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G. Riba - One of the best experts on this subject based on the ideXlab platform.

M Rougier - One of the best experts on this subject based on the ideXlab platform.

Nemat O. Keyhani - One of the best experts on this subject based on the ideXlab platform.

  • molecular genetics of Beauveria bassiana infection of insects
    Advances in Genetics, 2016
    Co-Authors: Almudena Ortizurquiza, Nemat O. Keyhani
    Abstract:

    Research on the insect pathogenic filamentous fungus, Beauveria bassiana has witnessed significant growth in recent years from mainly physiological studies related to its insect biological control potential, to addressing fundamental questions regarding the underlying molecular mechanisms of fungal development and virulence. This has been in part due to a confluence of robust genetic tools and genomic resources for the fungus, and recognition of expanded ecological interactions with which the fungus engages. Beauveria bassiana is a broad host range insect pathogen that has the ability to form intimate symbiotic relationships with plants. Indeed, there is an increasing realization that the latter may be the predominant environmental interaction in which the fungus participates, and that insect parasitism may be an opportunist lifestyle evolved due to the carbon- and nitrogen-rich resources present in insect bodies. Here, we will review progress on the molecular genetics of B. bassiana, which has largely been directed toward identifying genetic pathways involved in stress response and virulence assumed to have practical applications in improving the insect control potential of the fungus. Important strides have also been made in understanding aspects of B. bassiana development. Finally, although increasingly apparent in a number of studies, there is a need for progressing beyond phenotypic mutant characterization to sufficiently investigate the molecular mechanisms underlying B. bassiana's unique and diverse lifestyles as saprophyte, insect pathogen, and plant mutualist.

  • Molecular and immunological characterization of allergens from the entomopathogenic fungus Beauveria bassiana
    Clinical and Molecular Allergy, 2006
    Co-Authors: Greg S Westwood, Shih-wen Huang, Nemat O. Keyhani
    Abstract:

    Background Entomopathogenic fungi such as Beauveria bassiana are considered promising biological control agents for a variety of arthropod pests. Beauveria species, however, have the potential to elicit allergenic reactions in humans, although no specific allergens have been characterized to date.

  • Allergens of the entomopathogenic fungus Beauveria bassiana
    Clinical and Molecular Allergy, 2005
    Co-Authors: Greg S Westwood, Shih-wen Huang, Nemat O. Keyhani
    Abstract:

    Background Beauveria bassiana is an important entomopathogenic fungus currently under development as a bio-control agent for a variety of insect pests. Although reported to be non-toxic to vertebrates, the potential allergenicity of Beauveria species has not been widely studied. Methods IgE-reactivity studies were performed using sera from patients displaying mould hypersensitivity by immunoblot and immunoblot inhibition. Skin reactivity to B. bassiana extracts was measured using intradermal skin testing. Results Immunoblots of fungal extracts with pooled as well as individual sera showed a distribution of IgE reactive proteins present in B. bassiana crude extracts. Proteinase K digestion of extracts resulted in loss of IgE reactive epitopes, whereas EndoH and PNGaseF (glycosidase) treatments resulted in minor changes in IgE reactive banding patterns as determined by Western blots. Immunoblot inhibitions experiments showed complete loss of IgE-binding using self protein, and partial inhibition using extracts from common allergenic fungi including; Alternaria alternata , Aspergillus fumigatus , Cladosporium herbarum , Candida albicans , Epicoccum purpurascens , and Penicillium notatum . Several proteins including a strongly reactive band with an approximate molecular mass of 35 kDa was uninhibited by any of the tested extracts, and may represent B. bassiana specific allergens. Intradermal skin testing confirmed the in vitro results, demonstrating allergenic reactions in a number of individuals, including those who have had occupational exposure to B. bassiana . Conclusions Beauveria bassiana possesses numerous IgE reactive proteins, some of which are cross-reactive among allergens from other fungi. A strongly reactive potential B. bassiana specific allergen (35 kDa) was identified. Intradermal skin testing confirmed the allergenic potential of B. bassiana .

  • Allergens of the entomopathogenic fungus Beauveria bassiana.
    Clinical and Molecular Allergy, 2005
    Co-Authors: Greg S Westwood, Shih-wen Huang, Nemat O. Keyhani
    Abstract:

    Background Beauveria bassiana is an important entomopathogenic fungus currently under development as a bio-control agent for a variety of insect pests. Although reported to be non-toxic to vertebrates, the potential allergenicity of Beauveria species has not been widely studied.

Malalanirina S Rakotonirainy - One of the best experts on this subject based on the ideXlab platform.

Song Jia - One of the best experts on this subject based on the ideXlab platform.

  • The HFW-05 Strains of Beauveria bassiana in Persistent Study on Vegetables
    2015
    Co-Authors: Song Jia
    Abstract:

    This paper studied using conventional foliar spray HFW-05 strain vegetables on persistent condition.Before applying bassiana fungus,bacteria and actinomycetes background was investigated. It used 2. 5 × 107 spores / m L concentration for three consecutive times,each time interval 5 d. The results showed Beauveria bassiana on different host plants into colonies wavy within two months. On the surface of different crop leaves,Beauveria bassiana eventually reached 106 cfu / g in fresh leaves eventually persistent stock level. The final persistent quantity of Beauveria bassiana in mesophyll of fresh leaf level reached 103 cfu / g. Meanwhile,the environmental release of Beauveria bacteria,actinomyces quantitative impact was not obvious,does not destroy the original ecological balance.

  • The Preliminary Study of Beauveria bassiana Survived in Soil
    2015
    Co-Authors: Song Jia
    Abstract:

    In order to research the Beauveria bassiana HFW-05 strain in soil in persistent storage,the soil mix bacteria method had carried on the preliminary exploration. Initial bacterial biomass was 1. 0 × 107 spore / m L concentrations. Continuous observation 60 d,the results showed that Beauveria bassiana in soil persistent within two months,a number of colonies showed a downward trend involatility,slower rate of decline,in the release of 30-60 d,Beauveria bassiana in a stable state of fluctuation,CFU continued in the 106 cfu / g. At the same time,the number of soil microorganism population had little effect. There was no significant difference before and after the release,the virulence of Beauveria bassiana against Plutella xylostella,didn't destroy the original ecological balance.