The Experts below are selected from a list of 177 Experts worldwide ranked by ideXlab platform
Freddy Haesebrouck - One of the best experts on this subject based on the ideXlab platform.
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In-feed Bambermycin medication induces anti-inflammatory effects and prevents parietal cell loss without influencing Helicobacter suis colonization in the stomach of mice
Veterinary Research, 2018Co-Authors: Chloë De Witte, Bernard Taminiau, Bram Flahou, Veerle Hautekiet, Georges Daube, Richard Ducatelle, Freddy HaesebrouckAbstract:AbstractThe minimum inhibitory concentration of Bambermycin on three porcine Helicobacter suis strains was shown to be 8 μg/mL. The effect of in-feed medication with this antibiotic on the course of a gastric infection with one of these strains, the host response and the gastric microbiota was determined in mice, as all of these parameters may be involved in gastric pathology. In H. suis infected mice which were not treated with Bambermycin, an increased number of infiltrating B-cells, T-cells and macrophages in combination with a Th2 response was demonstrated, as well as a decreased parietal cell mass. Compared to this non-treated, infected group, in H. suis infected mice medicated with Bambermycin, gastric H. suis colonization was not altered, but a decreased number of infiltrating T-cells, B-cells and macrophages as well as downregulated expressions of IL-1β, IL-8M, IL-10 and IFN-γ were demonstrated and the parietal cell mass was not affected. In Bambermycin treated mice that were not infected with H. suis, the number of infiltrating T-cells and expression of IL-1β were lower than in non-infected mice that did not receive Bambermycin. Gastric microbiota analysis indicated that the relative abundance of bacteria that might exert unfavorable effects on the host was decreased during Bambermycin supplementation. In conclusion, Bambermycin did not affect H. suis colonization, but decreased gastric inflammation and inhibited the effects of a H. suis infection on parietal cell loss. Not only direct interaction of H. suis with parietal cells, but also inflammation may play a role in death of these gastric acid producing cells.
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MOESM7 of In-feed Bambermycin medication induces anti-inflammatory effects and prevents parietal cell loss without influencing Helicobacter suis colonization in the stomach of mice
2018Co-Authors: Chloë De Witte, Bernard Taminiau, Bram Flahou, Veerle Hautekiet, Georges Daube, Richard Ducatelle, Freddy HaesebrouckAbstract:Additional file 7. Overview of the number of pyrosequencing reads for each mice. Group 1 = H. suis-negative control group without Bambermycin supplementation; group 2 = 32 ppm Bambermycin supplemented, non-H. suis infected group; group 3 = 64 ppm Bambermycin supplemented, non-H. suis infected group; group 4 = H. suis-positive control group without Bambermycin supplementation; group 5 = 32 ppm Bambermycin supplemented, H. suis infected group; group 6 = 64 ppm Bambermycin supplemented, H. suis infected group
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MOESM9 of In-feed Bambermycin medication induces anti-inflammatory effects and prevents parietal cell loss without influencing Helicobacter suis colonization in the stomach of mice
2018Co-Authors: Chloë De Witte, Bernard Taminiau, Bram Flahou, Veerle Hautekiet, Georges Daube, Richard Ducatelle, Freddy HaesebrouckAbstract:Additional file 9. Overview of the gastric bacterial richness, diversity and evenness of the Bambermycin-supplemented and non-supplemented groups. Gastric bacterial richness (A), diversity (B) and evenness (C). The data are represented as box plots: the bottom and top of the box represent the first and the third quartile, the line in the box represents the median and the whiskers represent the minimum and maximum values. Group 1 = H. suis-negative control group without Bambermycin supplementation; group 2 = 32 ppm Bambermycin supplemented, non-H. suis infected group; group 3 = 64 ppm Bambermycin supplemented, non-H. suis infected group; group 4 = H. suis-positive control group without Bambermycin supplementation; group 5 = 32 ppm Bambermycin supplemented, H. suis infected group; group 6 = 64 ppm Bambermycin supplemented, H. suis infected group
