The Experts below are selected from a list of 116433 Experts worldwide ranked by ideXlab platform
Takeshi Tokuhisa - One of the best experts on this subject based on the ideXlab platform.
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a role for Bcl6 in sequential class switch recomBination to ige in B Cells stimulated with il 4 and il 21
Molecular Immunology, 2008Co-Authors: Daisuke Kitayama, Akemi Sakamoto, Masafumi Arima, Masahiko Hatano, Masaru Miyazaki, Takeshi TokuhisaAbstract:ABstract IgE plays an important role in the pathogenesis of allergic diseases and high-affinity IgE memory B Cells are differentiated from IgG1 B Cells developed in germinal centers. Bcl6, a sequence specific transcriptional repressor, is highly expressed in germinal center B Cells and suppresses expression of Cɛ germline transcript. However, a role for Bcl6 in inhiBition of the sequential class switching from IgG1 to IgE in germinal center B Cells is not known. When splenic B Cells from Bcl6-deficient (Bcl6-KO) and Bcl6-transgenic (Bcl6-TG) mice were stimulated with anti-IgM AB and anti-CD40 AB plus IL-4, IgG1+IgE+ B Cells were detected in Bcl6-KO B Cell Culture But not in Bcl6-TG B Cell Culture. Cγ1 and Cɛ germline transcript in Bcl6-KO B Cells were induced earlier than those in wild-type (Bcl6-WT) B Cells after stimulation. When activated B Cells were simultaneously stimulated with IL-21, expression of Cγ1 germline transcript in Bcl6-WT and Bcl6-KO B Cells was enhanced By IL-21 stimulation, indicating that IL-21 is an enhancer of Cγ1 expression induced By IL-4. The amount of Cγ1 germline transcript in the Bcl6-KO B Cells was more than that in the Bcl6-WT B Cells. Conversely, IL-21 stimulation suppressed Cɛ expression in the Bcl6-WT B Cells. However, the suppression was not oBserved in the Bcl6-KO B Cells, suggesting that the IL-21-mediated suppression of Cɛ expression is due to Bcl6. Thus, Bcl6 controls the Cγ1 and Cɛ expression and staBilizes class switching to IgG1 in activated B Cells simultaneously stimulated with IL-4 and IL-21.
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A role for Bcl6 in sequential class switch recomBination to IgE in B Cells stimulated with IL-4 and IL-21.
Molecular immunology, 2007Co-Authors: Daisuke Kitayama, Akemi Sakamoto, Masafumi Arima, Masahiko Hatano, Masaru Miyazaki, Takeshi TokuhisaAbstract:IgE plays an important role in the pathogenesis of allergic diseases and high-affinity IgE memory B Cells are differentiated from IgG1 B Cells developed in germinal centers. Bcl6, a sequence specific transcriptional repressor, is highly expressed in germinal center B Cells and suppresses expression of Cvarepsilon germline transcript. However, a role for Bcl6 in inhiBition of the sequential class switching from IgG1 to IgE in germinal center B Cells is not known. When splenic B Cells from Bcl6-deficient (Bcl6-KO) and Bcl6-transgenic (Bcl6-TG) mice were stimulated with anti-IgM AB and anti-CD40 AB plus IL-4, IgG1(+)IgE(+) B Cells were detected in Bcl6-KO B Cell Culture But not in Bcl6-TG B Cell Culture. Cgamma1 and Cvarepsilon germline transcript in Bcl6-KO B Cells were induced earlier than those in wild-type (Bcl6-WT) B Cells after stimulation. When activated B Cells were simultaneously stimulated with IL-21, expression of Cgamma1 germline transcript in Bcl6-WT and Bcl6-KO B Cells was enhanced By IL-21 stimulation, indicating that IL-21 is an enhancer of Cgamma1 expression induced By IL-4. The amount of Cgamma1 germline transcript in the Bcl6-KO B Cells was more than that in the Bcl6-WT B Cells. Conversely, IL-21 stimulation suppressed Cvarepsilon expression in the Bcl6-WT B Cells. However, the suppression was not oBserved in the Bcl6-KO B Cells, suggesting that the IL-21-mediated suppression of Cvarepsilon expression is due to Bcl6. Thus, Bcl6 controls the Cgamma1 and Cvarepsilon expression and staBilizes class switching to IgG1 in activated B Cells simultaneously stimulated with IL-4 and IL-21.
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Role of I-A molecules in early stages of B Cell maturation.
