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Laura M. Boykin - One of the best experts on this subject based on the ideXlab platform.

  • Updated mtCOI reference dataset for the Bemisia tabaci species complex.
    F1000Research, 2017
    Co-Authors: Laura M. Boykin, Anders Savill, Paul J. De Barro
    Abstract:

    Members of the whitefly Bemisia tabaci species complex cause millions of dollars of damage globally and are considered one of the world’s most invasive species. They are capable of causing extensive damage to major vegetable, grain legume and fiber crops. All member of the species complex are morphologically identical therefore, data from the partial mitochondrial cytochrome oxidase subunit I (mtCOI) gene sequence has been used to identify the various species. The current reference dataset that is widely used is found on the CSIRO data portal. However, the reference set stored on the CSIRO data does not include newly added sequences (2013-2017), therefore an updated reference dataset is needed.  All mtCOI data for the Bemisia tabaci species complex were downloaded on 22 May 2017 from GenBank and after quality checking, a dataset of 1,071 unique sequences and 696 base pairs was generated (https://doi.org/10.6084/m9.figshare.5437420.v1).

  • Bemisia tabaci nomenclature lessons learned
    Pest Management Science, 2014
    Co-Authors: Laura M. Boykin
    Abstract:

    BACKGROUND The nomenclature used within the whitefly research community for different putative species within Bemisia tabaci (sensu Russell) remains highly variable and confused. This was evident by the many different naming schemes researchers were using in their presentations at the 1st International Whitefly Symposium in Kolymbari, Crete, Greece (20–24 May 2013). I wanted to try to document how we, as a community, have arrived at such a state of confused nomenclature. This also included an investigation of the nomenclature used in the literature (from 2002 to 2012) by means of two online search tools (Web of Science and Scirus). RESULTS Nomenclatural data were collected at the 1st International Whitefly Symposium, based on oral presentations and posters. There were 17 different names used for the MED species and 12 different names used for the MEAM1 species of the B. tabaci species complex. Investigation of the literature revealed limited uptake of the intermediate names. CONCLUSION The intermediate names for the various species in the B. tabaci species complex – MED, MEAM1, New World, etc. – are not being used by the wider whitefly community. To move forwarrd as a community, we must work towards a formal revision of the B. tabaci complex. © 2013 Society of Chemical Industry

  • Bemisia tabaci a statement of species status
    Annual Review of Entomology, 2011
    Co-Authors: Paul J. De Barro, Laura M. Boykin, Adam B Dinsdale
    Abstract:

    Bemisia tabaci has long been considered a complex species. It rose to global prominence in the 1980s owing to the global invasion by the commonly named B biotype. Since then, the concomitant eruption of a group of plant viruses known as begomoviruses has created considerable management problems in many countries. However, an enduring set of questions remains: Is B. tabaci a complex species or a species complex, what are Bemisia biotypes, and how did all the genetic variability arise? This review considers these issues and concludes that there is now sufficient evidence to state that B. tabaci is not made up of biotypes and that the use of biotype in this context is erroneous and misleading. Instead, B. tabaci is a complex of 11 well-defined high-level groups containing at least 24 morphologically indistinguishable species.

  • improved dna barcoding method for Bemisia tabaci and related aleyrodidae development of universal and Bemisia tabaci biotype specific mitochondrial cytochrome c oxidase i polymerase chain reaction primers
    Journal of Economic Entomology, 2009
    Co-Authors: Robert G. Shatters, Laura M. Boykin, Charles A Powell, He Liansheng, Cindy L. Mckenzie
    Abstract:

    ABSTRACT Whiteflies, heteropterans in the family Aleyrodidae, are globally distributed and severe agricultural pests. The mitochondrial cytochrome c oxidase I (mtCOI) sequence has been used extensively in whitefly phylogenetic comparisons and in biotype identification of the agriculturally important Bemisia tabaci (Gennadius) whitefly. Because of the economic importance of several whitefly genera, and the invasive nature of the B and the Q biotypes of Bemisia tabaci, mtCOI sequence data are continually generated from sampled populations worldwide. Routine phylogenetic comparisons and biotype identification is done through amplification and sequencing of an ≈800-bp mtCOI DNA fragment. Despite its routine use, published primers for amplification of this region are often inefficient for some B. tabaci biotypes and especially across whitefly species. Through new sequence generation and comparison to available whitefly mtCOI sequence data, a set of polymerase chain reaction (PCR) amplification primers (Btab-Un...

