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Ernesto Fattorusso - One of the best experts on this subject based on the ideXlab platform.
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Further investigation of the Mediterranean sponge Axinella polypoides: isolation of a new cyclonucleoside and a new betaine.
Marine drugs, 2012Co-Authors: Marialuisa Menna, Ernesto Fattorusso, Anna Aiello, Paolo Luciano, Filomena D'aniello, Concetta Imperatore, Rocco VitaloneAbstract:An exhaustive exploration into the metabolic content of the Mediterranean sponge Axinella-polypoides resulted in the isolation of the new betaine 5 and the new cyclonucleoside 8. The structures of the new metabolites were elucidated by spectroscopic methods assisted by computational methods. The analysis also provided evidence that the sponge does not elaborate pyrrole-imidazole alkaloids (PIAs) but, interestingly, it was shown to contain two already known cyclodipeptides, compounds 9 (verpacamide A) and 10.
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and Rocco Vitalone
2012Co-Authors: Marialuisa Menna, Ernesto Fattorusso, Anna Aiello, Concetta Imperatore, Paolo LucianoAbstract:Abstract: An exhaustive exploration into the metabolic content of the Mediterranean sponge Axinella-polypoides resulted in the isolation of the new betaine 5 and the new cyclonucleoside 8. The structures of the new metabolites were elucidated by spectroscopic methods assisted by computational methods. The analysis also provided evidence that the sponge does not elaborate pyrrole-imidazole alkaloids (PIAs) but, interestingly, it was shown to contain two already known cyclodipeptides, compounds 9 (verpacamide A) and 10
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Polyaxibetaine, an amino acid derivative from the marine sponge Axinella polypoides.
Journal of natural products, 2010Co-Authors: Anna Aiello, Ernesto Fattorusso, Paolo Luciano, Marialuisa Menna, Rocco VitaloneAbstract:A new pyridinium derivative, polyaxibetaine (3), has been isolated from the marine sponge Axinella polypoides, together with two known modified amino acids, 1 and 2. The planar structure of compound 3 has been elucidated by spectroscopic methods; definition of the absolute configuration of compounds 1-3 has been carried out through ECD studies.
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Novel bioactive bromopyrrole alkaloids from the Mediterranean sponge Axinella verrucosa.
Bioorganic & medicinal chemistry, 2005Co-Authors: Anna Aiello, Ernesto Fattorusso, Marialuisa Menna, Monica D'esposito, Werner E. G. Müller, Sanja Perović-ottstadt, Heinz C. SchröderAbstract:The Mediterranean sponge Axinella verrucosa has been investigated for its alkaloid composition and has been found to produce a complex mixture of bromopyrrole alkaloids. Along with the previously isolated compounds 5-18, four novel alkaloids of this class, compounds 1-4, have been isolated, and their structures established through spectroscopic methods. Compounds 1-4 were found to display neuroprotective activity against the agonists serotonin and glutamate in vitro.
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Daminin, a bioactive pyrrole alkaloid from the Mediterranean sponge Axinella damicornis
Tetrahedron, 2005Co-Authors: Anna Aiello, Tobias A. M. Gulder, Ernesto Fattorusso, Marialuisa Menna, Monica D'esposito, Werner E. G. Müller, Sanja Perović-ottstadt, Hideyuki Tsuruta, Gerhard BringmannAbstract:Abstract The isolation and characterization of the known pyrrole alkaloid agelongine ( 6 ) and of the new natural product daminin ( 7 ), the bromine-free analogue of 6 , from a specimen of the marine sponge Axinella damicornis is described. Compound 7 showed significant neuroprotective properties. Moreover, for the supply of sufficient material for future medicinal investigations, a short total synthesis of 7 was developed.
Micha Ilan - One of the best experts on this subject based on the ideXlab platform.
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Additional file 2: of Mitochondrial group I and group II introns in the sponge orders Agelasida and Axinellida
2015Co-Authors: DorothĂŠe Huchon, Sigal Shefer, Micha Ilan, Amir Szitenberg, Tamar FeldsteinAbstract:Alignment of ORF 870. DNA sequence alignment, in Fasta format, of the ORF 870 of Tetilla radiata, Plakina trilopha, Plakina crypta, Agelas oroides and Axinella polypoides. (FASTA 5 kb
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Diversity and antibacterial activity of bacteria cultured from Mediterranean Axinella spp. sponges.
