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Jong Pil Park - One of the best experts on this subject based on the ideXlab platform.
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Mycelial growth and exo‐Biopolymer Production by submerged culture of various edible mushrooms under different media
Letters in Applied Microbiology, 2020Co-Authors: H J Hwang, Jong Pil Park, C H SongAbstract:Aims: The effect of synthetic media on the submerged mycelial growth and exo-Biopolymer Production in various edible mushrooms was investigated in shake flask culture. Methods and Results: Among 19 mushrooms examined, the relatively high yield in mycelial biomass and exo-Biopolymer Production was achieved in potato malt peptone (PMP) medium. In particular, Ganoderma lucidum NO. 1 and Phellinus linteus KCTC 6190 showed favourable growth in PMP medium with exo-Biopolymer concentration of 1170 and 1520 mg l−1, respectively. Conclusions: Enhanced exo-Biopolymer Production was achieved from Ganoderma lucidum NO. 1 and Phellinus linteus KCTC 6190 in a 5L batch fermentor, indicating approximately 5000 and 2410 mg l−1, respectively. Significance and Impact of the Study: The exo-Biopolymer Production and mycelial growth from various mushrooms were found to be strongly controlled by different complex media.
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stimulatory effect of plant oils and fatty acids on the exo Biopolymer Production in cordyceps militaris
Enzyme and Microbial Technology, 2002Co-Authors: Jong Pil Park, Hyejin HwangAbstract:Abstract The exo-Biopolymer Production and mycelial growth were substantially increased (almost tripled) by supplementation of certain vegetable oils into the medium. In particular, sunflower oil at the level of 2% led to a significant increase in exo-Biopolymer concentration from 2.3 to 7.5 g/l, while the addition of 4% olive oil dramatically increased mycelial biomass from 5.8 to 19.0 g/l. To elucidate which components in those oils increased Biopolymer Production, effects of major fatty acids that constitute plant oils were further studied. Among fatty acids tested, addition of 2% oleic acid and palmitic acid markedly stimulated the Biopolymer Production (enhancement factors were 6 and 5.5, respectively), whereas linoleic acid drastically suppressed both mycelial growth and exo-Biopolymer Production.
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effect of agitation intensity on the exo Biopolymer Production and mycelial morphology in cordyceps militaris
Letters in Applied Microbiology, 2002Co-Authors: Jong Pil Park, H J Hwang, C H SongAbstract:Aims: The influence of agitation intensity on Cordyceps militaris morphology and exo-Biopolymer Production was investigated in a 5 litre stirred vessel using a six-blade Rushton turbine impeller. Methods and Results: The mycelial morphology of C. militaris was characterized by means of image analysis, which included mean diameter, circularity, roughness and compactness of the pellets. The morphological parameters of the pellets grown under different stirring conditions were significantly different, which correspondingly altered exo-Biopolymer Production yields. Conclusions: The compactness of the pellets was found to be the most critical parameter affecting exo-Biopolymer biosynthesis; more compact pellets were formed at 150 rev min–1 with maximum exo-Biopolymer Production (15 g l–1). Significance and Impact of the Study: The results of this study suggest that morphological change of pellets is a good indicator for identifying the cell activity for exo-Biopolymer Production.
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effect of aeration rate on the mycelial morphology and exo Biopolymer Production in cordyceps militaris
Process Biochemistry, 2002Co-Authors: Jong Pil Park, Hyejin Hwang, C H SongAbstract:The influence of aeration rate on Cordyceps militaris morphology and exo-Biopolymer Production was investigated in a 5-l jar fermentor. The mycelial morphology of C. militaris was characterized by image analysis, which included mean diameter, circularity, roughness, and compactness of the pellets. Cells were observed to form mainly pellets during the entire culture period irrespective of aeration conditions. There existed a notable variation in morphological parameters between the pellets grown on different aeration conditions, by which exo-Biopolymer Production yields were correspondingly altered. The mean diameter and compactness of the pellets indicated higher values at 2 vvm (volume of air per volume of culture per minute), which was closely related to exo-Biopolymer biosynthesis. The more compact pelleted form was favourable for exo-Biopolymer Production. Under extremely low and high aeration conditions (e.g. 0.5 and 4 vvm), severe deformations of pellets (autolysis of core and shaving off the outer hairy region) were observed at the later stages of fermentation associated with a decrease in morphological parameters. © 2002 Elsevier Science Ltd. All rights reserved.
