The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Dennis W. Wolan - One of the best experts on this subject based on the ideXlab platform.
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triflic acid treatment enables lc ms ms analysis of insoluble Bacterial Biomass
Journal of Proteome Research, 2018Co-Authors: Ana Y. Wang, Gregory S. Stupp, Peter S Thuyboun, Dennis W. WolanAbstract:The lysis and extraction of soluble Bacterial proteins from cells is a common practice for proteomics analyses, but insoluble Bacterial Biomasses are often left behind. Here, we show that with trif...
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Triflic acid treatment enables LC-MS/MS analysis of insoluble Bacterial Biomass
2018Co-Authors: Ana Y. Wang, Peter S. Thuy-boun, Gregory S. Stupp, Dennis W. WolanAbstract:The lysis and extraction of soluble Bacterial proteins from cells is a common practice for proteomics analyses, but insoluble Bacterial Biomasses are often left behind. Here, we show that with triflic acid treatment, the insoluble Bacterial Biomass of Gram- and Gram+ bacteria can be rendered soluble. We use LC-MS/MS shotgun proteomics to show that Bacterial proteins in the soluble and insoluble post-lysis fractions differ significantly. Additionally, in the case of Gram- Pseudomonas aeruginosa, triflic acid treatment enables the enrichment of cell envelope-associated proteins. Finally, we apply triflic acid to a human microbiome sample to show that this treatment is robust and enables the identification of a new, complementary subset of proteins from a complex microbial mixture.
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Triflic Acid Treatment Enables LC-MS/MS Analysis of Insoluble Bacterial Biomass
2018Co-Authors: Ana Y. Wang, Peter S. Thuy-boun, Gregory S. Stupp, Dennis W. WolanAbstract:The lysis and extraction of soluble Bacterial proteins from cells is a common practice for proteomics analyses, but insoluble Bacterial Biomasses are often left behind. Here, we show that with triflic acid treatment, the insoluble Bacterial Biomass of Gram– and Gram+ bacteria can be rendered soluble. We use LC-MS/MS shotgun proteomics to show that Bacterial proteins in the soluble and insoluble postlysis fractions differ significantly. Additionally, in the case of Gram– Pseudomonas aeruginosa, triflic acid treatment enables the enrichment of cell-envelope-associated proteins. Finally, we apply triflic acid to a human microbiome sample to show that this treatment is robust and enables the identification of a new, complementary subset of proteins from a complex microbial mixture
Pei Zhang - One of the best experts on this subject based on the ideXlab platform.
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invasive plants differentially affect soil biota through litter and rhizosphere pathways a meta analysis
Ecology Letters, 2019Co-Authors: Pei ZhangAbstract:Invasive plants affect soil biota through litter and rhizosphere inputs, but the direction and magnitude of these effects are variable. We conducted a meta-analysis to examine the different effects of litter and rhizosphere of invasive plants on soil communities and nutrient cycling. Our results showed that invasive plants increased Bacterial Biomass by 16%, detritivore abundance by 119% and microbivore abundance by 89% through litter pathway. In the rhizosphere, invasive plants reduced Bacterial Biomass by 12%, herbivore abundance by 55% and predator abundance by 52%, but increased AM fungal Biomass by 36%. Moreover, CO2 efflux, N mineralisation rate and enzyme activities were all higher in invasive than native rhizosphere soils. These findings indicate that invasive plants may support more decomposers that in turn stimulate nutrient release via litter effect, and enhance nutrient uptake by reducing root grazing but forming more symbioses in the rhizosphere. Thus, we hypothesise that litter- and root-based loops are probably linked to generate positive feedback of invaders on soil systems through stimulating nutrient cycling, consequently facilitating plant invasion. Our findings from limited cases with diverse contexts suggest that more studies are needed to differentiate litter and rhizosphere effects within single systems to better understand invasive plant-soil interactions.
Nabil Mameri - One of the best experts on this subject based on the ideXlab platform.
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denitrification of water in packed beds using Bacterial Biomass immobilized on waste plastics as supports
Ecological Engineering, 2013Co-Authors: A Cheikh, Nabil Mameri, A Yala, N Drouiche, N Abdi, H LouniciAbstract:a b s t r a c t The present work investigates a novel water denitrification technique based on immobilized Bacterial Biomass using various plastic wastes as supports PVC (polyvinyl chloride), HDPE (high density poly- ethylene) and LDPE (low density polyethylene) in packed columns. The efficiency of these supports is compared with a conventional support, granular activated carbon (GAC). The results obtained for denitri- fication under various operating conditions showed that working at a high fluid velocity does not affect the Bacterial behavior adversely. The denitrifying bacteria were able to treat water at an inlet nitrate concentration of 600 mg L −1 with removal efficiency near to 100%. The column packed with porous GAC as a support quickly became plugged in contrast to the nonporous LDPE which possesses a rough surface for film support and delivers almost the same performance as GAC. It was found that sulfate ions promote denitrification, but chloride inhibits it. The effectiveness of the packed-bed process is not diminished significantly by the coexistence of both types of ion in the feed stream.