Journal of applied microbiology, 2013Co-Authors: Markus Haber, Micha IlanAbstract:Aims Evaluation of the diversity and antibacterial activity of bacteria cultivated from Mediterranean Axinella sponges and investigating the influence of culture conditions on antibacterial activity profiles of sponge bacteria. Methods and Results Based on 16S rRNA gene sequence analysis, the 259 bacteria isolated from the three Mediterranean Axinella sponges A. cannabina, A. verrucosa and A. polypoides belonged to 41 genera from the four phyla Actinobacteria, Bacteroidetes, Firmicutes and Proteobacteria and included five potential newly cultured genera. In antagonistic streak assays, 87 isolates (34%) from 13 genera showed antibacterial activity towards at least one of the 10 environmental and laboratory test bacteria. The extracts and filtrates of 22 isolates grown under three different culture conditions were less often active as the isolates in the corresponding antagonistic streak assays. Changes in antibacterial activity profiles were isolate- and culture condition-specific. Conclusions Axinella sponges are a good source to cultivate phylogenetic diverse and hitherto novel bacteria, many of which with antibacterial activity. Analysis of induced antibacterial activities might enhance the role of sponge bacteria in efforts to isolate new antibiotics in the future. Significance and Impact of the Study This study was the first to investigate the diversity and antibacterial activity of bacteria isolated from A. cannabina and A. verrucosa. It highlights the potential importance of induced activity and the need for employing multiple culture conditions in antibacterial screening assays of sponge-associated bacteria.
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Barnacle fouling in the Mediterranean sponges Axinella polypoides and Axinella verrucosa
Marine Ecology, 2013Co-Authors: Markus Haber, Amir Gur, Daniela Blihoghe, Micha IlanAbstract:Secondary metabolites protect many marine sponges (Phylum: Porifera) from settlement by fouling organisms. Previous studies on the subtidal demosponge Axinella verrucosa collected in the Western Mediterranean led to the isolation of compounds that inhibited the settlement of cyprids larvae of the intertidal barnacle Balanus amphitrite, and the enzyme chitinase, which plays a key role in the molting cycle of crustaceans. However, in a field survey conducted at three locations in Israel, Eastern Mediterranean Sea, we observed that A. verrucosa is fouled by the subtidal barnacle Balanus trigonus, a previously unknown association. Settlement inhibition assays using B. amphitrite with chemical extracts from Israeli A. verrucosa and Axinella polypoides, a sympatric, congeneric sponge that seems not to be fouled by B. trigonus, showed that cyprid larvae of B. amphitrite were inhibited by the extracts of both sponges from settlement at concentrations several magnitudes lower than natural volumetric extract concentration in the sponges. These results indicate that, unlike the intertidal barnacle B. amphitrite, the subtidal B. trigonus is unaffected by the compounds from A. verrucosa, stressing and underlining the importance of using suitable target organisms (i.e. from the same habitat) to test for ecologically relevant antifouling activities.
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Fulvitalea Axinellae gen. nov., sp. nov., a member of the family Flammeovirgaceae isolated from the Mediterranean sponge Axinella verrucosa.
International Journal of Systematic and Evolutionary Microbiology, 2012Co-Authors: Markus Haber, Sigal Shefer, Assunta Giordano, Pierangelo Orlando, Agata Gambacorta, Micha IlanAbstract:The yellow-pigmented, non-motile, Gram-negative, strictly aerobic, rod-shaped bacterial strain VI.18T was isolated from the Mediterranean sponge Axinella verrucosa collected off the coast near Sdot Yam, Israel. Results from 16S rRNA gene sequence analysis indicated that the isolate belonged to the family Flammeovirgaceae . The highest nucleotide similarity (91.4 %) occurred with Aureibacter tunicatorum A5Q-118T. The predominant cellular fatty acids of strain VI.18T were iso-C15 : 0 (56.0 %), iso-C17 : 1ω9c (22.8 %) and C16 : 0 (7.4 %) and its major respiratory quinone was MK-7. The DNA G+C content was 47.5 mol%. The strain could readily be distinguished from its phylogenetically closest relatives by phenotypic, physiological and chemotaxonomic properties. On the basis of the data from the present polyphasic study, we propose a novel genus and species within the family Flammeovirgaceae , with the name Fulvitalea Axinellae gen. nov., sp. nov. Strain VI.18T ( = ATCC BAA-2395T = LMG 26722T) is the type strain of Fulvitalea Axinellae.
