The Experts below are selected from a list of 1053 Experts worldwide ranked by ideXlab platform
Luiz Antonio Sangioni - One of the best experts on this subject based on the ideXlab platform.
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prevalencia do Balantidium coli nas fezes de suinos na fase de terminacao em abatedouro
Agrarian, 2010Co-Authors: Renata Pâmela Barrachini Steffen, Kelly Daiane Soares, Lucas Pedroso Colvero, Rodrigo Garofallo Garcia, Luiz Antonio SangioniAbstract:O objetivo deste trabalho foi avaliar a presenca do protozoario Balantidium coli em suinos de terminacao em um abatedouro da regiao central do estado do Rio Grande do Sul por meio de exame coproparasitologico, tecnica de Faust – modificado. Foram coletadas 287 amostras sendo que destas, em 157 foi detectado pelo menos um cisto de B. coli , totalizando uma prevalencia de 54,7%. Alem disso, houve variacoes entre os diferentes lotes coletados de 25,22 a 93,65%. Essa alta prevalencia encontrada ressalta a importância de estudos futuros com relacao ao envolvimento da infeccao deste protozoario na producao dos suinos assim como da relevância desta infeccao do ponto de vista da saude publica.
Ricardo Atencio - One of the best experts on this subject based on the ideXlab platform.
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balantidiasis en una nina indigena de la sierra de perija venezuela reporte de un caso
Kasmera, 2006Co-Authors: Rosita Chengng, S Dorfman, Raimy Mindiola, Francis Villarroel, Odelis Diazsuarez, Ricardo AtencioAbstract:Se reporta el caso de una paciente indigena de la etnia Yucpa de la Sierra de Perija-Venezuela, con cuadro de diarrea por Balantidium coli. El diagnostico se realizo mediante el examen directo de las heces. Se le indico tratamiento con Metronidazol. La evolucion fue satisfactoria.
Maria Regina Reis Amendoeira - One of the best experts on this subject based on the ideXlab platform.
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comparative study of three xenic media culture for cultivation of Balantidium coli strains
Revista Brasileira De Parasitologia Veterinaria, 2018Co-Authors: Alynne Da Silva Barbosa, Matheus Lessa Cardozo, Laís Verdan Dib, Ana Beatriz Monteiro Fonseca, Claudia Maria Antunes Uchôa, Otilio Machado Pereira Bastos, Maria Regina Reis AmendoeiraAbstract:The aim of the present study was to evaluate the growth rate of Balantidium coli in three xenic media cultures. Between 2013 and 2015, 10 B. coli isolates obtained from feces of Cynomolgus macaques, and 30 isolates from feces of pigs were studied. An inoculum of 500 trophozoites was transferred to tubes containing LES, TYSGM-9 and Pavlova media. These cultures were evaluated at incubation times of 24, 48, 72 and 96 hours. In most of strains analyzed wasn't showed significant difference in the growth rate comparing TYSGM-9 and Pavlova media (Wilcoxon p>0.016). In Pavlova medium, the trophozoites showed a maximum growth at 72 hours with significant difference when compared with the times of 24 h and 96 h (Wilcoxon <0.008). In LES, viable trophozoites were observed until 24 hours, with a significant difference (Friedman p<0.05, Wilcoxon p<0.016) in the number of parasite cells compared with Pavlova and TYSGM-9 media cultures. Thus, LES medium seemed to be less adequate than the other media for maintenance of B. coli. Despite the satisfactory results in TYSGM-9, Pavlova medium was considered ideal for the maintenance of this protozoan strain, guaranteeing the viability of the parasite with subculture every three days, presenting lower costs.
