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Marcio A. Mazutti - One of the best experts on this subject based on the ideXlab platform.
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New perspectives for weeds control using autochthonous fungi with selective Bioherbicide potential.
Heliyon, 2019Co-Authors: Francisco Wilson Reichert Júnior, Maurício Albertoni Scariot, César Tiago Forte, Leonardo Pandolfi, Jaqueline Mara Dil, Sabrina Natalia Weirich, Carine Carezia, Jéssica Mulinari, Marcio A. Mazutti, Gislaine FongaroAbstract:Abstract The prospection of Bioherbicides has been an alternative to weed control, aiming at mitigating chemical risks to human, animal and environmental health due to extreme use of synthetic herbicides. In the present study, various fungi were isolated from plants with symptoms of fungal diseases for Bioherbicide purposes against weeds (Urochloa plantaginea, Euphorbia heterophylla and Bidens pilosa). Fungi isolated were identified by molecular methods and enzymatic products obtained by fungi fermentation (cellulase, lipase, peroxidase, and amylase) were quantified. Bioherbicide selectivity study was performed on crops (soybean and corn), as well as on resistant weeds. Among the isolated fungi, Fusarium oxysporum, Fusarium ploriferatum, and Trichoderma koningiopsis presented Bioherbicide potential. T. koningiopsis, in particular, presented the highest effect on Euphorbia heterophylla (popular name - Mexican fire plant), causing up to 60% of foliar damage, without presenting phytotoxicity against corn crop. New perspectives for weeds control and their use in corn crops were prospected, considering the Bioherbicide selectivity described in this study.
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Production of cutinase by solid-state fermentation and its use as adjuvant in Bioherbicide formulation
Bioprocess and biosystems engineering, 2019Co-Authors: Caroline Torres De Oliveira, Eliana A. Alves, Izelmar Todero, Raquel C. Kuhn, Débora De Oliveira, Marcio A. MazuttiAbstract:In the present study, it was presented a strategy to maximize the cutinase production by solid-state fermentation from different microorganisms and substrates. The best results were observed using Fusarium verticillioides, rice bran being the main substrate. Maximum yield of cutinase obtained by the strain was 16.22 U/g. For concentration, ethanol precipitation was used, and the purification factor was 2.4. The optimum temperature and pH for enzyme activity were 35 °C and 6.5, respectively. The enzyme was stable at a wide range of temperature and at all pH values tested. The concentrated cutinase was used as an adjuvant in a formulation containing cutinase + Bioherbicide. The use of enzyme increased the efficiency of Bioherbicide, since cutinase was responsible to remove/degrade the cutin that recovery the weed leaves and difficult the Bioherbicide absorption. Cutinase showed to be a promising product to be used in formulation of Bioherbicides.
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Solid-state fermentation for production of a Bioherbicide from Diaporthe sp. and its formulation to enhance the efficacy.
3 Biotech, 2017Co-Authors: Bruna De Oliveira Bastos, Raquel C. Kuhn, Thiarles Brun, Gabriel Antônio Deobald, Valéria Dal Prá, Emanuele Junges, Aniela Kempka Pinto, Marcio A. MazuttiAbstract:In this study, a Bioherbicide was produced by solid-state fermentation (SSF) using Diaporthe sp. Adjuvants were employed in a formulation to enhance the herbicidal activity towards the target (Cucumis sativus). The study was divided into two steps: (1) the fermentation condition for Bioherbicide production was assessed; (2) evaluation of different formulations containing palm oil, Tween® 80 and Span® 80, in order to increase phytotoxicity. In step 1, the maximum herbicidal activity (1.23% of the leaves had lesions) was obtained at 25 °C, moisture content of 50 wt%, supplemented with 10 wt% of corn steep liquor and soybean bran and inoculum density of 15 wt%. In step 2, the formulation containing 8.2 wt% of palm oil, 8.2 wt% of Tween® 80 and Span® 80, resulting in an HLB of 12.8 showed the highest phytotoxicity on the leaves. At this condition, dry matter and height of target were reduced about 36% in comparison with control. Diaporthe sp. has the potential to produce molecules with herbicidal activity and the use of adjuvants enhanced three times its efficiency.
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Optimization of solid-state fermentation for Bioherbicide production by Phoma sp .