Paul J. De Barro - One of the best experts on this subject based on the ideXlab platform.

  • Updated mtCOI reference dataset for the Bemisia tabaci species complex.
    F1000Research, 2017
    Co-Authors: Laura M. Boykin, Anders Savill, Paul J. De Barro
    Abstract:

    Members of the whitefly Bemisia tabaci species complex cause millions of dollars of damage globally and are considered one of the world’s most invasive species. They are capable of causing extensive damage to major vegetable, grain legume and fiber crops. All member of the species complex are morphologically identical therefore, data from the partial mitochondrial cytochrome oxidase subunit I (mtCOI) gene sequence has been used to identify the various species. The current reference dataset that is widely used is found on the CSIRO data portal. However, the reference set stored on the CSIRO data does not include newly added sequences (2013-2017), therefore an updated reference dataset is needed.  All mtCOI data for the Bemisia tabaci species complex were downloaded on 22 May 2017 from GenBank and after quality checking, a dataset of 1,071 unique sequences and 696 base pairs was generated (https://doi.org/10.6084/m9.figshare.5437420.v1).

  • Bemisia tabaci a statement of species status
    Annual Review of Entomology, 2011
    Co-Authors: Paul J. De Barro, Laura M. Boykin, Adam B Dinsdale
    Abstract:

    Bemisia tabaci has long been considered a complex species. It rose to global prominence in the 1980s owing to the global invasion by the commonly named B biotype. Since then, the concomitant eruption of a group of plant viruses known as begomoviruses has created considerable management problems in many countries. However, an enduring set of questions remains: Is B. tabaci a complex species or a species complex, what are Bemisia biotypes, and how did all the genetic variability arise? This review considers these issues and concludes that there is now sufficient evidence to state that B. tabaci is not made up of biotypes and that the use of biotype in this context is erroneous and misleading. Instead, B. tabaci is a complex of 11 well-defined high-level groups containing at least 24 morphologically indistinguishable species.

Anastasia Tsagkarakou - One of the best experts on this subject based on the ideXlab platform.

  • Insecticide resistance in Bemisia tabaci (Homoptera: Aleyrodidae) populations from Crete.
    Pest Management Science, 2020
    Co-Authors: Emmanouil Roditakis, N. E. Roditakis, Anastasia Tsagkarakou
    Abstract:

    The resistance levels to alpha-cypermethrin, bifenthrin, pirimiphos-methyl, endosulfan and imidacloprid were determined in Bemisia tabaci (Gennadius) from Crete. Five B tabaci populations collected from greenhouse and outdoor crops were bioassayed and compared with a reference susceptible strain. Bemisia tabaci collected in a floriculture greenhouse exhibited the highest resistance against all insecticides: at LC50, resistance factors were 23-fold for bifenthrin, 80-fold for alpha-cypermethrin, 18-fold for pirimiphos-methyl, 58-fold for endosulfan and 730-fold for imidacloprid. A population collected on outdoor melons was more susceptible than the reference strain against all insecticides tested, suggesting the occurrence of local highly susceptible B tabaci populations in 'refugia'. In pairwise comparisons of resistance levels, correlation was observed between the LC50 values of the pyrethroid insecticides bifenthrin and alpha-cypermethrin.

  • insecticide resistance in Bemisia tabaci homoptera aleyrodidae populations from crete
    Pest Management Science, 2005
    Co-Authors: Emmanouil Roditakis, N. E. Roditakis, Anastasia Tsagkarakou
    Abstract:

    The resistance levels to α-cypermethrin, bifenthrin, pirimiphos-methyl, endosulfan and imidacloprid were determined in Bemisia tabaci (Gennadius) from Crete. Five B tabaci populations collected from greenhouse and outdoor crops were bioassayed and compared with a reference susceptible strain. Bemisia tabaci collected in a floriculture greenhouse exhibited the highest resistance against all insecticides: at LC50, resistance factors were 23-fold for bifenthrin, 80-fold for α-cypermethrin, 18-fold for pirimiphos-methyl, 58-fold for endosulfan and 730-fold for imidacloprid. A population collected on outdoor melons was more susceptible than the reference strain against all insecticides tested, suggesting the occurrence of local highly susceptible B tabaci populations in 'refugia'. In pairwise comparisons of resistance levels, correlation was observed between the LC50 values of the pyrethroid insecticides bifenthrin and α-cypermethrin.  2005 Society of Chemical Industry

Adam B Dinsdale - One of the best experts on this subject based on the ideXlab platform.

