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Mario Vaneechoutte - One of the best experts on this subject based on the ideXlab platform.
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gardnerella vaginalis enhances Atopobium vaginae viability in an in vitro model
Frontiers in Cellular and Infection Microbiology, 2020Co-Authors: Joana Castro, Mario Vaneechoutte, Piet Cools, A Rosca, Nuno CercaAbstract:Bacterial vaginosis (BV) is the most common vaginal infection among women of reproductive age. A hallmark of BV is the presence of a highly structured polymicrobial biofilm on the vaginal epithelium, presumably initiated by facultative anaerobes of the genus Gardnerella, which then becomes a scaffold for other species to adhere to. One of the species often found incorporated in Gardnerella mediated biofilms is Atopobium vaginae. Interestingly, A. vaginae is very rarely found without the presence of Gardnerella. However, not much is known regarding the interactions between A. vaginae and Gardnerella species. This study assessed biological interactions between Gardnerella vaginalis and A. vaginae. In our in vitro model, by using specific Gardnerella and A. vaginae Peptide Nucleic Acid (PNA)-Fluorescence In Situ Hybridization (FISH) probes, we confirmed that A. vaginae was able to incorporate a pre-formed G. vaginalis biofilm, accounting for up to 20% of the total number of biofilm cells. However, our findings showed that almost 92% of A. vaginae cells lost viability after 48 h of mono-species planktonic growth, but were able to maintain viability when co-cultured with Gardnerella or after pre-conditioning with cell-free supernatant of Gardnerella cultures. While the in vitro conditions are very different from the in vivo microenvironment, this study contributes to a better understanding of why A. vaginae vaginal colonization rarely occurs in the absence of Gardnerella. Overall, this highlights the importance of microbial interactions between BV-associated bacteria and demands more studies focused on the polymicrobial bacterial communities found in BV.
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Gardnerella vaginalis enhances Atopobium vaginae viability in vitro
2019Co-Authors: Castro Joana, Mario Vaneechoutte, Piet Cools, Rosca A., Cerca NunoAbstract:Background: Bacterial vaginosis (BV) is a clinical condition characterized by a dramatic shift in the vaginal microflora from the beneficial lactobacilli to a polymicrobial flora, consisting of strictly and facultatively anaerobic bacteria. It is noteworthy that a hallmark of BV is the presence of a highly structured polymicrobial biofilm on the vaginal epithelium, presumably initiated by a facultative anaerobe, Gardnerella vaginalis, which then become a scaffold for other species to adhere. While not much is known about multi- species interactions within BV biofilms, Atopobium vaginae is often associated with G. vaginalis biofilms and is rarely detected without G. vaginalis.This study was supported by the Portuguese Foundation for Science and Technology (FCT) by the funded project PTDC/BIA-MIC/28271/2017, under the scope of COMPETE2020 (POCI-01-0145- FEDER-028271) and by the strategic funding of unit UID/BIO/04469/2019.info:eu-repo/semantics/publishedVersio
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P07.16 The importance of Atopobium vaginae in bacterial vaginosis-associated biofilm
Sexually Transmitted Infections, 2015Co-Authors: Liselotte Hardy, Vicky Jespers, Lambert Mwambarangwe, Viateur Musengamana, Mario Vaneechoutte, Saïd Abdellati, I De Baetselier, J Van De Wijgert, Tania CrucittiAbstract:Introduction In case of bacterial vaginosis (BV), lactobacilli are outnumbered by anaerobic bacteria. These bacteria have been described to form a polymicrobial biofilm (“clue cells”) attached to the vaginal epithelium, possibly explaining the decreased sensitivity to antibiotic treatment and the frequent chronicity of BV. A better understanding of this biofilm may contribute to more precise delineation, and consequently to better diagnosis and treatment of BV. We used Fluorescence In Situ Hybridization (FISH) to study the presence of Atopobium vaginae (Av) and Gardnerella vaginalis (Gv) biofilm, in relation to the status