Brazilian Journal of Chemical Engineering, 2017Co-Authors: Rodrigo Klaic, Raquel C. Kuhn, Jerson Vanderlei Carús Guedes, Daniela Sallet, Edson Luiz Foletto, Rodrigo Josemar Seminoti Jacques, Marcio A. MazuttiAbstract:Abstract In this work the Bioherbicide production from Phoma sp. by solid-state fermentation was optimized. Agroindustrial residues such as bagasse, soybean bran and corn steep liquor were used as substrate. The Bioherbicide was extracted from the fermented solid and the supernatant was applied for the control of the target plant. The evaluated responses in the bioassay were plant height, root length, fresh and dry weight, number of flowers and phytotoxicity. The results in the bioassays demonstrated that the Bioherbicide presented activity towards the target plant and the intensity of the effect was influenced by the formulation of the fermentation medium. The optimized condition for Bioherbicide production was (wt%): moisture content 70.0, soybean bran content 30.0 and corn steep liquor (CSL) 20.0, it being possible to obtain an injury phytotoxicity level of 40. The Bioherbicide showed a mode of action based on the inhibition of carotenoid biosynthesis
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Bioherbicide based on Diaporthe sp. secondary metabolites in the control of three tough weeds
African Journal of Agricultural Research, 2016Co-Authors: Maiquel Pizzuti Pes, Marcio A. Mazutti, Thiago Castro De Almeida, Luis Eduardo Curioletti, Adriano Arrué Melo, Jerson Vanderlei Carús Guedes, Raquel C. KuhnAbstract:Brazilian growers are facing difficulties to manage the weeds Lolium multiflorum, Conyza sp. and Echinochloa sp. using commercially available agrochemicals in most crops. This study aims to evaluate the use of a Bioherbicide based on fermented broth containing secondary metabolites of Diaporthe sp. to control L. multiflorum, Conyza sp. and Echinochloa sp. The Bioherbicide activity in pre-emergence and post-emergence of these weed species and phytotoxicity on soybean, wheat and rice plants were evaluated. In the pre-emergence test using all weed species, the Bioherbicide showed 100% control efficiency in comparison with the control group. Phytotoxic symptoms were observed on soybean leaves and horseweed was efficiently controlled using the Bioherbicide. Key words: Bioherbicide, weeds, bioproducts.
Raquel C. Kuhn - One of the best experts on this subject based on the ideXlab platform.
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Development of a Solid Bioherbicide Formulation by Spray Drying Technology
Agriculture, 2020Co-Authors: Thiago Castro De Almeida, Stefani S. Spannemberg, Thiarles Brun, Silvana Schmaltz, Otávio Dos Santos Escobar, Danie Martini Sanchotene, Sylvio Henrique Bidel Dornelles, Giovani L. Zabot, Marcus V. Tres, Raquel C. KuhnAbstract:This study aimed to compare the herbicidal activity of solid formulas obtained by spray drying with conventional liquid formulas containing biomolecules produced by submerged cultivation of the fungus Diaporthe sp. in a stirred-tank bioreactor. The solid formula presented the highest phytotoxicity on plant control (96.7%) and the phytotoxicity was directly related to the concentration of fermented broth in the formula. The use of adjuvant improved the efficiency of the Bioherbicide. Dry matters of treatments were lower than the control and this was correlated with an increase in oxidative stress, since the activity of the antioxidant enzymes such as superoxide dismutase and guaiacol peroxidase increased in the treatment with a high level of phytotoxicity. Spray drying technology is a promising tool to concentrate Bioherbicide without the loss of bioactive compounds since one of the major challenges in the production of Bioherbicides is the low concentration of active ingredients in the fermented broth.
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Production of cutinase by solid-state fermentation and its use as adjuvant in Bioherbicide formulation
Bioprocess and biosystems engineering, 2019Co-Authors: Caroline Torres De Oliveira, Eliana A. Alves, Izelmar Todero, Raquel C. Kuhn, Débora De Oliveira, Marcio A. MazuttiAbstract:In the present study, it was presented a strategy to maximize the cutinase production by solid-state fermentation from different microorganisms and substrates. The best results were observed using Fusarium verticillioides, rice bran being the main substrate. Maximum yield of cutinase obtained by the strain was 16.22 U/g. For concentration, ethanol precipitation was used, and the purification factor was 2.4. The optimum temperature and pH for enzyme activity were 35 °C and 6.5, respectively. The enzyme was stable at a wide range of temperature and at all pH values tested. The concentrated cutinase was used as an adjuvant in a formulation containing cutinase + Bioherbicide. The use of enzyme increased the efficiency of Bioherbicide, since cutinase was responsible to remove/degrade the cutin that recovery the weed leaves and difficult the Bioherbicide absorption. Cutinase showed to be a promising product to be used in formulation of Bioherbicides.
