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Pinto, Ruy Eugénio - One of the best experts on this subject based on the ideXlab platform.
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Ensaios densitométricos para a avaliação, ao longo de um ciclo anual, d e alcalóides solúveis em água de raízes de Chelidonium majus L. (Papaveracea), nos Açores
Universidade dos Açores, 1995Co-Authors: Pavão, Maria Leonor, Pinto, Ruy EugénioAbstract:Prepararam-se extractos de alcalóides solúveis em água de raízes de Chelidonium majus L. (celidónia), colhida em terrenos incultos nos Açores, mensalmente, ao longo de um ano. A separação dos alcalóides por cromatografia em camada fina mostrou que a quelidonona, queleritrina, sanguinarina, berberina, coptisina, protopina e alocriptopina são constituintes permanentes dos extractos. Utilizou-se a técnica de fluorescência (366 nm), julgado como o mais adequado, para a quantificação densitométrica da quelidonina, berberina e coptisina. A queleritrina e a sanguinarina só foram avaliadas por fluorescência. Nos casos da quelidonina, berberina e coptisina, é também discutida a utilização do modo de absorção (a 242 nm, 344 nm e 268 nm, respectivamente). A quelidonina revelou ser o constituinte principal (cerca de 70% do total dos alcalóides avaliados) em todos os extractos; os valores máximos da sua concentração surgiram em Julho (60% mais alto do que o valor médio) e o mínimo em Janeiro (60% mais baixo). As concentrações da queleritrina e da sanguinarina foram mais elevadas no inverno (cerca do dobro) e mínimas em Junho (cerca de metade). A berberina e a coptisina mostraram variações de concentração relativamente pequenas. As concentrações de ambas foram máximas em Julho, sendo a da coptisina mínima em Dezembro.ABSTRACT: Every 4 weeks, during a one year cycle, water soluble alkaloid extracts were prepared from Chelidonium majus, L (great celandine) roots, growing spontaneously on uncultivated ground in the Azores. TLC analysis showed that chelidonine, chelerythrine, sanguinarine. Berberine, coptisine, protopine and allocryptopine are present in all the extracts. Fluorescence densitometry (366 nm) was found to be the most adequate densitometric technic to quantify chelidonine, Berberine and coptisine. Sanguinarine and chelerythrine were analysed only by the fluorescence method. The use of the absorption mode for the evaluation of chelidonine, Berberine and coptisine (at 242 nm, 344 nm and 268 nm. respectively) is also discussed. Chelidonine was the major constituent (about 70% of total quantified alkaloids); maximum values appeared in July (60% higher than mean value) and minimum in January (60% lower). Both chelerythrine and sanguinarine concentrations showed maximum values in winter (about twice) and minimum in June (about one half). Berberine and coptisine exhibited relative small changes. Berberine and coptisine contents were maximum in July. The concentration of coptisine was minimum in December
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Sensibilidade de Bacillus subtilis a extractos de alcalóides solúveis em água de raízes de Chelidonium majus L (Papaveracea) dos Açores
Universidade dos Açores, 1995Co-Authors: Pavão, Maria Leonor, Pinto, Ruy EugénioAbstract:Prepararam-se extractos de alcalóides solúveis em água de raízes de Chelidonium majus L. (celidónia), colhida em terrenos incultos nos Açores. Estes extractos revelaram possuir propriedades antibacterianas em relação a Bacillus subtilis. O efeito dos extractos pareceu ser dez vezes menor do que o da tetraciclina. Para concentrações dos extractos inferiores a 100 μg/disco não foi detectada uma clara sensibilidade da cultura. A quelidonina, a protopina e a alocriptopina não são activos contra Bacillus subtilis. A coptisina, que não é citada na bibliografia como possuindo actividade antibacteriana, revelou actuar sobre Bacillus subtilis de um modo semelhante ao apresentado pela sanguinarina e pela berberina. A queleritrina foi o alcalóide que revelou maior efeito sobre Bacillus subtilis, cerca de 40 a 50 % superior ao da sanguinarina. A sanguinarina e a queleritrina são os alcalóides geralmente considerados como os principais responsáveis pela actividade antibacteriana do látex da quelidónia. Sugere-se que esta responsabilidade seja também atribuída à berberina e à coptisina, pelo menos no que diz respeito à espécie da planta existente nos Açores.ABSTRACT: Water soluble alkaloid (WSA) extracts from Chelidonium majus L (great celandine) roots, growing on uncultivated ground in the Azores, were prepared. The WSA showed antibacterial properties towards Bacillus subtilis. The effect of WSA appeared to be 1/10 of tetracycline. For concentrations lower than 100 μg/disc, no reproducible sensivity was observed. Chelidonine, protopine and allocryptopine had no action against Bacillus subtilis. Coptisine, which is reported as not exhibiting antibacterial activity, showed activity against Bacillus subtilis in a similar way as sanguinarine and Berberine. Chelerythrine was the most active alkaloid (about 40-50% higher than sanguinarine). Sanguinarine and chelerythrine are generally accepted as the alkaloids responsible for the antibacterial properties of Chelidonium latex. Results suggest that, at least for the species existing in the Azores, Berberine and coptisine also contribute to that biological activity
Weijian Hang - One of the best experts on this subject based on the ideXlab platform.