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zinc uptake by streptomyces rimosus Biomass using a packed bed column
Journal of Chemical Technology & Biotechnology, 1999Co-Authors: L Addour, D. Belhocine, N Boudries, Yves Comeau, André Pauss, Nabil MameriAbstract:The ability of Streptomyces rimosus Biomass to bind zinc ions in batch mode was shown recently. The aim of this study was to determine the zinc uptake capacity by Streptomyces rimosus Biomass in continuous mode. Bacterial Biomass was able to bind more Zn(II) after pretreatment with sodium hydroxide (1 mol dm -3 ) than without treatment. The maximum adsorption capacity and the adsorption capacity at the saturation point calculated by means of both the exchange zone model and the Thomas model were practically identical of about 2.9 mg Zn(II) g -1 Biomass . This result was lower than the batch adsorption capacity of Streptomyces rimosus, indicating that the packed-bed is not the most appropriate process to exploit the Bacterial Biomass adsorption capacity. The effect of zinc concentration in the range of 10 to 200 mg Zn(II) dm -3 on the biosorption capacity of the packed-bed was not significant. Biomass regeneration with 0.1 mol dm -3 HCl gave a 90% recovery of the adsorbed Zn(II).
Ana Y. Wang - One of the best experts on this subject based on the ideXlab platform.
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triflic acid treatment enables lc ms ms analysis of insoluble Bacterial Biomass
Journal of Proteome Research, 2018Co-Authors: Ana Y. Wang, Gregory S. Stupp, Peter S Thuyboun, Dennis W. WolanAbstract:The lysis and extraction of soluble Bacterial proteins from cells is a common practice for proteomics analyses, but insoluble Bacterial Biomasses are often left behind. Here, we show that with trif...
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Triflic acid treatment enables LC-MS/MS analysis of insoluble Bacterial Biomass
2018Co-Authors: Ana Y. Wang, Peter S. Thuy-boun, Gregory S. Stupp, Dennis W. WolanAbstract:The lysis and extraction of soluble Bacterial proteins from cells is a common practice for proteomics analyses, but insoluble Bacterial Biomasses are often left behind. Here, we show that with triflic acid treatment, the insoluble Bacterial Biomass of Gram- and Gram+ bacteria can be rendered soluble. We use LC-MS/MS shotgun proteomics to show that Bacterial proteins in the soluble and insoluble post-lysis fractions differ significantly. Additionally, in the case of Gram- Pseudomonas aeruginosa, triflic acid treatment enables the enrichment of cell envelope-associated proteins. Finally, we apply triflic acid to a human microbiome sample to show that this treatment is robust and enables the identification of a new, complementary subset of proteins from a complex microbial mixture.
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Triflic Acid Treatment Enables LC-MS/MS Analysis of Insoluble Bacterial Biomass
2018Co-Authors: Ana Y. Wang, Peter S. Thuy-boun, Gregory S. Stupp, Dennis W. WolanAbstract:The lysis and extraction of soluble Bacterial proteins from cells is a common practice for proteomics analyses, but insoluble Bacterial Biomasses are often left behind. Here, we show that with triflic acid treatment, the insoluble Bacterial Biomass of Gram– and Gram+ bacteria can be rendered soluble. We use LC-MS/MS shotgun proteomics to show that Bacterial proteins in the soluble and insoluble postlysis fractions differ significantly. Additionally, in the case of Gram– Pseudomonas aeruginosa, triflic acid treatment enables the enrichment of cell-envelope-associated proteins. Finally, we apply triflic acid to a human microbiome sample to show that this treatment is robust and enables the identification of a new, complementary subset of proteins from a complex microbial mixture
Petr Baldrian - One of the best experts on this subject based on the ideXlab platform.