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Structure of debromo-carteramine A, a novel bromopyrrole alkaloid from the Mediterranean sponge Axinella verrucosa
Arkivoc, 2010Co-Authors: Markus Haber, Micha Ilan, Marianna Carbone, Margherita GavagninAbstract:The butanol extract of the Mediterranean sponge Axinella verrucosa was fractionated via a bioactivity-guided procedure based on an antibacterial assay. This approach led to the isolation of the novel alkaloid debromo-carteramine A 2 co-occurring with known hymenidin 1, the main antibacterial agent of the extract, and related inactive carteramine A 3.
Anna Aiello - One of the best experts on this subject based on the ideXlab platform.
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Further investigation of the Mediterranean sponge Axinella polypoides: isolation of a new cyclonucleoside and a new betaine.
Marine drugs, 2012Co-Authors: Marialuisa Menna, Ernesto Fattorusso, Anna Aiello, Paolo Luciano, Filomena D'aniello, Concetta Imperatore, Rocco VitaloneAbstract:An exhaustive exploration into the metabolic content of the Mediterranean sponge Axinella-polypoides resulted in the isolation of the new betaine 5 and the new cyclonucleoside 8. The structures of the new metabolites were elucidated by spectroscopic methods assisted by computational methods. The analysis also provided evidence that the sponge does not elaborate pyrrole-imidazole alkaloids (PIAs) but, interestingly, it was shown to contain two already known cyclodipeptides, compounds 9 (verpacamide A) and 10.
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and Rocco Vitalone
2012Co-Authors: Marialuisa Menna, Ernesto Fattorusso, Anna Aiello, Concetta Imperatore, Paolo LucianoAbstract:Abstract: An exhaustive exploration into the metabolic content of the Mediterranean sponge Axinella-polypoides resulted in the isolation of the new betaine 5 and the new cyclonucleoside 8. The structures of the new metabolites were elucidated by spectroscopic methods assisted by computational methods. The analysis also provided evidence that the sponge does not elaborate pyrrole-imidazole alkaloids (PIAs) but, interestingly, it was shown to contain two already known cyclodipeptides, compounds 9 (verpacamide A) and 10
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Polyaxibetaine, an amino acid derivative from the marine sponge Axinella polypoides.
Journal of natural products, 2010Co-Authors: Anna Aiello, Ernesto Fattorusso, Paolo Luciano, Marialuisa Menna, Rocco VitaloneAbstract:A new pyridinium derivative, polyaxibetaine (3), has been isolated from the marine sponge Axinella polypoides, together with two known modified amino acids, 1 and 2. The planar structure of compound 3 has been elucidated by spectroscopic methods; definition of the absolute configuration of compounds 1-3 has been carried out through ECD studies.
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Novel bioactive bromopyrrole alkaloids from the Mediterranean sponge Axinella verrucosa.
Bioorganic & medicinal chemistry, 2005Co-Authors: Anna Aiello, Ernesto Fattorusso, Marialuisa Menna, Monica D'esposito, Werner E. G. Müller, Sanja Perović-ottstadt, Heinz C. SchröderAbstract:The Mediterranean sponge Axinella verrucosa has been investigated for its alkaloid composition and has been found to produce a complex mixture of bromopyrrole alkaloids. Along with the previously isolated compounds 5-18, four novel alkaloids of this class, compounds 1-4, have been isolated, and their structures established through spectroscopic methods. Compounds 1-4 were found to display neuroprotective activity against the agonists serotonin and glutamate in vitro.
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Daminin, a bioactive pyrrole alkaloid from the Mediterranean sponge Axinella damicornis
Tetrahedron, 2005Co-Authors: Anna Aiello, Tobias A. M. Gulder, Ernesto Fattorusso, Marialuisa Menna, Monica D'esposito, Werner E. G. Müller, Sanja Perović-ottstadt, Hideyuki Tsuruta, Gerhard BringmannAbstract:Abstract The isolation and characterization of the known pyrrole alkaloid agelongine ( 6 ) and of the new natural product daminin ( 7 ), the bromine-free analogue of 6 , from a specimen of the marine sponge Axinella damicornis is described. Compound 7 showed significant neuroprotective properties. Moreover, for the supply of sufficient material for future medicinal investigations, a short total synthesis of 7 was developed.
Marialuisa Menna - One of the best experts on this subject based on the ideXlab platform.
