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Omar Dos Santos Carvalho - One of the best experts on this subject based on the ideXlab platform.
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use of molecular methods for the rapid mass detection of schistosoma mansoni platyhelminthes trematoda in Biomphalaria spp gastropoda planorbidae
Journal of Tropical Medicine, 2017Co-Authors: Roberta Lima Caldeira, Liana K Jannottipassos, Omar Dos Santos CarvalhoAbstract:The low stringency-polymerase chain reaction (LS-PCR) and loop-mediated isothermal amplification (LAMP) assays were used to detect the presence of S. mansoni DNA in (1) Brazilian intermediate hosts (Biomphalaria glabrata, B. straminea, and B. tenagophila) with patent S. mansoni infections, (2) B. glabrata snails with prepatent S. mansoni infections, (3) various mixtures of infected and noninfected snails; and (4) snails infected with other trematode species. The assays showed high sensitivity and specificity and could detect S. mansoni DNA when one positive snail was included in a pool of 1,000 negative specimens of Biomphalaria. These molecular approaches can provide a low-cost, effective, and rapid method for detecting the presence of S. mansoni in pooled samples of field-collected Biomphalaria. These assays should aid mapping of transmission sites in endemic areas, especially in low prevalence regions and improve schistosomiasis surveillance. It will be a useful tool to monitor low infection rates of snails in areas where control interventions are leading towards the elimination of schistosomiasis.
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characterization of south american snails of the genus Biomphalaria basommatophora planorbidae and schistosoma mansoni platyhelminthes trematoda in molluscs by pcr rflp
BioMed Research International, 2016Co-Authors: Roberta Lima Caldeira, Liana K Jannottipassos, Tatiana Maria Teodoro, Pollanah M Liramoreira, Christiane De Oliveira Goveia, Omar Dos Santos CarvalhoAbstract:The identification of snails of the genus Biomphalaria can be done using morphological characteristics which depends on the size of the snails and skill and knowledge of researcher. These methods sometimes are not adequate for identification of species. The PCR-RFLP, using the ITS region of the rDNA, has been used to identify Brazilian species of the genus Biomphalaria. Nevertheless, there is a lack of information about snails from other Latin American countries. In addition, some snails may be infected by Schistosoma mansoni and when submitted to PCR-RFLP they show molecular profiles different from those previously standardized for the other mollusc species. In this work the molecular profiles of 15 species and the subspecies were established by PCR-RFLP of ITS-rDNA with the enzyme DdeI. Moreover, the molecular profiles of host species, B. glabrata, B. straminea, B. tenagophila, and B. prona, infected by S. mansoni were also established. The molluscs were dissected to permit morphological identification. These results contribute to a correct identification of snails of the genus Biomphalaria and detection of these snails infected by S. mansoni.
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Biomphalaria molluscs gastropoda planorbidae in rio grande do sul brazil
Memorias Do Instituto Oswaldo Cruz, 2009Co-Authors: Michele Soares Pepe, Roberta Lima Caldeira, Omar Dos Santos Carvalho, Gertrud Muller, Liana K Jannottipassos, Alice Pozza Rodrigues, Hugo Leonardo Da Cunha Amaral, Maria Elisabeth Aires BerneAbstract:The present study was aimed at characterising Biomphalaria species using both morphological and molecular (PCR-RFLP) approaches. The specimens were collected in 15 localities in 12 municipalities of the southern region of the state of Rio Grande do Sul, Brazil. The following species were found and identified: Biomphalaria tenagophila guaibensis, Biomphalaria oligoza and Biomphalaria peregrina. Specimens of the latter species were experimentally challenged with the LE Schistosoma mansoni strain, which showed to be refractory to infection.
