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Jiang Chang - One of the best experts on this subject based on the ideXlab platform.

  • The stimulation of osteogenic differentiation of embryoid bodies from human induced pluripotent stem cells by akermanite bioceramics
    Journal of Materials Chemistry B, 2016
    Co-Authors: Xixi Dong, Haiyan Li, Yanling Zhou, Long Ou, Jiang Chang
    Abstract:

    Induced pluripotent stem cells (iPSCs) have great potential as seed cells for tissue engineering applications. Previous studies have shown that iPSCs could be induced to differentiate into bone forming cells. However, in a tissue engineering approach, seeding cells in bioMaterials is required, and the effect of bioMaterials on cell growth and differentiation is critical for the success of the formation of engineered tissues. In this study, we investigated the effect of akermanite, a Bioactive ceramic, on the osteogenic differentiation of embryoid body (EB) cells derived from human iPSCs. The results showed that, in the presence of osteogenic factors (ascorbic acid, dexamethasone, and β-glycerophosphate), ionic extracts of akermanite enhanced the osteogenic differentiation of EB cells as compared with normal osteogenic medium. Alkaline phosphatase (ALP) activity and the expression of osteogenic marker genes such as osteocalcin (OCN), collagen (COL-1), RUNX2, and BMP2 are significantly increased by the stimulation of akermanite ceramic extracts at certain concentration ranges. More interesting is that the medium containing extracts of akermanite but without osteogenic factors also showed stimulatory effects on the osteogenic differentiation of EB cells as compared to normal growth medium without osteogenic factors, such as ascorbic acid, dexamethasone, and β-glycerophosphate, not at the early stage of culture, but only at the later stage of the culture period (21 days). These results suggest that akermanite as a Bioactive Material together with human iPSCs might be used for bone tissue engineering applications.

  • novel tricalcium silicate magnesium phosphate composite bone cement having high compressive strength in vitro bioactivity and cytocompatibility
    Acta Biomaterialia, 2015
    Co-Authors: Wenjuan Liu, Dong Zhai, Zhiguang Huan, Jiang Chang
    Abstract:

    Abstract Although inorganic bone cements such as calcium phosphate cements have been widely applied in orthopaedic and dental fields because of their self-setting ability, development of high-strength bone cement with bioactivity and biodegradability remains a major challenge. Therefore, the purpose of this study is to prepare a tricalcium silicate/magnesium phosphate (C3S/MPC) composite bone cement, which is intended to combine the excellent bioactivity of C3S with remarkable self-setting properties and mechanical strength of MPC. The self-setting and mechanical properties, in vitro induction of apatite formation and degradation behaviour, and cytocompatibility of the composite cements were investigated. Our results showed that the C3S/MPC composite cement with an optimal composition had compressive strength up to 87 MPa, which was significantly higher than C3S (25 MPa) and MPC (64 MPa). The setting time could be adjusted between 3 min and 29 min with the variation of compositions. The hydraulic reaction products of the C3S/MPC composite cement were composed of calcium silicate hydrate (CSH) derived from the hydration of C3S and gel-like amorphous substance. The C3S/MPC composite cements could induce apatite mineralization on its surface in SBF solution and degraded gradually in Tris–HCl solution. Besides, the composite cements showed good cytocompatibility and stimulatory effect on the proliferation of MC3T3-E1 osteoblast cells. Our results indicated that the C3S/MPC composite bone cement might be a new promising high-strength inorganic Bioactive Material which may hold the potential for bone repair in load-bearing site.

  • effect of tricalcium silicate ca3sio5 Bioactive Material on reducing enamel demineralization an in vitro ph cycling study
    Journal of Dentistry, 2012
    Co-Authors: Yueyue Wang, Jiang Chang, Yan Deng
    Abstract:

    Abstract Objectives The aim of this study was to investigate the effect of Ca 3 SiO 5 on reducing enamel demineralization under pH-cycling conditions. Methods Forty bovine enamel samples were treated under four conditions: group 1, double distilled water (negative control); group 2, 1000 ppm F (as NaF, positive control); group 3, Ca 3 SiO 5 slurry; and group 4, Ca 3 SiO 5 –F slurry (Ca 3 SiO 5 with 1000 ppm F aq.). All the specimens were treated with treatment Materials 4 times each day. Samples in groups 1 and 2 were soaked in test solutions for 2 min and samples in groups 3 and 4 were painted in treatment slurry for 2 min. At times between treatments, they were immersed in citric acid solution 3 times a day and 15 s each time. All the procedures were repeated for 7 days. Knoop microhardness, scanning electron microscopy (SEM), X-ray diffraction (XRD) and atom force microscopy (AFM) were used to examine samples. Results After treatment for 7 days, enamels in all the groups were significantly softened. The extents of microhardness reduction were 52.3%, 28.5%, 28.5% and 20.2% for groups 1, 2, 3 and 4, respectively. Samples in the negative control group showed a typical acid etched pattern while enamels in other groups were relatively compact. There was no significant difference between samples treated with Ca 3 SiO 5 and F. The combination of Ca 3 SiO 5 with F showed the best effect on reducing enamel demineralization. Conclusions Ca 3 SiO 5 is an effective Material against enamel demineralization alone but in combination with F a better anti-demineralization effect may be obtained.