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MOESM5 of In-feed Bambermycin medication induces anti-inflammatory effects and prevents parietal cell loss without influencing Helicobacter suis colonization in the stomach of mice
2018Co-Authors: Chloë De Witte, Bernard Taminiau, Bram Flahou, Veerle Hautekiet, Georges Daube, Richard Ducatelle, Freddy HaesebrouckAbstract:Additional file 5. Overview of the relative fold changes of altered markers for inflammation in the Bambermycin-supplemented and non-supplemented groups. The data are presented as fold changes in gene expression normalized to 3 reference genes and relative to control groups 1 and 4 (i.e. group 2-4 relative to group 1 and group 5-6 relative to group 4) which are considered as 1. The fold changes are shown as means with the standard error of the mean. Statistical differences were calculated using the non-parametric Kruskal–Wallis H test SPSS statistics 24®. A P-value lower than 0.05 is considered to be significant. Group 2 = 32 ppm Bambermycin supplemented, non-H. suis infected group; group 3 = 64 ppm Bambermycin supplemented, non-H. suis infected group; group 4 = H. suis-positive control group without Bambermycin supplementation; group 5 = 32 ppm Bambermycin supplemented, H. suis infected group; group 6 = 64 ppm Bambermycin supplemented, H. suis infected group
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MOESM6 of In-feed Bambermycin medication induces anti-inflammatory effects and prevents parietal cell loss without influencing Helicobacter suis colonization in the stomach of mice
2018Co-Authors: Chloë De Witte, Bernard Taminiau, Bram Flahou, Veerle Hautekiet, Georges Daube, Richard Ducatelle, Freddy HaesebrouckAbstract:Additional file 6. Overview of the relative fold changes of altered markers for gastric acid secretion in the Bambermycin-supplemented and non-supplemented groups. The data are presented as fold changes in gene expression normalized to 3 reference genes and relative to control groups 1 and 4 (i.e. group 2-4 relative to group 1 and group 5-6 relative to group 4) which are considered as 1. The fold changes are shown as means with the standard error of the mean. Statistical differences were calculated using the non-parametric Kruskal–Wallis H test SPSS statistics 24®. A P-value lower than 0.05 is considered to be significant. Group 2 = 32 ppm Bambermycin supplemented, non-H. suis infected group; group 3 = 64 ppm Bambermycin supplemented, non-H. suis infected group; group 4 = H. suis-positive control group without Bambermycin supplementation; group 5 = 32 ppm Bambermycin supplemented, H. suis infected group; group 6 = 64 ppm Bambermycin supplemented, H. suis infected group
David J Nisbet - One of the best experts on this subject based on the ideXlab platform.
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effects of the antibiotic ionophores monensin lasalocid laidlomycin propionate and Bambermycin on salmonella and e coli o157 h7 in vitro
Journal of Applied Microbiology, 2003Co-Authors: Thomas S. Edrington, T R Callaway, P D Varey, R O Elder, Elizabeth Kutter, Andrew D Brabban, Kenneth M. Bischoff, Y. S. Jung, Robin C Anderson, David J NisbetAbstract:T.S. E DRINGTON, T .R. C ALLAWAY, P .D. V AREY, Y .S. J UNG, K .M. B ISCHOFF, R .O. E LDER, R . C . A N D E R S O N , E . K U T T E R , A . D . B R A B B A N A N D D . J . N I S B E T . 2003. Aims: To examine the effects of ionophores on Salmonella and Escherichia coli O157:H7 in pure and mixed ruminal fluid cultures. Methods and Results: Four Salmonella serotypes (Dublin, Derby, Typhimurium, and Enteriditis) and two strains of E. coli O157:H7 (ATCC 43895 and FDIU 6058) were cultured in the presence of varying concentrations of ionophores (monensin, lasalocid, laidlomycin propionate, and Bambermycin) in pure and mixed ruminal fluid cultures. Bacterial growth rates in pure culture were not affected (P >0 AE10) by ionophores at concentrations up to 10 times the approximate rumen ionophore concentration under normal feeding regimens. Likewise, ionophores had no effect (P >0 AE10) on Salmonella or E. coli CFU plated from 24-h ruminal fluid incubations. Ionophore treatment decreased (P <0 AE01) the acetate : propionate ratio in ruminal fluid cultures as expected. Conclusions: Ionophores had no effect on the foodborne pathogens Salmonella and E. coli O157:H7 in vitro. Significance and Impact of the Study: The results suggest that ionophore feeding would have little or no effect on Salmonella or E. coli populations in the ruminant.