of the vaginal microbiome (according to Nugent score). Methods Duplicates of 461 vaginal slides of 120 participants, participating in a contraceptive vaginal ring trial in Rwanda, were evaluated according to Nugent score after Gram staining and by FISH with species-specific probes for Av and Gv. Results In the majority of samples (59.6%) with Nugent score 7–10 (BV diagnosis), a Gv+Av biofilm was visualised by FISH. In these BV samples, 7% had no Gv and Av present, in 9% Gv and/or Av was present but only planktonic, 18.6% had Gv biofilm but no Av present and 5.8% Gv biofilm with Av planktonic only. When Av and/or Gv were planktonic in the vagina and not part of a biofilm, the Nugent score was low, defining a healthy vaginal microbiome. However the probability of having a disturbed vaginal microbiome (Nugent 7–10) was increased (p A. vaginae was part of the biofilm (p Conclusion Our study focusing on Gv and Av, shows that these are two major players in a polymicrobial condition. It confirms the importance of Gv - biofilm in BV and strongly indicates that Av plays an important role in BV-associated biofilm. Disclosure of interest statement This work was supported by European and Developing Countries Clinical Trials Partnerships (EDCTP), by Combined Highly Active Anti-Retroviral Microbicides (CHAARM) and by Dormeur Investment Service. No pharmaceutical grants were received in the development of this study.
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unravelling the bacterial vaginosis associated biofilm a multiplex gardnerella vaginalis and Atopobium vaginae fluorescence in situ hybridization assay using peptide nucleic acid probes
PLOS ONE, 2015Co-Authors: Liselotte Hardy, Vicky Jespers, Nassira Dahchour, Lambert Mwambarangwe, Viateur Musengamana, Mario Vaneechoutte, Tania CrucittiAbstract:Bacterial vaginosis (BV), a condition defined by increased vaginal discharge without significant inflammation, is characterized by a change in the bacterial composition of the vagina. Lactobacillus spp., associated with a healthy vaginal microbiome, are outnumbered by BV-associated organisms. These bacteria could form a polymicrobial biofilm which allows them to persist in spite of antibiotic treatment. In this study, we examined the presence of Gardnerella vaginalis and Atopobium vaginae in vaginal biofilms using Peptide Nucleic Acid (PNA) probes targeting these bacteria. For this purpose, we developed three new PNA probes for A. vaginae. The most specific A. vaginae probe, AtoITM1, was selected and then used in an assay with two existing probes, Gard162 and BacUni-1, to evaluate multiplex FISH on clinical samples. Using quantitative polymerase chain reaction (qPCR) as the gold standard, we demonstrated a sensitivity of 66.7% (95% confidence interval: 54.5% - 77.1%) and a specificity of 89.4% (95% confidence interval: 76.1% - 96%) of the new AtoITM1 probe. FISH enabled us to show the presence of a polymicrobial biofilm in bacterial vaginosis, in which Atopobium vaginae is part of a Gardnerella vaginalis-dominated biofilm. We showed that the presence of this biofilm is associated with high bacterial loads of A. vaginae and G. vaginalis.
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Atopobium deltae sp. nov., isolated from the blood of a patient with Fournier’s gangrene
International Journal of Systematic and Evolutionary Microbiology, 2014Co-Authors: Piet Cools, Mario Vaneechoutte, Matthijs Oyaert, Emmanuel De Laere, Steven VervaekeAbstract:A Gram-stain-positive, obligately anaerobic, short rod, designated strain HHRM1715T, was isolated from the blood of a patient with Fournier’s gangrene, complicated by sepsis. On the basis of 16S rRNA gene sequence analysis, strain HHRM1715T was shown to belong to the genus Atopobium and was most closely related to Atopobium minutum (95 % similarity). The results of 16S rRNA-gene-based phylogenetic analysis, cellular fatty acid analysis and differential biochemical tests, showed that strain HHRM1715T represented a novel species of the genus Atopobium . We therefore describe Atopobium deltae sp. nov. with HHRM1715T ( = LMG 27987T = CCUG 65171T) as the type strain and propose an emended description of the genus Atopobium with regard to the DNA G+C content.
