The Experts below are selected from a list of 87 Experts worldwide ranked by ideXlab platform
Dabing Zhang - One of the best experts on this subject based on the ideXlab platform.
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genetic characterization of a new astrovirus in goslings suffering from gout
Archives of Virology, 2018Co-Authors: Meiling Jin, Xiaoyan Wang, Ning Liu, Kang Ning, Dabing ZhangAbstract:Since early 2016, the Chinese goose industry has experienced severe outbreaks of gout; however, the etiological factor of the disease is still unclear. Here, we investigated the possible involvement of viral infection in the disease. Using sequence-independent PCR amplification, astrovirus sequences were generated from a gout case. Full-length genomic sequencing and sequence analysis of three goose astrovirus (GoAstV) strains revealed that they belong to a new Avastrovirus most closely related to viruses classified within species Avastrovirus 3. The GoAstV was detected in 16/16 gout cases collected from two provinces, supporting a pathogenic role for the new Avastrovirus.
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complete genome sequence of a novel Avastrovirus in goose
Archives of Virology, 2017Co-Authors: Yuxuan Zhang, Fumin Wang, Ning Liu, Lin Yang, Dabing ZhangAbstract:We report the complete genome sequence of a new Avastrovirus of goose-origin (FLX). The 7299-nt-long genome consisted of three overlapping open reading frames (ORFs) that were in different reading frames. Pairwise comparisons showed that the FLX genome was 59% identical to its closest relatives and that the levels of amino acid identity shared by FLX with other astroviruses did not exceed 54% in ORF1a, 66% in ORF1b, and 50% in ORF2, respectively. Phylogenetic analysis based on the amino acid sequence of the full-length ORF2 demonstrated that FLX was highly divergent from all other Avastroviruses. At the amino acid level the complete capsid region of FLX shared genetic distances of 0.574–0.719 with three official Avastrovirus species, suggesting that it can be classified as a member of a novel species in the genus Avastrovirus.
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genetic characterization of a novel astrovirus in pekin ducks
Infection Genetics and Evolution, 2015Co-Authors: Qinfeng Liao, Fumin Wang, Ning Liu, Xiaoyan Wang, Dabing ZhangAbstract:Three divergent groups of duck astroviruses (DAstVs), namely DAstV-1, DAstV-2 (formerly duck hepatitis virus type 3) and DAstV-3 (isolate CPH), and other Avastroviruses are known to infect domestic ducks. To provide more data regarding the molecular epidemiology of astroviruses in domestic ducks, we examined the prevalence of astroviruses in 136 domestic duck samples collected from four different provinces of China. Nineteen goose samples were also included. Using an astrovirus-specific reverse transcription-PCR assay, two groups of astroviruses were detected from our samples. A group of astroviruses detected from Pekin ducks, Shaoxing ducks and Landes geese were highly similar to the newly discovered DAstV-3. More interestingly, a novel group of Avastroviruses, which we named DAstV-4, was detected in Pekin ducks. Following full-length sequencing and sequence analysis, the variation between DAstV-4 and other Avastroviruses in terms of lengths of genome and internal component was highlighted. Sequence identity and phylogenetic analyses based on the amino acid sequences of the three open reading frames (ORFs) clearly demonstrated that DAstV-4 was highly divergent from all other Avastroviruses. Further analyses showed that DAstV-4 shared low levels of genome identities (50-58%) and high levels of mean amino acid genetic distances in the ORF2 sequences (0.520-0.801) with other Avastroviruses, suggesting DAstV-4 may represent an additional Avastrovirus species although the taxonomic relationship of DAstV-4 to DAstV-3 remains to be resolved. The present works contribute to the understanding of epidemiology, ecology and taxonomy of astroviruses in ducks.
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molecular characterization of a duck hepatitis virus 3 like astrovirus
Veterinary Microbiology, 2014Co-Authors: Ning Liu, Fumin Wang, Jiajian Shi, Lisha Zheng, Xiaoyan Wang, Dabing ZhangAbstract:Using an ORF1b-based astrovirus-specific reverse transcription (RT)-PCR assay, a duck hepatitis virus type 3 (DHV-3)-like astrovirus was detected from four intestinal samples collected from diseased ducks in China. Complete genome sequencing and comparative sequence analysis showed that the four duck astrovirus (DAstV) isolates were closely related and possessed a typical astrovirus genome organization. Genetic analysis of the complete ORF2 region revealed that mean amino acid genetic distances between the DHV-3-like isolates and previously known Avastrovirus species were between 0.579 and 0.721, suggesting that the DHV-3-like isolates could be classified as an additional Avastrovirus species. In the ORF1a and ORF1b regions, however, mean amino acid genetic distances between the DHV-3-like viruses and the turkey astrovirus 2 (TAstV-2)-like isolates were substantially less than those between TAstV-2-like isolates and DAstV/C-NGB-like astroviruses belonging to the same species. Pairwise comparisons and phylogenetic analyses demonstrated that the DHV-3-like isolates were most closely related to TAstV-2-like viruses in ORF1a and ORF1b, while showed highest similarity with the chicken astrovirus (CAstV) 612-like viruses in ORF2. These findings provide evidence that recombination events may have occurred during evolution of the Avastroviruses and support the view that genomic analysis is required for classification of the Avastroviruses.
