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Chien Chih Chen - One of the best experts on this subject based on the ideXlab platform.
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Moscatilin Inhibits Metastatic Behavior of Human Hepatocellular Carcinoma Cells: A Crucial Role of uPA Suppression via Akt/NF-κB-Dependent Pathway.
International journal of molecular sciences, 2021Co-Authors: Meng-shih Weng, Chien Chih Chen, Wei-cheng Chen, Kai-ting Chien, Chih-wen Chi, Ching-hu Chung, Chia-wen Huang, Po-chuan Wang, An Chi TsaiAbstract:Hepatocellular carcinoma (HCC) frequently shows early invasion into blood vessels as well as intrahepatic metastasis. Innovations of novel small-molecule agents to block HCC invasion and subsequent metastasis are urgently needed. Moscatilin is a Bibenzyl Derivative extracted from the stems of a traditional Chinese medicine, orchid Dendrobium loddigesii. Although moscatilin has been reported to suppress tumor angiogenesis and growth, the anti-metastatic property of moscatilin has not been elucidated. The present results revealed that moscatilin inhibited metastatic behavior of HCC cells without cytotoxic fashion in highly invasive human HCC cell lines. Furthermore, moscatilin significantly suppressed the activity of urokinase plasminogen activator (uPA), but not matrix metalloproteinase (MMP)-2 and MMP-9. Interestingly, moscatilin-suppressed uPA activity was through down-regulation the protein level of uPA, and did not impair the uPA receptor and uPA inhibitory molecule (PAI-1) expressions. Meanwhile, the mRNA expression of uPA was inhibited via moscatilin in a concentration-dependent manner. In addition, the expression of phosphorylated Akt, rather than ERK1/2, was inhibited by moscatilin treatment. The expression of phosphor-IκBα, and -p65, as well as κB-luciferase activity were also repressed after moscatilin treatment. Transfection of constitutively active Akt (Myr-Akt) obviously restored the moscatilin-inhibited the activation of NF-κB and uPA, and cancer invasion in HCC cells. Taken together, these results suggest that moscatilin impedes HCC invasion and uPA expression through the Akt/NF-κB signaling pathway. Moscatilin might serve as a potential anti-metastatic agent against the disease progression of human HCC.
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moscatilin induces apoptosis and mitotic catastrophe in human esophageal cancer cells
Journal of Medicinal Food, 2013Co-Authors: Chien-an Chen, Chien-chang Shen, Hen-hong Chang, Chien Chih Chen, Yu-jen ChenAbstract:Moscatilin, a Bibenzyl Derivative from the orchid Dendrobium loddigesii, has been shown to possess anticancer activity. We examined the effect of moscatilin on human esophageal cancer cells, including squamous cell carcinoma (SCC) and adenocarcinoma (ADC) cells and its possible mechanisms. Moscatilin suppressed the growth of both the histological cell lines in a dose- and time-dependent manner. Morphological changes indicative of apoptosis and mitotic catastrophe were observed following moscatilin treatment. The population of cells in the sub-G1 phase and polyploidy phase significantly increased after treatment. Immunofluorescence revealed multipolar mitosis and subsequent multinucleation in moscatilin-treated cells, indicating the development of mitotic catastrophe. Western blot showed a marked increase in expressions of polo-like kinase 1 and cyclin B1 after exposure to moscatilin. In conclusion, moscatilin inhibits growth and induces apoptosis and mitotic catastrophe in human esophageal SCC- and ADC-derived cell lines, indicating that moscatilin has broad potential against esophageal cancer.
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Moscatilin induces apoptosis and mitotic catastrophe in human esophageal cancer cells.
Journal of Medicinal Food, 2013Co-Authors: Chien-an Chen, Chien-chang Shen, Hen-hong Chang, Chien Chih Chen, Yu-jen ChenAbstract:Abstract Moscatilin, a Bibenzyl Derivative from the orchid Dendrobium loddigesii, has been shown to possess anticancer activity. We examined the effect of moscatilin on human esophageal cancer cells, including squamous cell carcinoma (SCC) and adenocarcinoma (ADC) cells and its possible mechanisms. Moscatilin suppressed the growth of both the histological cell lines in a dose- and time-dependent manner. Morphological changes indicative of apoptosis and mitotic catastrophe were observed following moscatilin treatment. The population of cells in the sub-G1 phase and polyploidy phase significantly increased after treatment. Immunofluorescence revealed multipolar mitosis and subsequent multinucleation in moscatilin-treated cells, indicating the development of mitotic catastrophe. Western blot showed a marked increase in expressions of polo-like kinase 1 and cyclin B1 after exposure to moscatilin. In conclusion, moscatilin inhibits growth and induces apoptosis and mitotic catastrophe in human esophageal SCC- a...
