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Matthew D. Collins - One of the best experts on this subject based on the ideXlab platform.
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Reclassification of EuBacterium formicigenerans Holdeman and Moore 1974 as Dorea formicigenerans gen. nov., comb. nov., and description of Dorea longicatena sp. nov., isolated from human faeces.
International Journal of Systematic and Evolutionary Microbiology, 2002Co-Authors: David Taras, Rainer Simmering, Matthew D. Collins, Paul A. Lawson, Michael BlautAbstract:Two strains of a gram-positively staining, obligately anaerobic, non-spore-forming, rod-shaped Bacterium, designated strains 111-13A and 111-35T, were isolated from human faeces. Analysis of the 16S rRNA gene sequences indicated that these strains were members of the Clostridium coccoides rRNA group of organisms. The nearest relatives of the unknown Bacterium were EuBacterium formicigenerans (having a sequence similarity of 94%) and an uncultured Bacterium (similarity > 99%). Characterization studies indicated that the unidentified faecal Bacterium was biochemically distinct from EuBacterium formicigenerans, members of the Clostridium coccoides group and all other described EuBacterium species. On the basis of the data from these studies, it is proposed that the hitherto unknown rod-shaped Bacterium be designated a species of a novel genus, namely Dorea longicatena gen. nov., sp. nov., and that EuBacterium formicigenerans be transferred to this genus as Dorea formicigenerans gen. nov., comb. nov.
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Arthrobacter rhombi sp. nov., isolated from Greenland halibut (Reinhardtius hippoglossoides).
International Journal of Systematic and Evolutionary Microbiology, 1999Co-Authors: Carlos R. Osorio, Juan L. Barja, Roger A. Hutson, Matthew D. CollinsAbstract:Two strains of a hitherto undescribed Gram-positive coryneform Bacterium isolated from Greenland halibut (Reinhardtius hippoglossoides) were characterized by phenotypic and molecular taxonomic methods. Comparative 16S rRNA gene sequencing studies demonstrated that the unknown strains constitute a new line within the genus Arthrobacter. The nearest relatives of the Bacterium from fish were members of the Arthrobacter nicotianael Arthrobacter sulfureus group. The unknown Bacterium was readily distinguished from these species by phenotypic methods. Based on phylogenetic and phenotypic evidence, it is proposed that the unknown Bacterium be classified as Arthrobacter rhombi sp. nov. The type strain of Arthrobacter rhombi is CCUG 38813T.
M D Collins - One of the best experts on this subject based on the ideXlab platform.
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phocoenobacter uteri gen nov sp nov a new member of the family pasteurellaceae pohl 1979 1981 isolated from a harbour porpoise phocoena phocoena
International Journal of Systematic and Evolutionary Microbiology, 2000Co-Authors: Geoffrey Foster, Roger A. Hutson, H M Ross, Henry Malnick, Anne Willems, R J Reid, M D CollinsAbstract:Phenotypic and phylogenetic studies were performed on a Gram-negative, rod-shaped Bacterium isolated from the uterus of a porpoise. Biochemical and physiological studies indicated that the Bacterium was related to the family Pasteurellaceae. Comparative 16S rRNA gene sequencing studies confirmed these findings and demonstrated that the Bacterium represents a hitherto unknown subline within this family of organisms. Based on the results of the phylogenetic analysis and phenotypic criteria, it is proposed that the Bacterium be assigned to a new genus, Phocoenobacter uteri gen. nov., sp. nov. The type strain of Phocoenobacter uteri sp. nov. is NCTC 12872T.
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actinobacillus scotiae sp nov a new member of the family pasteurellaceae pohl 1979 1981 isolated from porpoises phocoena phocoena
International Journal of Systematic and Evolutionary Microbiology, 1998Co-Authors: Geoffrey Foster, Roger A. Hutson, H M Ross, I A P Patterson, M D CollinsAbstract:Phenotypic and phylogenetic studies were performed on a Gram-negative, rod-shaped Bacterium isolated from three porpoises. Biochemical and physiological studies indicated that the Bacterium was related to the family Pasteurellaceae. Comparative 16S rRNA gene sequencing studies confirmed these findings and demonstrated that the Bacterium represents a hitherto unknown subline. The nearest phylogenetic relative of the unknown Bacterium wasActinobacillus delphinicola, an organism also originating from sea mammals, although a sequence divergence of 3% demonstrated that the newly isolated Bacterium is a distinct species. On the basis of the results of the phylogenetic analysis and phenotypic criteria, it is proposed that the Bacterium should be classified as a new species, Actinobacillus scotiae sp. nov. The type strain of Actinobacillus scotiae sp. nov. is NCTC 12922T(= M2000/95/1T).
Ravi Naidu - One of the best experts on this subject based on the ideXlab platform.
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Hydrolysis of fenamiphos and its toxic oxidation products by MicroBacterium sp. in pure culture and groundwater.
