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Landoulsi Ameni - One of the best experts on this subject based on the ideXlab platform.
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Antimicrobial and Light‐Enhanced Antimicrobial Activities, Cytotoxicity and Chemical Variability of All Tunisian Eryngium Species
'Wiley', 2020Co-Authors: Landoulsi Ameni, Hennebelle Thierry, Bero Joanne, Rivière Céline, Sahpaz Sevser, Quetin-leclercq Joëlle, Neut Christel, Benhamida Jeannette, Roumy VincentAbstract:International audienceThis study was performed on all Eryngium species growing in Tunisia in order to evaluate their intra and interspecies variabilities and to investigate their biological activities. These species are used in traditional medicine, and literature about the phytochemical investigations of most of them is scarce. Antimicrobial and light-enhanced activities were tested against multiresistant microorganisms and extended spectrum beta-lactamase producing bacteria (ESBL). All studied species showed antimicrobial effect with several MIC values lower than 70 μg/ml. Tested Eryngium species have proven to be a promising source of photoactive compounds, while light-enhanced activity offers an alternative for the inactivation of pathogenic microorganisms which is currently subjected to a great interest. This is the first report of this activity in genus Eryngium. A significant improvement of antimicrobial activity with UV irradiation was observed, mainly for E. dichotomum, E. ilicifolium and E. triquetrum. Cytotoxicity, studied for the first time for the most species, was evaluated against cancer (J774) and non-cancer (WI38) human cell lines. Chemical composition of volatile compounds presented in the most active crude extracts (petroleum ether extracts) of the aerial parts was investigated using GC/MS analysis and was submitted to statistical analyses. It revealed their high content of bioactive phytochemicals, particularly oxygenated sesquiterpenes like spathulenol, ledol and α-bisabolol but also hydrocarbon sesquiterpenes such as β-bisabolene and copaene, as well as polyacetylene derivatives such as falcarinol. Statistical analyses permitted to evaluate the interrelations between all Tunisian Eryngium species
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Antimicrobial and Light-Enhanced Antimicrobial Activities, Cytotoxicity and Chemical Variability of All Tunisian Eryngium Species.
'Wiley', 2020Co-Authors: Landoulsi Ameni, Hennebelle Thierry, Bero Joanne, Rivière Céline, Sahpaz Sevser, Quetin-leclercq Joëlle, Neut Christel, Benhamida Jeannette, Roumy VincentAbstract:This study was performed on all Eryngium species growing in Tunisia in order to evaluate their intra and interspecies variabilities and to investigate their biological activities. These species are used in traditional medicine, and literature about the phytochemical investigations of most of them is scarce. Antimicrobial and light-enhanced activities were tested against multiresistant microorganisms and extended spectrum beta-lactamase producing bacteria (ESBL). All studied species showed antimicrobial effect with several MIC values lower than 70 μg/ml. Tested Eryngium species have proven to be a promising source of photoactive compounds, while light-enhanced activity offers an alternative for the inactivation of pathogenic microorganisms which is currently subjected to a great interest. This is the first report of this activity in genus Eryngium. A significant improvement of antimicrobial activity with UV irradiation was observed, mainly for E. dichotomum, E. ilicifolium and E. triquetrum. Cytotoxicity, studied for the first time for the most species, was evaluated against cancer (J774) and non-cancer (WI38) human cell lines. Chemical composition of volatile compounds presented in the most active crude extracts (petroleum ether extracts) of the aerial parts was investigated using GC/MS analysis and was submitted to statistical analyses. It revealed their high content of bioactive phytochemicals, particularly oxygenated sesquiterpenes like spathulenol, ledol and α-bisabolol but also hydrocarbon sesquiterpenes such as β-bisabolene and copaene, as well as polyacetylene derivatives such as falcarinol. Statistical analyses permitted to evaluate the interrelations between all Tunisian Eryngium species
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Etude chimiotaxonomique et activité biologique des métabolites secondaires des plantes du genre Eryngium
