The Experts below are selected from a list of 540 Experts worldwide ranked by ideXlab platform
Al Kandari Sharifa - One of the best experts on this subject based on the ideXlab platform.
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Characterization and comparison of Campylobacter Bacteriophages
2013Co-Authors: Al Kandari SharifaAbstract:Members of the genus Campylobacter are a major cause of food-borne disease worldwide. They can colonize the intestinal mucosa of poultry, to high levels leading to contamination of meat, at slaughter. Their numbers can be reduced in different ways including chicken treatment with Bacteriophages. For such treatments to be successful, in depth understanding of the Bacteriophage that infects and kills campylobacters is vital. The work in this thesis describes: isolation and comprehensive characterisation of Bacteriophage candidates for future therapy applications. In order to increase the available stocks of characterized candidate Bacteriophage, a number of attempts were made to isolate Bacteriophages from poultry excreta. The new isolates together with some uncharacterized phages from our laboratory stocks were characterized with respect to their host range and genomic size. Some Bacteriophages preparations in previous studies showed genomes of different sizes and a number of attempts were done for their separation. This raised questions about the relationship between the two different sized genomes. Prior to this work, a co isolate pair had been successfully separated and the sequence of the larger genome, CP220, was determined. Part of the work here, was performed to extend this study by obta ining the sequence of the smaller co isolate, CPX and compare it to CP220. They did not appear to have any identifiable relationship at the genetic level, but the availability of the CPX sequence will further extend our knowledge of Bacteriophage Genetics and this phage has clear therapeutic potential. Attempts were also made to separate and characterize a second co-isolate pair but these were unsuccessful. The availability of the DNA sequence of CP220 allowed a much closer molecular characterisation and comparison of Campylobacter phage genomes, than had previously been possible. One area that was investigated in this study was the presence of repeat regions identified in the CP220 genome, which were amplified by PCR, but could not be cloned in E. coli. Furthermore, genes encoding potential lysins were identified in the CP220 genome and they were amplified, cloned and attempts were made to express the proteins, which may have potential therapeutic value. One gene product was successfully expressed and showed evidence of lytic activity on Campylobacter and other bacterial genera. In summary, this thesis describes a much closer examination of molecular biology of Campylobacter Bacteriophage than had previously been possible, including the determination of the sequence CPX phage.EThOS - Electronic Theses Online ServiceGBUnited Kingdo
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Characterization and comparison of Campylobacter Bacteriophages
2024Co-Authors: Al Kandari SharifaAbstract:Members of the genus Campylobacter are a major cause of food-borne disease worldwide. They can colonize the intestinal mucosa of poultry, to high levels leading to contamination of meat, at slaughter. Their numbers can be reduced in different ways including chicken treatment with Bacteriophages. For such treatments to be successful, in depth understanding of the Bacteriophage that infects and kills campylobacters is vital. The work in this thesis describes: isolation and comprehensive characterisation of Bacteriophage candidates for future therapy applications. In order to increase the available stocks of characterized candidate Bacteriophage, a number of attempts were made to isolate Bacteriophages from poultry excreta. The new isolates together with some uncharacterized phages from our laboratory stocks were characterized with respect to their host range and genomic size. Some Bacteriophages preparations in previous studies showed genomes of different sizes and a number of attempts were done for their separation. This raised questions about the relationship between the two different sized genomes. Prior to this work, a co isolate pair had been successfully separated and the sequence of the larger genome, CP220, was determined. Part of the work here, was performed to extend this study by obtaining the sequence of the smaller co isolate, CPX and compare it to CP220. They did not appear to have any identifiable relationship at the genetic level, but the availability of the CPX sequence will further extend our knowledge of Bacteriophage Genetics and this phage has clear therapeutic potential. Attempts were also made to separate and characterize a second co-isolate pair but these were unsuccessful. The availability of the DNA sequence of CP220 allowed a much closer molecular characterisation and comparison of Campylobacter phage genomes, than had previously been possible. One area that was investigated in this study was the presence of repeat regions identified in the CP220 genome, which were amplified by PCR, but could not be cloned in E. coli. Furthermore, genes encoding potential lysins were identified in the CP220 genome and they were amplified, cloned and attempts were made to express the proteins, which may have potential therapeutic value. One gene product was successfully expressed and showed evidence of lytic activity on Campylobacter and other bacterial genera. In summary, this thesis describes a much closer examination of molecular biology of Campylobacter Bacteriophage than had previously been possible, including the determination of the sequence CPX phage
Edward A Birge - One of the best experts on this subject based on the ideXlab platform.
