Tricholoma

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Akiyoshi Yamada - One of the best experts on this subject based on the ideXlab platform.

  • Mobile DNA distributions refine the phylogeny of “matsutake” mushrooms, Tricholoma sect. Caligata
    Mycorrhiza, 2013
    Co-Authors: Hitoshi Murata, Muneyoshi Yamaguchi, Akiyoshi Yamada, Shinichiro Katahata, Yuichiro Otsuka, Katsuhiko Babasaki, Hitoshi Neda
    Abstract:

    “Matsutake” mushrooms are formed by several species of Tricholoma sect. Caligata distributed across the northern hemisphere. A phylogenetic analysis of matsutake based on virtually neutral mutations in DNA sequences resolved robust relationships among Tricholoma anatolicum , Tricholoma bakamatsutake , Tricholoma magnivelare , Tricholoma matsutake , and Tricholoma sp. from Mexico (= Tricholoma sp. Mex). However, relationships among these matsutake and other species, such as Tricholoma caligatum and Tricholoma fulvocastaneum , were ambiguous. We, therefore, analyzed genomic copy numbers of σ _ marY1 , marY1 , and marY2N retrotransposons by comparing them with the single-copy mobile DNA megB1 using real-time polymerase chain reaction (PCR) to clarify matsutake phylogeny. We also examined types of megB1 -associated domains, composed of a number of poly (A) and poly (T) reminiscent of RNA-derived DNA elements among these species. Both datasets resolved two distinct groups, one composed of T. bakamatsutake , T. fulvocastaneum , and T. caligatum that could have diverged earlier and the other comprising T. magnivelare , Tricholoma sp. Mex, T. anatolicum , and T. matsutake that could have evolved later. In the first group, T. caligatum was the closest to the second group, followed by T. fulvocastaneum and T. bakamatsutake . Within the second group, T. magnivelare was clearly differentiated from the other species. The data suggest that matsutake underwent substantial evolution between the first group, mostly composed of Fagaceae symbionts, and the second group, comprised only of Pinaceae symbionts, but diverged little within each groups. Mobile DNA markers could be useful in resolving difficult phylogenies due to, for example, closely spaced speciation events.

  • in vitro ectomycorrhizal specificity between the asian red pine pinus densiflora and Tricholoma matsutake and allied species from worldwide pinaceae and fagaceae forests
    Mycorrhiza, 2010
    Co-Authors: Akiyoshi Yamada, Hitoshi Murata, Hisayasu Kobayashi, Erbil Kalmis, Fatih Kalyoncu, Masaki Fukuda
    Abstract:

    Tricholoma matsutake produces commercially valuable, yet uncultivable, mushrooms (matsutake) in association with pines in the Far East and Scandinavia and with both pines and oaks in the foothills of Tibet. Other matsutake mushrooms, such as Tricholoma anatolicum from the Mediterranean regions and Tricholoma magnivelare and Tricholoma sp. from the North Pacific Coast area of Canada and North America as well as Mexico, respectively, are associated with pines or oaks in their natural habitats. Tricholoma bakamatsutake and Tricholoma fulvocastaneum from Asia produce moderately valuable matsutake mushrooms and are solely associated with Fagaceae in nature. In this study, we demonstrate for the first time that matsutake mushrooms from Scandinavia, Mediterranean regions, North America, and Tibet form ectomycorrhizae with Pinus densiflora similar to the Far East T. matsutake. In general, worldwide T. matsutake and the symbionts of Pinaceae colonize the rhizospheres of P. densiflora as well as T. matsutake isolated from the host plant. However, T. fulvocastaneum and T. bakamatsutake formed a discontinuous Hartig net and no Hartig net, respectively, and colonized to a lesser extent as compared to T. matsutake. The data suggest that conifer-associated matsutake mushrooms in their native habitat will associate symbiotically with the Asian red pine.

  • development of microsatellite markers from an ectomycorrhizal fungus Tricholoma matsutake by an issr suppression pcr method
    Mycorrhiza, 2003
    Co-Authors: Chunlan Lian, Taizo Hogetsu, Norihisa Matsushita, Alexis Guerinlaguette, Kazuo Suzuki, Akiyoshi Yamada
    Abstract:

    An inter-simple sequence repeat (ISSR)-suppression-PCR technique established to develop microsatellite markers of plant species was applied to an ectomycorrhizal fungus, Tricholoma matsutake. Six polymorphic SSR markers were developed. All six polymorphic SSR markers were single-locused and co-dominant. Alleles produced by these six single-locused markers ranged from two to nine per locus and the expected heterozygosities were calculated as values from 0.098 to 0.803. The results indicated that the ISSR-suppression-PCR technique was effective and applicable to the development of microsatellite markers from ectomycorrhizal fungi. Furthermore, the six microsatellite loci did not amplify DNA from any other ectomycorrhizal species investigated, except for Tricholoma nauseosum (Swedish matsutake) and Tricholoma fulvocastaneum, suggesting that population genetics and reproduction of T. matsutake could be investigated by the SSR markers developed in the present study.

