The Experts below are selected from a list of 2427 Experts worldwide ranked by ideXlab platform
Qiang Yong - One of the best experts on this subject based on the ideXlab platform.
-
Unrevealing model compounds of soil conditioners impacts on the wheat straw Autohydrolysis efficiency and enzymatic hydrolysis.
Biotechnology for biofuels, 2020Co-Authors: Wei Tang, Chen Huang, Caoxing Huang, Chenhuan Lai, Qiang YongAbstract:Soil-derived exogenous ash (EA) poses a challenge toward lignocellulosic Autohydrolysis due to its buffering capacity. Previous works focusing on this phenomenon have failed to also investigate the role that soluble salts, and organic matter plays in this system. Herein, sodium phosphate and sodium humate were employed as model buffering compounds representing soluble salts and organic matter and dosed into a de-ashed wheat straw (DWS) Autohydrolysis process to show the potential impacts of WS attached soil conditioners on the WS Autohydrolysis efficiency which would further affect the enzymatic digestibility of autohydrolyzed WS. Results showed that with the increasing loadings of sodium phosphate and sodium humate resulted in elevated pH values (from 4.0 to 5.1 and from 4.1 to 4.7, respectively). Meanwhile, the reductions of xylan removal yields from ~ 84.3–61.4% to 72.3–53.0% by loading (1–30 g/L) sodium phosphate and sodium humate during WS Autohydrolysis lead to a significant decrease of cellulose accessibilities which finally lead to a reduction of the enzymatic digestibility of autohydrolyzed WS from ~ 75.4–77.2% to 47.3–57.7%. The existence of different types soil conditioner model compounds results in various component fractions from autohydrolyzed WS in the process of Autohydrolysis. A lack of sufficient xylan removal was found to drive the significant decrease in enzymatic accessibility. The results demonstrated the various effects of two typical tested soil conditioners on WS Autohydrolysis and enzymatic hydrolysis.
-
Unrevealing model compounds of soil conditioners impacts on the wheat straw Autohydrolysis efficiency and enzymatic hydrolysis
2020Co-Authors: Wei Tang, Chen Huang, Caoxing Huang, Chenhuan Lai, Qiang YongAbstract:Abstract Background: Soil-derived exogenous ash (EA) poses a challenge toward lignocellulosics Autohydrolysis due to its buffering capacity. Previous works focusing on this phenomenon have failed to also investigate the role that soluble salts, and organic matter plays in this system. Herein, sodium phosphate and sodium humate were employed as model buffering compounds representing soluble salts and organic matter and dosed into a de-ashed wheat straw (DWS) Autohydrolysis process to show the potential impacts of WS attached soil conditioners on the WS Autohydrolysis efficiency which would further affect the enzymatic digestibility of autohydrolyzed WS.Results: Results showed that with the increasing loadings of sodium phosphate and sodium humate resulted in elevated pH values (from 4.0 to 5.1 and from 4.1 to 4.7, respectively). Meanwhile, the reductions of xylan removal yields from ~84.3-61.4% to 72.3-53.0% by loading (1~30 g/L) sodium phosphate and sodium humate during WS Autohydrolysis lead to a significant decrease of cellulose accessibilities which finally lead to a reduction of the enzymatic digestibility of autohydrolyzed WS from ~75.4-77.2% to 47.3-57.7%.Conclusion: The existence of different types soil conditioner model compounds result in various component fractions from autohydrolyzed WS in the process of Autohydrolysis. A lack of sufficient xylan removal was found to drive the significant decrease in enzymatic accessibility. The results demonstrated the various effects of two typical tested soil conditioners on WS Autohydrolysis and enzymatic hydrolysis.
-
Humic acid-assisted Autohydrolysis of waste wheat straw to sustainably improve enzymatic hydrolysis.