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phylogenetics of south american Biomphalaria and description of a new species gastropoda planorbidae
Journal of Molluscan Studies, 2006Co-Authors: Roberta Lima Caldeira, Victoria Estrada, Luz Elena Velasquez, Eduar Elias Bejarano, Winston Rojas, Omar Dos Santos CarvalhoAbstract:The planorbid snail Biomphalaria edisoni n. sp. is described from morphological and molecular characters, based on specimens collected at two localities in Colombia. The new species has the typical features of the genus, as well as the following characteristics: vaginal pouch, numerous prostatic diverticula arranged in racemes, penis sheath/prepuce ratio of approximately 2 and a penial complex half the length of the cephalic portion of the female duct. These elements differentiate it from B. kuhniana, which was also present in one of the localities, and also from B. prona, B. helophila and B. nicaraguana, with which it has greater morphological similarities. Phylogenetic analysis of the nucleotide sequences of the internal spacers of the ribosomal DNA transcript (ITS1, ITS2) of B. edisoni n. sp. and 14 neotropical Biomphalaria species indicates that the former belongs to the monophyletic group including B. prona and B. sp. from Cuba. The genetic distances within this monophyletic group are however great enough for B. edisoni n. sp. to be regarded as a separate species. Thus morphology, phylogenetic tree topology and genetic distances all support the recognition of B. edisoni as a new species.
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multiplex pcr for both identification of brazilian Biomphalaria species gastropoda planorbidae and diagnosis of infection by schistosoma mansoni trematoda schistosomatidae
Journal of Parasitology, 2006Co-Authors: Liana K Jannottipassos, Omar Dos Santos Carvalho, Kelly Grace Magalhaes, Teofânia H D A VidigalAbstract:A simple and single-step technique based on multiplex PCR (multiplex polymerase chain reaction) has been developed for simultaneous identification of Brazilian Biomphalaria species, the intermediate hosts of Schistosoma mansoni, and their diagnosis of infection by the trematode. We used species-specific primers directed both to the internal transcribed spacer 2 (ITS2) of ribosomal DNA from 3 of the S. mansoni host species and to the mitochondrial DNA (mtDNA) from the trematode. Those primers were used simultaneously in a single multiplex-PCR reaction, and template DNA was obtained from S. mansoni– infected and noninfected snails. The results were visualized in silver stained polyacrylamide gels, revealing the presence of specific bands. The methodology has shown to be efficient, fast, and reproducible for Biomphalaria species identification and diagnosis of snails infected by S. mansoni during prepatent periods.
Marco A Mendez - One of the best experts on this subject based on the ideXlab platform.
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microgeographic differentiation among closely related species of Biomphalaria gastropoda planorbidae from the andean altiplano
Zoological Journal of the Linnean Society, 2013Co-Authors: Gonzalo A Collado, Marco A MendezAbstract:Direct development and water dependence entail limited vagility in freshwater fauna. In these organisms, the population structure is probably linked to restrictions imposed by the habitat. In this study we investigate the relative contribution of processes stimulating the divergence of populations of Biomphalaria costata (Biese, 1951) and Biomphalaria crequii (Courty, 1907), two freshwater snails occurring in two contiguous and fragmented closed basins from the Andean Altiplano using mitochondrial DNA (cytochrome c oxidase subunit I) sequences, shell morphometric and radular morphology. In order to clarify the species boundaries, a third allopatric species was included: Biomphalaria aymara Valdovinos & Stuardo, 1991. Molecular analyses recovered two distinct clades: one composed of B. aymara from the Isluga swamps and B. costata from Spring 1 in Salar de Carcote, the single spring occupied by this species, and another integrated by snails from 12 springs spread across the Salar de Carcote and the Salar de Ascotan assigned to B. crequii, originally described from the Salar de Ascotan. Unlike shell morphometrics, radular morphology was informative for distinguishing these species. The division of the lineages occurred in the Late Pleistocene. A subclade that includes snails from the southernmost springs in Salar de Ascotan suggests fragmentation of the distribution of B. crequii associated with landscape discontinuities. In addition to microvicariance signals, the private haplotypes scattered around both salt spans show that close-range dispersal is a common biogeographic process in this species. As evolutionary units, the single isolated and restricted population of B. costata has a high priority for conservation. © 2013 The Linnean Society of London
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phylogenetic relationships and taxonomy of altiplano populations of Biomphalaria gastropoda planorbidae inference from a multilocus approach
Zoological Journal of the Linnean Society, 2012Co-Authors: Gonzalo A Collado, Marco A MendezAbstract:Isolated closed basins provide a natural laboratory to study the differentiation among wild populations. Here we examined the phylogenetic relationships of the Southern Altiplano populations of Biomphalaria, a genus with medical importance, using nuclear (ITS1, ITS2) and mitochondrial (16S) ribosomal gene markers and a species of Helisoma as outgroup. Phylogenetic trees based on separate and combined analyses show that these populations form a particular lineage within Biomphalaria along with Biomphalaria peregrina (d'Orbigny, 1835) and Biomphalaria oligoza Paraense, 1974. The origin of this clade was estimated to have occurred in the middle Pleistocene. Molecular analyses showed that the nominal species Biomphalaria crequii (Courty, 1907) from the Salar de Ascotan and Biomphalaria costata (Biese, 1951) from the Salar de Carcote, previously synonymized with Biomphalaria andecola (d'Orbigny, 1835) and B. peregrina, respectively, are distinct taxa. Molecular data did not resolve the relationship of Biomphalaria aymara Valdovinos & Stuardo, 1991 from the Isluga swamps to other Biomphalaria species, but confirm that the populations from the Lauca and Huasco basins may represent a distinct undescribed species of Biomphalaria from the Southern Altiplano. Snails examined for trematodes were found to be positive in some Altiplano localities. © 2012 The Linnean Society of London, Zoological Journal of the Linnean Society, 2012, 165, 795–808.
