The Experts below are selected from a list of 285 Experts worldwide ranked by ideXlab platform

Antonio L. Crego - One of the best experts on this subject based on the ideXlab platform.

Kati Hanhineva - One of the best experts on this subject based on the ideXlab platform.

  • Amino acid-derived Betaines dominate as urinary markers for rye bran intake in mice fed high-fat diet--A nontargeted metabolomics study.
    Molecular nutrition & food research, 2015
    Co-Authors: Jenna Pekkinen, Hannu Mykkänen, Pekka Keski-rahkonen, Kaisa Poutanen, Valérie Micard, Natalia Rosa-sibakov, Marko Lehtonen, Kati Hanhineva
    Abstract:

    Scope Bioprocessing of whole grain cereals may affect the bioavailability of phytochemicals associated with grain fiber and ultimately lead to different health outcomes. Here, we studied the impact of long-term feeding with intact and bioprocessed rye bran on the urinary phytochemical profile of mice. Methods and results Nontargeted hydrophilic interaction chromatography-ESI-qTOF-MS metabolite profiling approach was applied on urine samples collected from three groups of diet-induced obese mice fed for 8 weeks with one of the three diets: high-fat (HF) control diet, HF diet enriched with intact rye bran, or HF diet enriched with bioprocessed rye bran. The most striking finding was the increased urinary excretion of several amino-acid derived Betaines after both rye diets. These included proline Betaine, alanine Betaine, valine Betaine, phenylalanine Betaine, pipecolic acid Betaine, and trigonelline, but not glycine Betaine. Furthermore, bioprocessing may have improved the bioavailability of rye-derived phytochemicals, as higher increase in, e.g. ferulic acid and benzoxazinoid metabolites were observed in urine of mice fed with bioprocessed than intact rye bran. Conclusion Urinary excretion of various Betaines was greatly increased in mice fed rye brans. Furthermore, bioprocessing of rye bran appears to serve as a beneficial way to improve the bioavailability of various phytochemicals.

  • Amino acid-derived Betaines dominate as urinary markers for rye bran intake in mice fed high-fat diet – a non-targeted metabolomics study
    Molecular Nutrition and Food Research, 2015
    Co-Authors: Jenna Pekkinen, Hannu Mykkänen, Pekka Keski-rahkonen, Kaisa Poutanen, Valérie Micard, Marko Lehtonen, Natalia Rosa, Kati Hanhineva
    Abstract:

    Scope : Bioprocessing of whole grain cereals may affect the bioavailability of phytochemicals associated with grain fiber and ultimately lead to different health outcomes. Here, we studied the impact of long-term feeding with intact and bioprocessed rye bran on the urinary phytochemical profile of mice. Methods and results : Non-targeted HILIC-ESI-qTOF-MS metabolite profiling approach was applied on urine samples collected from 3 groups of diet-induced obese mice fed for 8 weeks with one of the three diets: high-fat (HF) control diet, HF diet enriched with intact rye bran, or HF diet enriched with bioprocessed rye bran. The most striking finding was the increased urinary excretion of several amino-acid derived Betaines after both rye diets. These included proline Betaine, alanine Betaine, valine Betaine, phenylalanine Betaine, pipecolic acid Betaine, and trigonelline, but not glycine Betaine. Furthermore, bioprocessing may have improved the bioavailability of rye derived phytochemicals, as higher increase in e.g. ferulic acid and benzoxazinoid metabolites were observed in urine of mice fed with bioprocessed than intact rye bran. Conclusion : Urinary excretion of various Betaines was greatly increased in mice fed rye brans. Furthermore, bioprocessing of rye bran appears to serve as a beneficial way to improve the bioavailability of various phytochemicals.

Valérie Micard - One of the best experts on this subject based on the ideXlab platform.

