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Kenichi Matsunaga - One of the best experts on this subject based on the ideXlab platform.

  • inhibition of decrease in natural killer cell activity in repeatedly restraint stressed mice by a Biological Response Modifier derived from cultured mycelia of the basidiomycete tricholoma matsutake
    Neuroimmunomodulation, 2004
    Co-Authors: Yoko Ishihara, Hiroko Iijima, Yoko Yagi, Hirotaka Hoshi, Kenichi Matsunaga
    Abstract:

    Objective: To develop a method to cope with stress-induced reduction in immunocompetence, we evaluated the immunomodulatory activities of a Biological Response Modifier derived from the mycelia of the basidiomycete Tricholoma matsutake (CM6271) in mice under repeated restraint stress. Methods: C57BL/6 mice were inserted, one per tube, into 50-ml polypropylene tubes into which more than 30 ventilation holes had been drilled, and were restrained everyday for 20 days in this fashion for set periods of time. Natural killer (NK) cell activity and NK1.1-positive cell counts in the spleen, ACTH and corticosterone levels in the blood were determined. CM6271 was orally administered daily during the restraint stress period. Results: (1) When the mice were restrained in a confined space for 6 h per day for 20 days, the NK cell activity and the NK1.1-positive cell counts in the spleen significantly decreased after day 5 with an increase in the blood ACTH and corticosterone levels. (2) Oral administration of CM6271 during the restraint stress period significantly prevented the stress-induced decrease in NK cell activity. The effect was dependent on the timing, duration, and doses administered. (3) CM6271 did not significantly affect the splenic NK1.1-positive cell counts or the levels of blood ACTH and corticosterone in restraint-stressed mice. Conclusion: The above findings suggest that CM6271 inhibits the restraint stress-induced decrease of NK cell activity in a timing of administration and dose-dependent manner.

  • enhanced recovery of nk cell activity in mice under restraint stress by the administration of a Biological Response Modifier derived from the mycelia of the basidiomycete tricholoma matsutake
    Stress, 2003
    Co-Authors: Yoko Ishihara, Hiroko Iijima, Yoko Yagi, Kenichi Matsunaga
    Abstract:

    Some types of stressor act on the immune system via the network comprising the endocrine-immune-nervous systems, and are reportedly responsible for the onset of diseases as well as giving impetus to their advance. It is important for the maintenance and promotion of health to cope with stress-induced changes in immunocompetence. Therefore, we studied the effects of administration of a novel Biological Response Modifier (CM6271) derived from the mycelia of the basidiomycete Tricholoma matsutake on the NK cell activity in mice under restraint stress, in order to evaluate its potential to modulate immune responsiveness in stress-loaded individuals. (1) When C57BL/6 mice were restrained in 50-ml tubes for more than 6 h, splenic NK cell activity decreased significantly, but recovered gradually after the mice were released. The extent of the reduction of activity and the degree of recovery depended on the duration of the restraint. (2) The oral administration of CM6271 caused a significant acceleration of the r...

  • effect of a Biological Response Modifier psk on intestinal flora of tumor bearing mice
    in Vivo, 1993
    Co-Authors: K Sakurai, Kenichi Matsunaga, T Fujii, Chikao Yoshikumi, I Motokawa, Takao Ando, Y Ohmura, K Saito, Kikuo Nomoto
    Abstract:

    PSK, a protein-bound polysaccharide derived from basidiomycetes, is a Biological Response Modifier that exhibits a variety of activities following oral administration, including prevention of infection. In this study, the effects of oral administration of this drug on microbial flora of tumor-bearing mice were examined. Numbers of Staphylococcus, Streptococcus and Pseudomonas were increased, and those of Bifidobacterium and Lactobacillus were reduced in fresh feces of mice from later than 9 weeks after inoculation of sarcoma 180. However, these changes were prevented by oral administration of PSK. In mice bearing sarcoma 180, the increases in numbers of Staphylococcus and Pseudomonas and the decrease in those of Bifidobacterium were further enhanced by intraperitoneal administration of anticancer agent mitomycin C, but such changes were suppressed by oral administration of PSK. These results suggest that PSK has a preventive effect against abnormal conditions of the intestinal flora induced by tumor inoculation or the administration of anticancer agents.