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effect of feeding the ionophores monensin and laidlomycin propionate and the antimicrobial Bambermycin to sheep experimentally infected with e coli o157 h7 and salmonella typhimurium
Journal of Animal Science, 2003Co-Authors: Thomas S. Edrington, T R Callaway, R O Elder, Kenneth M. Bischoff, Robin C Anderson, Kenneth J Genovese, David J NisbetAbstract:Escherichia coli O157:H7 and Salmo- nella are widely recognized as important agents of food- borne disease with worldwide distribution. The use of ionophores in feeding growing ruminants is widespread in the United States and has attracted recent interest due to the apparent temporal relationship between ini- tial ionophore use and the increase in human E. coli O157:H7 cases. Two experiments were conducted to evaluate the effects of short-term feeding of ionophores on fecal shedding, intestinal concentrations, and anti- microbial susceptibility of E. coli O157:H7 and S. typhi- murium in growing lambs. Sixteen lambs were used in each experiment, four lambs per treatment group: monensin, laidlomycin propionate, Bambermycin, and a control treatment. Lambs were fed a grain and hay (50:50) diet with their respective ionophore for 12 d before experimental inoculation with E. coli O157:H7
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Effects of the antibiotic ionophores monensin, lasalocid, laidlomycin propionate and Bambermycin on Salmonella and E. coli O157:H7 in vitro*†
Journal of applied microbiology, 2003Co-Authors: Thomas S. Edrington, T R Callaway, P D Varey, R O Elder, Elizabeth Kutter, Andrew D Brabban, Kenneth M. Bischoff, Y. S. Jung, Robin C Anderson, David J NisbetAbstract:T.S. E DRINGTON, T .R. C ALLAWAY, P .D. V AREY, Y .S. J UNG, K .M. B ISCHOFF, R .O. E LDER, R . C . A N D E R S O N , E . K U T T E R , A . D . B R A B B A N A N D D . J . N I S B E T . 2003. Aims: To examine the effects of ionophores on Salmonella and Escherichia coli O157:H7 in pure and mixed ruminal fluid cultures. Methods and Results: Four Salmonella serotypes (Dublin, Derby, Typhimurium, and Enteriditis) and two strains of E. coli O157:H7 (ATCC 43895 and FDIU 6058) were cultured in the presence of varying concentrations of ionophores (monensin, lasalocid, laidlomycin propionate, and Bambermycin) in pure and mixed ruminal fluid cultures. Bacterial growth rates in pure culture were not affected (P >0 AE10) by ionophores at concentrations up to 10 times the approximate rumen ionophore concentration under normal feeding regimens. Likewise, ionophores had no effect (P >0 AE10) on Salmonella or E. coli CFU plated from 24-h ruminal fluid incubations. Ionophore treatment decreased (P
Thomas S. Edrington - One of the best experts on this subject based on the ideXlab platform.
