The Experts below are selected from a list of 585 Experts worldwide ranked by ideXlab platform
Larissa V. Rodriguez - One of the best experts on this subject based on the ideXlab platform.
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effects of water avoidance stress on peripheral and central responses during bladder filling in the rat a multidisciplinary approach to the study of urologic chronic pelvic pain syndrome mapp research network study
PLOS ONE, 2017Co-Authors: Zhuo Wang, Harriet H. Chang, Rong Zhang, Daniel P. Holschneider, Larissa V. RodriguezAbstract:: Stress plays a role in the exacerbation and possibly the development of functional lower urinary tract disorders. Chronic water avoidance stress (WAS) in rodents is a model with high construct and face validity to bladder hypersensitive syndromes, such as interstitial cystitis/bladder pain syndrome (IC/BPS), characterized by urinary frequency and bladder hyperalgesia and heightened stress responsiveness. Given the overlap of the brain circuits involved in stress, anxiety, and micturition, we evaluated the effects chronic stress has on bladder function, as well as its effects on regional brain activation during bladder filling. Female Wistar-Kyoto rats were exposed to WAS (10 days) or sham paradigms. One day thereafter, cystometrograms were obtained during titrated bladder dilation, with visceromotor responses (VMR) recorded simultaneously. Cerebral perfusion was assessed during passive bladder distension (20-cmH2O) following intravenous administration of [14C]-iodoantipyrine. Regional cerebral blood flow was quantified by autoradiography and analyzed in 3-dimensionally reconstructed brains with statistical parametric mapping. WAS animals compared to controls demonstrated a decreased pressure threshold and visceromotor threshold triggering the voiding phase. At 20-cmH2O, VMR was significantly greater in WAS animals compared to controls. WAS animals showed greater activation in cortical regions of the central micturition circuit, including the posterior cingulate, anterior retrosplenial, somatosensory, posterior insula, orbital, and anterior secondary ("supplementary") motor cortices, as well as in the thalamus, anterior hypothalamus, parabrachial and Barrington nuclei, and striatum. Seed analysis showed increased functional connectivity of WAS compared to control animals of the posterior cingulate cortex to the pontine parabrachial Nucleus; of the Barrington Nucleus to the anterior dorsal midline and ventrobasilar thalamus and somatosensory and retrosplenial cortices; and of the posterior insula to anterior secondary motor cortex. Our findings show a visceral hypersensitivity during bladder filling in WAS animals, as well as increased engagement of portions of the micturition circuit responsive to urgency, viscerosensory perception and its relay to motor regions coordinating imminent bladder contraction. Results are consistent with recent findings in patients with interstitial cystitis, suggesting that WAS may serve as an animal model to elucidate the mechanisms leading to viscerosensitive brain phenotypes in humans with IC/BPS.
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Seed correlation of functional activity across the whole brain during bladder filling.
2017Co-Authors: Zhuo Wang, Harriet H. Chang, Yunliang Gao, Rong Zhang, Yumei Guo, Daniel P. Holschneider, Larissa V. RodriguezAbstract:WAS compared to control rats show greater functional connectivity of (row 1) the posterior cingulate to the parabrachial/Barrington Nucleus complex, (row 2) the posterior insula to anterior secondary motor cortex (M2), and (row 3) the Barrington Nucleus to the anterior, dorsal midline thalamus (mediodorsal, MD; paraventricular, PVN), as well as the ventrobasilar thalamic complex, primary and secondary somatosensory cortex (S1, S2) and retrosplenial cortex.
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Detailed view of functional brain activation during bladder filling of WAS rats compared to controls at the level of the pontine micturition center (9.1 and 9.4 mm posterior to bregma).
2017Co-Authors: Zhuo Wang, Harriet H. Chang, Yunliang Gao, Rong Zhang, Yumei Guo, Daniel P. Holschneider, Larissa V. RodriguezAbstract:Significant differences in regional CBF during bladder filling of WAS rats compared to controls (red indicates increased rCBF, blue indicates decreased rCBF). Also shown are the region-of-interest definition (green) for the Barrington Nucleus (BN) and parabrachial Nucleus (PBN) used in our analyses, as well as the landmark for the dorsal tegmentum (DTg).
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Summary of the significant changes in regional cerebral blood flow (rCBF) of WAS vs. control rats during passive bladder filling as related to a simplified model of the micturition circuit.