Bioresource technology, 2020Co-Authors: Wei Tang, Chen Huang, Caoxing Huang, Chenhuan Lai, Qiang YongAbstract:Abstract Humic acid (HA), considered the main component of organic matter in the ash of waste wheat straw (WWS), has the potential to improve Autohydrolysis through its function as a surfactant. In this work, a pre-washed WWS (PWWS) was subjected to Autohydrolysis with addition of HA to explore whether its surfactant properties can provide benefit to biorefinery operations. Acquired results showed that HA acted as delignification agent likely due to its surfactant properties. Delignification was more than doubled at the maximum HA dosage (30 g/L) relative to the control, which allowed for enzymatic hydrolysis efficiency to also increase from 64.9% to 81.8%. The pretreated materials were further subjected to analysis structure characterization. The results showed that HA effectively reduced the surface lignin area of PWWS, lowering non-specific adsorption of lignin to enzymes. The Autohydrolysis with HA was an effective technique to improve the subsequent cellulose enzymatic digestion by enhancing the delignification.
-
Enhancing enzymatic digestibility of waste wheat straw by presoaking to reduce the ash-influencing effect on Autohydrolysis.
Biotechnology for biofuels, 2019Co-Authors: Wei Tang, Chen Huang, Caoxing Huang, Chenhuan Lai, Qiang YongAbstract:Background The acid buffering capacity of high free ash in waste wheat straw (WWS) has been revealed to be a significant hindrance on the efficiency of Autohydrolysis pretreatment. Previous researches have mainly relied on washing to eliminate the influence of ash, and the underlying mechanism of the ash influencing was not extensively investigated. Presently, studies have found that cations can destroy the acid buffering capacity of ash through cation exchange. Herein, different cations were applied to presoak WWS with the aim to overcome the negative effects of ash on Autohydrolysis efficiency, further improving its enzymatic digestibility.
-
The effects of exogenous ash on the Autohydrolysis and enzymatic hydrolysis of wheat straw.
Bioresource technology, 2019Co-Authors: Wei Tang, Chen Huang, Caoxing Huang, Chenhuan Lai, Qiang YongAbstract:The effects of exogenous ash (EA) from harvest wheat straw and its internal components on wheat straw Autohydrolysis efficiency and subsequent enzymatic hydrolysis were investigated. Results showed that when EA and its insoluble mineral components were included in the Autohydrolysis, the enzymatic efficiencies of pretreated residues were significantly reduced from 84.9% to 66.3% and 58.4%, respectively. This was found to be largely attributable to the buffering of free H+ in the pretreatment medium which took place due to the ash. Specifically, the insoluble mineral fraction of said ash exerted strongest buffering capacity in EA. Furthermore, this decrease was found to linearly correlate with decreases to substrate enzymatic accessibility and hydrophobicity. These results demonstrate that the penalties of ash upon Autohydrolysis are borne of specific fractions comprising the ash, making the case for ash removal processes or supplementation of processes with additives that will counter the negative effects of ash.
Hasan Jameel - One of the best experts on this subject based on the ideXlab platform.
-
Two-stage Autohydrolysis and mechanical treatment to maximize sugar recovery from sweet sorghum bagasse
Bioresource technology, 2018Co-Authors: Bon-wook Koo, Hasan Jameel, Junyeong Park, Ronalds Gonzalez, Sunkyu ParkAbstract:Abstract Modified Autohydrolysis combined with mechanical refining has been suggested to recover free sugars from sweet sorghum bagasse and facilitates enzyme access to cellulose in bagasse for enhancing its conversion to fermentable sugars. The amount of total available sugars in sweet sorghum bagasse was found to be 76.1% and this value was used to evaluate the efficiency of the process suggested. Total sugar recovery was achieved up to 68.1% through the single-stage Autohydrolysis at 170 °C for 60 min, followed by mechanical refining and enzymatic hydrolysis; however, the sugar recovery through partial degradation of free sugars induced by high-temperature Autohydrolysis was lower than expected. A modified two-stage Autohydrolysis was suggested to prevent sugar degradation and the total sugar recovery using this process reached 83.9% of total available sugars in sweet sorghum bagasse.