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effects of the antibiotic ionophores monensin lasalocid laidlomycin propionate and Bambermycin on salmonella and e coli o157 h7 in vitro
Journal of Applied Microbiology, 2003Co-Authors: Thomas S. Edrington, T R Callaway, P D Varey, R O Elder, Elizabeth Kutter, Andrew D Brabban, Kenneth M. Bischoff, Y. S. Jung, Robin C Anderson, David J NisbetAbstract:T.S. E DRINGTON, T .R. C ALLAWAY, P .D. V AREY, Y .S. J UNG, K .M. B ISCHOFF, R .O. E LDER, R . C . A N D E R S O N , E . K U T T E R , A . D . B R A B B A N A N D D . J . N I S B E T . 2003. Aims: To examine the effects of ionophores on Salmonella and Escherichia coli O157:H7 in pure and mixed ruminal fluid cultures. Methods and Results: Four Salmonella serotypes (Dublin, Derby, Typhimurium, and Enteriditis) and two strains of E. coli O157:H7 (ATCC 43895 and FDIU 6058) were cultured in the presence of varying concentrations of ionophores (monensin, lasalocid, laidlomycin propionate, and Bambermycin) in pure and mixed ruminal fluid cultures. Bacterial growth rates in pure culture were not affected (P >0 AE10) by ionophores at concentrations up to 10 times the approximate rumen ionophore concentration under normal feeding regimens. Likewise, ionophores had no effect (P >0 AE10) on Salmonella or E. coli CFU plated from 24-h ruminal fluid incubations. Ionophore treatment decreased (P <0 AE01) the acetate : propionate ratio in ruminal fluid cultures as expected. Conclusions: Ionophores had no effect on the foodborne pathogens Salmonella and E. coli O157:H7 in vitro. Significance and Impact of the Study: The results suggest that ionophore feeding would have little or no effect on Salmonella or E. coli populations in the ruminant.
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effect of feeding the ionophores monensin and laidlomycin propionate and the antimicrobial Bambermycin to sheep experimentally infected with e coli o157 h7 and salmonella typhimurium
Journal of Animal Science, 2003Co-Authors: Thomas S. Edrington, T R Callaway, R O Elder, Kenneth M. Bischoff, Robin C Anderson, Kenneth J Genovese, David J NisbetAbstract:Escherichia coli O157:H7 and Salmo- nella are widely recognized as important agents of food- borne disease with worldwide distribution. The use of ionophores in feeding growing ruminants is widespread in the United States and has attracted recent interest due to the apparent temporal relationship between ini- tial ionophore use and the increase in human E. coli O157:H7 cases. Two experiments were conducted to evaluate the effects of short-term feeding of ionophores on fecal shedding, intestinal concentrations, and anti- microbial susceptibility of E. coli O157:H7 and S. typhi- murium in growing lambs. Sixteen lambs were used in each experiment, four lambs per treatment group: monensin, laidlomycin propionate, Bambermycin, and a control treatment. Lambs were fed a grain and hay (50:50) diet with their respective ionophore for 12 d before experimental inoculation with E. coli O157:H7
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Effects of the antibiotic ionophores monensin, lasalocid, laidlomycin propionate and Bambermycin on Salmonella and E. coli O157:H7 in vitro*†
Journal of applied microbiology, 2003Co-Authors: Thomas S. Edrington, T R Callaway, P D Varey, R O Elder, Elizabeth Kutter, Andrew D Brabban, Kenneth M. Bischoff, Y. S. Jung, Robin C Anderson, David J NisbetAbstract:T.S. E DRINGTON, T .R. C ALLAWAY, P .D. V AREY, Y .S. J UNG, K .M. B ISCHOFF, R .O. E LDER, R . C . A N D E R S O N , E . K U T T E R , A . D . B R A B B A N A N D D . J . N I S B E T . 2003. Aims: To examine the effects of ionophores on Salmonella and Escherichia coli O157:H7 in pure and mixed ruminal fluid cultures. Methods and Results: Four Salmonella serotypes (Dublin, Derby, Typhimurium, and Enteriditis) and two strains of E. coli O157:H7 (ATCC 43895 and FDIU 6058) were cultured in the presence of varying concentrations of ionophores (monensin, lasalocid, laidlomycin propionate, and Bambermycin) in pure and mixed ruminal fluid cultures. Bacterial growth rates in pure culture were not affected (P >0 AE10) by ionophores at concentrations up to 10 times the approximate rumen ionophore concentration under normal feeding regimens. Likewise, ionophores had no effect (P >0 AE10) on Salmonella or E. coli CFU plated from 24-h ruminal fluid incubations. Ionophore treatment decreased (P
Patrick Butaye - One of the best experts on this subject based on the ideXlab platform.