Journal of immunology (Baltimore Md. : 1950), 1992Co-Authors: Nobuhiko Miki, Masahiko Hatano, K Wakita, S. Imoto, Shin-ichi Nishikawa, Takeshi TokuhisaAbstract:The role of the Ia molecule in the early phase of B Cell development remains controversial. In contrast to previous studies, we have detected minute amounts of Ia (I-A) molecule on early B lineage (B220+IgM-) Cells from normal Bone marrow, using ELISA. The presence of the I-A molecule even on pro-B Cells was deduced from experiments in which a monoclonal anti-I-A antiBody completely Blocked the generation of pre-B Cells from B progenitor (B220-) Cells in stromal Cell-dependent B Cell Culture. Inasmuch as this antiBody did not inhiBit the maturation of pre-B Cells to IgM+ B Cells in Culture, the I-A molecule on early B lineage Cells proBaBly plays a role in their maturation. We also examined the role of the I-A molecule in early B Cell development, using transgenic mice harBoring the antisense DNA to I-A Beta-chain gene. The amount of I-A molecule on splenic B Cells from the young transgenic mice decreased in the presence of aBundant amounts of the antisense RNA. B Cell development was perturBed in spleen from the transgenic mice. Stromal Cell-dependent B Cell Cultures from these mice clearly showed that the maturation of B lineage Cells was delayed at a very early stage of development (B220- to B220+). We propose that the I-A molecule on early B lineage Cells may play an essential role in their maturation.
Edouard Tuaillon - One of the best experts on this subject based on the ideXlab platform.
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comparison of eBv dna viral load in whole Blood plasma B Cells and B Cell Culture supernatant
Journal of Medical Virology, 2014Co-Authors: David Eric Ouedraogo, Karine Bollore, Johannes Viljoen, Vincent Foulongne, Jacques Reynes, Guillaume Cartron, Jean-pierre Vendrell, Philippe Van De Perre, Edouard TuaillonAbstract:Epstein–Barr virus (EBV) genome quantitation in whole Blood is used widely for therapeutic monitoring of EBV-associated disorders in immunosuppressed individuals and in patients with EBV-associated lymphoma. However, the most appropriate Biological material to Be used for EBV DNA quantitation remains a suBject of deBate. This study compare the detection rate and levels of EBV DNA from whole Blood, plasma, enriched B-Cells, and B-Cell short-term Culture supernatant using quantitative real-time PCR. Samples were collected from 33 suBjects with either HIV infection or B-Cell lymphoma. Overall, EBV DNA was detected in 100% of enriched B-Cell samples, in 82% of B-Cell Culture supernatants, in 57% of plasma, and 42% of whole Blood samples. A significant correlation for EBV viral load was found Between enriched B-Cell and B-Cell Culture supernatant material (ρ = 0.92; P < 0.0001), But no significant correlation existed Between EBV DNA levels in whole Blood and enriched B-Cells (ρ = −0.02; P = 0.89), whole Blood and plasma (ρ = 0.24; P = 0.24), or enriched B-Cells and plasma (ρ = 0.08; P = 0.77). Testing of enriched B-Cells appeared to Be the most sensitive method for detection of EBV DNA as well as for exploration of the Cellular reservoir. Quantitation of EBV DNA in plasma and B-Cell Culture supernatant may Be of interest to assess EBV reactivation dynamics and response to treatment as well as to decipher EBV host–pathogen interactions in various clinical scenarios. J. Med. Virol. 86:851–856, 2014. © 2013 Wiley Periodicals, Inc.
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Comparison of EBV DNA viral load in whole Blood, plasma, B-Cells and B-Cell Culture supernatant.
Journal of medical virology, 2013Co-Authors: David Eric Ouedraogo, Karine Bollore, Johannes Viljoen, Vincent Foulongne, Jacques Reynes, Guillaume Cartron, Jean-pierre Vendrell, Philippe Van De Perre, Edouard TuaillonAbstract:Epstein–Barr virus (EBV) genome quantitation in whole Blood is used widely for therapeutic monitoring of EBV-associated disorders in immunosuppressed individuals and in patients with EBV-associated lymphoma. However, the most appropriate Biological material to Be used for EBV DNA quantitation remains a suBject of deBate. This study compare the detection rate and levels of EBV DNA from whole Blood, plasma, enriched B-Cells, and B-Cell short-term Culture supernatant using quantitative real-time PCR. Samples were collected from 33 suBjects with either HIV infection or B-Cell lymphoma. Overall, EBV DNA was detected in 100% of enriched B-Cell samples, in 82% of B-Cell Culture supernatants, in 57% of plasma, and 42% of whole Blood samples. A significant correlation for EBV viral load was found Between enriched B-Cell and B-Cell Culture supernatant material (ρ = 0.92; P
Daisuke Kitayama - One of the best experts on this subject based on the ideXlab platform.