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economic aspects of Biopolymer Production
Biopolymers Online, 2002Co-Authors: Si Jae Park, Jong Pil ParkAbstract:Introduction Historical Outline Microbial Polysaccharides Bacterial Cellulose (β-d-Glucan) Pullulan (α-d-Glucan) Xanthan Poly-γ-glutamic acid (γ-PGA) Production of γ-PGA Recovery of γ-PGA Polyhydroxyalkanoates (PHAs) Poly(3-hydroxybutyrate) Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) Medium-chain-length Poly(3-hydroxyalkanoates) Outlook and Perspectives Patents Keywords: Biopolymers; polysaccharides; pullulan; poly-γ-glutamic acid; polyhydroxyalkanoates (PHAs); economic aspects
C H Song - One of the best experts on this subject based on the ideXlab platform.
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Mycelial growth and exo‐Biopolymer Production by submerged culture of various edible mushrooms under different media
Letters in Applied Microbiology, 2020Co-Authors: H J Hwang, Jong Pil Park, C H SongAbstract:Aims: The effect of synthetic media on the submerged mycelial growth and exo-Biopolymer Production in various edible mushrooms was investigated in shake flask culture. Methods and Results: Among 19 mushrooms examined, the relatively high yield in mycelial biomass and exo-Biopolymer Production was achieved in potato malt peptone (PMP) medium. In particular, Ganoderma lucidum NO. 1 and Phellinus linteus KCTC 6190 showed favourable growth in PMP medium with exo-Biopolymer concentration of 1170 and 1520 mg l−1, respectively. Conclusions: Enhanced exo-Biopolymer Production was achieved from Ganoderma lucidum NO. 1 and Phellinus linteus KCTC 6190 in a 5L batch fermentor, indicating approximately 5000 and 2410 mg l−1, respectively. Significance and Impact of the Study: The exo-Biopolymer Production and mycelial growth from various mushrooms were found to be strongly controlled by different complex media.
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effect of agitation intensity on the exo Biopolymer Production and mycelial morphology in cordyceps militaris
Letters in Applied Microbiology, 2002Co-Authors: Jong Pil Park, H J Hwang, C H SongAbstract:Aims: The influence of agitation intensity on Cordyceps militaris morphology and exo-Biopolymer Production was investigated in a 5 litre stirred vessel using a six-blade Rushton turbine impeller. Methods and Results: The mycelial morphology of C. militaris was characterized by means of image analysis, which included mean diameter, circularity, roughness and compactness of the pellets. The morphological parameters of the pellets grown under different stirring conditions were significantly different, which correspondingly altered exo-Biopolymer Production yields. Conclusions: The compactness of the pellets was found to be the most critical parameter affecting exo-Biopolymer biosynthesis; more compact pellets were formed at 150 rev min–1 with maximum exo-Biopolymer Production (15 g l–1). Significance and Impact of the Study: The results of this study suggest that morphological change of pellets is a good indicator for identifying the cell activity for exo-Biopolymer Production.