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Solid-state fermentation for production of a Bioherbicide from Diaporthe sp. and its formulation to enhance the efficacy.
3 Biotech, 2017Co-Authors: Bruna De Oliveira Bastos, Raquel C. Kuhn, Thiarles Brun, Gabriel Antônio Deobald, Valéria Dal Prá, Emanuele Junges, Aniela Kempka Pinto, Marcio A. MazuttiAbstract:In this study, a Bioherbicide was produced by solid-state fermentation (SSF) using Diaporthe sp. Adjuvants were employed in a formulation to enhance the herbicidal activity towards the target (Cucumis sativus). The study was divided into two steps: (1) the fermentation condition for Bioherbicide production was assessed; (2) evaluation of different formulations containing palm oil, Tween® 80 and Span® 80, in order to increase phytotoxicity. In step 1, the maximum herbicidal activity (1.23% of the leaves had lesions) was obtained at 25 °C, moisture content of 50 wt%, supplemented with 10 wt% of corn steep liquor and soybean bran and inoculum density of 15 wt%. In step 2, the formulation containing 8.2 wt% of palm oil, 8.2 wt% of Tween® 80 and Span® 80, resulting in an HLB of 12.8 showed the highest phytotoxicity on the leaves. At this condition, dry matter and height of target were reduced about 36% in comparison with control. Diaporthe sp. has the potential to produce molecules with herbicidal activity and the use of adjuvants enhanced three times its efficiency.
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Optimization of solid-state fermentation for Bioherbicide production by Phoma sp .
Brazilian Journal of Chemical Engineering, 2017Co-Authors: Rodrigo Klaic, Raquel C. Kuhn, Jerson Vanderlei Carús Guedes, Daniela Sallet, Edson Luiz Foletto, Rodrigo Josemar Seminoti Jacques, Marcio A. MazuttiAbstract:Abstract In this work the Bioherbicide production from Phoma sp. by solid-state fermentation was optimized. Agroindustrial residues such as bagasse, soybean bran and corn steep liquor were used as substrate. The Bioherbicide was extracted from the fermented solid and the supernatant was applied for the control of the target plant. The evaluated responses in the bioassay were plant height, root length, fresh and dry weight, number of flowers and phytotoxicity. The results in the bioassays demonstrated that the Bioherbicide presented activity towards the target plant and the intensity of the effect was influenced by the formulation of the fermentation medium. The optimized condition for Bioherbicide production was (wt%): moisture content 70.0, soybean bran content 30.0 and corn steep liquor (CSL) 20.0, it being possible to obtain an injury phytotoxicity level of 40. The Bioherbicide showed a mode of action based on the inhibition of carotenoid biosynthesis
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Production of Bioherbicide by Phoma sp. in a stirred-tank bioreactor
3 Biotech, 2016Co-Authors: Thiarles Brun, Izelmar Todero, Raquel C. Kuhn, Thiago Castro De Almeida, J. E. Rabuske, Jair João Daniel Junior, Gustavo Ariotti, Tássia C. Confortin, Jonas André Arnemann, Jerson Vanderlei Carús GuedesAbstract:The objective of this work was to produce an herbicide by submerged fermentation in a stirred-tank bioreactor and to assess the potential herbicidal in pre-emergence, post-emergence, and in a detached leaves of Cucumis sativus var species. wisconsin (cucumber) and Sorghum bicolor (sorghum) species. Fermentations were carried out in a stirred-tank bioreactor with useful volume of 3L. Stirring rate (40, 50, and 60 rpm) and aeration (1, 2 and 3 vvm) were the variables studied for Bioherbicide production. Fermented broth was fractioned with different solvents to identify the molecules produced by the fungus in a multi-dimensional gas chromatograph system. Bioherbicide showed 100% inhibition of germination of both species in the pre-emergence tests. From detached leaves tests were verified yellowish lesions in Cucumis sativus and necrotic lesions on leaves of Sorghum bicolor. Post-emergence test presented variation of the phytotoxicity from 25 to 66% for the species C. sativus and from 32 to 58% by S. bicolor. The metabolites produced by submerged fermentation of Phoma sp. presented activity in pre-emergence, post-emergence, and detached leaves of C. sativus and S. bicolor and it could be an alternative in the future for weed control.