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Comparative study of three xenic media culture for cultivation of Balantidium coli strains
2018Co-Authors: Alynne Da Silva Barbosa, Matheus Lessa Cardozo, Laís Verdan Dib, Ana Beatriz Monteiro Fonseca, Claudia Maria Antunes Uchôa, Otilio Machado Pereira Bastos, Maria Regina Reis AmendoeiraAbstract:Abstract The aim of the present study was to evaluate the growth rate of Balantidium coli in three xenic media cultures. Between 2013 and 2015, 10 B. coli isolates obtained from feces of Cynomolgus macaques, and 30 isolates from feces of pigs were studied. An inoculum of 500 trophozoites was transferred to tubes containing LES, TYSGM-9 and Pavlova media. These cultures were evaluated at incubation times of 24, 48, 72 and 96 hours. In most of strains analyzed wasn’t showed significant difference in the growth rate comparing TYSGM-9 and Pavlova media (Wilcoxon p>0.016). In Pavlova medium, the trophozoites showed a maximum growth at 72 hours with significant difference when compared with the times of 24 h and 96 h (Wilcoxon
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avaliacao da frequencia de Balantidium coli em suinos tratadores de suinos e primatas nao humanos no estado do rio de janeiro
Revista de Patologia Tropical, 2016Co-Authors: Alynne Da Silva Barbosa, Laís Verdan Dib, Claudia Maria Antunes Uchôa, Otilio Machado Pereira Bastos, Maria Regina Reis AmendoeiraAbstract:This paper reports the frequency of Balantidium coli in pig, pig handlers and nonhuman primate working on farms located in different municipalities in the state of Rio de Janeiro. From 2012 to 2014, 104 fecal samples were collected from 52 handlers, 790 fecal samples from pigs and 1,115 from nonhuman primates. All samples were collected free of chemical preservatives and then underwent the following techniques: Faust et al., Sheather modified, Ritchie modified and Lutz, as well as direct examination. Evolutionary forms of B. coli were not detected in the fecal samples from the handlers, but were found in 49.2% of the fecal material from pigs, and in 41.5%. from apes. The absence of balantidiosis in humans, in spite of being in close contact with infected animals, as demonstrated in this study, may be due to the adoption of adequate sanitary measures for handling the animals or even due to human resistance to infection by this parasite.
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isolation and maintenance of Balantidium coli malmsteim 1857 cultured from fecal samples of pigs and non human primates
Veterinary Parasitology, 2015Co-Authors: Alynne Da Silva Barbosa, Matheus Lessa Cardozo, Laís Verdan Dib, Claudia Maria Antunes Uchôa, Otilio Machado Pereira Bastos, Alcides Pissinatti, Eduarda Peixoto Azevedo, Mayara Perlingeiro De Siqueira, Paulo Ricardo Ferreira Filho, Maria Regina Reis AmendoeiraAbstract:Balantidium coli is a protozoa that can determine dysentery in humans, pigs and non-human primates having zoonotic potential. The lack of standardization in isolation and maintenance hinders the development of research on its biology and epidemiology. This study is aimed to standardize the isolation and maintenance of this parasite from animal feces, in culture medium, Pavlova modified. From 2012 to 2014, 1905 fecal samples were collected from captive animals of Rio de Janeiro. Were selected for isolation samples with a minimum of 10 trophozoites and/or 30 cysts of B. coli, totaling 88 pigs, 26 Cynomolgus and 90 rhesus macaques. In the presence of cysts, the sample was homogenized in saline solution, 500 μL was removed and inoculated into culture medium. The material that contained trophozoites the inoculum was made from 240 μL of fecal solution. All inoculate tubes with the subcultures were kept at 36°C, and sterile rice starch was always added to the medium. The parasites isolate from pigs, 34%, and from Cynomolgus 38.4% were maintained in vitro for a period of more than 24 months. These procedures proved to be adequate for isolation and maintenance of B. coli from different animals, they were found to be inexpensive and easy to perform.
Alynne Da Silva Barbosa - One of the best experts on this subject based on the ideXlab platform.