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antimicrobial growth promoters used in animal feed effects of less well known antibiotics on gram positive bacteria
Clinical Microbiology Reviews, 2003Co-Authors: Patrick Butaye, L A Devriese, Freddy HaesebrouckAbstract:There are not many data available on antibiotics used solely in animals and almost exclusively for growth promotion. These products include Bambermycin, avilamycin, efrotomycin, and the ionophore antibiotics (monensin, salinomycin, narasin, and lasalocid). Information is also scarce for bacitracin used only marginally in human and veterinary medicine and for streptogramin antibiotics. The mechanisms of action of and resistance mechanisms against these antibiotics are described. Special emphasis is given to the prevalence of resistance among gram-positive bacteria isolated from animals and humans. Since no susceptibility breakpoints are available for most of the antibiotics discussed, an alternative approach to the interpretation of MICs is presented. Also, some pharmacokinetic data and information on the influence of these products on the intestinal flora are presented.
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Influence of different medium components on the in vitro activity of the growth-promoting antibiotic flavomycin against enterococci
The Journal of antimicrobial chemotherapy, 2000Co-Authors: Patrick Butaye, Luc Devriese, Freddy HaesebrouckAbstract:The growth-promoting antibiotic flavomycin (also called Bambermycin, flavophospholipol and moenomycin) has a complex spectrum of activity against enterococci, with some species being naturally resistant and others susceptible. In this study, proteins added to Mueller-Hinton II medium had a strong deleterious effect on the activity of flavomycin, glucose had no effect and starch decreased the activity of flavomycin. The fatty substances Tween 80 and tributyrin increased the activity of flavomycin for several enterococcal species. Slight differences in the composition of the susceptibility test medium affected the MIC results obtained, indicating that strict standardization of the test medium is necessary.
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In vitro susceptibility of Enterococcus faecium isolated from food to growth-promoting and therapeutic antibiotics
International journal of food microbiology, 2000Co-Authors: Patrick Butaye, Luc Devriese, K. Van Damme, L. R. Van Damme, Margo Baele, S Lauwers, Freddy HaesebrouckAbstract:A total of 76 E. faecium strains, isolated at retail level from raw poultry meat, cheese, raw pork, and preparations of cheese and raw pork, were tested for their susceptibility and resistance to growth-promoting antibacterials used in animals and antibiotics used therapeutically in humans. All strains were uniformly susceptible to the growth promoters Bambermycin and avilamycin. Resistance against bacitracin, virginiamycin and narasin was high among strains from poultry meat. With tylosin, a macrolide antibiotic used therapeutically and for growth promotion, resistance was mainly detected in strains originating from poultry meat, though also in some strains from pork and from pork and cheese preparations. The therapeutic antibiotic dalfopristin/quinupristin did not show full cross-resistance with the growth-promoting antibiotic virginiamycin. With dalfopristin/quinupristin two different levels of resistance were found. Only one E. faecium strain isolated from poultry was resistant to the glycopeptides avoparcin and vancomycin. Only one poultry meat strain was highly resistant to ampicillin. However, nearly all poultry meat strains showed decreased sensitivity. Only 3 out of 24 poultry strains were susceptible to minocycline, while all strains from other origins were susceptible to this tetracycline antibiotic. High-level streptomycin resistance was seen in strains of all origins, though infrequently. High-level gentamicin resistance was not found.