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monophyly candidate species and vicariance in Biomphalaria snails mollusca planorbidae from the southern andean altiplano
Zoologica Scripta, 2011Co-Authors: Gonzalo A Collado, Irma Vila, Marco A MendezAbstract:Collado, G. A., Vila, I. & Mendez, M. A. (2011). Monophyly, candidate species and vicariance in Biomphalaria snails (Mollusca: Planorbidae) from the Southern Andean Altiplano. —Zoologica Scripta, 40, 613–622. The landscape of the Neotropical southern Andean Altiplano is characterized by a succession of closed basins originated from the Miocene to the Holocene. In this region, the number of species and phylogenetic relationships among freshwater snails of the genus Biomphalaria are uncertain. Here we obtained sequences of the mitochondrial gene cytochrome oxidase subunit I from 17 Altiplano populations, including topotypes of three nominal species, which were analyzed together with published sequences of the genus using different methods of phylogenetic reconstruction and a species of Helisoma as outgroup. The Altiplano populations conform a monophyletic group whose sister group is the Neotropical species Biomphalaria peregrina. Within this clade we recovered four main lineages well supported and congruent with geographical distributions. One clade includes topotypes restricted exclusively to the Ascotan basin, the type locality of the nominal species Biomphalaria crequii. A second clade includes sequences that correspond to topotypes restricted to the Isluga and Carcote basins, the type localities of the nominal species Biomphalaria aymara and Biomphalaria costata, respectively. Two monophyletic groups clustered snails restricted to several aquatic systems within the Caquena and Lauca basins, which may represent candidate species. The branching pattern of the sequences suggests that in the diversification of these snails, events of vicariance inferred in the Pleistocene have predominated over dispersal phenomena.
Roberta Lima Caldeira - One of the best experts on this subject based on the ideXlab platform.