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comparative study of three xenic media culture for cultivation of Balantidium coli strains
Revista Brasileira De Parasitologia Veterinaria, 2018Co-Authors: Alynne Da Silva Barbosa, Matheus Lessa Cardozo, Laís Verdan Dib, Ana Beatriz Monteiro Fonseca, Claudia Maria Antunes Uchôa, Otilio Machado Pereira Bastos, Maria Regina Reis AmendoeiraAbstract:The aim of the present study was to evaluate the growth rate of Balantidium coli in three xenic media cultures. Between 2013 and 2015, 10 B. coli isolates obtained from feces of Cynomolgus macaques, and 30 isolates from feces of pigs were studied. An inoculum of 500 trophozoites was transferred to tubes containing LES, TYSGM-9 and Pavlova media. These cultures were evaluated at incubation times of 24, 48, 72 and 96 hours. In most of strains analyzed wasn't showed significant difference in the growth rate comparing TYSGM-9 and Pavlova media (Wilcoxon p>0.016). In Pavlova medium, the trophozoites showed a maximum growth at 72 hours with significant difference when compared with the times of 24 h and 96 h (Wilcoxon <0.008). In LES, viable trophozoites were observed until 24 hours, with a significant difference (Friedman p<0.05, Wilcoxon p<0.016) in the number of parasite cells compared with Pavlova and TYSGM-9 media cultures. Thus, LES medium seemed to be less adequate than the other media for maintenance of B. coli. Despite the satisfactory results in TYSGM-9, Pavlova medium was considered ideal for the maintenance of this protozoan strain, guaranteeing the viability of the parasite with subculture every three days, presenting lower costs.
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Comparative study of three xenic media culture for cultivation of Balantidium coli strains
2018Co-Authors: Alynne Da Silva Barbosa, Matheus Lessa Cardozo, Laís Verdan Dib, Ana Beatriz Monteiro Fonseca, Claudia Maria Antunes Uchôa, Otilio Machado Pereira Bastos, Maria Regina Reis AmendoeiraAbstract:Abstract The aim of the present study was to evaluate the growth rate of Balantidium coli in three xenic media cultures. Between 2013 and 2015, 10 B. coli isolates obtained from feces of Cynomolgus macaques, and 30 isolates from feces of pigs were studied. An inoculum of 500 trophozoites was transferred to tubes containing LES, TYSGM-9 and Pavlova media. These cultures were evaluated at incubation times of 24, 48, 72 and 96 hours. In most of strains analyzed wasn’t showed significant difference in the growth rate comparing TYSGM-9 and Pavlova media (Wilcoxon p>0.016). In Pavlova medium, the trophozoites showed a maximum growth at 72 hours with significant difference when compared with the times of 24 h and 96 h (Wilcoxon
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avaliacao da frequencia de Balantidium coli em suinos tratadores de suinos e primatas nao humanos no estado do rio de janeiro
Revista de Patologia Tropical, 2016Co-Authors: Alynne Da Silva Barbosa, Laís Verdan Dib, Claudia Maria Antunes Uchôa, Otilio Machado Pereira Bastos, Maria Regina Reis AmendoeiraAbstract:This paper reports the frequency of Balantidium coli in pig, pig handlers and nonhuman primate working on farms located in different municipalities in the state of Rio de Janeiro. From 2012 to 2014, 104 fecal samples were collected from 52 handlers, 790 fecal samples from pigs and 1,115 from nonhuman primates. All samples were collected free of chemical preservatives and then underwent the following techniques: Faust et al., Sheather modified, Ritchie modified and Lutz, as well as direct examination. Evolutionary forms of B. coli were not detected in the fecal samples from the handlers, but were found in 49.2% of the fecal material from pigs, and in 41.5%. from apes. The absence of balantidiosis in humans, in spite of being in close contact with infected animals, as demonstrated in this study, may be due to the adoption of adequate sanitary measures for handling the animals or even due to human resistance to infection by this parasite.