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Effects of Different Test Conditions on MICs of Food Animal Growth-Promoting Antibacterial Agents for Enterococci
Journal of clinical microbiology, 1998Co-Authors: Patrick Butaye, Luc Devriese, Freddy HaesebrouckAbstract:The influence of the addition of sheep blood to Mueller-Hinton II agar and the effects of aerobic incubation with or without CO2 and of anaerobic incubation were tested with bacitracin, tylosin, avoparcin, virginiamycin, avilamycin, narasin, and flavomycin on enterococci. The antibacterial activity of Bambermycin (Flavomycin) was strongly inhibited by the addition of blood, except with the species Enterococcus faecium, Enterococcus mundtii, Enterococcus hirae, Enterococcus casseliflavus, and Enterococcus gallinarum, which were not susceptible to this antibiotic on blood-free medium. With all other antimicrobials except avoparcin and tylosin, the presence of blood resulted in MIC increases of 1 to 3 log2 differences. Incubation in aerobic or anaerobic atmospheres enriched with CO2 lowered the susceptibility of enterococci to tylosin and increased their susceptibility to avilamycin, narasin, and avoparcin. This effect was most pronounced in tests on blood-free media. Results of susceptibility tests incubated under anaerobiosis and in a CO2-enriched atmosphere did not differ. For all enterococcal species, the preferred conditions for testing the susceptibility are Mueller-Hinton II medium supplemented with blood and incubation in a CO2-enriched atmosphere. However, when only E. faecium and Enterococcus faecalis are being tested, Mueller-Hinton II medium without blood incubated aerobically gives satisfactory results.
T R Callaway - One of the best experts on this subject based on the ideXlab platform.
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Effects of rotating antibiotic and ionophore feed additives on volatile fatty acid production, potential for methane production, and microbial populations of steers consuming a moderate-forage diet.
Journal of animal science, 2017Co-Authors: Whitney L Crossland, T R Callaway, Luis O Tedeschi, M. D. Miller, William B Smith, M D CraveyAbstract:Ionophores and antibiotics have been shown to decrease ruminal methanogenesis both in vitro and in vivo but have shown little evidence toward a sustainable means of mitigation. Feed additive rotation was proposed and investigated for methane, VFA, and microbial population response. In the present study, cannulated steers ( = 12) were fed a moderate-forage basal diet in a Calan gate facility for 13 wk. In addition to the basal diet, steers were randomly assigned to 1 of 6 treatments: 1) control, no additive; 2) Bambermycin, 20 mg Bambermycin/d; 3) monensin, 200 mg monensin/d; 4) the basal diet + weekly rotation of Bambermycin and monensin treatments (B7M); 5) the basal diet + rotation of Bambermycin and monensin treatments every 14 d (B14M); and 6) the basal diet + rotation of Bambermycin and monensin treatments every 21 d (B21M). Steers were blocked by weight in a randomized complete block design where the week was the repeated measure. Rumen fluid was collected weekly for analysis ( = 13), and results were normalized according to individual OM intake (OMI; kg/d). Potential activity of methane production was not significantly different among treatments ( > 0.05). However, treatment tended to affect the CH-to-propionate ratio ( = 0.0565), which was highest in the control and lowest in the monensin, B21M, and B14M treatments (0.42 vs. 0.36, 0.36, and 0.33, respectively). The CH:propionate ratio was lowest in wk 2 and 3 ( < 0.05) but the ratio in wk 4 to 12 was not different from the ratio in wk 0. Week also affected total VFA, with total VFA peaking at wk 3 and plummeting at wk 4 (4.02 vs. 2.86 m/kg OMI; < 0.05). A significant treatment × week interaction was observed for the acetate-to-propionate (A:P) ratio, where Bambermycin- and rotationally fed steers did not have a reduced A:P ratio compared with monensin-fed steers throughout the feeding period ( < 0.0001). Microbial analysis revealed significant shifts, but several predominant classes showed adaptation between 4 and 6 wk after additive initiation. There was no significant evidence to suggest that rotations of monensin and Bambermycin provided additional benefits to steers consuming a moderate-forage diet at the microbial/animal and environmental level versus those continuously fed.