HAL CCSD, 2016Co-Authors: Landoulsi AmeniAbstract:The genus Eryngium L., (Apiaceae, Saniculoideae) comprises more than 250 flowering plant species, which are commonly used as medicinal plants in different countries. Only eight species are growing in Tunisia: E. barrelieri Boiss., E. campestre L., E. dichotomum Desf., E. glomeratum Lamk., E. ilicifolium Lamk., E. maritimum L., E. tricuspidatum L. et E. triquetrum Vahl. These species are used in traditional medicine and there are relatively few papers on the phytochemical investigations of most of them. This study was performed on all Eryngium species growing in Tunisia in order to evaluate their chemical variability using GC-FID and GC-MS analyses and their biological activities, mainly antimicrobial against multiresistant microorganisms and extended spectrum beta-lactamase producing bacteria (ESBL), and also phototoxic and cytotoxic effects.All investigated species showed considerable antimicrobial effect with MIC value ranging between 1,25 and 0,07 mg/mL and important cytotoxic activity against J774 tumoral cells with IC50 from 24,4 to 0,32 µg/mL. Phototoxic investigation demonstrated a significant photoactive inhibitory effects against tested pathogenic microorganisms.GC–MS analysis of the most active crude extracts (petroleum ether extracts) revealed their high content of antimicrobial agents particularly oxygenated sesquiterpenes such as spathulenol, ledol, α-bisabolol and cubenol, and hydrocarbon sesquiterpenes such as β-bisabolene and copaene; and cytotoxic components such as falcarinol.The chemical investigation and bio-guided isolation of active compounds of the selected crude extract of E. triquetrum roots were performed using column chromatography, centrifugal partition chromatography (CPC) and preparative high-performance liquid chromatography (HPLC). among purified components, two bioactive polyacetylenes, panaxydiol and falcarinol, showed a great antimicrobial activity mainly against multiresistant and ESBL producing Pseudomonas aeruginosa with MIC value up to 0,25 ng/ml and a mostly bactericidal effect.Le genre Eryngium L. (Apiaceae, Saniculoideae) comprend plus de 250 espèces utilisées en médecine traditionnelle à travers le monde. En Tunisie il existe seulement huit espèces : E. barrelieri Boiss., E. campestre L., E. dichotomum Desf., E. glomeratum Lamk., E. ilicifolium Lamk., E. maritimum L., E. tricuspidatum L. et E. triquetrum Vahl. ; ces espèces sont en majorité peu étudiées du point de vue phytochimique. La présente étude a été effectuée sur la totalité des Eryngium qui poussent en Tunisie dans le but d’évaluer leurs activités biologiques, essentiellement antimicrobienne contre des microorganismes multirésistants et producteurs de béta-lactamases à spectres étendus (BLSE), mais aussi phototoxique et cytotoxique ainsi que la variabilité chimique par analyse par GC-FID et GC-MS des extraits les plus actifs.Toutes les espèces étudiées étaient dotées d’un pouvoir antimicrobien (1,25 à 0,07 mg/mL) et cytotoxique (24,4 à 0,32 µg/mL) considérable. Le criblage de l’activité phototoxique a permis de mettre en évidence la richesse de ces plantes en composés photoréactifs antimicrobiens potentiellement intéressants pour leur efficacité d’action et l’élargissement du spectre d’activité antimicrobienne.L’analyse des extraits actifs apolaires a permis d’étudier la variabilité chimique entre les différentes espèces et la mise en évidence de la présence majoritaire de composés antimicrobiens notamment des sesquiterpènes oxygénés tels que le spathulénol, le lédol, l’α-bisabolol et le cubénol, et des sesquiterpènes hydrocarbonés comme le β-bisabolène et le copaène ; et cytotoxiques tel que le falcarinol.Une étude phytochimique approfondie a été réalisée sur les racines d’E. triquetrum afin d’extraire, isoler par des essais bio-guidés et identifier les composés actifs. Le fractionnement a été optimisé par des chromatographies sur colonnes, CPC et CLHP. Parmi les composés identifiés deux polyacétylènes, le panaxydiol et le falcarinol, ont montré un fort pouvoir antimicrobien et une spécificité d’action notamment contre les souches de Pseudomonas aeruginosa BLSE et multirésistantes, avec des CMI allant jusqu’à 0,25 ng/mL et une activité en majorité bactéricide
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Chemotaxonomic study and biological activity of secondary metabolites of Eryngium species