Piet Cools - One of the best experts on this subject based on the ideXlab platform.
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gardnerella vaginalis enhances Atopobium vaginae viability in an in vitro model
Frontiers in Cellular and Infection Microbiology, 2020Co-Authors: Joana Castro, Mario Vaneechoutte, Piet Cools, A Rosca, Nuno CercaAbstract:Bacterial vaginosis (BV) is the most common vaginal infection among women of reproductive age. A hallmark of BV is the presence of a highly structured polymicrobial biofilm on the vaginal epithelium, presumably initiated by facultative anaerobes of the genus Gardnerella, which then becomes a scaffold for other species to adhere to. One of the species often found incorporated in Gardnerella mediated biofilms is Atopobium vaginae. Interestingly, A. vaginae is very rarely found without the presence of Gardnerella. However, not much is known regarding the interactions between A. vaginae and Gardnerella species. This study assessed biological interactions between Gardnerella vaginalis and A. vaginae. In our in vitro model, by using specific Gardnerella and A. vaginae Peptide Nucleic Acid (PNA)-Fluorescence In Situ Hybridization (FISH) probes, we confirmed that A. vaginae was able to incorporate a pre-formed G. vaginalis biofilm, accounting for up to 20% of the total number of biofilm cells. However, our findings showed that almost 92% of A. vaginae cells lost viability after 48 h of mono-species planktonic growth, but were able to maintain viability when co-cultured with Gardnerella or after pre-conditioning with cell-free supernatant of Gardnerella cultures. While the in vitro conditions are very different from the in vivo microenvironment, this study contributes to a better understanding of why A. vaginae vaginal colonization rarely occurs in the absence of Gardnerella. Overall, this highlights the importance of microbial interactions between BV-associated bacteria and demands more studies focused on the polymicrobial bacterial communities found in BV.
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Gardnerella vaginalis enhances Atopobium vaginae viability in vitro
2019Co-Authors: Castro Joana, Mario Vaneechoutte, Piet Cools, Rosca A., Cerca NunoAbstract:Background: Bacterial vaginosis (BV) is a clinical condition characterized by a dramatic shift in the vaginal microflora from the beneficial lactobacilli to a polymicrobial flora, consisting of strictly and facultatively anaerobic bacteria. It is noteworthy that a hallmark of BV is the presence of a highly structured polymicrobial biofilm on the vaginal epithelium, presumably initiated by a facultative anaerobe, Gardnerella vaginalis, which then become a scaffold for other species to adhere. While not much is known about multi- species interactions within BV biofilms, Atopobium vaginae is often associated with G. vaginalis biofilms and is rarely detected without G. vaginalis.This study was supported by the Portuguese Foundation for Science and Technology (FCT) by the funded project PTDC/BIA-MIC/28271/2017, under the scope of COMPETE2020 (POCI-01-0145- FEDER-028271) and by the strategic funding of unit UID/BIO/04469/2019.info:eu-repo/semantics/publishedVersio
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Atopobium deltae sp. nov., isolated from the blood of a patient with Fournier’s gangrene
International Journal of Systematic and Evolutionary Microbiology, 2014Co-Authors: Piet Cools, Mario Vaneechoutte, Matthijs Oyaert, Emmanuel De Laere, Steven VervaekeAbstract:A Gram-stain-positive, obligately anaerobic, short rod, designated strain HHRM1715T, was isolated from the blood of a patient with Fournier’s gangrene, complicated by sepsis. On the basis of 16S rRNA gene sequence analysis, strain HHRM1715T was shown to belong to the genus Atopobium and was most closely related to Atopobium minutum (95 % similarity). The results of 16S rRNA-gene-based phylogenetic analysis, cellular fatty acid analysis and differential biochemical tests, showed that strain HHRM1715T represented a novel species of the genus Atopobium . We therefore describe Atopobium deltae sp. nov. with HHRM1715T ( = LMG 27987T = CCUG 65171T) as the type strain and propose an emended description of the genus Atopobium with regard to the DNA G+C content.