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comparison of five parasitological techniques for laboratory diagnosis of Balantidium coli cysts
Revista Brasileira De Parasitologia Veterinaria, 2016Co-Authors: Alynne Da Silva Barbosa, Laís Verdan Dib, Claudia Maria Antunes Uchôa, Otilio Machado Pereira Bastos, Alcides Pissinatti, Augusto Cesar Machado Pereira Bastos, Igo Vieira De Souza, Eduarda Peixoto Azevedo, Mayara Perlingeiro De Siqueira, Matheus Lessa CardozoAbstract:Balantidium coli is a protozoon that can cause dysentery in humans, pigs and nonhuman primates, with zoonotic potential. In the literature, there is still little information on the effectiveness of different laboratory techniques for diagnosing this disease. This study compared and evaluated the performance of the Lutz, modified Ritchie, Faust, modified Sheather and direct examination techniques for detecting cysts of this protozoon. Between 2012 and 2014, 1905 fecal samples were collected from captive animals in the state of Rio de Janeiro. Of these, 790 were obtained from the rectum of pigs and 1115 from enclosures occupied by nonhuman primates. B. coli cysts were most evident through direct examination (22.4% of the samples) and the Lutz technique (21%). Fair agreement (Kappa = 0.41; p < 0.05) was observed only between direct examination and Lutz. The flotation techniques (Faust and modified Sheather) did not show good recovery of cysts. A statistically significant difference (p < 0.05) in the frequency of cysts between pigs and nonhuman primates could only be observed through direct examination and the Lutz technique. The most efficient method for diagnosing this parasitosis was seen to an association between direct examination and the spontaneous sedimentation technique.
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isolation and maintenance of Balantidium coli malmsteim 1857 cultured from fecal samples of pigs and non human primates
Veterinary Parasitology, 2015Co-Authors: Alynne Da Silva Barbosa, Matheus Lessa Cardozo, Laís Verdan Dib, Claudia Maria Antunes Uchôa, Otilio Machado Pereira Bastos, Alcides Pissinatti, Eduarda Peixoto Azevedo, Mayara Perlingeiro De Siqueira, Paulo Ricardo Ferreira Filho, Maria Regina Reis AmendoeiraAbstract:Balantidium coli is a protozoa that can determine dysentery in humans, pigs and non-human primates having zoonotic potential. The lack of standardization in isolation and maintenance hinders the development of research on its biology and epidemiology. This study is aimed to standardize the isolation and maintenance of this parasite from animal feces, in culture medium, Pavlova modified. From 2012 to 2014, 1905 fecal samples were collected from captive animals of Rio de Janeiro. Were selected for isolation samples with a minimum of 10 trophozoites and/or 30 cysts of B. coli, totaling 88 pigs, 26 Cynomolgus and 90 rhesus macaques. In the presence of cysts, the sample was homogenized in saline solution, 500 μL was removed and inoculated into culture medium. The material that contained trophozoites the inoculum was made from 240 μL of fecal solution. All inoculate tubes with the subcultures were kept at 36°C, and sterile rice starch was always added to the medium. The parasites isolate from pigs, 34%, and from Cynomolgus 38.4% were maintained in vitro for a period of more than 24 months. These procedures proved to be adequate for isolation and maintenance of B. coli from different animals, they were found to be inexpensive and easy to perform.
Renata Pâmela Barrachini Steffen - One of the best experts on this subject based on the ideXlab platform.
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prevalencia do Balantidium coli nas fezes de suinos na fase de terminacao em abatedouro
Agrarian, 2010Co-Authors: Renata Pâmela Barrachini Steffen, Kelly Daiane Soares, Lucas Pedroso Colvero, Rodrigo Garofallo Garcia, Luiz Antonio SangioniAbstract:O objetivo deste trabalho foi avaliar a presenca do protozoario Balantidium coli em suinos de terminacao em um abatedouro da regiao central do estado do Rio Grande do Sul por meio de exame coproparasitologico, tecnica de Faust – modificado. Foram coletadas 287 amostras sendo que destas, em 157 foi detectado pelo menos um cisto de B. coli , totalizando uma prevalencia de 54,7%. Alem disso, houve variacoes entre os diferentes lotes coletados de 25,22 a 93,65%. Essa alta prevalencia encontrada ressalta a importância de estudos futuros com relacao ao envolvimento da infeccao deste protozoario na producao dos suinos assim como da relevância desta infeccao do ponto de vista da saude publica.
