The Experts below are selected from a list of 174 Experts worldwide ranked by ideXlab platform
Jason L. Rasgon - One of the best experts on this subject based on the ideXlab platform.
-
Vitellogenin gene expression in autogenous Culex tarsalis.
Insect molecular biology, 2010Co-Authors: Katie N. Provost-javier, Song Chen, Jason L. RasgonAbstract:Autogeny, the ability of a mosquito to mature an initial batch of eggs without blood feeding, is an alternative reproductive strategy with important implications for vector-borne disease transmission. Regulation of the major yolk protein (vitellogenin; Vg) genes during bloodmeal-induced oogenesis is well studied, but little is known about regulation of vitellogenesis in autogenous mosquitoes. We characterized the expression of four vitellogenin genes (Vg1a, Vg1b, Vg2a and Vg2b) in an autogenous strain of the West Nile Virus vector, Culex tarsalis. All vitellogenin genes were expressed during autogenous reproduction and following a bloodmeal, although the intensity and duration of expression varied amongst genes. Quantitative PCR analysis of vitellogenin transcription during Autogeny revealed a similar temporal pattern to known vitellogenin expression profiles in anautogenous Aedes aegypti. Vitellogenin transcript, primarily produced from the Vg1b gene, was also detected in the larval and pupal stages of development, but no detectable vitellogenin protein was produced during this time period.
-
Using fluorescently labelled M13-tailed primers to isolate 45 novel microsatellite loci from the arboviral vector Culex tarsalis.
Medical and veterinary entomology, 2007Co-Authors: Meera Venkatesan, Mary Claire Hauer, Jason L. RasgonAbstract:Culex tarsalis Coquillett (Diptera: Culicidae) is a highly efficient arbovirus vector. Spatial and temporal heterogeneity have been observed in Cx tarsalis for phenotypic traits including Autogeny, virus susceptibility and host preference. Genetic differences between populations may in part explain these observations. Using the M13-tailed primer method, we identified 45 novel polymorphic microsatellite markers from microsatellite-enriched Cx tarsalis genomic libraries. The M13-tailed primer method was more efficient in identifying useful loci than traditional screening by acrylamide gel electrophoresis. These markers will be useful for investigating genetic questions in this important vector mosquito.
-
Polymorphic microsatellite loci from the West Nile virus vector Culex tarsalis
Molecular Ecology Notes, 2006Co-Authors: Jason L. Rasgon, Meera Venkatesan, Catherine J. Westbrook, Mary Claire HauerAbstract:Since its introduction in 1999, West Nile virus (WNV) has spread across North America. Culex tarsalis is a highly efficient WNV vector species. Many traits such as virus susceptibility, Autogeny and host preference vary geographically and temporally in C. tarsalis. Culex tarsalis genomic libraries were developed and were highly enriched for microsatellite inserts (42–96%). We identified 12 loci that were polymorphic in wild C. tarsalis populations. These microsatellites are the first DNA-based genetic markers developed for C. tarsalis and will be useful for investigating population structure and constructing genetic maps in this mosquito.
David W Severson - One of the best experts on this subject based on the ideXlab platform.
-
Quantitative trait loci determining Autogeny and body size in the Asian tiger mosquito (Aedes albopictus)
Heredity, 2008Co-Authors: Akio Mori, William C Black, J Romero-severson, David W SeversonAbstract:The majority of mosquito species require a blood meal to stimulate vitellogenesis and subsequent oviposition (anAutogeny), but some autogenous individuals complete their first ovarian cycle without a blood meal. Autogeny may be facultative or obligatory. In this study, we selected for an autogenous strain in the Asian tiger mosquito Aedes albopictus and examined an F_1 intercross population for quantitative trait loci (QTL) determining the Autogeny trait as well as wing length as a proxy for body size. Using composite interval mapping, we identified four QTL for each trait and observed considerable overlap in genome positions between each QTL for Autogeny (follicle size) and wing length. Most QTL were minor in magnitude, individually explaining
-
quantitative trait loci determining Autogeny and body size in the asian tiger mosquito aedes albopictus
Heredity, 2008Co-Authors: Akio Mori, Jeanne Romeroseverson, William C Black, David W SeversonAbstract:The majority of mosquito species require a blood meal to stimulate vitellogenesis and subsequent oviposition (anAutogeny), but some autogenous individuals complete their first ovarian cycle without a blood meal. Autogeny may be facultative or obligatory. In this study, we selected for an autogenous strain in the Asian tiger mosquito Aedes albopictus and examined an F1 intercross population for quantitative trait loci (QTL) determining the Autogeny trait as well as wing length as a proxy for body size. Using composite interval mapping, we identified four QTL for each trait and observed considerable overlap in genome positions between each QTL for Autogeny (follicle size) and wing length. Most QTL were minor in magnitude, individually explaining <10% of the phenotypic variation. Alleles from the autogenous parent generally showed a dominance or overdominance effect on both phenotypes. Strong genetic and phenotypic correlations indicate that Autogeny and wing length are determined by up to four clusters of tightly linked genes or the potential pleiotropic effects of single genes. Although females from the autogenous strain produced approximately fivefold more eggs following a blood meal than through Autogeny, we suggest that the maintenance of alleles for Autogeny in natural populations is likely due to balancing selection. Autogeny should be favored under conditions of limited host availability for blood feeding or increased defensive behavior by the host and adequate larval nutrition. Correlation between Autogeny and body size may reflect an increased ability for larger females to accumulate sufficient nutrient reserves to support oogenesis without the requirement for a blood meal.
Akio Mori - One of the best experts on this subject based on the ideXlab platform.
