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Soković Marina - One of the best experts on this subject based on the ideXlab platform.

  • Griseofulvin Derivatives: Synthesis, Molecular Docking and Biological Evaluation.
    2019
    Co-Authors: Kartsev Victor, Geronikaki Athina, Petrou Anthi, Lichitsky Boris, Kostić Marina, Smiljković Marija, Soković Marina, Sirakanyan Samvel
    Abstract:

    BACKGROUND Griseofulvin - a mold metabolite produced by Penisilium griseofulvum is known as an antifungal drug. OBJECTIVE Thus, the goal of this paper is the design and synthesis of new griseofulvin derivatives and evaluation of their antifungal activity. METHODS Forty-two new compounds were synthesized using classical methods of organic synthesis and evaluated for their antimicrobial activity by microdilution method. RESULTS All forty-two new compounds exhibited very good activity against eight tested micromycetes with MIC ranging from 0.0075-0.055 mg/ml and MFC from 0.02-024 mg/ml. All compounds exhibited better activity than reference drugs ketoconazole (7-42 times) and Bifonazole (3-16 fold). The most promising was compound 15. The most sensitive fungal was found to be T. viride, while the most resistant, as was expected, was A. fumigatus. It should be mentioned that most of compounds exhibited better activity than griseofulvin. The molecular docking studies revealed that the most active compound have the same hydrophobic and H-bonding interactions with Thr276 residue observed for griseofulvin forming 3 hydrogen bonds while griseofulvin only one. In general, the molecular docking results coincide with experimental. CONCLUSION Forty-two giseofulvin derivatives were designed, synthesized and evaluated for antimicrobial activity. These derivatives revealed good antifungal activity, better than reference drugs ketoconazole, Bifonazole, and griseofulvin as well

  • Antimicrobial activity and chemical composition of Brunfelsia uniflora flower oleoresin extracted by supercritical carbon dioxide
    'Genetics and Molecular Research', 2017
    Co-Authors: Thiesen L. C. T., Soković Marina, Tešević Vele, Glamočlija Jasmina, Sugauara E. Y. Y., Goncalves J. E., Gazim Z. C., Linde G. A., Colauto N. B.
    Abstract:

    Brunfelsia genus is traditionally utilized in popular medicine due to its antibacterial and antifungal properties to name but a few. However, studies on the antimicrobial activity of Brunfelsia uniflora flower oleoresin have not been found yet. This study aimed to evaluate the chemical composition and antimicrobial activity of B. uniflora flower oleoresin obtained by supercritical carbon dioxide. Oleoresin from the plant dried flowers was obtained by carbon dioxide, and the chemical composition was analyzed by gas chromatographic-mass spectrometry. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and minimum fungicidal concentration (MFC) of this oleoresin for seven bacteria and eight fungi were determined using 96-well microtiter plates. The oleoresin MBC for Bacillus cereus, Enterobacter cloacae, Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella enterica, and Staphylococcus aureus ranged from 0.01 to 0.08 mg/mL, whereas the controls streptomycin and ampicillin varied from 0.1 and 0.5 mg/mL. The oleoresin MFC for Aspergillus fumigatus, Aspergillus niger, Aspergillus ochraceus, Aspergillus versicolor, Penicillium funiculosum, Penicillium ochrochloron, Penicillium verrucosum var. cyclopium, and Trichoderma viride varied from 0.01 to 0.08 mg/mL, whereas the controls Bifonazole and ketoconazole ranged from 0.2 to 3.5 mg/mL. The oleoresin obtained by supercritical carbon dioxide presented bacteriostatic, bactericidal, fungistatic, and fungicidal activities that were higher than the positive controls streptomycin, ampicillin, Bifonazole, and ketoconazole. The high antimicrobial activity was related to the high content of (E, E)-geranyllinalool that composes 21.0% of the oleoresin and a possible synergic action with fatty acid esters that made up 50.5% of the oleoresin. The oleoresin antimicrobial activity against common multiresistant bacteria in severe infectious processes as P. aeruginosa or against toxin-producing fungi such as P. ochrochloron or fungi that are difficult to control such as T. viride suggests the development of promising applications of this product in the food, farming, livestock, and pharmaceutical industry

  • Antimicrobial activity and chemical composition of Brunfelsia uniflora flower oleoresin extracted by supercritical carbon dioxide
    2017
    Co-Authors: Thiesen, Letícia De Cassia Tavares, Soković Marina, Tešević Vele, Glamočlija Jasmina, Linde, Giani Andrea, Sugauara, Elaine Yae Yamashita, Gonçalves, José Eduardo, Gazim, Zilda Cristiani, Colauto Nelson Barros
    Abstract:

