The Experts below are selected from a list of 1365 Experts worldwide ranked by ideXlab platform
Duujong Lee - One of the best experts on this subject based on the ideXlab platform.
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Biobutanol production from lignocellulosic biomass using immobilized clostridium acetobutylicum
Applied Energy, 2020Co-Authors: Tsung Yu Tsai, Jo Shu Chang, Dillirani Nagarajan, Duujong Lee, Cheng Di DongAbstract:Abstract Biobutanol produced by acetone-Biobutanol-ethanol (ABE) fermentation process has been revisited in the light of its use as “drop in” liquid biofuel to be blended with gasoline. In this study, renewable feedstock like rice straw, sugarcane bagasse and microalgal hydrolysate were used in ABE fermentation via separate hydrolysis and fermentation. Clostridium acetobutylicum ATCC 824 was used as the fermenting organism. Alkali pretreatment followed by enzymatic hydrolysis was used for rice straw and sugarcane bagasse. In batch fermentation, a Biobutanol titer and yield of 9.10 g/L and 0.42 mol/mol glucose (0.17 g Biobutanol/g glucose), respectively was obtained from rice straw, while sugarcane bagasse achieved a Biobutanol titer and yield of 8.40 g/L and 0.40 mol/mol glucose (0.16 g Biobutanol/g glucose), respectively. Higher microalgal biomass loading with 3% acid pretreatment severely inhibited fermentation performance. Unhydrolyzed microalgal biomass at a loading of 180 g/L in ABE fermentation resulted in 4.32 g/L Biobutanol and 0.09 g Biobutanol/g microalgae as yield. C. acetobutylicum was immobilized in polyvinyl alcohol (PVA) for improving the cell loading in fermentation and protect the cells from Biobutanol toxicity. With rice straw hydrolysate as a feedstock and in the absence of yeast extract, a Biobutanol titer, yield and productivity of 13.80 g/L, 0.90 g/L/h, and 0.58 mol Biobutanol/mol glucose (0.23 g Biobutanol/g glucose), respectively were obtained. Hence, rice straw is a potential feedstock for Biobutanol production for fuel use.
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current advances on fermentative Biobutanol production using third generation feedstock
Biotechnology Advances, 2017Co-Authors: Yue Wang, Hongwei Yen, Dillirani Nagarajan, Nanqi Ren, Duujong Lee, Akihiko Kondo, Jo Shu ChangAbstract:Biobutanol is gaining more attention as a potential alternative to ethanol, and the demand for fermentative Biobutanol production has renewed interest. The main challenge faced in Biobutanol production is the availability of feedstock. Using conventional agricultural biomass as feedstock is controversial and less efficient, while microalgae, the third generation feedstock, are considered promising feedstock for Biobutanol production due to their high growth rate and high carbohydrates content. This review is primarily focused on Biobutanol production by using carbohydrate-rich microalgal feedstock. Key technologies and challenges involved in producing butanol from microalgae are discussed in detail and future directions are also presented.
Jay P. Kesan - One of the best experts on this subject based on the ideXlab platform.
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A legal analysis of the effects of the Renewable Fuel Standard (RFS2) and Clean Air Act on the commercialization of Biobutanol as a transportation fuel in the United States
Gcb Bioenergy, 2012Co-Authors: Timothy A. Slating, Jay P. KesanAbstract:Biobutanol is currently a hot topic within discussions about second-generation biofuels. Its advocates point to the fact that it possesses a higher energy content than traditional bioethanol and, most importantly, that it is compatible with existing fuel distribution infrastructure. While traditional Biobutanol production processes have long since suffered from an inability to produce it in an economically viable manner, several recent technological advances have spurred interest from the private sector and several companies are now actively pursuing the commercialization of Biobutanol as a transportation fuel. As such, a legal analysis of the regulatory frameworks affecting this commercialization is highly relevant. In this study, we detail and analyze the two most import regulatory frameworks affecting the successful commercialization of Biobutanol as a transportation fuel in the United States. First, we provide a thorough description of the US Renewable Fuel Standard (RFS2) and analyze its impact on Biobutanol commercialization efforts. Next, we address the US Clean Air Act’s so-called ‘substantially similar’ prohibition and detail the three distinct regulatory paths it creates for Biobutanol commercialization. Finally, we conclude by exploring ways in which these regulatory frameworks could be altered to mitigate unjustified regulatory burdens. While our study focuses on the commercialization of Biobutanol, its regulatory descriptions and analysis are equally informative in regards to the commercialization of other alcohol-based biofuels.