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morphological and molecular characterization of invasive Biomphalaria straminea in southern china
Infectious Diseases of Poverty, 2018Co-Authors: Roberta Lima Caldeira, Mohamed R Habib, Yunhai Guo, Claire J Standley, Xiaonong ZhouAbstract:Schistosomiasis is a common parasitic disease designated as a neglected tropical disease by the World Health Organization. Schistosomiasis mansoni is a form of the disease that is caused by the digenean trematode Schistosoma mansoni, transmitted through Biomphalaria spp. as an intermediate host. Biomphalaria was introduced to Hong Kong, China in aquatic plants shipments coming from Brazil and the snail rapidly established its habitats in southern China. Earlier studies of Biomphalaria spp. introduced to southern China identified the snails as Biomphalaria straminea, one of the susceptible species implicated in S. mansoni transmission in South America. However, recent molecular investigations also indicated the presence of another South American species, B. kuhniana, which is refractory to infection. As such, it is important to identify accurately the species currently distributed in southern China, especially with emerging reports of active S. mansoni infections in Chinese workers returning from Africa. We combined morphological and molecular taxonomy tools to precisely identify Biomphalaria spp. distributed in Guangdong Province, southern China. In order to clearly understand the molecular profile of the species, we constructed a phylogeny using mtDNA data (COI and 16S rRNA sequences) from six populations of Biomphalaria spp. from Shenzhen City in Guangdong Province. In addition, we examined the external morphology of the shell and internal anatomy of the reproductive organs. Both morphological and molecular evidences indicated a close affinity between Biomphalaria spp. populations from Guangdong and B. straminea from Brazil. The shell morphology was roughly identical in all the populations collected with rounded whorls on one side and subangulated on the other, a smooth periphery, an egg-shaped aperture bowed to one side, and a deep umbilicus. The shape and number of prostate diverticula (ranged from 11.67 to 17.67) in Guangdong populations supports its close affinity to B. straminea rather than B. kuhniana. Molecular analysis did not conflict with morphological analysis. Little genetic differentiation was observed within Biomphalaria populations collected. Phylogenetic analysis of COI and 16S rRNA haplotypes from snails collected and B. straminea sequences from Brazil and China using Bayesian inference revealed that Guangdong populations were clustered in one clade with B. straminea from Hong Kong of China and B. straminea from Brazil indicating their close affinity to each other. Data obtained in the current study clearly show that the populations of Biomphalaria spp. investigated are B. straminea, and we assume that those snails were either introduced via passive dispersal from Hong Kong of China or as a result of multiple introduction routes from Brazil.
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Identification of Biomphalaria sp. and other freshwater snails in the large-scale water transposition project in the Northeast of Brazil
Universidade de São Paulo, 2018Co-Authors: Fernando Schemelzer De Moraes Bezerra, Roberta Lima Caldeira, Marta Cristhiany Cunha Pinheiro, José Damião Da Silva Filho, Issis Maria Nogueira De Castro, Mariana Silva Sousa, Albeniza Barbosa Cavalcante, Alberto Novaes Ramos JúniorAbstract:ABSTRACT The wide eco-bio-social intervention generated by the SaoFrancisco River Integration Project (PISF) may contribute to the dispersion or introduction of schistosomiasis intermediate hosts in areas without prior recording. The objective was to characterize the limnic malacofauna and its distribution along watersheds involved in the PISF. A cross-sectional study based on the collection of mollusks from 33 water bodies, from Aurora, Brejo Santo, Jaguaretama, Jaguaribara, Jati e Mauriti municipalities in the Ceara (CE) State was developed. The conchological characteristics were used to identify snails at the genus level. The snails of the genus Biomphalaria were analyzed for the presence of Schistosoma mansoni cercariae and the molecular identification (only mollusks from Brejo Santo-CE) for differentiation between species. The following species were found: Biomphalaria sp.; Drepanotrema sp.; Melanoides sp.; Physa sp.; and Pomacea sp. Pomacea sp. (75.8%) and Biomphalaria sp. (72.7%) were the most prevalent species. All municipalities showed Biomphalaria sp. Biomphalaria straminea (Porcos Stream) and Biomphalaria kuhniana was identified in the Boi 1 and Cipo reservoirs (Brejo Santo). The evaluated municipalities under the influence of the PISF present areas with potential for schistosomiasis transmission. It is necessary to intensify control actions and health surveillance in these areas
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use of molecular methods for the rapid mass detection of schistosoma mansoni platyhelminthes trematoda in Biomphalaria spp gastropoda planorbidae
Journal of Tropical Medicine, 2017Co-Authors: Roberta Lima Caldeira, Liana K Jannottipassos, Omar Dos Santos CarvalhoAbstract:The low stringency-polymerase chain reaction (LS-PCR) and loop-mediated isothermal amplification (LAMP) assays were used to detect the presence of S. mansoni DNA in (1) Brazilian intermediate hosts (Biomphalaria glabrata, B. straminea, and B. tenagophila) with patent S. mansoni infections, (2) B. glabrata snails with prepatent S. mansoni infections, (3) various mixtures of infected and noninfected snails; and (4) snails infected with other trematode species. The assays showed high sensitivity and specificity and could detect S. mansoni DNA when one positive snail was included in a pool of 1,000 negative specimens of Biomphalaria. These molecular approaches can provide a low-cost, effective, and rapid method for detecting the presence of S. mansoni in pooled samples of field-collected Biomphalaria. These assays should aid mapping of transmission sites in endemic areas, especially in low prevalence regions and improve schistosomiasis surveillance. It will be a useful tool to monitor low infection rates of snails in areas where control interventions are leading towards the elimination of schistosomiasis.
