The Experts below are selected from a list of 2982 Experts worldwide ranked by ideXlab platform
Deepti Jain - One of the best experts on this subject based on the ideXlab platform.
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Development and validation of RP-HPLC method for simultaneous estimation of Atorvastatin Calcium and fenofibrate in tablet dosage forms
Indian journal of pharmaceutical sciences, 2008Co-Authors: Nilesh Jain, R Raghuwanshi, Deepti JainAbstract:A reverse phase high performance liquid chromatographic method was developed for the simultaneous estimation of Atorvastatin Calcium and fenofibrate in tablet formulation. The separation was achieved by Luna C18 column and methanol:acetate buffer pH 3.7 (82:18 v/v) as mobile phase, at a flow rate of 1.5 ml/min. Detection was carried out at 248 nm. Retention time of Atorvastatin Calcium and fenofibrate was found to be 3.02+0.1 and 9.05+0.2 min, respectively. The method has been validated for linearity, accuracy and precision. Linearity for Atorvastatin Calcium and Fenofibrate were in the range of 1-5 μg/ml and 16-80 μg/ml, respectively. The mean recoveries obtained for Atorvastatin Calcium and fenofibrate were 101.76% and 100.06%, respectively. Developed method was found to be accurate, precise, selective and rapid for simultaneous estimation of Atorvastatin Calcium and fenofibrate in tablets.
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simultaneous spectrophotometric determination of Atorvastatin Calcium and amlodipine besylate in tablets
Indian Journal of Pharmaceutical Sciences, 2006Co-Authors: Deepti JainAbstract:Two simple, accurate and precise methods for simultaneous estimation of Atorvastatin Calcium and amlodipine besylate in combined dosage form have been described. First method employs formation and solving of simultaneous equations using 245 nm and 363 nm as two analytical wavelengths. Second is dual wavelength method, which uses the difference of absorbance value at 259.9 nm and 354 nm for estimation of Atorvastatin Calcium and absorbance at 363 nm for amlodipine besylate. Fifty percent methanol was used as solvent, in which Atorvastatin Calcium and amlodipine besylate shows linearity in the range of 0-40 µg/ml and 0-20 µg/ml, respectively. Standard deviation was <1.5 in the assay of tablets. Methods were validated as per ICH norms and accuracy, precision, repeatability and robustness was found to be with in the acceptable limit.
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Simultaneous spectrophotometric determination of Atorvastatin Calcium and amlodipine besylate in tablets
Indian Journal of Pharmaceutical Sciences, 2006Co-Authors: Khan, Deepti JainAbstract:Two simple, accurate and precise methods for simultaneous estimation of Atorvastatin Calcium and amlodipine besylate in combined dosage form have been described. First method employs formation and solving of simultaneous equations using 245 nm and 363 nm as two analytical wavelengths. Second is dual wavelength method, which uses the difference of absorbance value at 259.9 nm and 354 nm for estimation of Atorvastatin Calcium and absorbance at 363 nm for amlodipine besylate. Fifty percent methanol was used as solvent, in which Atorvastatin Calcium and amlodipine besylate shows linearity in the range of 0-40 µg/ml and 0-20 µg/ml, respectively. Standard deviation was
Sanjay J. Surana - One of the best experts on this subject based on the ideXlab platform.
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Simultaneous Determination of Atorvastatin Calcium and Ezetimibe in Pharmaceutical Dosage Form
2016Co-Authors: Eep S. Sonawane, Atul A. Shirkhedkar, R. A. Fursule, Sanjay J. SuranaAbstract:Two methods are described for the simultaneous determination of Atorvastatin Calcium and Ezetimibe in binary mixture. The first method was based on UV-spectrophotometric determination of two drugs, using simultaneous equation method. It involves absorbance measurement at 232.5 nm (λmax of Ezetimibe) and 246.0 nm (λmax of Atorvastatin Calcium) in methanol; linearity was obtained in the range of 5 – 25 µg.mL-1 for both the drugs. The second method was based on HPLC separation of the two drugs in reverse phase mode using Luna C18 column. Linearity was obtained in the concentration range of 8-22 µg.mL-1 for both the drugs. Both these methods have been successively applied to pharmaceutical formulation and were validated according to ICH guidelines
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Simultaneous densitometric TLC analysis of Atorvastatin Calcium and fenofibrate in the bulk drug and in pharmaceutical formulations
Journal of Planar Chromatography – Modern TLC, 2009Co-Authors: Atul A. Shirkhedkar, Sanjay J. SuranaAbstract:The combination of Atorvastatin Calcium and fenofibrate is used for treatment of atherosclerotic vascular diseases. A simple, rapid, selective, and precise densitometric TLC method for simultaneous analysis of Atorvastatin Calcium and fenofibrate in pharmaceutical dosage forms has been established, and validated in accordance with ICH guidelines. Good resolution of Atorvastatin Calcium ( R F 0.36 ± 0.02) and fenofibrate ( R F value of 0.84 ± 0.02) was achieved on aluminum foil silica gel 60 F 254 plates with toluene-methanol-triethylamine 7:3:0.2 ( v/v ) as mobile phase. Detection and quantification were performed densitometrically at 258 nm. Polynomial regression data for the calibration plots showed there was a good linear relationship between response and amount in the range 100–800 ng per band for Atorvastatin Calcium ( r 2 = 0.9983) and 1000–8000 ng per band for fenofibrate ( r 2 = 0.9982). The method was validated for precision accuracy, ruggedness, and recovery. The limits of detection and quantifi...
