The Experts below are selected from a list of 4821 Experts worldwide ranked by ideXlab platform
Guoqing Pan - One of the best experts on this subject based on the ideXlab platform.
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Multistimulus Responsive Biointerfaces with Switchable Bioadhesion and Surface Functions.
ACS Applied Materials & Interfaces, 2020Co-Authors: Yang Zhou, Yanjun Zheng, Ting Wei, Yaran Wang, Wenjun Zhan, Yanxia Zhang, Guoqing PanAbstract:Stimuli-responsive Biointerfaces can serve as dynamic tools for modulation of biointerfacial interactions. Considering the complexity of biological environments, surfaces with multistimulus respons...
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Biomimetic fabrication of dynamic Biointerfaces with optional and diversified bioactivities through reversible covalent and bioorthogonal chemistry
Chemical Engineering Journal, 2020Co-Authors: Xiaohua Tian, Xu Chen, Yonghai Feng, Yuqing Duan, Mingdong Dong, Guoqing Pan, Lei LiuAbstract:Abstract As extracellular matrix (ECM) mimics, dynamic Biointerfaces with reversible ligand presentation have shown great significance in the field of biology and medicine. However, current systems are trapped in the monotony of bioactivity, which makes it hard to mimic the multipotential of natural ECM. In this work, we reported a dynamic biomaterial interface with optional and diversified bioactivities by the combination of reversible catechol-boronate and bioorthogonal click chemistry. Due to the specificity and thoroughness of bioorthogonal reaction, different types of biomolecules including small molecular saccharide, macromolecular peptides and DNA aptamers could be on-demand and reversible binding on biomaterial interfaces through sugar-sensitive catechol-boronate interactions. In this design, the obtained dynamic Biointerface showed biocompatible sugar-responsiveness and enabled reversible presentation of diversified bioactivities, exhibiting the multipotential to manipulate a variety of cell-biomaterial interactions. Cell capture/release experiments confirmed our dynamic Biointerface could reversibly and selectively bind different cancer cells and even the bacterial microorganism. In short, apart from the original significance in ECM mimicking, the optional and diversified bioactivities on our developed dynamic Biointerface will also show promising prospects in biomedical science, in particular, the cell isolation area for diagnostics and therapeutics.
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Dynamic Synthetic Biointerfaces: From Reversible Chemical Interactions to Tunable Biological Effects
Accounts of Chemical Research, 2019Co-Authors: Tian Xiaohua, Lei Liu, Jianming Pan, Guoqing PanAbstract:ConspectusDynamic synthetic Biointerface is a new concept of biomaterials with smart surface properties capable of controlled display of bioactive ligands, dynamic modulation of cell-biomaterial interactions, and subsequently clever manipulation of fundamental cell behaviors like adhesion, migration, proliferation, differentiation, apoptosis, and so on. As mimics of the extracellular matrix (ECM), such molecularly dynamic Biointerfaces have attracted increasing attention because of their tunable biological effects with great significance in in situ cell biology, tissue engineering, drug targeting, and cell isolation for cancer theranostics.Approaches to control bioligand presentation on materials mainly rely on surface functionalization with dynamic or reversible chemical linkers to which the ligands are tethered. Photoelectric-transformable or photocleavable chemistry, host–guest supramolecular chemistry, and multiple noncovalent interactions were initially employed for fabrication of dynamic synthetic b...
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A Versatile Dynamic Mussel-Inspired Biointerface: From Specific Cell Behavior Modulation to Selective Cell Isolation.
Angewandte Chemie International Edition, 2018Co-Authors: Lei Liu, Xiaohua Tian, Yuqing Duan, Xin Zhao, Guoqing PanAbstract:Reported here is a novel dynamic Biointerface based on reversible catechol-boronate chemistry. Biomimetically designed peptides with a catechol-containing sequence and a cell-binding sequence at each end were initially obtained. The mussel-inspired peptides were then reversibly bound to a phenylboronic acid (PBA) containing polymer-grafted substrate through sugar-responsive catechol-boronate interactions. The resultant Biointerface is thus capable of dynamic presentation of the bioactivity (i.e. the cell-binding sequence) by virtue of changing sugar concentrations in the system (similar to human glycemic volatility). In addition, the sugar-responsive Biointerface enables not only dynamic modulation of stem cell adhesion behaviors but also selective isolation of tumor cells. Considering the highly biomimetic nature and biological stimuli-responsiveness, this mussel-inspired dynamic Biointerface holds great promise in both fundamental cell biology research and advanced medical applications.
Kazuhiko Ishihara - One of the best experts on this subject based on the ideXlab platform.
