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B. Rydqvist - One of the best experts on this subject based on the ideXlab platform.
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Action Potential and Sodium Current in the Slowly and Rapidly Adapting Stretch Receptor Neurons of the Crayfish (Astacus Astacus)
Journal of Neurophysiology, 1998Co-Authors: Nuhan Purali, B. RydqvistAbstract:Purali, Nuhan and Bo Rydqvist. Action potential and sodium current in the slowly and rapidly adapting stretch receptor neurons of the crayfish (Astacus Astacus). J. Neurophysiol. 80: 2121–2132, 1998. Action potentials (APs) and sodium current from the slowly and the rapidly adapting stretch receptor neurons in the crayfish (Astacus Astacus) were recorded with a two microelectrode voltage- and current-clamp technique. In the rapidly adapting neuron the APs had a duration of 3.2 ± 0.2 ms (means ± SE) and an amplitude of 55.2 ± 1.5 mV. In the slowly adapting receptor neuron APs had a duration of 4.1 ± 0.2 ms and an amplitude 79.9 ± 2.0 mV. APs in the rapidly adapting neuron had a larger amplitude if they were recorded from the axon. In the rapidly adapting neuron adaptation of the impulse response was prolonged by hyperpolarization or by exposure to scorpion venom. Also, sinusoidal current stimulation added to the current steps prevented impulse adaptation. Block of the potassium currents in the slowly adapt...
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Action Potential and Sodium Current in the Slowly and Rapidly Adapting Stretch Receptor Neurons of the Crayfish (Astacus Astacus)
Journal of neurophysiology, 1998Co-Authors: Nuhan Purali, B. RydqvistAbstract:Purali, Nuhan and Bo Rydqvist. Action potential and sodium current in the slowly and rapidly adapting stretch receptor neurons of the crayfish (Astacus Astacus). J. Neurophysiol. 80: 2121–2132, 199...
Nuhan Purali - One of the best experts on this subject based on the ideXlab platform.
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Action Potential and Sodium Current in the Slowly and Rapidly Adapting Stretch Receptor Neurons of the Crayfish (Astacus Astacus)
Journal of Neurophysiology, 1998Co-Authors: Nuhan Purali, B. RydqvistAbstract:Purali, Nuhan and Bo Rydqvist. Action potential and sodium current in the slowly and rapidly adapting stretch receptor neurons of the crayfish (Astacus Astacus). J. Neurophysiol. 80: 2121–2132, 1998. Action potentials (APs) and sodium current from the slowly and the rapidly adapting stretch receptor neurons in the crayfish (Astacus Astacus) were recorded with a two microelectrode voltage- and current-clamp technique. In the rapidly adapting neuron the APs had a duration of 3.2 ± 0.2 ms (means ± SE) and an amplitude of 55.2 ± 1.5 mV. In the slowly adapting receptor neuron APs had a duration of 4.1 ± 0.2 ms and an amplitude 79.9 ± 2.0 mV. APs in the rapidly adapting neuron had a larger amplitude if they were recorded from the axon. In the rapidly adapting neuron adaptation of the impulse response was prolonged by hyperpolarization or by exposure to scorpion venom. Also, sinusoidal current stimulation added to the current steps prevented impulse adaptation. Block of the potassium currents in the slowly adapt...
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Action Potential and Sodium Current in the Slowly and Rapidly Adapting Stretch Receptor Neurons of the Crayfish (Astacus Astacus)
Journal of neurophysiology, 1998Co-Authors: Nuhan Purali, B. RydqvistAbstract:Purali, Nuhan and Bo Rydqvist. Action potential and sodium current in the slowly and rapidly adapting stretch receptor neurons of the crayfish (Astacus Astacus). J. Neurophysiol. 80: 2121–2132, 199...
Pavel Kozák - One of the best experts on this subject based on the ideXlab platform.
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Label-free protein quantification in freshly ejaculated versus post-mating spermatophores of the noble crayfish Astacus Astacus.