Jerson Vanderlei Carús Guedes - One of the best experts on this subject based on the ideXlab platform.
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Optimization of solid-state fermentation for Bioherbicide production by Phoma sp .
Brazilian Journal of Chemical Engineering, 2017Co-Authors: Rodrigo Klaic, Raquel C. Kuhn, Jerson Vanderlei Carús Guedes, Daniela Sallet, Edson Luiz Foletto, Rodrigo Josemar Seminoti Jacques, Marcio A. MazuttiAbstract:Abstract In this work the Bioherbicide production from Phoma sp. by solid-state fermentation was optimized. Agroindustrial residues such as bagasse, soybean bran and corn steep liquor were used as substrate. The Bioherbicide was extracted from the fermented solid and the supernatant was applied for the control of the target plant. The evaluated responses in the bioassay were plant height, root length, fresh and dry weight, number of flowers and phytotoxicity. The results in the bioassays demonstrated that the Bioherbicide presented activity towards the target plant and the intensity of the effect was influenced by the formulation of the fermentation medium. The optimized condition for Bioherbicide production was (wt%): moisture content 70.0, soybean bran content 30.0 and corn steep liquor (CSL) 20.0, it being possible to obtain an injury phytotoxicity level of 40. The Bioherbicide showed a mode of action based on the inhibition of carotenoid biosynthesis
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Production of Bioherbicide by Phoma sp. in a stirred-tank bioreactor
3 Biotech, 2016Co-Authors: Thiarles Brun, Izelmar Todero, Raquel C. Kuhn, Thiago Castro De Almeida, J. E. Rabuske, Jair João Daniel Junior, Gustavo Ariotti, Tássia C. Confortin, Jonas André Arnemann, Jerson Vanderlei Carús GuedesAbstract:The objective of this work was to produce an herbicide by submerged fermentation in a stirred-tank bioreactor and to assess the potential herbicidal in pre-emergence, post-emergence, and in a detached leaves of Cucumis sativus var species. wisconsin (cucumber) and Sorghum bicolor (sorghum) species. Fermentations were carried out in a stirred-tank bioreactor with useful volume of 3L. Stirring rate (40, 50, and 60 rpm) and aeration (1, 2 and 3 vvm) were the variables studied for Bioherbicide production. Fermented broth was fractioned with different solvents to identify the molecules produced by the fungus in a multi-dimensional gas chromatograph system. Bioherbicide showed 100% inhibition of germination of both species in the pre-emergence tests. From detached leaves tests were verified yellowish lesions in Cucumis sativus and necrotic lesions on leaves of Sorghum bicolor. Post-emergence test presented variation of the phytotoxicity from 25 to 66% for the species C. sativus and from 32 to 58% by S. bicolor. The metabolites produced by submerged fermentation of Phoma sp. presented activity in pre-emergence, post-emergence, and detached leaves of C. sativus and S. bicolor and it could be an alternative in the future for weed control.
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Bioherbicide based on Diaporthe sp. secondary metabolites in the control of three tough weeds
African Journal of Agricultural Research, 2016Co-Authors: Maiquel Pizzuti Pes, Marcio A. Mazutti, Thiago Castro De Almeida, Luis Eduardo Curioletti, Adriano Arrué Melo, Jerson Vanderlei Carús Guedes, Raquel C. KuhnAbstract:Brazilian growers are facing difficulties to manage the weeds Lolium multiflorum, Conyza sp. and Echinochloa sp. using commercially available agrochemicals in most crops. This study aims to evaluate the use of a Bioherbicide based on fermented broth containing secondary metabolites of Diaporthe sp. to control L. multiflorum, Conyza sp. and Echinochloa sp. The Bioherbicide activity in pre-emergence and post-emergence of these weed species and phytotoxicity on soybean, wheat and rice plants were evaluated. In the pre-emergence test using all weed species, the Bioherbicide showed 100% control efficiency in comparison with the control group. Phytotoxic symptoms were observed on soybean leaves and horseweed was efficiently controlled using the Bioherbicide. Key words: Bioherbicide, weeds, bioproducts.