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Abstract 4236: Moscatilin inhibits invasion by suppressing urokinase plasminogen activator expression through Akt inactivation in human hepatocellular carcinoma cells
Cancer Chemistry, 2011Co-Authors: Shih-wei Wang, Chien Chih Chen, An Chi Tsai, Shiow Lin Pan, Jih-hwa Guh, Chin-sung Chien, Che-ming TengAbstract:Proceedings: AACR 102nd Annual Meeting 2011‐‐ Apr 2‐6, 2011; Orlando, FL Purpose: Hepatocellular carcinoma (HCC) frequently shows early invasion into blood vessels as well as intrahepatic metastasis. The innovations of novel small-molecule agents to block HCC invasion and subsequent metastasis are urgently needed. Moscatilin is a Bibenzyl Derivative extracted from the stems of a traditional Chinese medicine, Orchid Dendrobrium loddigesii. Previous studies demonstrated that moscatilin exerted potent cytotoxic effects against numerous lines of cancer cells. Recently, moscatilin has been reported to suppress tumor angiogenesis. However, the anti-metastatic property of moscatilin has not been elucidated. Methods: Multiple molecular and pharmacological approaches such as invasion assay, MTT assay, ELISA assay, zymography, real-time PCR, Western blot, luciferase reporter gene assay, and chorioallantoic membrane (CAM) intravasation assay were used to determine the anti-metastatic effects of moscatilin in SK-Hep-1 cells, a highly metastatic human HCC cell line. Results: We found moscatilin inhibited cell invasion in a concentration-dependent manner in SK-Hep-1 cells in the absence of cytotoxicity. Moscatilin did not affect proteolytic activities of matrix metalloproteinase (MMP) -2 and MMP-9, but profoundly suppressed urokinase plasminogen activator (uPA) activity. We next found that moscatilin substantially inhibited protein expression of uPA but did not obviously impair uPAR and PAI-1 expression. To examine the upstream signaling of uPA suppression, the ERK1/2, Akt, mTOR, and NF-κB signaling pathways were evaluated in moscatilin-treated cells. We demonstrated that moscatilin dramatically inhibited Akt phosphorylation, but not ERK1/2 phosphorylation. Moscatilin did not reduce the phosphorylation of mTOR as well as of components of the translational machinery including p70S6K, 4E-BP1 and eIF4E. Additionally, IKKα/β phosphorylation, IκBα phosphorylation, p65 phosphorylation at Ser536, and κB-luciferase activity were not inhibited by moscatilin. However, Moscatilin repressed the expression of uPA mRNA, suggesting that moscatilin regulated the uPA production in transcriptional level. Transfection of constitutively active Akt (Myr-Akt) partly restored the moscatilin induced Akt inactivation. The inhibition of cell invasion, uPA activity and gene expression in response to moscatilin were also attenuated. Moreover, we found that moscatilin significantly inhibited cell metastasis using CAM intravasation assay, a well established in vivo model for metastasis. Conclusions: This study provides evidence that moscatilin induces Akt inactivation and uPA suppression, which leads to inhibition of invasion and metastasis in human HCC cells. Our results suggest that moscatilin is a novel anti-metastatic agent, which has great potential for further development for treating cancer. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 4236. doi:10.1158/1538-7445.AM2011-4236
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Moscatilin, a Bibenzyl Derivative from the India orchid Dendrobrium loddigesii, suppresses tumor angiogenesis and growth in vitro and in vivo
Cancer letters, 2009Co-Authors: An Chi Tsai, Chien-chang Shen, Chien Chih Chen, Shiow Lin Pan, Cho Hwa Liao, Jih-hwa Guh, Shih-wei Wang, Hui Lung Sun, Yi Nan Liu, Ya Ling ChangAbstract:Attacking angiogenesis is considered an effective strategy for controls the expansion and metastasis of tumors and other related-diseases. The aim of this study was to assess the effects of moscatilin, a Bibenzyl Derivative, on VEGF and bFGF-induced angiogenesis in cultured human umbilical vein endothelial cells (HUVECs) in vitro and in vivo. Moscatilin significantly inhibited growth of lung cancer cell line A549 (NSCLC) and suppressed growth factor-induced neovascularization. In addition, VEGF- and bFGF-induced cell proliferation, migration, and tube formation of HUVECs was markedly inhibited by moscatilin. Western blotting analysis of cell signaling molecules indicated that moscatilin inhibited ERK1/2, Akt, and eNOS signaling pathways in HUVECs. These results suggest that inhibition of angiogenesis by moscatilin may be a major mechanism in cancer therapy.