-
Quantitative trait loci determining Autogeny and body size in the Asian tiger mosquito (Aedes albopictus)
Heredity, 2008Co-Authors: Akio Mori, William C Black, J Romero-severson, David W SeversonAbstract:The majority of mosquito species require a blood meal to stimulate vitellogenesis and subsequent oviposition (anAutogeny), but some autogenous individuals complete their first ovarian cycle without a blood meal. Autogeny may be facultative or obligatory. In this study, we selected for an autogenous strain in the Asian tiger mosquito Aedes albopictus and examined an F_1 intercross population for quantitative trait loci (QTL) determining the Autogeny trait as well as wing length as a proxy for body size. Using composite interval mapping, we identified four QTL for each trait and observed considerable overlap in genome positions between each QTL for Autogeny (follicle size) and wing length. Most QTL were minor in magnitude, individually explaining
-
quantitative trait loci determining Autogeny and body size in the asian tiger mosquito aedes albopictus
Heredity, 2008Co-Authors: Akio Mori, Jeanne Romeroseverson, William C Black, David W SeversonAbstract:The majority of mosquito species require a blood meal to stimulate vitellogenesis and subsequent oviposition (anAutogeny), but some autogenous individuals complete their first ovarian cycle without a blood meal. Autogeny may be facultative or obligatory. In this study, we selected for an autogenous strain in the Asian tiger mosquito Aedes albopictus and examined an F1 intercross population for quantitative trait loci (QTL) determining the Autogeny trait as well as wing length as a proxy for body size. Using composite interval mapping, we identified four QTL for each trait and observed considerable overlap in genome positions between each QTL for Autogeny (follicle size) and wing length. Most QTL were minor in magnitude, individually explaining <10% of the phenotypic variation. Alleles from the autogenous parent generally showed a dominance or overdominance effect on both phenotypes. Strong genetic and phenotypic correlations indicate that Autogeny and wing length are determined by up to four clusters of tightly linked genes or the potential pleiotropic effects of single genes. Although females from the autogenous strain produced approximately fivefold more eggs following a blood meal than through Autogeny, we suggest that the maintenance of alleles for Autogeny in natural populations is likely due to balancing selection. Autogeny should be favored under conditions of limited host availability for blood feeding or increased defensive behavior by the host and adequate larval nutrition. Correlation between Autogeny and body size may reflect an increased ability for larger females to accumulate sufficient nutrient reserves to support oogenesis without the requirement for a blood meal.
Peter A Ryan - One of the best experts on this subject based on the ideXlab platform.
-
Autogeny in ochlerotatus vigilax diptera culicidae from southeast queensland australia
Journal of Medical Entomology, 2003Co-Authors: Leon E Hugo, Peter A RyanAbstract:Field and laboratory investigations were undertaken to determine the level of expression of Autogeny in the mosquito Ochlerotatus vigilax (Skuse) from southeast Queensland, Australia, and whether there was evidence of seasonal variation. At two field sites in southeast Queensland, Wellington Point and Donnybrook, Autogeny rates were determined on six occasions between January 2001 and January 2002. The Autogeny rate varied between 71 and 100% at Wellington Point and between 63 and 100% at Donnybrook. Autogenous fecundity ranged from 17 to 63 eggs per female at Wellington Point and from 13 to 88 eggs per female at Donnybrook. Positive relationships were found between adult body size (indicated by wing length), Autogeny rate, and fecundity. A laboratory study was conducted to investigate the influence of larval nutrition and adult diet (water versus sucrose) on the expression of Autogeny. The Autogeny rate at a low-diet treatment was between 73 and 90% when sucrose was withheld from females and 100% when sucrose was provided. All high-diet females were autogenous. Autogenous egg development required 80 ± 6 h from emergence at 27°C. We conclude that Autogeny rates are consistently high in Oc. vigilax from the southeast Queensland region.
Mary Claire Hauer - One of the best experts on this subject based on the ideXlab platform.
-
Using fluorescently labelled M13-tailed primers to isolate 45 novel microsatellite loci from the arboviral vector Culex tarsalis.
Medical and veterinary entomology, 2007Co-Authors: Meera Venkatesan, Mary Claire Hauer, Jason L. RasgonAbstract:Culex tarsalis Coquillett (Diptera: Culicidae) is a highly efficient arbovirus vector. Spatial and temporal heterogeneity have been observed in Cx tarsalis for phenotypic traits including Autogeny, virus susceptibility and host preference. Genetic differences between populations may in part explain these observations. Using the M13-tailed primer method, we identified 45 novel polymorphic microsatellite markers from microsatellite-enriched Cx tarsalis genomic libraries. The M13-tailed primer method was more efficient in identifying useful loci than traditional screening by acrylamide gel electrophoresis. These markers will be useful for investigating genetic questions in this important vector mosquito.
-
Polymorphic microsatellite loci from the West Nile virus vector Culex tarsalis
Molecular Ecology Notes, 2006Co-Authors: Jason L. Rasgon, Meera Venkatesan, Catherine J. Westbrook, Mary Claire HauerAbstract:Since its introduction in 1999, West Nile virus (WNV) has spread across North America. Culex tarsalis is a highly efficient WNV vector species. Many traits such as virus susceptibility, Autogeny and host preference vary geographically and temporally in C. tarsalis. Culex tarsalis genomic libraries were developed and were highly enriched for microsatellite inserts (42–96%). We identified 12 loci that were polymorphic in wild C. tarsalis populations. These microsatellites are the first DNA-based genetic markers developed for C. tarsalis and will be useful for investigating population structure and constructing genetic maps in this mosquito.