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effects of the antibiotic ionophores monensin lasalocid laidlomycin propionate and Bambermycin on salmonella and e coli o157 h7 in vitro
Journal of Applied Microbiology, 2003Co-Authors: Thomas S. Edrington, T R Callaway, P D Varey, R O Elder, Elizabeth Kutter, Andrew D Brabban, Kenneth M. Bischoff, Y. S. Jung, Robin C Anderson, David J NisbetAbstract:T.S. E DRINGTON, T .R. C ALLAWAY, P .D. V AREY, Y .S. J UNG, K .M. B ISCHOFF, R .O. E LDER, R . C . A N D E R S O N , E . K U T T E R , A . D . B R A B B A N A N D D . J . N I S B E T . 2003. Aims: To examine the effects of ionophores on Salmonella and Escherichia coli O157:H7 in pure and mixed ruminal fluid cultures. Methods and Results: Four Salmonella serotypes (Dublin, Derby, Typhimurium, and Enteriditis) and two strains of E. coli O157:H7 (ATCC 43895 and FDIU 6058) were cultured in the presence of varying concentrations of ionophores (monensin, lasalocid, laidlomycin propionate, and Bambermycin) in pure and mixed ruminal fluid cultures. Bacterial growth rates in pure culture were not affected (P >0 AE10) by ionophores at concentrations up to 10 times the approximate rumen ionophore concentration under normal feeding regimens. Likewise, ionophores had no effect (P >0 AE10) on Salmonella or E. coli CFU plated from 24-h ruminal fluid incubations. Ionophore treatment decreased (P <0 AE01) the acetate : propionate ratio in ruminal fluid cultures as expected. Conclusions: Ionophores had no effect on the foodborne pathogens Salmonella and E. coli O157:H7 in vitro. Significance and Impact of the Study: The results suggest that ionophore feeding would have little or no effect on Salmonella or E. coli populations in the ruminant.
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effect of feeding the ionophores monensin and laidlomycin propionate and the antimicrobial Bambermycin to sheep experimentally infected with e coli o157 h7 and salmonella typhimurium
Journal of Animal Science, 2003Co-Authors: Thomas S. Edrington, T R Callaway, R O Elder, Kenneth M. Bischoff, Robin C Anderson, Kenneth J Genovese, David J NisbetAbstract:Escherichia coli O157:H7 and Salmo- nella are widely recognized as important agents of food- borne disease with worldwide distribution. The use of ionophores in feeding growing ruminants is widespread in the United States and has attracted recent interest due to the apparent temporal relationship between ini- tial ionophore use and the increase in human E. coli O157:H7 cases. Two experiments were conducted to evaluate the effects of short-term feeding of ionophores on fecal shedding, intestinal concentrations, and anti- microbial susceptibility of E. coli O157:H7 and S. typhi- murium in growing lambs. Sixteen lambs were used in each experiment, four lambs per treatment group: monensin, laidlomycin propionate, Bambermycin, and a control treatment. Lambs were fed a grain and hay (50:50) diet with their respective ionophore for 12 d before experimental inoculation with E. coli O157:H7
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Effects of the antibiotic ionophores monensin, lasalocid, laidlomycin propionate and Bambermycin on Salmonella and E. coli O157:H7 in vitro*†
Journal of applied microbiology, 2003Co-Authors: Thomas S. Edrington, T R Callaway, P D Varey, R O Elder, Elizabeth Kutter, Andrew D Brabban, Kenneth M. Bischoff, Y. S. Jung, Robin C Anderson, David J NisbetAbstract:T.S. E DRINGTON, T .R. C ALLAWAY, P .D. V AREY, Y .S. J UNG, K .M. B ISCHOFF, R .O. E LDER, R . C . A N D E R S O N , E . K U T T E R , A . D . B R A B B A N A N D D . J . N I S B E T . 2003. Aims: To examine the effects of ionophores on Salmonella and Escherichia coli O157:H7 in pure and mixed ruminal fluid cultures. Methods and Results: Four Salmonella serotypes (Dublin, Derby, Typhimurium, and Enteriditis) and two strains of E. coli O157:H7 (ATCC 43895 and FDIU 6058) were cultured in the presence of varying concentrations of ionophores (monensin, lasalocid, laidlomycin propionate, and Bambermycin) in pure and mixed ruminal fluid cultures. Bacterial growth rates in pure culture were not affected (P >0 AE10) by ionophores at concentrations up to 10 times the approximate rumen ionophore concentration under normal feeding regimens. Likewise, ionophores had no effect (P >0 AE10) on Salmonella or E. coli CFU plated from 24-h ruminal fluid incubations. Ionophore treatment decreased (P