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Atopobium deltae sp. nov., isolated from the blood of a patient with Fournier's gangrene
International Journal of Systematic and Evolutionary Microbiology, 2014Co-Authors: Piet Cools, Mario Vaneechoutte, Matthijs Oyaert, Emmanuel De Laere, Steven VervaekeAbstract:A Gram-stain-positive, obligately anaerobic, short rod, designated strain HHRM1715T, was isolated from the blood of a patient with Fournier’s gangrene, complicated by sepsis. On the basis of 16S rRNA gene sequence analysis, strain HHRM1715T was shown to belong to the genus Atopobium and was most closely related to Atopobium minutum (95 % similarity). The results of 16S rRNA-gene-based phylogenetic analysis, cellular fatty acid analysis and differential biochemical tests, showed that strain HHRM1715T represented a novel species of the genus Atopobium . We therefore describe Atopobium deltae sp. nov. with HHRM1715T ( = LMG 27987T = CCUG 65171T) as the type strain and propose an emended description of the genus Atopobium with regard to the DNA G+C content.
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Sepsis with an Atopobium-Like Species in a Patient with Fournier's Gangrene
Journal of clinical microbiology, 2013Co-Authors: Matthijs Oyaert, Mario Vaneechoutte, Piet Cools, Steven Vervaeke, Joke Breyne, Gert Heyvaert, Anne Vandewiele, Emmanuel De LaereAbstract:Atopobium species are Gram-positive, anaerobic, catalase-negative, fastidious bacteria belonging to the family Coriobacteriaceae. We report the isolation of an Atopobium-like species in a patient with Fournier's gangrene and highlight the role of 16S rRNA gene sequencing in the identification of fastidious organisms in the clinical laboratory.
Werner Mendling - One of the best experts on this subject based on the ideXlab platform.
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An update on the role of Atopobium vaginae in bacterial vaginosis: what to consider when choosing a treatment?
Voprosy ginekologii akušerstva i perinatologii, 2020Co-Authors: Werner Mendling, Valdas Prasauskas, Stephan Biber, Ana Palmeira‑de‑oliveira, ScientificAbstract:Introduction. Bacterial vaginosis (BV) is the most common vaginal disorder in reproductive-age women. The condition is characterised by the replacement of a healthy, lactobacilli-dominated vaginal microbiota by anaerobic and facultative anaerobic bacteria. BV increases the risk of acquisition of STIs and is associated with pregnancy complications. Although the composition of the bacteria in BV varies between individuals, there are some species such as Gardnerella, Atopobium, Mycoplasma, Snethia, Megasphera, Dialister, etc., that are found most frequently. Material and methods. Literature research to the importance of Atopobium vaginae in BV and treatment options. Results. Atopobium (A.) vaginae is an important component of the complex abnormal vaginal flora in BV; even though A. vaginae, like Gardnerella vaginalis, has also been detected in the normal flora, it is much more common in BV patients. A. vaginae has been shown to play an important role in the pathophysiology of BV and is thought to be at least a partial cause of the known negative sequelae. The presence of A. vaginae in the BV-associated biofilms and its resistance to some antimicrobial substances has been described – this seems to have a major impact on treatment outcome. Conclusion. Current scientific data demonstrate that dequalinium chloride (Fluomycin®) is one of the valid therapeutic options for BV treatment, since it displays a broad antimicrobial spectrum against relevant vaginal pathogens, especially against G. vaginalis and A. vaginae, without having safety concerns. Key words: Bacterial vaginosis, Bacterial biofilm, Atopobium vaginae, Metronidazole, Clindamycin, Dequalinium chloride, Microbial resistance
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An update on the role of Atopobium vaginae in bacterial vaginosis: what to consider when choosing a treatment? A mini review