  • Contribution of gut microbiota to metabolism of dietary glycine Betaine in mice and in vitro colonic fermentation
    Microbiome, 2019
    Co-Authors: Ville M. Koistinen, Valérie Micard, Natalia Rosa-sibakov, Olli Kärkkäinen, Jenna Jokkala, Klaudyna Borewicz, Iman Zarei, Seppo Auriola, Anna-marja Aura, Hauke Smidt
    Abstract:

    Background Accumulating evidence is supporting the protective effect of whole grains against several chronic diseases. Simultaneously, our knowledge is increasing on the impact of gut microbiota on our health and on how diet can modify the composition of our bacterial cohabitants. Herein, we studied C57BL/6 J mice fed with diets enriched with rye bran and wheat aleurone, conventional and germ-free C57BL/6NTac mice on a basal diet, and the colonic fermentation of rye bran in an in vitro model of the human gastrointestinal system. We performed 16S rRNA gene sequencing and metabolomics on the study samples to determine the effect of bran-enriched diets on the gut microbial composition and the potential contribution of microbiota to the metabolism of a novel group of betainized compounds. Results The bran-enriched study diets elevated the levels of betainized compounds in the colon contents of C57BL/6 J mice. The composition of microbiota changed, and the bran-enriched diets induced an increase in the relative abundance of several bacterial taxa, including Akkermansia , Bifidobacterium , Coriobacteriaceae , Lactobacillus , Parasutterella , and Ruminococcus , many of which are associated with improved health status or the metabolism of plant-based molecules. The levels of betainized compounds in the gut tissues of germ-free mice were significantly lower compared to conventional mice. In the in vitro model of the human gut, the production of betainized compounds was observed throughout the incubation, while the levels of glycine Betaine decreased. In cereal samples, only low levels or trace amounts of other Betaines than glycine Betaine were observed. Conclusions Our findings provide evidence that the bacterial taxa increased in relative abundance by the bran-based diet are also involved in the metabolism of glycine Betaine into other betainized compounds, adding another potential compound group acting as a mediator of the synergistic metabolic effect of diet and colonic microbiota.

  • Diets rich in whole grains increase betainized compounds associated with glucose metabolism
    American Journal of Clinical Nutrition, 2018
    Co-Authors: Olli Kärkkäinen, Natalia Rosa-sibakov, Marko Lehtonen, Maria A Lankinen, Marilena Vitale, Jenna Jokkala, Jukka Leppänen, Ville Koistinen, Rosalba Giacco, Valérie Micard
    Abstract:

    Background : Epidemiologic evidence suggests that diets rich in whole grains are associated with a reduced risk of developing chronic diseases and all-cause mortality. However, the molecular mechanisms behind these beneficial metabolic effects are poorly understood. Objective : Our aim was to investigate novel trimethylated (betainized) compounds from mice and humans, and their association with whole grain–rich diets and insulin resistance and insulin secretion. Design : Fasting plasma samples were obtained in a mouse (C57BL/6J male) feeding trial and a controlled dietary intervention. The mouse trial involved feeding the mice a rye and wheat bran–enriched feed which was compared with a high-fat diet. In the human trial, participants recruited from Kuopio, Finland (n = 69) and Naples, Italy (n = 54) with characteristics of the metabolic syndrome were randomly assigned to either a whole grain–enriched diet or a control diet for 12 wk. Plasma concentrations of betainized compounds were analyzed with the use of liquid chromatography-tandem mass spectrometry. Insulin resistance and insulin secretion were assessed in an oral-glucose-tolerance test and a meal-glucose-tolerance test. Results : The Betaines that were increased in mouse plasma after bran-enriched feeding were identified de novo via chemical synthesis and liquid chromatography-tandem mass spectrometry, and confirmed to be associated with an increased intake of whole-grain products in humans. In particular, the concentrations of pipecolic acid Betaine were increased at the end of the whole-grain intervention in both the Kuopio cohort (P < 0.001) and the Naples cohort (P < 0.05), and these concentrations inversely correlated with the postprandial glucose concentration. Furthermore, the concentration of valine Betaine was substantially increased during the intervention in Naples (P < 0.001) with an inverse correlation with the postprandial insulin concentration. In addition, the concentrations of other Betaines, e.g., glycine Betaine and proline Betaine, correlated with glucose and insulin concentrations at the end of the intervention. Conclusions : Novel betainized compounds in humans are associated with diets rich in whole grains, and they improve insulin resistance and insulin secretion. These results suggest that these novel compounds may contribute to the beneficial effects of whole grain–rich diets.