2016Co-Authors: Landoulsi AmeniAbstract:Le genre Eryngium L. (Apiaceae, Saniculoideae) comprend plus de 250 espèces utilisées en médecine traditionnelle à travers le monde. En Tunisie il existe seulement huit espèces : E. barrelieri Boiss., E. campestre L., E. dichotomum Desf., E. glomeratum Lamk., E. ilicifolium Lamk., E. maritimum L., E. tricuspidatum L. et E. triquetrum Vahl. ; ces espèces sont en majorité peu étudiées du point de vue phytochimique. La présente étude a été effectuée sur la totalité des Eryngium qui poussent en Tunisie dans le but d’évaluer leurs activités biologiques, essentiellement antimicrobienne contre des microorganismes multirésistants et producteurs de béta-lactamases à spectres étendus (BLSE), mais aussi phototoxique et cytotoxique ainsi que la variabilité chimique par analyse par GC-FID et GC-MS des extraits les plus actifs.Toutes les espèces étudiées étaient dotées d’un pouvoir antimicrobien (1,25 à 0,07 mg/mL) et cytotoxique (24,4 à 0,32 µg/mL) considérable. Le criblage de l’activité phototoxique a permis de mettre en évidence la richesse de ces plantes en composés photoréactifs antimicrobiens potentiellement intéressants pour leur efficacité d’action et l’élargissement du spectre d’activité antimicrobienne.L’analyse des extraits actifs apolaires a permis d’étudier la variabilité chimique entre les différentes espèces et la mise en évidence de la présence majoritaire de composés antimicrobiens notamment des sesquiterpènes oxygénés tels que le spathulénol, le lédol, l’α-bisabolol et le cubénol, et des sesquiterpènes hydrocarbonés comme le β-bisabolène et le copaène ; et cytotoxiques tel que le falcarinol.Une étude phytochimique approfondie a été réalisée sur les racines d’E. triquetrum afin d’extraire, isoler par des essais bio-guidés et identifier les composés actifs. Le fractionnement a été optimisé par des chromatographies sur colonnes, CPC et CLHP. Parmi les composés identifiés deux polyacétylènes, le panaxydiol et le falcarinol, ont montré un fort pouvoir antimicrobien et une spécificité d’action notamment contre les souches de Pseudomonas aeruginosa BLSE et multirésistantes, avec des CMI allant jusqu’à 0,25 ng/mL et une activité en majorité bactéricide.The genus Eryngium L., (Apiaceae, Saniculoideae) comprises more than 250 flowering plant species, which are commonly used as medicinal plants in different countries. Only eight species are growing in Tunisia: E. barrelieri Boiss., E. campestre L., E. dichotomum Desf., E. glomeratum Lamk., E. ilicifolium Lamk., E. maritimum L., E. tricuspidatum L. et E. triquetrum Vahl. These species are used in traditional medicine and there are relatively few papers on the phytochemical investigations of most of them. This study was performed on all Eryngium species growing in Tunisia in order to evaluate their chemical variability using GC-FID and GC-MS analyses and their biological activities, mainly antimicrobial against multiresistant microorganisms and extended spectrum beta-lactamase producing bacteria (ESBL), and also phototoxic and cytotoxic effects.All investigated species showed considerable antimicrobial effect with MIC value ranging between 1,25 and 0,07 mg/mL and important cytotoxic activity against J774 tumoral cells with IC50 from 24,4 to 0,32 µg/mL. Phototoxic investigation demonstrated a significant photoactive inhibitory effects against tested pathogenic microorganisms.GC–MS analysis of the most active crude extracts (petroleum ether extracts) revealed their high content of antimicrobial agents particularly oxygenated sesquiterpenes such as spathulenol, ledol, α-bisabolol and cubenol, and hydrocarbon sesquiterpenes such as β-bisabolene and copaene; and cytotoxic components such as falcarinol.The chemical investigation and bio-guided isolation of active compounds of the selected crude extract of E. triquetrum roots were performed using column chromatography, centrifugal partition chromatography (CPC) and preparative high-performance liquid chromatography (HPLC). among purified components, two bioactive polyacetylenes, panaxydiol and falcarinol, showed a great antimicrobial activity mainly against multiresistant and ESBL producing Pseudomonas aeruginosa with MIC value up to 0,25 ng/ml and a mostly bactericidal effect
Ligia Salgueiro - One of the best experts on this subject based on the ideXlab platform.