Yoshinori Asakawa - One of the best experts on this subject based on the ideXlab platform.
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Novel prenyl Bibenzyls from the New Zealand liverwort Marsupidium epiphytum.
Chemical & pharmaceutical bulletin, 2011Co-Authors: Masao Toyota, Ikuko Omatsu, John E. Braggins, Yoshinori AsakawaAbstract:The ether extract of the New Zealand liverwort Marsupidium epiphytum gave four new prenyl Bibenzyl Derivatives, along with a known prenyl Bibenzyl Derivative which has been isolated from the Ecuadorian liverwort Lethocolea glossophylla; their structures were determined by 2D-NMR spectrum. The chemical constituents of Marsupidium epiphytum are highly characteristic since they elaborate dihydrooxepin type compounds and prenyl type Bibenzyls. These structures are closely related to those found in Radula spp. (Radulaceae), although Bibenzyls with two prenyl groups have not been isolated from the Radula spp. Although Marsupidium spp. are different from Radula spp. morphologically, the constituents are closely related. This is the first example of isolation of prenyl Bibenzyl Derivatives from M. epiphytum, a species which has not previously been investigated phytochemically.
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Hymenosides A-F, six new hemiterpene glucosides from the Japanese fern Hymenophyllum barbatum.
CHEMICAL & PHARMACEUTICAL BULLETIN, 2001Co-Authors: Yasushi Oiso, Masao Toyota, Yoshinori AsakawaAbstract:In the course of investigation of the bitter-tasting substances of the Japanese fern Hymenophyllum barbatum belonging to the family Hymenophyllaceae, six new hemiterpene glucosides called hymenosides A-F (1-6) have been isolated from the methanol extract, together with an acyclic bis-Bibenzyl Derivative, perrottetin H. This paper deals with the structure elucidation of the newly isolated glucosides.
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occurrence of a bis Bibenzyl Derivative in the japanese fern hymenophyllum barbatum first isolation and identification of perrottetin h from the pteridophytes
Chemical & Pharmaceutical Bulletin, 1999Co-Authors: Yasushi Oiso, Masao Toyota, Yoshinori AsakawaAbstract:The methanol extract of the Japanese fern Hymenophyllum barbatum was chromatographed on Sephadex LH-20 and HPLC to give perrottetin H, an acyclic bis-Bibenzyl Derivative which has been isolated from the liverwort Jubula japonica. This is the first isolation and identification of bis-Bibenzyl from the pteridophytes. The occurrence of bis-Bibenzyl in both pteridophytes and liverwort is very important in determining the evolutionary ladder of both terrestrial spore-forming green plants.
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Terpenoids from the Japanese liverworts Jackiella javanica and Jungermannia infusca
Phytochemistry, 1997Co-Authors: Fumihiro Nagashima, Akiko Tamada, Noriko Fujii, Yoshinori AsakawaAbstract:Abstract Five ent -verticillane-type diterpenoids have been isolated from the Japanese liverwort Jackiella javanica , along with previously known sesqui- and di-terpenoids. The chemical shifts of the 1 H and 13 C NMR spectra of ent -verticillane-type diterpenoids are given. A new monocyclic diterpenoid, infuscatrienol, three known sesqui- and di-terpenoids, and a bis -Bibenzyl Derivative have been isolated from the Japanese Jungermannia infusca .
An Chi Tsai - One of the best experts on this subject based on the ideXlab platform.
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Moscatilin Inhibits Metastatic Behavior of Human Hepatocellular Carcinoma Cells: A Crucial Role of uPA Suppression via Akt/NF-κB-Dependent Pathway.
