The Experts below are selected from a list of 180 Experts worldwide ranked by ideXlab platform
Keith L. Perry - One of the best experts on this subject based on the ideXlab platform.
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Potato Aucuba mosaic virus in Potato in New York State.
Plant disease, 2007Co-Authors: J. Susaimuthu, Bright Agindotan, L. Miller, Keith L. PerryAbstract:Solanum tuberosum cv. Elmer's Blue is one of a number of heritage potato accessions maintained at Cornell University that exhibit virus-like symptoms of stunting and a leaf yellowing or a mottle mosaic. Testing of this cultivar by double-antibody sandwich (DAS)-ELISA revealed that it was infected with Potato virus S (PVS) but none of the other common potato viruses screened for in North American potato certification programs (3). Mechanical inoculation of sap from potato cv. Elmer's Blue onto Nicotiana debneyii, N. megalosiphon, N. occidentalis, and N. tabacum produced a range of yellowing and mosaic symptoms (symptomless on N. tabacum), indicating the presence of a transmissible agent, but all these hosts tested negative for PVS. To identify possible viruses, reverse transcription (RT)-PCR assays involving generic primers for different groups of viruses were performed on the potato and the Nicotiana spp. Degenerate primers specific to members of the genus Potexvirus (4) amplified a 600-bp region from the...
Masamichi Takahashi - One of the best experts on this subject based on the ideXlab platform.
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Exine development in Aucuba japonica Thunberg (Cornaceae)
Review of Palaeobotany and Palynology, 1995Co-Authors: Masamichi TakahashiAbstract:The exine structure and its developmental process in Aucuba japonica (Cornaceae) was investigated with scanning and transmission electron microscopy. At the early tetrad stage, the smooth plasma membrane immediately after meiotic cytokinesis differentiates into protruding and invaginating sites. Probacula initiate on the protruding sites of the plasma membrane. Electron-dense materials are gradually accumulated in the developing probacula. These show a heterogeneous electron density within the callose wall. After dissolution of the callose wall, each baculum further elongates, and a foot layer is formed by accumulation of the lamellated structure. The mature exine consists of clavate bacula, a thick foot layer, and an endexine. The developmental process of the intectate exine in Aucuba is similar to that of tectate exines in other taxa.
Yasuhiro Igarashi - One of the best experts on this subject based on the ideXlab platform.
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Draft Genome Sequence of an Anicemycin Producer, Streptomyces sp. TP-A0648.
Genome announcements, 2017Co-Authors: Hisayuki Komaki, Akira Hosoyama, Akane Kimura, Natsuko Ichikawa, Yasuhiro IgarashiAbstract:We report the draft genome sequence of Streptomyces sp. TP-A0648 isolated from a leaf of Aucuba japonica This strain produces a new tumor cell growth inhibitor designated anicemycin. The genome harbors at least 12 biosynthetic gene clusters for polyketides and nonribosomal peptides, suggesting the potential to produce diverse secondary metabolites.
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Anicemycin, a New Inhibitor of Anchorage-independent Growth of Tumor Cells from Streptomyces sp. TP-A0648
The Journal of Antibiotics, 2005Co-Authors: Yasuhiro Igarashi, Koushirou Ootsu, Hiroyasu Onaka, Tsuyoshi Fujita, Yoshimasa Uehara, Tamotsu FurumaiAbstract:The anchorage-independence of cells is closely related to their tumorigenicity. In the screening of inhibitors of anchorage-independent growth of tumor cells, anicemycin was isolated from the fermentation broth of an actinomycete strain TP-A0648. The producing strain was isolated from a leaf of Aucuba japonica collected in Toyama, Japan and identified as Streptomyces sp. based on the taxonomic data. The structure of anicemycin was elucidated as a new analog of spicamycin by NMR and MS analysis. Anicemycin inhibited the anchorage-independent growth of the human ovary cancer SKOV-3 cells with an IC_50 of 0.015 µM about three times more potently than their anchorage-dependent growth.
J. Susaimuthu - One of the best experts on this subject based on the ideXlab platform.
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Potato Aucuba mosaic virus in Potato in New York State.
Plant disease, 2007Co-Authors: J. Susaimuthu, Bright Agindotan, L. Miller, Keith L. PerryAbstract:Solanum tuberosum cv. Elmer's Blue is one of a number of heritage potato accessions maintained at Cornell University that exhibit virus-like symptoms of stunting and a leaf yellowing or a mottle mosaic. Testing of this cultivar by double-antibody sandwich (DAS)-ELISA revealed that it was infected with Potato virus S (PVS) but none of the other common potato viruses screened for in North American potato certification programs (3). Mechanical inoculation of sap from potato cv. Elmer's Blue onto Nicotiana debneyii, N. megalosiphon, N. occidentalis, and N. tabacum produced a range of yellowing and mosaic symptoms (symptomless on N. tabacum), indicating the presence of a transmissible agent, but all these hosts tested negative for PVS. To identify possible viruses, reverse transcription (RT)-PCR assays involving generic primers for different groups of viruses were performed on the potato and the Nicotiana spp. Degenerate primers specific to members of the genus Potexvirus (4) amplified a 600-bp region from the...
D Leclerc - One of the best experts on this subject based on the ideXlab platform.
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the entire nucleotide sequence and genomic organization of potato Aucuba mosaic potexvirus
Archives of Virology, 1994Co-Authors: D Leclerc, B Leung, M G AbouhaidarAbstract:The entire nucleotide sequence (7057 nucleotides) of cDNA clones of potato Aucuba mosaic virus (PAMV) was determined. The genome contains five major putative open reading frames (ORFs), designated from the 5′ terminus as encoding putative proteins with Mr of 187 379 (187 K), 26 326 (26 K), 12 101 (12 K), 8 183 (8 K) and 27 114 (27 K). The genomic organization of PAMV is essentially the same as that of several other potexviruses. The sizes and composition of the proteins encoded by the ORFs are generally similar to those found in other potexviruses. The genome and the capsid protein gene of PAMV, are the largest among the potexviruses sequenced to date.
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Biotinylated DNA probes for detecting virus Y and Aucuba mosaic virus in leaves and dormant tubers of potato
Potato Research, 1992Co-Authors: D Leclerc, M. Eweida, R. P. Singh, Mounir G. AbouhaidarAbstract:Detection of potato virus Y (PVY) in dormant potato tubers using the enzyme-linked immunosorbent assay (ELISA) has been reported not to be accurate and reliable and it requires breaking of the dormancy of tubers prior to testing. We describe a simple, practical and highly sensitive hybridization method based on the use of biotinylated DNA probes for detecting PVY and potato Aucuba mosaic virus (PAMV) in dilutions made of crude extracts of infected potato leaves and dormant tubers. As little as 50 fg of RNA can be detected by this method, and the probes are highly specific for their targets, even in crude plant extracts. The presence of one virus did not interfere with the detection of the other.
