Bacterium Isolate

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M J Lami - One of the best experts on this subject based on the ideXlab platform.

  • pseudomonas stutzeri mjl19 a rhizosphere colonizing Bacterium that promotes plant growth under saline stress
    Journal of Applied Microbiology, 2020
    Co-Authors: M J Lami, C Adler, M Caramdi C Santo, A M Zenoff, R E De Cristobal, Manuel Espinosaurgel, P A Vincent
    Abstract:

    Aims The aim of this study was to find and use rhizobacteria able to confer plants advantages to deal with saline conditions. Methods and results We Isolated 24 different bacterial species from the rhizosphere of halophyte plants growing in Santiago del Estero, Argentina salt flat. Four strains were selected upon their ability to grow in salinity and their biochemical traits associated with plant growth promotion. Next, we tested the adhesion on soybean seeds surface and root colonization with the four selected Isolates. Isolate 19 stood out from the rest and was selected for further experiments. This strain showed positive chemotaxis towards soybean root exudates and a remarkable ability to form biofilm both in vitro conditions and on soybean roots. Interestingly, this trait was enhanced in high saline conditions, indicating the extremely adapted nature of the Bacterium to high salinity. In addition, this strain positively impacted on seed germination, plant growth and general plant health status also under saline stress. Conclusions A Bacterium Isolate with outstanding ability to promote seed germination and plant growth under saline conditions was found. Significance and impact of the study The experimental approach allowed us to find a suitable bacterial candidate for a biofertilizer intended to alleviate saline stress on crops. This would allow the use of soil now considered inadequate for agriculture and thus prevent further advancement of agriculture frontiers into areas of environmental value.

Seong-yun Jeong - One of the best experts on this subject based on the ideXlab platform.

  • Isolation of an algicide from a marine Bacterium and its effects against the toxic dinoflagellate Alexandrium catenella and other harmful algal bloom species
    Journal of Microbiology, 2015
    Co-Authors: Seong-yun Jeong
    Abstract:

    The aim of this study was to Isolate and identify bacteria demonstrating an algicidal effect against Alexandrium catenella and to determine the activity and range of any algicide discovered. The morphological and biochemical attributes of an algicidal Bacterium, Isolate YS-3, and analysis of its 16S rRNA gene sequence revealed it to be a member of the genus BrachyBacterium . This organism, designated BrachyBacterium sp. YS-3, showed the greatest effect against A. catenella cells of all bacteria Isolated, and is assumed to produce secondary metabolites. When 10% solutions of culture filtrates from this strain were applied to A. catenella cultures, over 90% of cells were killed within 9 h. Bioassay-guided isolation of the algicide involved led to the purification and identification of an active compound. Based on physicochemical and spectroscopic data, including nuclear magnetic resonance and mass analyses, this compound was identified as 1-acetyl-β-carboline. This algicide showed significant activity against A. catenella and a wide range of harmful algal bloom (HAB)-forming species. Taken together, our results suggest that BrachyBacterium sp. YS-3 and its algicide represent promising candidates for use in HAB control.

P A Vincent - One of the best experts on this subject based on the ideXlab platform.

  • pseudomonas stutzeri mjl19 a rhizosphere colonizing Bacterium that promotes plant growth under saline stress
    Journal of Applied Microbiology, 2020
    Co-Authors: M J Lami, C Adler, M Caramdi C Santo, A M Zenoff, R E De Cristobal, Manuel Espinosaurgel, P A Vincent
    Abstract:

    Aims The aim of this study was to find and use rhizobacteria able to confer plants advantages to deal with saline conditions. Methods and results We Isolated 24 different bacterial species from the rhizosphere of halophyte plants growing in Santiago del Estero, Argentina salt flat. Four strains were selected upon their ability to grow in salinity and their biochemical traits associated with plant growth promotion. Next, we tested the adhesion on soybean seeds surface and root colonization with the four selected Isolates. Isolate 19 stood out from the rest and was selected for further experiments. This strain showed positive chemotaxis towards soybean root exudates and a remarkable ability to form biofilm both in vitro conditions and on soybean roots. Interestingly, this trait was enhanced in high saline conditions, indicating the extremely adapted nature of the Bacterium to high salinity. In addition, this strain positively impacted on seed germination, plant growth and general plant health status also under saline stress. Conclusions A Bacterium Isolate with outstanding ability to promote seed germination and plant growth under saline conditions was found. Significance and impact of the study The experimental approach allowed us to find a suitable bacterial candidate for a biofertilizer intended to alleviate saline stress on crops. This would allow the use of soil now considered inadequate for agriculture and thus prevent further advancement of agriculture frontiers into areas of environmental value.

Tao Yang - One of the best experts on this subject based on the ideXlab platform.