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cell membrane inspired phospholipid polymers for developing medical devices with excellent Biointerfaces
Science and Technology of Advanced Materials, 2012Co-Authors: Yasuhiko Iwasaki, Kazuhiko IshiharaAbstract:This review article describes fundamental aspects of cell membrane-inspired phospholipid polymers and their usefulness in the development of medical devices. Since the early 1990s, polymers composed of 2-methacryloyloxyethyl phosphorylcholine (MPC) units have been considered in the preparation of biomaterials. MPC polymers can provide an artificial cell membrane structure at the surface and serve as excellent Biointerfaces between artificial and biological systems. They have also been applied in the surface modification of some medical devices including long-term implantable artificial organs. An MPC polymer Biointerface can suppress unfavorable biological reactions such as protein adsorption and cell adhesion – in other words, specific biomolecules immobilized on an MPC polymer surface retain their original functions. MPC polymers are also being increasingly used for creating Biointerfaces with artificial cell membrane structures.
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A bioconjugated phospholipid polymer Biointerface with nanometer-scaled structure for highly sensitive immunoassays.
Methods In Molecular Biology Clifton Nj, 2011Co-Authors: Kazuki Nishizawa, Masaki Takai, Kazuhiko IshiharaAbstract:This method relates to the preparation of a phospholipid polymer platform and the immobilization of an antibody as a bioaffinity ligand onto the platform to construct a Biointerface for highly sensitive immunoassays. The specific phospholipid polymer used in this work is poly2-methacryloyloxyethyl phosphorylcholine (MPC)-co-n-butyl methacrylate (BMA)-co-p-nitrophenyloxycarbonyl poly(ethylene glycol) methacrylate (MEONP) (PMBN). The PMBN surface could immobilize specific antibodies through covalent chemical bonding by the reaction between MEONP units and amino groups in the antibody. In addition, the PMBN surface could prevent nonspecific protein adsorption from an analyte sample without the use of blocking reagents based on the fundamental properties of the MPC units. Furthermore, a nanometer-scaled particle deposition surface is constructed with PMBN by an electrospray deposition method to enhance the sensitivity by increasing the overall surface area of the Biointerface.
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Phospholipid Polymer Biointerfaces for Lab-on-a-Chip Devices
Annals of Biomedical Engineering, 2010Co-Authors: Yan Xu, Madoka Takai, Kazuhiko IshiharaAbstract:This review summarizes recent achievements and progress in the development of various functional 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer Biointerfaces for lab-on-a-chip devices and applications. As phospholipid polymers, MPC polymers can form cell-membrane-like surfaces by surface chemistry and physics and thereby provide Biointerfaces capable of suppressing protein adsorption and many subsequent biological responses. In order to enable application to microfluidic devices, a number of MPC polymers with diverse functions have been specially designed and synthesized by incorporating functional units such as charge and active ester for generating the microfluidic flow and conjugating biomolecules, respectively. Furthermore, these polymers were incorporated with silane or hydrophobic moiety to construct stable interfaces on various substrate materials such as glass, quartz, poly(methyl methacrylate), and poly(dimethylsiloxane), via a silane-coupling reaction or hydrophobic interactions. The basic interfacial properties of these interfaces have been characterized from multiple aspects of chemistry, physics, and biology, and the suppression of nonspecific bioadsorption and control of microfluidic flow have been successfully achieved using these Biointerfaces on a chip. Further, many chip-based biomedical applications such as immunoassays and DNA separation have been accomplished by integrating these Biointerfaces on a chip. Therefore, functional phospholipid polymer interfaces are promising and useful for application to lab-on-a-chip devices in biomedicine.
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Novel polymer biomaterials and interfaces inspired from cell membrane functions.
Biochimica et Biophysica Acta, 2010Co-Authors: Kazuhiko Ishihara, Yuuki Inoue, Yusuke Goto, Madoka Takai, Ryosuke Matsuno, Tomohiro KonnoAbstract:Abstract Background Materials with excellent biocompatibility on interfaces between artificial system and biological system are needed to develop any equipments and devices in bioscience, bioengineering and medicinal science. Suppression of unfavorable biological response on the interface is most important for understanding real functions of biomolecules on the surface. So, we should design and prepare such biomaterials. Scoop of review One of the best ways to design the biomaterials is generated from mimicking a cell membrane structure. It is composed of a phospholipid bilayered membrane and embedded proteins and polysaccharides. The surface of the cell membrane-like structure is constructed artificially by molecular integration of phospholipid polymer as platform and conjugated biomolecules. Here, it is introduced as the effectiveness of Biointerface with highly biological functions observed on artificial cell membrane structure. Major conclusions Reduction of nonspecific protein adsorption is essential for suppression of unfavorable bioresponse and achievement of versatile biomedical applications. Simultaneously, bioconjugation of biomolecules on the phospholipid polymer platform is crucial for a high-performance interface. General significance The Biointerfaces with both biocompatibility and biofunctionality based on biomolecules must be installed on advanced devices, which are applied in the fields of nanobioscience and nanomedicine. This article is part of a Special Issue entitled Nanotechnologies - Emerging Applications in Biomedicine.
