abdelazim Elsayed Elhelaly, Hitoshi Chiba, Waleed R ElghareebAbstract:Abstract This study was undertaken to estimate the concentrations of the formed polycyclic aromatic hydrocarbons (PAHs) in heat-treated (boiled, pan-fried and grilled) meats collected from Egypt. Dietary intakes and cancer risks of PAHs among Egyptian adults were calculated. Benzo[a]pyrene (B[a]P)-induced mutagenicity and oxidative stress in human colon (CaCo-2) cell line and mechanisms behind such effects were also investigated. Finally, protection trials using rosmarinic (RMA) and Ascorbic Acids (ASA) were carried out. The results indicated formation of PAHs at high levels in the heat-treated meats. Calculated incremental life time cancer risk among Egyptian adults were 7.05179E−07, 7.00604 E−06 and 1.86069 E−05 due to ingestion of boiled, pan-fried and grilled meats, respectively. B[a]P-exposed CaCo-2 cells had high abilities for mutagenicity (490.05 ± 21.37 His + revertants) and production of reactive oxygen species. RMA and ASA protected CaCo-2 cells via reduction of B[a]P-induced mutagenicity and oxidative stress and upregulation of phase II detoxification enzymes and xenobiotic transporters.
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estimation of cadmium content in egyptian foodstuffs health risk assessment biological responses of human hepg2 cells to food relevant concentrations of cadmium and protection trials using rosmarinic and Ascorbic Acids
Environmental Science and Pollution Research, 2019Co-Authors: Wageh Sobhy Darwish, Hitoshi Chiba, Abdelazim Elsayed ElhelalyAbstract:Cadmium (Cd) is an environmental pollutant that can get entry into human body via ingestion of contaminated foods causing multiple organ damage. This study aimed at monitoring Cd residues in 20 foodstuffs of animal origin that are commonly consumed in Egypt. Health risk assessment was conducted via calculation of Cd dietary intakes and non-carcinogenic target hazard quotient. An in vitro approach was performed to investigate the constitutive effects of Cd on human hepatoma (HepG2) cells under food-relevant concentrations. Trials to reduce Cd-induced adverse effects on HepG2 cells were done using rosmarinic (RMA) and Ascorbic Acids (ASA). The achieved results indicated contamination of the tested foodstuffs with Cd at high levels with potential human health hazards. Cd at food-relevant concentrations caused significant cytotoxicity to HepG2 cells. This may be attributed to induction of oxidative stress and inflammation, as indicated by the overexpression of stress and inflammatory markers. At the same time, Cd downregulated xenobiotic transporters and upregulated the proliferation factors. Co-exposure of HepG2 cells to Cd and micronutrients such as RMA and ASA led to recovery of cells from the oxidative damage, and subsequently cell viability was strongly improved. RMA and ASA ameliorated the biological responses of HepG2 cells to Cd exposure.