-
effect of the two stage Autohydrolysis of hardwood on the enzymatic saccharification and subsequent fermentation with an efficient xylose utilizing saccharomyces cerevisiae
Bioresources, 2016Co-Authors: Junyeong Park, Hasan Jameel, Ziyu Wang, Sunkyu ParkAbstract:To effectively utilize sugars during the fermentation process, it is important to develop a process that can minimize the generation of inhibiting compounds such as furans and acids, and a robust micro-organism that can co-ferment both glucose and xylose into products. In this study, the feasibility of efficient ethanol production was investigated using a combination of two approaches: two-stage Autohydrolysis of biomass and fermentation using an engineered Saccharomyces cerevisiae to produce ethanol. When the hardwood chips were autohydrolyzed at 140 °C, followed by the second treatment at 180 °C, a higher yield of sugar conversion and fewer inhibitory effects on subsequent fermentation were achieved compared with the results from single-stage Autohydrolysis. A higher overall yield of ethanol resulted by using an engineered yeast strain, SR8. This observation suggests the possibility of the feasible combination of two-stage Autohydrolysis and the recombinant yeast.
-
Production of fermentable sugars from sugarcane bagasse by enzymatic hydrolysis after Autohydrolysis and mechanical refining.
Bioresource technology, 2015Co-Authors: Larisse Aparecida Ribas Batalha, Jorge Luiz Colodette, Hasan Jameel, Qiang Han, Hou-min Chang, Fernando José Borges GomesAbstract:The Autohydrolysis process has been considered a simple, low-cost and environmental friendly technology for generation of sugars from biomass. In order to improve accessibility of enzymes during enzymatic hydrolysis as well as to allow the recovery of hemicellulose in the filtrate, the sugarcane bagasse was pretreated using Autohydrolysis followed by a mechanical refining process. The Autohydrolysis was carried out in three different conditions. Autohydrolysis at 190°C for 10min provided the highest overall sugar (19.2/100g raw bagasse) in prehydrolyzate. The enzymatic hydrolysis step was performed for all the post-treated solids with and without refining at enzyme loadings of 5 and 10FPU/g for 96h. A total of 84.4% of sugar can be recovered from sugarcane bagasse at 180°C for 20min with 5 FPU/g enzyme charge. The economic analysis for the proposed method showed that the bioethanol production can have a financial return larger than 12%.
-
Autohydrolysis pretreatment of waste wheat straw for cellulosic ethanol production in a co-located straw pulp mill.
Applied biochemistry and biotechnology, 2014Co-Authors: Qiang Han, Hasan Jameel, Hou-min Chang, Yanbin Jin, Richard Phillips, Sunkyu ParkAbstract:Waste wheat straw (WWS) is the waste product from feedstock preparation process in a straw pulp mill. It has a significant annual production rate and no commercial value has been explored on this material. In this study, waste wheat straw was pretreated using an Autohydrolysis process followed by mechanical refining, and the pretreated materials were further enzymatically hydrolyzed to evaluate the total sugar recovery for bioethanol production. Results show that Autohydrolysis at 170 °C for 40 min followed by 6000 revolution PFI refining provided the best result in this study, where a total sugar recovery (total sugars in Autohydrolysis filtrate and enzymatic hydrolyzate over total carbohydrates on raw WWS) of 70 % at 4 filter paper unit per oven dry gram (FPU/OD g) substrate enzyme charge could be obtained. The economic evaluation of this biorefinery process indicates that cellulosic ethanol production from Autohydrolysis of WWS is a very profitable business, with 28.4 % of internal rate of return can be achieved based on current ethanol wholesale price in China.
-
Acid-catalyzed Autohydrolysis of wheat straw to improve sugar recovery.
Bioresource Technology, 2014Co-Authors: Murat Ertas, Hasan JameelAbstract:Abstract A comparison study of Autohydrolysis and acid-catalyzed Autohydrolysis of wheat straw was performed to understand the impact of acid addition on overall sugar recovery. Autohydrolysis combined with refining is capable of achieving sugar recoveries in the mid 70s. If the addition of a small amount of acid is capable of increasing the sugar recovery even higher it may be economically attractive. Acetic, sulfuric, hydrochloric and sulfurous acids were selected for acid-catalyzed Autohydrolysis pretreatments. Autohydrolysis with no acid at 190 °C showed the highest total sugar in the prehydrolyzate. Enzymatic hydrolysis was performed for all the post-treated solids with and without refining at enzyme loadings of 4 and 10 FPU/g for 96 h. Acid-catalyzed Autohydrolysis at 190 °C with sulfurous acid showed the highest total sugar recovery of 81.2% at 4 FPU/g enzyme charge compared with 64.3% at 190 °C Autohydrolysis without acid.