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Multiple alignment of the RIBOc domain of Drosha and Dicer proteins.
2017Co-Authors: Fábio Ribeiro Queiroz, Roberta Lima Caldeira, Paulo Marcos Zech Coelho, Luciana Maria Silva, Wander De Jesus Jeremias, Élio Hideo Babá, Matheus De Souza GomesAbstract:The highlighted residues, D (aspartic acid) and E (glutamic acid), which are highly conserved in the RIBOc domain of B. glabrata, and their orthologues are responsible for its catalytic activity. The sequences used for the Dicer family were as follows: BGLB002125-PA (Biomphalaria glabrata), XP_005106232.1 (Aplysia californica), NP_524453.1 (Drosophila melanogaster), NP_683750.2 (Mus musculus), XP_006618601.1 (Apis dorsata), XP_004523314.1 (Ceratitis capitata), XP_003745061.1 (Metaseiulus occidentalis), NP_498761.2 (Caenorhabditis elegans), NP_001154925.1 (Danio rerio), XP_868526.3 (Canis lupus familiaris), XP_008774532.1 (Rattus norvegicus), NP_976235.1 (Bos taurus), XP_005179924.1 (Musca domestica) and Smp_169750.2 (Schistosoma mansoni). The sequences used for the Drosha family were as follows: BGLB003167-PA (Biomphalaria glabrata), XP_008199088.1 (Tribolium castaneum), XP_006618766.1 (Apis dorsata), XP_006558454.1 (Apis mellifera), XP_003394274.1 (Bombus terrestris), NP_477436.1 (Drosophila melanogaster), XP_005248351.1 (Homo sapiens), XP_006520084.1 (Mus musculus), EKC20603.1 (Crassostrea gigas), NP_001122460.2 (Caenorhabditis elegans), NP_001103942.1 (Danio rerio), NP_001101125.2 (Rattus norvegicus), XP_854135.2 (Canis lupus familiaris), XP_591998.4 (Bos taurus), XP_005186977.1 (Musca domestica), NP_001006379.1 (Gallus gallus), Smp_142510.2 (Schistosoma mansoni) and Sjp_0048900.1 (Schistosoma japonicum).
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Multiple alignment of the PIWI domain of Ago-like and Piwi-like proteins.
2017Co-Authors: Fábio Ribeiro Queiroz, Roberta Lima Caldeira, Paulo Marcos Zech Coelho, Luciana Maria Silva, Wander De Jesus Jeremias, Élio Hideo Babá, Matheus De Souza GomesAbstract:The highlighted residues, D (aspartic acid) and H (Histidine), which are highly conserved in the PIWI domains of B. glabrata and their orthologues, are responsible for the catalytic activity. The sequences used for the Ago-like family were as follows: BGLB002396-PA (Biomphalaria glabrata), XP_005107587.1 (Aplysia californica), XP_009064000.1 (Lottia gigantea), EKC19600.1 (Crassostrea gigas), XP_003747659.1 (Metaseiulus occidentalis), XP_004529840.1 (Ceratitis capitata), NP_725341.1 (Drosophila melanogaster), XP_006622112.1 (Apis dorsata), XP_005175308.1 (Musca domestica), XP_003400152.1 (Bombus terrestris), NP_001257239.1 (Caenorhabditis elegans), Sjp_0044720.1 (Schistosoma japonicum), Smp_198380.1 (Schistosoma mansoni), NP_067608.1 (Rattus norvegicus), NP_001289151.1 (Danio rerio), XP_005629013.1 (Canis lupus familiaris), XP_004940123.1 (Gallus gallus), NP_991363.1 (Bos taurus), NP_036286.2 (Homo sapiens) and NP_700451.2 (Mus musculus). The sequences used for the Piwi-like family were as follows: BGLB010170-PA (Biomphalaria glabrata), XP_005096149.1 (Aplysia californica), XP_009064630.1 (Lottia gigantea), EKC35279.1 (Crassostrea gigas), XP_001641994.1 (Nematostella vectensis), NP_001274302.1 (Hydra vulgaris), NP_476875.1 (Drosophila melanogaster), XP_001652945.1 (Aedes aegypti), XP_003400353.1 (Bombus terrestris), XP_005183556.1 (Musca domestica), NP_004755.2 (Homo sapiens), NP_067286.1 (Mus musculus), NP_899181.1 (Danio rerio), XP_534638.2 (Canis lupus familiaris), XP_008764202.1 (Rattus norvegicus), NP_001092322.1 (Gallus gallus) and XP_618020.4 (Bos taurus).
