Auditory Ossicle

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Kun Lv - One of the best experts on this subject based on the ideXlab platform.

  • a novel Auditory Ossicles membrane and the development of conductive hearing loss in dmp1 null mice
    Bone, 2017
    Co-Authors: Kun Lv, Haiyang Huang, Xing Yi, Mark E Chertoff, Chaoyuan Li, Baozhi Yuan, Robert J Hinton, Jian Q Feng
    Abstract:

    Abstract Genetic mouse models are widely used for understanding human diseases but we know much less about the anatomical structure of the Auditory Ossicles in the mouse than we do about human Ossicles. Furthermore, current studies have mainly focused on disease conditions such as osteomalacia and rickets in patients with hypophosphatemia rickets, although the reason that these patients develop late-onset hearing loss is unknown. In this study, we first analyzed Dmp1 lac Z knock-in Auditory Ossicles (in which the blue reporter is used to trace DMP1 expression in osteocytes) using X-gal staining and discovered a novel bony membrane surrounding the mouse malleus. This finding was further confirmed by 3-D micro-CT, X-ray, and alizarin red stained images. We speculate that this unique structure amplifies and facilitates sound wave transmissions in two ways: increasing the contact surface between the eardrum and malleus and accelerating the sound transmission due to its mineral content. Next, we documented a progressive deterioration in the Dmp1 -null Auditory Ossicle structures using multiple imaging techniques. The Auditory brainstem response test demonstrated a conductive hearing loss in the adult Dmp1 -null mice. This finding may help to explain in part why patients with DMP1 mutations develop late-onset hearing loss, and supports the critical role of DMP1 in maintaining the integrity of the Auditory Ossicles and its bony membrane.

Jian Q Feng - One of the best experts on this subject based on the ideXlab platform.

  • a novel Auditory Ossicles membrane and the development of conductive hearing loss in dmp1 null mice
    Bone, 2017
    Co-Authors: Kun Lv, Haiyang Huang, Xing Yi, Mark E Chertoff, Chaoyuan Li, Baozhi Yuan, Robert J Hinton, Jian Q Feng
    Abstract:

    Abstract Genetic mouse models are widely used for understanding human diseases but we know much less about the anatomical structure of the Auditory Ossicles in the mouse than we do about human Ossicles. Furthermore, current studies have mainly focused on disease conditions such as osteomalacia and rickets in patients with hypophosphatemia rickets, although the reason that these patients develop late-onset hearing loss is unknown. In this study, we first analyzed Dmp1 lac Z knock-in Auditory Ossicles (in which the blue reporter is used to trace DMP1 expression in osteocytes) using X-gal staining and discovered a novel bony membrane surrounding the mouse malleus. This finding was further confirmed by 3-D micro-CT, X-ray, and alizarin red stained images. We speculate that this unique structure amplifies and facilitates sound wave transmissions in two ways: increasing the contact surface between the eardrum and malleus and accelerating the sound transmission due to its mineral content. Next, we documented a progressive deterioration in the Dmp1 -null Auditory Ossicle structures using multiple imaging techniques. The Auditory brainstem response test demonstrated a conductive hearing loss in the adult Dmp1 -null mice. This finding may help to explain in part why patients with DMP1 mutations develop late-onset hearing loss, and supports the critical role of DMP1 in maintaining the integrity of the Auditory Ossicles and its bony membrane.

Katsuichiro Ohsaki - One of the best experts on this subject based on the ideXlab platform.

  • Histological reaction of Auditory bulla bone to synthetic Auditory Ossicle (Apaceram) in rats.
    The journal of medical investigation : JMI, 2000
    Co-Authors: Y H Tran, Katsuichiro Ohsaki, M Yokozeki, K Moriyama
    Abstract:

    To investigate the biocompatibility of a synthetic Auditory Ossicle to host bone, small thin Apaceram disks composed of dense hydroxyapatite were implanted under the periosteum of the left Auditory bulla in 32 rats for periods ranging from 1 day to 270 days. A sham operation performed on 10 rats served as a control. Decalcified histological sections stained with hematoxylin and eosin were observed using light microscopy. The experiment showed: 1) a time-dependent mature fibrous connective tissue surrounding the Apaceram disk, 2) no evidence of inflammatory reaction caused by the implant from 90 days after implantation until the end of the experiment, 3) no evidence of osteolysis by osteoclasts caused by the implant, and 4) direct contact of bone to the implant on the bone-disk interface at 180 and 270 days after implantation. The findings suggest that Apaceram has a high degree of implant biocompatibility, making it a satisfactory substitute biomaterial for otological reconstructive surgeries.