Wei Tang - One of the best experts on this subject based on the ideXlab platform.
-
Unrevealing model compounds of soil conditioners impacts on the wheat straw Autohydrolysis efficiency and enzymatic hydrolysis.
Biotechnology for biofuels, 2020Co-Authors: Wei Tang, Chen Huang, Caoxing Huang, Chenhuan Lai, Qiang YongAbstract:Soil-derived exogenous ash (EA) poses a challenge toward lignocellulosic Autohydrolysis due to its buffering capacity. Previous works focusing on this phenomenon have failed to also investigate the role that soluble salts, and organic matter plays in this system. Herein, sodium phosphate and sodium humate were employed as model buffering compounds representing soluble salts and organic matter and dosed into a de-ashed wheat straw (DWS) Autohydrolysis process to show the potential impacts of WS attached soil conditioners on the WS Autohydrolysis efficiency which would further affect the enzymatic digestibility of autohydrolyzed WS. Results showed that with the increasing loadings of sodium phosphate and sodium humate resulted in elevated pH values (from 4.0 to 5.1 and from 4.1 to 4.7, respectively). Meanwhile, the reductions of xylan removal yields from ~ 84.3–61.4% to 72.3–53.0% by loading (1–30 g/L) sodium phosphate and sodium humate during WS Autohydrolysis lead to a significant decrease of cellulose accessibilities which finally lead to a reduction of the enzymatic digestibility of autohydrolyzed WS from ~ 75.4–77.2% to 47.3–57.7%. The existence of different types soil conditioner model compounds results in various component fractions from autohydrolyzed WS in the process of Autohydrolysis. A lack of sufficient xylan removal was found to drive the significant decrease in enzymatic accessibility. The results demonstrated the various effects of two typical tested soil conditioners on WS Autohydrolysis and enzymatic hydrolysis.
-
Unrevealing model compounds of soil conditioners impacts on the wheat straw Autohydrolysis efficiency and enzymatic hydrolysis
2020Co-Authors: Wei Tang, Chen Huang, Caoxing Huang, Chenhuan Lai, Qiang YongAbstract:Abstract Background: Soil-derived exogenous ash (EA) poses a challenge toward lignocellulosics Autohydrolysis due to its buffering capacity. Previous works focusing on this phenomenon have failed to also investigate the role that soluble salts, and organic matter plays in this system. Herein, sodium phosphate and sodium humate were employed as model buffering compounds representing soluble salts and organic matter and dosed into a de-ashed wheat straw (DWS) Autohydrolysis process to show the potential impacts of WS attached soil conditioners on the WS Autohydrolysis efficiency which would further affect the enzymatic digestibility of autohydrolyzed WS.Results: Results showed that with the increasing loadings of sodium phosphate and sodium humate resulted in elevated pH values (from 4.0 to 5.1 and from 4.1 to 4.7, respectively). Meanwhile, the reductions of xylan removal yields from ~84.3-61.4% to 72.3-53.0% by loading (1~30 g/L) sodium phosphate and sodium humate during WS Autohydrolysis lead to a significant decrease of cellulose accessibilities which finally lead to a reduction of the enzymatic digestibility of autohydrolyzed WS from ~75.4-77.2% to 47.3-57.7%.Conclusion: The existence of different types soil conditioner model compounds result in various component fractions from autohydrolyzed WS in the process of Autohydrolysis. A lack of sufficient xylan removal was found to drive the significant decrease in enzymatic accessibility. The results demonstrated the various effects of two typical tested soil conditioners on WS Autohydrolysis and enzymatic hydrolysis.
-
Humic acid-assisted Autohydrolysis of waste wheat straw to sustainably improve enzymatic hydrolysis.