    Brunfelsia genus is traditionally utilized in popular medicine due to its antibacterial and antifungal properties to name but a few. However, studies on the antimicrobial activity of Brunfelsia uniflora flower oleoresin have not been found yet. This study aimed to evaluate the chemical composition and antimicrobial activity of B. uniflora flower oleoresin obtained by supercritical carbon dioxide. Oleoresin from the plant dried flowers was obtained by carbon dioxide, and the chemical composition was analyzed by gas chromatographic-mass spectrometry. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and minimum fungicidal concentration (MFC) of this oleoresin for seven bacteria and eight fungi were determined using 96-well microtiter plates. The oleoresin MBC for Bacillus cereus, Enterobacter cloacae, Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella enterica, and Staphylococcus aureus ranged from 0.01 to 0.08 mg/mL, whereas the controls streptomycin and ampicillin varied from 0.1 and 0.5 mg/ mL. The oleoresin MFC for Aspergillus fumigatus, Aspergillus niger, Aspergillus ochraceus, Aspergillus versicolor, Penicillium funiculosum, Penicillium ochrochloron, Penicillium verrucosum var. cyclopium, and Trichoderma viride varied from 0.01 to 0.08 mg/mL, whereas the controls Bifonazole and ketoconazole ranged from 0.2 to 3.5 mg/ mL. The oleoresin obtained by supercritical carbon dioxide presented bacteriostatic, bactericidal, fungistatic, and fungicidal activities that were higher than the positive controls streptomycin, ampicillin, Bifonazole, and ketoconazole. The high antimicrobial activity was related to the high content of (E, E)-geranyllinalool that composes 21.0% of the oleoresin and a possible synergic action with fatty acid esters that made up 50.5% of the oleoresin. The oleoresin antimicrobial activity against common multiresistant bacteria in severe infectious processes as P. aeruginosa or against toxin-producing fungi such as P. ochrochloron or fungi that are difficult to control such as T. viride suggests the development of promising applications of this product in the food, farming, livestock, and pharmaceutical industry.Genetics and Molecular Research (2017), 16(2): gmr1602954

  • 4-Thiazolidinone derivatives as potent antimicrobial agents: microwave-assisted synthesis, biological evaluation and docking studies
    2015
    Co-Authors: Pitta Eleni, Geronikaki Athina, Soković Marina, Glamočlija Jasmina, Tsolaki Evangelia, Petrović Jovana, Crespan Emmanuele, Maga Giovanni, Bhunia, Shome S., Saxena, Anil K.
    Abstract:

    As a part of our ongoing research in the development of new antimicrobials, herein, we report the synthesis of ten compounds which combine three bioactive moieties: thiazole, adamantane and 4-thiazolidinone. Evaluation of their antibacterial activity revealed that the newly synthesized compounds exhibited remarkable growth inhibition of a wide spectrum of Gram-positive bacteria, Gram-negative bacteria and fungi. The majority of the compounds displayed greater antibacterial activity than the reference drugs (ampicillin and streptomycin), while the antifungal activity was significantly higher than that of the reference drugs Bifonazole and ketoconazole. Additionally, the title compounds were screened for HIV-1 reverse transcriptase inhibitory activity, showing no significant activity. Moreover, docking studies were performed in order to explore possible binding modes at the MurB protein of S. aureus

  • Bioactivity of the extracts and compounds of Ruscus aculeatus L. and Ruscus hypoglossum L.
    'Elsevier BV', 2013
    Co-Authors: Hadžifejzović Nihad, Soković Marina, Glamočlija Jasmina, Kukić-marković Jelena, Petrović Silvana, Stojković Dejan, Nahrstedt Adolf
    Abstract:

    Here we report on the antimicrobial and antioxidant activity of several extracts of Ruscus aculeatus L. and Ruscus hypoglossum L., as well as of some compounds previously isolated from them (p-coumaric and caffeic acids, rutin). For antimicrobial activity, the microdilution technique was used against eight bacterial and five fungal strains, along with standard drugs (streptomycin, ampicillin, Bifonazole, ketoconazole). Investigated extracts and isolated compounds showed antibacterial and especially antifungal activity. In some cases this activity was better than standard drugs (streptomycin, ampicillin, Bifonazole, ketoconazole) and was more evident for some of isolated compounds. Antioxidant activity was investigated by DPPH and 2-deoxyribose assays, along with spectrophotometrical determination of total phenolics content (TPC) using Folin-Ciocalteu reagent. In DPPH test obtained SC50 values were in a range of 173-541 mu g/mL, while inhibition of 2-deoxyribose for some extracts reached up to maximal 50%. Results of antioxidant assays were strongly correlated with total phenolics content. The most active was the EtOAc fraction of the MeOH extract of R. aculeatus herb regarding all investigated parameters

Glamočlija Jasmina - One of the best experts on this subject based on the ideXlab platform.