Ning Liu - One of the best experts on this subject based on the ideXlab platform.
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genetic characterization of a new astrovirus in goslings suffering from gout
Archives of Virology, 2018Co-Authors: Meiling Jin, Xiaoyan Wang, Ning Liu, Kang Ning, Dabing ZhangAbstract:Since early 2016, the Chinese goose industry has experienced severe outbreaks of gout; however, the etiological factor of the disease is still unclear. Here, we investigated the possible involvement of viral infection in the disease. Using sequence-independent PCR amplification, astrovirus sequences were generated from a gout case. Full-length genomic sequencing and sequence analysis of three goose astrovirus (GoAstV) strains revealed that they belong to a new Avastrovirus most closely related to viruses classified within species Avastrovirus 3. The GoAstV was detected in 16/16 gout cases collected from two provinces, supporting a pathogenic role for the new Avastrovirus.
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complete genome sequence of a novel Avastrovirus in goose
Archives of Virology, 2017Co-Authors: Yuxuan Zhang, Fumin Wang, Ning Liu, Lin Yang, Dabing ZhangAbstract:We report the complete genome sequence of a new Avastrovirus of goose-origin (FLX). The 7299-nt-long genome consisted of three overlapping open reading frames (ORFs) that were in different reading frames. Pairwise comparisons showed that the FLX genome was 59% identical to its closest relatives and that the levels of amino acid identity shared by FLX with other astroviruses did not exceed 54% in ORF1a, 66% in ORF1b, and 50% in ORF2, respectively. Phylogenetic analysis based on the amino acid sequence of the full-length ORF2 demonstrated that FLX was highly divergent from all other Avastroviruses. At the amino acid level the complete capsid region of FLX shared genetic distances of 0.574–0.719 with three official Avastrovirus species, suggesting that it can be classified as a member of a novel species in the genus Avastrovirus.
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genetic characterization of a novel astrovirus in pekin ducks
Infection Genetics and Evolution, 2015Co-Authors: Qinfeng Liao, Fumin Wang, Ning Liu, Xiaoyan Wang, Dabing ZhangAbstract:Three divergent groups of duck astroviruses (DAstVs), namely DAstV-1, DAstV-2 (formerly duck hepatitis virus type 3) and DAstV-3 (isolate CPH), and other Avastroviruses are known to infect domestic ducks. To provide more data regarding the molecular epidemiology of astroviruses in domestic ducks, we examined the prevalence of astroviruses in 136 domestic duck samples collected from four different provinces of China. Nineteen goose samples were also included. Using an astrovirus-specific reverse transcription-PCR assay, two groups of astroviruses were detected from our samples. A group of astroviruses detected from Pekin ducks, Shaoxing ducks and Landes geese were highly similar to the newly discovered DAstV-3. More interestingly, a novel group of Avastroviruses, which we named DAstV-4, was detected in Pekin ducks. Following full-length sequencing and sequence analysis, the variation between DAstV-4 and other Avastroviruses in terms of lengths of genome and internal component was highlighted. Sequence identity and phylogenetic analyses based on the amino acid sequences of the three open reading frames (ORFs) clearly demonstrated that DAstV-4 was highly divergent from all other Avastroviruses. Further analyses showed that DAstV-4 shared low levels of genome identities (50-58%) and high levels of mean amino acid genetic distances in the ORF2 sequences (0.520-0.801) with other Avastroviruses, suggesting DAstV-4 may represent an additional Avastrovirus species although the taxonomic relationship of DAstV-4 to DAstV-3 remains to be resolved. The present works contribute to the understanding of epidemiology, ecology and taxonomy of astroviruses in ducks.