Bioresource technology, 2009Co-Authors: Tanya Cáceres, Mallavarapu Megharaj, Seidu Malik, Michael Beer, Ravi NaiduAbstract:A Bacterium with an exceptional ability to hydrolyse fenamiphos and its toxic oxidation products fenamiphos sulfoxide and fenamiphos sulfone, all possessing POC bond was isolated from soil. Based on 16S rRNA gene determination, this Bacterium was putatively identified as MicroBacterium esteraromaticum. The phenols (fenamiphos phenol, sulfoxide phenol and sulfone phenol) formed during bacterial hydrolysis resisted further degradation in mineral salts medium and sterile groundwater, but were transitory in non-sterile groundwater due to the catabolism of native microorganisms. Also, the cell-free preparation of this Bacterium was highly effective in hydrolysing fenamiphos and its oxides. These results demonstrate the potential of this Bacterium to detoxify pesticide waste in the environment including the groundwater.
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Bioavailability of an organophosphorus pesticide, fenamiphos, sorbed on an organo clay.
Journal of agricultural and food chemistry, 2003Co-Authors: Neera Singh, Mallavarapu Megharaj, Ravi Naidu, Rai S Kookana, Will P. Gates, Gordon Jock Churchman, Jenny S. Anderson, Zuliang Chen, Phil G. Slade, N SethunathanAbstract:Hydrolysis of an insecticide/nematicide, fenamiphos [ethyl-3-methyl-4-(methylthio)phenyl-(1-methylethyl)phosphoramidate], immobilized through sorption by cetyltrimethylammonium-exchanged montmorillonite (CTMA-clay) by a soil Bacterium, BreviBacterium sp., was examined. X-ray diffraction analysis, infrared spectra, and a negative electrophoretic mobility strongly indicated that fenamiphos was intercalated within the bacterially inaccessible interlayer spaces of CTMA-clay. The Bacterium hydrolyzed, within 24 h, 82% of the fenamiphos sorbed by the CTMA-clay complex. There was a concomitant accumulation of hydrolysis product, fenamiphos phenol, in nearly stoichiometric amounts. During the same period, in abiotic (uninoculated) controls, 4.6% of the sorbed insecticide was released into the aqueous phase as compared to 6.0% of the sorbed fenamiphos in another abiotic control where activated carbon, a sink for desorbed fenamiphos, was present. Thus, within 24 h, the Bacterium hydrolyzed 77% more fenamiphos sorbed by organo clay than the amounts desorbed in abiotic controls. Such rapid degradation of an intercalated pesticide by a Bacterium has not been reported before. Evidence indicated that extracellular enzymes produced by the Bacterium rapidly hydrolyzed the nondesorbable fenamiphos, even when the enzyme itself was sorbed. Fenamiphos strongly sorbed to an organo clay appears to be readily available for exceptionally rapid degradation by the Bacterium.
Roger A. Hutson - One of the best experts on this subject based on the ideXlab platform.
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phocoenobacter uteri gen nov sp nov a new member of the family pasteurellaceae pohl 1979 1981 isolated from a harbour porpoise phocoena phocoena
International Journal of Systematic and Evolutionary Microbiology, 2000Co-Authors: Geoffrey Foster, Roger A. Hutson, H M Ross, Henry Malnick, Anne Willems, R J Reid, M D CollinsAbstract:Phenotypic and phylogenetic studies were performed on a Gram-negative, rod-shaped Bacterium isolated from the uterus of a porpoise. Biochemical and physiological studies indicated that the Bacterium was related to the family Pasteurellaceae. Comparative 16S rRNA gene sequencing studies confirmed these findings and demonstrated that the Bacterium represents a hitherto unknown subline within this family of organisms. Based on the results of the phylogenetic analysis and phenotypic criteria, it is proposed that the Bacterium be assigned to a new genus, Phocoenobacter uteri gen. nov., sp. nov. The type strain of Phocoenobacter uteri sp. nov. is NCTC 12872T.
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Arthrobacter rhombi sp. nov., isolated from Greenland halibut (Reinhardtius hippoglossoides).
International Journal of Systematic and Evolutionary Microbiology, 1999Co-Authors: Carlos R. Osorio, Juan L. Barja, Roger A. Hutson, Matthew D. CollinsAbstract:Two strains of a hitherto undescribed Gram-positive coryneform Bacterium isolated from Greenland halibut (Reinhardtius hippoglossoides) were characterized by phenotypic and molecular taxonomic methods. Comparative 16S rRNA gene sequencing studies demonstrated that the unknown strains constitute a new line within the genus Arthrobacter. The nearest relatives of the Bacterium from fish were members of the Arthrobacter nicotianael Arthrobacter sulfureus group. The unknown Bacterium was readily distinguished from these species by phenotypic methods. Based on phylogenetic and phenotypic evidence, it is proposed that the unknown Bacterium be classified as Arthrobacter rhombi sp. nov. The type strain of Arthrobacter rhombi is CCUG 38813T.