Teofânia H D A Vidigal - One of the best experts on this subject based on the ideXlab platform.
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multiplex pcr for both identification of brazilian Biomphalaria species gastropoda planorbidae and diagnosis of infection by schistosoma mansoni trematoda schistosomatidae
Journal of Parasitology, 2006Co-Authors: Liana K Jannottipassos, Omar Dos Santos Carvalho, Kelly Grace Magalhaes, Teofânia H D A VidigalAbstract:A simple and single-step technique based on multiplex PCR (multiplex polymerase chain reaction) has been developed for simultaneous identification of Brazilian Biomphalaria species, the intermediate hosts of Schistosoma mansoni, and their diagnosis of infection by the trematode. We used species-specific primers directed both to the internal transcribed spacer 2 (ITS2) of ribosomal DNA from 3 of the S. mansoni host species and to the mitochondrial DNA (mtDNA) from the trematode. Those primers were used simultaneously in a single multiplex-PCR reaction, and template DNA was obtained from S. mansoni– infected and noninfected snails. The results were visualized in silver stained polyacrylamide gels, revealing the presence of specific bands. The methodology has shown to be efficient, fast, and reproducible for Biomphalaria species identification and diagnosis of snails infected by S. mansoni during prepatent periods.
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analysis of the first and second internal transcribed spacer sequences of the ribosomal dna in Biomphalaria tenagophila complex mollusca planorbidae
Memorias Do Instituto Oswaldo Cruz, 2004Co-Authors: Linus Spatz, Teofânia H D A Vidigal, Jessica C Kissinger, Edina R Pires, Rodrigo A F Redondo, Andrew J G Simpson, Omar Dos Santos CarvalhoAbstract:The first and second internal transcribed spacer regions (ITS1 and ITS2) of the ribosomal DNA of Biomphalaria tenagophila complex (B. tenagophila, B. occidentalis, and B. t. guaibensis) were sequenced and compared. The alignment lengths of these regions were about 655 bp and 481 bp, respectively. Phylogenetic relationships among the Biomphalaria species were inferred by Maximum Parsimony and Neighbor-joining methods. The phylogenetic trees produced, in most of the cases, were in accordance with morphological systematics and other molecular data previously obtained by polymerase chain reaction and restriction fragment length polymorphism analysis. The present results provide support for the proposal that B. tenagophila represents a complex comprising B. tenagophila, B. occidentalis and B. t. guaibensis.
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genetic variability and molecular identification of brazilian Biomphalaria species mollusca planorbidae
Parasitology, 2001Co-Authors: Omar Dos Santos Carvalho, Roberta Lima Caldeira, A J G Simpson, Teofânia H D A VidigalAbstract:Freshwater snails belonging to the genus Biomphalaria are intermediate hosts of the trematode Schistosoma mansoni in the Neotropical region and Africa. In Brazil, one subspecies and ten species of Biomphalaria have been identified: B. glabrata, B. tenagophila, B. straminea, B. occidentalis, B. peregrina, B. kuhniana, B. schrammi, B. amazonica, B. oligoza, B. intermedia and B.t. guaibensis. However, only the first three species are found naturally infected with S. mansoni. The classical identification of these planorbids is based on comparison of morphological characteristics of the shell and male and female reproductive organs, which is greatly complicated by the extensive intra-specific variation. Several molecular techniques have been used in studies on the identification, genetic structure as well as phylogenetic relationships between these groups of organisms. Using the randomly amplified polymorphic DNAs (RAPD) analysis we demonstrated that B. glabrata exhibits a remarkable degree of intra-specific polymorphism. Thus, the genetics of the snail host may be more important to the epidemiology of schistosomiasis than those of the parasite itself. Using the simple sequence repeat anchored polymerase chain reaction (SSR-PCR) in intra-populational and intra-specific studies we have demonstrated that snails belonging to the B. straminea complex (B. straminea, B. kuhniana and B. intermedia) clearly presented higher heterogeneity. Using the low stringency polymerase chain reaction (LS-PCR) technique we were able to separate B. glabrata from B. tenagophila and B. tenagophila from B. occidentalis. To separate all Brazilian Biomphalaria species we used the restriction fragment length polymorphism (PCR-RFLP) of the internal transcribed spacer region (ITS) of the DNA gene. The method also proved to be efficient for the specific identification of DNA extracted from snail eggs. Recently we have sequenced the ITS2 region for phylogenetic studies of all Biomphalaria snails from Brazil.