International journal of molecular sciences, 2021Co-Authors: Meng-shih Weng, Chien Chih Chen, Wei-cheng Chen, Kai-ting Chien, Chih-wen Chi, Ching-hu Chung, Chia-wen Huang, Po-chuan Wang, An Chi TsaiAbstract:Hepatocellular carcinoma (HCC) frequently shows early invasion into blood vessels as well as intrahepatic metastasis. Innovations of novel small-molecule agents to block HCC invasion and subsequent metastasis are urgently needed. Moscatilin is a Bibenzyl Derivative extracted from the stems of a traditional Chinese medicine, orchid Dendrobium loddigesii. Although moscatilin has been reported to suppress tumor angiogenesis and growth, the anti-metastatic property of moscatilin has not been elucidated. The present results revealed that moscatilin inhibited metastatic behavior of HCC cells without cytotoxic fashion in highly invasive human HCC cell lines. Furthermore, moscatilin significantly suppressed the activity of urokinase plasminogen activator (uPA), but not matrix metalloproteinase (MMP)-2 and MMP-9. Interestingly, moscatilin-suppressed uPA activity was through down-regulation the protein level of uPA, and did not impair the uPA receptor and uPA inhibitory molecule (PAI-1) expressions. Meanwhile, the mRNA expression of uPA was inhibited via moscatilin in a concentration-dependent manner. In addition, the expression of phosphorylated Akt, rather than ERK1/2, was inhibited by moscatilin treatment. The expression of phosphor-IκBα, and -p65, as well as κB-luciferase activity were also repressed after moscatilin treatment. Transfection of constitutively active Akt (Myr-Akt) obviously restored the moscatilin-inhibited the activation of NF-κB and uPA, and cancer invasion in HCC cells. Taken together, these results suggest that moscatilin impedes HCC invasion and uPA expression through the Akt/NF-κB signaling pathway. Moscatilin might serve as a potential anti-metastatic agent against the disease progression of human HCC.
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Abstract 4236: Moscatilin inhibits invasion by suppressing urokinase plasminogen activator expression through Akt inactivation in human hepatocellular carcinoma cells
Cancer Chemistry, 2011Co-Authors: Shih-wei Wang, Chien Chih Chen, An Chi Tsai, Shiow Lin Pan, Jih-hwa Guh, Chin-sung Chien, Che-ming TengAbstract:Proceedings: AACR 102nd Annual Meeting 2011‐‐ Apr 2‐6, 2011; Orlando, FL Purpose: Hepatocellular carcinoma (HCC) frequently shows early invasion into blood vessels as well as intrahepatic metastasis. The innovations of novel small-molecule agents to block HCC invasion and subsequent metastasis are urgently needed. Moscatilin is a Bibenzyl Derivative extracted from the stems of a traditional Chinese medicine, Orchid Dendrobrium loddigesii. Previous studies demonstrated that moscatilin exerted potent cytotoxic effects against numerous lines of cancer cells. Recently, moscatilin has been reported to suppress tumor angiogenesis. However, the anti-metastatic property of moscatilin has not been elucidated. Methods: Multiple molecular and pharmacological approaches such as invasion assay, MTT assay, ELISA assay, zymography, real-time PCR, Western blot, luciferase reporter gene assay, and chorioallantoic membrane (CAM) intravasation assay were used to determine the anti-metastatic effects of moscatilin in SK-Hep-1 cells, a highly metastatic human HCC cell line. Results: We found moscatilin inhibited cell invasion in a concentration-dependent manner in SK-Hep-1 cells in the absence of cytotoxicity. Moscatilin did not affect proteolytic activities of matrix metalloproteinase (MMP) -2 and MMP-9, but profoundly suppressed urokinase plasminogen activator (uPA) activity. We next found that moscatilin substantially inhibited protein expression of uPA but did not obviously impair uPAR and PAI-1 expression. To examine the upstream signaling of uPA suppression, the ERK1/2, Akt, mTOR, and NF-κB signaling pathways were evaluated in moscatilin-treated cells. We demonstrated that moscatilin dramatically inhibited Akt phosphorylation, but not ERK1/2 phosphorylation. Moscatilin did not reduce the phosphorylation of mTOR as well as of components of the translational machinery including p70S6K, 4E-BP1 and eIF4E. Additionally, IKKα/β phosphorylation, IκBα phosphorylation, p65 phosphorylation at Ser536, and κB-luciferase activity were not inhibited by moscatilin. However, Moscatilin