2017Co-Authors: Zhuo Wang, Harriet H. Chang, Yunliang Gao, Rong Zhang, Yumei Guo, Daniel P. Holschneider, Larissa V. RodriguezAbstract:Data are summarized from the SPM analysis shown in Fig 3, with red indicating significant increases in rCBF and blue indicating significant decreases in rCBF. Abbreviations: Cx (cortex), HPC (hippocampus), lPFC (lateral prefrontal cortex), mPFC (medial prefrontal cortex), PAG (periaqueductal gray), PMC (pontine micturition center, parabrachial/Barrington Nucleus complex), rCBF (regional cerebral blood flow). Adapted from Griffiths [36] and DeGroat and Yoshimura [37].
Zhuo Wang - One of the best experts on this subject based on the ideXlab platform.
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effects of water avoidance stress on peripheral and central responses during bladder filling in the rat a multidisciplinary approach to the study of urologic chronic pelvic pain syndrome mapp research network study
PLOS ONE, 2017Co-Authors: Zhuo Wang, Harriet H. Chang, Rong Zhang, Daniel P. Holschneider, Larissa V. RodriguezAbstract:: Stress plays a role in the exacerbation and possibly the development of functional lower urinary tract disorders. Chronic water avoidance stress (WAS) in rodents is a model with high construct and face validity to bladder hypersensitive syndromes, such as interstitial cystitis/bladder pain syndrome (IC/BPS), characterized by urinary frequency and bladder hyperalgesia and heightened stress responsiveness. Given the overlap of the brain circuits involved in stress, anxiety, and micturition, we evaluated the effects chronic stress has on bladder function, as well as its effects on regional brain activation during bladder filling. Female Wistar-Kyoto rats were exposed to WAS (10 days) or sham paradigms. One day thereafter, cystometrograms were obtained during titrated bladder dilation, with visceromotor responses (VMR) recorded simultaneously. Cerebral perfusion was assessed during passive bladder distension (20-cmH2O) following intravenous administration of [14C]-iodoantipyrine. Regional cerebral blood flow was quantified by autoradiography and analyzed in 3-dimensionally reconstructed brains with statistical parametric mapping. WAS animals compared to controls demonstrated a decreased pressure threshold and visceromotor threshold triggering the voiding phase. At 20-cmH2O, VMR was significantly greater in WAS animals compared to controls. WAS animals showed greater activation in cortical regions of the central micturition circuit, including the posterior cingulate, anterior retrosplenial, somatosensory, posterior insula, orbital, and anterior secondary ("supplementary") motor cortices, as well as in the thalamus, anterior hypothalamus, parabrachial and Barrington nuclei, and striatum. Seed analysis showed increased functional connectivity of WAS compared to control animals of the posterior cingulate cortex to the pontine parabrachial Nucleus; of the Barrington Nucleus to the anterior dorsal midline and ventrobasilar thalamus and somatosensory and retrosplenial cortices; and of the posterior insula to anterior secondary motor cortex. Our findings show a visceral hypersensitivity during bladder filling in WAS animals, as well as increased engagement of portions of the micturition circuit responsive to urgency, viscerosensory perception and its relay to motor regions coordinating imminent bladder contraction. Results are consistent with recent findings in patients with interstitial cystitis, suggesting that WAS may serve as an animal model to elucidate the mechanisms leading to viscerosensitive brain phenotypes in humans with IC/BPS.
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Seed correlation of functional activity across the whole brain during bladder filling.
2017Co-Authors: Zhuo Wang, Harriet H. Chang, Yunliang Gao, Rong Zhang, Yumei Guo, Daniel P. Holschneider, Larissa V. RodriguezAbstract:WAS compared to control rats show greater functional connectivity of (row 1) the posterior cingulate to the parabrachial/Barrington Nucleus complex, (row 2) the posterior insula to anterior secondary motor cortex (M2), and (row 3) the Barrington Nucleus to the anterior, dorsal midline thalamus (mediodorsal, MD; paraventricular, PVN), as well as the ventrobasilar thalamic complex, primary and secondary somatosensory cortex (S1, S2) and retrosplenial cortex.
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Detailed view of functional brain activation during bladder filling of WAS rats compared to controls at the level of the pontine micturition center (9.1 and 9.4 mm posterior to bregma).