  • long term observation of subcutaneous tissue reaction to synthetic Auditory Ossicle bioceram in rats
    The Journal of Medical Investigation, 1999
    Co-Authors: Chun-sheng Zhu, Katsuichiro Ohsaki, Yen Hai Tran, Yasuo Ohba, Keiji Moriyama
    Abstract:

    To evaluate biocompatibility to tissue in long-term implantation, Bioceram® discs made of aluminum oxide (Al2O3) were implanted subcutaneously within the interscapular region of 64 rats for six to 20 months. Histological sections stained with haematoxylin and eosin (H&E) and the surface of the implant material were observed using light micros-copy. Different cell types and the thickness of fibrous capsules surrounding the implants were examined quantitatively by light microscopy. Small numbers of macrophages (2.8±0.7%) and lymphocytes (2.7±0.9%) were observed at six months after implantation, gradually decreasing to zero at 16, 18 and 20 months. Neither neutrophils nor foreign body giant cells were seen in any specimens. The thickness of fibrous capsules surrounding the implants was closely related to the shape of the implant, but there was no significant change between six and 20 months after implantation. No change in Bioceram® surfaces were observed under stereoscopic microscopy from six to20months after implantation. The study results indicate that Bioceram® is a satisfactory biocompatible material for reconstructive surgery from the viewpoint of long-term tissue response. Present results of experiments with Bioceram® are also compared to previous results with Apaceram® and different tissue responses of the two materials are discussed.

  • Subcutaneous inflammatory reaction to a synthetic Auditory Ossicle (Bioceram) in rats.
    Acta Oto-Laryngologica, 1999
    Co-Authors: Katsuichiro Ohsaki, Kunio, Chun-sheng Zhu, Yasuhiko Yamashita, Satoru Tenshin, Teruko Takano-yamamoto
    Abstract:

    Cellular response and inflammatory reaction to synthetic Auditory Ossicle (Bioceram®) made from aluminium oxide are investigated. Local inflammatory effects are important in wound healing and in determining biocompatibility of an implant, necessitating the study of biologic effects of implants, especially inflammation and fibrous capsule formation. Bioceram® discs were implanted subcutaneously in the interscapular region of rats for various periods of time, ranging from 1 day to 300 days. Histological sections 6

  • A subcutaneous tissue reaction in the early stage to a synthetic Auditory Ossicle (Bioceram) in rats.
    The journal of medical investigation : JMI, 1998
    Co-Authors: Katsuichiro Ohsaki, Satoru Tenshin, H Matsuoka, T Yamamoto
    Abstract:

    The discs of synthetic Auditory Ossicle (Bioceram), which are composed of aluminium oxide (Al2O3), were implanted subcutaneously in the interscapular region of 16 rats. The implanted specimens were removed at 1, 3, 7 and 14 days after implantation. The decalcified 6 microns thick sections were stained with H.E. and cell types around the implants were counted microscopically. We found that an acute inflammatory reaction occurred at one day, in which macrophages and neutrophiles predominated, and almost disappeared at about 7 days after implantation. Fibrosis began to be observed at 3 days. During this early stage, foreign body giant cells were found in only one specimen at 3 days. These findings, in comparison with those in the controls, showed that the chemical irritation of Bioceram to the subcutaneous tissue is slight, although the physical and/or chemical irritation of Bioceram lasts continuously and induces fibrosis around the bioimplant. The results so far suggest that Bioceram seems to be a satisfactorily biocompatible material, at least within the extent of 2 weeks.