  • strontium containing mesoporous Bioactive glass scaffolds with improved osteogenic cementogenic differentiation of periodontal ligament cells for periodontal tissue engineering
    Institute of Health and Biomedical Innovation; Science & Engineering Faculty, 2012
    Co-Authors: Yinghong Zhou, Jiang Chang, Chucheng Lin, Yin Xiao
    Abstract:

    To achieve the ultimate goal of periodontal tissue engineering, it is of great importance to develop Bioactive scaffolds which could stimulate the osteogenic/cementogenic differentiation of periodontal ligament cells (PDLCs) for the favorable regeneration of alveolar bone, root cementum, and periodontal ligament. Strontium (Sr) and Sr-containing bioMaterials have been found to induce osteoblast activity. However, there is no systematic report about the interaction between Sr or Sr-containing bioMaterials and PDLCs for periodontal tissue engineering. The aims of this study were to prepare Sr-containing mesoporous Bioactive glass (Sr-MBG) scaffolds and investigate whether the addition of Sr could stimulate the osteogenic/cementogenic differentiation of PDLCs in tissue engineering scaffold system. The composition, microstructure and mesopore properties (specific surface area, nano-pore volume and nano-pore distribution) of Sr-MBG scaffolds were characterized. The proliferation, alkaline phosphatase (ALP) activity and osteogenesis/cementogenesis-related gene expression (ALP, Runx2, Col I, OPN and CEMP1) of PDLCs on different kinds of Sr-MBG scaffolds were systematically investigated. The results show that Sr plays an important role in influencing the mesoporous structure of MBG scaffolds in which high contents of Sr decreased the well-ordered mesopores as well as their surface area/pore volume. Sr2+ ions could be released from Sr-MBG scaffolds in a controlled way. The incorporation of Sr into MBG scaffolds has significantly stimulated ALP activity and osteogenesis/cementogenesis-related gene expression of PDLCs. Furthermore, Sr-MBG scaffolds in simulated body fluids environment still maintained excellent apatite-mineralization ability. The study suggests that the incorporation of Sr into MBG scaffolds is a viable way to stimulate the biological response of PDLCs. Sr-MBG scaffolds are a promising Bioactive Material for periodontal tissue engineering application.

Marcelle Machluf - One of the best experts on this subject based on the ideXlab platform.

  • electrospun extracellular matrix paving the way to tailor made natural scaffolds for cardiac tissue regeneration
    Advanced Functional Materials, 2017
    Co-Authors: Beth Schoen, Lior Gepstein, Ron Avrahami, Limor Baruch, Yael Efraim, Idit Goldfracht, Ofek Elul, Tzila Davidov, Eyal Zussman, Marcelle Machluf
    Abstract:

    Biomimetic scaffolds generally aim at structurally and compositionally imitating native tissue, thus providing a supportive microenvironment to the transplanted or recruited cells in the tissue. Native decellularized porcine extracellular matrix (ECM) is becoming the ultimate Bioactive Material for the regeneration of different organs. Particularly for cardiac regeneration, ECM is studied as a patch and injectable scaffolds, which improve cardiac function, yet lack reproducibility and are difficult to control or fine-tune for the desired properties, like most natural Materials. Seeking to harness the natural advantages of ECM in a reproducible, scalable, and controllable scaffold, for the first time, a matrix that is produced from whole decellularized porcine cardiac ECM using electrospinning technology, is developed. This unique electrospun cardiac ECM mat preserves the composition of ECM, self-assembles into the same microstructure of cardiac ECM ,and ,above all, preserves key cardiac mechanical properties. It supports cell growth and function, and demonstrates biocompatibility in vitro and in vivo. Importantly, this work reveals the great potential of electrospun ECM-based platforms for a wide span of biomedical applications, thus offering the possibility to produce complex natural Materials as tailor-made, well-defined structures.