Hitoshi Murata - One of the best experts on this subject based on the ideXlab platform.

  • Agar plate assays using dye-linked substrates differentiate members of Tricholoma sect. Caligata, ectomycorrhizal symbionts represented by Tricholoma matsutake
    Mycoscience, 2017
    Co-Authors: Tomoko Shimokawa, Muneyoshi Yamaguchi, Hitoshi Murata
    Abstract:

    Abstract Tricholoma matsutake is an ectomycorrhizal fungus that produces the prized mushrooms “matsutake” in association with the Pinaceae. Other species of Tricholoma sect. Caligata are also ectomycorrhizal symbionts that produce a variety of “ quasi -matsutake” mushrooms. Here we developed agar plate assays using the dye-linked substrates azurine-crosslinked (AZCL) hydroxyethyl cellulose and AZCL-amylose to differentiate T. matsutake strains and related species based on their polysaccharide-degrading activities. This method may be useful for screening strains that adapt well to spawn cultivation for mushroom fruiting.

  • Mobile DNA distributions refine the phylogeny of “matsutake” mushrooms, Tricholoma sect. Caligata
    Mycorrhiza, 2013
    Co-Authors: Hitoshi Murata, Muneyoshi Yamaguchi, Akiyoshi Yamada, Shinichiro Katahata, Yuichiro Otsuka, Katsuhiko Babasaki, Hitoshi Neda
    Abstract:

    “Matsutake” mushrooms are formed by several species of Tricholoma sect. Caligata distributed across the northern hemisphere. A phylogenetic analysis of matsutake based on virtually neutral mutations in DNA sequences resolved robust relationships among Tricholoma anatolicum , Tricholoma bakamatsutake , Tricholoma magnivelare , Tricholoma matsutake , and Tricholoma sp. from Mexico (= Tricholoma sp. Mex). However, relationships among these matsutake and other species, such as Tricholoma caligatum and Tricholoma fulvocastaneum , were ambiguous. We, therefore, analyzed genomic copy numbers of σ _ marY1 , marY1 , and marY2N retrotransposons by comparing them with the single-copy mobile DNA megB1 using real-time polymerase chain reaction (PCR) to clarify matsutake phylogeny. We also examined types of megB1 -associated domains, composed of a number of poly (A) and poly (T) reminiscent of RNA-derived DNA elements among these species. Both datasets resolved two distinct groups, one composed of T. bakamatsutake , T. fulvocastaneum , and T. caligatum that could have diverged earlier and the other comprising T. magnivelare , Tricholoma sp. Mex, T. anatolicum , and T. matsutake that could have evolved later. In the first group, T. caligatum was the closest to the second group, followed by T. fulvocastaneum and T. bakamatsutake . Within the second group, T. magnivelare was clearly differentiated from the other species. The data suggest that matsutake underwent substantial evolution between the first group, mostly composed of Fagaceae symbionts, and the second group, comprised only of Pinaceae symbionts, but diverged little within each groups. Mobile DNA markers could be useful in resolving difficult phylogenies due to, for example, closely spaced speciation events.

  • in vitro ectomycorrhizal specificity between the asian red pine pinus densiflora and Tricholoma matsutake and allied species from worldwide pinaceae and fagaceae forests
    Mycorrhiza, 2010
    Co-Authors: Akiyoshi Yamada, Hitoshi Murata, Hisayasu Kobayashi, Erbil Kalmis, Fatih Kalyoncu, Masaki Fukuda
    Abstract:

    Tricholoma matsutake produces commercially valuable, yet uncultivable, mushrooms (matsutake) in association with pines in the Far East and Scandinavia and with both pines and oaks in the foothills of Tibet. Other matsutake mushrooms, such as Tricholoma anatolicum from the Mediterranean regions and Tricholoma magnivelare and Tricholoma sp. from the North Pacific Coast area of Canada and North America as well as Mexico, respectively, are associated with pines or oaks in their natural habitats. Tricholoma bakamatsutake and Tricholoma fulvocastaneum from Asia produce moderately valuable matsutake mushrooms and are solely associated with Fagaceae in nature. In this study, we demonstrate for the first time that matsutake mushrooms from Scandinavia, Mediterranean regions, North America, and Tibet form ectomycorrhizae with Pinus densiflora similar to the Far East T. matsutake. In general, worldwide T. matsutake and the symbionts of Pinaceae colonize the rhizospheres of P. densiflora as well as T. matsutake isolated from the host plant. However, T. fulvocastaneum and T. bakamatsutake formed a discontinuous Hartig net and no Hartig net, respectively, and colonized to a lesser extent as compared to T. matsutake. The data suggest that conifer-associated matsutake mushrooms in their native habitat will associate symbiotically with the Asian red pine.

Monique Gardes - One of the best experts on this subject based on the ideXlab platform.

  • phylogenetic species delimitation in ectomycorrhizal fungi and implications for barcoding the case of the Tricholoma scalpturatum complex basidiomycota
    Molecular Ecology, 2010
    Co-Authors: Patricia Jargeat, Florent Martos, Fabian Carriconde, Herve Gryta, Pierrearthur Moreau, Monique Gardes
    Abstract:

    Population studies have revealed that the fungal ectomycorrhizal morphospecies Tricholoma scalpturatum consists of at least two genetically distinct groups that occur sympatrically in several geographical areas. This discovery prompted us to examine species boundaries and relationships between members formerly assigned to T. scalpturatum and allied taxa using phylogenetic analyses. Sequence data were obtained from three nuclear DNA regions [internal transcribed spacer (ITS), gpd and tef], from 101 carpophores collected over a large geographical range in Western Europe, and some reference sequences from public databases. The ITS was also tested for its applicability as DNA barcode for species delimitation. Four highly supported phylogenetic clades were detected. The two previously detected genetic groups of T. scalpturatum were assigned to the phylospecies Tricholoma argyraceum and T. scalpturatum. The two remaining clades were referred to as Tricholoma cingulatum and Tricholoma inocybeoides. Unexpectedly, T. cingulatum showed an accelerated rate of evolution that we attributed to narrow host specialization. This study also reveals recombinant ITS sequences in T. inocybeoides, suggesting a hybrid origin. The ITS was a useful tool for the determination of species boundaries: the mean value of intraspecific genetic distances in the entire ITS region (including 5.8S rDNA) was <0.2%, whereas interspecific divergence estimates ranged from 1.78% to 4.22%. Apart from giving insights into the evolution of the T. scalpturatum complex, this study contributes to the establishment of a library of taxonomically verified voucher specimens, an a posteriori correlation between phenotype and genotype, and DNA barcoding of ectomycorrhizal fungi.

  • population dynamics of the ectomycorrhizal fungal species Tricholoma populinum and Tricholoma scalpturatum associated with black poplar under differing environmental conditions
    Environmental Microbiology, 2006
    Co-Authors: Herve Gryta, Patricia Jargeat, Fabian Carriconde, Jeanyves Charcosset, Monique Gardes
    Abstract:

    Summary Fungi combine sexual reproduction and clonal propagation. The balance between these two reproductive modes affects establishment dynamics, and ultimately the evolutionary potential of populations. The pattern of colonization was studied in two species of ectomycorrhizal fungi: Tricholoma populinum and Tricholoma scalpturatum . The former is considered to be a host specialist whereas T. scalpturatum is a generalist taxon. Fruit bodies of both basidiomycete species were mapped and collected over several years from a black poplar ( Populus nigra ) stand, at two different sites. Multilocus genotypes ( = genets) were identified based on the analysis of random amplified polymorphic DNA (RAPD) patterns, inter-simple sequence repeat (ISSR) patterns and restriction fragment length polymorphisms (RFLPs) in the ribosomal DNA intergenic spacer (rDNA IGS). The genetic analyses revealed differences in local population dynamics between the two species. Tricholoma scalpturatum tended to capture new space through sexual spores whereas T. populinum did this by clonal growth, suggesting trade-offs in allocation of resources at the genet level. Genet numbers and sizes strongly differ between the two study sites, perhaps as a result of abiotic disturbance on mycelial establishment and genet behaviour.

Hyungkyoon Choi - One of the best experts on this subject based on the ideXlab platform.