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effect of aeration rate on the mycelial morphology and exo Biopolymer Production in cordyceps militaris
Process Biochemistry, 2002Co-Authors: Jong Pil Park, Hyejin Hwang, C H SongAbstract:The influence of aeration rate on Cordyceps militaris morphology and exo-Biopolymer Production was investigated in a 5-l jar fermentor. The mycelial morphology of C. militaris was characterized by image analysis, which included mean diameter, circularity, roughness, and compactness of the pellets. Cells were observed to form mainly pellets during the entire culture period irrespective of aeration conditions. There existed a notable variation in morphological parameters between the pellets grown on different aeration conditions, by which exo-Biopolymer Production yields were correspondingly altered. The mean diameter and compactness of the pellets indicated higher values at 2 vvm (volume of air per volume of culture per minute), which was closely related to exo-Biopolymer biosynthesis. The more compact pelleted form was favourable for exo-Biopolymer Production. Under extremely low and high aeration conditions (e.g. 0.5 and 4 vvm), severe deformations of pellets (autolysis of core and shaving off the outer hairy region) were observed at the later stages of fermentation associated with a decrease in morphological parameters. © 2002 Elsevier Science Ltd. All rights reserved.
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mycelial growth and exo Biopolymer Production by submerged culture of various edible mushrooms under different media
Letters in Applied Microbiology, 2002Co-Authors: H J Hwang, Jong Pil Park, C H SongAbstract:Aims: The effect of synthetic media on the submerged mycelial growth and exo-Biopolymer Production in various edible mushrooms was investigated in shake flask culture. Methods and Results: Among 19 mushrooms examined, the relatively high yield in mycelial biomass and exo-Biopolymer Production was achieved in potato malt peptone (PMP) medium. In particular, Ganoderma lucidum NO. 1 and Phellinus linteus KCTC 6190 showed favourable growth in PMP medium with exo-Biopolymer concentration of 1170 and 1520 mg l−1, respectively. Conclusions: Enhanced exo-Biopolymer Production was achieved from Ganoderma lucidum NO. 1 and Phellinus linteus KCTC 6190 in a 5L batch fermentor, indicating approximately 5000 and 2410 mg l−1, respectively. Significance and Impact of the Study: The exo-Biopolymer Production and mycelial growth from various mushrooms were found to be strongly controlled by different complex media.
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effect of substrate concentration on broth rheology and fungal morphology during exo Biopolymer Production by paecilomyces japonica in a batch bioreactor
Enzyme and Microbial Technology, 2001Co-Authors: Jayanta Sinha, Jong Pil Park, C H SongAbstract:The influence of pellet morphology of Paecilomyces japonica on fermentation broth rheology and exo– Biopolymer Production was investigated. The substrate concentration was varied between 20 and 80 g/liter while running the fermentation at a high aeration of 3 vvm. The specific growth rate of Paecilomyces japonicaand exo– Biopolymer Production was found to be optimum with a substrate concentration of 40 g/liter of sucrose. Rapid formation of pellets was observed after the first day of fermentation, which slowly increased in size, hairiness and roughness. This, together with the increase in biomass concentration, altered the transport characteristics and the broth rheology to pseudoplastic nature, in turn, influenced exo– Biopolymer Production. At mild agitation, high aeration and optimum substrate concentration, pellets were the most predominant morphological form compared to free mycelia. Compact pellets with least hairiness and roughness, which improved the flow characteristics of the fermentation broth were observed at 40 g/liter of sucrose and hence regarded as the most productive morphological form. © 2001 Elsevier Science Inc. All rights reserved.
Chunping Xu - One of the best experts on this subject based on the ideXlab platform.
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Optimization of submerged-culture conditions for mycelial growth and exo-Biopolymer Production by Auricularia polytricha (wood ears fungus) using the methods of uniform design and regression analysis.