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a role for Bcl6 in sequential class switch recomBination to ige in B Cells stimulated with il 4 and il 21
Molecular Immunology, 2008Co-Authors: Daisuke Kitayama, Akemi Sakamoto, Masafumi Arima, Masahiko Hatano, Masaru Miyazaki, Takeshi TokuhisaAbstract:ABstract IgE plays an important role in the pathogenesis of allergic diseases and high-affinity IgE memory B Cells are differentiated from IgG1 B Cells developed in germinal centers. Bcl6, a sequence specific transcriptional repressor, is highly expressed in germinal center B Cells and suppresses expression of Cɛ germline transcript. However, a role for Bcl6 in inhiBition of the sequential class switching from IgG1 to IgE in germinal center B Cells is not known. When splenic B Cells from Bcl6-deficient (Bcl6-KO) and Bcl6-transgenic (Bcl6-TG) mice were stimulated with anti-IgM AB and anti-CD40 AB plus IL-4, IgG1+IgE+ B Cells were detected in Bcl6-KO B Cell Culture But not in Bcl6-TG B Cell Culture. Cγ1 and Cɛ germline transcript in Bcl6-KO B Cells were induced earlier than those in wild-type (Bcl6-WT) B Cells after stimulation. When activated B Cells were simultaneously stimulated with IL-21, expression of Cγ1 germline transcript in Bcl6-WT and Bcl6-KO B Cells was enhanced By IL-21 stimulation, indicating that IL-21 is an enhancer of Cγ1 expression induced By IL-4. The amount of Cγ1 germline transcript in the Bcl6-KO B Cells was more than that in the Bcl6-WT B Cells. Conversely, IL-21 stimulation suppressed Cɛ expression in the Bcl6-WT B Cells. However, the suppression was not oBserved in the Bcl6-KO B Cells, suggesting that the IL-21-mediated suppression of Cɛ expression is due to Bcl6. Thus, Bcl6 controls the Cγ1 and Cɛ expression and staBilizes class switching to IgG1 in activated B Cells simultaneously stimulated with IL-4 and IL-21.
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A role for Bcl6 in sequential class switch recomBination to IgE in B Cells stimulated with IL-4 and IL-21.
Molecular immunology, 2007Co-Authors: Daisuke Kitayama, Akemi Sakamoto, Masafumi Arima, Masahiko Hatano, Masaru Miyazaki, Takeshi TokuhisaAbstract:IgE plays an important role in the pathogenesis of allergic diseases and high-affinity IgE memory B Cells are differentiated from IgG1 B Cells developed in germinal centers. Bcl6, a sequence specific transcriptional repressor, is highly expressed in germinal center B Cells and suppresses expression of Cvarepsilon germline transcript. However, a role for Bcl6 in inhiBition of the sequential class switching from IgG1 to IgE in germinal center B Cells is not known. When splenic B Cells from Bcl6-deficient (Bcl6-KO) and Bcl6-transgenic (Bcl6-TG) mice were stimulated with anti-IgM AB and anti-CD40 AB plus IL-4, IgG1(+)IgE(+) B Cells were detected in Bcl6-KO B Cell Culture But not in Bcl6-TG B Cell Culture. Cgamma1 and Cvarepsilon germline transcript in Bcl6-KO B Cells were induced earlier than those in wild-type (Bcl6-WT) B Cells after stimulation. When activated B Cells were simultaneously stimulated with IL-21, expression of Cgamma1 germline transcript in Bcl6-WT and Bcl6-KO B Cells was enhanced By IL-21 stimulation, indicating that IL-21 is an enhancer of Cgamma1 expression induced By IL-4. The amount of Cgamma1 germline transcript in the Bcl6-KO B Cells was more than that in the Bcl6-WT B Cells. Conversely, IL-21 stimulation suppressed Cvarepsilon expression in the Bcl6-WT B Cells. However, the suppression was not oBserved in the Bcl6-KO B Cells, suggesting that the IL-21-mediated suppression of Cvarepsilon expression is due to Bcl6. Thus, Bcl6 controls the Cgamma1 and Cvarepsilon expression and staBilizes class switching to IgG1 in activated B Cells simultaneously stimulated with IL-4 and IL-21.
David Eric Ouedraogo - One of the best experts on this subject based on the ideXlab platform.