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cellulose utilization in forest litter and soil identification of Bacterial and fungal decomposers
FEMS Microbiology Ecology, 2012Co-Authors: Martina Stursova, Lucia žifcakova, Mary Beth Leigh, Robert Burgess, Petr BaldrianAbstract:Organic matter decomposition in the globally widespread coniferous forests has an important role in the carbon cycle, and cellulose decomposition is especially important in this respect because cellulose is the most abundant polysaccharide in plant litter. Cellulose decomposition was 10 times faster in the fungi-dominated litter of Picea abies forest than in the bacteria-dominated soil. In the soil, the added 13C-labelled cellulose was the main source of microbial respiration and was preferentially accumulated in the fungal Biomass and cellulose induced fungal proliferation. In contrast, in the litter, Bacterial Biomass showed higher labelling after 13C-cellulose addition and Bacterial Biomass increased. While 80% of the total community was represented by 104–106 Bacterial and 33–59 fungal operational taxonomic units (OTUs), 80% of the cellulolytic communities of bacteria and fungi were only composed of 8–18 highly abundant OTUs. Both the total and 13C-labelled communities differed substantially between the litter and soil. Cellulolytic bacteria in the acidic topsoil included Betaproteobacteria , Bacteroidetes and Acidobacteria , whereas these typically found in neutral soils were absent. Most fungal cellulose decomposers belonged to Ascomycota ; cellulolytic Basidiomycota were mainly represented by the yeasts Trichosporon and Cryptococcus . Several bacteria and fungi demonstrated here to derive their carbon from cellulose were previously not recognized as cellulolytic.
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saprotrophic basidiomycete mycelia and their interspecific interactions affect the spatial distribution of extracellular enzymes in soil
FEMS Microbiology Ecology, 2011Co-Authors: Jaroslav Šnajdr, Petra Dobiasova, Tomas Větrovský, Vendula Valaskova, Alaa Alawi, Lynne Boddy, Petr BaldrianAbstract:Saprotrophic cord-forming basidiomycetes are important decomposers of lignocellulosic substrates in soil. The production of extracellular hydrolytic enzymes was studied during the growth of two saprotrophic basidiomycetes, Hypholoma fasciculare and Phanerochaete velutina, across the surface of nonsterile soil microcosms, along with the effects of these basidiomycetes on fungi and bacteria within the soil. Higher activities of -glucosidase, β-glucosidase, cellobiohydrolase, β-xylosidase, phosphomonoesterase and phosphodiesterase, but not of arylsulphatase, were recorded beneath the mycelia. Despite the fact that H. fasciculare, with exploitative hyphal growth, produced much denser hyphal cover on the soil surface than P. velutina, with explorative growth, both fungi produced similar amounts of extracellular enzymes. In the areas where the mycelia of H. fasciculare and P. velutina interacted, the activities of N-acetylglucosaminidase, -glucosidase and phosphomonoesterase, the enzymes potentially involved in hyphal cell wall damage, and the utilization of compounds released from damaged hyphae of interacting fungi, were particularly increased. No significant differences in fungal Biomass were observed between basidiomycete-colonized and noncolonized soil, but Bacterial Biomass was reduced in soil with H. fasciculare. The increases in the activities of β-xylosidase, β-glucosidase, phosphomonoesterase and cellobiohydrolase with increasing fungal : Bacterial Biomass ratio indicate the positive effects of fungal enzymes on nutrient release and Bacterial abundance, which is reflected in the positive correlation of Bacterial and fungal Biomass content.
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Small-scale distribution of extracellular enzymes, fungal, and Bacterial Biomass in Quercus petraea forest topsoil
Biology and Fertility of Soils, 2010Co-Authors: Petr Baldrian, Věra Merhautová, Tomáš Cajthaml, Mirka Petránková, Jaroslav ŠnajdrAbstract:The small-scale distribution of activities of extracellular laccase, Mn-peroxidase, endoglucanase, cellobiohydrolase, β-glucosidase, endoxylanase, β-xylosidase, chitinase, and acid phosphatase were studied in the litter (L) and organic (H) horizons of Quercus petraea forest soil and related to the distribution of microbial Biomass. Geostatistical analysis showed that the spatial autocorrelation of the enzyme activities and soil microbial Biomass measured as phospholipid fatty acids (PLFA) and ergosterol content occurred at similar scales, typically in the range of tens of centimeters. The size of the spatial structures differed between the L and H horizons; for most of the studied enzymatic processes, litter exhibited a higher spatial variability (smaller autocorrelation distances). The distribution of several enzymes, including laccase, Mn-peroxidase, and some hydrolases, reflected the distribution of fungal Biomass. Polysaccharide hydrolases exhibited similar spatial distribution patterns in the L horizon, and their activity coincided with a high fungal/Bacterial Biomass ratio.