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chemical composition and biological activity of the volatile extracts of achillea millefolium
Natural Product Communications, 2011Co-Authors: Danilo Falconieri, Bruno Marongiu, Alessandra Piras, Silvia Porcedda, Maria Jose Goncalves, Carlos Cavaleiro, Celia Cabral, Ligia SalgueiroAbstract:: In this study, flowering aerial parts of wild Achillea millefolium growing on the Mediterranean coast (Sardinia Island, Italy) and on the Atlantic coast (Portugal- Serra de Montemuro) were used as a matrix for supercritical extraction of volatile oil with CO2 (SFE). The collected extracts were analyzed by GC-FID and GC-MS methods and their composition were compared with that of the essential oil isolated by hydrodistillation. A strong chemical variability in essential oils depending on the origin of the samples was observed. The results showed the presence of two type oils. The Italian volatile extracts (SFE and essential oil) are predominantly composed by alpha-asarone (25.6-33.3%, in the SFE extract and in the HD oil, respectively), Beta-Bisabolene (27.3-16.6%) and alpha-pinene (10.0-17.0%); whereas the main components of the Portuguese extracts are trans-thujone (31.4-29.0%), trans-crhysanthenyl acetate (19.8-15.8%) and beta-pinene (1.2-11.1%). The minimal inhibitory concentration (MIC) and the minimal lethal concentration (MLC) were used to evaluate the antifungal activity of the oils against Candida albicans, C. tropicalis, C. krusei, C. guillermondii, C. parapsilosis, Cryptococcus neoformans, Trichophyton rubrum, T. mentagrophytes, T. mentagrophytes var. interdigitale, T. verrucosum, Microsporum canis, M. gypseum, Epidermophyton floccosum, Aspergillus niger, A. fumigatus and A. flavus. The oils showed the highest activity against dermatophyte strains, with MIC values ranging from 0.32-1.25 microL mL(-1).
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chemical characterization and biological activity of essential oils from daucus carota l subsp carota growing wild on the mediterranean coast and on the atlantic coast
Fitoterapia, 2009Co-Authors: Andrea Maxia, Bruno Marongiu, Alessandra Piras, Silvia Porcedda, Enrica Tuveri, Maria Jose Goncalves, Carlos Cavaleiro, Ligia SalgueiroAbstract:The essential oils and supercritical CO(2) extracts of wild Daucus carota L. subsp. carota growing spontaneously in Sardinia and in Portugal were investigated. The main components in the Sardinian essential oil of flowering and mature umbels with seeds are Beta-Bisabolene (17.6-51.0%) and 11-alpha-(H)-himachal-4-en-1-beta-ol (9.0-21.6%); instead, the oils from Portuguese samples are predominantly composed of geranyl acetate (5.2-65.0%) and alpha-pinene (3.5-37.9%). Supercritical extracts contain lower amounts of monoterpenes and higher amounts of sesquiterpene hydrocarbons. Antifungal activities of the Sardinian oils were the highest, particularly for dermatophytes and Cryptococcus neoformans, with MIC values of 0.16-0.64 microL mL(-1).
Roumy Vincent - One of the best experts on this subject based on the ideXlab platform.