repressed the expression of uPA mRNA, suggesting that moscatilin regulated the uPA production in transcriptional level. Transfection of constitutively active Akt (Myr-Akt) partly restored the moscatilin induced Akt inactivation. The inhibition of cell invasion, uPA activity and gene expression in response to moscatilin were also attenuated. Moreover, we found that moscatilin significantly inhibited cell metastasis using CAM intravasation assay, a well established in vivo model for metastasis. Conclusions: This study provides evidence that moscatilin induces Akt inactivation and uPA suppression, which leads to inhibition of invasion and metastasis in human HCC cells. Our results suggest that moscatilin is a novel anti-metastatic agent, which has great potential for further development for treating cancer. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 4236. doi:10.1158/1538-7445.AM2011-4236
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Moscatilin, a Bibenzyl Derivative from the India orchid Dendrobrium loddigesii, suppresses tumor angiogenesis and growth in vitro and in vivo
Cancer letters, 2009Co-Authors: An Chi Tsai, Chien-chang Shen, Chien Chih Chen, Shiow Lin Pan, Cho Hwa Liao, Jih-hwa Guh, Shih-wei Wang, Hui Lung Sun, Yi Nan Liu, Ya Ling ChangAbstract:Attacking angiogenesis is considered an effective strategy for controls the expansion and metastasis of tumors and other related-diseases. The aim of this study was to assess the effects of moscatilin, a Bibenzyl Derivative, on VEGF and bFGF-induced angiogenesis in cultured human umbilical vein endothelial cells (HUVECs) in vitro and in vivo. Moscatilin significantly inhibited growth of lung cancer cell line A549 (NSCLC) and suppressed growth factor-induced neovascularization. In addition, VEGF- and bFGF-induced cell proliferation, migration, and tube formation of HUVECs was markedly inhibited by moscatilin. Western blotting analysis of cell signaling molecules indicated that moscatilin inhibited ERK1/2, Akt, and eNOS signaling pathways in HUVECs. These results suggest that inhibition of angiogenesis by moscatilin may be a major mechanism in cancer therapy.
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Moscatilin, a novel tumor vascular-targeting agent, induced cell cycle arrest and apoptosis in human umbilical vein endothelial cells
Clinical Cancer Research, 2007Co-Authors: An Chi Tsai, Chien Chih Chen, Shiow Lin Pan, Cho Hwa Liao, Jih-hwa Guh, Shih-wei Wang, Hui Lung Sun, Yi Nan Liu, Che-ming TengAbstract:B44 Selective disrupting the vascular network of tumors represented a critical approach to cancer treatment. Moscatilin, a Bibenzyl Derivative originally purified from the India orchid Dendrobrium loddigesii , has previously been reported to exert potent cytotoxic effect against human tumor cells. In this study, we investigated the apoptotic activity of moscatilin in cultured human umbilical vein endothelial cell (HUVEC) by means of crystal violet, MTT assay, DAPI staining, and flow cytometry. Mosactilin was found to inhibit the growth of cultured proliferating HUVECs in a time- and concentration-dependent manner . Cell cycle analysis showed G 2 /M phase arrest, and apoptosis in HUVECs following 24 h exposure to moscatilin. We performed both immunofluorescence staining of tubulin and in vitro tubulin assembly assay to show that moscatilin can inhibit microtubule assembly. Exposure of HUVECs to moscatilin showed an increase in the expression of MPM2, a marker of mitosis. Analysis of the cell cycle regulatory proteins demonstrated that moscatilin up-regulated the protein levels of cyclin B1, p53, p21 and down-regulated cdc25C, but did not change the steady-level of Cdc2. In addition, moscatilin also activated caspase-3, -7, -9 and proteolytic cleavage of poly (ADP-ribose) polymerase, and increased that of growth arrest/death signals such as Bcl-2 and Bax. Finally, moscatilin significant inhibited VEGF- and bFGF-induced neovascularization using the mouse Matrigel implant model . Results of in vivo tumor growth assay indicated that moscatilin inhibited human lung adenocarcinoma epithelial cell line A549 growth and induces vascular shutdown within tumor. Base on these results, the ability of moscatilin to selectively induce apoptosis and growth arrest in endothelial cells potentially hints at vascular targeting to contribute to anticancer activity.