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Establishing ultimate Biointerfaces covered with phosphorylcholine groups.
Colloids and Surfaces B: Biointerfaces, 2008Co-Authors: Junji Watanabe, Kazuhiko IshiharaAbstract:The phospholipid molecule is a typical component of the cell membrane. In particular, the phosphorylcholine polar group is an electrically neutral head group. 2-Methacryloyloxyethyl phosphorylcholine (MPC) comprising a phosphorylcholine group side chain was designed with the cell membrane as an inspiration. Versatile polymers comprising MPC could be synthesized, and their specific biofunctions were evaluated. Establishing an ultimate Biointerface with multiple functions is important from the viewpoint of biomaterials science. Nonspecific protein adsorption is essential for achieving versatile biomedical applications. Simultaneously, bioconjugation and retention of its biofunction are crucial for a high-performance Biointerface. In this review article, a tunable Biointerface comprising MPCs was introduced. In particular, the use of nanoparticles and polymer brushes as Biointerfaces was described along with the perspective versatility of their biological applications.
Celine Picard - One of the best experts on this subject based on the ideXlab platform.
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Skin surface physico-chemistry: Characteristics, methods of measurement, influencing factors and future developments
Advances in Colloid and Interface Science, 2019Co-Authors: Florine Eudier, Géraldine Savary, Michel Grisel, Celine PicardAbstract:Physico-chemical properties such as surface free energy, polarity or hydrophobicity of solid surfaces have been largely studied in literature because they are involved in many physical phenomena: adhesion, friction, wetting … Nowadays, the study of Biointerfaces is of great interest for the medical, the pharmaceutical or the cosmetic field but also for material design researches, especially for the development of biomimetic surfaces. The present paper focuses on a particular Biointerface, namely skin, which is the most extended organ of the human body. The different ways for the study of skin physico-chemistry are first reviewed, followed by their practical uses, from pharmaceutical to cosmetic science. Those properties depict the ways skin interacts with topical products, its lipid composition but also its hydration state. In addition, this article aims to present recent approaches using original model materials in order to mimic human skin; indeed, in vivo experiments are often limited by the inter and intra individual variability, the safety regulation and above all the time and the cost of such studies. Finally, further data clearly highlight the importance of skin surface properties for dermatological and pharmaceutical researches.
Lei Liu - One of the best experts on this subject based on the ideXlab platform.
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Biomimetic fabrication of dynamic Biointerfaces with optional and diversified bioactivities through reversible covalent and bioorthogonal chemistry
Chemical Engineering Journal, 2020Co-Authors: Xiaohua Tian, Xu Chen, Yonghai Feng, Yuqing Duan, Mingdong Dong, Guoqing Pan, Lei LiuAbstract:Abstract As extracellular matrix (ECM) mimics, dynamic Biointerfaces with reversible ligand presentation have shown great significance in the field of biology and medicine. However, current systems are trapped in the monotony of bioactivity, which makes it hard to mimic the multipotential of natural ECM. In this work, we reported a dynamic biomaterial interface with optional and diversified bioactivities by the combination of reversible catechol-boronate and bioorthogonal click chemistry. Due to the specificity and thoroughness of bioorthogonal reaction, different types of biomolecules including small molecular saccharide, macromolecular peptides and DNA aptamers could be on-demand and reversible binding on biomaterial interfaces through sugar-sensitive catechol-boronate interactions. In this design, the obtained dynamic Biointerface showed biocompatible sugar-responsiveness and enabled reversible presentation of diversified bioactivities, exhibiting the multipotential to manipulate a variety of cell-biomaterial interactions. Cell capture/release experiments confirmed our dynamic Biointerface could reversibly and selectively bind different cancer cells and even the bacterial microorganism. In short, apart from the original significance in ECM mimicking, the optional and diversified bioactivities on our developed dynamic Biointerface will also show promising prospects in biomedical science, in particular, the cell isolation area for diagnostics and therapeutics.
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Dynamic Synthetic Biointerfaces: From Reversible Chemical Interactions to Tunable Biological Effects
Accounts of Chemical Research, 2019Co-Authors: Tian Xiaohua, Lei Liu, Jianming Pan, Guoqing PanAbstract:ConspectusDynamic synthetic Biointerface is a new concept of biomaterials with smart surface properties capable of controlled display of bioactive ligands, dynamic modulation of cell-biomaterial interactions, and subsequently clever manipulation of fundamental cell behaviors like adhesion, migration, proliferation, differentiation, apoptosis, and so on. As mimics of the extracellular matrix (ECM), such molecularly dynamic Biointerfaces have attracted increasing attention because of their tunable biological effects with great significance in in situ cell biology, tissue engineering, drug targeting, and cell isolation for cancer theranostics.Approaches to control bioligand presentation on materials mainly rely on surface functionalization with dynamic or reversible chemical linkers to which the ligands are tethered. Photoelectric-transformable or photocleavable chemistry, host–guest supramolecular chemistry, and multiple noncovalent interactions were initially employed for fabrication of dynamic synthetic b...