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Antimicrobial and Light‐Enhanced Antimicrobial Activities, Cytotoxicity and Chemical Variability of All Tunisian Eryngium Species
'Wiley', 2020Co-Authors: Landoulsi Ameni, Hennebelle Thierry, Bero Joanne, Rivière Céline, Sahpaz Sevser, Quetin-leclercq Joëlle, Neut Christel, Benhamida Jeannette, Roumy VincentAbstract:International audienceThis study was performed on all Eryngium species growing in Tunisia in order to evaluate their intra and interspecies variabilities and to investigate their biological activities. These species are used in traditional medicine, and literature about the phytochemical investigations of most of them is scarce. Antimicrobial and light-enhanced activities were tested against multiresistant microorganisms and extended spectrum beta-lactamase producing bacteria (ESBL). All studied species showed antimicrobial effect with several MIC values lower than 70 μg/ml. Tested Eryngium species have proven to be a promising source of photoactive compounds, while light-enhanced activity offers an alternative for the inactivation of pathogenic microorganisms which is currently subjected to a great interest. This is the first report of this activity in genus Eryngium. A significant improvement of antimicrobial activity with UV irradiation was observed, mainly for E. dichotomum, E. ilicifolium and E. triquetrum. Cytotoxicity, studied for the first time for the most species, was evaluated against cancer (J774) and non-cancer (WI38) human cell lines. Chemical composition of volatile compounds presented in the most active crude extracts (petroleum ether extracts) of the aerial parts was investigated using GC/MS analysis and was submitted to statistical analyses. It revealed their high content of bioactive phytochemicals, particularly oxygenated sesquiterpenes like spathulenol, ledol and α-bisabolol but also hydrocarbon sesquiterpenes such as β-bisabolene and copaene, as well as polyacetylene derivatives such as falcarinol. Statistical analyses permitted to evaluate the interrelations between all Tunisian Eryngium species
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Antimicrobial and Light-Enhanced Antimicrobial Activities, Cytotoxicity and Chemical Variability of All Tunisian Eryngium Species.
'Wiley', 2020Co-Authors: Landoulsi Ameni, Hennebelle Thierry, Bero Joanne, Rivière Céline, Sahpaz Sevser, Quetin-leclercq Joëlle, Neut Christel, Benhamida Jeannette, Roumy VincentAbstract:This study was performed on all Eryngium species growing in Tunisia in order to evaluate their intra and interspecies variabilities and to investigate their biological activities. These species are used in traditional medicine, and literature about the phytochemical investigations of most of them is scarce. Antimicrobial and light-enhanced activities were tested against multiresistant microorganisms and extended spectrum beta-lactamase producing bacteria (ESBL). All studied species showed antimicrobial effect with several MIC values lower than 70 μg/ml. Tested Eryngium species have proven to be a promising source of photoactive compounds, while light-enhanced activity offers an alternative for the inactivation of pathogenic microorganisms which is currently subjected to a great interest. This is the first report of this activity in genus Eryngium. A significant improvement of antimicrobial activity with UV irradiation was observed, mainly for E. dichotomum, E. ilicifolium and E. triquetrum. Cytotoxicity, studied for the first time for the most species, was evaluated against cancer (J774) and non-cancer (WI38) human cell lines. Chemical composition of volatile compounds presented in the most active crude extracts (petroleum ether extracts) of the aerial parts was investigated using GC/MS analysis and was submitted to statistical analyses. It revealed their high content of bioactive phytochemicals, particularly oxygenated sesquiterpenes like spathulenol, ledol and α-bisabolol but also hydrocarbon sesquiterpenes such as β-bisabolene and copaene, as well as polyacetylene derivatives such as falcarinol. Statistical analyses permitted to evaluate the interrelations between all Tunisian Eryngium species
António F. Palavra - One of the best experts on this subject based on the ideXlab platform.
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Enrichment of the thymoquinone content in volatile oil from Satureja montana using supercritical fluid extraction.