Biotechnology and applied biochemistry, 2020Co-Authors: Chunping XuAbstract:This paper is concerned with the optimization of submerged culture conditions for mycelial growth and exo-Biopolymer Production by Auricularia polytricha by one-factor-at-a-time and uniform design (UD) methods. First, the one-factor-at-a-time method was adopted to investigate the effects of environmental factors (i.e., initial pH and temperature) and variables of medium components (i.e., carbon, nitrogen and mineral sources) on mycelial growth and exo-Biopolymer Production. Sucrose, yeast extract, and K2HPO4 were identified to be the most suitable carbon, nitrogen, and mineral sources, respectively. The optimal temperature and initial pH for mycelial growth and exo-Biopolymer Production were found to be 25 degrees C and 5.0, respectively. Subsequently, the concentrations of sucrose, yeast extract, and K2HPO4 were optimized using the UD method. The optimal concentrations for the enhanced Production were determined as 6% (w/v) sucrose, 2.5% (w/v) yeast extract, and 0.3% (w/v) K2HPO4 for mycelial yield, and 6% (w/v) sucrose, 1.28% (w/v) yeast extract, and 0.3% (w/v) K2HPO4 for exo-Biopolymer Production, respectively. Subsequent experiments confirmed the validity of the models. This optimization strategy in shake-flask culture led to a mycelial yield of 6.14 g/l, and exo-Biopolymer Production of 2.12 g/l, respectively, which were considerably higher than those obtained in the preliminary studies. By using the optimized medium, the maximum concentrations of mycelial biomass and exo-Biopolymer in a 5 litre stirred-tank bioreactor indicated 35.3 g/l and 3.1 g/l, respectively.
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optimization of physical parameters for exo Biopolymer Production in submerged mycelial cultures of two entomopathogenic fungi paecilomyces japonica and paecilomyces tenuipes
Letters in Applied Microbiology, 2006Co-Authors: Chunping Xu, Jayanta SinhaAbstract:Aims: In the present study, two different optimization techniques were used to determine the suitable operating parameters for exo–Biopolymer Production in submerged mycelial cultures of two entomopathogenic fungi Paecilomyces japonica and Paecilomyces tenuipes. Methods and Results: First, the rotating simplex method, a nonstatistical optimization technique, was employed to obtain the best combination of physical parameters (viz. pH, agitation intensity, aeration rate) for maximum exo–Biopolymer Production by P. japonica in a batch bioreactor. The optimal combination was determined to be a pH of 8AE06, an aeration of 3 vvm, without any impeller agitation, producing a 17-time increase in exopolymer Production (34AE 5gl )1 ) when compared with that achieved in unoptimized flask cultures. Second, the uniform design method, a statistical optimization technique, was employed to determine the best operating parameters for submerged culture of P. tenuipes. The optimal combination for mycelial growth was determined to be a pH of 4AE88, an aeration of 2 vvm and an agitation of 350 rpm, while a pH of 4, an aeration of 2 vvm and an agitation of 150 rpm was best for exo–Biopolymer Production. Conclusions: The exo–Biopolymer Production in P. japonica optimized by the rotating simplex method was strikingly improved (max. 34AE 5gl )1 ), and the exo–Biopolymer Production in P. tenuipes optimized by the uniform design method was also significantly increased (max. 3AE 4gl )1 ). Significance and Impact of the Study: The successful application of these two different optimization techniques in this study implies that these methods are worthy of applying to other fermentation systems for the Production of bioactive mycelial biomass and exo–Biopolymers in liquid culture of higher fungi.