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actinobacillus scotiae sp nov a new member of the family pasteurellaceae pohl 1979 1981 isolated from porpoises phocoena phocoena
International Journal of Systematic and Evolutionary Microbiology, 1998Co-Authors: Geoffrey Foster, Roger A. Hutson, H M Ross, I A P Patterson, M D CollinsAbstract:Phenotypic and phylogenetic studies were performed on a Gram-negative, rod-shaped Bacterium isolated from three porpoises. Biochemical and physiological studies indicated that the Bacterium was related to the family Pasteurellaceae. Comparative 16S rRNA gene sequencing studies confirmed these findings and demonstrated that the Bacterium represents a hitherto unknown subline. The nearest phylogenetic relative of the unknown Bacterium wasActinobacillus delphinicola, an organism also originating from sea mammals, although a sequence divergence of 3% demonstrated that the newly isolated Bacterium is a distinct species. On the basis of the results of the phylogenetic analysis and phenotypic criteria, it is proposed that the Bacterium should be classified as a new species, Actinobacillus scotiae sp. nov. The type strain of Actinobacillus scotiae sp. nov. is NCTC 12922T(= M2000/95/1T).
Wang N - One of the best experts on this subject based on the ideXlab platform.
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The influence of the state of Bacterium suspension on the acute toxicity test using fresh luminescent Bacterium Vibrio Qinghaiensis sp.-Q67
Journal of Xi'an University of Architecture & Technology, 2014Co-Authors: Wang NAbstract:ZnSO4·7H2O, CdCl2·2.5H2 O, trichloroisocyanuric acid and sodium dodecyl benzene sulfonate were selected to detect the impact about the state of Bacterium suspension on the reproducibility of acute toxicity tests with luminescent Bacterium Vibrio Qinghaiensis sp.-Q67 as test Bacterium. The great variations of the toxicity of the four chemicals on Q67 were observed, using either the Bacterium suspension which was cultivated for 8.5 h with different storage time of strains or the same storage time of strains which was cultivated for 8.5, 9.0 and 10 hrs respectively. The parameters of Q67 suspension, such as pH, relative light unit(RLU) and bacterial density(OD600), were detected to discuss the reasons of the great variations obtained in the results. It showed that there was slight distinction in the pH of Bacterium suspension at different growth stage but significant changes of the OD600 value and RLU value which showed their significant influence on the reproducibility of the tests. Since the OD600 could affect the RLU value of Bacterium suspension, it is necessary to identify the OD600 of Q67 Bacterium suspension in order to obtain the good reproducibility of acute toxicity test.
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Effect of the Bacterium suspension density on the acute toxicity tests with the fresh luminescent Bacterium Vibrio qinghaiensis sp.-Q67
Applied Mechanics and Materials, 2014Co-Authors: Wang NAbstract:This paperis inclined to identify and evaluate the effects of the Bacterium suspension density on the acute toxicity tests with the fresh luminescent Bacterium Vibrio qinghaiensis sp.-Q67,which has been widely used for detecting the acute toxicity of the environment.However,for the time being,it is far being satisfactory to use the fresh cultured Q67 Bacterium suspension as the test Bacterium for reproducibility.Itis justfor this reason thatwe have been attempting to use the absorbance of UV spectrophotometer at 600 nm(OD600)for the density of Bacterium suspension.Cherishing thisgoal inmind,we have,firstly,studied the growth curve(OD600-time)of Q67 strains with different storing time lengths.Secondly,we have measured the luminous intensity by using the microplate luminometer for the acute toxicity tests with five different chemical substances,including ZnSO4 ·7H2 O,CdCl2 ·2.5H2 O.In the above said tests,we have also conducted the testswith flusilazole,SDBS and TCCA,alongwith the oil-field sewage,electroplating sewage and coking sewage on the Q67 in the middle and late growth stage with differentkinds of OD600.The growth curve indicates that it is difficult to use the culturing time to control the growth state of Q67 since the storage time has a great influence on the growth speed of Q67,while the density of Bacterium suspension is a good indicator to determine the state of bacteria.In addition,the results of the acute toxicity tests are greatly influenced by the bacteria density,the EC 50 values of all the samples are much higher than in the other growth stages during the OD600 value between 1.6-1.8.Some kinds of the industrial sewage in the low concentration have strong stimulating effects on Q67 during the OD600 value around 2.5,but they may have a certain-degree inhibition effect on Q67 when the OD600 value is between 1.6-2.0.The sensitivity of the luminescent Bacterium toxicity test is the highest when the OD600 of Q67 Bacterium suspension is about 2.0.Furthermore,a good reproducibility will be obtainedwhile the OD600 of Bacterium suspension remains constant,then itwould be possible to eliminate the influence of the amount of inoculation and the storage time of Bacterium.