  • Antimicrobial activity and chemical composition of Brunfelsia uniflora flower oleoresin extracted by supercritical carbon dioxide
    'Genetics and Molecular Research', 2017
    Co-Authors: Thiesen L. C. T., Soković Marina, Tešević Vele, Glamočlija Jasmina, Sugauara E. Y. Y., Goncalves J. E., Gazim Z. C., Linde G. A., Colauto N. B.
    Abstract:

    Brunfelsia genus is traditionally utilized in popular medicine due to its antibacterial and antifungal properties to name but a few. However, studies on the antimicrobial activity of Brunfelsia uniflora flower oleoresin have not been found yet. This study aimed to evaluate the chemical composition and antimicrobial activity of B. uniflora flower oleoresin obtained by supercritical carbon dioxide. Oleoresin from the plant dried flowers was obtained by carbon dioxide, and the chemical composition was analyzed by gas chromatographic-mass spectrometry. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and minimum fungicidal concentration (MFC) of this oleoresin for seven bacteria and eight fungi were determined using 96-well microtiter plates. The oleoresin MBC for Bacillus cereus, Enterobacter cloacae, Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella enterica, and Staphylococcus aureus ranged from 0.01 to 0.08 mg/mL, whereas the controls streptomycin and ampicillin varied from 0.1 and 0.5 mg/mL. The oleoresin MFC for Aspergillus fumigatus, Aspergillus niger, Aspergillus ochraceus, Aspergillus versicolor, Penicillium funiculosum, Penicillium ochrochloron, Penicillium verrucosum var. cyclopium, and Trichoderma viride varied from 0.01 to 0.08 mg/mL, whereas the controls Bifonazole and ketoconazole ranged from 0.2 to 3.5 mg/mL. The oleoresin obtained by supercritical carbon dioxide presented bacteriostatic, bactericidal, fungistatic, and fungicidal activities that were higher than the positive controls streptomycin, ampicillin, Bifonazole, and ketoconazole. The high antimicrobial activity was related to the high content of (E, E)-geranyllinalool that composes 21.0% of the oleoresin and a possible synergic action with fatty acid esters that made up 50.5% of the oleoresin. The oleoresin antimicrobial activity against common multiresistant bacteria in severe infectious processes as P. aeruginosa or against toxin-producing fungi such as P. ochrochloron or fungi that are difficult to control such as T. viride suggests the development of promising applications of this product in the food, farming, livestock, and pharmaceutical industry

  • Antimicrobial activity and chemical composition of Brunfelsia uniflora flower oleoresin extracted by supercritical carbon dioxide
    2017
    Co-Authors: Thiesen, Letícia De Cassia Tavares, Soković Marina, Tešević Vele, Glamočlija Jasmina, Linde, Giani Andrea, Sugauara, Elaine Yae Yamashita, Gonçalves, José Eduardo, Gazim, Zilda Cristiani, Colauto Nelson Barros
    Abstract:

    Brunfelsia genus is traditionally utilized in popular medicine due to its antibacterial and antifungal properties to name but a few. However, studies on the antimicrobial activity of Brunfelsia uniflora flower oleoresin have not been found yet. This study aimed to evaluate the chemical composition and antimicrobial activity of B. uniflora flower oleoresin obtained by supercritical carbon dioxide. Oleoresin from the plant dried flowers was obtained by carbon dioxide, and the chemical composition was analyzed by gas chromatographic-mass spectrometry. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and minimum fungicidal concentration (MFC) of this oleoresin for seven bacteria and eight fungi were determined using 96-well microtiter plates. The oleoresin MBC for Bacillus cereus, Enterobacter cloacae, Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella enterica, and Staphylococcus aureus ranged from 0.01 to 0.08 mg/mL, whereas the controls streptomycin and ampicillin varied from 0.1 and 0.5 mg/ mL. The oleoresin MFC for Aspergillus fumigatus, Aspergillus niger, Aspergillus ochraceus, Aspergillus versicolor, Penicillium funiculosum, Penicillium ochrochloron, Penicillium verrucosum var. cyclopium, and Trichoderma viride varied from 0.01 to 0.08 mg/mL, whereas the controls Bifonazole and ketoconazole ranged from 0.2 to 3.5 mg/ mL. The oleoresin obtained by supercritical carbon dioxide presented bacteriostatic, bactericidal, fungistatic, and fungicidal activities that were higher than the positive controls streptomycin, ampicillin, Bifonazole, and ketoconazole. The high antimicrobial activity was related to the high content of (E, E)-geranyllinalool that composes 21.0% of the oleoresin and a possible synergic action with fatty acid esters that made up 50.5% of the oleoresin. The oleoresin antimicrobial activity against common multiresistant bacteria in severe infectious processes as P. aeruginosa or against toxin-producing fungi such as P. ochrochloron or fungi that are difficult to control such as T. viride suggests the development of promising applications of this product in the food, farming, livestock, and pharmaceutical industry.Genetics and Molecular Research (2017), 16(2): gmr1602954