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comparison of eBv dna viral load in whole Blood plasma B Cells and B Cell Culture supernatant
Journal of Medical Virology, 2014Co-Authors: David Eric Ouedraogo, Karine Bollore, Johannes Viljoen, Vincent Foulongne, Jacques Reynes, Guillaume Cartron, Jean-pierre Vendrell, Philippe Van De Perre, Edouard TuaillonAbstract:Epstein–Barr virus (EBV) genome quantitation in whole Blood is used widely for therapeutic monitoring of EBV-associated disorders in immunosuppressed individuals and in patients with EBV-associated lymphoma. However, the most appropriate Biological material to Be used for EBV DNA quantitation remains a suBject of deBate. This study compare the detection rate and levels of EBV DNA from whole Blood, plasma, enriched B-Cells, and B-Cell short-term Culture supernatant using quantitative real-time PCR. Samples were collected from 33 suBjects with either HIV infection or B-Cell lymphoma. Overall, EBV DNA was detected in 100% of enriched B-Cell samples, in 82% of B-Cell Culture supernatants, in 57% of plasma, and 42% of whole Blood samples. A significant correlation for EBV viral load was found Between enriched B-Cell and B-Cell Culture supernatant material (ρ = 0.92; P < 0.0001), But no significant correlation existed Between EBV DNA levels in whole Blood and enriched B-Cells (ρ = −0.02; P = 0.89), whole Blood and plasma (ρ = 0.24; P = 0.24), or enriched B-Cells and plasma (ρ = 0.08; P = 0.77). Testing of enriched B-Cells appeared to Be the most sensitive method for detection of EBV DNA as well as for exploration of the Cellular reservoir. Quantitation of EBV DNA in plasma and B-Cell Culture supernatant may Be of interest to assess EBV reactivation dynamics and response to treatment as well as to decipher EBV host–pathogen interactions in various clinical scenarios. J. Med. Virol. 86:851–856, 2014. © 2013 Wiley Periodicals, Inc.
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Comparison of EBV DNA viral load in whole Blood, plasma, B-Cells and B-Cell Culture supernatant.
Journal of medical virology, 2013Co-Authors: David Eric Ouedraogo, Karine Bollore, Johannes Viljoen, Vincent Foulongne, Jacques Reynes, Guillaume Cartron, Jean-pierre Vendrell, Philippe Van De Perre, Edouard TuaillonAbstract:Epstein–Barr virus (EBV) genome quantitation in whole Blood is used widely for therapeutic monitoring of EBV-associated disorders in immunosuppressed individuals and in patients with EBV-associated lymphoma. However, the most appropriate Biological material to Be used for EBV DNA quantitation remains a suBject of deBate. This study compare the detection rate and levels of EBV DNA from whole Blood, plasma, enriched B-Cells, and B-Cell short-term Culture supernatant using quantitative real-time PCR. Samples were collected from 33 suBjects with either HIV infection or B-Cell lymphoma. Overall, EBV DNA was detected in 100% of enriched B-Cell samples, in 82% of B-Cell Culture supernatants, in 57% of plasma, and 42% of whole Blood samples. A significant correlation for EBV viral load was found Between enriched B-Cell and B-Cell Culture supernatant material (ρ = 0.92; P
Masahiko Hatano - One of the best experts on this subject based on the ideXlab platform.
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a role for Bcl6 in sequential class switch recomBination to ige in B Cells stimulated with il 4 and il 21
Molecular Immunology, 2008Co-Authors: Daisuke Kitayama, Akemi Sakamoto, Masafumi Arima, Masahiko Hatano, Masaru Miyazaki, Takeshi TokuhisaAbstract:ABstract IgE plays an important role in the pathogenesis of allergic diseases and high-affinity IgE memory B Cells are differentiated from IgG1 B Cells developed in germinal centers. Bcl6, a sequence specific transcriptional repressor, is highly expressed in germinal center B Cells and suppresses expression of Cɛ germline transcript. However, a role for Bcl6 in inhiBition of the sequential class switching from IgG1 to IgE in germinal center B Cells is not known. When splenic B Cells from Bcl6-deficient (Bcl6-KO) and Bcl6-transgenic (Bcl6-TG) mice were stimulated with anti-IgM AB and anti-CD40 AB plus IL-4, IgG1+IgE+ B Cells were detected in Bcl6-KO B Cell Culture But not in Bcl6-TG B Cell Culture. Cγ1 and Cɛ germline transcript in Bcl6-KO B Cells were induced earlier than those in wild-type (Bcl6-WT) B Cells after stimulation. When activated B Cells were simultaneously stimulated with IL-21, expression of Cγ1 germline transcript in Bcl6-WT and Bcl6-KO B Cells was enhanced By IL-21 stimulation, indicating that IL-21 is an enhancer of Cγ1 expression induced By IL-4. The amount of Cγ1 germline transcript in the Bcl6-KO B Cells was more than that in the Bcl6-WT B Cells. Conversely, IL-21 stimulation suppressed Cɛ expression in the Bcl6-WT B Cells. However, the suppression was not oBserved in the Bcl6-KO B Cells, suggesting that the IL-21-mediated suppression of Cɛ expression is due to Bcl6. Thus, Bcl6 controls the Cγ1 and Cɛ expression and staBilizes class switching to IgG1 in activated B Cells simultaneously stimulated with IL-4 and IL-21.
