Bioartificial Organ - Explore the Science & Experts | ideXlab

Scan Science and Technology

Contact Leading Edge Experts & Companies

Bioartificial Organ

The Experts below are selected from a list of 117 Experts worldwide ranked by ideXlab platform

Ronald L Fournier – 1st expert on this subject based on the ideXlab platform

  • oxygen and inulin transport measurements in a planar tissue engineered Bioartificial Organ
    Tissue Engineering, 2002
    Co-Authors: Zhan Ding, Ronald L Fournier

    Abstract:

    In vivo oxygen and inulin transport rates were measured in a planar tissue-engineered Bioartificial Organ implanted in a rat. A compartmental model was used to describe the transport of oxygen and inulin between the cell chamber, across the immunoisolation membrane, and within the neovascularized region adjacent to the immunoisolation membrane. A nonlinear regression analysis of the plasma inulin levels and the oxygen transport rate into the device provided information on the degree of vascularization in the region adjacent to the Bioartificial Organ. Key parameters that were obtained from the analysis of the in vivo transport data included the average capillary blood oxygen partial pressure, the Krogh tissue cylinder radius, the extracellular volume fraction, and the capillary blood residence time. These four parameters are important indicators for assessing the degree of vascularization in the tissue adjacent to the immunoisolation membrane in the Bioartificial Organ. The oxygen and inulin transport tec…

  • in vivo measurement of solute transport rates in a Bioartificial Organ
    Tissue Engineering, 1999
    Co-Authors: Julie A Krohn, Ronald L Fournier, Andrew R Baker, Jennifer L Long, James P Byers

    Abstract:

    A radioactive tracer technique was used to evaluate the in vivo mass transfer properties of a tissue engineered Bioartificial Organ. To obtain these measurements, Bioartificial Organs were first im…

  • method for measuring in vivo oxygen transport rates in a Bioartificial Organ
    Tissue Engineering, 1999
    Co-Authors: David W Whalen, Zhan Ding, Ronald L Fournier

    Abstract:

    Oxygen transport is crucial for the proper functioning of a Bioartificial Organ. In many cases, the immunoisolation membrane used to protect the transplanted cells from the host’s immune system can be a significant barrier to oxygen transport. A method is described for measuring the in vitro and in vivo oxygen transport characteristics of a planar immunoisolation membrane. The in vitro oxygen permeability of the membrane was found to equal 9.22 × 10−4 cm/sec and was essentially the same as the in vivo value of 9.51 × 10−4 cm/sec. The fact that the in vitro and in vivo membrane permeabilities are identical indicates that any fibrotic tissue adjacent to the immunoisolation membrane did not present a significant resistance to the transport of oxygen. The measured oxygen permeability was also found consistent with the solute permeabilities obtained in a previous study for larger molecules. Based on the oxygen permeability results, theoretical calculations for this particular membrane indicate that about 1,100…

Norio Ohshima – 2nd expert on this subject based on the ideXlab platform

  • Packed-bed type reactor to attain high density culture of hepatocytes for use as a Bioartificial liver.
    Artificial Organs, 2008
    Co-Authors: Norio Ohshima, Kennichi Yanagi, Hirotoshi Miyoshi

    Abstract:

    : In an attempt to develop a Bioartificial liver using cultured hepatocytes, we investigated the short-term and long-term viability and metabolic functions of hepatocytes cultured in a new type of packed-bed type reactor using reticulated polyvinyl formal (PVF) resin as a supporting material. Perfusion culture experiments using this reactor, as well as monolayer cultures using conventional collagen-coated Petri dishes as control experiments, were performed. It was found that the highest density of immobilized hepatocytes attained with PVF resin was on the order of 107 cells/cm3 PVF and that hepatocytes cultured in this type of module for up to a week showed a sufficient level of liver-specific metabolic functions, such as ammonium metabolism, urea-N synthesis, and albumin secretion, to be comparable to those in the monolayer culture. It is concluded that the packed-bed reactor system utilizing PVF resin is a promising means to develop a Bioartificial Organ using hepatocytes.

  • A packed-bed reactor utilizing porous resin enables high density culture of hepatocytes
    Applied Microbiology and Biotechnology, 1992
    Co-Authors: Kennichi Yanagi, Hirotoshi Miyoshi, Hideki Fukuda, Norio Ohshima

    Abstract:

    To enable high density culture of hepatocytes for use as a hybrid artificial liver support system or a bioreactor system, a packed-bed reactor using collagen-coated reticulated polyvinyl formal (PVF) resin was applied to a primary culture of hepatocytes. Cubic PVF resins (2×2×2 mm, mean pore size: 100, 250 or 500 μm) were used as supporting substrates to immobilize hepatocytes. Two hundred and fifty cubes were packed in a cylindrical column, and 2.6–11.3×10^7 hepatocytes were seeded in the column by irrigating with 3 ml of the medium containing hepatocytes. Perfusion culture experiments using this packed-bed reactor, as well as monolayer cultures using conventional collagen-coated petri dishes as control experiments, were performed. Sufficient amounts of hepatocytes were found to be immobilized in the reticulated structure of the PVF resins. The highest density of immobilized hepatocytes attained with PVF resin was 1.2×10^7 cells/cm^3 PVF, which showed levels of ammonium removal and urea-N secretion comparable to those in the monolayer culture. It is concluded that the packed-bed reactor system utilizing PVF resin is a promising process for developing a bioreactor or a Bioartificial Organ using hepatocytes.

Zhan Ding – 3rd expert on this subject based on the ideXlab platform

  • oxygen and inulin transport measurements in a planar tissue engineered Bioartificial Organ
    Tissue Engineering, 2002
    Co-Authors: Zhan Ding, Ronald L Fournier

    Abstract:

    In vivo oxygen and inulin transport rates were measured in a planar tissue-engineered Bioartificial Organ implanted in a rat. A compartmental model was used to describe the transport of oxygen and inulin between the cell chamber, across the immunoisolation membrane, and within the neovascularized region adjacent to the immunoisolation membrane. A nonlinear regression analysis of the plasma inulin levels and the oxygen transport rate into the device provided information on the degree of vascularization in the region adjacent to the Bioartificial Organ. Key parameters that were obtained from the analysis of the in vivo transport data included the average capillary blood oxygen partial pressure, the Krogh tissue cylinder radius, the extracellular volume fraction, and the capillary blood residence time. These four parameters are important indicators for assessing the degree of vascularization in the tissue adjacent to the immunoisolation membrane in the Bioartificial Organ. The oxygen and inulin transport tec…

  • method for measuring in vivo oxygen transport rates in a Bioartificial Organ
    Tissue Engineering, 1999
    Co-Authors: David W Whalen, Zhan Ding, Ronald L Fournier

    Abstract:

    Oxygen transport is crucial for the proper functioning of a Bioartificial Organ. In many cases, the immunoisolation membrane used to protect the transplanted cells from the host’s immune system can be a significant barrier to oxygen transport. A method is described for measuring the in vitro and in vivo oxygen transport characteristics of a planar immunoisolation membrane. The in vitro oxygen permeability of the membrane was found to equal 9.22 × 10−4 cm/sec and was essentially the same as the in vivo value of 9.51 × 10−4 cm/sec. The fact that the in vitro and in vivo membrane permeabilities are identical indicates that any fibrotic tissue adjacent to the immunoisolation membrane did not present a significant resistance to the transport of oxygen. The measured oxygen permeability was also found consistent with the solute permeabilities obtained in a previous study for larger molecules. Based on the oxygen permeability results, theoretical calculations for this particular membrane indicate that about 1,100…