Bioresource technology, 2020Co-Authors: Wei Tang, Chen Huang, Caoxing Huang, Chenhuan Lai, Qiang YongAbstract:Abstract Humic acid (HA), considered the main component of organic matter in the ash of waste wheat straw (WWS), has the potential to improve Autohydrolysis through its function as a surfactant. In this work, a pre-washed WWS (PWWS) was subjected to Autohydrolysis with addition of HA to explore whether its surfactant properties can provide benefit to biorefinery operations. Acquired results showed that HA acted as delignification agent likely due to its surfactant properties. Delignification was more than doubled at the maximum HA dosage (30 g/L) relative to the control, which allowed for enzymatic hydrolysis efficiency to also increase from 64.9% to 81.8%. The pretreated materials were further subjected to analysis structure characterization. The results showed that HA effectively reduced the surface lignin area of PWWS, lowering non-specific adsorption of lignin to enzymes. The Autohydrolysis with HA was an effective technique to improve the subsequent cellulose enzymatic digestion by enhancing the delignification.
-
Enhancing enzymatic digestibility of waste wheat straw by presoaking to reduce the ash-influencing effect on Autohydrolysis.
Biotechnology for biofuels, 2019Co-Authors: Wei Tang, Chen Huang, Caoxing Huang, Chenhuan Lai, Qiang YongAbstract:Background The acid buffering capacity of high free ash in waste wheat straw (WWS) has been revealed to be a significant hindrance on the efficiency of Autohydrolysis pretreatment. Previous researches have mainly relied on washing to eliminate the influence of ash, and the underlying mechanism of the ash influencing was not extensively investigated. Presently, studies have found that cations can destroy the acid buffering capacity of ash through cation exchange. Herein, different cations were applied to presoak WWS with the aim to overcome the negative effects of ash on Autohydrolysis efficiency, further improving its enzymatic digestibility.
-
The effects of exogenous ash on the Autohydrolysis and enzymatic hydrolysis of wheat straw.
Bioresource technology, 2019Co-Authors: Wei Tang, Chen Huang, Caoxing Huang, Chenhuan Lai, Qiang YongAbstract:The effects of exogenous ash (EA) from harvest wheat straw and its internal components on wheat straw Autohydrolysis efficiency and subsequent enzymatic hydrolysis were investigated. Results showed that when EA and its insoluble mineral components were included in the Autohydrolysis, the enzymatic efficiencies of pretreated residues were significantly reduced from 84.9% to 66.3% and 58.4%, respectively. This was found to be largely attributable to the buffering of free H+ in the pretreatment medium which took place due to the ash. Specifically, the insoluble mineral fraction of said ash exerted strongest buffering capacity in EA. Furthermore, this decrease was found to linearly correlate with decreases to substrate enzymatic accessibility and hydrophobicity. These results demonstrate that the penalties of ash upon Autohydrolysis are borne of specific fractions comprising the ash, making the case for ash removal processes or supplementation of processes with additives that will counter the negative effects of ash.
Chen Huang - One of the best experts on this subject based on the ideXlab platform.
-
Unrevealing model compounds of soil conditioners impacts on the wheat straw Autohydrolysis efficiency and enzymatic hydrolysis.
Biotechnology for biofuels, 2020Co-Authors: Wei Tang, Chen Huang, Caoxing Huang, Chenhuan Lai, Qiang YongAbstract:Soil-derived exogenous ash (EA) poses a challenge toward lignocellulosic Autohydrolysis due to its buffering capacity. Previous works focusing on this phenomenon have failed to also investigate the role that soluble salts, and organic matter plays in this system. Herein, sodium phosphate and sodium humate were employed as model buffering compounds representing soluble salts and organic matter and dosed into a de-ashed wheat straw (DWS) Autohydrolysis process to show the potential impacts of WS attached soil conditioners on the WS Autohydrolysis efficiency which would further affect the enzymatic digestibility of autohydrolyzed WS. Results showed that with the increasing loadings of sodium phosphate and sodium humate resulted in elevated pH values (from 4.0 to 5.1 and from 4.1 to 4.7, respectively). Meanwhile, the reductions of xylan removal yields from ~ 84.3–61.4% to 72.3–53.0% by loading (1–30 g/L) sodium phosphate and sodium humate during WS Autohydrolysis lead to a significant decrease of cellulose accessibilities which finally lead to a reduction of the enzymatic digestibility of autohydrolyzed WS from ~ 75.4–77.2% to 47.3–57.7%. The existence of different types soil conditioner model compounds results in various component fractions from autohydrolyzed WS in the process of Autohydrolysis. A lack of sufficient xylan removal was found to drive the significant decrease in enzymatic accessibility. The results demonstrated the various effects of two typical tested soil conditioners on WS Autohydrolysis and enzymatic hydrolysis.