2017Co-Authors: Zhuo Wang, Harriet H. Chang, Yunliang Gao, Rong Zhang, Yumei Guo, Daniel P. Holschneider, Larissa V. RodriguezAbstract:Significant differences in regional CBF during bladder filling of WAS rats compared to controls (red indicates increased rCBF, blue indicates decreased rCBF). Also shown are the region-of-interest definition (green) for the Barrington Nucleus (BN) and parabrachial Nucleus (PBN) used in our analyses, as well as the landmark for the dorsal tegmentum (DTg).
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Summary of the significant changes in regional cerebral blood flow (rCBF) of WAS vs. control rats during passive bladder filling as related to a simplified model of the micturition circuit.
2017Co-Authors: Zhuo Wang, Harriet H. Chang, Yunliang Gao, Rong Zhang, Yumei Guo, Daniel P. Holschneider, Larissa V. RodriguezAbstract:Data are summarized from the SPM analysis shown in Fig 3, with red indicating significant increases in rCBF and blue indicating significant decreases in rCBF. Abbreviations: Cx (cortex), HPC (hippocampus), lPFC (lateral prefrontal cortex), mPFC (medial prefrontal cortex), PAG (periaqueductal gray), PMC (pontine micturition center, parabrachial/Barrington Nucleus complex), rCBF (regional cerebral blood flow). Adapted from Griffiths [36] and DeGroat and Yoshimura [37].
Maria Trinidad Herrero - One of the best experts on this subject based on the ideXlab platform.
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Critical evaluation of the anatomical location of the Barrington Nucleus: Relevance for deep brain stimulation surgery of pedunculopontine tegmental Nucleus
Neuroscience, 2013Co-Authors: Lisette Blanco, José Enrique Yuste, María Angeles Carrillo-de Sauvage, A. Gomez, Emiliano Fernández-villalba, Itciar Avilés-olmos, Patricia Limousin, Ludvic Zrinzo, Maria Trinidad HerreroAbstract:Deep brain stimulation (DBS) has become the standard surgical procedure for advanced Parkinson's disease (PD). Recently, the pedunculopontine tegmental Nucleus (PPN) has emerged as a potential target for DBS in patients whose quality of life is compromised by freezing of gait and falls. To date, only a few groups have published their long-term clinical experience with PPN stimulation. Bearing in mind that the Barrington (Bar) Nucleus and some adjacent nuclei (also known as the micturition centre) are close to the PPN and may be affected by DBS, the aim of the present study was to review the anatomical location of this structure in human and other species. To this end, the Bar Nucleus area was analysed in mouse, monkey and human tissues, paying particular attention to the anatomical position in humans, where it has been largely overlooked. Results confirm that anatomical location renders the Bar Nucleus susceptible to influence by the PPN DBS lead or to diffusion of electrical current. This may have an undesirable impact on the quality of life of patients.
Isabel Rocha - One of the best experts on this subject based on the ideXlab platform.
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effect on urinary bladder function and arterial blood pressure of the activation of putative purine receptors in brainstem areas
Autonomic Neuroscience: Basic and Clinical, 2001Co-Authors: Isabel Rocha, Geoffrey Burnstock, Michael K SpyerAbstract:Abstract The effect on bladder function and arterial blood pressure of adenosine-5′-triphosphate (ATP) and its synthetic analogue, α,β-methylene ATP (α,β-meATP) applied by microinjection to brainstem areas was assessed in the anaesthetised, paralysed and artificially ventilated female rat. Recordings of bladder pressure, changes in the pelvic nerve activity, arterial blood pressure and heart rate were evaluated. The purinergic drugs were microinjected into two brainstem areas – the periaqueductal grey matter (PAG) and the area of the Barrington Nucleus/locus coeruleus (LC) – only after electrical stimulation (50 Hz, 1 ms, 30–50 μA; n (PAG) =17; n (LC) =18) and the microinjection of glutamate (2 mM, pH 7.4±0.1; n (PAG) =16; n (LC) =16) had shown increases of bladder pressure and/or rate of bladder contractions and/or pelvic nerve activity at specific sites. Electrical and glutamate activation of PAG evoked an increase of arterial blood pressure. Microinjections of ATP (20 mM, pH 7.4±0.1; n (PAG) =11; n (LC) =11) and α,β-meATP (2 mM, pH 7.4±0.1; n (PAG) =10; n (LC) =9) both evoked consistent increases of bladder pressure and/or pelvic nerve activity. Stimulation with ATP elicited a biphasic change of arterial blood pressure characterised by an increase followed by a decrease which was accompanied by a rise of heart rate. Microinjection of α,β-meATP into PAG did not elicit a consistent response: a decrease of arterial blood pressure was evoked in five rats, while in two other rats an increase occurred. Electrical stimulation and glutamate activation of Barrington’s Nucleus/LC evoked an increase of arterial blood pressure, but a decrease was observed after microinjection of both ATP and α,β-meATP. At some sites ( n =8) the effect of α,β-meATP after a pre-injection at the same site of the P2 purino receptor antagonist, suramin (20 mM, pH 7.4±0.1) was smaller than the control. At three sites within PAG and two within LC located more medially to sites where an excitatory response had been observed, electrical stimulation evoked a small decrease or no change in bladder pressure. Following the stimulus, a rise in bladder pressure was preceded by an increase of pelvic nerve activity. A similar effect of glutamate was observed in one case. These data suggest that activation of P2 purine receptors in both PAG and Barrington’s Nucleus/LC is implicated in the neuronal mechanisms that generate patterns of activity in the parasympathetic innervation of the bladder and that purines also act at this level to modify sympathetic outflow to the cardiovascular system.