  • enhancement of effector cell activities in mice bearing syngeneic plasmacytoma x5563 by a Biological Response Modifier psk
    Journal of clinical & laboratory immunology, 1992
    Co-Authors: Kenichi Matsunaga, Hiroko Iijima, M Aota, Yoshiharu Oguchi, T Fujii, Chikao Yoshikumi, Kikuo Nomoto
    Abstract:

    : We investigated the effect of PSK, a protein-bound polysaccharide obtained from Coriolus versicolor of basidiomycetes, on antitumor immunity in tumor-bearing mice. PSK prolonged significantly the life span of C3H/He mice bearing syngeneic plasmacytoma X5563 in a schedule- and dose-dependent manner. PSK was most effective when administered at 100 mg/kg every other day ten times starting from the day after tumor inoculation. The administration of PSK enhanced significantly the cytostatic activity of peritoneal exudate plastic-adherent cells and the cytolytic activity of spleen cells after in vitro incubation with mitomycin C-treated tumor cells. In addition, PSK restored the cytokine-producing capacity of spleen cells suppressed in tumor-bearing mice after in vitro incubation with mitogen. Sera from tumor-bearing mice suppressed the activity of such effector cells as well as the interleukin 2-producing capacity of spleen cells, but sera from PSK-treated tumor-bearing mice prevented this suppression. These results suggest that PSK enhances antitumor immunity by reducing immunosuppressive activity of serum from tumor-bearing mice.

C Liu - One of the best experts on this subject based on the ideXlab platform.

  • macrophage activation by polysaccharide Biological Response Modifier isolated from aloe vera l var chinensis haw berg
    International Immunopharmacology, 2006
    Co-Authors: C Liu, M Y K Leung, J C M Koon, Lu Zhu, Y Z Hui, Kwokpui Fung
    Abstract:

    A mannose-rich polysaccharide Biological Response Modifier (BRM), derived from Aloe vera L. var. chinensis (Haw.) Berg., was demonstrated to be a potent murine B- and T-cell stimulator in our previous study. We here report the stimulatory activity of PAC-I on murine peritoneal macrophage. The polysaccharide when injected into mice enhanced the migration of macrophages to the peritoneal cavity. Peritoneal macrophage when treated by PAC-I in vitro had increased expression of MHC-II and FcγR, and enhanced endocytosis, phagocytosis, nitric oxide production, TNF-α secretion and tumor cell cytotoxicity. The administration of PAC-I into allogeneic ICR mice stimulated systemic TNF-α production in a dose-dependent manner and prolonged the survival of tumor-bearing mice. PAC-I is thus a potent stimulator of murine macrophage and the in vitro observed tumoricidal properties of activated macrophage might account for the in vivo antitumor properties of PAC-I. Our research findings may have therapeutic implications in tumor immunotherapy.

  • chemical and Biological characterization of a polysaccharide Biological Response Modifier from aloe vera l var chinensis haw berg
    Glycobiology, 2004
    Co-Authors: M Y K Leung, C Liu, Y Z Hui, L F Zhu, K P Fung
    Abstract:

    Three purified polysaccharide fractions designated as PAC-I, PAC-II, and PAC-III were prepared from Aloe vera L. var. chinensis (Haw.) Berg. by membrane fractionation and gel filtration HPLC. The polysaccharide fractions had molecular weights of 10,000 kDa, 1300 kDa, and 470 kDa, respectively. The major sugar residue in the polysaccharide fractions is mannose, which was found to be91.5% in PAC-I, 87.9% in PAC-II, and 53.7% in PAC-III. The protein contents in the polysaccharide fractions was undetectable. NMR study of PAC-I and PAC-II demonstrated the polysaccharides shared the same structure. The main skeletons of PAC-I and PAC-II are beta-(1-->4)-D linked mannose with acetylation at C-6 of manopyranosyl. The polysaccharide fractions stimulated peritoneal macrophages, splenic T and B cell proliferation, and activated these cells to secrete TNF-alpha, IL-1beta, INF-gamma, IL-2, and IL-6. The polysaccharides were nontoxic and exhibited potent indirect antitumor Response in murine model. PAC-I, which had the highest mannose content and molecular weight, was found to be the most potent Biological Response Modifier of the three fractions. Our results suggested that the potency of aloe polysaccharide fraction increases as mannose content and molecular weight of the polysaccharide fraction increase.

M Y K Leung - One of the best experts on this subject based on the ideXlab platform.

  • macrophage activation by polysaccharide Biological Response Modifier isolated from aloe vera l var chinensis haw berg
    International Immunopharmacology, 2006
    Co-Authors: C Liu, M Y K Leung, J C M Koon, Lu Zhu, Y Z Hui, Kwokpui Fung
    Abstract:

    A mannose-rich polysaccharide Biological Response Modifier (BRM), derived from Aloe vera L. var. chinensis (Haw.) Berg., was demonstrated to be a potent murine B- and T-cell stimulator in our previous study. We here report the stimulatory activity of PAC-I on murine peritoneal macrophage. The polysaccharide when injected into mice enhanced the migration of macrophages to the peritoneal cavity. Peritoneal macrophage when treated by PAC-I in vitro had increased expression of MHC-II and FcγR, and enhanced endocytosis, phagocytosis, nitric oxide production, TNF-α secretion and tumor cell cytotoxicity. The administration of PAC-I into allogeneic ICR mice stimulated systemic TNF-α production in a dose-dependent manner and prolonged the survival of tumor-bearing mice. PAC-I is thus a potent stimulator of murine macrophage and the in vitro observed tumoricidal properties of activated macrophage might account for the in vivo antitumor properties of PAC-I. Our research findings may have therapeutic implications in tumor immunotherapy.

  • chemical and Biological characterization of a polysaccharide Biological Response Modifier from aloe vera l var chinensis haw berg
    Glycobiology, 2004
    Co-Authors: M Y K Leung, C Liu, Y Z Hui, L F Zhu, K P Fung
    Abstract:

    Three purified polysaccharide fractions designated as PAC-I, PAC-II, and PAC-III were prepared from Aloe vera L. var. chinensis (Haw.) Berg. by membrane fractionation and gel filtration HPLC. The polysaccharide fractions had molecular weights of 10,000 kDa, 1300 kDa, and 470 kDa, respectively. The major sugar residue in the polysaccharide fractions is mannose, which was found to be91.5% in PAC-I, 87.9% in PAC-II, and 53.7% in PAC-III. The protein contents in the polysaccharide fractions was undetectable. NMR study of PAC-I and PAC-II demonstrated the polysaccharides shared the same structure. The main skeletons of PAC-I and PAC-II are beta-(1-->4)-D linked mannose with acetylation at C-6 of manopyranosyl. The polysaccharide fractions stimulated peritoneal macrophages, splenic T and B cell proliferation, and activated these cells to secrete TNF-alpha, IL-1beta, INF-gamma, IL-2, and IL-6. The polysaccharides were nontoxic and exhibited potent indirect antitumor Response in murine model. PAC-I, which had the highest mannose content and molecular weight, was found to be the most potent Biological Response Modifier of the three fractions. Our results suggested that the potency of aloe polysaccharide fraction increases as mannose content and molecular weight of the polysaccharide fraction increase.

Kwokpui Fung - One of the best experts on this subject based on the ideXlab platform.