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Image_2_Berberine Ameliorates High Glucose-Induced Cardiomyocyte Injury via AMPK Signaling Activation to Stimulate Mitochondrial Biogenesis and Restore Autophagic Flux.TIF
2018Co-Authors: Weijian Hang, Jiehui Chen, Liangtao Xia, Bing Wen, Tao Liang, Xu Wang, Qianying Zhang, Qingjie ChenAbstract:Background: Type II diabetes (T2D)-induced cardiomyocyte hypertrophy is closely linked to the impairment of mitochondrial function. Berberine has been shown to be a promising effect for hypoglycemia in T2D models. High glucose-induced cardiomyocyte hypertrophy in vitro has been reported. The present study investigated the protective effect and the underlying mechanism of Berberine on high glucose-induced H9C2 cell line.Methods: High glucose-induced H9C2 cell line was used to mimic the hyperglycemia resulting in cardiomyocyte hypertrophy. Berberine was used to rescue in this model and explore the mechanism in it. Confocal microscopy, immunofluorescence, RT-PCR, and western blot analysis were performed to evaluate the protective effects of Berberine in high glucose-induced H9C2 cell line.Results: Berberine dramatically alleviated hypertrophy of H9C2 cell line and significantly ameliorated mitochondrial function by rectifying the imbalance of fusion and fission in mitochondrial dynamics. Furthermore, Berberine further promoted mitogenesis and cleared the damaged mitochondria via mitophagy. In addition, Berberine also restored autophagic flux in high glucose-induced cardiomyocyte injury via AMPK signaling pathway activation.Conclusion: Berberine ameliorates high glucose-induced cardiomyocyte injury via AMPK signaling pathway activation to stimulate mitochondrial biogenesis and restore autophagicflux in H9C2 cell line.
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Table_1_Berberine Ameliorates High Glucose-Induced Cardiomyocyte Injury via AMPK Signaling Activation to Stimulate Mitochondrial Biogenesis and Restore Autophagic Flux.DOCX
2018Co-Authors: Weijian Hang, Jiehui Chen, Liangtao Xia, Bing Wen, Tao Liang, Xu Wang, Qianying Zhang, Qingjie ChenAbstract:Background: Type II diabetes (T2D)-induced cardiomyocyte hypertrophy is closely linked to the impairment of mitochondrial function. Berberine has been shown to be a promising effect for hypoglycemia in T2D models. High glucose-induced cardiomyocyte hypertrophy in vitro has been reported. The present study investigated the protective effect and the underlying mechanism of Berberine on high glucose-induced H9C2 cell line.Methods: High glucose-induced H9C2 cell line was used to mimic the hyperglycemia resulting in cardiomyocyte hypertrophy. Berberine was used to rescue in this model and explore the mechanism in it. Confocal microscopy, immunofluorescence, RT-PCR, and western blot analysis were performed to evaluate the protective effects of Berberine in high glucose-induced H9C2 cell line.Results: Berberine dramatically alleviated hypertrophy of H9C2 cell line and significantly ameliorated mitochondrial function by rectifying the imbalance of fusion and fission in mitochondrial dynamics. Furthermore, Berberine further promoted mitogenesis and cleared the damaged mitochondria via mitophagy. In addition, Berberine also restored autophagic flux in high glucose-induced cardiomyocyte injury via AMPK signaling pathway activation.Conclusion: Berberine ameliorates high glucose-induced cardiomyocyte injury via AMPK signaling pathway activation to stimulate mitochondrial biogenesis and restore autophagicflux in H9C2 cell line.