Journal of proteomics, 2015Co-Authors: Hamid Niksirat, Antonín Kouba, Peter James, Lise-lotte Andersson, Pavel KozákAbstract:Crayfish spermatophores are deposited on the body surface of the female during mating and remain there for a period of time before fertilization ensues. Post-mating changes in protein expression level in the noble crayfish Astacus Astacus spermatophore were quantified. In-gel digestion and high resolution mass spectrometry were used for label-free protein quantification. One hundred twelve proteins were identified in the spermatophore of noble crayfish. After 7days of storage on the body of the female, 6 proteins were identified in the post-mating spermatophore that showed significant up-regulation and 4 significant down-regulations (p
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comparative ultrastructure of the spermatozoa of three crayfish species austropotamobius torrentium pacifAstacus leniusculus and Astacus Astacus decapoda astacidae
Journal of Morphology, 2013Co-Authors: Hamid Niksirat, Antonín Kouba, Marek Rodina, Pavel KozákAbstract:This study reports about the spermatozoal ultrastructure of three species of astacid crayfish, i.e., the stone crayfish Austropotamobius torrentium, signal crayfish PacifAstacus leniusculus, and noble crayfish Astacus Astacus. The acrosome is a cup shaped and electron-dense structure at the anterior of the spermatozoon and comprises three layers of differing electron densities filled with parallel filaments that extend from the base to the apical zone. The acrosome was significantly longer in A. Astacus than in P. leniusculus and the shortest acrosome belongs to A. torrentium. The width of the acrosome was significantly narrower in A. torrentium than in P. leniusculus and the widest acrosome belongs to A. Astacus. The L:W ratio was significantly greater in A. torrentium than in P. leniusculus and the lowest ratio belongs to A. Astacus. Radial arms are visible on each side of the acrosome or nucleus in sagittal view and wrap around the spermatozoon. Each radial arm comprises a parallel bundle of microtubules arranged along the long axis within a sheath. The nucleus, with decondensed material, is located in the posterior of the cell. All parts of the spermatozoon are tightly enclosed within an extracellular capsule. Despite a well-conserved general structure and similarity of pattern among these spermatozoa, differences in the dimensions of the acrosome within the studied species may be useful to help distinguish the different crayfish species. J. Morphol., 2013. © 2013 Wiley Periodicals, Inc.
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Comparison of Diurnal and Nocturnal Activity Between Noble Crayfish ( Astacus Astacus) and Spinycheek Crayfish ( Orconectes limosus)
2010Co-Authors: Martin Musil, Pavel KozákAbstract:Several studies have shown that American crayfish species are more active during the day compared to European species. Spinycheek crayfish ( Orconectes limosus) are known as an aggressive nocturnal species with higher daytime activity than noble crayfish (Astacus Astacus). The aim of the present study was to compare diurnal and nocturnal activity of these two species under laboratory conditions with the use of video recording techniques. Three groups were included: noble crayfish only, spinycheek crayfish only, and a mixture of both species. Substantial differences were found between species. Higher (P < 0.05) diurnal and nocturnal activities were recorded for spinycheek crayfish than for noble crayfish, with spinycheek females being more active than males, and higher ( P < 0.05) nocturnal than diurnal activity in noble crayfish when they were kept in same-species enclosures. However, the above activities were modified when both species were kept together, with an increase in diurnal activity noted in noble crayfish, while nocturnal activity was similar between species. A higher activity during the day might influence the vulnerability of noble crayfish to diurnal predators. (Keywords.— Astacus Astacus; interaction; Orconectes limosus; shelter use; video-tracking).
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the effect of inter and intra specific competition on survival and growth rate of native juvenile noble crayfish Astacus Astacus and alien spiny cheek crayfish orconectes limosus
Hydrobiologia, 2007Co-Authors: Pavel Kozák, Milos Buřic, Tomas Policar, J Hamackova, A LepicovaAbstract:Juvenile noble crayfish, Astacus Astacus and spiny-cheek crayfish, Orconectes limosus were reared from the 2nd and the 3rd developmental stage (i.e. since the stage of independence), respectively, until the end of the growing season. The first period revealed very low survival rate of O. limosus in the communal stock (7 ± 6.2%), probably caused by predation of A. Astacus. Separately reared O. limosus proved significantly higher survival rate (49 ± 9.2%) than those in the communal stock with A. Astacus. The latter species also showed the highest survival rate in this case (90 ± 7.1%). This was not found to differ from the survival rate of separately reared noble crayfish (86 ± 3.1%). In general, both the significant effect of bigger size of A. Astacus than that of O. limosus and higher intra-specific cannibalism of O. limosus were reflected during the first phase of rearing. Size and weight of both species were equivalent 11 weeks after hatching (8 weeks of rearing). The higher cumulative survival rate was reached in the intraspecific culture of A. Astacus (90%) while low cumulative survival of both species was found in communal stock (less than 50%) in the second phase of rearing. At the end of the growing season, 19-week-old O. limosus attained a higher mean size and weight but were more variable in the group (29.3 ± 5.17 mm and 616.4 ± 412.53 mg) than A. Astacus (21.2 ± 1.82 mm and 229.9 ± 59.45 mg). Single and communal stock did not differ. Growth of O. limosus was much faster than that of A. Astacus.
A Lepicova - One of the best experts on this subject based on the ideXlab platform.