Archives of Gynecology and Obstetrics, 2019Co-Authors: Werner Mendling, Ana Palmeira-de-oliveira, Stephan Biber, Valdas PrasauskasAbstract:Introduction Bacterial vaginosis (BV) is the most common vaginal disorder in reproductive-age women. The condition is characterised by the replacement of a healthy, lactobacilli-dominated vaginal microbiota by anaerobic and facultative anaerobic bacteria. BV increases the risk of acquisition of STIs and is associated with pregnancy complications. Although the composition of the bacteria in BV varies between individuals, there are some species such as Gardnerella , Atopobium , Mycoplasma , Snethia , Megasphera , Dialister , etc., that are found most frequently. Material and Methods Literature research to the importance of Atopobium vaginae in BV and treatment options. Results Atopobium (A.) vaginae is an important component of the complex abnormal vaginal flora in BV; even though A. vaginae , like Gardnerella vaginalis , has also been detected in the normal flora, it is much more common in BV patients. A. vaginae has been shown to play an important role in the pathophysiology of BV and is thought to be at least a partial cause of the known negative sequelae. The presence of A. vaginae in the BV-associated biofilms and its resistance to some antimicrobial substances has been described - this seems to have a major impact on treatment outcome. Conclusion Current scientific data demonstrate that dequalinium chloride (Fluomycin^®) is one of the valid therapeutic options for BV treatment, since it displays a broad antimicrobial spectrum against relevant vaginal pathogens, especially against G. vaginalis and A. vaginae , without having safety concerns.
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An update on the role of Atopobium vaginae in bacterial vaginosis: what to consider when choosing a treatment? A mini review.
Archives of gynecology and obstetrics, 2019Co-Authors: Werner Mendling, Ana Palmeira-de-oliveira, Stephan Biber, Valdas PrasauskasAbstract:Bacterial vaginosis (BV) is the most common vaginal disorder in reproductive-age women. The condition is characterised by the replacement of a healthy, lactobacilli-dominated vaginal microbiota by anaerobic and facultative anaerobic bacteria. BV increases the risk of acquisition of STIs and is associated with pregnancy complications. Although the composition of the bacteria in BV varies between individuals, there are some species such as Gardnerella, Atopobium, Mycoplasma, Snethia, Megasphera, Dialister, etc., that are found most frequently. Literature research to the importance of Atopobium vaginae in BV and treatment options. Atopobium (A.) vaginae is an important component of the complex abnormal vaginal flora in BV; even though A. vaginae, like Gardnerella vaginalis, has also been detected in the normal flora, it is much more common in BV patients. A. vaginae has been shown to play an important role in the pathophysiology of BV and is thought to be at least a partial cause of the known negative sequelae. The presence of A. vaginae in the BV-associated biofilms and its resistance to some antimicrobial substances has been described - this seems to have a major impact on treatment outcome. Current scientific data demonstrate that dequalinium chloride (Fluomycin®) is one of the valid therapeutic options for BV treatment, since it displays a broad antimicrobial spectrum against relevant vaginal pathogens, especially against G. vaginalis and A. vaginae, without having safety concerns.
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In vitro activity of nifuratel on vaginal bacteria: could it be a good candidate for the treatment of bacterial vaginosis?
Antimicrobial agents and chemotherapy, 2011Co-Authors: Giuseppe Togni, Valeria Battini, Bulgheroni Anna, Federico Mailland, Maurizio Caserini, Werner MendlingAbstract:Bacterial vaginosis is characterized by a shift of the physiological flora to a diverse spectrum of bacteria, where Gardnerella vaginalis and Atopobium vaginae are the most important markers. In this study, the antimicrobial activity of nifuratel against G. vaginalis, A. vaginae, and lactobacilli was compared with that of the two currently used antibiotics metronidazole and clindamycin. Results suggest that nifuratel has a better spectrum of activity, being highly active against G. vaginalis and A. vaginae without affecting lactobacilli.