Boonchoo Sritularak - One of the best experts on this subject based on the ideXlab platform.
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Immune modulatory effect of a novel 4,5-dihydroxy-3,3´,4´-trimethoxyBibenzyl from Dendrobium lindleyi.
PloS one, 2020Co-Authors: Pichayatri Khoonrit, Alp Mirdogan, Adeline Dehlinger, Wanwimon Mekboonsonglarp, Kittisak Likhitwitayawuid, Josef Priller, Chotima Böttcher, Boonchoo SritularakAbstract:Dendrobium Bibenzyls and phenanthrenes such as chrysotoxine, cypripedin, gigantol and moscatilin have been reported to show promising inhibitory effects on lung cancer growth and metastasis in ex vivo human cell line models, suggesting their potential for clinical application in patients with lung cancer. However, it remains to be determined whether these therapeutic effects can be also seen in primary human cells and/or in vivo. In this study, we comparatively investigated the immune modulatory effects of Bibenzyls and phenanthrenes, including a novel Dendrobium Bibenzyl Derivative, in primary human monocytes. All compounds were isolated and purified from a Thai orchid Dendrobium lindleyi Steud, a new source of therapeutic compounds with promising potential of tissue culture production. We detected increased frequencies of TNF- and IL-6-expressing monocytes after treatment with gigantol and cypripedin, whereas chrysotoxine and moscatilin did not alter the expression of these cytokines in monocytes. Interestingly, the new 4,5-dihydroxy-3,3',4'-trimethoxyBibenzyl Derivative showed dose-dependent immune modulatory effects in lipopolysaccharide (LPS)-treated CD14lo and CD14hi monocytes. Together, our findings show immune modulatory effects of the new Bibenzyl Derivative from Dendrobium lindleyi on different monocyte sub-populations. However, therapeutic consequences of these different monocyte populations on human diseases including cancer remain to be investigated.
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Moscatilin Inhibits Lung Cancer Cell Motility and Invasion via Suppression of Endogenous Reactive Oxygen Species
BioMed research international, 2013Co-Authors: Akkarawut Kowitdamrong, Boonchoo Sritularak, Pithi Chanvorachote, Varisa PongrakhananonAbstract:Lung cancer is the leading cause of death among cancer patients worldwide, and most of them have died from metastasis. Migration and invasion are prerequisite processes associated with high metastasis potential in cancers. Moscatilin, a Bibenzyl Derivative isolated from the Thai orchid Dendrobium pulchellum, has been shown to have anticancer effect against numerous cancer cell lines. However, little is known regarding the effect of moscatilin on cancer cell migration and invasion. The present study demonstrates that nontoxic concentrations of moscatilin were able to inhibit human nonsmall cell lung cancer H23 cell migration and invasion. The inhibitory effect of moscatilin was associated with an attenuation of endogenous reactive oxygen species (ROS), in which hydroxyl radical () was identified as a dominant species in the suppression of filopodia formation. Western blot analysis also revealed that moscatilin downregulated activated focal adhesion kinase (phosphorylated FAK, Tyr 397) and activated ATP-dependent tyrosine kinase (phosphorylated Akt, Ser 473), whereas their parental counterparts were not detectable changed. In conclusion, our results indicate the novel molecular basis of moscalitin-inhibiting lung cancer cell motility and invasion and demonstrate a promising antimetastatic potential of such an agent for lung cancer therapy.
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Research Article Moscatilin Inhibits Lung Cancer Cell Motility and Invasion via Suppression of Endogenous Reactive Oxygen Species
2013Co-Authors: Akkarawut Kowitdamrong, Boonchoo Sritularak, Pithi Chanvorachote, Varisa PongrakhananonAbstract:Copyright © 2013 Akkarawut Kowitdamrong et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Lung cancer is the leading cause of death among cancer patients worldwide, andmost of them have died frommetastasis. Migration and invasion are prerequisite processes associated with high metastasis potential in cancers. Moscatilin, a Bibenzyl Derivative isolated from theThai orchidDendrobium pulchellum, has been shown to have anticancer effect against numerous cancer cell lines. However, little is known regarding the effect of moscatilin on cancer cell migration and invasion. The present study demonstrates that nontoxic concentrations of moscatilin were able to inhibit human nonsmall cell lung cancer H23 cell migration and invasion. The inhibitory effect of moscatilin was associated with an attenuation of endogenous reactive oxygen species (ROS), in which hydroxyl radical (OH∙) was identified as a dominant species in the suppression of filopodia formation. Western blot analysis also revealed that moscatilin downregulated activated focal adhesion kinase (phosphorylated FAK, Tyr 397) and activated ATP-dependent tyrosine kinase (phosphorylated Akt, Ser 473), whereas their parental counterparts were not detectable changed. I
Chien-chang Shen - One of the best experts on this subject based on the ideXlab platform.