  • long term observation of subcutaneous tissue reaction to synthetic Auditory Ossicle apaceram in rats
    Journal of Laryngology and Otology, 1997
    Co-Authors: Katsuichiro Ohsaki, Satoru Tenshin, Yoko Nobuto, Teruko Takanoyamamoto
    Abstract:

    The present study evaluates histological characteristics of the soft tissue response to long-term implantation of Apaceram® discs composed of dense hydroxyapatite in rats. Discs were implanted into the subcutaneous tissue of 76 rats for six to 20 months. Decalcified histological sections stained with haematoxylin and eosin (H & E) and Mallory's azan were examined. Different cell types surrounding implants were counted. The greatest proportion of macrophages was found at six months (13.5 per cent). This proportion gradually decreased to four per cent at 20 months. Small numbers of lymphocytes and foreign body giant cells were observed in every group, but neither neutrophils nor osteogenesis were observed in any specimens. Results of the present study and previous related studies indicate that despite reappearance of a small number of macrophages six months after implantation, Apaceram® is useful for reconstructive surgery.

Masa-oki Yamada - One of the best experts on this subject based on the ideXlab platform.

  • Age-independent constancy of mineral contents in human vertebra and Auditory Ossicle
    Biological Trace Element Research, 1997
    Co-Authors: Yoshiyuki Tohno, Setsuko Tohno, Masako Utsumi, Takeshi Minami, Masayo Ichii, Yuko Okazaki, Fumio Nishiwaki, Yumi Moriwake, Takashi Naganuma, Masa-oki Yamada
    Abstract:

    To elucidate age-related changes of mineral contents in human bones, element contents of human vertebrae and Auditory Ossicles were determined by inductively coupled plasma atomic-emission spectrometry. The cervical, thoracic, and lumbar vertebrae were removed from 12 vertebral columns. The mallei of Auditory Ossicle were removed from 27 cadavers. It was found that average relative contents (RCs) of calcium and phosphorus in cervical, thoracic, and lumbar vertebrae remained almost constant within ages ranging from 46 to 99 y. In addition, it was found that the RCs of calcium and phosphorus in men’s and women’s mallei remained constant within ages ranging from 40 to 98 yr. These results support the view that there is no significant agedependent change of mineral contents in human bones.

  • Age-independent constancy of mineral contents in human vertebra and Auditory Ossicle.
    Biological Trace Element Research, 1997
    Co-Authors: Yoshiyuki Tohno, Setsuko Tohno, Masako Utsumi, Takeshi Minami, Masayo Ichii, Yuko Okazaki, Fumio Nishiwaki, Yumi Moriwake, Takashi Naganuma, Masa-oki Yamada
    Abstract:

    To elucidate age-related changes of mineral contents in human bones, element contents of human vertebrae and Auditory Ossicles were determined by inductively coupled plasma atomic-emission spectrometry. The cervical, thoracic, and lumbar vertebrae were removed from 12 vertebral columns. The mallei of Auditory Ossicle were removed from 27 cadavers.

Mark E Chertoff - One of the best experts on this subject based on the ideXlab platform.

  • a novel Auditory Ossicles membrane and the development of conductive hearing loss in dmp1 null mice
    Bone, 2017
    Co-Authors: Kun Lv, Haiyang Huang, Xing Yi, Mark E Chertoff, Chaoyuan Li, Baozhi Yuan, Robert J Hinton, Jian Q Feng
    Abstract:

    Abstract Genetic mouse models are widely used for understanding human diseases but we know much less about the anatomical structure of the Auditory Ossicles in the mouse than we do about human Ossicles. Furthermore, current studies have mainly focused on disease conditions such as osteomalacia and rickets in patients with hypophosphatemia rickets, although the reason that these patients develop late-onset hearing loss is unknown. In this study, we first analyzed Dmp1 lac Z knock-in Auditory Ossicles (in which the blue reporter is used to trace DMP1 expression in osteocytes) using X-gal staining and discovered a novel bony membrane surrounding the mouse malleus. This finding was further confirmed by 3-D micro-CT, X-ray, and alizarin red stained images. We speculate that this unique structure amplifies and facilitates sound wave transmissions in two ways: increasing the contact surface between the eardrum and malleus and accelerating the sound transmission due to its mineral content. Next, we documented a progressive deterioration in the Dmp1 -null Auditory Ossicle structures using multiple imaging techniques. The Auditory brainstem response test demonstrated a conductive hearing loss in the adult Dmp1 -null mice. This finding may help to explain in part why patients with DMP1 mutations develop late-onset hearing loss, and supports the critical role of DMP1 in maintaining the integrity of the Auditory Ossicles and its bony membrane.