-
Unrevealing model compounds of soil conditioners impacts on the wheat straw Autohydrolysis efficiency and enzymatic hydrolysis
2020Co-Authors: Wei Tang, Chen Huang, Caoxing Huang, Chenhuan Lai, Qiang YongAbstract:Abstract Background: Soil-derived exogenous ash (EA) poses a challenge toward lignocellulosics Autohydrolysis due to its buffering capacity. Previous works focusing on this phenomenon have failed to also investigate the role that soluble salts, and organic matter plays in this system. Herein, sodium phosphate and sodium humate were employed as model buffering compounds representing soluble salts and organic matter and dosed into a de-ashed wheat straw (DWS) Autohydrolysis process to show the potential impacts of WS attached soil conditioners on the WS Autohydrolysis efficiency which would further affect the enzymatic digestibility of autohydrolyzed WS.Results: Results showed that with the increasing loadings of sodium phosphate and sodium humate resulted in elevated pH values (from 4.0 to 5.1 and from 4.1 to 4.7, respectively). Meanwhile, the reductions of xylan removal yields from ~84.3-61.4% to 72.3-53.0% by loading (1~30 g/L) sodium phosphate and sodium humate during WS Autohydrolysis lead to a significant decrease of cellulose accessibilities which finally lead to a reduction of the enzymatic digestibility of autohydrolyzed WS from ~75.4-77.2% to 47.3-57.7%.Conclusion: The existence of different types soil conditioner model compounds result in various component fractions from autohydrolyzed WS in the process of Autohydrolysis. A lack of sufficient xylan removal was found to drive the significant decrease in enzymatic accessibility. The results demonstrated the various effects of two typical tested soil conditioners on WS Autohydrolysis and enzymatic hydrolysis.
-
Humic acid-assisted Autohydrolysis of waste wheat straw to sustainably improve enzymatic hydrolysis.
Bioresource technology, 2020Co-Authors: Wei Tang, Chen Huang, Caoxing Huang, Chenhuan Lai, Qiang YongAbstract:Abstract Humic acid (HA), considered the main component of organic matter in the ash of waste wheat straw (WWS), has the potential to improve Autohydrolysis through its function as a surfactant. In this work, a pre-washed WWS (PWWS) was subjected to Autohydrolysis with addition of HA to explore whether its surfactant properties can provide benefit to biorefinery operations. Acquired results showed that HA acted as delignification agent likely due to its surfactant properties. Delignification was more than doubled at the maximum HA dosage (30 g/L) relative to the control, which allowed for enzymatic hydrolysis efficiency to also increase from 64.9% to 81.8%. The pretreated materials were further subjected to analysis structure characterization. The results showed that HA effectively reduced the surface lignin area of PWWS, lowering non-specific adsorption of lignin to enzymes. The Autohydrolysis with HA was an effective technique to improve the subsequent cellulose enzymatic digestion by enhancing the delignification.
-
Enhancing enzymatic digestibility of waste wheat straw by presoaking to reduce the ash-influencing effect on Autohydrolysis.