  • 4-Thiazolidinone derivatives as potent antimicrobial agents: microwave-assisted synthesis, biological evaluation and docking studies
    2015
    Co-Authors: Pitta Eleni, Geronikaki Athina, Soković Marina, Glamočlija Jasmina, Tsolaki Evangelia, Petrović Jovana, Crespan Emmanuele, Maga Giovanni, Bhunia, Shome S., Saxena, Anil K.
    Abstract:

    As a part of our ongoing research in the development of new antimicrobials, herein, we report the synthesis of ten compounds which combine three bioactive moieties: thiazole, adamantane and 4-thiazolidinone. Evaluation of their antibacterial activity revealed that the newly synthesized compounds exhibited remarkable growth inhibition of a wide spectrum of Gram-positive bacteria, Gram-negative bacteria and fungi. The majority of the compounds displayed greater antibacterial activity than the reference drugs (ampicillin and streptomycin), while the antifungal activity was significantly higher than that of the reference drugs Bifonazole and ketoconazole. Additionally, the title compounds were screened for HIV-1 reverse transcriptase inhibitory activity, showing no significant activity. Moreover, docking studies were performed in order to explore possible binding modes at the MurB protein of S. aureus

  • Bioactivity of the extracts and compounds of Ruscus aculeatus L. and Ruscus hypoglossum L.
    'Elsevier BV', 2013
    Co-Authors: Hadžifejzović Nihad, Soković Marina, Glamočlija Jasmina, Kukić-marković Jelena, Petrović Silvana, Stojković Dejan, Nahrstedt Adolf
    Abstract:

    Here we report on the antimicrobial and antioxidant activity of several extracts of Ruscus aculeatus L. and Ruscus hypoglossum L., as well as of some compounds previously isolated from them (p-coumaric and caffeic acids, rutin). For antimicrobial activity, the microdilution technique was used against eight bacterial and five fungal strains, along with standard drugs (streptomycin, ampicillin, Bifonazole, ketoconazole). Investigated extracts and isolated compounds showed antibacterial and especially antifungal activity. In some cases this activity was better than standard drugs (streptomycin, ampicillin, Bifonazole, ketoconazole) and was more evident for some of isolated compounds. Antioxidant activity was investigated by DPPH and 2-deoxyribose assays, along with spectrophotometrical determination of total phenolics content (TPC) using Folin-Ciocalteu reagent. In DPPH test obtained SC50 values were in a range of 173-541 mu g/mL, while inhibition of 2-deoxyribose for some extracts reached up to maximal 50%. Results of antioxidant assays were strongly correlated with total phenolics content. The most active was the EtOAc fraction of the MeOH extract of R. aculeatus herb regarding all investigated parameters

  • Bioactivity of the extracts and compounds of Ruscus aculeatus L. and Ruscus hypoglossum L.
    2013
    Co-Authors: Hadžifejzović Nihad, Soković Marina, Glamočlija Jasmina, Stojković Dejan, Kukić-marković, Jelena M, Petrović, Silvana D, Nahrstedt Adolf
    Abstract:

    Here we report on the antimicrobial and antioxidant activity of several extracts of Ruscus aculeatus L. and Ruscus hypoglossum L., as well as of some compounds previously isolated from them (p-coumaric and caffeic acids, rutin). For antimicrobial activity, the microdilution technique was used against eight bacterial and five fungal strains, along with standard drugs (streptomycin, ampicillin, Bifonazole, ketoconazole). Investigated extracts and isolated compounds showed antibacterial and especially antifungal activity. In some cases this activity was better than standard drugs (streptomycin, ampicillin, Bifonazole, ketoconazole) and was more evident for some of isolated compounds. Antioxidant activity was investigated by DPPH and 2-deoxyribose assays, along with spectrophotometrical determination of total phenolics content (TPC) using Folin-Ciocalteu reagent. In DPPH test obtained SC50 values were in a range of 173-541 mu g/mL, while inhibition of 2-deoxyribose for some extracts reached up to maximal 50%. Results of antioxidant assays were strongly correlated with total phenolics content. The most active was the EtOAc fraction of the MeOH extract of R. aculeatus herb regarding all investigated parameters. (C) 2013 Elsevier B.V. All rights reserved.Ministry of Education and Science of Republic of Serbia [173021, 173032

Tešević Vele - One of the best experts on this subject based on the ideXlab platform.

