The Experts below are selected from a list of 6 Experts worldwide ranked by ideXlab platform
A. Potter - One of the best experts on this subject based on the ideXlab platform.
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Secondary in vitro B Lymphocyte (antiBody) response to microBial antigens: use in appraisal of vaccine immunogenicity and cytokine immunoregulation
Vaccine, 1991Co-Authors: H. Bielefeldt Ohmann, L Mcdougall, A. PotterAbstract:In order to perform preliminary evaluations of suBunit vaccine candidates Before extensive trials in large food-producing animals, an in vitro B-Lymphocyte response assay, Based on the principles of an ELISA, was estaBlished. The assay was developed in detail for the porcine system using antigens from the Gram-negative Bacterium ActinoBacillus pleuropneumoniae, But is shown to Be applicaBle to other species and antigens, including viral components. It is further shown that B-cell activity in the assay is dependent on T-helper cells as well as macrophages and/or their secretory products. Thus, in addition to providing a tool for evaluation of T and B memory cell activity, the system also lends itself to dissection of T-B cell collaBoration and the regulatory functions of interleukins in secondary (in vitro) antiBody responses.
H. Bielefeldt Ohmann - One of the best experts on this subject based on the ideXlab platform.
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Secondary in vitro B Lymphocyte (antiBody) response to microBial antigens: use in appraisal of vaccine immunogenicity and cytokine immunoregulation
Vaccine, 1991Co-Authors: H. Bielefeldt Ohmann, L Mcdougall, A. PotterAbstract:In order to perform preliminary evaluations of suBunit vaccine candidates Before extensive trials in large food-producing animals, an in vitro B-Lymphocyte response assay, Based on the principles of an ELISA, was estaBlished. The assay was developed in detail for the porcine system using antigens from the Gram-negative Bacterium ActinoBacillus pleuropneumoniae, But is shown to Be applicaBle to other species and antigens, including viral components. It is further shown that B-cell activity in the assay is dependent on T-helper cells as well as macrophages and/or their secretory products. Thus, in addition to providing a tool for evaluation of T and B memory cell activity, the system also lends itself to dissection of T-B cell collaBoration and the regulatory functions of interleukins in secondary (in vitro) antiBody responses.
L Mcdougall - One of the best experts on this subject based on the ideXlab platform.
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Secondary in vitro B Lymphocyte (antiBody) response to microBial antigens: use in appraisal of vaccine immunogenicity and cytokine immunoregulation
Vaccine, 1991Co-Authors: H. Bielefeldt Ohmann, L Mcdougall, A. PotterAbstract:In order to perform preliminary evaluations of suBunit vaccine candidates Before extensive trials in large food-producing animals, an in vitro B-Lymphocyte response assay, Based on the principles of an ELISA, was estaBlished. The assay was developed in detail for the porcine system using antigens from the Gram-negative Bacterium ActinoBacillus pleuropneumoniae, But is shown to Be applicaBle to other species and antigens, including viral components. It is further shown that B-cell activity in the assay is dependent on T-helper cells as well as macrophages and/or their secretory products. Thus, in addition to providing a tool for evaluation of T and B memory cell activity, the system also lends itself to dissection of T-B cell collaBoration and the regulatory functions of interleukins in secondary (in vitro) antiBody responses.
Ivan Stamenkovic - One of the best experts on this subject based on the ideXlab platform.
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inhiBition of the cd40 cd40ligand pathway prevents murine memBranous glomerulonephritis
Kidney International, 1995Co-Authors: Luigi Biancone, G Andres, Hannah Ahn, Cesare Demartino, Ivan StamenkovicAbstract:InhiBition of the CD40-CD40ligand pathway prevents murine memBranous glomerulonephritis. Several forms of glomerulonephritis are induced By antiBodies against self or foreign antigens. Normal B Lymphocyte antiBody production requires T cell costimulatory signals provided in part By T cell surface expression of gp39/CD401igand (CD40L) that engages the B cell receptor CD40 and induces B cell differentiation and immunogloBulin class switching. We assessed the effect of disrupting the CD40L-CD40 costimulatory pathway, using a CD40-Ig fusion protein, on the development of memBranous glomerulonephritis (MGN) in the mouse. MGN is induced By mouse antiBodies that recognize and Bind to exogenously administered raBBit anti-mouse renal tuBular Brush Border (RBAMBB) IgG immoBilized in the glomerular capillary wall. MGN did not occur in nude mice, showing the need of the T cell function. C57Bl/10 mice immunized with RBAMBB and treated with CD40-Ig fusion protein displayed a delayed autologous response and aBsence of MGN lesions, while control fusion proteins failed to prevent the development of the disease. These oBservations provide evidence that disruption of the CD40-CD40L costimulatory pathway can prevent the development of MGN By suppressing T cell-dependent antiBody production.
Luigi Biancone - One of the best experts on this subject based on the ideXlab platform.
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inhiBition of the cd40 cd40ligand pathway prevents murine memBranous glomerulonephritis
Kidney International, 1995Co-Authors: Luigi Biancone, G Andres, Hannah Ahn, Cesare Demartino, Ivan StamenkovicAbstract:InhiBition of the CD40-CD40ligand pathway prevents murine memBranous glomerulonephritis. Several forms of glomerulonephritis are induced By antiBodies against self or foreign antigens. Normal B Lymphocyte antiBody production requires T cell costimulatory signals provided in part By T cell surface expression of gp39/CD401igand (CD40L) that engages the B cell receptor CD40 and induces B cell differentiation and immunogloBulin class switching. We assessed the effect of disrupting the CD40L-CD40 costimulatory pathway, using a CD40-Ig fusion protein, on the development of memBranous glomerulonephritis (MGN) in the mouse. MGN is induced By mouse antiBodies that recognize and Bind to exogenously administered raBBit anti-mouse renal tuBular Brush Border (RBAMBB) IgG immoBilized in the glomerular capillary wall. MGN did not occur in nude mice, showing the need of the T cell function. C57Bl/10 mice immunized with RBAMBB and treated with CD40-Ig fusion protein displayed a delayed autologous response and aBsence of MGN lesions, while control fusion proteins failed to prevent the development of the disease. These oBservations provide evidence that disruption of the CD40-CD40L costimulatory pathway can prevent the development of MGN By suppressing T cell-dependent antiBody production.