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moscatilin induces apoptosis and mitotic catastrophe in human esophageal cancer cells
Journal of Medicinal Food, 2013Co-Authors: Chien-an Chen, Chien-chang Shen, Hen-hong Chang, Chien Chih Chen, Yu-jen ChenAbstract:Moscatilin, a Bibenzyl Derivative from the orchid Dendrobium loddigesii, has been shown to possess anticancer activity. We examined the effect of moscatilin on human esophageal cancer cells, including squamous cell carcinoma (SCC) and adenocarcinoma (ADC) cells and its possible mechanisms. Moscatilin suppressed the growth of both the histological cell lines in a dose- and time-dependent manner. Morphological changes indicative of apoptosis and mitotic catastrophe were observed following moscatilin treatment. The population of cells in the sub-G1 phase and polyploidy phase significantly increased after treatment. Immunofluorescence revealed multipolar mitosis and subsequent multinucleation in moscatilin-treated cells, indicating the development of mitotic catastrophe. Western blot showed a marked increase in expressions of polo-like kinase 1 and cyclin B1 after exposure to moscatilin. In conclusion, moscatilin inhibits growth and induces apoptosis and mitotic catastrophe in human esophageal SCC- and ADC-derived cell lines, indicating that moscatilin has broad potential against esophageal cancer.
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Moscatilin induces apoptosis and mitotic catastrophe in human esophageal cancer cells.
Journal of Medicinal Food, 2013Co-Authors: Chien-an Chen, Chien-chang Shen, Hen-hong Chang, Chien Chih Chen, Yu-jen ChenAbstract:Abstract Moscatilin, a Bibenzyl Derivative from the orchid Dendrobium loddigesii, has been shown to possess anticancer activity. We examined the effect of moscatilin on human esophageal cancer cells, including squamous cell carcinoma (SCC) and adenocarcinoma (ADC) cells and its possible mechanisms. Moscatilin suppressed the growth of both the histological cell lines in a dose- and time-dependent manner. Morphological changes indicative of apoptosis and mitotic catastrophe were observed following moscatilin treatment. The population of cells in the sub-G1 phase and polyploidy phase significantly increased after treatment. Immunofluorescence revealed multipolar mitosis and subsequent multinucleation in moscatilin-treated cells, indicating the development of mitotic catastrophe. Western blot showed a marked increase in expressions of polo-like kinase 1 and cyclin B1 after exposure to moscatilin. In conclusion, moscatilin inhibits growth and induces apoptosis and mitotic catastrophe in human esophageal SCC- a...
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Moscatilin, a Bibenzyl Derivative from the India orchid Dendrobrium loddigesii, suppresses tumor angiogenesis and growth in vitro and in vivo
Cancer letters, 2009Co-Authors: An Chi Tsai, Chien-chang Shen, Chien Chih Chen, Shiow Lin Pan, Cho Hwa Liao, Jih-hwa Guh, Shih-wei Wang, Hui Lung Sun, Yi Nan Liu, Ya Ling ChangAbstract:Attacking angiogenesis is considered an effective strategy for controls the expansion and metastasis of tumors and other related-diseases. The aim of this study was to assess the effects of moscatilin, a Bibenzyl Derivative, on VEGF and bFGF-induced angiogenesis in cultured human umbilical vein endothelial cells (HUVECs) in vitro and in vivo. Moscatilin significantly inhibited growth of lung cancer cell line A549 (NSCLC) and suppressed growth factor-induced neovascularization. In addition, VEGF- and bFGF-induced cell proliferation, migration, and tube formation of HUVECs was markedly inhibited by moscatilin. Western blotting analysis of cell signaling molecules indicated that moscatilin inhibited ERK1/2, Akt, and eNOS signaling pathways in HUVECs. These results suggest that inhibition of angiogenesis by moscatilin may be a major mechanism in cancer therapy.