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Further investigation of the Mediterranean sponge Axinella polypoides: isolation of a new cyclonucleoside and a new betaine.
Marine drugs, 2012Co-Authors: Marialuisa Menna, Ernesto Fattorusso, Anna Aiello, Paolo Luciano, Filomena D'aniello, Concetta Imperatore, Rocco VitaloneAbstract:An exhaustive exploration into the metabolic content of the Mediterranean sponge Axinella-polypoides resulted in the isolation of the new betaine 5 and the new cyclonucleoside 8. The structures of the new metabolites were elucidated by spectroscopic methods assisted by computational methods. The analysis also provided evidence that the sponge does not elaborate pyrrole-imidazole alkaloids (PIAs) but, interestingly, it was shown to contain two already known cyclodipeptides, compounds 9 (verpacamide A) and 10.
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and Rocco Vitalone
2012Co-Authors: Marialuisa Menna, Ernesto Fattorusso, Anna Aiello, Concetta Imperatore, Paolo LucianoAbstract:Abstract: An exhaustive exploration into the metabolic content of the Mediterranean sponge Axinella-polypoides resulted in the isolation of the new betaine 5 and the new cyclonucleoside 8. The structures of the new metabolites were elucidated by spectroscopic methods assisted by computational methods. The analysis also provided evidence that the sponge does not elaborate pyrrole-imidazole alkaloids (PIAs) but, interestingly, it was shown to contain two already known cyclodipeptides, compounds 9 (verpacamide A) and 10
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Polyaxibetaine, an amino acid derivative from the marine sponge Axinella polypoides.
Journal of natural products, 2010Co-Authors: Anna Aiello, Ernesto Fattorusso, Paolo Luciano, Marialuisa Menna, Rocco VitaloneAbstract:A new pyridinium derivative, polyaxibetaine (3), has been isolated from the marine sponge Axinella polypoides, together with two known modified amino acids, 1 and 2. The planar structure of compound 3 has been elucidated by spectroscopic methods; definition of the absolute configuration of compounds 1-3 has been carried out through ECD studies.
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Novel bioactive bromopyrrole alkaloids from the Mediterranean sponge Axinella verrucosa.
Bioorganic & medicinal chemistry, 2005Co-Authors: Anna Aiello, Ernesto Fattorusso, Marialuisa Menna, Monica D'esposito, Werner E. G. Müller, Sanja Perović-ottstadt, Heinz C. SchröderAbstract:The Mediterranean sponge Axinella verrucosa has been investigated for its alkaloid composition and has been found to produce a complex mixture of bromopyrrole alkaloids. Along with the previously isolated compounds 5-18, four novel alkaloids of this class, compounds 1-4, have been isolated, and their structures established through spectroscopic methods. Compounds 1-4 were found to display neuroprotective activity against the agonists serotonin and glutamate in vitro.
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Daminin, a bioactive pyrrole alkaloid from the Mediterranean sponge Axinella damicornis
Tetrahedron, 2005Co-Authors: Anna Aiello, Tobias A. M. Gulder, Ernesto Fattorusso, Marialuisa Menna, Monica D'esposito, Werner E. G. Müller, Sanja Perović-ottstadt, Hideyuki Tsuruta, Gerhard BringmannAbstract:Abstract The isolation and characterization of the known pyrrole alkaloid agelongine ( 6 ) and of the new natural product daminin ( 7 ), the bromine-free analogue of 6 , from a specimen of the marine sponge Axinella damicornis is described. Compound 7 showed significant neuroprotective properties. Moreover, for the supply of sufficient material for future medicinal investigations, a short total synthesis of 7 was developed.
Markus Haber - One of the best experts on this subject based on the ideXlab platform.
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Diversity and antibacterial activity of bacteria cultured from Mediterranean Axinella spp. sponges.