  • Amino acid-derived Betaines dominate as urinary markers for rye bran intake in mice fed high-fat diet--A nontargeted metabolomics study.
    Molecular nutrition & food research, 2015
    Co-Authors: Jenna Pekkinen, Hannu Mykkänen, Pekka Keski-rahkonen, Kaisa Poutanen, Valérie Micard, Natalia Rosa-sibakov, Marko Lehtonen, Kati Hanhineva
    Abstract:

    Scope Bioprocessing of whole grain cereals may affect the bioavailability of phytochemicals associated with grain fiber and ultimately lead to different health outcomes. Here, we studied the impact of long-term feeding with intact and bioprocessed rye bran on the urinary phytochemical profile of mice. Methods and results Nontargeted hydrophilic interaction chromatography-ESI-qTOF-MS metabolite profiling approach was applied on urine samples collected from three groups of diet-induced obese mice fed for 8 weeks with one of the three diets: high-fat (HF) control diet, HF diet enriched with intact rye bran, or HF diet enriched with bioprocessed rye bran. The most striking finding was the increased urinary excretion of several amino-acid derived Betaines after both rye diets. These included proline Betaine, alanine Betaine, valine Betaine, phenylalanine Betaine, pipecolic acid Betaine, and trigonelline, but not glycine Betaine. Furthermore, bioprocessing may have improved the bioavailability of rye-derived phytochemicals, as higher increase in, e.g. ferulic acid and benzoxazinoid metabolites were observed in urine of mice fed with bioprocessed than intact rye bran. Conclusion Urinary excretion of various Betaines was greatly increased in mice fed rye brans. Furthermore, bioprocessing of rye bran appears to serve as a beneficial way to improve the bioavailability of various phytochemicals.

  • Amino acid-derived Betaines dominate as urinary markers for rye bran intake in mice fed high-fat diet – a non-targeted metabolomics study
    Molecular Nutrition and Food Research, 2015
    Co-Authors: Jenna Pekkinen, Hannu Mykkänen, Pekka Keski-rahkonen, Kaisa Poutanen, Valérie Micard, Marko Lehtonen, Natalia Rosa, Kati Hanhineva
    Abstract:

    Scope : Bioprocessing of whole grain cereals may affect the bioavailability of phytochemicals associated with grain fiber and ultimately lead to different health outcomes. Here, we studied the impact of long-term feeding with intact and bioprocessed rye bran on the urinary phytochemical profile of mice. Methods and results : Non-targeted HILIC-ESI-qTOF-MS metabolite profiling approach was applied on urine samples collected from 3 groups of diet-induced obese mice fed for 8 weeks with one of the three diets: high-fat (HF) control diet, HF diet enriched with intact rye bran, or HF diet enriched with bioprocessed rye bran. The most striking finding was the increased urinary excretion of several amino-acid derived Betaines after both rye diets. These included proline Betaine, alanine Betaine, valine Betaine, phenylalanine Betaine, pipecolic acid Betaine, and trigonelline, but not glycine Betaine. Furthermore, bioprocessing may have improved the bioavailability of rye derived phytochemicals, as higher increase in e.g. ferulic acid and benzoxazinoid metabolites were observed in urine of mice fed with bioprocessed than intact rye bran. Conclusion : Urinary excretion of various Betaines was greatly increased in mice fed rye brans. Furthermore, bioprocessing of rye bran appears to serve as a beneficial way to improve the bioavailability of various phytochemicals.

Marko Lehtonen - One of the best experts on this subject based on the ideXlab platform.

  • Diets rich in whole grains increase betainized compounds associated with glucose metabolism
    American Journal of Clinical Nutrition, 2018
    Co-Authors: Olli Kärkkäinen, Natalia Rosa-sibakov, Marko Lehtonen, Maria A Lankinen, Marilena Vitale, Jenna Jokkala, Jukka Leppänen, Ville Koistinen, Rosalba Giacco, Valérie Micard
    Abstract:

    Background : Epidemiologic evidence suggests that diets rich in whole grains are associated with a reduced risk of developing chronic diseases and all-cause mortality. However, the molecular mechanisms behind these beneficial metabolic effects are poorly understood. Objective : Our aim was to investigate novel trimethylated (betainized) compounds from mice and humans, and their association with whole grain–rich diets and insulin resistance and insulin secretion. Design : Fasting plasma samples were obtained in a mouse (C57BL/6J male) feeding trial and a controlled dietary intervention. The mouse trial involved feeding the mice a rye and wheat bran–enriched feed which was compared with a high-fat diet. In the human trial, participants recruited from Kuopio, Finland (n = 69) and Naples, Italy (n = 54) with characteristics of the metabolic syndrome were randomly assigned to either a whole grain–enriched diet or a control diet for 12 wk. Plasma concentrations of betainized compounds were analyzed with the use of liquid chromatography-tandem mass spectrometry. Insulin resistance and insulin secretion were assessed in an oral-glucose-tolerance test and a meal-glucose-tolerance test. Results : The Betaines that were increased in mouse plasma after bran-enriched feeding were identified de novo via chemical synthesis and liquid chromatography-tandem mass spectrometry, and confirmed to be associated with an increased intake of whole-grain products in humans. In particular, the concentrations of pipecolic acid Betaine were increased at the end of the whole-grain intervention in both the Kuopio cohort (P < 0.001) and the Naples cohort (P < 0.05), and these concentrations inversely correlated with the postprandial glucose concentration. Furthermore, the concentration of valine Betaine was substantially increased during the intervention in Naples (P < 0.001) with an inverse correlation with the postprandial insulin concentration. In addition, the concentrations of other Betaines, e.g., glycine Betaine and proline Betaine, correlated with glucose and insulin concentrations at the end of the intervention. Conclusions : Novel betainized compounds in humans are associated with diets rich in whole grains, and they improve insulin resistance and insulin secretion. These results suggest that these novel compounds may contribute to the beneficial effects of whole grain–rich diets.

  • Amino acid-derived Betaines dominate as urinary markers for rye bran intake in mice fed high-fat diet--A nontargeted metabolomics study.
    Molecular nutrition & food research, 2015
    Co-Authors: Jenna Pekkinen, Hannu Mykkänen, Pekka Keski-rahkonen, Kaisa Poutanen, Valérie Micard, Natalia Rosa-sibakov, Marko Lehtonen, Kati Hanhineva
    Abstract:

    Scope Bioprocessing of whole grain cereals may affect the bioavailability of phytochemicals associated with grain fiber and ultimately lead to different health outcomes. Here, we studied the impact of long-term feeding with intact and bioprocessed rye bran on the urinary phytochemical profile of mice. Methods and results Nontargeted hydrophilic interaction chromatography-ESI-qTOF-MS metabolite profiling approach was applied on urine samples collected from three groups of diet-induced obese mice fed for 8 weeks with one of the three diets: high-fat (HF) control diet, HF diet enriched with intact rye bran, or HF diet enriched with bioprocessed rye bran. The most striking finding was the increased urinary excretion of several amino-acid derived Betaines after both rye diets. These included proline Betaine, alanine Betaine, valine Betaine, phenylalanine Betaine, pipecolic acid Betaine, and trigonelline, but not glycine Betaine. Furthermore, bioprocessing may have improved the bioavailability of rye-derived phytochemicals, as higher increase in, e.g. ferulic acid and benzoxazinoid metabolites were observed in urine of mice fed with bioprocessed than intact rye bran. Conclusion Urinary excretion of various Betaines was greatly increased in mice fed rye brans. Furthermore, bioprocessing of rye bran appears to serve as a beneficial way to improve the bioavailability of various phytochemicals.

  • Amino acid-derived Betaines dominate as urinary markers for rye bran intake in mice fed high-fat diet – a non-targeted metabolomics study
    Molecular Nutrition and Food Research, 2015
    Co-Authors: Jenna Pekkinen, Hannu Mykkänen, Pekka Keski-rahkonen, Kaisa Poutanen, Valérie Micard, Marko Lehtonen, Natalia Rosa, Kati Hanhineva
    Abstract:

    Scope : Bioprocessing of whole grain cereals may affect the bioavailability of phytochemicals associated with grain fiber and ultimately lead to different health outcomes. Here, we studied the impact of long-term feeding with intact and bioprocessed rye bran on the urinary phytochemical profile of mice. Methods and results : Non-targeted HILIC-ESI-qTOF-MS metabolite profiling approach was applied on urine samples collected from 3 groups of diet-induced obese mice fed for 8 weeks with one of the three diets: high-fat (HF) control diet, HF diet enriched with intact rye bran, or HF diet enriched with bioprocessed rye bran. The most striking finding was the increased urinary excretion of several amino-acid derived Betaines after both rye diets. These included proline Betaine, alanine Betaine, valine Betaine, phenylalanine Betaine, pipecolic acid Betaine, and trigonelline, but not glycine Betaine. Furthermore, bioprocessing may have improved the bioavailability of rye derived phytochemicals, as higher increase in e.g. ferulic acid and benzoxazinoid metabolites were observed in urine of mice fed with bioprocessed than intact rye bran. Conclusion : Urinary excretion of various Betaines was greatly increased in mice fed rye brans. Furthermore, bioprocessing of rye bran appears to serve as a beneficial way to improve the bioavailability of various phytochemicals.

Jenna Pekkinen - One of the best experts on this subject based on the ideXlab platform.

  • Amino acid-derived Betaines dominate as urinary markers for rye bran intake in mice fed high-fat diet--A nontargeted metabolomics study.
    Molecular nutrition & food research, 2015
    Co-Authors: Jenna Pekkinen, Hannu Mykkänen, Pekka Keski-rahkonen, Kaisa Poutanen, Valérie Micard, Natalia Rosa-sibakov, Marko Lehtonen, Kati Hanhineva
    Abstract:

    Scope Bioprocessing of whole grain cereals may affect the bioavailability of phytochemicals associated with grain fiber and ultimately lead to different health outcomes. Here, we studied the impact of long-term feeding with intact and bioprocessed rye bran on the urinary phytochemical profile of mice. Methods and results Nontargeted hydrophilic interaction chromatography-ESI-qTOF-MS metabolite profiling approach was applied on urine samples collected from three groups of diet-induced obese mice fed for 8 weeks with one of the three diets: high-fat (HF) control diet, HF diet enriched with intact rye bran, or HF diet enriched with bioprocessed rye bran. The most striking finding was the increased urinary excretion of several amino-acid derived Betaines after both rye diets. These included proline Betaine, alanine Betaine, valine Betaine, phenylalanine Betaine, pipecolic acid Betaine, and trigonelline, but not glycine Betaine. Furthermore, bioprocessing may have improved the bioavailability of rye-derived phytochemicals, as higher increase in, e.g. ferulic acid and benzoxazinoid metabolites were observed in urine of mice fed with bioprocessed than intact rye bran. Conclusion Urinary excretion of various Betaines was greatly increased in mice fed rye brans. Furthermore, bioprocessing of rye bran appears to serve as a beneficial way to improve the bioavailability of various phytochemicals.

  • Amino acid-derived Betaines dominate as urinary markers for rye bran intake in mice fed high-fat diet – a non-targeted metabolomics study
    Molecular Nutrition and Food Research, 2015
    Co-Authors: Jenna Pekkinen, Hannu Mykkänen, Pekka Keski-rahkonen, Kaisa Poutanen, Valérie Micard, Marko Lehtonen, Natalia Rosa, Kati Hanhineva
    Abstract:

    Scope : Bioprocessing of whole grain cereals may affect the bioavailability of phytochemicals associated with grain fiber and ultimately lead to different health outcomes. Here, we studied the impact of long-term feeding with intact and bioprocessed rye bran on the urinary phytochemical profile of mice. Methods and results : Non-targeted HILIC-ESI-qTOF-MS metabolite profiling approach was applied on urine samples collected from 3 groups of diet-induced obese mice fed for 8 weeks with one of the three diets: high-fat (HF) control diet, HF diet enriched with intact rye bran, or HF diet enriched with bioprocessed rye bran. The most striking finding was the increased urinary excretion of several amino-acid derived Betaines after both rye diets. These included proline Betaine, alanine Betaine, valine Betaine, phenylalanine Betaine, pipecolic acid Betaine, and trigonelline, but not glycine Betaine. Furthermore, bioprocessing may have improved the bioavailability of rye derived phytochemicals, as higher increase in e.g. ferulic acid and benzoxazinoid metabolites were observed in urine of mice fed with bioprocessed than intact rye bran. Conclusion : Urinary excretion of various Betaines was greatly increased in mice fed rye brans. Furthermore, bioprocessing of rye bran appears to serve as a beneficial way to improve the bioavailability of various phytochemicals.