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molecular characterization of a duck hepatitis virus 3 like astrovirus
Veterinary Microbiology, 2014Co-Authors: Ning Liu, Fumin Wang, Jiajian Shi, Lisha Zheng, Xiaoyan Wang, Dabing ZhangAbstract:Using an ORF1b-based astrovirus-specific reverse transcription (RT)-PCR assay, a duck hepatitis virus type 3 (DHV-3)-like astrovirus was detected from four intestinal samples collected from diseased ducks in China. Complete genome sequencing and comparative sequence analysis showed that the four duck astrovirus (DAstV) isolates were closely related and possessed a typical astrovirus genome organization. Genetic analysis of the complete ORF2 region revealed that mean amino acid genetic distances between the DHV-3-like isolates and previously known Avastrovirus species were between 0.579 and 0.721, suggesting that the DHV-3-like isolates could be classified as an additional Avastrovirus species. In the ORF1a and ORF1b regions, however, mean amino acid genetic distances between the DHV-3-like viruses and the turkey astrovirus 2 (TAstV-2)-like isolates were substantially less than those between TAstV-2-like isolates and DAstV/C-NGB-like astroviruses belonging to the same species. Pairwise comparisons and phylogenetic analyses demonstrated that the DHV-3-like isolates were most closely related to TAstV-2-like viruses in ORF1a and ORF1b, while showed highest similarity with the chicken astrovirus (CAstV) 612-like viruses in ORF2. These findings provide evidence that recombination events may have occurred during evolution of the Avastroviruses and support the view that genomic analysis is required for classification of the Avastroviruses.
Ákos Boros - One of the best experts on this subject based on the ideXlab platform.
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Identification of a novel astrovirus in domestic sheep in Hungary
Archives of Virology, 2011Co-Authors: Gábor Reuter, Péter Pankovics, Eric Delwart, Ákos BorosAbstract:The family Astroviridae consists of two genera, Avastrovirus and Mamastrovirus, whose members are associated with gastroenteritis in avian and mammalian hosts, respectively. We serendipitously identified a novel ovine astrovirus in a fecal specimen from a domestic sheep (Ovis aries) in Hungary by viral metagenomic analysis. Sequencing of the fragment indicated that it was an ORF1b/ORF2/3′UTR sequence, and it has been submitted to the GenBank database as ovine astrovirus type 2 (OAstV-2/Hungary/2009) with accession number JN592482. The unique sequence characteristics and the phylogenetic position of OAstV-2 suggest that genetically divergent lineages of astroviruses exist in sheep.
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identification of a novel astrovirus in a domestic pig in hungary
Archives of Virology, 2011Co-Authors: Gábor Reuter, Péter Pankovics, Ákos BorosAbstract:The family Astroviridae consists of two genera, Avastrovirus and Mamastrovirus, whose members are associated with gastroenteritis in avian and mammalian hosts, respectively. We serendipitously identified a novel porcine astrovirus in a fecal specimen from a domestic pig (Sus scrofa domestica) in Hungary. Sequencing of a fragment indicated that it was an ORF1b/ORF2/3'UTR sequence, and it has been submitted to the database as porcine astrovirus type 2 (PAstV-2/Hungary/2007) with accession number GU562296. Its unique sequence characteristics and its phylogenetic position suggest that PAstV-2 could be an important link between previously reported astroviruses and that a genetically divergent lineage of astroviruses exist in piglets.
Qingmei Xie - One of the best experts on this subject based on the ideXlab platform.
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characterization and genomic analysis of emerging astroviruses causing fatal gout in goslings
Transboundary and Emerging Diseases, 2020Co-Authors: Qinxi Chen, Chaoge Sui, Kejing Zuo, Guanglin Zhi, Lunguang Yao, Yunchao Kan, Qingmei XieAbstract:Since February 2017, severe outbreaks of fatal gout caused by novel gosling astroviruses (GoAstVs) have occurred in several Chinese provinces, causing a considerable economic impact on the poultry industry. To assess the infection status of GoAstVs causing gout, 165 clinical samples were collected from goslings from seven farms located in different Chinese provinces, and they were screened for viral infection. Seven GoAstV strains were completely sequenced. The positive infection rates of GoAstV, goose parvovirus, reovirus, goose haemorrhagic polyomavirus and Tembusu virus were 100%, 9.69%, 3.64%, 0% and 0%, respectively, indicating the role of GoAstV in gout. The genomes of all seven GoAstV strains were 7170-nt long and encoded three open reading frames (ORFs), namely, ORF1a, ORF1b and ORF2. Sequence and phylogenetic analyses of the seven GoAstV strains showed that these were Avastroviruses and were closely related to viruses classified within Avastrovirus 3 and turkey astrovirus 2. Moreover, the mutation rates of ORF1a and ORF2 were high, and ORF1a was highly mutated at amino acid loci 545-580. The tertiary structure of the mutated ORF2 protein was smooth, and its antigenic epitope was highly mutated, which may be related to the pathogenicity of the virus and caused by antibody pressure from the host. These findings enrich our understanding of the evolution of novel GoAstVs causing gout and their circulation as well as lay the foundation for the selection of vaccine strains.