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phylogenetic relationships among brazilian Biomphalaria species mollusca planorbidae based upon analysis of ribosomal its2 sequences
Parasitology, 2000Co-Authors: Teofânia H D A Vidigal, Roberta Lima Caldeira, A J G Simpson, Jessica C Kissinger, Edina R Pires, E Monteiro, Omar Dos Santos CarvalhoAbstract:In spite of their abundance, widespread distribution and medical importance, the phylogenetic relationships among Biomphalaria snails have received relatively little attention. We have collected and studied 29 populations of snails obtained from different localities from Brazil. We have sequenced the ribosomal DNA second internal transcribed spacer (ITS2) from the following Biomphalaria species: B. glabrata, B. tenagophila tenagophila, B. occidentalis, B. straminea, B. peregrina, B. kuhniana, B. schrammi, B. amazonica, B. oligoza, B. intermedia and an outgroup species Helisoma duryi. The sequence from each species is unique. Three different methods of phylogenetic reconstruction were used (distance, maximum parsimony and maximum likelihood). The resulting phylogenetic trees obtained by these methods basically support current systematic relationships based on morphological characters alone. This study demonstrates that the ITS2 region contains markers useful for identification and determination of relationships among Biomphalaria species.
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molecular identification of similar species of the genus Biomphalaria mollusca planorbidae determined by a polymerase chain reaction restriction fragment length polymorphism
Memorias Do Instituto Oswaldo Cruz, 1998Co-Authors: Roberta Lima Caldeira, Teofânia H D A Vidigal, Andrew J G Simpson, Sonia Torquato Paulinelli, Omar Dos Santos CarvalhoAbstract:The freshwater snails Biomphalaria straminea, B. intermedia, B. kuhniana and B. peregrina, are morphologically similar; based on this similarity the first three species were therefore grouped in the complex B. straminea. The morphological identification of these species is based on characters such as vaginal wrinkling, relation between prepuce: penial sheath:deferens vas and number of muscle layers in the penis wall. In this study the polymerase chain reaction restriction fragment length polymorphism technique was used for molecular identification of these molluscs. This technique is based on the amplification of the internal transcribed spacer regions ITS1 e ITS2 of the ribosomal RNA gene and subsequent digestion of these fragments by restriction enzymes. Six enzymes were tested: Dde I, Mnl I, Hae III, Rsa I, Hpa II e Alu I. The restriction patterns obtained with DdeI presented the best profile for separation of the four species of Biomphalaria. The profiles obtained with all the enzymes were used to estimate the genetic distances among the species through analysis of common banding patterns.
Gonzalo A Collado - One of the best experts on this subject based on the ideXlab platform.