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optimization of submerged culture conditions for mycelial growth and exo Biopolymer Production by auricularia polytricha wood ears fungus using the methods of uniform design and regression analysis
Biotechnology and Applied Biochemistry, 2003Co-Authors: Chunping XuAbstract:This paper is concerned with the optimization of submerged culture conditions for mycelial growth and exo-Biopolymer Production by Auricularia polytricha by one-factor-at-a-time and uniform design (UD) methods. First, the one-factor-at-a-time method was adopted to investigate the effects of environmental factors (i.e., initial pH and temperature) and variables of medium components (i.e., carbon, nitrogen and mineral sources) on mycelial growth and exo-Biopolymer Production. Sucrose, yeast extract, and K2HPO4 were identified to be the most suitable carbon, nitrogen, and mineral sources, respectively. The optimal temperature and initial pH for mycelial growth and exoBiopolymer Production were found to be 25 ◦ Ca nd 5.0, respectively. Subsequently, the concentrations of sucrose, yeast extract, and K2HPO4 were optimized using the UD method. The optimal concentrations for the enhanced Production were determined as 6% (w/v) sucrose, 2.5% (w/v) yeast extract, and 0.3% (w/v) K2HPO4 fo rm ycelial yield, and 6% (w/v) sucrose, 1.28% (w/v) yeast extract, and 0.3% (w/v) K2HPO4 fo re xo-Biopolymer Production, respectively. Subsequent experiments confirmed the validity of the models. This optimization strategy in shake-flask culture led to a mycelial yield of 6.14 g/l, and exoBiopolymer Production of 2.12 g/l, respectively, which were considerably higher than those obtained in the preliminary studies. By using the optimized medium, the maximum concentrations of mycelial biomass and exo-Biopolymer in a 5 litre stirred-tank bioreactor indicated 35.3 g/l and 3.1 g/l, respectively.
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Optimization of submerged‐culture conditions for mycelial growth and exo‐Biopolymer Production by Auricularia polytricha (wood ears fungus) using the methods of uniform design and regression analysis
Biotechnology and Applied Biochemistry, 2003Co-Authors: Chunping XuAbstract:This paper is concerned with the optimization of submerged culture conditions for mycelial growth and exo-Biopolymer Production by Auricularia polytricha by one-factor-at-a-time and uniform design (UD) methods. First, the one-factor-at-a-time method was adopted to investigate the effects of environmental factors (i.e., initial pH and temperature) and variables of medium components (i.e., carbon, nitrogen and mineral sources) on mycelial growth and exo-Biopolymer Production. Sucrose, yeast extract, and K2HPO4 were identified to be the most suitable carbon, nitrogen, and mineral sources, respectively. The optimal temperature and initial pH for mycelial growth and exoBiopolymer Production were found to be 25 ◦ Ca nd 5.0, respectively. Subsequently, the concentrations of sucrose, yeast extract, and K2HPO4 were optimized using the UD method. The optimal concentrations for the enhanced Production were determined as 6% (w/v) sucrose, 2.5% (w/v) yeast extract, and 0.3% (w/v) K2HPO4 fo rm ycelial yield, and 6% (w/v) sucrose, 1.28% (w/v) yeast extract, and 0.3% (w/v) K2HPO4 fo re xo-Biopolymer Production, respectively. Subsequent experiments confirmed the validity of the models. This optimization strategy in shake-flask culture led to a mycelial yield of 6.14 g/l, and exoBiopolymer Production of 2.12 g/l, respectively, which were considerably higher than those obtained in the preliminary studies. By using the optimized medium, the maximum concentrations of mycelial biomass and exo-Biopolymer in a 5 litre stirred-tank bioreactor indicated 35.3 g/l and 3.1 g/l, respectively.
