The Experts below are selected from a list of 15315 Experts worldwide ranked by ideXlab platform
Sophie Lauriau - One of the best experts on this subject based on the ideXlab platform.
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antimicrobial peptides released by enzymatic hydrolysis of hen egg white lysozyme
Journal of Agricultural and Food Chemistry, 2004Co-Authors: Yoshinori Mine, Sophie LauriauAbstract:This work was aimed at the isolation, purification, and characterization of novel antimicrobial peptides from chicken egg white lysozyme hydrolysate, obtained by peptic digestion and subsequent tryptic digestion. The hydrolysate was composed of over 20 small peptides of less than 1000 Da, and had no enzymatic activity. The water-soluble peptide mixture showed Bacteriostatic activity against Gram-positive bacteria (Staphylococcus aureus 23-394) and Gram-negative bacteria (Escherichia coli K-12). Two Bacteriostatic peptides were purified and sequenced. One peptide, with the sequence Ile-Val-Ser-Asp-Gly-Asp-Gly-Met-Asn-Ala-Trp, inhibited Gram-negative bacteria E. coli K-12 and corresponded to amino acid residues 98-108, which are located in the middle part of the helix-loop-helix. Another novel antimicrobial peptide inhibited S. aureus 23-394 and was determined to have the sequence His-Gly-Leu-Asp-Asn-Tyr-Arg, corresponding to amino acid residues 15-21 of lysozyme. These peptides broadened the antimicrobial activity of lysozyme to include Gram-negative bacteria. The results obtained in this study indicate that lysozyme possesses nonenzymatic Bacteriostatic domains in its primary sequence and they are released by proteolytic hydrolysis.
Veronika Hola - One of the best experts on this subject based on the ideXlab platform.
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following the mechanisms of Bacteriostatic versus bactericidal action using raman spectroscopy
Molecules, 2013Co-Authors: Silvie Bernatova, Ota Samek, Zdeněk Pilat, Mojmir Serý, Jan Ježek, P Jakl, Martin Siler, Vladislav Krzyžanek, Pavel Zemanek, Veronika HolaAbstract:Antibiotics cure infections by influencing bacterial growth or viability. Antibiotics can be divided to two groups on the basis of their effect on microbial cells through two main mechanisms, which are either bactericidal or Bacteriostatic. Bactericidal antibiotics kill the bacteria and Bacteriostatic antibiotics suppress the growth of bacteria (keep them in the stationary phase of growth). One of many factors to predict a favorable clinical outcome of the potential action of antimicrobial chemicals may be provided using in vitro bactericidal/Bacteriostatic data (e.g., minimum inhibitory concentrations-MICs). Consequently, MICs are used in clinical situations mainly to confirm resistance, and to determine the in vitro activities of new antimicrobials. We report on the combination of data obtained from MICs with information on microorganisms' "fingerprint" (e.g., DNA/RNA, and proteins) provided by Raman spectroscopy. Thus, we could follow mechanisms of the Bacteriostatic versus bactericidal action simply by detecting the Raman bands corresponding to DNA. The Raman spectra of Staphylococcus epidermidis treated with clindamycin (a Bacteriostatic agent) indeed show little effect on DNA which is in contrast with the action of ciprofloxacin (a bactericidal agent), where the Raman spectra show a decrease in strength of the signal assigned to DNA, suggesting DNA fragmentation.
Yoshinori Mine - One of the best experts on this subject based on the ideXlab platform.
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antimicrobial peptides released by enzymatic hydrolysis of hen egg white lysozyme
Journal of Agricultural and Food Chemistry, 2004Co-Authors: Yoshinori Mine, Sophie LauriauAbstract:This work was aimed at the isolation, purification, and characterization of novel antimicrobial peptides from chicken egg white lysozyme hydrolysate, obtained by peptic digestion and subsequent tryptic digestion. The hydrolysate was composed of over 20 small peptides of less than 1000 Da, and had no enzymatic activity. The water-soluble peptide mixture showed Bacteriostatic activity against Gram-positive bacteria (Staphylococcus aureus 23-394) and Gram-negative bacteria (Escherichia coli K-12). Two Bacteriostatic peptides were purified and sequenced. One peptide, with the sequence Ile-Val-Ser-Asp-Gly-Asp-Gly-Met-Asn-Ala-Trp, inhibited Gram-negative bacteria E. coli K-12 and corresponded to amino acid residues 98-108, which are located in the middle part of the helix-loop-helix. Another novel antimicrobial peptide inhibited S. aureus 23-394 and was determined to have the sequence His-Gly-Leu-Asp-Asn-Tyr-Arg, corresponding to amino acid residues 15-21 of lysozyme. These peptides broadened the antimicrobial activity of lysozyme to include Gram-negative bacteria. The results obtained in this study indicate that lysozyme possesses nonenzymatic Bacteriostatic domains in its primary sequence and they are released by proteolytic hydrolysis.
Bingshan Zeng - One of the best experts on this subject based on the ideXlab platform.
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Chlorothalonil: an effective Bacteriostatic agent for bud induction of Acacia auriculiformis under open condition (non-axenic)
Plant Methods, 2019Co-Authors: Liejian Huang, Muhammad Qasim Shahid, Hong Wang, Bingshan ZengAbstract:BackgroundOpen tissue culture technique could be simplified by using different Bacteriostatic agents. There is a great difference in the Bacteriostatic effects of different antimicrobial agents on various explants. However, there is no report about the effective Bacteriostatic agent for open tissue culture of Acacia auriculiformis.ResultsWe carried out the bud induction trials under open conditions to screen out an effective antibacterial agent for open tissue culture of A. auriculiformis. The results showed that the suitable type and concentration of Bacteriostatic agent was 0.2 g L−1 Chlorothalonil, and the suitable explant type was middle shoot section with leaves (the shoot section with third to fifth axillary bud). The treatment of 0.8 g L−1 Carbendazim for 3 min was the most suitable strategy for explants disinfection, and October was the best time for explants collection. The suitable bud induction medium was 1/8 MS + agar 7 g L−1 + Chlorothalonil 0.2 g L−1 + 6-BA 1.5 mg L−1, and the bud induction rate was 99.54%.ConclusionsOur results revealed that Chlorothalonil is an effective Bacteriostatic agent for bud induction of A. auriculiformis under open condition. These results would be very helpful for further establishment of open tissue culture technology for A. auriculiformis.
Renata Cegielskaradziejewska - One of the best experts on this subject based on the ideXlab platform.
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ultrafiltration modified chicken egg white lysozyme and its antibacterial action
International Journal of Food Science and Technology, 2009Co-Authors: Grzegorz Lesnierowski, Jacek Kijowski, Renata CegielskaradziejewskaAbstract:Summary The aim of the study was to produce a lysozyme preparation with an increased content of enzyme polymeric forms using the ultrafiltration (UF) technique. The effect of selected parameters of the membrane process on the extent of enzyme modification was examined. The study showed that the membrane technique, developed for the production of lysozyme monomer, could also be used in the direct production of a preparation containing enzyme polymers. Under optimal conditions of the modification procedure, the obtained preparation contained 53.3% of lysozyme polymeric forms. The effectiveness of the antibacterial action of UF-modified lysozyme against selected strains of bacteria was determined. Its Bacteriostatic activity depended on the applied modification conditions. Among lysozyme preparations modified by UF, the highest Bacteriostatic activity against selected strains of bacteria was recorded in the preparation containing 53.3% polymeric forms. The modification procedure facilitates the extension of the antibacterial spectrum of lysozyme against Gram-negative bacteria (Pseudomonas fluorescens).