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response of gardnerella vaginalis biofilm to 5 days of moxifloxacin treatment
Fems Immunology and Medical Microbiology, 2011Co-Authors: Alexander Swidsinski, Yvonne Dorffel, Vera Loeningbaucke, Johannes Schilling, Werner MendlingAbstract:Polymicrobial communities are often recalcitrant to antibiotics. We tested whether the polymicrobial Gardnerella vaginalis biofilm can be eradicated with moxifloxacin. Twenty women with bacterial vaginosis were treated with 400 mg moxifloxacin for 5 days. The changes in the occurrence and proportions of Gardnerella, Atopobium and Lactobacillus spp. were assessed using FISH. The bacterial biofilm was investigated using desquamated epithelial cells of spontaneously voided urine and sections of vaginal biopsies. Fifteen of 20 women showed a significant and sustained clinical response to moxifloxacin according to Amsel and Nugent criteria. The concentrations of adherent bacteria decreased significantly. The incidence and proportion of Atopobium declined sustainably. The proportions of Lactobacillus in the biofilm mass increased following therapy. Initially, Gardnerella was the main component of the polymicrobial biofilm. Following treatment, Gardnerella was not accessible to FISH in the urine and vaginal samples of 75% of all women. Ten to 12 weeks after the end of therapy, Gardnerella biofilm was cumulatively present in 40%. This was not due to newly acquired disease, but due to reactivation of the persisting, but biochemically inactive biofilm. Despite clear clinical efficacy, and initially definite suppression of the biofilm, moxifloxacin was, similar to metronidazole, not able to eradicate the Gardnerella vaginalis biofilm in all patients.
Larry J. Forney - One of the best experts on this subject based on the ideXlab platform.
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Fine-scale analysis of 16S rRNA sequences reveals a high level of taxonomic diversity among vaginal Atopobium spp.
Pathogens and disease, 2015Co-Authors: Helena Mendes-soares, Vandhana Krishnan, Matthew L. Settles, Jacques Ravel, Celeste J. Brown, Larry J. ForneyAbstract:Although vaginal microbial communities of some healthy women have high proportions of Atopobium vaginae, the genus Atopobium is more commonly associated with bacterial vaginosis, a syndrome associated with an increased risk of adverse pregnancy outcomes and the transmission of sexually transmitted diseases. Genetic differences within Atopobium species may explain why single species can be associated with both health and disease. We used 16S rRNA gene sequences from previously published studies to explore the taxonomic diversity of the genus Atopobium in vaginal microbial communities of healthy women. Although A. vaginae was the species most commonly found, we also observed three other Atopobium species in the vaginal microbiota, one of which, A. parvulum, was not previously known to reside in the human vagina. Furthermore, we found several potential novel species of the genus Atopobium and multiple phylogenetic clades of A. vaginae. The diversity of Atopobium found in our study, which focused only on samples from healthy women, is greater than previously recognized, suggesting that analysis of samples from women with BV would yield even more diversity. Classification of microbes only to the genus level may thus obfuscate differences that might be important to better understand health or disease.