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optimization of submerged culture conditions for mycelial growth and exo Biopolymer Production by paecilomyces tenuipes c240
Process Biochemistry, 2003Co-Authors: Chunping Xu, Hyejin Hwang, Jangwon ChoiAbstract:This paper is concerned with optimization of submerged culture conditions for mycelial growth and exo-Biopolymer Production by Paecilomyces tenuipes C240 by one-factor-at-a-time and orthogonal matrix methods. The one-factor-at-a-time method was adopted to investigate the effects of medium components (i.e. carbon, nitrogen, and mineral sources) and environmental factors (i.e. initial pH and temperature) on mycelial growth and exo-Biopolymer Production. Among these variables, glucose, KNO3 ,K 2HPO4, and MgSO4 were identified to be the most suitable carbon, nitrogen, and mineral sources, respectively. The optimal temperature and initial pH for mycelial growth and exo-Biopolymer Production were 28 8C and 6.0, respectively. Subsequently, the concentration of glucose, KNO3 ,K 2HPO4, and MgSO4 were optimized using the orthogonal matrix method. The effects of media composition on the mycelial growth of P. tenuipes C240 were in the order of glucose � /K2HPO4� /KNO3� /MgSO4, and those on exo-Biopolymer Production were in the order of glucose � /K2HPO4 � /MgSO4� /KNO3. The optimal concentration for enhanced Production were determined as 4 g/l glucose, 0.6 g/l KNO3, 0.1 g/l K2HPO4, and 0.1 g/l MgSO4 / 5H2O for mycelial yield, and 3 g/l glucose, 0.4 g/l KNO3, 0.1 g/l K2HPO4, and 0.1 g/l MgSO4 / 5H2O for exo-Biopolymer Production, respectively. The subsequent verification experiments confirmed the validity of the models. This optimization strategy in shake flask culture lead to a mycelial yield of 10.18 g/ l, and exo-Biopolymer Production of 1.89 g/l, respectively, which were considerably higher than those obtained in preliminary studies. Under optimal culture conditions, the maximum exo-Biopolymer concentration in a 5 l stirred-tank bioreactor was 2.36 g/l. # 2002 Elsevier Science Ltd. All rights reserved.
Mattias Hjort - One of the best experts on this subject based on the ideXlab platform.
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integration of Biopolymer Production with process water treatment at a sugar factory
New Biotechnology, 2014Co-Authors: Simon Anterrieu, T. Alexandersson, B. Geurkink, Luca Quadri, Inez Dinkla, Simon Bengtsson, Anton Karlsson, Monica V Arcoshernandez, Fernando Morgansagastume, Mattias HjortAbstract:The present investigation has focused on generating a surplus denitrifying biomass with high polyhydroxyalkanoate (PHA) producing potential while maintaining water treatment performance in biological nitrogen removal. The motivation for the study was to examine integration of PHA Production into the water treatment and residuals management needs at the Suiker Unie sugar beet factory in Groningen, the Netherlands. At the factory, process waters are treated in nitrifying–denitrifying sequencing batch reactors (SBRs) to remove nitrogen found in condensate. Organic slippage (COD) in waters coming from beet washing is the substrate used for denitrification. The full-scale SBR was mimicked at laboratory scale. In two parallel laboratory scale SBRs, a mixed-culture biomass selection strategy of anoxic-feast and aerobic-famine was investigated using the condensate and wash water from Suiker Unie. One laboratory SBR was operated as conventional activated sludge with long solids retention time similar to the full-scale (SRT >16 days) while the other SBR was a hybrid biofilm-activated sludge (IFAS) process with short SRT (4–6 days) for the suspended solids. Both SBRs were found to produce biomass with augmented PHA Production potential while sustaining process water treatment for carbon, nitrogen and phosphorus for the factory process waters. PHA producing potential in excess of 60 percent g-PHA/g-VSS was achieved with the lab scale surplus biomass. Surplus biomass of low (4–6 days) and high (>16 days) solids retention time yielded similar results in PHA accumulation potential. However, nitrification performance was found to be more robust for the IFAS SBR. Assessment of the SBR microbial ecology based on 16sDNA and selected PHA synthase genes at full-scale in comparison to biomass from the laboratory scale SBRs suggested that the full-scale process was enriched with a PHA storing microbial community. However, structure–function relationships based on RNA levels for the selected PHA synthases could not be established and, towards this ambition, it is speculated that a wider representation of PHA synthesases would need to be monitored. Additionally at the factory, beet tail press waters coming from the factory beet residuals management activities are available as a carbon source for PHA accumulation. At pilot scale, beet tail press waters were shown to provide a suitable carbon source for mixed culture PHA Production in spite of otherwise being of relatively low organic strength (≤10 g-COD/L). A copolymer of 3-hydroxybutyrate with 3-hydroxyvalerate (PHBV with 15% HV on a molar basis) of high thermal stability and high weight average molecular mass (980 kDa) was produced from the beet tail press water. The mixed culture accumulation process sustained PHA storage with parallel biomass growth of PHA storing bacteria suggesting a strategy to further leverage the utilization of surplus functional biomass from biological treatment systems. Integration of PHA Production into the existing factory water management by using surplus biomass from condensate water treatment and press waters from beet residuals processing was found to be a feasible strategy for Biopolymer Production.