  • Electrospun Extracellular Matrix: Paving the Way to Tailor‐Made Natural Scaffolds for Cardiac Tissue Regeneration
    Advanced Functional Materials, 2017
    Co-Authors: Beth Schoen, Lior Gepstein, Ron Avrahami, Limor Baruch, Yael Efraim, Idit Goldfracht, Ofek Elul, Tzila Davidov, Eyal Zussman, Marcelle Machluf
    Abstract:

    Biomimetic scaffolds generally aim at structurally and compositionally imitating native tissue, thus providing a supportive microenvironment to the transplanted or recruited cells in the tissue. Native decellularized porcine extracellular matrix (ECM) is becoming the ultimate Bioactive Material for the regeneration of different organs. Particularly for cardiac regeneration, ECM is studied as a patch and injectable scaffolds, which improve cardiac function, yet lack reproducibility and are difficult to control or fine-tune for the desired properties, like most natural Materials. Seeking to harness the natural advantages of ECM in a reproducible, scalable, and controllable scaffold, for the first time, a matrix that is produced from whole decellularized porcine cardiac ECM using electrospinning technology, is developed. This unique electrospun cardiac ECM mat preserves the composition of ECM, self-assembles into the same microstructure of cardiac ECM ,and ,above all, preserves key cardiac mechanical properties. It supports cell growth and function, and demonstrates biocompatibility in vitro and in vivo. Importantly, this work reveals the great potential of electrospun ECM-based platforms for a wide span of biomedical applications, thus offering the possibility to produce complex natural Materials as tailor-made, well-defined structures.

Beth Schoen - One of the best experts on this subject based on the ideXlab platform.

  • electrospun extracellular matrix paving the way to tailor made natural scaffolds for cardiac tissue regeneration
    Advanced Functional Materials, 2017
    Co-Authors: Beth Schoen, Lior Gepstein, Ron Avrahami, Limor Baruch, Yael Efraim, Idit Goldfracht, Ofek Elul, Tzila Davidov, Eyal Zussman, Marcelle Machluf
    Abstract:

    Biomimetic scaffolds generally aim at structurally and compositionally imitating native tissue, thus providing a supportive microenvironment to the transplanted or recruited cells in the tissue. Native decellularized porcine extracellular matrix (ECM) is becoming the ultimate Bioactive Material for the regeneration of different organs. Particularly for cardiac regeneration, ECM is studied as a patch and injectable scaffolds, which improve cardiac function, yet lack reproducibility and are difficult to control or fine-tune for the desired properties, like most natural Materials. Seeking to harness the natural advantages of ECM in a reproducible, scalable, and controllable scaffold, for the first time, a matrix that is produced from whole decellularized porcine cardiac ECM using electrospinning technology, is developed. This unique electrospun cardiac ECM mat preserves the composition of ECM, self-assembles into the same microstructure of cardiac ECM ,and ,above all, preserves key cardiac mechanical properties. It supports cell growth and function, and demonstrates biocompatibility in vitro and in vivo. Importantly, this work reveals the great potential of electrospun ECM-based platforms for a wide span of biomedical applications, thus offering the possibility to produce complex natural Materials as tailor-made, well-defined structures.

  • Electrospun Extracellular Matrix: Paving the Way to Tailor‐Made Natural Scaffolds for Cardiac Tissue Regeneration
    Advanced Functional Materials, 2017
    Co-Authors: Beth Schoen, Lior Gepstein, Ron Avrahami, Limor Baruch, Yael Efraim, Idit Goldfracht, Ofek Elul, Tzila Davidov, Eyal Zussman, Marcelle Machluf
    Abstract:

    Biomimetic scaffolds generally aim at structurally and compositionally imitating native tissue, thus providing a supportive microenvironment to the transplanted or recruited cells in the tissue. Native decellularized porcine extracellular matrix (ECM) is becoming the ultimate Bioactive Material for the regeneration of different organs. Particularly for cardiac regeneration, ECM is studied as a patch and injectable scaffolds, which improve cardiac function, yet lack reproducibility and are difficult to control or fine-tune for the desired properties, like most natural Materials. Seeking to harness the natural advantages of ECM in a reproducible, scalable, and controllable scaffold, for the first time, a matrix that is produced from whole decellularized porcine cardiac ECM using electrospinning technology, is developed. This unique electrospun cardiac ECM mat preserves the composition of ECM, self-assembles into the same microstructure of cardiac ECM ,and ,above all, preserves key cardiac mechanical properties. It supports cell growth and function, and demonstrates biocompatibility in vitro and in vivo. Importantly, this work reveals the great potential of electrospun ECM-based platforms for a wide span of biomedical applications, thus offering the possibility to produce complex natural Materials as tailor-made, well-defined structures.