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a legal analysis of the effects of the renewable fuel standard rfs2 and clean air act on the commercialization of Biobutanol as a transportation fuel in the united states
Social Science Research Network, 2011Co-Authors: Timothy A. Slating, Jay P. KesanAbstract:Biobutanol is currently a hot topic within discussions about second-generation biofuels. Its advocates point to the fact that it possesses a higher energy content than traditional bioethanol and, most importantly, that it is compatible with existing fuel distribution infrastructure. While traditional Biobutanol production processes have long since suffered from an inability to produce it in an economically viable manner, several recent technological advances have spurred interest from the private sector and several companies are now actively pursuing the commercialization of Biobutanol as a transportation fuel. As such, an analysis of the legal and regulatory frameworks affecting this commercialization is highly relevant. In this study, we detail and analyze the two most import regulatory frameworks affecting the successful commercialization of Biobutanol as a transportation fuel in the US. First, we provide a thorough description of the U.S. Renewable Fuel Standard (“RFS2”) and analyze its impact on Biobutanol commercialization efforts. Next, we address the U.S. Clean Air Act’s so-called “substantially similar” prohibition and detail the three distinct regulatory paths it creates for Biobutanol commercialization. Finally, we conclude by exploring ways in which these regulatory frameworks could be altered in order to mitigate unjustified regulatory burdens. While our study focuses on the commercialization of Biobutanol, its regulatory descriptions and analysis are equally informative in regards to the commercialization of other alcohol-based biofuels.
Mohamad Faizal Ibrahim - One of the best experts on this subject based on the ideXlab platform.
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Improved Biobutanol Production in 2-L Simultaneous Saccharification and Fermentation with Delayed Yeast Extract Feeding and in-situ Recovery
Scientific Reports, 2019Co-Authors: Muhammad Siddiq Mohamed Salleh, Mohamad Faizal Ibrahim, Ahmad Muhaimin Roslan, Suraini Abd-azizAbstract:Simultaneous saccharification and fermentation (SSF) with delayed yeast extract feeding (DYEF) was conducted in a 2-L bioreactor equipped with in-situ recovery using a gas stripping in order to enhance Biobutanol production from lignocellulosic biomass of oil palm empty fruit bunch (OPEFB). This study showed that 2.88 g/L of Biobutanol has been produced from SSF with a similar yield of 0.23 g/g as compared to separate hydrolysis and fermentation (SHF). An increase of 42% of Biobutanol concentration was observed when DYEF was introduced in the SSF at 39 h of fermentation operation. Biobutanol production was further enhanced up to 11% with a total improvement of 72% when in-situ recovery using a gas stripping was implemented to reduce the solvents inhibition in the bioreactor. In overall, DYEF and in-situ recovery were able to enhance Biobutanol production in SSF.
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advanced bioprocessing strategies for Biobutanol production from biomass
Renewable & Sustainable Energy Reviews, 2018Co-Authors: Mohamad Faizal Ibrahim, Seung Wook Kim, Suraini AbdazizAbstract:Abstract Biobutanol production as a renewable and sustainable biofuel is gaining interest in replacing non-renewable and depleting petrol fuel. In addition to its superior characteristics over bioethanol as a fuel in transportation, butanol is also in demand as a chemical substituent for various industries. Therefore, many researchers have investigated to produce Biobutanol at a low cost by considering suitable feedstock material and bioprocessing technologies. Renewable materials such as starch, lignocellulosic, and algal biomass are some of the common feedstock utilized for Biobutanol production, and each of them has its own advantages. The limitations of the conventional batch fermentation have been overcome by several fermentation operations and integrated bioprocessing technologies, which had improved Biobutanol production efficiency. The success of fermenting biomass into Biobutanol relies on the suitability of fermentation operation to correlate with the microbial behavior together with bioprocessing strategies in order to improve the whole process to be viable for industrial scale. Therefore, this review discusses the bioprocessing technologies and suitable strategies that have endeavored to enhance Biobutanol production from renewable biomass.