  • Antimicrobial activity and chemical composition of Brunfelsia uniflora flower oleoresin extracted by supercritical carbon dioxide
    2017
    Co-Authors: Thiesen, Letícia De Cassia Tavares, Soković Marina, Tešević Vele, Glamočlija Jasmina, Linde, Giani Andrea, Sugauara, Elaine Yae Yamashita, Gonçalves, José Eduardo, Gazim, Zilda Cristiani, Colauto Nelson Barros
    Abstract:

    Brunfelsia genus is traditionally utilized in popular medicine due to its antibacterial and antifungal properties to name but a few. However, studies on the antimicrobial activity of Brunfelsia uniflora flower oleoresin have not been found yet. This study aimed to evaluate the chemical composition and antimicrobial activity of B. uniflora flower oleoresin obtained by supercritical carbon dioxide. Oleoresin from the plant dried flowers was obtained by carbon dioxide, and the chemical composition was analyzed by gas chromatographic-mass spectrometry. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and minimum fungicidal concentration (MFC) of this oleoresin for seven bacteria and eight fungi were determined using 96-well microtiter plates. The oleoresin MBC for Bacillus cereus, Enterobacter cloacae, Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella enterica, and Staphylococcus aureus ranged from 0.01 to 0.08 mg/mL, whereas the controls streptomycin and ampicillin varied from 0.1 and 0.5 mg/ mL. The oleoresin MFC for Aspergillus fumigatus, Aspergillus niger, Aspergillus ochraceus, Aspergillus versicolor, Penicillium funiculosum, Penicillium ochrochloron, Penicillium verrucosum var. cyclopium, and Trichoderma viride varied from 0.01 to 0.08 mg/mL, whereas the controls Bifonazole and ketoconazole ranged from 0.2 to 3.5 mg/ mL. The oleoresin obtained by supercritical carbon dioxide presented bacteriostatic, bactericidal, fungistatic, and fungicidal activities that were higher than the positive controls streptomycin, ampicillin, Bifonazole, and ketoconazole. The high antimicrobial activity was related to the high content of (E, E)-geranyllinalool that composes 21.0% of the oleoresin and a possible synergic action with fatty acid esters that made up 50.5% of the oleoresin. The oleoresin antimicrobial activity against common multiresistant bacteria in severe infectious processes as P. aeruginosa or against toxin-producing fungi such as P. ochrochloron or fungi that are difficult to control such as T. viride suggests the development of promising applications of this product in the food, farming, livestock, and pharmaceutical industry.Genetics and Molecular Research (2017), 16(2): gmr1602954

  • Antimicrobial activity and chemical composition of Brunfelsia uniflora flower oleoresin extracted by supercritical carbon dioxide
    'Genetics and Molecular Research', 2017
    Co-Authors: Thiesen L. C. T., Soković Marina, Tešević Vele, Glamočlija Jasmina, Sugauara E. Y. Y., Goncalves J. E., Gazim Z. C., Linde G. A., Colauto N. B.
    Abstract:

    Brunfelsia genus is traditionally utilized in popular medicine due to its antibacterial and antifungal properties to name but a few. However, studies on the antimicrobial activity of Brunfelsia uniflora flower oleoresin have not been found yet. This study aimed to evaluate the chemical composition and antimicrobial activity of B. uniflora flower oleoresin obtained by supercritical carbon dioxide. Oleoresin from the plant dried flowers was obtained by carbon dioxide, and the chemical composition was analyzed by gas chromatographic-mass spectrometry. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and minimum fungicidal concentration (MFC) of this oleoresin for seven bacteria and eight fungi were determined using 96-well microtiter plates. The oleoresin MBC for Bacillus cereus, Enterobacter cloacae, Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella enterica, and Staphylococcus aureus ranged from 0.01 to 0.08 mg/mL, whereas the controls streptomycin and ampicillin varied from 0.1 and 0.5 mg/mL. The oleoresin MFC for Aspergillus fumigatus, Aspergillus niger, Aspergillus ochraceus, Aspergillus versicolor, Penicillium funiculosum, Penicillium ochrochloron, Penicillium verrucosum var. cyclopium, and Trichoderma viride varied from 0.01 to 0.08 mg/mL, whereas the controls Bifonazole and ketoconazole ranged from 0.2 to 3.5 mg/mL. The oleoresin obtained by supercritical carbon dioxide presented bacteriostatic, bactericidal, fungistatic, and fungicidal activities that were higher than the positive controls streptomycin, ampicillin, Bifonazole, and ketoconazole. The high antimicrobial activity was related to the high content of (E, E)-geranyllinalool that composes 21.0% of the oleoresin and a possible synergic action with fatty acid esters that made up 50.5% of the oleoresin. The oleoresin antimicrobial activity against common multiresistant bacteria in severe infectious processes as P. aeruginosa or against toxin-producing fungi such as P. ochrochloron or fungi that are difficult to control such as T. viride suggests the development of promising applications of this product in the food, farming, livestock, and pharmaceutical industry

  • Chemical Characterization of Lippia Alba Essential Oil: An Alternative To Control Green Molds
    2011
    Co-Authors: Glamočlija Jasmina, Soković Marina, Tešević Vele, Linde, Giani Andrea, Colauto Nelson Barros
    Abstract:

    The essential oil of Lippia alba is reported as an antifungal against human pathogenic microorganisms but few articles report its use as an alternative to synthetic fungicides on green mould control. The objective of this study was to determine chemical characteristics of L. alba essential oil and its antifungal activity against green molds as an alternative to synthetic fungicides. Essential oil was extracted by Clevenger hydrodistillation, characterized by GC-MS analysis, and the structure of the main compounds confirmed by H-1 and C-13-NMR spectroscopy. Microdilution assays evaluated the essential oil minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC). Commercial fungicides Ketoconazole and Bifonazole were used as control. Essential oil yield is of 0.15% and the major components are neral (33.32%) and geranial (50.94%). The L. alba essential oil has MIC of 0.300-1.250 mg/mL and MFC of 0.600-1.250 mg/mL. Ketoconazole and Bifonazole show MIC ranging from 0.025-0.500 to 0.100-0.200 mg/mL, and MFC ranging from 0.250-0.100 to 0.200-0.250 mg/mL, respectively. L. alba essential oil is classified as citral type and the results indicate that it is a potential alternative to synthetic fungicides.Ministry of Science and Technological Development of the Republic of Serbia [173032, 172053]; Paranaense Universit

  • Antifungalna i antioksidativna aktivnost etarskog ulja Mentha longifolia (L.) Huds. (Lamiaceae)
    2010
    Co-Authors: Džamić, Ana M., Soković, Marina D., Ristić, Mihailo S., Novaković, Miroslav M., Grujić-jovanović Slavica, Tešević Vele, Marin, Petar D.
    Abstract:

    The present study describes the antifungal and antioxidant activity of Mentha longifolia (L.) Hudson essential oil. This plant is native to Europe, Central Asia and Australia. It is used as carminative, stomachic and stimulant and also in aromatherapy. The essential oil profile was determined by GC and GC-MS. The main compounds in the oil were trans-dihydrocarvone (23.64%), piperitone (17.33%) and cis-dihydrocarvone (15.68%). Minimum inhibitory concentrations (MIC) and minimum fungicidal concentrations (MFC) were recorded using the microdilution method. Commercial antimicotic bifonazol was used as a control. The concentration of 10 μl/ml showed fungicidal activity against Aspergillus and Fusarium species, Penicillium funiculosum and Trichoderma viride. Concentration of 5 μl/ml was efficient against Trichophyton menthagrophytes and yeast Candida albicans. The most sensitive micromycetes were Cladosporium fulvum, C. cladosporium cladosporioides and Penicillium ochrochloron where concentration of 2.5 μl/ml was lethal. The antioxidant activity of essential oil was evaluated by means of the 2,2-diphenyl-1-picrylhydrazil (DPPH) radical scavenging method. The essential oil of M. longifolia was able to reduce DPPH radicals into the DPPH-H form, and this activity was dose-dependent. The oil exhibited significant potential for antioxidant activity, and reduced DPPH to 50% (IC50=0,659 ml/ml of solution). .U radu je ispitivano potencijalno delovanje etarskog ulja Mentha longifolia (L.) Huds. kao antifungalnog i antioksidativnog agensa. Vrsta M. longifolia je samonikla u Evropi, Centralnoj Aziji i Australiji. Koristi se kao karminativ, stomahik i stimulant u aromaterapiji. Kompozicija etarskog ulja je određivana korišćenjem gasne hromatografi je (GH) i gasne hromatografije sa masenom spektroskopijom (GH-MS). Dominantne komponente u ispitivanom etarskom ulju su: trans-dihidrokarvon (23.64%), piperiton (17.33%) i cis-dihidrokarvon (15.68%). Antifungalna aktivnost je ispitivana metodom mikrodilucije i određivane su minimalne inhibitorne i fungicidne koncentracije ulja i komercijalnog fungicida bifonazola. Etarsko ulje je pokazalo fungicidno dejstvo pri koncentraciji 10 μl/ml za gljive iz rodova Aspergillus i Fusarium kao i za vrste Penicillium funiculosum i Trichoderma viride. Najosetljivije su mikromicete Cladosporium fulvum, C. cladosporioides i Penicillium ochrochloron, za koje je letalna koncentracija 2.5 μl/ml. Antioksidativna aktivnost je rađena korišćenjem DPPH kao hvatača slobodnih radikala. Etarsko ulje M. longifolia je pokazalo sposobnost redukcije DPPH radikala u DPPH-H formu. Antifungalna aktivnost je predstavljena redukcijom DPPH na 50% (IC50=0,659 ml/ml rastvora)

  • Antifungal and antioxidant activity of Mentha longifolia (L.) Hudson (Lamiaceae) essential oil
    2010
    Co-Authors: Džamić, Ana M., Soković Marina, Ristić, Mihailo S., Tešević Vele, Novaković Miroslav, Grujić-jovanović, Slavica M., Marin, Petar D.
    Abstract:

    The present study describes the antifungal and antioxidant activity of Mentha longifolia (L.) Hudson essential oil. This plant is native to Europe, Central Asia and Australia. It is used as carminative, stomachic and stimulant and also in aromatherapy. The essential oil profile was determined by GC and GC-MS. The main compounds in the oil were trans-dihydrocarvone (23.64%), piperitone (17.33%) and cis-dihydrocarvone (15.68%). Minimum inhibitory concentrations (MIC) and minimum fungicidal concentrations (MFC) were recorded using the microdilution method. Commercial antimicotic bifonazol was used as a control. The concentration of 10 μl/ml showed fungicidal activity against Aspergillus and Fusarium species, Penicillium funiculosum and Trichoderma viride. Concentration of 5 μl/ml was efficient against Trichophyton menthagrophytes and yeast Candida albicans. The most sensitive micromycetes were Cladosporium fulvum, C. cladosporium cladosporioides and Penicillium ochrochloron where concentration of 2.5 μl/ml was lethal. The antioxidant activity of essential oil was evaluated by means of the 2,2-diphenyl-1-picrylhydrazil (DPPH) radical scavenging method. The essential oil of M. longifolia was able to reduce DPPH radicals into the DPPH-H form, and this activity was dose-dependent. The oil exhibited significant potential for antioxidant activity, and reduced DPPH to 50% (IC50=0,659 ml/ml of solution). .U radu je ispitivano potencijalno delovanje etarskog ulja Mentha longifolia (L.) Huds. kao antifungalnog i antioksidativnog agensa. Vrsta M. longifolia je samonikla u Evropi, Centralnoj Aziji i Australiji. Koristi se kao karminativ, stomahik i stimulant u aromaterapiji. Kompozicija etarskog ulja je određivana korišćenjem gasne hromatografi je (GH) i gasne hromatografije sa masenom spektroskopijom (GH-MS). Dominantne komponente u ispitivanom etarskom ulju su: trans-dihidrokarvon (23.64%), piperiton (17.33%) i cis-dihidrokarvon (15.68%). Antifungalna aktivnost je ispitivana metodom mikrodilucije i određivane su minimalne inhibitorne i fungicidne koncentracije ulja i komercijalnog fungicida bifonazola. Etarsko ulje je pokazalo fungicidno dejstvo pri koncentraciji 10 μl/ml za gljive iz rodova Aspergillus i Fusarium kao i za vrste Penicillium funiculosum i Trichoderma viride. Najosetljivije su mikromicete Cladosporium fulvum, C. cladosporioides i Penicillium ochrochloron, za koje je letalna koncentracija 2.5 μl/ml. Antioksidativna aktivnost je rađena korišćenjem DPPH kao hvatača slobodnih radikala. Etarsko ulje M. longifolia je pokazalo sposobnost redukcije DPPH radikala u DPPH-H formu. Antifungalna aktivnost je predstavljena redukcijom DPPH na 50% (IC50=0,659 ml/ml rastvora).nul

Shome S Bhunia - One of the best experts on this subject based on the ideXlab platform.

  • 4 thiazolidinone derivatives as potent antimicrobial agents microwave assisted synthesis biological evaluation and docking studies
    MedChemComm, 2015
    Co-Authors: Eleni Pitta, Athina Geronikaki, Marina Sokovic, Evangelia Tsolaki, Jovana Petrovic, Jasmina Glamoclija, Emmanuele Crespan, Giovanni Maga, Shome S Bhunia
    Abstract:

    As a part of our ongoing research in the development of new antimicrobials, herein, we report the synthesis of ten compounds which combine three bioactive moieties: thiazole, adamantane and 4-thiazolidinone. Evaluation of their antibacterial activity revealed that the newly synthesized compounds exhibited remarkable growth inhibition of a wide spectrum of Gram-positive bacteria, Gram-negative bacteria and fungi. The majority of the compounds displayed greater antibacterial activity than the reference drugs (ampicillin and streptomycin), while the antifungal activity was significantly higher than that of the reference drugs Bifonazole and ketoconazole. Additionally, the title compounds were screened for HIV-1 reverse transcriptase inhibitory activity, showing no significant activity. Moreover, docking studies were performed in order to explore possible binding modes at the MurB protein of S. aureus.

Marina Sokovic - One of the best experts on this subject based on the ideXlab platform.