Biotechnology for biofuels, 2019Co-Authors: Wei Tang, Chen Huang, Caoxing Huang, Chenhuan Lai, Qiang YongAbstract:Background The acid buffering capacity of high free ash in waste wheat straw (WWS) has been revealed to be a significant hindrance on the efficiency of Autohydrolysis pretreatment. Previous researches have mainly relied on washing to eliminate the influence of ash, and the underlying mechanism of the ash influencing was not extensively investigated. Presently, studies have found that cations can destroy the acid buffering capacity of ash through cation exchange. Herein, different cations were applied to presoak WWS with the aim to overcome the negative effects of ash on Autohydrolysis efficiency, further improving its enzymatic digestibility.
-
The effects of exogenous ash on the Autohydrolysis and enzymatic hydrolysis of wheat straw.
Bioresource technology, 2019Co-Authors: Wei Tang, Chen Huang, Caoxing Huang, Chenhuan Lai, Qiang YongAbstract:The effects of exogenous ash (EA) from harvest wheat straw and its internal components on wheat straw Autohydrolysis efficiency and subsequent enzymatic hydrolysis were investigated. Results showed that when EA and its insoluble mineral components were included in the Autohydrolysis, the enzymatic efficiencies of pretreated residues were significantly reduced from 84.9% to 66.3% and 58.4%, respectively. This was found to be largely attributable to the buffering of free H+ in the pretreatment medium which took place due to the ash. Specifically, the insoluble mineral fraction of said ash exerted strongest buffering capacity in EA. Furthermore, this decrease was found to linearly correlate with decreases to substrate enzymatic accessibility and hydrophobicity. These results demonstrate that the penalties of ash upon Autohydrolysis are borne of specific fractions comprising the ash, making the case for ash removal processes or supplementation of processes with additives that will counter the negative effects of ash.
Chenhuan Lai - One of the best experts on this subject based on the ideXlab platform.
-
Unrevealing model compounds of soil conditioners impacts on the wheat straw Autohydrolysis efficiency and enzymatic hydrolysis.
Biotechnology for biofuels, 2020Co-Authors: Wei Tang, Chen Huang, Caoxing Huang, Chenhuan Lai, Qiang YongAbstract:Soil-derived exogenous ash (EA) poses a challenge toward lignocellulosic Autohydrolysis due to its buffering capacity. Previous works focusing on this phenomenon have failed to also investigate the role that soluble salts, and organic matter plays in this system. Herein, sodium phosphate and sodium humate were employed as model buffering compounds representing soluble salts and organic matter and dosed into a de-ashed wheat straw (DWS) Autohydrolysis process to show the potential impacts of WS attached soil conditioners on the WS Autohydrolysis efficiency which would further affect the enzymatic digestibility of autohydrolyzed WS. Results showed that with the increasing loadings of sodium phosphate and sodium humate resulted in elevated pH values (from 4.0 to 5.1 and from 4.1 to 4.7, respectively). Meanwhile, the reductions of xylan removal yields from ~ 84.3–61.4% to 72.3–53.0% by loading (1–30 g/L) sodium phosphate and sodium humate during WS Autohydrolysis lead to a significant decrease of cellulose accessibilities which finally lead to a reduction of the enzymatic digestibility of autohydrolyzed WS from ~ 75.4–77.2% to 47.3–57.7%. The existence of different types soil conditioner model compounds results in various component fractions from autohydrolyzed WS in the process of Autohydrolysis. A lack of sufficient xylan removal was found to drive the significant decrease in enzymatic accessibility. The results demonstrated the various effects of two typical tested soil conditioners on WS Autohydrolysis and enzymatic hydrolysis.