  • macrophage activation by polysaccharide Biological Response Modifier isolated from aloe vera l var chinensis haw berg
    International Immunopharmacology, 2006
    Co-Authors: C Liu, M Y K Leung, J C M Koon, Lu Zhu, Y Z Hui, Kwokpui Fung
    Abstract:

    A mannose-rich polysaccharide Biological Response Modifier (BRM), derived from Aloe vera L. var. chinensis (Haw.) Berg., was demonstrated to be a potent murine B- and T-cell stimulator in our previous study. We here report the stimulatory activity of PAC-I on murine peritoneal macrophage. The polysaccharide when injected into mice enhanced the migration of macrophages to the peritoneal cavity. Peritoneal macrophage when treated by PAC-I in vitro had increased expression of MHC-II and FcγR, and enhanced endocytosis, phagocytosis, nitric oxide production, TNF-α secretion and tumor cell cytotoxicity. The administration of PAC-I into allogeneic ICR mice stimulated systemic TNF-α production in a dose-dependent manner and prolonged the survival of tumor-bearing mice. PAC-I is thus a potent stimulator of murine macrophage and the in vitro observed tumoricidal properties of activated macrophage might account for the in vivo antitumor properties of PAC-I. Our research findings may have therapeutic implications in tumor immunotherapy.

Y Z Hui - One of the best experts on this subject based on the ideXlab platform.

  • macrophage activation by polysaccharide Biological Response Modifier isolated from aloe vera l var chinensis haw berg
    International Immunopharmacology, 2006
    Co-Authors: C Liu, M Y K Leung, J C M Koon, Lu Zhu, Y Z Hui, Kwokpui Fung
    Abstract:

    A mannose-rich polysaccharide Biological Response Modifier (BRM), derived from Aloe vera L. var. chinensis (Haw.) Berg., was demonstrated to be a potent murine B- and T-cell stimulator in our previous study. We here report the stimulatory activity of PAC-I on murine peritoneal macrophage. The polysaccharide when injected into mice enhanced the migration of macrophages to the peritoneal cavity. Peritoneal macrophage when treated by PAC-I in vitro had increased expression of MHC-II and FcγR, and enhanced endocytosis, phagocytosis, nitric oxide production, TNF-α secretion and tumor cell cytotoxicity. The administration of PAC-I into allogeneic ICR mice stimulated systemic TNF-α production in a dose-dependent manner and prolonged the survival of tumor-bearing mice. PAC-I is thus a potent stimulator of murine macrophage and the in vitro observed tumoricidal properties of activated macrophage might account for the in vivo antitumor properties of PAC-I. Our research findings may have therapeutic implications in tumor immunotherapy.

  • chemical and Biological characterization of a polysaccharide Biological Response Modifier from aloe vera l var chinensis haw berg
    Glycobiology, 2004
    Co-Authors: M Y K Leung, C Liu, Y Z Hui, L F Zhu, K P Fung
    Abstract:

    Three purified polysaccharide fractions designated as PAC-I, PAC-II, and PAC-III were prepared from Aloe vera L. var. chinensis (Haw.) Berg. by membrane fractionation and gel filtration HPLC. The polysaccharide fractions had molecular weights of 10,000 kDa, 1300 kDa, and 470 kDa, respectively. The major sugar residue in the polysaccharide fractions is mannose, which was found to be91.5% in PAC-I, 87.9% in PAC-II, and 53.7% in PAC-III. The protein contents in the polysaccharide fractions was undetectable. NMR study of PAC-I and PAC-II demonstrated the polysaccharides shared the same structure. The main skeletons of PAC-I and PAC-II are beta-(1-->4)-D linked mannose with acetylation at C-6 of manopyranosyl. The polysaccharide fractions stimulated peritoneal macrophages, splenic T and B cell proliferation, and activated these cells to secrete TNF-alpha, IL-1beta, INF-gamma, IL-2, and IL-6. The polysaccharides were nontoxic and exhibited potent indirect antitumor Response in murine model. PAC-I, which had the highest mannose content and molecular weight, was found to be the most potent Biological Response Modifier of the three fractions. Our results suggested that the potency of aloe polysaccharide fraction increases as mannose content and molecular weight of the polysaccharide fraction increase.