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Bioherbicide production by Diaporthe sp. isolated from the Brazilian Pampa biome
Biocatalysis and Agricultural Biotechnology, 2015Co-Authors: Angélica Rossana Castro De Souza, Raquel C. Kuhn, Jerson Vanderlei Carús Guedes, Rodrigo Josemar Seminoti Jacques, Daiana Bortoluzzi Baldoni, Jessica Lima, Vitória Porto, Camila Marcuz, Rafael Camargo Ferraz, Marcio A. MazuttiAbstract:Abstract In this study was presented a rational strategy to scale-up a wild fungal strain from the Brazilian Pampa biome to produce compounds with herbicidal activity in pre-emergence. It was defined the medium composition for the growth and production of Bioherbicide in shaken flasks using sucrose corn steep liquor as substrate. In a second step, the process variables in a stirred-tank bioreactor were assessed. Maximum biomass production in shaken flasks was 23.87 g L −1 (41% higher than synthetic media) and in the bioreator 27.8 g L −1 . The Diaporthe sp. growth in bioreactor was negatively affected by agitation higher than 40 rpm. The Bioherbicide efficiency rate reached 100%, since none seed germinated after the application of fermented broth. The Brazilian Pampa biome could be an effective way to obtain high-value products from the biodiversity.
Lise Barthelmebs - One of the best experts on this subject based on the ideXlab platform.
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Impact of Leptospermone, a Natural β-Triketone Herbicide, on the Fungal Composition and Diversity of Two Arable Soils
Frontiers in Microbiology, 2019Co-Authors: Clarisse Mallet, Sana Romdhane, Christophe Calvayrac, Fabrice Martin-laurent, Camille Loiseau, Jérémie Béguet, Lise BarthelmebsAbstract:Impact of leptospermone, a β-triketone Bioherbicide, was investigated on the fungal community which supports important soil ecological functions such as decomposition of organic matter and nutrients recycling. This study was done in a microcosm experiment using two French soils, Perpignan (P) and Saint-Jean-de-Fos (SJF), differing in their physicochemical properties and history treatment with synthetic β-triketones. Soil microcosms were treated with leptospermone at recommended dose and incubated under controlled conditions for 45 days. Untreated microcosms were used as control. Illumina MiSeq sequencing of the internal transcribed spacer region of the fungal rRNA revealed significant changes in fungal community structure and diversity in both soils. Xylariales, Hypocreales, Pleosporales and Capnodiales (Ascomycota phyla) fungi and those belonging to Sebacinales, Cantharellales, Agaricales, Polyporales, Filobasidiales and Tremellales orders (Basidiomycota phyla) were well represented in treated soil microcosms compared to control. Nevertheless, while for the treated SJF a complete recovery of the fungal community was observed at the end of the experiment, this was not the case for the P treated soil, although no more Bioherbicide remained. Indeed, the relative abundance of most of the saprophytic fungi were lower in treated soil compared to control microcosms whereas fungi from parasitic fungi included in Spizellomycetales and Pezizales orders increased. To the best of our knowledge, this is the only study assessing the effect of the Bioherbicide leptospermone on the composition and diversity of the fungal community in soil. This study showed that leptospermone has an impact on α- and β-diversity of the fungal community. It underlines the possible interest of microbial endpoints for environmental risk assessment of biopesticide.
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Table_1_Impact of Leptospermone, a Natural β-Triketone Herbicide, on the Fungal Composition and Diversity of Two Arable Soils.XLSX
2019Co-Authors: Clarisse Mallet, Sana Romdhane, Christophe Calvayrac, Fabrice Martin-laurent, Camille Loiseau, Jérémie Béguet, Lise BarthelmebsAbstract:Impact of leptospermone, a β-triketone Bioherbicide, was investigated on the fungal community which supports important soil ecological functions such as decomposition of organic matter and nutrients recycling. This study was done in a microcosm experiment using two French soils, Perpignan (P) and Saint-Jean-de-Fos (SJF), differing in their physicochemical properties and history treatment with synthetic β-triketones. Soil microcosms were treated with leptospermone at recommended dose and incubated under controlled conditions for 45 days. Untreated microcosms were used as control. Illumina MiSeq sequencing of the internal transcribed spacer region of the fungal rRNA revealed significant changes in fungal community structure and diversity in both soils. Xylariales, Hypocreales, Pleosporales and Capnodiales (Ascomycota phyla) fungi and those belonging to Sebacinales, Cantharellales, Agaricales, Polyporales, Filobasidiales and Tremellales orders (Basidiomycota phyla) were well represented in treated soil microcosms compared to control. Nevertheless, while for the treated SJF a complete recovery of the fungal community was observed at the end of the experiment, this was not the case for the P treated soil, although no more Bioherbicide remained. Indeed, the relative abundance of most of the saprophytic fungi were lower in treated soil compared to control microcosms whereas fungi from parasitic fungi included in Spizellomycetales and Pezizales orders increased. To the best of our knowledge, this is the only study assessing the effect of the Bioherbicide leptospermone on the composition and diversity of the fungal community in soil. This study showed that leptospermone has an impact on α- and β-diversity of the fungal community. It underlines the possible interest of microbial endpoints for environmental risk assessment of biopesticide.