  • characterization of aroma active compounds in raw and cooked pine mushrooms Tricholoma matsutake sing
    Journal of Agricultural and Food Chemistry, 2006
    Co-Authors: Hyungkyoon Choi
    Abstract:

    The characteristic aroma-active compounds in raw and cooked pine-mushrooms (Tricholoma matsutake Sing.) were investigated by gas chromatography−olfactometry using aroma extract dilution analysis. 1-Octen-3-one (mushroom-like) was the major aroma-active compound in raw pine-mushrooms; this compound had the highest flavor dilution factor, followed by ethyl 2-methylbutyrate (floral and sweet), linalool (citrus-like), methional (boiled potato-like), 3-octanol (mushroom-like and buttery), 1-octen-3-ol (mushroom-like), (E)-2-octen-1-ol (mushroom-like), and 3-octanone (mushroom-like and buttery). By contrast, methional, 2-acetylthiazole (roasted), an unknown compound (chocolate-like), 3-hydroxy-2-butanone (buttery), and phenylacetaldehyde (floral and sweet), which could be formed by diverse thermal reactions during the cooking process, together with C8 compounds, were identified as the major aroma-active compounds in cooked pine-mushrooms. Keywords: Pine-mushroom (Tricholoma matsutake Sing.); aroma-active compou...

Patricia Jargeat - One of the best experts on this subject based on the ideXlab platform.

  • phylogenetic species delimitation in ectomycorrhizal fungi and implications for barcoding the case of the Tricholoma scalpturatum complex basidiomycota
    Molecular Ecology, 2010
    Co-Authors: Patricia Jargeat, Florent Martos, Fabian Carriconde, Herve Gryta, Pierrearthur Moreau, Monique Gardes
    Abstract:

    Population studies have revealed that the fungal ectomycorrhizal morphospecies Tricholoma scalpturatum consists of at least two genetically distinct groups that occur sympatrically in several geographical areas. This discovery prompted us to examine species boundaries and relationships between members formerly assigned to T. scalpturatum and allied taxa using phylogenetic analyses. Sequence data were obtained from three nuclear DNA regions [internal transcribed spacer (ITS), gpd and tef], from 101 carpophores collected over a large geographical range in Western Europe, and some reference sequences from public databases. The ITS was also tested for its applicability as DNA barcode for species delimitation. Four highly supported phylogenetic clades were detected. The two previously detected genetic groups of T. scalpturatum were assigned to the phylospecies Tricholoma argyraceum and T. scalpturatum. The two remaining clades were referred to as Tricholoma cingulatum and Tricholoma inocybeoides. Unexpectedly, T. cingulatum showed an accelerated rate of evolution that we attributed to narrow host specialization. This study also reveals recombinant ITS sequences in T. inocybeoides, suggesting a hybrid origin. The ITS was a useful tool for the determination of species boundaries: the mean value of intraspecific genetic distances in the entire ITS region (including 5.8S rDNA) was <0.2%, whereas interspecific divergence estimates ranged from 1.78% to 4.22%. Apart from giving insights into the evolution of the T. scalpturatum complex, this study contributes to the establishment of a library of taxonomically verified voucher specimens, an a posteriori correlation between phenotype and genotype, and DNA barcoding of ectomycorrhizal fungi.

  • population dynamics of the ectomycorrhizal fungal species Tricholoma populinum and Tricholoma scalpturatum associated with black poplar under differing environmental conditions
    Environmental Microbiology, 2006
    Co-Authors: Herve Gryta, Patricia Jargeat, Fabian Carriconde, Jeanyves Charcosset, Monique Gardes
    Abstract:

    Summary Fungi combine sexual reproduction and clonal propagation. The balance between these two reproductive modes affects establishment dynamics, and ultimately the evolutionary potential of populations. The pattern of colonization was studied in two species of ectomycorrhizal fungi: Tricholoma populinum and Tricholoma scalpturatum . The former is considered to be a host specialist whereas T. scalpturatum is a generalist taxon. Fruit bodies of both basidiomycete species were mapped and collected over several years from a black poplar ( Populus nigra ) stand, at two different sites. Multilocus genotypes ( = genets) were identified based on the analysis of random amplified polymorphic DNA (RAPD) patterns, inter-simple sequence repeat (ISSR) patterns and restriction fragment length polymorphisms (RFLPs) in the ribosomal DNA intergenic spacer (rDNA IGS). The genetic analyses revealed differences in local population dynamics between the two species. Tricholoma scalpturatum tended to capture new space through sexual spores whereas T. populinum did this by clonal growth, suggesting trade-offs in allocation of resources at the genet level. Genet numbers and sizes strongly differ between the two study sites, perhaps as a result of abiotic disturbance on mycelial establishment and genet behaviour.