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Berberine Ameliorates High Glucose-Induced Cardiomyocyte Injury via AMPK Signaling Activation to Stimulate Mitochondrial Biogenesis and Restore Autophagic Flux
Frontiers Media S.A., 2018Co-Authors: Weijian Hang, Jiehui Chen, Liangtao Xia, Bing Wen, Tao Liang, Xu Wang, Qianying ZhangAbstract:Background: Type II diabetes (T2D)-induced cardiomyocyte hypertrophy is closely linked to the impairment of mitochondrial function. Berberine has been shown to be a promising effect for hypoglycemia in T2D models. High glucose-induced cardiomyocyte hypertrophy in vitro has been reported. The present study investigated the protective effect and the underlying mechanism of Berberine on high glucose-induced H9C2 cell line.Methods: High glucose-induced H9C2 cell line was used to mimic the hyperglycemia resulting in cardiomyocyte hypertrophy. Berberine was used to rescue in this model and explore the mechanism in it. Confocal microscopy, immunofluorescence, RT-PCR, and western blot analysis were performed to evaluate the protective effects of Berberine in high glucose-induced H9C2 cell line.Results: Berberine dramatically alleviated hypertrophy of H9C2 cell line and significantly ameliorated mitochondrial function by rectifying the imbalance of fusion and fission in mitochondrial dynamics. Furthermore, Berberine further promoted mitogenesis and cleared the damaged mitochondria via mitophagy. In addition, Berberine also restored autophagic flux in high glucose-induced cardiomyocyte injury via AMPK signaling pathway activation.Conclusion: Berberine ameliorates high glucose-induced cardiomyocyte injury via AMPK signaling pathway activation to stimulate mitochondrial biogenesis and restore autophagicflux in H9C2 cell line
Li Chen - One of the best experts on this subject based on the ideXlab platform.
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Berberine enhances the ampk activation and autophagy and mitigates high glucose induced apoptosis of mouse podocytes
European Journal of Pharmacology, 2017Co-Authors: Yingli Jin, Shuping Liu, Dong Xiao, Li ChenAbstract:High glucose concentration can induce injury of podocytes and Berberine has a potent activity against diabetic nephropathy. However, whether and how Berberine can inhibit high glucose-mediated injury of podocytes have not been clarified. This study tested the effect of Berberine on high glucose-mediated apoptosis and the AMP-activated protein kinase (AMPK), mammalian target of rapamycin (mTOR) activation and autophagy in podocytes. The results indicated that Berberine significantly mitigated high glucose-decreased cell viability, and nephrin and podocin expression as well as apoptosis in mouse podocytes. Berberine significantly increased the AMPK activation and mitigated high glucose and/or the AMPK inhibitor, compound C-mediated mTOR activation and apoptosis in podocytes. Berberine significantly enhanced the AMPK activation and protected from high glucose-induced apoptosis in the AMPK-silencing podocytes. Furthermore, Berberine significantly increased the high glucose-elevated Unc-51-like autophagy-activating kinase 1 (ULK1) S317/S555 phosphorylation, Beclin-1 expression, the ratios of LC3II to LC3I expression and the numbers of autophagosomes, but reduced ULK1 S757 phosphorylation in podocytes. In addition, Berberine significantly attenuated compound C-mediated inhibition of autophagy in podocytes. The protective effect of Berberine on high glucose-induced podocyte apoptosis was significantly mitigated by pre-treatment with 3-methyladenine or bafilomycin A1. Collectively, Berberine enhanced autophagy and protected from high glucose-induced injury in podocytes by promoting the AMPK activation. Our findings may provide new insights into the molecular mechanisms underlying the anti-diabetic nephropathy effect of Berberine and may aid in design of new therapies for intervention of diabetic nephropathy.
Qingjie Chen - One of the best experts on this subject based on the ideXlab platform.
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Image_2_Berberine Ameliorates High Glucose-Induced Cardiomyocyte Injury via AMPK Signaling Activation to Stimulate Mitochondrial Biogenesis and Restore Autophagic Flux.TIF
2018Co-Authors: Weijian Hang, Jiehui Chen, Liangtao Xia, Bing Wen, Tao Liang, Xu Wang, Qianying Zhang, Qingjie ChenAbstract:Background: Type II diabetes (T2D)-induced cardiomyocyte hypertrophy is closely linked to the impairment of mitochondrial function. Berberine has been shown to be a promising effect for hypoglycemia in T2D models. High glucose-induced cardiomyocyte hypertrophy in vitro has been reported. The present study investigated the protective effect and the underlying mechanism of Berberine on high glucose-induced H9C2 cell line.Methods: High glucose-induced H9C2 cell line was used to mimic the hyperglycemia resulting in cardiomyocyte hypertrophy. Berberine was used to rescue in this model and explore the mechanism in it. Confocal microscopy, immunofluorescence, RT-PCR, and western blot analysis were performed to evaluate the protective effects of Berberine in high glucose-induced H9C2 cell line.Results: Berberine dramatically alleviated hypertrophy of H9C2 cell line and significantly ameliorated mitochondrial function by rectifying the imbalance of fusion and fission in mitochondrial dynamics. Furthermore, Berberine further promoted mitogenesis and cleared the damaged mitochondria via mitophagy. In addition, Berberine also restored autophagic flux in high glucose-induced cardiomyocyte injury via AMPK signaling pathway activation.Conclusion: Berberine ameliorates high glucose-induced cardiomyocyte injury via AMPK signaling pathway activation to stimulate mitochondrial biogenesis and restore autophagicflux in H9C2 cell line.