  • 4 thiazolidinone derivatives as potent antimicrobial agents microwave assisted synthesis biological evaluation and docking studies
    MedChemComm, 2015
    Co-Authors: Eleni Pitta, Athina Geronikaki, Marina Sokovic, Evangelia Tsolaki, Jovana Petrovic, Jasmina Glamoclija, Emmanuele Crespan, Giovanni Maga, Shome S Bhunia
    Abstract:

    As a part of our ongoing research in the development of new antimicrobials, herein, we report the synthesis of ten compounds which combine three bioactive moieties: thiazole, adamantane and 4-thiazolidinone. Evaluation of their antibacterial activity revealed that the newly synthesized compounds exhibited remarkable growth inhibition of a wide spectrum of Gram-positive bacteria, Gram-negative bacteria and fungi. The majority of the compounds displayed greater antibacterial activity than the reference drugs (ampicillin and streptomycin), while the antifungal activity was significantly higher than that of the reference drugs Bifonazole and ketoconazole. Additionally, the title compounds were screened for HIV-1 reverse transcriptase inhibitory activity, showing no significant activity. Moreover, docking studies were performed in order to explore possible binding modes at the MurB protein of S. aureus.

  • sulfonamide 1 2 4 thiadiazole derivatives as antifungal and antibacterial agents synthesis biological evaluation lipophilicity and conformational studies
    Chemical & Pharmaceutical Bulletin, 2010
    Co-Authors: Charalabos Camoutsis, Athina Geronikaki, Ana Ciric, Marina Sokovic, Panagiotis Zoumpoulakis, Maria Zervou
    Abstract:

    A series of thirteen new thiadiazole compounds were synthesized and evaluated for in vitro antifungal and antibacterial activity. All compound tested showed significant antifungal activity against all the micromycetes, compared to the commercial fungicide Bifonazole. Differences in their activity depend on the substitution of different reactive groups. More specifically, best antifungal activity was shown for the synthetic analogue with methylpiperazine reactive group. Furthermore, it is apparent that different compounds reacted on different ways against bacteria. An effort was made to correlate the above mentioned differences in activity with lipophilicity studies. Furthermore, NMR and molecular modelling were used to obtain the main conformational features of a potent analogue, for future in silico studies.

  • antibacterial and antifungal activity of the liverwort ptilidium pulcherrimum methanol extract
    Archives of Biological Sciences, 2010
    Co-Authors: Milan Veljic, Ana Ciric, Marina Sokovic, Pedja Janackovic, Petar D Marin
    Abstract:

    The antimicrobial activity of the methanol extract from the liverwort, Ptilidium pulcherrimum was evaluated against five bacterial and six fungal species. In vitro antibacterial activity was assessed by disc diffusion and micro- dilution methods. The extract showed a stronger effect against tested Gram (+) than Gram (-) bacteria. The antifungal activity of the methanol extract was tested using a microdilution method. The methanol extract showed strong antifungal activity. The best antifungal activity was achieved against Trichoderma viride compared to the synthetic fungicide bifonazol.

  • sulfonamide 1 2 4 triazole derivatives as antifungal and antibacterial agents synthesis biological evaluation lipophilicity and conformational studies
    Bioorganic & Medicinal Chemistry, 2008
    Co-Authors: Iraj Rahavi Ezabadi, Charalabos Camoutsis, Athina Geronikaki, Marina Sokovic, Panagiotis Zoumpoulakis, Jasmina Glamocilija, Ana Ciric
    Abstract:

    A series of thirteen new thiadiazole compounds were synthesized and evaluated for in vitro antifungal and antibacterial activity. All compound tested showed significant antifungal activity against all the micromycetes, compared to the commercial fungicide Bifonazole. Differences in their activity depend on the substitution of different reactive groups. More specifically, best antifungal activity was shown for the synthetic analogue with methylpiperazine reactive group. Furthermore, it is apparent that different compounds reacted on different ways against bacteria. An effort was made to correlate the above mentioned differences in activity with lipophilicity studies. Furthermore, NMR and molecular modelling were used to obtain the main conformational features of a potent analogue, for future in silico studies.

  • Chemical composition, antibacterial and antifungal activity of the essential oils of Cotinus coggygria from Serbia
    Serbian Chemical Society, 2007
    Co-Authors: Miroslav Novakovic, Marina Sokovic, Vele Tesevic, Pedja Janackovic, Ivan Vuckovic, Anka Filipovic, Slobodan Milosavljevic
    Abstract:

    Essential oils from leaves with young branches of Cotinus coggygria Scop. from two localities in Serbia (Deliblatska pescara and Zemun), obtained by hydrodistillation, were analysed by GC–MS. Thirty-one component were identified from both oils and among them monoterpenic hydrocarbons were the dominant class (87.4 and 93.1 %). The dominant constituent in both essential oils was limonene (47.0 and 39.2 %). Both oils were also tested for antibacterial and antifungal activities. In comparison to streptomycin, both oils showed slightly higher activity (against most Gram-positive bacteria) in the disc diffusion method and slightly lower activity when the microdilution method was employed. They also exhibited antifungal potential higher than that of the commercial fungicide Bifonazole