  • Bemisia tabaci a statement of species status
    Annual Review of Entomology, 2011
    Co-Authors: Paul J. De Barro, Laura M. Boykin, Adam B Dinsdale
    Abstract:

    Bemisia tabaci has long been considered a complex species. It rose to global prominence in the 1980s owing to the global invasion by the commonly named B biotype. Since then, the concomitant eruption of a group of plant viruses known as begomoviruses has created considerable management problems in many countries. However, an enduring set of questions remains: Is B. tabaci a complex species or a species complex, what are Bemisia biotypes, and how did all the genetic variability arise? This review considers these issues and concludes that there is now sufficient evidence to state that B. tabaci is not made up of biotypes and that the use of biotype in this context is erroneous and misleading. Instead, B. tabaci is a complex of 11 well-defined high-level groups containing at least 24 morphologically indistinguishable species.

Qingjun Wu - One of the best experts on this subject based on the ideXlab platform.

  • Genome-wide identification and analysis of nuclear receptors genes for lethal screening against Bemisia tabaci Q.
    Pest Management Science, 2020
    Co-Authors: Chao He, Jinjin Liang, Yang Zeng, Shaoli Wang, Qingjun Wu, Youjun Zhang
    Abstract:

    BACKGROUND: Nuclear receptors (NRs) play an essential role in diverse biological processes, such as insect metamorphosis. Here, transcriptome analysis and functional studies were used to determine whether NRs are involved in metamorphosis of whitefly Bemisia tabaci Q, a serious pest to crops, and to find some potential insecticide targets. RESULTS: Twenty NRs were identified in the Bemisia tabaci Q genome and categorized into the NR0-NR6 subfamilies. The phylogenetic tree of NRs from Bemisia tabaci Q and other representative species was constructed, which provided evolutionary insight into their genetic distances. The results of spatiotemporal gene expression indicated that the majority of NR gene expression was higher in the head than the abdomen and higher in eggs than adults. Further functional analysis using RNA interference (RNAi) showed that NR genes play an important role in Bemisia tabaci Q pupation and eclosion. With respect to high mortality and effects on growth, this was reflected in the unable to become pupa when the third-stage nymph treated with double-stranded RNA (dsRNA) and the developmental time delay (4-7 days) when pupae were treated with dsRNA for the 12 NR genes during molting compared with the development time in the control. CONCLUSION: This study provides insight into NR functions during the metamorphosis stages of Bemisia tabaci Q. Several candidate genes could be potential insecticide targets for whitefly pest control due to their important roles in insect development. (c) 2020 Society of Chemical Industry.

  • Glutathione S-transferases are involved in thiamethoxam resistance in the field whitefly Bemisia tabaci Q (Hemiptera: Aleyrodidae).
    Pesticide Biochemistry and Physiology, 2016
    Co-Authors: Xin Yang, Chao He, Shaoli Wang, Ze-zong Yang, Qingjun Wu
    Abstract:

    The whitefly, Bemisia tabaci, has developed a high level of resistance to thiamethoxam, a second generation neonicotinoid insecticide that has been widely used to control this pest. In this study, we assessed the level of cross-resistance, the activities of detoxifying enzymes, and the expression profiles of 23 glutathione S-transferase (GST) genes in a thiamethoxam-resistant ant and -susceptible strain of Bemisia tabaci Q. The thiamethoxam-resistant strain showed a moderate level of cross-resistance to another nicotinoid insecticide imidacloprid, a low level of cross-resistance to acetamiprid and nitenpyram, and no significant cross-resistance to abamectin and bifenthrin. Among detoxifying enzymes, only GSTs had significantly higher activity in the resistant strain than in the susceptible strain. Seven of 23 GST genes were over-expressed in the resistant strain relative to the susceptible strain. Using the technology of RNA interference to knockdown a GST gene (GST14), the results showed that silencing GST14 increased the mortality of whiteflies to thiamethoxam in Bemisia tabaci.

  • Transmission of Tomato Yellow Leaf Curl Virus by Bemisia tabaci as Affected by Whitefly Sex and Biotype
    Scientific Reports, 2015
    Co-Authors: Wenxi Ning, Yang Zeng, Shaoli Wang, Qingjun Wu
    Abstract:

    Transmission of Tomato Yellow Leaf Curl Virus by Bemisia tabaci as Affected by Whitefly Sex and Biotype