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Effect on urinary bladder function and arterial blood pressure of the activation of putative purine receptors in brainstem areas.
Autonomic neuroscience : basic & clinical, 2001Co-Authors: Isabel Rocha, Geoffrey Burnstock, K M SpyerAbstract:The effect on bladder function and arterial blood pressure of adenosine-5'-triphosphate (ATP) and its synthetic analogue, alpha,beta-methylene ATP (alpha,beta-meATP) applied by microinjection to brainstem areas was assessed in the anaesthetised, paralysed and artificially ventilated female rat. Recordings of bladder pressure, changes in the pelvic nerve activity, arterial blood pressure and heart rate were evaluated. The purinergic drugs were microinjected into two brainstem areas the periaqueductal grey matter (PAG) and the area of the Barrington Nucleus/locus coeruleus (LC) - only after electrical stimulation (50 Hz, 1 ms, 30-50 microA; n(PAG) = 17; n(LC) = 18) and the microinjection of glutamate (2 mM, pH 7.4+/-0.1; n(PAG) = 16; n(LC) = 16) had shown increases of bladder pressure and/or rate of bladder contractions and/or pelvic nerve activity at specific sites. Electrical and glutamate activation of PAG evoked an increase of arterial blood pressure. Microinjections of ATP (20 mM, pH 7.4+/-0.1; n(PAG) = 11; n(LC) = 11) and alpha,beta-meATP (2 mM, pH 7.4+/-0.1; n(PAG) = 10; n(LC) = 9) both evoked consistent increases of bladder pressure and/or pelvic nerve activity. Stimulation with ATP elicited a biphasic change of arterial blood pressure characterised by an increase followed by a decrease which was accompanied by a rise of heart rate. Microinjection of alpha,beta-meATP into PAG did not elicit a consistent response: a decrease of arterial blood pressure was evoked in five rats, while in two other rats an increase occurred. Electrical stimulation and glutamate activation of Barrington's Nucleus/LC evoked an increase of arterial blood pressure, but a decrease was observed after microinjection of both ATP and alpha,beta-meATP. At some sites (n = 8) the effect of alpha,beta-meATP after a pre-injection at the same site of the P2 purino receptor antagonist, suramin (20 mM, pH 7.4+/-0.1) was smaller than the control. At three sites within PAG and two within LC located more medially to sites where an excitatory response had been observed, electrical stimulation evoked a small decrease or no change in bladder pressure. Following the stimulus, a rise in bladder pressure was preceded by an increase of pelvic nerve activity. A similar effect of glutamate was observed in one case. These data suggest that activation of P2 purine receptors in both PAG and Barrington's Nucleus/LC is implicated in the neuronal mechanisms that generate patterns of activity in the parasympathetic innervation of the bladder and that purines also act at this level to modify sympathetic outflow to the cardiovascular system.
Harriet H. Chang - One of the best experts on this subject based on the ideXlab platform.