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characterization of vaginal microbial communities in adult healthy women using cultivation independent methods
Microbiology, 2004Co-Authors: Xia Zhou, Stephen J Bent, Maria Schneider, Catherine C Davis, Mohammed R Islam, Larry J. ForneyAbstract:The normal microbial flora of the vagina plays an important role in preventing genital and urinary tract infections in women. Thus an accurate understanding of the composition and ecology of the ecosystem is important to understanding the aetiology of these diseases. Common wisdom is that lactobacilli dominate the normal vaginal microflora of post-pubertal women. However, this conclusion is based on methods that require cultivation of microbial populations; an approach that is known to yield a biased and incomplete assessment of microbial community structure. In this study cultivation-independent methods were used to analyse samples collected from the mid-vagina of five normal healthy Caucasian women between the ages of 28 and 44. Total microbial community DNA was isolated following resuspension of microbial cells from vaginal swabs. To identify the constituent numerically dominant populations in each community 16S rRNA gene libraries were prepared following PCR amplification using the 8f and 926r primers. From each library, the DNA sequences of approximately 200 16S rRNA clones were determined and subjected to phylogenetic analyses. The diversity and kinds of organisms that comprise the vaginal microbial community varied among women. Species of Lactobacillus appeared to dominate the communities in four of the five women. However, the community of one woman was dominated by Atopobium sp., whereas a second woman had appreciable numbers of Megasphaera sp., Atopobium sp. and Leptotrichia sp., none of which have previously been shown to be common members of the vaginal ecosystem. Of the women whose communities were dominated by lactobacilli, there were two distinct clusters, each of which consisted of a single species. One class consisted of two women with genetically divergent clones that were related to Lactobacillus crispatus, whereas the second group of two women had clones of Lactobacillus iners that were highly related to a single phylotype. These surprising results suggest that culture-independent methods can provide new insights into the diversity of bacterial species found in the human vagina, and this information could prove to be pivotal in understanding risk factors for various infectious diseases.
Steven Vervaeke - One of the best experts on this subject based on the ideXlab platform.
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Atopobium deltae sp. nov., isolated from the blood of a patient with Fournier’s gangrene
International Journal of Systematic and Evolutionary Microbiology, 2014Co-Authors: Piet Cools, Mario Vaneechoutte, Matthijs Oyaert, Emmanuel De Laere, Steven VervaekeAbstract:A Gram-stain-positive, obligately anaerobic, short rod, designated strain HHRM1715T, was isolated from the blood of a patient with Fournier’s gangrene, complicated by sepsis. On the basis of 16S rRNA gene sequence analysis, strain HHRM1715T was shown to belong to the genus Atopobium and was most closely related to Atopobium minutum (95 % similarity). The results of 16S rRNA-gene-based phylogenetic analysis, cellular fatty acid analysis and differential biochemical tests, showed that strain HHRM1715T represented a novel species of the genus Atopobium . We therefore describe Atopobium deltae sp. nov. with HHRM1715T ( = LMG 27987T = CCUG 65171T) as the type strain and propose an emended description of the genus Atopobium with regard to the DNA G+C content.
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Atopobium deltae sp. nov., isolated from the blood of a patient with Fournier's gangrene
International Journal of Systematic and Evolutionary Microbiology, 2014Co-Authors: Piet Cools, Mario Vaneechoutte, Matthijs Oyaert, Emmanuel De Laere, Steven VervaekeAbstract:A Gram-stain-positive, obligately anaerobic, short rod, designated strain HHRM1715T, was isolated from the blood of a patient with Fournier’s gangrene, complicated by sepsis. On the basis of 16S rRNA gene sequence analysis, strain HHRM1715T was shown to belong to the genus Atopobium and was most closely related to Atopobium minutum (95 % similarity). The results of 16S rRNA-gene-based phylogenetic analysis, cellular fatty acid analysis and differential biochemical tests, showed that strain HHRM1715T represented a novel species of the genus Atopobium . We therefore describe Atopobium deltae sp. nov. with HHRM1715T ( = LMG 27987T = CCUG 65171T) as the type strain and propose an emended description of the genus Atopobium with regard to the DNA G+C content.
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Sepsis with an Atopobium-Like Species in a Patient with Fournier's Gangrene
Journal of clinical microbiology, 2013Co-Authors: Matthijs Oyaert, Mario Vaneechoutte, Piet Cools, Steven Vervaeke, Joke Breyne, Gert Heyvaert, Anne Vandewiele, Emmanuel De LaereAbstract:Atopobium species are Gram-positive, anaerobic, catalase-negative, fastidious bacteria belonging to the family Coriobacteriaceae. We report the isolation of an Atopobium-like species in a patient with Fournier's gangrene and highlight the role of 16S rRNA gene sequencing in the identification of fastidious organisms in the clinical laboratory.