Journal of separation science, 2009Co-Authors: Clara Grosso, Ana M. Mainar, José S. Urieta, José G. Barroso, Ana Cristina Figueiredo, Jesus Burillo, José A. Coelho, António F. PalavraAbstract:Supercritical fluid extraction (SFE) of the volatile oil from Satureja montana L. was performed under different conditions of pressure (90 and 100 bar), temperature (40 and 50 degrees C), mean particle sizes (0.4, 0.6 and 0.8 mm) and CO(2) flow rate (0.8, 1.1 and 1.3 kg/h) to understand the influence of these parameters on the composition and yield of this oil. The results were compared with those obtained for the essential oil isolated by hydrodistillation (HD). The volatile and the essential oil were analysed by GC and GC-MS. The main compounds are carvacrol (52.2-62.0% for HD vs. 41.7-64.5% for SFE), thymol (8.6-11.0% for HD vs. 6.0-11.3% for SFE), p-cymene (6.9-12.8% for HD vs. 6.0-17.8% for SFE), gamma-terpinene (6.4-9.4% for HD vs. 2.3-6.0% for SFE) and Beta-Bisabolene (2.0-2.7% for HD vs. 2.2-3.5% for SFE). The major difference between SFE and HD was the relative amount of thymoquinone, an oxygenated monoterpene with important biological activities, which can be ten-fold higher in volatile oil (1.6-3.0 for SFE vs. 0.2% for HD). The morphology of the glandular trichomes of S. montana and the effect of the grinding process on them was also evaluated by SEM.
Silvia Porcedda - One of the best experts on this subject based on the ideXlab platform.
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chemical composition and biological activity of the volatile extracts of achillea millefolium
Natural Product Communications, 2011Co-Authors: Danilo Falconieri, Bruno Marongiu, Alessandra Piras, Silvia Porcedda, Maria Jose Goncalves, Carlos Cavaleiro, Celia Cabral, Ligia SalgueiroAbstract:: In this study, flowering aerial parts of wild Achillea millefolium growing on the Mediterranean coast (Sardinia Island, Italy) and on the Atlantic coast (Portugal- Serra de Montemuro) were used as a matrix for supercritical extraction of volatile oil with CO2 (SFE). The collected extracts were analyzed by GC-FID and GC-MS methods and their composition were compared with that of the essential oil isolated by hydrodistillation. A strong chemical variability in essential oils depending on the origin of the samples was observed. The results showed the presence of two type oils. The Italian volatile extracts (SFE and essential oil) are predominantly composed by alpha-asarone (25.6-33.3%, in the SFE extract and in the HD oil, respectively), Beta-Bisabolene (27.3-16.6%) and alpha-pinene (10.0-17.0%); whereas the main components of the Portuguese extracts are trans-thujone (31.4-29.0%), trans-crhysanthenyl acetate (19.8-15.8%) and beta-pinene (1.2-11.1%). The minimal inhibitory concentration (MIC) and the minimal lethal concentration (MLC) were used to evaluate the antifungal activity of the oils against Candida albicans, C. tropicalis, C. krusei, C. guillermondii, C. parapsilosis, Cryptococcus neoformans, Trichophyton rubrum, T. mentagrophytes, T. mentagrophytes var. interdigitale, T. verrucosum, Microsporum canis, M. gypseum, Epidermophyton floccosum, Aspergillus niger, A. fumigatus and A. flavus. The oils showed the highest activity against dermatophyte strains, with MIC values ranging from 0.32-1.25 microL mL(-1).
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chemical characterization and biological activity of essential oils from daucus carota l subsp carota growing wild on the mediterranean coast and on the atlantic coast
Fitoterapia, 2009Co-Authors: Andrea Maxia, Bruno Marongiu, Alessandra Piras, Silvia Porcedda, Enrica Tuveri, Maria Jose Goncalves, Carlos Cavaleiro, Ligia SalgueiroAbstract:The essential oils and supercritical CO(2) extracts of wild Daucus carota L. subsp. carota growing spontaneously in Sardinia and in Portugal were investigated. The main components in the Sardinian essential oil of flowering and mature umbels with seeds are Beta-Bisabolene (17.6-51.0%) and 11-alpha-(H)-himachal-4-en-1-beta-ol (9.0-21.6%); instead, the oils from Portuguese samples are predominantly composed of geranyl acetate (5.2-65.0%) and alpha-pinene (3.5-37.9%). Supercritical extracts contain lower amounts of monoterpenes and higher amounts of sesquiterpene hydrocarbons. Antifungal activities of the Sardinian oils were the highest, particularly for dermatophytes and Cryptococcus neoformans, with MIC values of 0.16-0.64 microL mL(-1).