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effects of water avoidance stress on peripheral and central responses during bladder filling in the rat a multidisciplinary approach to the study of urologic chronic pelvic pain syndrome mapp research network study
PLOS ONE, 2017Co-Authors: Zhuo Wang, Harriet H. Chang, Rong Zhang, Daniel P. Holschneider, Larissa V. RodriguezAbstract:: Stress plays a role in the exacerbation and possibly the development of functional lower urinary tract disorders. Chronic water avoidance stress (WAS) in rodents is a model with high construct and face validity to bladder hypersensitive syndromes, such as interstitial cystitis/bladder pain syndrome (IC/BPS), characterized by urinary frequency and bladder hyperalgesia and heightened stress responsiveness. Given the overlap of the brain circuits involved in stress, anxiety, and micturition, we evaluated the effects chronic stress has on bladder function, as well as its effects on regional brain activation during bladder filling. Female Wistar-Kyoto rats were exposed to WAS (10 days) or sham paradigms. One day thereafter, cystometrograms were obtained during titrated bladder dilation, with visceromotor responses (VMR) recorded simultaneously. Cerebral perfusion was assessed during passive bladder distension (20-cmH2O) following intravenous administration of [14C]-iodoantipyrine. Regional cerebral blood flow was quantified by autoradiography and analyzed in 3-dimensionally reconstructed brains with statistical parametric mapping. WAS animals compared to controls demonstrated a decreased pressure threshold and visceromotor threshold triggering the voiding phase. At 20-cmH2O, VMR was significantly greater in WAS animals compared to controls. WAS animals showed greater activation in cortical regions of the central micturition circuit, including the posterior cingulate, anterior retrosplenial, somatosensory, posterior insula, orbital, and anterior secondary ("supplementary") motor cortices, as well as in the thalamus, anterior hypothalamus, parabrachial and Barrington nuclei, and striatum. Seed analysis showed increased functional connectivity of WAS compared to control animals of the posterior cingulate cortex to the pontine parabrachial Nucleus; of the Barrington Nucleus to the anterior dorsal midline and ventrobasilar thalamus and somatosensory and retrosplenial cortices; and of the posterior insula to anterior secondary motor cortex. Our findings show a visceral hypersensitivity during bladder filling in WAS animals, as well as increased engagement of portions of the micturition circuit responsive to urgency, viscerosensory perception and its relay to motor regions coordinating imminent bladder contraction. Results are consistent with recent findings in patients with interstitial cystitis, suggesting that WAS may serve as an animal model to elucidate the mechanisms leading to viscerosensitive brain phenotypes in humans with IC/BPS.
-
Seed correlation of functional activity across the whole brain during bladder filling.
2017Co-Authors: Zhuo Wang, Harriet H. Chang, Yunliang Gao, Rong Zhang, Yumei Guo, Daniel P. Holschneider, Larissa V. RodriguezAbstract:WAS compared to control rats show greater functional connectivity of (row 1) the posterior cingulate to the parabrachial/Barrington Nucleus complex, (row 2) the posterior insula to anterior secondary motor cortex (M2), and (row 3) the Barrington Nucleus to the anterior, dorsal midline thalamus (mediodorsal, MD; paraventricular, PVN), as well as the ventrobasilar thalamic complex, primary and secondary somatosensory cortex (S1, S2) and retrosplenial cortex.
-
Detailed view of functional brain activation during bladder filling of WAS rats compared to controls at the level of the pontine micturition center (9.1 and 9.4 mm posterior to bregma).
2017Co-Authors: Zhuo Wang, Harriet H. Chang, Yunliang Gao, Rong Zhang, Yumei Guo, Daniel P. Holschneider, Larissa V. RodriguezAbstract:Significant differences in regional CBF during bladder filling of WAS rats compared to controls (red indicates increased rCBF, blue indicates decreased rCBF). Also shown are the region-of-interest definition (green) for the Barrington Nucleus (BN) and parabrachial Nucleus (PBN) used in our analyses, as well as the landmark for the dorsal tegmentum (DTg).
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Summary of the significant changes in regional cerebral blood flow (rCBF) of WAS vs. control rats during passive bladder filling as related to a simplified model of the micturition circuit.
2017Co-Authors: Zhuo Wang, Harriet H. Chang, Yunliang Gao, Rong Zhang, Yumei Guo, Daniel P. Holschneider, Larissa V. RodriguezAbstract:Data are summarized from the SPM analysis shown in Fig 3, with red indicating significant increases in rCBF and blue indicating significant decreases in rCBF. Abbreviations: Cx (cortex), HPC (hippocampus), lPFC (lateral prefrontal cortex), mPFC (medial prefrontal cortex), PAG (periaqueductal gray), PMC (pontine micturition center, parabrachial/Barrington Nucleus complex), rCBF (regional cerebral blood flow). Adapted from Griffiths [36] and DeGroat and Yoshimura [37].