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the effect of inter and intra specific competition on survival and growth rate of native juvenile noble crayfish Astacus Astacus and alien spiny cheek crayfish orconectes limosus
Hydrobiologia, 2007Co-Authors: Pavel Kozák, Milos Buřic, Tomas Policar, J Hamackova, A LepicovaAbstract:Juvenile noble crayfish, Astacus Astacus and spiny-cheek crayfish, Orconectes limosus were reared from the 2nd and the 3rd developmental stage (i.e. since the stage of independence), respectively, until the end of the growing season. The first period revealed very low survival rate of O. limosus in the communal stock (7 ± 6.2%), probably caused by predation of A. Astacus. Separately reared O. limosus proved significantly higher survival rate (49 ± 9.2%) than those in the communal stock with A. Astacus. The latter species also showed the highest survival rate in this case (90 ± 7.1%). This was not found to differ from the survival rate of separately reared noble crayfish (86 ± 3.1%). In general, both the significant effect of bigger size of A. Astacus than that of O. limosus and higher intra-specific cannibalism of O. limosus were reflected during the first phase of rearing. Size and weight of both species were equivalent 11 weeks after hatching (8 weeks of rearing). The higher cumulative survival rate was reached in the intraspecific culture of A. Astacus (90%) while low cumulative survival of both species was found in communal stock (less than 50%) in the second phase of rearing. At the end of the growing season, 19-week-old O. limosus attained a higher mean size and weight but were more variable in the group (29.3 ± 5.17 mm and 616.4 ± 412.53 mg) than A. Astacus (21.2 ± 1.82 mm and 229.9 ± 59.45 mg). Single and communal stock did not differ. Growth of O. limosus was much faster than that of A. Astacus.
Kenneth Söderhäll - One of the best experts on this subject based on the ideXlab platform.
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The origin of two crayfish plague (Aphanomyces astaci) epizootics in Finland on noble crayfish, Astacus Astacus
Annales Zoologici Fennici, 1998Co-Authors: Pia Vennerström, Kenneth Söderhäll, Lage CereniusAbstract:A fungus identified as Aphanomyces astaci was isolated from two crayfish plague epizootics among noble crayfish, Astacus Astacus, in Central Finland. The isolated fungal strains from Vaikkojoki, Ka ...
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Saprolegnia parasitica and its virulence on three different species of freshwater crayfish
Aquaculture, 1994Co-Authors: Javier Diéguez-uribeondo, Lage Cerenius, Kenneth SöderhällAbstract:Abstract Saprolegnia parasitica isolated from freshwater crayfish Astacus leptodactylus was investigated for its ability to infect and kill three different species of crayfish, Astacus Astacus, PacifAstacus leniusculus and Procambarus clarkii . Healthy (non-injured) crayfish challenged with zoospores of S. parasitica became infected and ca. 20% died as a result of such infection. If the epicuticle of the crayfish was abrased gently before challenge with zoospores, mortality increased significantly, ca. 3 times. There were no significant differences in susceptibility between the three species of crayfish tested against S. parasitica .
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β-1,3-glucan-binding Proteins From Plasma of the Fresh-water Crayfishes Astacus Astacus and Procambarus Clarkii
Journal of Crustacean Biology, 1993Co-Authors: Bernard Duvic, Kenneth SöderhällAbstract:ABSTRACT Two fresh-water crayfishes, Astacus Astacus and Procambarus clarkii, and a marine crab, Carcinus maenas, were examined for the presence of β-1,3-glucan-binding proteins in their plasma. By the use of a monospecific rabbit polyclonal antiserum to the β-1,3-glucan-binding protein of PacifAstacus leniusculus, 2 proteins from the plasma of Astacus Astacus with molecular masses of about 105 kDa and 95 kDa as well as a 100-kDa protein in the plasma of Procambarus clarkii could be detected. No protein in plasma of Carcinus maenas cross-reacted with the anti-PacifAstacus leniusculus β-1,3-glucan-binding protein antibodies. The cross-reactive proteins from plasma of Astacus Astacus were purified by immuno-affinity chromatography. The isolated proteins were homogenous as judged by electrophoresis on SDS-PAGE. Isoelectric focusing of the purified proteins gave pIs of about 6.1 and 6.4. The purified proteins from the plasma of Astacus Astacus enhanced the activity of a hemocyte lysate supernatant-derived phenoloxidase of PacifAstacus leniusculus as did the β-1,3-glucanbinding protein of PacifAstacus leniusculus.
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Beta-1,3-glucan binding proteins from plasma of the fresh-water crayfishes Astacus Astacus and Procambarus clarkii
Journal of Crustacean Biology, 1993Co-Authors: Bernard Duvic, Kenneth SöderhällAbstract:Two fresh-water crayfishes, Astacus Astacus and Procambarus clarkii, and a marine crab, Carcinus maenas, were examined for the presence of beta-1,3-glucan-binding proteins in their plasma. By the use of a monospecific rabbit polyclonal antiserum to the beta-1,3-glucan-binding protein of PacifAstacus leniusculus, 2 proteins from the plasma of Astacus Astacus with molecular masses of about 105 kDa and 95 kDa as well as a 100-kDa protein in the plasma of Procambarus clarkii could be detected. No protein in plasma of Carcinus maenas cross-reacted with the anti-PacifAstacus leniusculus beta-1,3-glucan-binding protein antibodies. The cross-reactive proteins from plasma of Astacus Astacus were purified by immuno-affinity chromatography. The isolated proteins were homogenous as judged by electrophoresis on SDS-PAGE. Isoelectric focusing of the purified proteins gave pIs of about 6.1 and 6.4. The purified proteins from the plasma of Astacus Astacus enhanced the activity of a hemocyte lysate supernatant-derived phenoloxidase of PacifAstacus leniusculus as did the beta-1,3-glucan-binding protein of PacifAstacus leniusculus.