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bacterial and Bacteriophage Genetics
1994Co-Authors: Edward A BirgeAbstract:Preface to the Fourth Edition * Fundamentals of Bacterial and Viral Genetics * Replication and Analysis of DNA * Mutations and Mutagenesis * Transcription and Translation: Processes and Basic Regulation * DNA Repair and Simple Recombination * T4 Bacteriophage as a Model Genetic System * Genetics of Other Intemperate Bacteriophages * Genetics of Temperate Bacteriophages * Transduction * Genetic Transformation * Conjugation and the Escherichia coli Paradigm * Other Plasmids and Other Conjugation Systems * Plasmid Molecular Biology * Advanced Regulatory Topics * Recombination: Generally and Specifically * Applied Bacterial Genetics * Bacterial Evolution * Glossary * Appendix * Index.
V N Krylov - One of the best experts on this subject based on the ideXlab platform.
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phagotherapy in terms of Bacteriophage Genetics hopes perspectives safety limitations
Russian Journal of Genetics, 2001Co-Authors: V N KrylovAbstract:The appearance and spreading of multidrug-resistant bacterial pathogens is a consequence of the large-scale use of antibiotics in medicine. In view of this, claims for the phage therapy were renewed: in recent studies, the natural phages and their products neutralizing various proteins, as well as the bacterial products often controlled by defective prophages (bacteriocins) were applied for treatment of bacterial infections. Constructs obtained by gene engineering are increasingly used to change Bacteriophage properties to expand the spectrum of their lytic activity and to eliminate therapeutic drawbacks of some natural phages. In this review, the problem of phage therapy is discussed in general with respect to Bacteriophage properties, their Genetics, structure, evolution, taking into account long-term experience of the author in the field of Bacteriophage Genetics. Note that the general concept of phage therapy should be developed to ensure long-term, efficient and harmless phage therapy.
Scot E Dowd - One of the best experts on this subject based on the ideXlab platform.
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Bacteriophage biology and Genetics
Encyclopedia of Environmental Microbiology, 2003Co-Authors: Scot E DowdAbstract:Terminology Taxonomy Structure and Pathogenesis Pathogenesis Basic Genetics of RNA and DNA Phage Keywords: Bacteriophage; Bacteriophage, Genetics of; Genetics of Bacteriophage; phage
Okekeogbu Ikenna - One of the best experts on this subject based on the ideXlab platform.
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Genome Characterization and Annotation of a Cluster S Bacteriophage
'Purdue University (bepress)', 2019Co-Authors: Georgiev Anna-nikol, Myers Kathryn, Jammer Kierra, Callin Morgan, Sorrel Taylor, Clase Kari, Okekeogbu IkennaAbstract:Bacteriophages (phages) are viruses that infect their host and cannot reproduce independently outside of them. The application of Bacteriophages in the biotechnology and medical sectors has recently increased, including uses as a potential antibacterial agent and CRISPR technology. In this project, the 48,667 to 58,636 base pair region (genes 87-98) of the Corazon phage genome was annotated by five student researchers at Purdue University. Corazon, a cluster S phage was isolated at Lafayette College in Easton, PA. Corazon belongs to the Siphoviridae morphotype and its genome consists of 109 genes. In this study, gene locations were called using evidence consisting of alignment results, coding potential, and comparison to other phage genomes using DNAMaster, NCBI Blast, Phamerator, HHPred, Starterator. The annotation of a genome consists of confidently assigning start sites and functions to genes based on evidence obtained from auto-annotation of the genome and additional evidence collected based on the genome cluster and similar calls in other phages. Notable functions include minor tail proteins, which are found in the tail fiber or sheath of the phage, and HNH endonuclease, which is a component of the phage packaging machinery. Many of the genes annotated have no known function based on the collected evidence but additional research may yield alternative results and additional uses. Further research of Bacteriophage Genetics allows deeper investigation and heightened understanding of their possible uses