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Optimisation of Simultaneous Saccharification and Fermentation (SSF) for Biobutanol Production Using Pretreated Oil Palm Empty Fruit Bunch
MDPI AG, 2018Co-Authors: Nur Atheera Aiza Md Razali, Mohamad Faizal Ibrahim, Ezyana Kamal Bahrin, Suraini Abd-azizAbstract:This study was conducted in order to optimise simultaneous saccharification and fermentation (SSF) for Biobutanol production from a pretreated oil palm empty fruit bunch (OPEFB) by Clostridium acetobutylicum ATCC 824. Temperature, initial pH, cellulase loading and substrate concentration were screened using one factor at a time (OFAT) and further statistically optimised by central composite design (CCD) using the response surface methodology (RSM) approach. Approximately 2.47 g/L of Biobutanol concentration and 0.10 g/g of Biobutanol yield were obtained after being screened through OFAT with 29.55% increment (1.42 fold). The optimised conditions for SSF after CCD were: temperature of 35 °C, initial pH of 5.5, cellulase loading of 15 FPU/g-substrate and substrate concentration of 5% (w/v). This optimisation study resulted in 55.95% increment (2.14 fold) of Biobutanol concentration equivalent to 3.97 g/L and Biobutanol yield of 0.16 g/g. The model and optimisation design obtained from this study are important for further improvement of Biobutanol production, especially in consolidated bioprocessing technology
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cellulosic Biobutanol by clostridia challenges and improvements
Renewable & Sustainable Energy Reviews, 2017Co-Authors: Mohamad Faizal Ibrahim, Ezyana Kamal Bahrin, Norhayati Ramli, Suraini AbdazizAbstract:The gradual shift of transportation fuels from oil based fuels to alternative fuel resources and worldwide demand for energy has been the impetus for research to produce alcohol biofuels from renewable resources which focus on utilizing simple sugars from lignocellulosic biomass, the largest known renewable carbohydrate source as an alternative. Currently, the usage of bioethanol and biodiesel do not cover an increasing demand for biofuels. Hence, there is an extensive need for advanced biofuels with superior fuel properties. Biobutanol is regarded to be an excellent biofuel compared to bioethanol in terms of energy density and hygroscopicity, could be produced through acetone-butanol-ethanol (ABE) fermentation process. Even though the ABE fermentation is one of the oldest large-scale fermentation processes, Biobutanol yield by anaerobic fermentation remains sub-optimal. For sustainable industrial scale of Biobutanol production, a number of obstacles need to be addressed including choice of feedstock, low product yield, product toxicity to strain, multiple end-products and downstream processing of alcohol mixtures plus the metabolic engineering for improvement of fermentation process and products. Studies on the kinetic and physiological models for fermentation using lignocellulosic biomass provide useful information for process optimization. Simultaneous saccharification and fermentation (SSF) with in-situ product removal techniques have been developed to improve production economics due to the lower Biobutanol yield in the fermentation broth. The present review is attempting to provide an overall outlook on the discoveries and strategies that are being developed for Biobutanol production from lignocellulosic biomass.
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Statistical optimization of Biobutanol production from oil palm decanter cake hydrolysate by clostridium acetobutylicum ATCC 824
BioResources, 2013Co-Authors: Mohamad Nafis Abdul Razak, Phang Lai Yee, Mohamad Faizal Ibrahim, Mohd Ali Hassan, Suraini Abd-azizAbstract:Oil palm decanter cake (OPDC) is a potential lignocellulosic biomass for the biofuel industry. The fermentation conditions for Biobutanol production using glucose from OPDC hydrolysate by Clostridium acetobutylicum ATCC 824 were optimized via response surface methodology (RSM). An analysis of variance (ANOVA) using 2-level factorial was successfully screened. Three significant variables were found to influence the Biobutanol yield: glucose concentrations in the OPDC hydrolysate, inoculum sizes, and initial pH. The concentration of yeast extract, however, showed an insignificant effect in this study. The batch fermentation was analyzed using central composite design (CCD), and it yielded significant variables and the predicted optimum conditions were 70.00 g/L of OPDC hydrolysate, 16.20% of inoculum size, and an initial pH of 5.20. The predicted yield of Biobutanol was 0.09 g/g using 70.00 g/L of glucose. The optimum conditions were validated, and the actual Biobutanol yield was 0.11 g/g with 54.86 g/L of glucose consumption. The Biobutanol production using synthetic glucose was 15.38% higher when compared to OPDC hydrolysate, but the utilization of OPDC as alternative substrate was still comparable with other findings.
Timothy A. Slating - One of the best experts on this subject based on the ideXlab platform.
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A legal analysis of the effects of the Renewable Fuel Standard (RFS2) and Clean Air Act on the commercialization of Biobutanol as a transportation fuel in the United States
Gcb Bioenergy, 2012Co-Authors: Timothy A. Slating, Jay P. KesanAbstract:Biobutanol is currently a hot topic within discussions about second-generation biofuels. Its advocates point to the fact that it possesses a higher energy content than traditional bioethanol and, most importantly, that it is compatible with existing fuel distribution infrastructure. While traditional Biobutanol production processes have long since suffered from an inability to produce it in an economically viable manner, several recent technological advances have spurred interest from the private sector and several companies are now actively pursuing the commercialization of Biobutanol as a transportation fuel. As such, a legal analysis of the regulatory frameworks affecting this commercialization is highly relevant. In this study, we detail and analyze the two most import regulatory frameworks affecting the successful commercialization of Biobutanol as a transportation fuel in the United States. First, we provide a thorough description of the US Renewable Fuel Standard (RFS2) and analyze its impact on Biobutanol commercialization efforts. Next, we address the US Clean Air Act’s so-called ‘substantially similar’ prohibition and detail the three distinct regulatory paths it creates for Biobutanol commercialization. Finally, we conclude by exploring ways in which these regulatory frameworks could be altered to mitigate unjustified regulatory burdens. While our study focuses on the commercialization of Biobutanol, its regulatory descriptions and analysis are equally informative in regards to the commercialization of other alcohol-based biofuels.