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Devenir, biodégradation et impact écotoxicologique du Bioherbicide leptospermone dans les sols
2017Co-Authors: Sana Romdhane, Franck E. Dayan, Marion Devers-lamrani, Lise Barthelmebs, Christophe Calvayrac, Cédric Bertrand, Jean-françois Cooper, Fabrice Martin-laurentAbstract:Les herbicides sont appliqués dans le monde entier pour contrôler le développement et la propagation des plantes adventices afin d’assurer le rendement et la qualité des productions végétales. Toutefois, en raison de leur utilisation généralisée, des résidus d’herbicides persistent dans les sols agricoles d’où ils peuvent être transférés et contribués à la contamination de différents compartiments de l’environnement. L’utilisation de composés d’origine naturelle ayant une activité herbicide (Bioherbicide) est vue comme une alternative respectueuse de l’environnement pour la protection des cultures. Toutefois, malgré ce profil environnemental favorable, le devenir et l’impact écotoxicologique des Bioherbicides dans les sols ne sont pas connus. Dans ce contexte, nous avons étudié le devenir, la biodégradation et l’impact écotoxicologique du Bioherbicide leptospermone sur la communauté bactérienne des sols. La dissipation de la leptospermone a été caractérisée dans deux sols agricoles. Elle est sous le contrôle de processus abiotique (photolyse) et biotique (biodégradation). Methylophilus sp. LS1, une souche microbienne dégradante, a été isolée et caractérisée permettant d’identifier des produits de transformation, dont l’hydroxyleptospermone, et de proposer une voie de transformation de la leptospermone (Romdhane et al., 2017). L’impact écotoxicologique de la leptospermone sur la composition et la diversité α- et β- de la communauté bactérienne a été évalué. Nous avons montré que la communauté bactérienne des deux sols agricoles était sensible à la leptospermone. L’impact écotoxicologique était transitoire et la communauté bactérienne était résiliente dans le sol ou la dissipation de la leptospermone
Fabrice Martin-laurent - One of the best experts on this subject based on the ideXlab platform.
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Impact of Leptospermone, a Natural β-Triketone Herbicide, on the Fungal Composition and Diversity of Two Arable Soils
Frontiers in Microbiology, 2019Co-Authors: Clarisse Mallet, Sana Romdhane, Christophe Calvayrac, Fabrice Martin-laurent, Camille Loiseau, Jérémie Béguet, Lise BarthelmebsAbstract:Impact of leptospermone, a β-triketone Bioherbicide, was investigated on the fungal community which supports important soil ecological functions such as decomposition of organic matter and nutrients recycling. This study was done in a microcosm experiment using two French soils, Perpignan (P) and Saint-Jean-de-Fos (SJF), differing in their physicochemical properties and history treatment with synthetic β-triketones. Soil microcosms were treated with leptospermone at recommended dose and incubated under controlled conditions for 45 days. Untreated microcosms were used as control. Illumina MiSeq sequencing of the internal transcribed spacer region of the fungal rRNA revealed significant changes in fungal community structure and diversity in both soils. Xylariales, Hypocreales, Pleosporales and Capnodiales (Ascomycota phyla) fungi and those belonging to Sebacinales, Cantharellales, Agaricales, Polyporales, Filobasidiales and Tremellales orders (Basidiomycota phyla) were well represented in treated soil microcosms compared to control. Nevertheless, while for the treated SJF a complete recovery of the fungal community was observed at the end of the experiment, this was not the case for the P treated soil, although no more Bioherbicide remained. Indeed, the relative abundance of most of the saprophytic fungi were lower in treated soil compared to control microcosms whereas fungi from parasitic fungi included in Spizellomycetales and Pezizales orders increased. To the best of our knowledge, this is the only study assessing the effect of the Bioherbicide leptospermone on the composition and diversity of the fungal community in soil. This study showed that leptospermone has an impact on α- and β-diversity of the fungal community. It underlines the possible interest of microbial endpoints for environmental risk assessment of biopesticide.