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Integration of Biopolymer Production with process water treatment at a sugar factory
New Biotechnology, 2014Co-Authors: Simon Anterrieu, T. Alexandersson, B. Geurkink, Luca Quadri, Inez Dinkla, Simon Bengtsson, Monica Arcos-hernandez, Anton Karlsson, Fernando Morgan-sagastume, Mattias HjortAbstract:The present investigation has focused on generating a surplus denitrifying biomass with high polyhydroxyalkanoate (PHA) producing potential while maintaining water treatment performance in biological nitrogen removal. The motivation for the study was to examine integration of PHA Production into the water treatment and residuals management needs at the Suiker Unie sugar beet factory in Groningen, the Netherlands. At the factory, process waters are treated in nitrifying-denitrifying sequencing batch reactors (SBRs) to remove nitrogen found in condensate. Organic slippage (COD) in waters coming from beet washing is the substrate used for denitrification. The full-scale SBR was mimicked at laboratory scale. In two parallel laboratory scale SBRs, a mixed-culture biomass selection strategy of anoxic-feast and aerobic-famine was investigated using the condensate and wash water from Suiker Unie. One laboratory SBR was operated as conventional activated sludge with long solids retention time similar to the full-scale (SRT >16 days) while the other SBR was a hybrid biofilm-activated sludge (IFAS) process with short SRT (4-6 days) for the suspended solids. Both SBRs were found to produce biomass with augmented PHA Production potential while sustaining process water treatment for carbon, nitrogen and phosphorus for the factory process waters. PHA producing potential in excess of 60 percent g-PHA/g-VSS was achieved with the lab scale surplus biomass. Surplus biomass of low (4-6 days) and high (>16 days) solids retention time yielded similar results in PHA accumulation potential. However, nitrification performance was found to be more robust for the IFAS SBR. Assessment of the SBR microbial ecology based on 16sDNA and selected PHA synthase genes at full-scale in comparison to biomass from the laboratory scale SBRs suggested that the full-scale process was enriched with a PHA storing microbial community. However, structure-function relationships based on RNA levels for the selected PHA synthases could not be established and, towards this ambition, it is speculated that a wider representation of PHA synthesases would need to be monitored. Additionally at the factory, beet tail press waters coming from the factory beet residuals management activities are available as a carbon source for PHA accumulation. At pilot scale, beet tail press waters were shown to provide a suitable carbon source for mixed culture PHA Production in spite of otherwise being of relatively low organic strength (≤10. g-COD/L). A copolymer of 3-hydroxybutyrate with 3-hydroxyvalerate (PHBV with 15% HV on a molar basis) of high thermal stability and high weight average molecular mass (980. kDa) was produced from the beet tail press water. The mixed culture accumulation process sustained PHA storage with parallel biomass growth of PHA storing bacteria suggesting a strategy to further leverage the utilization of surplus functional biomass from biological treatment systems. Integration of PHA Production into the existing factory water management by using surplus biomass from condensate water treatment and press waters from beet residuals processing was found to be a feasible strategy for Biopolymer Production. © 2013 Elsevier B.V.
Hyejin Hwang - One of the best experts on this subject based on the ideXlab platform.