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Simultaneous spectrophotometric estimation of Atorvastatin Calcium and ezetimibe in tablets
Indian Journal of Pharmaceutical Sciences, 2007Co-Authors: Sandeep Sonawane, Atul A. Shirkhedkar, R. A. Fursule, Sanjay J. SuranaAbstract:A simple, accurate, economical and reproducible procedure for simultaneous estimation of Atorvastatin Calcium and ezetimibe in their tablet dosage form has been developed using, Q-absorbance equation. The method involves, the absorbance measurement at 235.5 nm (iso-absorptive point) and 246.0 nm (λmax of Atorvastatin Calcium), in methanol. Both the drugs, obey Beer-Lambert's law in the concentration range of 5-25 μg/ml. The results of analysis have been validated statistically and by recovery studies.
Indrajeet D. Gonjari - One of the best experts on this subject based on the ideXlab platform.
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Development and validation of RP-HPLC method for determination of Atorvastatin Calcium and Nicotinic acid in combined tablet dosage form
Journal of Saudi Chemical Society, 2016Co-Authors: Jaiprakash N. Sangshetti, Mohammed Aqeel, Zahid Zaheer, Rana Z. Ahmed, M. H. G. Dehghan, Indrajeet D. GonjariAbstract:Abstract A simple, specific and accurate reverse phase liquid chromatographic method was developed for the simultaneous determination of Atorvastatin Calcium and Nicotinic acid in tablet dosage forms. The analysis has been performed by using Agilent ZORBAX SB-C18 (150 × 4.6 mm, 3.5 u) and mobile phase containing acetonitrile: distilled water (85:15) at pH 4.5 (adjusted with phosphoric acid). The detection was carried out at 261 nm with a flow rate of 1.0 ml/min. The retention times of Atorvastatin Calcium and Nicotinic acid were 6.092 and 3.125 min, respectively. The method was validated according to ICH guidelines. The method was validated for specificity, precision, linearity, accuracy and robustness. The linearity for Atorvastatin Calcium and Nicotinic acid were in the range of 2–12 and 10–80 μg/ml respectively. The recoveries of Atorvastatin Calcium and Nicotinic acid were found to be in the range of 99.031% and 99.744% respectively. The proposed method was validated and successfully applied to the estimation of Atorvastatin Calcium and Nicotinic acid in combined tablet formulation.
T. Sreelakshmi - One of the best experts on this subject based on the ideXlab platform.
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Simultaneous Estimation of Atorvastatin Calcium and Felodipine by UV-Spectrophotometric Method in Formulation
Asian Journal of Research in Chemistry, 2011Co-Authors: K. Rajesh, R. Rajalakshmi, S. Vijayaraj, T. SreelakshmiAbstract:A simple, accurate, precise, sensitive and a highly selective UV spectrophotometric method was developed for the simultaneous estimation of Atorvastatin Calcium and Felodipine. The Estimation of Atorvastatin Calcium was carried out at wavelength 241nm, while Felodipine was estimated as single component at 366.5nm. The method was found to be linear in the range of 2–10μg/ml for each Atorvastatin Calcium and Felodipine. The developed method was validated according to ICH guidelines and it found to be accurate and precise. Thus the proposed method can be successfully applied for simultaneous determination of Atorvastatin Calcium and Felodipine in routine analysis work.
Svn Padma - One of the best experts on this subject based on the ideXlab platform.
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Spectrophotometric Methods for the Determination of Atorvastatin Calcium in Pure and It's Pharmaceutical Dosage Forms
Asian Journal of Research in Chemistry, 2008Co-Authors: P Nagaraju, Pasumarthy, Nv Gopal, V. Srinivas, Svn PadmaAbstract:Three simple, sensitive and cost effective Spectrophotometric methods are developed for the determination of Atorvastatin Calcium in pure and its pharmaceutical formulations. These methods are based on the oxidation of Atorvastatin Calcium by ferric chloride in presence of o-phenanthroline (Method A) or 2, 2′bipyridyl (Method B) or potassium ferricyanide (Method C). The colored complex formed was measured at 509, 520 and 735.5 nm for method A, B and C respectively against the reagent blank prepared in the same manner. The optimum experimental parameters for the color production are selected. Beer's law is valid within a concentration range of 20–100 μg/ml, 30–150μg/ml and 10–50 μg/ml for method A, B and C respectively. The mean percentage recoveries are 99.82, 100.1, 99.86 for method A, B and C respectively. The developed methods are applied for the determination of Atorvastatin Calcium in bulk and its pharmaceutical formulations without any interference from excipients.