Shinnjyh Ding - One of the best experts on this subject based on the ideXlab platform.

  • stem cell differentiation induced calcium silicate cement with bacteriostatic activity
    Journal of Materials Chemistry B, 2015
    Co-Authors: Shu-ching Huang, Shinnjyh Ding
    Abstract:

    Calcium-based bone cements are widely used in dental and orthopaedic surgery. Those based on calcium phosphate (CPCs) or calcium silicate (CSCs) have a number of favourable properties that encourage their clinical use in bone defect repair. The purpose of the present study was to compare the in vitro osteogenesis and bacteriostatic activity of BoneSource CPCs with home-made CSCs, particularly in regard to their facility for cell differentiation. Cement in vitro osteogenic activity was evaluated by incubating the cement specimens with human mesenchymal stem cells (hMSCs). The bacteriostatic activity of the two cements against Gram-positive (S. aureus) and Gram-negative (P. aeruginosa) bacterial strains was assessed using a bacteriostasis ratio assay and by inhibition zone examination. Compared with CPC, CSC was shown to promote greater proliferation and osteogenic differentiation (alkaline phosphatase and osteocalcin), and the formation of mineralization nodules of hMSCs. It is worth noting that CSC could effectively induce hMSC differentiation, even when the culture medium did not contain osteogenetic differentiation agents. Compared with CPC, CSC also showed significantly greater bacteriostatic activity, as revealed by inhibition zones and the bacteriostasis ratio. Our findings suggest that CSC is a useful Bioactive Material for bone repair in terms of inducing cell differentiation, and may be considered an alternative to CPCs.

Ahmed Salama - One of the best experts on this subject based on the ideXlab platform.

  • Soy protein hydrolysate grafted cellulose nanofibrils with Bioactive signals for bone repair and regeneration.
    Carbohydrate polymers, 2019
    Co-Authors: Ahmed Salama, Ragab E. Abou-zeid, Iriczalli Cruz-maya, Vincenzo Guarino
    Abstract:

    TEMPO oxidized cellulose nanofibers (T-CNF) were prepared from cellulose pulp which is extracted from bagasse. Soy protein hydrolysate (SPH) was grafted on T-CNF via amidation of carboxylic groups. Biomineralization was, then, assessed via calcium phosphates (CaP) precipitation in twice-simulated body fluid until formation of a new Bioactive Material. Protein was efficiently grafted without alteration of morphology and nanofibrils packing as reported by Fourier Transform infrared analysis /X Ray Diffraction /Scanning and Transmission Electron Microscopy / Atomic Force Microscopy. Highly crystalline calcium phosphate deposits - ca. 22.1% - were detected, with a Ca/P ratio equal to 1.63, in agreement with native bone apatite composition. In vitro response of human Mesenchymal Stem Cells confirmed the biocompatibility. No significant differences in terms of cell adhesion were recognized while a significant increase in cell proliferation was detected until 7 days. The presence of calcium phosphates tends to cover the nanofibrillar pattern, inducing the inhibition of cell proliferation and promoting the ex-novo precipitation of mineral phases. All the results suggest a promising use of these bioMaterials in the repair and/or the regeneration of hard tissues such as bone.

  • Bioactive cellulose grafted soy protein isolate towards biomimetic calcium phosphate mineralization
    Industrial Crops and Products, 2017
    Co-Authors: Ahmed Salama, Nadia Shukry, Ahmed S Elgendy, Mohamed Elsakhawy
    Abstract:

    Abstract The current study is a new approach to prepare an environmentally friendly and Bioactive Material from cellulose grafted soy protein isolate (SPI) for biomimetic calcium phosphate mineralization. Neat cellulose was oxidized using periodate then chemically modified by reacting with SPI followed by soaking in doubly concentrated simulated body fluid (2xSBF) solution. Scanning electron microscopy/energy dispersive X-ray spectroscopy, X-ray diffraction and transmission electron microscopy suggested the formation of uniform hydroxyapatite rod-like nanocrystals with ∼50 nm diameter. The chemical composition of the prepared Materials was further investigated using infrared spectroscopy and thermogravimetric analysis. The cytotoxicity of cellulose/SPI/calcium phosphate hybrid was evaluated using animal fibroblast baby hamster kidney cells (BHK-21). The cytotoxicity results suggested cellulose/SPI/calcium phosphate hybrid as potentially useful scaffold for regenerative therapies. The current article suggests a green strategy for preparing a new biohybrid Material for tissue engineering.