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optimization of submerged culture conditions for mycelial growth and exo Biopolymer Production by paecilomyces tenuipes c240
Process Biochemistry, 2003Co-Authors: Chunping Xu, Hyejin Hwang, Jangwon ChoiAbstract:This paper is concerned with optimization of submerged culture conditions for mycelial growth and exo-Biopolymer Production by Paecilomyces tenuipes C240 by one-factor-at-a-time and orthogonal matrix methods. The one-factor-at-a-time method was adopted to investigate the effects of medium components (i.e. carbon, nitrogen, and mineral sources) and environmental factors (i.e. initial pH and temperature) on mycelial growth and exo-Biopolymer Production. Among these variables, glucose, KNO3 ,K 2HPO4, and MgSO4 were identified to be the most suitable carbon, nitrogen, and mineral sources, respectively. The optimal temperature and initial pH for mycelial growth and exo-Biopolymer Production were 28 8C and 6.0, respectively. Subsequently, the concentration of glucose, KNO3 ,K 2HPO4, and MgSO4 were optimized using the orthogonal matrix method. The effects of media composition on the mycelial growth of P. tenuipes C240 were in the order of glucose � /K2HPO4� /KNO3� /MgSO4, and those on exo-Biopolymer Production were in the order of glucose � /K2HPO4 � /MgSO4� /KNO3. The optimal concentration for enhanced Production were determined as 4 g/l glucose, 0.6 g/l KNO3, 0.1 g/l K2HPO4, and 0.1 g/l MgSO4 / 5H2O for mycelial yield, and 3 g/l glucose, 0.4 g/l KNO3, 0.1 g/l K2HPO4, and 0.1 g/l MgSO4 / 5H2O for exo-Biopolymer Production, respectively. The subsequent verification experiments confirmed the validity of the models. This optimization strategy in shake flask culture lead to a mycelial yield of 10.18 g/ l, and exo-Biopolymer Production of 1.89 g/l, respectively, which were considerably higher than those obtained in preliminary studies. Under optimal culture conditions, the maximum exo-Biopolymer concentration in a 5 l stirred-tank bioreactor was 2.36 g/l. # 2002 Elsevier Science Ltd. All rights reserved.
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stimulatory effect of plant oils and fatty acids on the exo Biopolymer Production in cordyceps militaris
Enzyme and Microbial Technology, 2002Co-Authors: Jong Pil Park, Hyejin HwangAbstract:Abstract The exo-Biopolymer Production and mycelial growth were substantially increased (almost tripled) by supplementation of certain vegetable oils into the medium. In particular, sunflower oil at the level of 2% led to a significant increase in exo-Biopolymer concentration from 2.3 to 7.5 g/l, while the addition of 4% olive oil dramatically increased mycelial biomass from 5.8 to 19.0 g/l. To elucidate which components in those oils increased Biopolymer Production, effects of major fatty acids that constitute plant oils were further studied. Among fatty acids tested, addition of 2% oleic acid and palmitic acid markedly stimulated the Biopolymer Production (enhancement factors were 6 and 5.5, respectively), whereas linoleic acid drastically suppressed both mycelial growth and exo-Biopolymer Production.
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effect of aeration rate on the mycelial morphology and exo Biopolymer Production in cordyceps militaris
Process Biochemistry, 2002Co-Authors: Jong Pil Park, Hyejin Hwang, C H SongAbstract:The influence of aeration rate on Cordyceps militaris morphology and exo-Biopolymer Production was investigated in a 5-l jar fermentor. The mycelial morphology of C. militaris was characterized by image analysis, which included mean diameter, circularity, roughness, and compactness of the pellets. Cells were observed to form mainly pellets during the entire culture period irrespective of aeration conditions. There existed a notable variation in morphological parameters between the pellets grown on different aeration conditions, by which exo-Biopolymer Production yields were correspondingly altered. The mean diameter and compactness of the pellets indicated higher values at 2 vvm (volume of air per volume of culture per minute), which was closely related to exo-Biopolymer biosynthesis. The more compact pelleted form was favourable for exo-Biopolymer Production. Under extremely low and high aeration conditions (e.g. 0.5 and 4 vvm), severe deformations of pellets (autolysis of core and shaving off the outer hairy region) were observed at the later stages of fermentation associated with a decrease in morphological parameters. © 2002 Elsevier Science Ltd. All rights reserved.