Journal of applied microbiology, 2013Co-Authors: Markus Haber, Micha IlanAbstract:Aims Evaluation of the diversity and antibacterial activity of bacteria cultivated from Mediterranean Axinella sponges and investigating the influence of culture conditions on antibacterial activity profiles of sponge bacteria. Methods and Results Based on 16S rRNA gene sequence analysis, the 259 bacteria isolated from the three Mediterranean Axinella sponges A. cannabina, A. verrucosa and A. polypoides belonged to 41 genera from the four phyla Actinobacteria, Bacteroidetes, Firmicutes and Proteobacteria and included five potential newly cultured genera. In antagonistic streak assays, 87 isolates (34%) from 13 genera showed antibacterial activity towards at least one of the 10 environmental and laboratory test bacteria. The extracts and filtrates of 22 isolates grown under three different culture conditions were less often active as the isolates in the corresponding antagonistic streak assays. Changes in antibacterial activity profiles were isolate- and culture condition-specific. Conclusions Axinella sponges are a good source to cultivate phylogenetic diverse and hitherto novel bacteria, many of which with antibacterial activity. Analysis of induced antibacterial activities might enhance the role of sponge bacteria in efforts to isolate new antibiotics in the future. Significance and Impact of the Study This study was the first to investigate the diversity and antibacterial activity of bacteria isolated from A. cannabina and A. verrucosa. It highlights the potential importance of induced activity and the need for employing multiple culture conditions in antibacterial screening assays of sponge-associated bacteria.
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Barnacle fouling in the Mediterranean sponges Axinella polypoides and Axinella verrucosa
Marine Ecology, 2013Co-Authors: Markus Haber, Amir Gur, Daniela Blihoghe, Micha IlanAbstract:Secondary metabolites protect many marine sponges (Phylum: Porifera) from settlement by fouling organisms. Previous studies on the subtidal demosponge Axinella verrucosa collected in the Western Mediterranean led to the isolation of compounds that inhibited the settlement of cyprids larvae of the intertidal barnacle Balanus amphitrite, and the enzyme chitinase, which plays a key role in the molting cycle of crustaceans. However, in a field survey conducted at three locations in Israel, Eastern Mediterranean Sea, we observed that A. verrucosa is fouled by the subtidal barnacle Balanus trigonus, a previously unknown association. Settlement inhibition assays using B. amphitrite with chemical extracts from Israeli A. verrucosa and Axinella polypoides, a sympatric, congeneric sponge that seems not to be fouled by B. trigonus, showed that cyprid larvae of B. amphitrite were inhibited by the extracts of both sponges from settlement at concentrations several magnitudes lower than natural volumetric extract concentration in the sponges. These results indicate that, unlike the intertidal barnacle B. amphitrite, the subtidal B. trigonus is unaffected by the compounds from A. verrucosa, stressing and underlining the importance of using suitable target organisms (i.e. from the same habitat) to test for ecologically relevant antifouling activities.
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Fulvitalea Axinellae gen. nov., sp. nov., a member of the family Flammeovirgaceae isolated from the Mediterranean sponge Axinella verrucosa.
International Journal of Systematic and Evolutionary Microbiology, 2012Co-Authors: Markus Haber, Sigal Shefer, Assunta Giordano, Pierangelo Orlando, Agata Gambacorta, Micha IlanAbstract:The yellow-pigmented, non-motile, Gram-negative, strictly aerobic, rod-shaped bacterial strain VI.18T was isolated from the Mediterranean sponge Axinella verrucosa collected off the coast near Sdot Yam, Israel. Results from 16S rRNA gene sequence analysis indicated that the isolate belonged to the family Flammeovirgaceae . The highest nucleotide similarity (91.4 %) occurred with Aureibacter tunicatorum A5Q-118T. The predominant cellular fatty acids of strain VI.18T were iso-C15 : 0 (56.0 %), iso-C17 : 1ω9c (22.8 %) and C16 : 0 (7.4 %) and its major respiratory quinone was MK-7. The DNA G+C content was 47.5 mol%. The strain could readily be distinguished from its phylogenetically closest relatives by phenotypic, physiological and chemotaxonomic properties. On the basis of the data from the present polyphasic study, we propose a novel genus and species within the family Flammeovirgaceae , with the name Fulvitalea Axinellae gen. nov., sp. nov. Strain VI.18T ( = ATCC BAA-2395T = LMG 26722T) is the type strain of Fulvitalea Axinellae.
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Structure of debromo-carteramine A, a novel bromopyrrole alkaloid from the Mediterranean sponge Axinella verrucosa
Arkivoc, 2010Co-Authors: Markus Haber, Micha Ilan, Marianna Carbone, Margherita GavagninAbstract:The butanol extract of the Mediterranean sponge Axinella verrucosa was fractionated via a bioactivity-guided procedure based on an antibacterial assay. This approach led to the isolation of the novel alkaloid debromo-carteramine A 2 co-occurring with known hymenidin 1, the main antibacterial agent of the extract, and related inactive carteramine A 3.