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microgeographic differentiation among closely related species of Biomphalaria gastropoda planorbidae from the andean altiplano
Zoological Journal of the Linnean Society, 2013Co-Authors: Gonzalo A Collado, Marco A MendezAbstract:Direct development and water dependence entail limited vagility in freshwater fauna. In these organisms, the population structure is probably linked to restrictions imposed by the habitat. In this study we investigate the relative contribution of processes stimulating the divergence of populations of Biomphalaria costata (Biese, 1951) and Biomphalaria crequii (Courty, 1907), two freshwater snails occurring in two contiguous and fragmented closed basins from the Andean Altiplano using mitochondrial DNA (cytochrome c oxidase subunit I) sequences, shell morphometric and radular morphology. In order to clarify the species boundaries, a third allopatric species was included: Biomphalaria aymara Valdovinos & Stuardo, 1991. Molecular analyses recovered two distinct clades: one composed of B. aymara from the Isluga swamps and B. costata from Spring 1 in Salar de Carcote, the single spring occupied by this species, and another integrated by snails from 12 springs spread across the Salar de Carcote and the Salar de Ascotan assigned to B. crequii, originally described from the Salar de Ascotan. Unlike shell morphometrics, radular morphology was informative for distinguishing these species. The division of the lineages occurred in the Late Pleistocene. A subclade that includes snails from the southernmost springs in Salar de Ascotan suggests fragmentation of the distribution of B. crequii associated with landscape discontinuities. In addition to microvicariance signals, the private haplotypes scattered around both salt spans show that close-range dispersal is a common biogeographic process in this species. As evolutionary units, the single isolated and restricted population of B. costata has a high priority for conservation. © 2013 The Linnean Society of London
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phylogenetic relationships and taxonomy of altiplano populations of Biomphalaria gastropoda planorbidae inference from a multilocus approach
Zoological Journal of the Linnean Society, 2012Co-Authors: Gonzalo A Collado, Marco A MendezAbstract:Isolated closed basins provide a natural laboratory to study the differentiation among wild populations. Here we examined the phylogenetic relationships of the Southern Altiplano populations of Biomphalaria, a genus with medical importance, using nuclear (ITS1, ITS2) and mitochondrial (16S) ribosomal gene markers and a species of Helisoma as outgroup. Phylogenetic trees based on separate and combined analyses show that these populations form a particular lineage within Biomphalaria along with Biomphalaria peregrina (d'Orbigny, 1835) and Biomphalaria oligoza Paraense, 1974. The origin of this clade was estimated to have occurred in the middle Pleistocene. Molecular analyses showed that the nominal species Biomphalaria crequii (Courty, 1907) from the Salar de Ascotan and Biomphalaria costata (Biese, 1951) from the Salar de Carcote, previously synonymized with Biomphalaria andecola (d'Orbigny, 1835) and B. peregrina, respectively, are distinct taxa. Molecular data did not resolve the relationship of Biomphalaria aymara Valdovinos & Stuardo, 1991 from the Isluga swamps to other Biomphalaria species, but confirm that the populations from the Lauca and Huasco basins may represent a distinct undescribed species of Biomphalaria from the Southern Altiplano. Snails examined for trematodes were found to be positive in some Altiplano localities. © 2012 The Linnean Society of London, Zoological Journal of the Linnean Society, 2012, 165, 795–808.
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monophyly candidate species and vicariance in Biomphalaria snails mollusca planorbidae from the southern andean altiplano
Zoologica Scripta, 2011Co-Authors: Gonzalo A Collado, Irma Vila, Marco A MendezAbstract:Collado, G. A., Vila, I. & Mendez, M. A. (2011). Monophyly, candidate species and vicariance in Biomphalaria snails (Mollusca: Planorbidae) from the Southern Andean Altiplano. —Zoologica Scripta, 40, 613–622. The landscape of the Neotropical southern Andean Altiplano is characterized by a succession of closed basins originated from the Miocene to the Holocene. In this region, the number of species and phylogenetic relationships among freshwater snails of the genus Biomphalaria are uncertain. Here we obtained sequences of the mitochondrial gene cytochrome oxidase subunit I from 17 Altiplano populations, including topotypes of three nominal species, which were analyzed together with published sequences of the genus using different methods of phylogenetic reconstruction and a species of Helisoma as outgroup. The Altiplano populations conform a monophyletic group whose sister group is the Neotropical species Biomphalaria peregrina. Within this clade we recovered four main lineages well supported and congruent with geographical distributions. One clade includes topotypes restricted exclusively to the Ascotan basin, the type locality of the nominal species Biomphalaria crequii. A second clade includes sequences that correspond to topotypes restricted to the Isluga and Carcote basins, the type localities of the nominal species Biomphalaria aymara and Biomphalaria costata, respectively. Two monophyletic groups clustered snails restricted to several aquatic systems within the Caquena and Lauca basins, which may represent candidate species. The branching pattern of the sequences suggests that in the diversification of these snails, events of vicariance inferred in the Pleistocene have predominated over dispersal phenomena.