  • a novel pb resistant bacillus subtilis Bacterium Isolate for co biosorption of hazardous sb iii and pb ii thermodynamics and application strategy
    International Journal of Environmental Research and Public Health, 2018
    Co-Authors: Yue Cai, Dongying Liu, Xuemeng Sun, Meng Zhang, Yu Gao, Yuchao Zhang, Tao Yang, Jingzhi Wang, Lijun Wang
    Abstract:

    The present work is the first to study co-biosorption of Pb(II) and Sb(III) by a novel Bacterium and its application strategy. The biosorption characteristics of Pb(II) and Sb(III) ions from aqueous solution using B. subtilis were investigated. Optimum pH, biomass dosage, contact time and temperature were determined to be 5.00, 6.00 mg/L, 45 min and 35 °C, respectively. Langmuir, Freundlich, Temkin and Dubinin-Radushkevich (D-R) models were applied to describe the biosorption isotherm of the metal ions by B. subtilis. Results showed that Langmuir model fitted the equilibrium data of Pb(II) better than others, while biosorption of Sb(III) obeyed the Freundlich model well. The biosorption capacity of B. subtilis biomass for Pb(II) and Sb(III) ions was found to be 17.34 ± 0.14 and 2.32 ± 0.30 mg/g, respectively. Kinetic data showed the biosorption process of Pb(II) and Sb(III) ions both followed the pseudo-second-order kinetic model, with R2 ranging from 0.974 to 0.999 for Pb(II) and from 0.967 to 0.979 for Sb(III). The calculated thermodynamic parameters, negative ∆G and positive ∆H and ∆S values, indicated the biosorption of Pb(II) and Sb(III) ions onto B. subtilis biomass in water was feasible, endothermic, and spontaneous. Bacterial bioleaching experiment revealed B. subtilis can increase the mobility of Pb(II) and Sb(III) in polluted soil when pH was close to 6 at low temperature. Consequently, B. subtilis, as a cheap and original bacterial material, could be a promising biomass to remove Pb or Isolate Sb from industrial wastewater and to assist phytoremediation of Pb and Sb from weak acid or near neutral pH polluted soils at low temperature.

  • a novel high biosorbent of pb resistant Bacterium Isolate for the removal of hazardous lead from alkaline soil and water biosorption isotherms in vivo and bioremediation strategy
    Geomicrobiology Journal, 2018
    Co-Authors: Xianyu Liu, Dongying Liu, Hongxiang Bao, Yanan Zhao, Tao Yang
    Abstract:

    A novel Pb-resistant Bacterium was Isolated from aged lead-contaminated alkaline soils, and was identified as Bacillus megaterium via the MIDI protocol. The biosorption isotherms and kinetics of Pb...

Lijun Wang - One of the best experts on this subject based on the ideXlab platform.

  • a novel pb resistant bacillus subtilis Bacterium Isolate for co biosorption of hazardous sb iii and pb ii thermodynamics and application strategy
    International Journal of Environmental Research and Public Health, 2018
    Co-Authors: Yue Cai, Dongying Liu, Xuemeng Sun, Meng Zhang, Yu Gao, Yuchao Zhang, Tao Yang, Jingzhi Wang, Lijun Wang
    Abstract:

    The present work is the first to study co-biosorption of Pb(II) and Sb(III) by a novel Bacterium and its application strategy. The biosorption characteristics of Pb(II) and Sb(III) ions from aqueous solution using B. subtilis were investigated. Optimum pH, biomass dosage, contact time and temperature were determined to be 5.00, 6.00 mg/L, 45 min and 35 °C, respectively. Langmuir, Freundlich, Temkin and Dubinin-Radushkevich (D-R) models were applied to describe the biosorption isotherm of the metal ions by B. subtilis. Results showed that Langmuir model fitted the equilibrium data of Pb(II) better than others, while biosorption of Sb(III) obeyed the Freundlich model well. The biosorption capacity of B. subtilis biomass for Pb(II) and Sb(III) ions was found to be 17.34 ± 0.14 and 2.32 ± 0.30 mg/g, respectively. Kinetic data showed the biosorption process of Pb(II) and Sb(III) ions both followed the pseudo-second-order kinetic model, with R2 ranging from 0.974 to 0.999 for Pb(II) and from 0.967 to 0.979 for Sb(III). The calculated thermodynamic parameters, negative ∆G and positive ∆H and ∆S values, indicated the biosorption of Pb(II) and Sb(III) ions onto B. subtilis biomass in water was feasible, endothermic, and spontaneous. Bacterial bioleaching experiment revealed B. subtilis can increase the mobility of Pb(II) and Sb(III) in polluted soil when pH was close to 6 at low temperature. Consequently, B. subtilis, as a cheap and original bacterial material, could be a promising biomass to remove Pb or Isolate Sb from industrial wastewater and to assist phytoremediation of Pb and Sb from weak acid or near neutral pH polluted soils at low temperature.