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a legal analysis of the effects of the renewable fuel standard rfs2 and clean air act on the commercialization of Biobutanol as a transportation fuel in the united states
Social Science Research Network, 2011Co-Authors: Timothy A. Slating, Jay P. KesanAbstract:Biobutanol is currently a hot topic within discussions about second-generation biofuels. Its advocates point to the fact that it possesses a higher energy content than traditional bioethanol and, most importantly, that it is compatible with existing fuel distribution infrastructure. While traditional Biobutanol production processes have long since suffered from an inability to produce it in an economically viable manner, several recent technological advances have spurred interest from the private sector and several companies are now actively pursuing the commercialization of Biobutanol as a transportation fuel. As such, an analysis of the legal and regulatory frameworks affecting this commercialization is highly relevant. In this study, we detail and analyze the two most import regulatory frameworks affecting the successful commercialization of Biobutanol as a transportation fuel in the US. First, we provide a thorough description of the U.S. Renewable Fuel Standard (“RFS2”) and analyze its impact on Biobutanol commercialization efforts. Next, we address the U.S. Clean Air Act’s so-called “substantially similar” prohibition and detail the three distinct regulatory paths it creates for Biobutanol commercialization. Finally, we conclude by exploring ways in which these regulatory frameworks could be altered in order to mitigate unjustified regulatory burdens. While our study focuses on the commercialization of Biobutanol, its regulatory descriptions and analysis are equally informative in regards to the commercialization of other alcohol-based biofuels.
Aidil Abdul Hamid - One of the best experts on this subject based on the ideXlab platform.
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Isolation of a Clostridium acetobutylicum strain and characterization of its fermentation performance on agricultural wastes
Renewable Energy, 2016Co-Authors: Najeeb Kaid Nasser Al-shorgani, Mohamed Hasnain Isa, Mohd Sahaid Kalil, Wan Mohtar Wan Yusoff, Aidil Abdul HamidAbstract:A new solvent-producing Clostridium has been isolated from soil used in intensive rice cultivation. The 16S rRNA analysis of the isolate indicates that it is closely related to Clostridium acetobutylicum, with a sequence identity of 96%. The new isolate, named C. acetobutylicum YM1, produces Biobutanol from multiple carbon sources, including glucose, fructose, xylose, arabinose, glycerol, lactose, cellobiose, mannitol, maltose, galactose, sucrose and mannose. This isolate can also utilize polysaccharides such as starch and carboxylmethyl cellulose (CMC) for the production of Biobutanol. The ability of isolate YM1 to produce Biobutanol from agro-industrial wastes was also evaluated for rice bran, de-oiled rice bran, palm oil mill effluent and palm kernel cake. The highest concentration of Biobutanol (7.27 g/L) was obtained from the fermentation medium containing 2% (w/v) fructose, with a total acetone-butanol-ethanol (ABE) concentration of 10.23 g/L. The ability of isolate YM1 to produce Biobutanol from various carbon sources and agro-wastes indicates the promise of the use of this isolate for the production of Biobutanol, a renewable energy resource, from readily available renewable feedstocks.
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Biobutanol production by a new aerotolerant strain of clostridium acetobutylicum ym1 under aerobic conditions
Fuel, 2015Co-Authors: Najeeb Kaid Nasser Alshorgani, Mohd Sahaid Kalil, Wan Mohtar Wan Yusoff, Aidil Abdul HamidAbstract:Abstract A newly isolated strain of Clostridium acetobutylicum YM1 has a unique property of producing Biobutanol from glucose under aerobic conditions. This strain exhibited the capability to grow and produce high concentrations of Biobutanol under different concentrations of dissolved oxygen (DO). The growth profile and solvent production in a 5 L bioreactor were similar under anaerobic and aerobic conditions (100% initial DO saturation), and the final Biobutanol production was 12.18 and 12.30 g/L, respectively. The addition of reducing chemical agents, without creating anaerobic conditions, to the culture of YM1 enhanced the production of Biobutanol. Strain YM1 possesses different enzymes that are responsible for oxygen scavenging, such as superoxide dismutase (SOD), catalase and NADH/NADPH oxidases. This study provides a simple operation strategy for more efficient Biobutanol production by using an aerotolerant strain of C. acetobutylicum YM1 without the need to flush the medium with nitrogen gas to ensure anaerobic conditions.