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Table_1_Berberine Ameliorates High Glucose-Induced Cardiomyocyte Injury via AMPK Signaling Activation to Stimulate Mitochondrial Biogenesis and Restore Autophagic Flux.DOCX
2018Co-Authors: Weijian Hang, Jiehui Chen, Liangtao Xia, Bing Wen, Tao Liang, Xu Wang, Qianying Zhang, Qingjie ChenAbstract:Background: Type II diabetes (T2D)-induced cardiomyocyte hypertrophy is closely linked to the impairment of mitochondrial function. Berberine has been shown to be a promising effect for hypoglycemia in T2D models. High glucose-induced cardiomyocyte hypertrophy in vitro has been reported. The present study investigated the protective effect and the underlying mechanism of Berberine on high glucose-induced H9C2 cell line.Methods: High glucose-induced H9C2 cell line was used to mimic the hyperglycemia resulting in cardiomyocyte hypertrophy. Berberine was used to rescue in this model and explore the mechanism in it. Confocal microscopy, immunofluorescence, RT-PCR, and western blot analysis were performed to evaluate the protective effects of Berberine in high glucose-induced H9C2 cell line.Results: Berberine dramatically alleviated hypertrophy of H9C2 cell line and significantly ameliorated mitochondrial function by rectifying the imbalance of fusion and fission in mitochondrial dynamics. Furthermore, Berberine further promoted mitogenesis and cleared the damaged mitochondria via mitophagy. In addition, Berberine also restored autophagic flux in high glucose-induced cardiomyocyte injury via AMPK signaling pathway activation.Conclusion: Berberine ameliorates high glucose-induced cardiomyocyte injury via AMPK signaling pathway activation to stimulate mitochondrial biogenesis and restore autophagicflux in H9C2 cell line.
Tomoo Hosoe - One of the best experts on this subject based on the ideXlab platform.
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11 hydroxylation of protoBerberine by the novel Berberine utilizing aerobic bacterium sphingobium sp strain bd3100
Journal of Natural Products, 2015Co-Authors: Hisashi Takeda, Kazuki Ishikawa, Daigo Wakana, Masao Fukuda, Fumihiko Sato, Tomoo HosoeAbstract:ProtoBerberine alkaloids, including Berberine, palmatine, and berberrubine, are produced by medicinal plants and are known to have various pharmacological effects. We isolated two Berberine-utilizing bacteria, Sphingobium sp. strain BD3100 and Rhodococcus sp. strain BD7100, from soil collected at a natural medicine factory. BD3100 had the unique ability to utilize Berberine or palmatine as the sole carbon and energy source. BD3100 produced demethyleneBerberine in Berberine-supplemented medium. In a resting-cell incubation with Berberine, BD3100 produced 11-hydroxyBerberine; the structure of 11-hydroxyBerberine was determined by detailed analysis of NMR and MS spectroscopic data. α-Naphthoflavone, miconazole, and ketoconazole, which are known inhibitors of cytochrome P450, interfered with BD3100 metabolism of Berberine in resting cells. Inhibition by miconazole led to the production of a new compound, 11-hydroxydemethyleneBerberine. In a resting-cell incubation with palmatine, BD3100 generated 11-hydroxypalmatine. This work represents the first report of the isolation and characterization of novel Berberine-utilizing aerobic bacteria for the production of 11-hydroxylation derivatives of Berberine and palmatine.
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11-Hydroxylation of ProtoBerberine by the Novel Berberine-Utilizing Aerobic Bacterium Sphingobium sp. Strain BD3100
2015Co-Authors: Hisashi Takeda, Kazuki Ishikawa, Daigo Wakana, Masao Fukuda, Fumihiko Sato, Tomoo HosoeAbstract:ProtoBerberine alkaloids, including Berberine, palmatine, and berberrubine, are produced by medicinal plants and are known to have various pharmacological effects. We isolated two Berberine-utilizing bacteria, Sphingobium sp. strain BD3100 and Rhodococcus sp. strain BD7100, from soil collected at a natural medicine factory. BD3100 had the unique ability to utilize Berberine or palmatine as the sole carbon and energy source. BD3100 produced demethyleneBerberine in Berberine-supplemented medium. In a resting-cell incubation with Berberine, BD3100 produced 11-hydroxyBerberine; the structure of 11-hydroxyBerberine was determined by detailed analysis of NMR and MS spectroscopic data. α-Naphthoflavone, miconazole, and ketoconazole, which are known inhibitors of cytochrome P450, interfered with BD3100 metabolism of Berberine in resting cells. Inhibition by miconazole led to the production of a new compound, 11-hydroxydemethyleneBerberine. In a resting-cell incubation with palmatine, BD3100 generated 11-hydroxypalmatine. This work represents the first report of the isolation and characterization of novel Berberine-utilizing aerobic bacteria for the production of 11-hydroxylation derivatives of Berberine and palmatine