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A role for Bcl6 in sequential class switch recomBination to IgE in B Cells stimulated with IL-4 and IL-21.
Molecular immunology, 2007Co-Authors: Daisuke Kitayama, Akemi Sakamoto, Masafumi Arima, Masahiko Hatano, Masaru Miyazaki, Takeshi TokuhisaAbstract:IgE plays an important role in the pathogenesis of allergic diseases and high-affinity IgE memory B Cells are differentiated from IgG1 B Cells developed in germinal centers. Bcl6, a sequence specific transcriptional repressor, is highly expressed in germinal center B Cells and suppresses expression of Cvarepsilon germline transcript. However, a role for Bcl6 in inhiBition of the sequential class switching from IgG1 to IgE in germinal center B Cells is not known. When splenic B Cells from Bcl6-deficient (Bcl6-KO) and Bcl6-transgenic (Bcl6-TG) mice were stimulated with anti-IgM AB and anti-CD40 AB plus IL-4, IgG1(+)IgE(+) B Cells were detected in Bcl6-KO B Cell Culture But not in Bcl6-TG B Cell Culture. Cgamma1 and Cvarepsilon germline transcript in Bcl6-KO B Cells were induced earlier than those in wild-type (Bcl6-WT) B Cells after stimulation. When activated B Cells were simultaneously stimulated with IL-21, expression of Cgamma1 germline transcript in Bcl6-WT and Bcl6-KO B Cells was enhanced By IL-21 stimulation, indicating that IL-21 is an enhancer of Cgamma1 expression induced By IL-4. The amount of Cgamma1 germline transcript in the Bcl6-KO B Cells was more than that in the Bcl6-WT B Cells. Conversely, IL-21 stimulation suppressed Cvarepsilon expression in the Bcl6-WT B Cells. However, the suppression was not oBserved in the Bcl6-KO B Cells, suggesting that the IL-21-mediated suppression of Cvarepsilon expression is due to Bcl6. Thus, Bcl6 controls the Cgamma1 and Cvarepsilon expression and staBilizes class switching to IgG1 in activated B Cells simultaneously stimulated with IL-4 and IL-21.
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Role of I-A molecules in early stages of B Cell maturation.
Journal of immunology (Baltimore Md. : 1950), 1992Co-Authors: Nobuhiko Miki, Masahiko Hatano, K Wakita, S. Imoto, Shin-ichi Nishikawa, Takeshi TokuhisaAbstract:The role of the Ia molecule in the early phase of B Cell development remains controversial. In contrast to previous studies, we have detected minute amounts of Ia (I-A) molecule on early B lineage (B220+IgM-) Cells from normal Bone marrow, using ELISA. The presence of the I-A molecule even on pro-B Cells was deduced from experiments in which a monoclonal anti-I-A antiBody completely Blocked the generation of pre-B Cells from B progenitor (B220-) Cells in stromal Cell-dependent B Cell Culture. Inasmuch as this antiBody did not inhiBit the maturation of pre-B Cells to IgM+ B Cells in Culture, the I-A molecule on early B lineage Cells proBaBly plays a role in their maturation. We also examined the role of the I-A molecule in early B Cell development, using transgenic mice harBoring the antisense DNA to I-A Beta-chain gene. The amount of I-A molecule on splenic B Cells from the young transgenic mice decreased in the presence of aBundant amounts of the antisense RNA. B Cell development was perturBed in spleen from the transgenic mice. Stromal Cell-dependent B Cell Cultures from these mice clearly showed that the maturation of B lineage Cells was delayed at a very early stage of development (B220- to B220+). We propose that the I-A molecule on early B lineage Cells may play an essential role in their maturation.