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A Versatile Dynamic Mussel-Inspired Biointerface: From Specific Cell Behavior Modulation to Selective Cell Isolation.
Angewandte Chemie International Edition, 2018Co-Authors: Lei Liu, Xiaohua Tian, Yuqing Duan, Xin Zhao, Guoqing PanAbstract:Reported here is a novel dynamic Biointerface based on reversible catechol-boronate chemistry. Biomimetically designed peptides with a catechol-containing sequence and a cell-binding sequence at each end were initially obtained. The mussel-inspired peptides were then reversibly bound to a phenylboronic acid (PBA) containing polymer-grafted substrate through sugar-responsive catechol-boronate interactions. The resultant Biointerface is thus capable of dynamic presentation of the bioactivity (i.e. the cell-binding sequence) by virtue of changing sugar concentrations in the system (similar to human glycemic volatility). In addition, the sugar-responsive Biointerface enables not only dynamic modulation of stem cell adhesion behaviors but also selective isolation of tumor cells. Considering the highly biomimetic nature and biological stimuli-responsiveness, this mussel-inspired dynamic Biointerface holds great promise in both fundamental cell biology research and advanced medical applications.
Shawn D. Carrigan - One of the best experts on this subject based on the ideXlab platform.
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Development of hydrogel platforms for increased QCM-D Biointerface sensitivity in real-time immunoassay of sepsis-related biomarkers
2005Co-Authors: Shawn D. CarriganAbstract:This doctoral thesis describes the development of novel rapid deposition hydrogel platforms that serve as Biointerfaces for real-time immunoassay using quartz crystal microgravimetry (QCM). Biointerface development was undertaken with the goal of developing a simple system relying on affordable technology to achieve real-time immunoassay performance equivalent to more complex and involved protocols.%%%%The primary advantage of the hydrogel Biointerfaces developed herein lies in their rapid preparation using affordable, non-toxic reagents. Compositions developed over three sequential development cycles rely on chemically cross-linking carboxymethylcellulose, which serves to covalently immobilise recognition elements through amine coupling, to polyethyleneimine. The various compositions require 10 minutes or less to deposit, a substantial improvement over competing self-assembled monolayer protocols requiring incubations ranging from hours to days using highly toxic reagents. Additional benefit lies in the immunoassay functionality of the Biointerface, as these compositions excel in the traditional performance criteria of surface regeneration, minimisation of non-specific protein binding, and assay detection limit.%%%%The peak detection limit achieved using a sandwich assay for a 17 kDa cytokine was 25 ng/mL in buffer and 500 ng/mL in a 1:3 serum dilution, with generic immunoassay capability for other cytokines demonstrated. Reusability of the developed Biointerfaces is equally strong, with up to twenty regeneration cycles demonstrated without diminished sensitivity. Finally, mass-based estimates of non-specific serum adsorption indicate that the composition developed during the final design iteration equals the performance of the best protein-resistant Biointerfaces currently available in the literature.
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Rapid three-dimensional Biointerfaces for real-time immunoassay using hIL-18BPa as a model antigen.
Biomaterials, 2005Co-Authors: Shawn D. Carrigan, George Scott, Maryam TabrizianAbstract:Abstract With the goal of designing a rapid and affordable system of real-time immune monitoring for future diagnostic applications in sepsis, we have developed a Biointerface composed of polyethyleneimine (PEI) and carboxymethylcellulose (CMC) to provide a means of prompt and facile immunoassay. Biointerface assembly is complete within 30 min, with all preparation performed and monitored within the measurement chamber of a quartz crystal microgravimetry with dissipation (QCM-D) sensor. Optimised Biointerface composition, as determined by the mass of antibody immobilised, the level of antigen detection, and the amount of non-specific binding of human serum albumin, was determined to consist of a 4.0 mg/m L CMC hydrogel layer cross-linked to a 0.5 mg/m L PEI sub-layer. Tapping mode atomic force microscopy (AFM) in liquid demonstrates highly uniform and smooth surfaces using these hydrogels. Sensitivity of the Biointerface for rhIL-18BPa is 400 ng/m L , with detection of 1 μg/m L achievable following 25 surface regenerations. Performance of the Biointerface is verified using surface plasmon resonance (SPR), demonstrating the ability of the Biointerface to be applied across platforms.