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Table_1_Impact of Leptospermone, a Natural β-Triketone Herbicide, on the Fungal Composition and Diversity of Two Arable Soils.XLSX
2019Co-Authors: Clarisse Mallet, Sana Romdhane, Christophe Calvayrac, Fabrice Martin-laurent, Camille Loiseau, Jérémie Béguet, Lise BarthelmebsAbstract:Impact of leptospermone, a β-triketone Bioherbicide, was investigated on the fungal community which supports important soil ecological functions such as decomposition of organic matter and nutrients recycling. This study was done in a microcosm experiment using two French soils, Perpignan (P) and Saint-Jean-de-Fos (SJF), differing in their physicochemical properties and history treatment with synthetic β-triketones. Soil microcosms were treated with leptospermone at recommended dose and incubated under controlled conditions for 45 days. Untreated microcosms were used as control. Illumina MiSeq sequencing of the internal transcribed spacer region of the fungal rRNA revealed significant changes in fungal community structure and diversity in both soils. Xylariales, Hypocreales, Pleosporales and Capnodiales (Ascomycota phyla) fungi and those belonging to Sebacinales, Cantharellales, Agaricales, Polyporales, Filobasidiales and Tremellales orders (Basidiomycota phyla) were well represented in treated soil microcosms compared to control. Nevertheless, while for the treated SJF a complete recovery of the fungal community was observed at the end of the experiment, this was not the case for the P treated soil, although no more Bioherbicide remained. Indeed, the relative abundance of most of the saprophytic fungi were lower in treated soil compared to control microcosms whereas fungi from parasitic fungi included in Spizellomycetales and Pezizales orders increased. To the best of our knowledge, this is the only study assessing the effect of the Bioherbicide leptospermone on the composition and diversity of the fungal community in soil. This study showed that leptospermone has an impact on α- and β-diversity of the fungal community. It underlines the possible interest of microbial endpoints for environmental risk assessment of biopesticide.
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Devenir, biodégradation et impact écotoxicologique du Bioherbicide leptospermone dans les sols
2017Co-Authors: Sana Romdhane, Franck E. Dayan, Marion Devers-lamrani, Lise Barthelmebs, Christophe Calvayrac, Cédric Bertrand, Jean-françois Cooper, Fabrice Martin-laurentAbstract:Les herbicides sont appliqués dans le monde entier pour contrôler le développement et la propagation des plantes adventices afin d’assurer le rendement et la qualité des productions végétales. Toutefois, en raison de leur utilisation généralisée, des résidus d’herbicides persistent dans les sols agricoles d’où ils peuvent être transférés et contribués à la contamination de différents compartiments de l’environnement. L’utilisation de composés d’origine naturelle ayant une activité herbicide (Bioherbicide) est vue comme une alternative respectueuse de l’environnement pour la protection des cultures. Toutefois, malgré ce profil environnemental favorable, le devenir et l’impact écotoxicologique des Bioherbicides dans les sols ne sont pas connus. Dans ce contexte, nous avons étudié le devenir, la biodégradation et l’impact écotoxicologique du Bioherbicide leptospermone sur la communauté bactérienne des sols. La dissipation de la leptospermone a été caractérisée dans deux sols agricoles. Elle est sous le contrôle de processus abiotique (photolyse) et biotique (biodégradation). Methylophilus sp. LS1, une souche microbienne dégradante, a été isolée et caractérisée permettant d’identifier des produits de transformation, dont l’hydroxyleptospermone, et de proposer une voie de transformation de la leptospermone (Romdhane et al., 2017). L’impact écotoxicologique de la leptospermone sur la composition et la diversité α- et β- de la communauté bactérienne a été évalué. Nous avons montré que la communauté bactérienne des deux sols agricoles était sensible à la leptospermone. L’impact écotoxicologique était transitoire et la communauté bactérienne était résiliente dans le sol ou la dissipation de la leptospermone