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Biobutanol production from palm kernel cake pkc using clostridium saccharoperbutylacetonicum n1 4 in batch culture fermentation
Bioresources, 2014Co-Authors: Hafiza Shukor, Najeeb Kaid Nasser Alshorgani, Nurina Anuar, Peyman Abdeshahian, Aidil Abdul Hamid, Norliza Abd. Rahman, Mohd Hafez Mohd Isa, Mohd Sahaid KalilAbstract:Palm kernel cake (PKC), a by-product of palm oil industry, contains glucose and mannose as hexose sugars. This study was performed to determine the feasibility of using PKC as a lignocellulosic substrate for Biobutanol production by Clostridium saccharoperbutylacetonicum N1-4 in an acetone-butanol-ethanol (ABE) fermentation process. Moreover, the effect of tryptone-yeast extract-acetate (TYA) medium and P2 medium on Biobutanol production was evaluated. Experimental results showed that butanol production of 3.05 g/L was obtained using mannose sugar, which was comparable to 3.61 g/L butanol production measured using glucose. Moreover, the maximum production of Biobutanol (0.38 g/L) was obtained at a PKC concentration of 30%, indicating the possibility of PKC utilization in butanol production. ABE fermentation of PKC using distilled water, TYA medium, and P2 medium showed that the highest butanol production (0.26 g/L) with ABE production of 0.38 g/L was obtained when ABE fermentation was conducted in P2 medium.
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enhanced butanol production by clostridium acetobutylicum ncimb 13357 grown on date fruit as carbon source in p2 medium
The Scientific World Journal, 2014Co-Authors: Emran I Khamaiseh, Peyman Abdeshahian, Wan Mohtar Wan Yusoff, Aidil Abdul Hamid, Mohd Sahaid KalilAbstract:The production of Biobutanol was studied by the cultivation of Clostridium acetobutylicum NCIMB 13557 in P2 medium including date fruit as the sole substrate. The effect of P2 medium and the effect of different concentrations of date fruit ranging from 10 to 100 g/L on Biobutanol production were investigated. Anaerobic batch culture was carried out at 35°C incubation temperature and pH 7.0 ± 0.2 for 72 h. Experimental results showed that the lowest yield of Biobutanol and acetone-butanol-ethanol (ABE) was 0.32 and 0.35 gram per gram of carbohydrate consumed (g/g), respectively, when an initial date fruit concentration of 10 g/L was utilized. At this fruit date concentration a Biobutanol production value of 1.56 g/L was obtained. On the other hand, the maximum yield of Biobutanol (0.48 g/g) and ABE (0.63 g/g) was produced at 50 g/L date fruit concentration with a Biobutanol production value as high as 11 g/L. However, when a higher initial date fruit concentration was used, Biobutanol and ABE production decreased to reach the yield of 0.22 g/g and 0.35 g/g, respectively, where 100 g/L date fruit was used. Similar results also revealed that 10.03 g/L Biobutanol was produced using 100 g/L date fruit.
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pre optimization of medium for Biobutanol production by a new isolate of solvent producing clostridium
Bioresources, 2013Co-Authors: Najeeb Kaid Nasser Alshorgani, Wan Mohtar Wan Yusoff, Aidil Abdul Hamid, Mohd Sahaid KalilAbstract:A Plackett-Burman design was used to pre-optimize the medium composition for Biobutanol production using a unique isolate of solvent-producing Clostridium YM1. Various nutrient factors affecting Biobutanol production were screened using the Plackett-Burman design. These factors included: glucose, tryptone, yeast extract, peptone, ammonium acetate, KH2PO4, K2HPO4, MgSO4, FeSO4, Na2CO3, and NaCl. The results were analyzed by an analysis of variance (ANOVA), which showed that glucose, tryptone, yeast extract, peptone, K2HPO4, Na2CO3, and MgSO4 had significant effects on Biobutanol production. However, ammonium acetate, KH2PO4, and FeSO4 had insignificant effects. The established model from the ANOVA analysis had a significant value of Pmodel>F = 0.0245 and an R2 value of 0.999. The estimated maximum Biobutanol production was 9.01 g/L, whereas the optimized medium produced 10.93 g/L of Biobutanol.