Fumin Wang - One of the best experts on this subject based on the ideXlab platform.
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complete genome sequence of a novel Avastrovirus in goose
Archives of Virology, 2017Co-Authors: Yuxuan Zhang, Fumin Wang, Ning Liu, Lin Yang, Dabing ZhangAbstract:We report the complete genome sequence of a new Avastrovirus of goose-origin (FLX). The 7299-nt-long genome consisted of three overlapping open reading frames (ORFs) that were in different reading frames. Pairwise comparisons showed that the FLX genome was 59% identical to its closest relatives and that the levels of amino acid identity shared by FLX with other astroviruses did not exceed 54% in ORF1a, 66% in ORF1b, and 50% in ORF2, respectively. Phylogenetic analysis based on the amino acid sequence of the full-length ORF2 demonstrated that FLX was highly divergent from all other Avastroviruses. At the amino acid level the complete capsid region of FLX shared genetic distances of 0.574–0.719 with three official Avastrovirus species, suggesting that it can be classified as a member of a novel species in the genus Avastrovirus.
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genetic characterization of a novel astrovirus in pekin ducks
Infection Genetics and Evolution, 2015Co-Authors: Qinfeng Liao, Fumin Wang, Ning Liu, Xiaoyan Wang, Dabing ZhangAbstract:Three divergent groups of duck astroviruses (DAstVs), namely DAstV-1, DAstV-2 (formerly duck hepatitis virus type 3) and DAstV-3 (isolate CPH), and other Avastroviruses are known to infect domestic ducks. To provide more data regarding the molecular epidemiology of astroviruses in domestic ducks, we examined the prevalence of astroviruses in 136 domestic duck samples collected from four different provinces of China. Nineteen goose samples were also included. Using an astrovirus-specific reverse transcription-PCR assay, two groups of astroviruses were detected from our samples. A group of astroviruses detected from Pekin ducks, Shaoxing ducks and Landes geese were highly similar to the newly discovered DAstV-3. More interestingly, a novel group of Avastroviruses, which we named DAstV-4, was detected in Pekin ducks. Following full-length sequencing and sequence analysis, the variation between DAstV-4 and other Avastroviruses in terms of lengths of genome and internal component was highlighted. Sequence identity and phylogenetic analyses based on the amino acid sequences of the three open reading frames (ORFs) clearly demonstrated that DAstV-4 was highly divergent from all other Avastroviruses. Further analyses showed that DAstV-4 shared low levels of genome identities (50-58%) and high levels of mean amino acid genetic distances in the ORF2 sequences (0.520-0.801) with other Avastroviruses, suggesting DAstV-4 may represent an additional Avastrovirus species although the taxonomic relationship of DAstV-4 to DAstV-3 remains to be resolved. The present works contribute to the understanding of epidemiology, ecology and taxonomy of astroviruses in ducks.
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molecular characterization of a duck hepatitis virus 3 like astrovirus
Veterinary Microbiology, 2014Co-Authors: Ning Liu, Fumin Wang, Jiajian Shi, Lisha Zheng, Xiaoyan Wang, Dabing ZhangAbstract:Using an ORF1b-based astrovirus-specific reverse transcription (RT)-PCR assay, a duck hepatitis virus type 3 (DHV-3)-like astrovirus was detected from four intestinal samples collected from diseased ducks in China. Complete genome sequencing and comparative sequence analysis showed that the four duck astrovirus (DAstV) isolates were closely related and possessed a typical astrovirus genome organization. Genetic analysis of the complete ORF2 region revealed that mean amino acid genetic distances between the DHV-3-like isolates and previously known Avastrovirus species were between 0.579 and 0.721, suggesting that the DHV-3-like isolates could be classified as an additional Avastrovirus species. In the ORF1a and ORF1b regions, however, mean amino acid genetic distances between the DHV-3-like viruses and the turkey astrovirus 2 (TAstV-2)-like isolates were substantially less than those between TAstV-2-like isolates and DAstV/C-NGB-like astroviruses belonging to the same species. Pairwise comparisons and phylogenetic analyses demonstrated that the DHV-3-like isolates were most closely related to TAstV-2-like viruses in ORF1a and ORF1b, while showed highest similarity with the chicken astrovirus (CAstV) 612-like viruses in ORF2. These findings provide evidence that recombination events may have occurred during evolution of the Avastroviruses and support the view that genomic analysis is required for classification of the Avastroviruses.