-
Unrevealing model compounds of soil conditioners impacts on the wheat straw Autohydrolysis efficiency and enzymatic hydrolysis
2020Co-Authors: Wei Tang, Chen Huang, Caoxing Huang, Chenhuan Lai, Qiang YongAbstract:Abstract Background: Soil-derived exogenous ash (EA) poses a challenge toward lignocellulosics Autohydrolysis due to its buffering capacity. Previous works focusing on this phenomenon have failed to also investigate the role that soluble salts, and organic matter plays in this system. Herein, sodium phosphate and sodium humate were employed as model buffering compounds representing soluble salts and organic matter and dosed into a de-ashed wheat straw (DWS) Autohydrolysis process to show the potential impacts of WS attached soil conditioners on the WS Autohydrolysis efficiency which would further affect the enzymatic digestibility of autohydrolyzed WS.Results: Results showed that with the increasing loadings of sodium phosphate and sodium humate resulted in elevated pH values (from 4.0 to 5.1 and from 4.1 to 4.7, respectively). Meanwhile, the reductions of xylan removal yields from ~84.3-61.4% to 72.3-53.0% by loading (1~30 g/L) sodium phosphate and sodium humate during WS Autohydrolysis lead to a significant decrease of cellulose accessibilities which finally lead to a reduction of the enzymatic digestibility of autohydrolyzed WS from ~75.4-77.2% to 47.3-57.7%.Conclusion: The existence of different types soil conditioner model compounds result in various component fractions from autohydrolyzed WS in the process of Autohydrolysis. A lack of sufficient xylan removal was found to drive the significant decrease in enzymatic accessibility. The results demonstrated the various effects of two typical tested soil conditioners on WS Autohydrolysis and enzymatic hydrolysis.
-
Humic acid-assisted Autohydrolysis of waste wheat straw to sustainably improve enzymatic hydrolysis.
Bioresource technology, 2020Co-Authors: Wei Tang, Chen Huang, Caoxing Huang, Chenhuan Lai, Qiang YongAbstract:Abstract Humic acid (HA), considered the main component of organic matter in the ash of waste wheat straw (WWS), has the potential to improve Autohydrolysis through its function as a surfactant. In this work, a pre-washed WWS (PWWS) was subjected to Autohydrolysis with addition of HA to explore whether its surfactant properties can provide benefit to biorefinery operations. Acquired results showed that HA acted as delignification agent likely due to its surfactant properties. Delignification was more than doubled at the maximum HA dosage (30 g/L) relative to the control, which allowed for enzymatic hydrolysis efficiency to also increase from 64.9% to 81.8%. The pretreated materials were further subjected to analysis structure characterization. The results showed that HA effectively reduced the surface lignin area of PWWS, lowering non-specific adsorption of lignin to enzymes. The Autohydrolysis with HA was an effective technique to improve the subsequent cellulose enzymatic digestion by enhancing the delignification.
-
Enhancing enzymatic digestibility of waste wheat straw by presoaking to reduce the ash-influencing effect on Autohydrolysis.
Biotechnology for biofuels, 2019Co-Authors: Wei Tang, Chen Huang, Caoxing Huang, Chenhuan Lai, Qiang YongAbstract:Background The acid buffering capacity of high free ash in waste wheat straw (WWS) has been revealed to be a significant hindrance on the efficiency of Autohydrolysis pretreatment. Previous researches have mainly relied on washing to eliminate the influence of ash, and the underlying mechanism of the ash influencing was not extensively investigated. Presently, studies have found that cations can destroy the acid buffering capacity of ash through cation exchange. Herein, different cations were applied to presoak WWS with the aim to overcome the negative effects of ash on Autohydrolysis efficiency, further improving its enzymatic digestibility.
-
The effects of exogenous ash on the Autohydrolysis and enzymatic hydrolysis of wheat straw.
Bioresource technology, 2019Co-Authors: Wei Tang, Chen Huang, Caoxing Huang, Chenhuan Lai, Qiang YongAbstract:The effects of exogenous ash (EA) from harvest wheat straw and its internal components on wheat straw Autohydrolysis efficiency and subsequent enzymatic hydrolysis were investigated. Results showed that when EA and its insoluble mineral components were included in the Autohydrolysis, the enzymatic efficiencies of pretreated residues were significantly reduced from 84.9% to 66.3% and 58.4%, respectively. This was found to be largely attributable to the buffering of free H+ in the pretreatment medium which took place due to the ash. Specifically, the insoluble mineral fraction of said ash exerted strongest buffering capacity in EA. Furthermore, this decrease was found to linearly correlate with decreases to substrate enzymatic accessibility and hydrophobicity. These results demonstrate that the penalties of ash upon Autohydrolysis are borne of specific fractions comprising the ash, making the case for ash removal processes or supplementation of processes with additives that will counter the negative effects of ash.
